Automatic culture medium split charging equipment

Abstract

The invention provides automatic culture medium split charging equipment. The automatic culture medium split charging equipment comprises a base, the first moving assembly is arranged on the upper wall of the base; the variable configuration type wriggling assembly is arranged on one side of the base; the quantitative supporting assembly is movably arranged on the first moving assembly; the second moving assembly is arranged on one side of the base, and the second moving assembly is arranged on one side of the variable-configuration type wriggling assembly; the third moving assembly is arranged on the second moving assembly; a peristaltic pipe assembly is arranged on the third moving assembly, the sub-packaging process is divided into multiple stages, a peristaltic pump can correspondingly deform in each stage, then flow pulsation in the peristaltic process is effectively reduced, and the accuracy in the sub-packaging process is guaranteed.

Description

Technical Field

[0001] The invention belongs to the technical field of experimental instruments, and in particular relates to an automatic culture medium dispensing device. Background Art

[0002] The culture medium automatic filling equipment is a scientific instrument used in the fields of biology, agronomy, and chemical engineering, which aims to improve the efficiency and accuracy of culture medium preparation and filling.

[0003] However, there are certain problems in the packaging process of the culture medium. A peristaltic pump is often required in the packaging process of the culture medium. Since there will be certain flow pulsation in the existing peristaltic process, the flow pulsation will often cause inaccurate flow in the packaging process. Summary of the invention

[0004] In view of the above situation, in order to overcome the defects of the prior art, the present invention provides an automatic culture medium filling device, which divides the filling process into multiple stages. In each stage, the peristaltic pump will deform accordingly, thereby effectively reducing the flow pulsation during the peristaltic process and ensuring the accuracy of the filling process.

[0005] The technical solution adopted by the present invention is as follows: The present invention provides an automatic culture medium dispensing device, including a base; a first movable component installed on the upper wall of the base; a variable configuration peristaltic component installed on one side of the base; a quantitative support component movably arranged on the first movable component; a second movable component installed on one side of the base, and the second movable component is installed on one side of the variable configuration peristaltic component; a third movable component installed on the second movable component; and a peristaltic tube assembly is installed on the third movable component.

[0006] Furthermore, the variable configuration peristaltic assembly includes a first support plate installed on one side of the base, a second support plate is fixedly provided on the side wall of the first support plate, a first ratchet is rotatably provided on the second support plate, and a first pressure block is installed on the upper end of the second support plate.

[0007] Further,

[0008] A triangular support plate is installed on one side of the first ratchet wheel, a first rotating plate is rotatably arranged on the triangular support plate, the triangular support plate is designed in a triangular shape, and the first rotating plates are distributed at three ends of the triangular support plate;

[0009] A first rotating rod is rotatably connected between the first rotating plate and the first ratchet wheel, the first rotating rod is designed in an arc shape, and the first rotating plate is designed in a crescent shape;

[0010] A first roller is rotatably provided on the first rotating plate, a second ratchet is rotatably provided in cooperation with the first ratchet, and the second ratchet is rotatably provided on the second supporting plate.

[0011] Furthermore, a first connection point is provided between the first rotating rod and the first rotating plate, a second connection point is provided between the first rotating plate and the triangular support plate, a third connection point is provided between the first roller and the first rotating plate, the first connection point rotates around the second connection point at an angle range of 0°-50°, a first motor is installed on one side of the first ratchet, the first motor is installed on one side of the first support plate, a second motor is installed on one side of the second ratchet, the second motor is installed on one side of the first support plate, the first motor drives a first main shaft, the first ratchet is rotatably arranged on the first main shaft, a third ratchet is provided on the inner side of the first ratchet, a fourth ratchet is provided on the first main shaft, the third ratchet is matched with the fourth ratchet in transmission; the triangular support plate is fixed on the first main shaft, and the second ratchet and the fourth ratchet have opposite rotation directions.

[0012] Furthermore, the quantitative support assembly includes a first movable plate slidably disposed on the first movable assembly, and a culture medium fixing plate is mounted on the first movable plate.

[0013] Furthermore, the first moving assembly includes a first slide rail installed on the base, a first moving plate is slidably provided on the first slide rail, and a linear motor drives the first moving plate and the first slide rail.

[0014] Furthermore, the second moving assembly includes a second fixed plate installed on the side wall of the base, the second fixed plate is installed with a second slide rail, the second moving plate is slidably provided on the second slide rail, and the second moving plate and the second slide rail are driven by a linear motor.

