Mobile device applied to nucleic acid extraction system and control method thereof

Abstract

A mobile device applied to a nucleic acid extraction system comprises: a workbench; the moving assembly is arranged on the workbench; the control element is arranged on the moving assembly and is electrically connected with the moving assembly; the first motor is arranged on the moving assembly and is electrically connected with the control element; the first rotating rod is connected with the first motor; the second motor is arranged on the moving assembly and is electrically connected with the control element; the second rotating rod is connected with a second motor; and the telescopic assembly is arranged on the moving assembly, corresponds to the first rotating rod and the second rotating rod, and is electrically connected with the control element. The moving assembly drives the first rotating rod and the second rotating rod to move so as to be connected with the micropipette assemblies, the first motor drives the first rotating rod to rotate one of the micropipette assemblies, and the second motor drives the second rotating rod to rotate the other one of the micropipette assemblies.

Description

Technical Field

[0001] The present invention relates to a mobile device and a control method, and in particular to a mobile device and a control method thereof used in a nucleic acid extraction system. Background Art

[0002] Nucleic acid is one of the most basic substances of life and plays an important role in the growth, inheritance, and mutation of organisms. Therefore, society is still continuously investing in research on the structure, function, and use of nucleic acid molecules such as DNA and RNA, especially the application of nucleic acid molecules in the diagnosis and treatment of diseases and genetic engineering.

[0003] Generally, in the process of purifying nucleic acids by the mold tube method, the biological sample is first manually placed into the mold tube of the micro container, the micro container is manually placed in a centrifuge for centrifugation, the mold tube containing the nucleic acid is manually taken out, and the collection tube containing the waste liquid is manually taken out, which requires a lot of manpower and time.

[0004] In view of this, how to automate the process of nucleic acid purification by the mold tube method is one of the problems that urgently needs to be solved. Summary of the invention

[0005] The present invention provides a mobile device used in a nucleic acid extraction system and a control method thereof, which can convert the cumbersome, repetitive and boring processes of manually tightening a sealing cover, taking a micro-tube assembly, moving the micro-tube assembly, and removing the micro-tube assembly in the prior art into automation, thereby saving the labor of users.

[0006] The present invention provides a mobile device used in a nucleic acid extraction system, which cooperates with multiple micro-tube assemblies. The mobile device includes: a workbench; a mobile assembly, which is arranged on the workbench; a control element, which is arranged on the mobile assembly and electrically connected to the mobile assembly; a first motor, which is arranged on the mobile assembly and electrically connected to the control element; a first rotating rod, which is connected to the first motor; a second motor, which is arranged on the mobile assembly and electrically connected to the control element; a second rotating rod, which is connected to the second motor; and a telescopic assembly, which is arranged on the mobile assembly, and is correspondingly arranged with the first rotating rod and the second rotating rod, and is electrically connected to the control element, and can be moved between a first position and a second position; the mobile assembly drives the first rotating rod and the second rotating rod to move so as to engage with the micro-tube assemblies, the first motor drives the first rotating rod to rotate one of the micro-tube assemblies, and the second motor drives the second rotating rod to rotate the other of the micro-tube assemblies.

[0007] In some embodiments, the telescoping assembly moves from the first position to the second position so that the micropipette assembly is removed from the first rotating rod and the second rotating rod.

[0008] In some embodiments, the telescopic assembly further includes a driving motor and a telescopic member, and the telescopic member is connected to the driving motor.

[0009] In some embodiments, the telescopic member is disposed outside or inside the first rotating rod and the second rotating rod.

[0010] In some embodiments, the telescopic member has a plurality of sleeves, and the sleeves are sleeved on the outside of the first rotating rod and the second rotating rod.

[0011] In some embodiments, the moving component includes: a first moving member, which is disposed on a workbench; a second moving member, which is disposed on the first moving member and can be moved on the first moving member; a third moving member, which is disposed on the second moving member and can be moved on the second moving member; and a slider, which is disposed on the third moving member and can be moved on the third moving member.

[0012] In some embodiments, the moving direction of the second moving member, the moving direction of the third moving member, and the moving direction of the slider are perpendicular to each other.

[0013] The present invention provides a nucleic acid extraction system, which includes: a mobile device; and a centrifugal device, which is arranged corresponding to the mobile device and electrically connected to the mobile device, and includes: a body; a rotating motor, which is arranged on the body; a rotating disk, which is axially connected to the rotating motor; and a container base, which is pivotally connected to the rotating disk.

[0014] The present invention provides a control method for a mobile device used in a nucleic acid extraction system. The mobile device cooperates with a plurality of micro-tube assemblies. The control method comprises: a mobile assembly of the mobile device drives a first rotating rod and a second rotating rod to move so as to engage with the micro-tube assemblies; a first motor of the mobile device drives the first rotating rod to rotate one of the micro-tube assemblies in a first rotation direction, and a second motor of the mobile device drives the second rotating rod to rotate the other of the micro-tube assemblies in the first rotation direction; the mobile device drives the first rotating rod and the second rotating rod to move in a vertical direction; and the first motor drives the first rotating rod to rotate one of the micro-tube assemblies in a second rotation direction, and the second motor drives the second rotating rod to rotate the other of the micro-tube assemblies in the second rotation direction.

