Planting method for improving germination rate of purple perilla in alpine region

Abstract

A planting method for increasing the germination rate of purple perilla in alpine regions belongs to the technical field of plant treatment. The method comprises the following steps: mixing bacillus fermentation liquor with an adsorbent according to a mass ratio of 1: 2 to prepare a bacillus adsorbent; 5 mL of a 4% carboxymethyl cellulose sodium adhesive is added into 15 g of a bacillus adsorbent, 100 g of purple perilla seeds soaked in the melatonin solution obtained in the third step for 4 h are added and stirred repeatedly so that all the purple perilla seeds can be adsorbed by the bacillus adsorbent, 30 g of bentonite is added and stirred evenly, at the moment, each seed is coated with a layer of red coating material, and the purple perilla seeds are obtained. And placing the coated purple perilla seeds in a shady, cool, dry and ventilated place, and drying in the shade for later use. According to the method, the situation that perilla seeds in spring are dug by birds such as sparrows or pigeons is reduced, the number of the seeds in soil is increased, the stress resistance of perilla is improved, and the content of alpha-linolenic acid in perilla is increased.

Description

Technical Field

[0001] The invention belongs to the technical field of plant treatment, and in particular relates to a planting method for improving the germination rate of perilla in high-cold areas. Background Art

[0002] Heilongjiang Province is located in a high-altitude and cold area. The winter climate is cold for a long time, and the spring is windy, dry and with little rain. It is difficult for perilla seeds to physically swell. Secondly, there is a shortage of food for sparrows, pigeons and other birds in early May. The unique fragrance of perilla seeds will attract them to dig for seeds in the fields, resulting in a decrease in the number of seeds in the soil, a low field germination rate, uneven germination, or even if germination occurs, the seedlings will be weak and easily cause seedling withering and death, which brings many inconveniences to standardized cultivation and even affects the improvement of economic benefits. It is imperative to study and develop a perilla seed germination method with strong stress resistance in high-altitude and cold areas. Summary of the invention

[0003] The invention aims to solve the problem that birds in high-altitude cold areas eat perilla seeds in spring, resulting in a reduction in the number of seeds, and to provide a planting method for improving the germination rate of perilla in high-altitude cold areas.

[0004] To achieve the above purpose, the technical solution adopted by the present invention is as follows:

[0005] A planting method for improving the germination rate of Perilla in high-cold areas, the method comprising:

[0006] Step 1: Select perilla seeds: Choose perilla seeds with full grains, uniform size and high maturity;

[0007] Step 2: The Bacillus strain purchased from the market was streaked on TSA solid medium and placed in an incubator at 28-30°C in the dark. After the colonies with an average diameter of more than 1 mm visible to the naked eye grew, they were inoculated on TSA liquid medium and placed on a shaker at 32-38°C and 170-190 rpm in the dark. After 5 days, when the culture solution became turbid, the shaking culture was stopped to obtain a concentration of 1×10 9 cfu·mL -1 The Bacillus fermentation liquid is cooled naturally and set aside;

[0008] Step 3: Prepare 90-110 μmol / L melatonin solution, place in a brown bottle, and store in a 4°C refrigerator for later use;

[0009] Step 4: Preparation process of perilla seed adsorbent formula: 50%-60% roasted tobacco powder, 16%-24% hemp leaf powder, 16%-24% flax leaf powder, 4.1% Ca(H2PO4), 0.2% sucrose, 0.8% lime powder, 0.2% capsanthin; add 1mL 0.5vol.% NaMoO4 and 1mL 0.5vol.% H3BO3 to every 500g of the above raw materials, stir well and set aside;

[0010] Step 5: Bacillus fermentation liquid and adsorbent are mixed in a mass ratio of 1:2 to prepare Bacillus adsorbent; 5 mL of 4% sodium hydroxymethylcellulose adhesive is added to 15 g of Bacillus adsorbent, and 100 g of perilla seeds soaked in the melatonin solution obtained in step 3 for 4 hours are added, and the mixture is stirred repeatedly so that the perilla seeds are all adsorbed by the Bacillus adsorbent, and 30 g of bentonite is added and stirred evenly. At this time, each seed has a layer of red coating material. The coated perilla seeds are placed in a cool, dry and ventilated place and dried in the shade for use.