[0015] Furthermore, the third movable component includes a third fixed plate installed on a side wall of the second movable plate, the third fixed plate is provided with a third slide rail, the third movable plate is slidably provided on the third slide rail, and the peristaltic tube assembly includes a first drainage tube fixedly provided on the third movable plate, the first drainage tube is connected to a peristaltic hose, the peristaltic hose is placed between the first roller and the first pressing block, one end of the peristaltic hose is also connected to a culture medium holding bottle, and the culture medium holding bottle is fixedly provided on the side wall of the base.

[0016] Furthermore, a culture medium packaging method using an automatic culture medium packaging device comprises the following steps:

[0017] In the first step, the mass of the liquid in the single bottle distributed by the first drainage tube is recorded as M;

[0018] Record the mass of the liquid in a single bottle when the first drainage tube is loaded in real time as m;

[0019] Record the number of bottles loaded as n;

[0020] If 0 < n*m < 0.4*n*M, it is recorded as the initial stage;

[0021] If 0.4*n*M < n*m < 0.8*n*M, it is recorded as the middle stage;

[0022] If 0.8*n*M < n*m < n*M, it is recorded as the end stage of sub-packaging;

[0023] Furthermore, in the initial stage, the first motor starts, drives the triangular support plate to rotate, the liquid flows from the culture medium containing bottle into the first drainage tube, and then into the sub-packaging tube;

[0024] In the middle stage, at this time, the culture medium feeding cycle is divided into a first stage, a second stage, and a third stage. The feeding cycle is the rotation time of the triangular support plate rotating 360°;

[0025] In the first stage, at this time, the second motor drives the second ratchet wheel to rotate, the second ratchet wheel drives the first rotating rod to rotate, the first rotating rod drives the first rotating plate to rotate until the first connection point forms a 20° angle relative to the second connection point. Subsequently, the first motor starts, drives the triangular support plate to rotate, the liquid flows from the culture medium containing bottle into the first drainage tube, and then into the sub-packaging tube;

[0026] In the second stage, at this time, the second motor drives the second ratchet wheel to rotate, the second ratchet wheel drives the first rotating rod to rotate, the first rotating rod drives the first rotating plate to rotate until the first connection point forms a 40° angle relative to the second connection point. Subsequently, the first motor starts, drives the triangular support plate to rotate, the liquid flows from the culture medium containing bottle into the first drainage tube, and then into the sub-packaging tube;

[0027] In the third stage, at this time, the second motor drives the second ratchet wheel to rotate, the second ratchet wheel drives the first rotating rod to rotate, the first rotating rod drives the first rotating plate to rotate until the first connection point forms a 35° angle relative to the second connection point. Subsequently, the first motor starts, drives the triangular support plate to rotate, the liquid flows from the culture medium containing bottle into the first drainage tube, and then into the sub-packaging tube;

[0028] In the end stage:

[0029] At this time, the culture medium feeding cycle is divided into a first stage, a second stage, a third stage, a fourth stage, and a fifth stage. The feeding cycle is the rotation time of the triangular support plate rotating 360°;

[0030] In the first stage, the second motor drives the second ratchet to rotate, the second ratchet drives the first rotating rod to rotate, and the first rotating rod drives the first rotating plate to rotate until the first connection point is at an angle of 20° relative to the second connection point; then the first motor starts, driving the triangular support plate to rotate, and the liquid flows from the culture medium holding bottle into the first drainage tube, and then into the filling tube.

[0031] In the second stage, the second motor drives the second ratchet to rotate, the second ratchet drives the first rotating rod to rotate, and the first rotating rod drives the first rotating plate to rotate until the first connection point is at a 35° angle relative to the second connection point; then the first motor starts, driving the triangular support plate to rotate, and the liquid flows from the culture medium holding bottle into the first drainage tube, and then into the filling tube.

[0032] In the third stage, the second motor drives the second ratchet to rotate, the second ratchet drives the first rotating rod to rotate, and the first rotating rod drives the first rotating plate to rotate until the first connection point is at a 40° angle relative to the second connection point; then the first motor starts, driving the triangular support plate to rotate, and the liquid flows from the culture medium holding bottle into the first drainage tube, and then into the filling tube.

[0033] In the fourth stage, the second motor drives the second ratchet to rotate, the second ratchet drives the first rotating rod to rotate, and the first rotating rod drives the first rotating plate to rotate until the first connection point is at an angle of 50° relative to the second connection point; then the first motor starts, driving the triangular support plate to rotate, and the liquid flows from the culture medium holding bottle into the first drainage tube, and then into the filling tube.