[0015] In some embodiments, the control method further includes: the moving component drives the first rotating rod and the second rotating rod to bring the multiple sealing caps and the multiple sample tubes of the micro-tube assembly away from the container base.

[0016] In some embodiments, the control method further includes: moving the telescopic assembly from the first position to the second position so that the micro-tube assembly is removed from the first rotating rod and the second rotating rod.

[0017] As mentioned above, the mobile device and control method thereof used in the nucleic acid extraction system of the present invention allow the user to place the container loaded with buffer on the buffer rack, put the sample into the sample tube of the micro-tube assembly, and then place the sample tube on the sample rack, and then the mobile device and control method thereof can be used to absorb the buffer, discharge the buffer to the sample tube loaded with the sample, tighten the sealing cover, take the micro-tube assembly, move the micro-tube assembly, remove the micro-tube assembly, and make the micro-tube assembly perform circular motion and other processes. In other words, the mobile device and control method thereof of the present invention can convert the cumbersome, repetitive and boring processes of the prior art, such as manually tightening the sealing cover, taking the micro-tube assembly, moving the micro-tube assembly, removing the micro-tube assembly, and making the micro-tube assembly perform circular motion, into automation, saving the labor of the user. And because the mobile device can process multiple micro-tube assemblies in batches, it is more efficient than the manual process of the prior art. BRIEF DESCRIPTION OF THE DRAWINGS

[0018] The details of one or more embodiments of the subject matter described in this specification are set forth in the following drawings and description. Other features, aspects, and advantages of the subject matter of this specification will become apparent from the description, drawings, and claims, including:

[0019] Figure 1 FIG. 4 is a perspective view of a mobile device according to an embodiment of the present invention.

[0020] Figure 2 A stereoscopic view of a microinjection tube assembly.

[0021] Figure 3A and Figure 3B It is a schematic diagram of the operation of the telescopic assembly of the present invention.

[0022] Figure 4 It is a three-dimensional diagram of an embodiment of the nucleic acid extraction system of the present invention.

[0023] Figure 5 It is a cross-sectional view of the centrifugal device of the nucleic acid extraction system of the present invention.

[0024] Figure 6 It is a three-dimensional view of the container base of the nucleic acid extraction system of the present invention.

[0025] Figure 7 A step diagram of a control method for a mobile device.

[0026] FIG. 8A to FIG. 8D Schematic diagram of the nucleic acid extraction system.

[0027] Wherein, the reference numerals are:

[0028] 10 Mobile Devices

[0029] 9 Microtube Assembly

[0030] 91 Sealing cover

[0031] 92 column container

[0032] 921 First protruding structure

[0033] 93 Collection pipe

[0034] 931 Matching Structure

[0035] 932 Second protruding structure

[0036] 94 Sample tubes

[0037] 11 Workbench

[0038] 111 Countertop

[0039] 112 Storage Rack

[0040] 1121 First groove structure

[0041] 113 Sample rack

[0042] 114 Buffer Rack

[0043] 12 Mobile Components

[0044] 121 First moving part

[0045] 122 Second moving part

[0046] 123 Third moving part

[0047] 124 Sliders

[0048] 1211 Linear track

[0049] 1212 Conveyor Belt

[0050] 1213 Track Chain

[0051] 13 Control elements

[0052] 14 Storage Components

[0053] 141 Preset operation instructions

[0054] 151 First Motor

[0055] 152 Second Motor

[0056] 153 First Rotating Rod

[0057] 154 Second Rotating Rod

[0058] 16 Telescopic components

[0059] 161 Drive motor

[0060] 162 Telescopic parts

[0061] 1621 Sleeve

[0062] 17 Liquid handling components

[0063] P1 First position

[0064] P2 Second position

[0065] D1 First direction

[0066] D2 Second direction

[0067] D3 third direction

[0068] 2 Nucleic Acid Extraction System

[0069] 20 Centrifugal device

[0070] 21 Ontology

[0071] 211 Chamber

[0072] 22 Rotary motor

[0073] 221 Rotating motor shaft

[0074] 23 Rotating disk

[0075] 231 Rotating disk axis

[0076] 24 Container Base

[0077] 241 Hole

[0078] 242 Second groove structure

[0079] 25 Heating module

[0080] 251 Heating Plate

[0081] 26 Cover

[0082] S1 First direction of rotation

[0083] S2 Second direction of rotation

[0084] 30. Control method of mobile device

[0085] S01~S04 Control method steps DETAILED DESCRIPTION

[0086] The detailed description and technical contents of the present invention are described as follows with reference to the accompanying drawings. However, the accompanying drawings are only provided for reference and description and are not intended to limit the present invention.

[0087] As used herein, terms such as "first", "second", etc. describe various elements, components, regions, layers and / or parts, which should not be limited by these terms. These terms can only be used to distinguish one element, component, region, layer or part from another. Unless the context clearly indicates, the terms such as "first", "second" used in this article do not imply a sequence or order.

[0088] Figure 1 FIG. 1 is a perspective view of a mobile device 10 according to an embodiment of the present invention. Figure 2 is a three-dimensional diagram of the micro-tube assembly 9. Please refer to Figure 1 and Figure 2 As shown, the mobile device 10 of this embodiment cooperates with the micro-tube assembly 9. The mobile device 10 of this embodiment includes a workbench 11, a mobile assembly 12, a control element 13, a first motor 151, a first rotating rod 153, a second motor 152, a second rotating rod 154 and a telescopic assembly 16.