[0011] A planting method for improving the germination rate of Perilla in high-cold areas, the method comprising:

[0012] Step 1: Select perilla seeds: Choose perilla seeds with full grains, uniform size and high maturity;

[0013] Step 2: The Bacillus strain purchased from the market was streaked on TSA solid medium and placed in an incubator at 28-30°C in the dark. After the colonies with an average diameter of more than 1 mm visible to the naked eye grew, they were inoculated on TSA liquid medium and placed on a shaker at 32-38°C and 170-190 rpm in the dark. After 5 days, when the culture solution became turbid, the shaking culture was stopped to obtain a concentration of 1×10 9 cfu·mL -1 The Bacillus fermentation liquid is cooled naturally and set aside;

[0014] Step 3: Prepare 90-110 μmol / L melatonin solution, place in a brown bottle, and store in a 4°C refrigerator for later use;

[0015] Step 4: Preparation process of perilla seed adsorbent formula: 50%-60% roasted tobacco powder, 16%-24% hemp leaf powder, 16%-24% flax leaf powder, 4.1% Ca(H2PO4), 0.2% sucrose, 1% lime powder; add 1mL 0.5vol.% NaMoO4 and 1mL 0.5vol.% H3BO3 to every 500g of the above raw materials, stir well and set aside;

[0016] Step 5: Bacillus fermentation liquid and adsorbent are mixed in a mass ratio of 1:2 to prepare Bacillus adsorbent; 5 mL of 4% sodium hydroxymethylcellulose adhesive is added to 15 g of Bacillus adsorbent, and 100 g of perilla seeds soaked in the melatonin solution obtained in step 3 for 4 hours are added, and the mixture is stirred repeatedly so that the perilla seeds are all adsorbed by the Bacillus adsorbent, and 30 g of bentonite is added and stirred evenly. At this time, each seed has a layer of brown coating material. The coated perilla seeds are placed in a cool, dry and ventilated place and dried in the shade for use.

[0017] A planting method for improving the germination rate of Perilla in high-cold areas, the method comprising:

[0018] Step 1: Select perilla seeds: Choose perilla seeds with full grains, uniform size and high maturity;

[0019] Step 2: The Bacillus strain purchased from the market was streaked on TSA solid medium and placed in an incubator at 28-30°C in the dark. After the colonies with an average diameter of more than 1 mm visible to the naked eye grew, they were inoculated on TSA liquid medium and placed on a shaker at 32-38°C and 170-190 rpm in the dark. After 5 days, when the culture solution became turbid, the shaking culture was stopped to obtain a concentration of 1×10 9 cfu·mL -1 The Bacillus fermentation liquid is cooled naturally and set aside;

[0020] Step 3: Prepare 90-110 μmol / L melatonin solution, place in a brown bottle, and store in a 4°C refrigerator for later use;

[0021] Step 4: Preparation process of perilla seed adsorbent formula: peat soil 94%-96%, Ca(H2PO4) 2.8%-4.7%, sucrose 0.2%, lime powder 1%; add 1mL 0.5vol.% NaMoO4 and 1mL 0.5vol.% H3BO3 to every 500g of the above raw materials, stir well and set aside;

[0022] Step 5: Bacillus fermentation liquid and adsorbent are mixed in a mass ratio of 1:2 to prepare Bacillus adsorbent; 5 mL of 4% sodium hydroxymethylcellulose adhesive is added to 15 g of Bacillus adsorbent, and 100 g of perilla seeds soaked in the melatonin solution obtained in step 3 for 4 hours are added, and the mixture is stirred repeatedly so that the perilla seeds are all adsorbed by the Bacillus adsorbent, and 30 g of bentonite is added and stirred evenly. At this time, each seed has a layer of brown coating material. The coated perilla seeds are placed in a cool, dry and ventilated place and dried in the shade for use.