[0034] In the fifth stage, the second motor drives the second ratchet to rotate, the second ratchet drives the first rotating rod to rotate, and the first rotating rod drives the first rotating plate to rotate until the first connection point is at an angle of 35° relative to the second connection point. Then the first motor starts, driving the triangular support plate to rotate, and the liquid flows from the culture medium holding bottle into the first drainage tube, and then into the dispensing tube;

[0035] Beneficial effects of the present invention: Based on the above technical solution, the present invention drives the second ratchet wheel through the second motor, and then the second ratchet wheel drives the first rotating plate to rotate. Through the ingenious design structure, by accurately controlling the rotation angle of the first roller, the compression gap between the first roller and the peristaltic hose can be effectively and accurately controlled, thereby improving the flow pulsation during the peristaltic process;

[0036] In addition, by designing the form of linear motor drive, the mobile positioning of the working process can be promoted quickly, conveniently and accurately, ensuring fast and accurate positioning. At the same time, the entire structure is compactly designed to ensure that it does not take up too much space.

[0037] At the same time, packaging methods for different stages are designed, which can efficiently perform quantitative packaging according to the packaging process and ensure the accuracy of the packaging process. BRIEF DESCRIPTION OF THE DRAWINGS

[0038] Figure 1 A main structure diagram of an automatic culture medium dispensing device provided in this embodiment;

[0039] Figure 2 A front view of an automatic culture medium dispensing device provided in this embodiment;

[0040] Figure 3 The main structure diagram of the variable configuration peristaltic component provided in this embodiment;

[0041] Figure 4 A diagram of the first roller installation structure provided in this embodiment;

[0042] Figure 5 for Figure 4 A local enlarged view of the M part;

[0043] Figure 6 A schematic diagram of the installation structure of the first connection point, the second connection point and the third connection point provided in this embodiment;

[0044] Figure 7 A rear view of an automatic culture medium dispensing device provided in this embodiment;

[0045] Figure 8 A side view of an automatic culture medium dispensing device provided in this embodiment;

[0046] Fig. 9 A diagram showing the connection structure of the third ratchet and the fourth ratchet provided in this embodiment;

[0047] Fig.10 A diagram showing the relationship between the rubber tube gap and the instantaneous flow rate provided in this embodiment;

[0048] Fig.11 A flow rate fluctuation comparison diagram of an automatic culture medium dispensing device provided in this embodiment before and after improvement;

[0049] Description of the accompanying drawings: 1. base, 2. first moving component, 201. first slide rail, 3. variable configuration peristaltic component, 301. first support plate, 302. second support plate, 303. first ratchet, 3031. third ratchet, 304. first pressing block, 305. triangular support plate, 306. first rotating plate, 307. first rotating rod, 308. first roller, 309. second ratchet, 310. first motor, 311. second motor, 3131. fourth ratchet, 3071. first A connection point, 3072, a second connection point, 3073, a third connection point, 4, a quantitative support assembly, 401, a first movable plate, 402, a culture medium fixing plate, 5, a second movable assembly, 501, a second fixed plate, 502, a second slide rail, 503, a second movable plate, 6, a third movable assembly, 601, a third fixed plate, 602, a third slide rail, 603, a third movable plate, 7, a peristaltic tube assembly, 701, a first drainage tube, 702, a peristaltic hose, 703, a culture medium holding bottle.

[0050] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention. DETAILED DESCRIPTION

[0051] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0052] In the description of the present invention, it should be understood that terms such as “upper”, “lower”, “front”, “back”, “left”, “right”, “top”, “bottom”, “inside” and “outside” indicating directions or positional relationships are based on the directions or positional relationships shown in the accompanying drawings, and are only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific direction, be constructed and operated in a specific direction, and therefore should not be understood as limiting the present invention.

[0053] Embodiment 1:

[0054] See also Figure 1The present embodiment provides an automatic culture medium dispensing device, comprising a base 1; a first moving component 2 mounted on the upper wall of the base 1; a variable configuration peristaltic component 3 mounted on one side of the base 1; a quantitative support component 4 movably mounted on the first moving component 2; a second moving component 5 mounted on one side of the base 1, and the second moving component 5 is mounted on one side of the variable configuration peristaltic component 3; a third moving component 6 mounted on the second moving component 5; and a peristaltic tube component 7 is mounted on the third moving component 6.

[0055] See also Figure 1-Figure 5 In some embodiments, the deformable peristaltic component 3 includes a first support plate 301 installed on one side of the base 1, a second support plate 302 is fixedly provided on the side wall of the first support plate 301, a first ratchet 303 is rotatably provided on the second support plate 302, and a first pressure block 304 is installed on the upper end of the second support plate 302.

[0056] See also Figure 1-Figure 5 In some embodiments, a triangular support plate 305 is installed on one side of the first ratchet 303, and a first rotating plate 306 is rotatably provided on the triangular support plate 305, and the triangular support plate 305 is designed in a triangular shape, and the first rotating plates 306 are distributed at the three ends of the triangular support plate 305; a first rotating rod 307 is rotatably connected between the first rotating plate 306 and the first ratchet 303, and the first rotating rod 307 is designed in an arc shape, and the first rotating plate is designed in a crescent shape; a first roller 308 is rotatably provided on the first rotating plate 306, and a second ratchet 309 is rotatably provided in cooperation with the first ratchet 303, and the second ratchet 309 is rotatably provided on the second support plate 302.