[0089] The workbench 11 may have a table top 111. In some embodiments, the workbench 11 may further have a storage rack 112, a sample rack 113, and a buffer rack 114 disposed on the table top 111. The storage rack 112 is used to store sample tubes 94 loaded with nucleic acids, the sample rack 113 is used to store sample tubes 94 loaded with samples, and the buffer rack 114 is used to store containers loaded with buffer.

[0090] The moving assembly 12 is disposed on the workbench 11 . In this embodiment, the moving assembly 12 may include, for example, a first moving member 121 , a second moving member 122 , a third moving member 123 and a sliding block 124 .

[0091] The first moving member 121 is arranged on the table top 111 of the workbench 11. The first moving member 121 can be composed of, for example, a linear track body 1211, a conveyor belt 1212 and a track chain 1213, but it is not intended to limit the present invention. The track chain 1213 is used to protect and organize the wires to prevent the wires from falling off or getting entangled. In certain embodiments, the first moving member 121 can also utilize a linear track body to cooperate with a servo motor (not shown) and a threaded tube (not shown), the servo motor is arranged on the linear track body, and the threaded tube shaft is connected to the servo motor and the linear track body.

[0092] The second moving member 122 is disposed on the first moving member 121 and can move on the first moving member 121. The structure of the second moving member 122 is similar to that of the first moving member 121, and will not be described in detail. When the conveyor belt 1212 of the first moving member 121 moves, the second moving member 122 can be driven to move along the first direction D1 (for example, Figure 1The first movable member 121 moves in the left and right directions on the linear track body.

[0093] The third moving member 123 is disposed on the second moving member 122 and can move on the second moving member 122. The structure of the third moving member 123 is similar to that of the first moving member 121, and will not be described in detail herein. When the conveyor belt of the second moving member 122 moves, the third moving member 123 can be driven to move along the second direction D2 (for example, Figure 1 The second moving member 122 moves in the front-to-back direction (in the longitudinal direction) on the linear track body.

[0094] The slider 124 is disposed on the third moving member 123 and can move on the third moving member 123. When the conveyor belt of the third moving member 123 moves, the slider 124 can be driven to move along the third direction D3 (for example, Figure 1 The third movable member 123 moves in the vertical direction (in the vertical direction) on the linear track body.

[0095] Thus, the slider 124 can be moved in three-dimensional space by the first moving member 121, the second moving member 122 and the third moving member 123. In some embodiments, the moving directions of the second moving member 122, the third moving member 123 and the slider 124 are perpendicular to each other.

[0096] The control element 13 is disposed on the mobile component 12 and is electrically connected to the mobile component 12. The control element 13 may include, for example, a sensor integrated circuit, a microcontroller (MCU), a microprocessor (MPU), a central processing unit (CPU), an application-specific integrated circuit (ASIC), a digital signal processor (DSP), a graphics processing unit (GPU), a field programmable logic array (FPGA), or a system-on-chip (SoC), or other applicable chips, but they are not used to limit the present invention.

[0097] The first motor 151 is disposed on the moving component 12 and is electrically connected to the control element 13. In this embodiment, the first motor 151 is disposed on the slider 124 and the rotation axis of the first motor 151 is perpendicular to the table 111. In some embodiments, the first motor 151 is a servo motor or a stepping motor, but it is not intended to limit the present invention.

[0098] The first rotating rod 153 is connected to the first motor 151. In the present embodiment, the first rotating rod 153 is axially connected to the first motor 151 and rotates along the axis of the first motor 151. The material of the end of the first rotating rod 153 that contacts the micro-tube assembly 9 can be an elastic material, and the shape of the elastic material can be a straight line, a cross, a star, an explosion, or a polygon, but it is not intended to limit the present invention. The shape of the elastic material is set corresponding to the shape on the sealing cover 91 of the micro-tube assembly 9. When the first rotating rod 153 is connected to the sealing cover 91, a mechanical interference is generated, so that the sealing cover 91 is driven by the first rotating rod 153, but it is not intended to limit the present invention.

[0099] The second motor 152 is disposed on the moving assembly 12 and is electrically connected to the control element 13. In this embodiment, the second motor 152 is disposed on the slider 124 and the rotation axis of the second motor 152 is perpendicular to the table 111. The second motor 152 is disposed side by side with the first motor 151. The second motor 152 is a servo motor or a stepping motor, but it is not intended to limit the present invention.

[0100] The second rotating rod 154 is connected to the second motor 152. In the present embodiment, the second rotating rod 154 is axially connected to the second motor 152 and rotates along the axis of the second motor 152. The second rotating rod 154 is similar to the first rotating rod 153, and the material of the end contacting the micro-tube assembly 9 can be an elastic material, and the shape of the elastic material can be a straight line, a cross, a star, an explosion, or a polygon, but it is not used to limit the present invention. The shape of the elastic material is set corresponding to the shape on the sealing cover 91 of the micro-tube assembly 9. When the second rotating rod 154 is connected to the sealing cover 91, a mechanism interference is generated, so that the sealing cover 91 is driven by the second rotating rod 154.