[0023] The beneficial effects of the present invention compared to the prior art are:

[0024] (1) Reduce the risk of perilla seeds being eaten by birds such as sparrows and pigeons in spring, increase the number of seeds in the soil, improve the stress resistance of perilla, and increase the content of α-linolenic acid in perilla.

[0025] (2) Red seeds can make birds such as sparrows and pigeons feel irritated and feel that there is a danger. The present invention dyes the perilla seeds red, which can reduce the consumption of birds such as sparrows and pigeons and increase the number of seeds in the field.

[0026] (3) Flue-cured tobacco leaf powder contains nicotine and nicotine, which have a certain killing effect on the eggs and larvae of underground pests when they enter the soil. At the same time, the potassium content is relatively high, generally above 4%, which can make the root system of Perilla more developed, improve the photosynthesis and photosynthetic intensity of Perilla, enhance the activity of enzymes in the Perilla body, promote various metabolic activities in the body, and play an important role in promoting the growth and development of the stems and leaves of Perilla and the formation of seeds.

[0027] (4) Flue-cured tobacco leaf powder, hemp leaf powder and flax leaf powder can improve the diversity of microorganisms in the soil, reduce the impact of soil-borne pests and diseases on Perilla frutescens, and combine with the aphid-repelling and antibacterial effects of Perilla frutescens to improve the yield and quality of Perilla frutescens leaves and seeds.

[0028] (5) Melatonin can be used in the cultivation of perilla seedlings to increase the height of perilla plants, promote the growth of perilla branches, increase the thickness of perilla stems, increase the area of ​​perilla leaves, enhance the photosynthetic rate of perilla, increase the yield and quality of perilla leaves and seeds, and increase the content of α-linolenic acid in perilla. In addition, melatonin can effectively alleviate the growth inhibition effect of pathogen effector protein flagellin 22 on perilla and reduce the persecution effect of pathogens on perilla. Melatonin can not only promote the growth and development of perilla and improve the stress resistance of perilla, but also increase the content of perilla oil and α-linolenic acid in perilla oil. DETAILED DESCRIPTION

[0029] The technical solution of the present invention is further described below in conjunction with the embodiments, but is not limited thereto. Any modification or equivalent replacement of the technical solution of the present invention without departing from the spirit and scope of the technical solution of the present invention should be included in the protection scope of the present invention.

[0030] The test will be conducted on May 5, 2022 and May 4, 2023. The test site is located in the experimental base of Daqing Branch of Heilongjiang Academy of Sciences in Datong District, Daqing City, Heilongjiang Province, in the southwest of Daqing City. It is located in the western part of Songnen Plain, with a jurisdiction area of ​​2,400 square kilometers and a total cultivated land area of ​​92.7km 2. It belongs to the temperate continental monsoon climate, with an annual accumulated temperature of about 2800℃, an average annual rainfall of 400mm, a frost-free period of 148d, an average annual temperature of 3℃, and belongs to the first accumulated temperature zone. The test site is carbonate chernozem. The soil contains 121.73mg / kg of alkaline nitrogen, 20.64mg / kg of available phosphorus, 137.50mg / kg of available potassium, 14.32% of organic matter, and a pH of 7.51. Before arranging the plot experiment, the humic acid bio-organic fertilizer made by the Heilongjiang Academy of Sciences was applied as the base fertilizer, and the humic acid fertilizer involved in the application number 202010589789.4 or the application number 201710655921.5 was used.

[0031] Embodiment 1:

[0032] The experiment selected "Long Zi Su No. 1" with a thousand-grain weight of 4.5, which was independently developed by the Daqing Branch of Heilongjiang Academy of Sciences and suitable for planting in Heilongjiang, as the test material, and selected the perilla seeds with full grains, uniform size and high maturity.