[0101] In some embodiments, the mobile device 10 may also have a third motor and a third rotating rod. The third motor is disposed on the mobile component 12 and is electrically connected to the control element 13. The third rotating rod is connected to the third motor. Of course, the mobile device 10 may also have a fourth motor and a fourth rotating rod, which will not be described in detail.

[0102] In some embodiments, the micro-tube assembly 9 that cooperates with the mobile device 10 may, for example, have a sealing cover 91, a column container 92, a liquid collecting tube 93, and a sample tube 94. It is worth mentioning that the mobile device 10 can cooperate with multiple micro-tube assemblies 9. For example, if the mobile device 10 has two rotating rods, it can cooperate with two micro-tube assemblies 9, and if the mobile device 10 has four rotating rods, it can cooperate with four micro-tube assemblies 9, and so on. The column container 92 has a first protruding structure 921, and the liquid collecting tube 93 has a matching structure 931 and a second protruding structure 932. The matching structure 931 is a concave structure, and the matching structure 931 corresponds to the first protruding structure 921. Therefore, when the column container 92 is placed in the accommodating space of the liquid collecting tube 93, the first protruding structure 921 can be embedded in the matching structure 931 from top to bottom. The sample tube 94 has a similar structure to the liquid collecting tube 93, and will not be repeated here. In some embodiments, the capacity of the column container 92 is smaller than the capacity of the sample tube 94 and smaller than the capacity of the collection tube 93 .

[0103] The storage rack 112 of the workbench 11 may have a first groove structure 1121 corresponding to the number and shape of the second protrusion structure 932. Therefore, when the liquid collecting tube 93 is placed in the accommodation space of the storage rack 112, the second protrusion structure 932 can be embedded in the first groove structure 1121 from top to bottom. Therefore, the side wall of the second protrusion structure 932 of the liquid collecting tube 93 abuts against the side wall of the first groove structure 1121 of the storage rack 112, so that the liquid collecting tube 93 cannot rotate. The sample rack 113 is similar to the storage rack 112 in structure, and will not be described in detail here.

[0104] Figure 3A and Figure 3B This is a schematic diagram of the operation of the telescopic assembly 16 of the present invention. Figure 1 , Figure 2 , Figure 3A and Figure 3B As shown, the telescopic assembly 16 is disposed on the moving assembly 12 , corresponding to the first rotating rod 153 and the second rotating rod 154 , and is electrically connected to the control element 13 , and can be moved between a first position P1 and a second position P2 .

[0105] In this embodiment, the telescopic assembly 16 is disposed on the slider 124, and the telescopic assembly 16 further includes a driving motor 161 and a telescopic member 162, the telescopic member 162 is connected to the driving motor 161, and the driving motor 161 is electrically connected to the control element 13. The telescopic member 162 can be disposed outside or inside the first rotating rod 153 and the second rotating rod 154. In this embodiment, the telescopic member 162 has a sleeve 1621, and the sleeve 1621 is sleeved outside the first rotating rod 153 and the second rotating rod 154. The telescopic member 162 does not contact the first rotating rod 153 and the second rotating rod 154.

[0106] The telescopic assembly 16 can be moved from the first position P1 to the second position P2, so that the micro-tube assembly 9 is removed from the first rotating rod 153 and the second rotating rod 154. When the driving motor 161 rotates, the sleeve 1621 is driven to move from the first position P1 to the second position P2, and at this time, the sleeve 1621 protrudes from the first rotating rod 153 and the second rotating rod 154, so that the micro-tube assembly 9 driven by the first rotating rod 153 and the second rotating rod 154 is removed. In other embodiments, the sleeve 1621 can also be sleeved inside the first rotating rod 153 and the second rotating rod 154.

[0107] In some embodiments, the mobile device 10 may further include a storage element 14 (eg Figure 1 14 is electrically connected to the control element 13. The storage element 14, for example, includes a preset operation instruction 141. The control element 13 controls the mobile device 10 according to the preset operation instruction 141, for example, controls the mobile component 12 to drive the first rotating rod 153 and the second rotating rod 154 to move to engage with the micro-tube assembly 9, the first motor 151 drives the first rotating rod 153 to rotate one of the micro-tube assemblies 9, and the second motor 152 drives the second rotating rod 154 to rotate the other of the micro-tube assembly 9. Or, for example, controls the first moving member 121, the second moving member 122 and the third moving member 123 so that the slider 124 can be driven by the first moving member 121, the second moving member 122 and the third moving member 123 in three-dimensional space. Or, for example, controls the first motor 151 and the second motor 152 to drive the first rotating rod 153 and the second rotating rod 154. Or, for example, controls the drive motor 161 so that the sleeve 1621 is driven to move from the first position P1 to the second position P2. However, it is not used to limit the present invention.

[0108] Figure 4 This is a perspective view of an embodiment of the nucleic acid extraction system 2 of the present invention. Figure 4 As shown, in some embodiments, the mobile device 10 may further include a liquid transfer component 17 disposed on the third moving member 123. The liquid transfer component 17 may include, for example, a straw 171, which can suck and discharge liquid.