[0033] The Bacillus strain purchased from the market was streaked on TSA solid medium and cultured in an incubator at 28-30°C in the dark. After the colonies with an average diameter of more than 1 mm visible to the naked eye grew, they were inoculated on TSA liquid medium and cultured on a shaker at 35°C and 180 rpm in the dark. After 5 days, when the culture solution became turbid, the shaking culture was stopped to obtain a concentration of 1×10 9 cfu·mL -1 The Bacillus fermentation broth is cooled naturally and set aside.

[0034] Prepare 100 μmol / L melatonin solution, place in a brown bottle, and store in a 4°C refrigerator for later use.

[0035] Preparation process of perilla seed adsorbent formula (500g): 273.5g of roasted tobacco powder, 100g of hemp leaf powder, 100g of flax leaf powder, 20.5g of Ca(H2PO4), 1g of sucrose, 4g of lime powder, 1g of capsanthin, 1mL of 0.5% NaMoO4, 1mL of 0.5% H3BO3, stir well and set aside. Bacillus fermentation broth (1×109 cfu·mL -1 ) is added to the prepared adsorbent in a mass ratio of 1:2 to prepare a Bacillus adsorbent. 5 mL of 4% sodium hydroxymethylcellulose adhesive is added to 15 g of the Bacillus adsorbent, and 100 g of perilla seeds soaked in 100 μmol / L melatonin solution for 4 h are added, and stirred repeatedly to make the perilla seeds adsorbed by the Bacillus adsorbent, and then 30 g of bentonite is added and stirred evenly. At this time, each seed has a layer of red coating material. The coated perilla seeds are placed in a cool, dry and ventilated place and dried in the shade for use.

[0036] Embodiment 2:

[0037] The experiment selected "Long Zi Su No. 1" with a thousand-grain weight of 4.5, which was independently developed by the Daqing Branch of Heilongjiang Academy of Sciences and suitable for planting in Heilongjiang, as the test material, and selected the perilla seeds with full grains, uniform size and high maturity.

[0038] The Bacillus strain purchased from the market was streaked on TSA solid medium and cultured in an incubator at 28-30°C in the dark. After the colonies with an average diameter of more than 1 mm visible to the naked eye grew, they were inoculated on TSA liquid medium and cultured on a shaker at 35°C and 180 rpm in the dark. After 5 days, when the culture solution became turbid, the shaking culture was stopped to obtain a concentration of 1×10 9 cfu·mL -1 The Bacillus fermentation broth is cooled naturally and set aside.

[0039] Prepare 100 μmol / L melatonin solution, place in a brown bottle, and store in a 4°C refrigerator for later use.

[0040] Preparation process of perilla seed adsorbent formula (500g): 273.5g of roasted tobacco powder, 100g of hemp leaf powder, 100g of flax leaf powder, 20.5g of Ca(H2PO4), 1g of sucrose, 5g of lime powder, 1mL of 0.5% NaMoO4, 1mL of 0.5% H3BO3, stir well and set aside. Bacillus fermentation broth (1×109 cfu·mL -1 ) is added to the prepared adsorbent in a mass ratio of 1:2 to prepare a Bacillus adsorbent. 5 mL of 4% sodium hydroxymethylcellulose adhesive is added to 15 g of the Bacillus adsorbent, and 100 g of perilla seeds soaked in 100 μmol / L melatonin solution for 4 h are added, and stirred repeatedly to make the perilla seeds adsorbed by the Bacillus adsorbent, and then 30 g of bentonite is added and stirred evenly. At this time, each seed has a layer of brown coating material. The coated perilla seeds are placed in a cool, dry and ventilated place and dried in the shade for use.

[0041] Embodiment 3:

[0042] The experiment selected "Long Zi Su No. 1" with a thousand-grain weight of 4.5, which was independently developed by the Daqing Branch of Heilongjiang Academy of Sciences and suitable for planting in Heilongjiang, as the test material, and selected the perilla seeds with full grains, uniform size and high maturity.