[0109] As described above, the mobile device 10 of this embodiment cooperates with the micro-tube assembly 9, so that the user only needs to place the container loaded with buffer on the buffer rack 114, put the sample into the sample tube 94 of the micro-tube assembly 9, and then place the sample tube 94 on the sample rack 113, and then the mobile assembly 12 of the mobile device 10 can be used to absorb the buffer, discharge the buffer to the sample tube 94 loaded with the sample, tighten the sealing cover 91, take the micro-tube assembly 9, move the micro-tube assembly 9, and remove the micro-tube assembly 9. Since the mobile device 10 of this embodiment can have more than two rotating motors and more than two rotating rods, the mobile device 10 can process more than two micro-tube assemblies 9 at a time. In some embodiments, the mobile device 10 of this embodiment can process four micro-tube assemblies 9 at a time through the third motor, the third rotating rod, the fourth motor, and the fourth rotating rod, and the efficiency is multiplied. In other words, the mobile device 10 of this embodiment can convert the cumbersome, repetitive and boring processes of manually tightening the sealing cover 91, taking the micro-tube assembly 9, moving the micro-tube assembly 9, and removing the micro-tube assembly 9 in the prior art into automation, saving the labor of the user. And because the mobile device 10 can process multiple micro-tube assemblies 9 in batches, it is more efficient than the manual process of the prior art. In addition, compared with the existing automated machines on the market, due to technical bottlenecks, it is impossible to operate multiple mold tubes at the same time, where the mold tubes can be, for example, micro-tube assemblies 9. The mobile device 10 of this embodiment can process multiple micro-tube assemblies 9 in batches, and the efficiency is multiplied.

[0110] Figure 4 It is a three-dimensional diagram of an embodiment of the nucleic acid extraction system 2 of the present invention. Figure 5 This is a cross-sectional view of the centrifugal device 20 of the nucleic acid extraction system 2 of the present invention. Figure 4 and Figure 5 As shown, the nucleic acid extraction system 2 of this embodiment can be used with Figure 1 The mobile device 10 is used in conjunction with the mobile device 10, but this is not limiting. Of course, the mobile device can also use other mobile devices different from the mobile device 10. The nucleic acid extraction system 2 of this embodiment includes the mobile device 10 and the centrifugal device 20. The mobile device 10 can be used, for example, as Figure 1 , 3A In addition, in some embodiments, the mobile device 10 may further include a liquid transfer component 17 disposed on the third moving member 123. The liquid transfer component 17 may include a straw 171, which can absorb and discharge liquid.

[0111] The centrifugal device 20 is used to extract nucleic acid from biological samples and may include a body 21 , a rotary motor 22 , a rotary disk 23 and a container base 24 .

[0112] In this embodiment, the body 21 may include, for example, a chamber 211 . The rotary motor 22 , the rotary disk 23 and the container base 24 are disposed in the chamber 211 .

[0113] The rotary motor 22 is disposed on the body 21 . The rotary motor 22 may be completely disposed in the chamber 211 , or only the rotary motor shaft 221 of the rotary motor 22 may be disposed in the chamber 211 .

[0114] In this embodiment, the rotating disk 23 has a rotating disk axis 231, which is axially connected to the rotating motor axis 221. In this embodiment, when the rotating motor 22 is activated, the rotating motor 22 can drive the rotating disk 23 to rotate horizontally in the chamber 211 around the rotating motor axis 221.

[0115] Figure 6 This is a three-dimensional diagram of the container base 24 of the nucleic acid extraction system of the present invention. Figure 2 , Figure 4 and Figure 6 As shown, the container base 24 is pivotally connected to the rotating disk 23. In this embodiment, the container base 24 has a hole 241 and a second groove structure 242. The number and shape of the second groove structure 242 correspond to the second protrusion structure 932 of the liquid collecting tube 93. Therefore, when the liquid collecting tube 93 is placed in the accommodation space of the hole 241, the second protrusion structure 932 can be embedded in the second groove structure 242 from top to bottom. Therefore, the side wall of the second protrusion structure 932 of the liquid collecting tube 93 abuts against the side wall of the second groove structure 242 of the container base 24, so that the liquid collecting tube 93 cannot rotate.

[0116] Please refer to Figure 2 , Figure 4 and Figure 5 As shown, when the rotary motor 22 drives the rotating disk 23 to rotate, the container base 24 and the micro-tube assembly 9 disposed in the container base 24 will be driven by the rotating disk 23 and perform a circular motion in the chamber 211 along the rotating motor axis 221 as the center.

[0117] In some embodiments, the centrifugal device 20 may further include a cover 26 corresponding to the body 21. Before the rotary motor 22 is started, the cover 26 may be closed on the chamber 211 to prevent the user's hand from accidentally reaching into the chamber 211 and colliding with the rotating disk 23, the container base 24 or the micro-tube assembly 9 after the rotary motor 22 is started, causing injury.

[0118] In some embodiments, the centrifugal device 20 may further include a heating module 25 disposed on the body 21. The heating module 25 is disposed inside the body 21 and is used to heat the chamber 211 to the operating environment temperature. In some embodiments, the heating module 25 may have a heating plate 251 and is attached to the inner surface of the cover 26. It should be noted that the shape and location of the heating module 25 are not limited thereto, and the heating module 25 may also be a heating rod or other device with a heating function, and the heater may also be disposed at other locations inside the body 21, and will not interfere with the rotary motor 22, the rotating disk 23 and the container base 24.