[0043] The Bacillus strain purchased from the market was streaked on TSA solid medium and cultured in an incubator at 28-30°C in the dark. After the colonies with an average diameter of more than 1 mm visible to the naked eye grew, they were inoculated on TSA liquid medium and cultured on a shaker at 35°C and 180 rpm in the dark. After 5 days, when the culture solution became turbid, the shaking culture was stopped to obtain a concentration of 1×10 9 cfu·mL -1 The Bacillus fermentation broth is cooled naturally and set aside.

[0044] Prepare 100 μmol / L melatonin solution, place in a brown bottle, and store in a 4°C refrigerator for later use.

[0045] Preparation process of perilla seed adsorbent formula (500g): peat soil 473.5g, Ca(H2PO4) 20.5g, sucrose 1g, lime powder 5g, 0.5% NaMoO4 1mL, 0.5% H3BO3 1mL, stir evenly and set aside. Bacillus fermentation broth (1×109 cfu·mL -1 ) is added to the prepared adsorbent in a mass ratio of 1:2 to prepare a Bacillus adsorbent. 5 mL of 4% sodium hydroxymethylcellulose adhesive is added to 15 g of the Bacillus adsorbent, and 100 g of perilla seeds soaked in 100 μmol / L melatonin solution for 4 h are added, and stirred repeatedly to make the perilla seeds adsorbed by the Bacillus adsorbent, and then 30 g of bentonite is added and stirred evenly. At this time, each seed has a layer of brown coating material. The coated perilla seeds are placed in a cool, dry and ventilated place and dried in the shade for use.

[0046] Comparative Example 1:

[0047] The perilla seeds were carefully selected, and those with full grains, uniform size and high maturity were selected. After being immersed in 100 μmol / L melatonin solution for 4 hours, they were naturally air-dried and set aside for use.

[0048] Comparative Example 2:

[0049] Select the perilla seeds carefully, and choose those with full grains, uniform size and high maturity, and set aside.

[0050] The materials in the above examples and comparative examples were sown in holes, with a fixed sowing amount of 8 seeds per hole. The planting results are shown in Tables 1 and 2 below.

[0051] The planting results of the above embodiments and comparative examples are shown in Tables 1 and 2 below.

[0052] Table 1 2022 Perilla test results

[0053]

[0054]

[0055] Table 2 2023 Perilla test results

[0056]

Claims

1. A planting method for improving the germination rate of perilla in high-cold areas, characterized in that: The method is: Step 1: Select perilla seeds: Choose perilla seeds with full grains, uniform size and high maturity; Step 2: The Bacillus strain purchased from the market was streaked on TSA solid medium and placed in an incubator at 28-30°C in the dark. After the colonies with an average diameter of more than 1 mm visible to the naked eye grew, they were inoculated on TSA liquid medium and placed on a shaker at 32-38°C and 170-190 rpm in the dark. After 5 days, when the culture solution became turbid, the shaking culture was stopped to obtain a concentration of 1×10 9 cfu·mL -1 The Bacillus fermentation liquid is cooled naturally and set aside; Step 3: Prepare 90-110 μmol / L melatonin solution, place in a brown bottle, and store in a 4°C refrigerator for later use; Step 4: Preparation process of perilla seed adsorbent formula: 50%-60% roasted tobacco powder, 16%-24% hemp leaf powder, 16%-24% flax leaf powder, 4.1% Ca(H2PO4), 0.2% sucrose, 0.8% lime powder, 0.2% capsanthin; add 1mL 0.5vol.% NaMoO4 and 1mL 0.5vol.% H3BO3 to every 500g of the above raw materials, stir well and set aside; Step 5: Bacillus fermentation liquid and adsorbent are mixed in a mass ratio of 1:2 to prepare Bacillus adsorbent; 5 mL of 4% sodium hydroxymethylcellulose adhesive is added to 15 g of Bacillus adsorbent, and 100 g of perilla seeds soaked in the melatonin solution obtained in step 3 for 4 hours are added, and the mixture is stirred repeatedly so that the perilla seeds are all adsorbed by the Bacillus adsorbent, and 30 g of bentonite is added and stirred evenly. At this time, each seed has a layer of red coating material. The coated perilla seeds are placed in a cool, dry and ventilated place and dried in the shade for use.