[0119] As described above, the nucleic acid extraction system 2 of the present embodiment can cooperate with the micro-tube assembly 9, so that the user only needs to place the container loaded with buffer on the buffer rack 114, put the sample into the sample tube 94 of the micro-tube assembly 9, and then place the sample tube 94 on the sample rack 113, and then the first motor 151, the first rotating rod 153, the second motor 152, the second rotating rod 154, the telescopic assembly 16 and the pipetting assembly 17 of the moving assembly 12 of the moving device 10 can be used to absorb the buffer, discharge the buffer to the sample tube 94 loaded with the sample, tighten the sealing cover 91, take the micro-tube assembly 9, move the micro-tube assembly 9, remove the micro-tube assembly 9, and make the micro-tube assembly 9 perform circular motion. Since the moving device 10 has more than two rotating motors and more than two rotating rods, the moving device 10 can process more than two micro-tube assemblies 9 at a time. In some embodiments, the moving device 10 can have a third motor, a third rotating rod, a fourth motor, and a fourth rotating rod to process four micro-tube assemblies 9 at a time, and the efficiency is multiplied. In other words, the nucleic acid extraction system 2 of the present embodiment can convert the cumbersome, repetitive and boring processes of the prior art, such as manually tightening the sealing cover 91, taking the micro-tube assembly 9, moving the micro-tube assembly 9, removing the micro-tube assembly 9, and making the micro-tube assembly 9 perform circular motion, into automation, saving the labor of the user. And because the mobile device 10 can process multiple micro-tube assemblies 9 in batches, it is more efficient than the manual process of the prior art. In addition, compared with the existing automated machines on the market, due to technical bottlenecks, it is impossible to operate multiple mold tubes at the same time, where the mold tubes can be, for example, micro-tube assemblies 9. The nucleic acid extraction system 2 of the present embodiment can process multiple micro-tube assemblies 9 in batches, and the efficiency is multiplied.

[0120] In addition, the centrifuge device 20 of the nucleic acid extraction system 2 of the present embodiment can extract nucleic acid from the sample in the micro-tube assembly 9. Furthermore, the heating module 25 can directly heat and dry the liquid on the filter membrane of the centrifuge tube after the centrifuge device 20 completes the centrifugation, without the need for additional high-speed operation or moving to other heating equipment, thereby reducing process time and equipment costs.

[0121] Figure 7FIG. 3 is a step diagram of a control method 30 for a mobile device. Figure 1 The mobile device 10 is not intended to be limiting. Figure 7 As shown, step S01 is that the moving component of the moving device drives the first rotating rod and the second rotating rod to move to engage with multiple micro-tube assemblies. Step S02 is that the first motor of the moving device drives the first rotating rod to rotate one of the micro-tube assemblies in the first rotation direction, and the second motor of the moving device drives the second rotating rod to rotate the other of the micro-tube assemblies in the first rotation direction. Step S03 is that the moving device drives the first rotating rod and the second rotating rod to move in a vertical direction. Step S04 is that the first motor drives the first rotating rod to rotate one of the micro-tube assemblies in the second rotation direction, and the second motor drives the second rotating rod to rotate the other of the micro-tube assemblies in the second rotation direction.

[0122] Figure 1 , Figure 2 , Figure 4 , Figure 5 and Figure 7 As shown, in some embodiments, before step S01, the control element 13 of the mobile device 10 can control the pipetting component 17 to first absorb the buffer solution, and then discharge the buffer solution into the sample tube 94 on the sample rack 113. In some embodiments, the sample rack 113 can be shaken and heated, but it is not used to limit the present invention. The control element 13 then controls the pipetting component 17 to absorb the sample and buffer solution in the sample tube 94, and then controls the pipetting component 17 to discharge the sample and buffer solution into the column container 92 in the liquid collecting tube 93 in the container base 24. In other words, the column container 92 is sleeved in the liquid collecting tube 93. The control element 13 controls the cover 26 of the centrifugal device 20 to be closed. Thereby, after the sample and the buffer solution are mixed, they are placed in the centrifugal device 20 to prepare for centrifugation.

[0123] The control element 13 controls the rotating motor 22 of the centrifugal device 20 to drive the rotating disk 23 of the centrifugal device 20 to make the micro-tube assembly 9 perform circular motion. After the centrifugation operation is completed, the control element 13 controls the centrifugal device 20 to stop the rotating motor 22. The control element 13 controls the cover 26 of the centrifugal device 20 to open. Thereby, a centrifugation operation is performed to extract nucleic acid.

[0124] In some embodiments, if only one centrifugation operation is performed, impurities other than nucleic acid will still remain on the column container 92, so the above steps of adding buffer and centrifugation can be repeated two to three times, so that purer nucleic acid is left on the column container 92, and impurities are loaded into the liquid collection tube 93. In this way, multiple centrifugation operations are performed to extract nucleic acid.

[0125] The control element 13 of the mobile device 10 can control the first moving member 121, the second moving member 122 and the third moving member 123 of the moving assembly 12 to drive the slider 124, the first rotating rod 153 and the second rotating rod 154, and control the first motor 151 and the second motor 152 to rotate, so as to drive the first rotating rod 153 and the second rotating rod 154 to rotate together, so that the first rotating rod 153 and the second rotating rod 154 are engaged with the sealing cover 91 and generate mechanical interference. In this way, the mobile device 10 grabs the sealing cover 91.

[0126] FIG. 8A to FIG. 8D FIG. 2 is a schematic diagram of the operation of the nucleic acid extraction system 2. Figure 1 , Figure 2 , Figure 7 , Fig. 8A and Figure 8B As shown, the moving assembly 12 drives the first rotating rod 153 and the second rotating rod 154 to the container base 24 of the centrifugal device 20. In other words, the control element 13 can control the moving assembly 12 to move the sealing cover 91 fixed to the first rotating rod 153 and the second rotating rod 154 to the column container 92 after the centrifugation operation is completed. In this way, the moving device 10 moves to the centrifugal device 20.

[0127] In step S01, the moving assembly 12 of the moving device 10 drives the first rotating rod 153 and the second rotating rod 154 to move to engage with the micro-tube assembly 9. In this embodiment, the moving assembly 12 of the moving device 10 can drive the first rotating rod 153, the second rotating rod 154 and the sealing cover 91 to the centrifugal device 20, thereby engaging with the column container 92 of the micro-tube assembly 9.

[0128] like Figure 1 , Figure 2 , Figure 7 and Figure 8C As shown, in step S02, the first motor 151 of the mobile device 10 drives the first rotating rod 153 to rotate one of the micro-tube assemblies 9 in the first rotating direction S1, and the second motor 152 of the mobile device 10 drives the second rotating rod 154 to rotate the other of the micro-tube assemblies 9 in the first rotating direction S1. In this embodiment, the first rotating direction S1 is the direction for tightening the sealing cover 91 to the column container 92. When the first motor 151 and the second motor 152 rotate along the first rotating direction S1, the first rotating rod 153 and the second rotating rod 154 are driven to rotate along the first rotating direction S1, and the sealing cover 91 is driven to rotate along the first rotating direction S1, and the column container 92 does not rotate accordingly. In this way, the sealing cover 91 can be tightened on the column container 92.

[0129] In step S03, the moving device 10 drives the first rotating rod 153 and the second rotating rod 154 to move in a vertical direction. In this embodiment, when the first rotating rod 153 and the second rotating rod 154 rotate the sealing cover 91 in the same first rotating direction S1, the first protruding structure of the column container 92 applies an external torque to the liquid collecting tube 93. Since the liquid collecting tubes 93 are connected side by side, the liquid collecting tubes 93 are twisted and deformed, and the liquid collecting tubes 93 and the column container 92 above them have mechanical interference. Therefore, when the first rotating rod 153 and the second rotating rod 154 move in a vertically upward direction, the sealing cover 91, the column container 92 and the liquid collecting tube 93 are lifted up at the same time.

[0130] like Figure 1 , Figure 2 , Figure 7 and Fig.8D As shown, in step S04, the first motor 151 drives the first rotating rod 153 to rotate one of the micro-tube assemblies 9 in the second rotating direction S2, and the second motor 152 drives the second rotating rod 154 to rotate the other of the micro-tube assemblies 9 in the second rotating direction S2. In this embodiment, the second rotating direction S2 is the direction to loosen the sealing cover 91 from the column container 92. The first motor 151 and the second motor 152 rotate along the second rotating direction S2, and the first rotating rod 153 and the second rotating rod 154 are driven to rotate along the second rotating direction S2, so that the collection tube 93 is freed from the external torque caused by the tightening of the column container 92 and the distortion. In this way, the collection tube 93 and the column container 92 above it are released from the interference mechanism, and the collection tube 93 is thus loosened from the column container 92.

[0131] like Figure 1 , Figure 2 , Figure 4 and Fig.8D As shown, in some embodiments, the moving device 10 drives the first rotating rod 153, the second rotating rod 154 and the column container 92 to the sample tube 94 in the container base 24 of the centrifugal device 20, and the first motor 151 and the second motor 152 rotate along the second rotation direction S2 to loosen the column container 92 and the sealing cover 91, and place the column container 92 on the sample tube 94. Then, the buffer in the container of the loading buffer, such as pure water, is sucked through the pipetting component 17 and discharged into the column container 92. Then, a centrifugal operation similar to the above is performed, which will not be repeated here. In this way, the nucleic acid is loaded on the sample tube 94.

[0132] The moving device 10 tightens the sealing cover 91 onto the column container 92, and then drives the first rotating rod 153 and the second rotating rod 154 to drive the column container 92 to the liquid collecting tube 93 in the container base 24 of the centrifugal device 20. The first motor 151 and the second motor 152 rotate along the second rotation direction S2 to loosen the column container 92 and the sealing cover 91, and place the column container 92 on the liquid collecting tube 93. The moving device 10 then tightens the sealing cover 91 onto the sample tube 94. In this way, the sample tube 94 loaded with nucleic acid is driven by the moving device 10.

[0133] like Figure 1 , Figure 2 , Figure 3A , Figure 3B , Fig. 8A and Figure 8B As shown, the moving assembly 12 drives the first rotating rod 153 and the second rotating rod 154 to take the sealing cover 91 and the sample tube 94 of the micro-tube assembly 9 away from the container base 24 and move them to the storage rack 112. The telescopic assembly 16 moves from the first position P1 to the second position P2, so that the micro-tube assembly 9 is removed from the first rotating rod 153 and the second rotating rod 154. In this embodiment, the telescopic assembly 16 protrudes from the first rotating rod 153 and the second rotating rod 154, so that the sealing cover 91 and the sample tube 94 driven by the first rotating rod 153 and the second rotating rod 154 are removed to the storage rack 112. In this way, the sample tube 94 loaded with nucleic acid is stored in the storage rack 112.