2. A planting method for improving the germination rate of perilla in high-cold areas, characterized in that: The method is: Step 1: Select perilla seeds: Choose perilla seeds with full grains, uniform size and high maturity; Step 2: The Bacillus strain purchased from the market was streaked on TSA solid medium and placed in an incubator at 28-30°C in the dark. After the colonies with an average diameter of more than 1 mm visible to the naked eye grew, they were inoculated on TSA liquid medium and placed on a shaker at 32-38°C and 170-190 rpm in the dark. After 5 days, when the culture solution became turbid, the shaking culture was stopped to obtain a concentration of 1×10 9 cfu·mL -1 The Bacillus fermentation liquid is cooled naturally and set aside; Step 3: Prepare 90-110 μmol / L melatonin solution, place in a brown bottle, and store in a 4°C refrigerator for later use; Step 4: Preparation process of perilla seed adsorbent formula: 50%-60% roasted tobacco powder, 16%-24% hemp leaf powder, 16%-24% flax leaf powder, 4.1% Ca(H2PO4), 0.2% sucrose, 1% lime powder; add 1mL 0.5vol.% NaMoO4 and 1mL 0.5vol.% H3BO3 to every 500g of the above raw materials, stir well and set aside; Step 5: Bacillus fermentation liquid and adsorbent are mixed in a mass ratio of 1:2 to prepare Bacillus adsorbent; 5 mL of 4% sodium hydroxymethylcellulose adhesive is added to 15 g of Bacillus adsorbent, and 100 g of perilla seeds soaked in the melatonin solution obtained in step 3 for 4 hours are added, and the mixture is stirred repeatedly so that the perilla seeds are all adsorbed by the Bacillus adsorbent, and 30 g of bentonite is added and stirred evenly. At this time, each seed has a layer of brown coating material. The coated perilla seeds are placed in a cool, dry and ventilated place and dried in the shade for use.

3. A planting method for improving the germination rate of Perilla in high-cold areas, characterized in that: The method is: Step 1: Select perilla seeds: Choose perilla seeds with full grains, uniform size and high maturity; Step 2: The Bacillus strain purchased from the market was streaked on TSA solid medium and placed in an incubator at 28-30°C in the dark. After the colonies with an average diameter of more than 1 mm visible to the naked eye grew, they were inoculated on TSA liquid medium and placed on a shaker at 32-38°C and 170-190 rpm in the dark. After 5 days, when the culture solution became turbid, the shaking culture was stopped to obtain a concentration of 1×10 9 cfu·mL -1 The Bacillus fermentation liquid is cooled naturally and set aside; Step 3: Prepare 90-110 μmol / L melatonin solution, place in a brown bottle, and store in a 4°C refrigerator for later use; Step 4: Preparation process of perilla seed adsorbent formula: peat soil 94%-96%, Ca(H2PO4) 2.8%-4.7%, sucrose 0.2%, lime powder 1%; add 1mL 0.5vol.% NaMoO4 and 1mL 0.5vol.% H3BO3 to every 500g of the above raw materials, stir well and set aside; Step 5: Bacillus fermentation liquid and adsorbent are mixed in a mass ratio of 1:2 to prepare Bacillus adsorbent; 5 mL of 4% sodium hydroxymethylcellulose adhesive is added to 15 g of Bacillus adsorbent, and 100 g of perilla seeds soaked in the melatonin solution obtained in step 3 for 4 hours are added, and the mixture is stirred repeatedly so that the perilla seeds are all adsorbed by the Bacillus adsorbent, and 30 g of bentonite is added and stirred evenly. At this time, each seed has a layer of brown coating material. The coated perilla seeds are placed in a cool, dry and ventilated place and dried in the shade for use.

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