[0134] In summary, the mobile device and control method thereof used in the nucleic acid extraction system of the present invention allow the user to place the container loaded with buffer on the buffer rack, place the sample into the sample tube of the micro-tube assembly, and then place the sample tube on the sample rack, and then the nucleic acid extraction system can be used to absorb the buffer, discharge the buffer to the sample tube loaded with the sample, tighten the sealing cover, take the micro-tube assembly, move the micro-tube assembly, remove the micro-tube assembly, and make the micro-tube assembly perform circular motion and other processes. In other words, the mobile device, nucleic acid extraction system and control method thereof of the present invention can convert the cumbersome, repetitive and boring processes of the prior art, such as manually tightening the sealing cover, taking the micro-tube assembly, moving the micro-tube assembly, removing the micro-tube assembly, and making the micro-tube assembly perform circular motion, into automation, saving the user's labor. And because the mobile device can process multiple micro-tube assemblies in batches, it is more efficient than the manual process of the prior art.

[0135] The components of several embodiments are summarized above so that those with ordinary knowledge in the technical field to which the present invention belongs can better understand the concept of the embodiments of the present invention. Those with ordinary knowledge in the technical field to which the present invention belongs should understand that the embodiments of the present invention can be used as a basis to design or modify other processes and structures to achieve the same purpose and / or achieve the same benefits as the embodiments introduced herein. Those with ordinary knowledge in the technical field to which the present invention belongs should also understand that these equivalent structures do not deviate from the spirit and scope of the present invention, and various changes, substitutions and other options can be made here without deviating from the spirit and scope of the present invention. Therefore, the scope of protection of the present invention shall be defined as defined by the appended claims.

Claims

1. A mobile device used in a nucleic acid extraction system, characterized in that: In conjunction with a plurality of micro-tube assemblies, the moving device comprises: A workbench; A moving assembly is disposed on the workbench; a control element, disposed on the moving component and electrically connected to the moving component; a first motor, disposed on the moving component and electrically connected to the control element; a first rotating rod connected to the first motor; a second motor, disposed on the moving component and electrically connected to the control element; a second rotating rod connected to the second motor; and A telescopic assembly is disposed on the moving assembly, is disposed corresponding to the first rotating rod and the second rotating rod, is electrically connected to the control element, and is movable between a first position and a second position; The moving assembly drives the first rotating rod and the second rotating rod to move and engage with the micro-tube assemblies. The first motor drives the first rotating rod to rotate one of the micro-tube assemblies, and the second motor drives the second rotating rod to rotate another of the micro-tube assemblies.

2. The mobile device according to claim 1, wherein: The telescopic assembly moves from the first position to the second position so that the micro-tube assemblies are removed from the first rotating rod and the second rotating rod.

3. The mobile device according to claim 1, wherein: The telescopic assembly further includes a driving motor and a telescopic member, and the telescopic member is connected to the driving motor.

4. The mobile device according to claim 3, wherein: The telescopic member is arranged outside or inside the first rotating rod and the second rotating rod.

5. The mobile device according to claim 4, wherein: The telescopic member has a plurality of sleeves, and the sleeves are sleeved on the outside of the first rotating rod and the second rotating rod.

6. The mobile device according to claim 1, wherein: The mobile component contains: A first moving member is disposed on the workbench; a second moving member, disposed on the first moving member and movable on the first moving member; a third moving member, disposed on the second moving member and movable on the second moving member; and A sliding block is arranged on the third moving member and can move on the third moving member.

7. The mobile device according to claim 6, wherein: The moving direction of the second moving member, the moving direction of the third moving member and the moving direction of the sliding block are perpendicular to each other.

8. A nucleic acid extraction system, characterized in that: Include: The mobile device according to any one of claims 1 to 7; and A centrifugal device is disposed corresponding to the mobile device and is electrically connected to the mobile device, comprising: one body; A rotary motor is disposed on the body; a rotating disk, axially connected to the rotating motor; and A container base is pivotally connected to the rotating disk.

9. A control method for a mobile device used in a nucleic acid extraction system, characterized in that: The moving device cooperates with a plurality of micro-tube assemblies, and the control method includes: A moving component of the moving device drives a first rotating rod and a second rotating rod to move so as to engage with the micro-tube components; A first motor of the moving device drives the first rotating rod to rotate one of the micro-tube assemblies in a first rotation direction, and a second motor of the moving device drives the second rotating rod to rotate another of the micro-tube assemblies in the first rotation direction; The moving device drives the first rotating rod and the second rotating rod to move in a vertical direction; and The first motor drives the first rotating rod to rotate the one of the micro-tube assemblies in a second rotating direction, and the second motor drives the second rotating rod to rotate the other of the micro-tube assemblies in the second rotating direction.

10. The control method according to claim 9, characterized in that: Also includes: The moving assembly drives the first rotating rod and the second rotating rod to take the sealing covers and the sample tubes of the micro-tube assemblies away from a container base.

11. The control method according to claim 10, characterized in that: Also includes: A telescopic assembly moves from a first position to a second position so that the micro-tube assemblies are removed from the first rotating rod and the second rotating rod.

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