[0057] See also Figure 1-Figure 8In some embodiments, a first connection point 3071 is provided between the first rotating rod 307 and the first rotating plate 306, a second connection point 3072 is provided between the first rotating plate 306 and the triangular support plate 305, a third connection point 3073 is provided between the first roller 308 and the first rotating plate 306, the first connection point 3071 rotates around the second connection point in a range of 0°-50°, a first motor 310 is installed on one side of the first ratchet 303, the first motor 310 is installed on one side of the first support plate 301, and the second ratchet 3 09 is provided with a second motor 311 on one side, the second motor 311 is provided on one side of the first support plate 301, the first motor 310 drives a first main shaft 313, the first ratchet 308 is rotatably provided on the first main shaft 313, a third ratchet 3031 is provided on the inner side of the first ratchet 303, a fourth ratchet 3131 is provided on the first main shaft, the third ratchet 3031 is matched with the fourth ratchet 3131 in transmission; the triangular support plate 305 is fixed on the first main shaft 313, the second ratchet 309 and the fourth ratchet 3131 rotate in opposite directions.

[0058] See also Figure 1 In some embodiments, the quantitative support component 4 includes a first movable plate 401 slidably disposed on the first movable component 2 , and a culture medium fixing plate 402 is mounted on the first movable plate 401 .

[0059] See also Figure 1 In some embodiments, the first moving component 2 includes a first slide rail 201 installed on the base 1, a first moving plate 401 is slidably provided on the first slide rail 201, and a linear motor drive is used between the first moving plate 401 and the first slide rail 201.

[0060] See also Figure 1 and Figure 2 In some embodiments, the second moving component 5 includes a second fixed plate 501 installed on the side wall of the base 1, the second fixed plate 501 is equipped with a second slide rail 502, the second moving plate 503 is slidably provided on the second slide rail 502, and the second moving plate 503 and the second slide rail 502 are driven by a linear motor.

[0061] See also Figure 1 , Figure 2 and Figure 8In some embodiments, the third movable component 6 includes a third fixed plate 601 installed on a side wall of the second movable plate 503, a third slide rail 602 is installed on the third fixed plate 601, and a third movable plate 603 is slidably provided on the third slide rail 602, and the peristaltic tube assembly 7 includes a first drainage tube 701 fixed on the third movable plate 603, a peristaltic hose 702 is connected to the first drainage tube 701, and the peristaltic hose 702 is placed between the first roller 308 and the first pressing block 304, and one end of the peristaltic hose 702 is also connected to a culture medium holding bottle 703, and the culture medium holding bottle 703 is fixed on the side wall of the base 1.

[0062] Specific working process:

[0063] The first movable plate 401 drives the culture medium fixing plate 402 to move on the first slide rail 201, and at the same time drives the second ratchet 309 to rotate by driving the second motor 311, thereby driving the first ratchet 303 that cooperates with the second ratchet 309 to rotate, and then the first ratchet 303 drives the first rotating rod 307 to rotate, and the first rotating rod 307 drives the first rotating plate 306 to rotate, and then the first rotating plate 306 drives the first roller 308 to rotate.

[0064] When the second motor 311 is driven, the first roller 308 reaches the specified rotation angle, and the first motor 310 rotates, driving the triangular support plate 305 to rotate in the opposite direction, and then the triangular support plate 305 drives the first rotating plate 306 to rotate. At this time, due to the action of the third ratchet 3031 and the fourth ratchet 3131, the first ratchet 303 is not fixed on the first motor 310, and the first motor 310 only drives the triangular support plate 305 to rotate, and then the first roller 308 squeezes and peristalticizes the peristaltic hose 702, and the peristaltic pressure drives the culture medium from the culture medium bottle 703 to the first drainage tube 701.

[0065] Example 2

[0066] The diameter of the first roller 308 used in this embodiment is 13.8 mm, the inner diameter of the peristaltic hose 702 is 7 mm, and the diameter of the first pressing block 304 is 48.2 mm.

[0067] A culture medium dispensing method, using a culture medium automatic dispensing device, a culture medium fixing plate 402 is provided with a plurality of bottles for placing culture fluid, comprising the following steps:

[0068] In the first step, the mass of the liquid in the single bottle distributed by the first drainage tube 701 is recorded as M;

[0069] The mass of liquid loaded into a single bottle by the first drainage tube 701 in real time is recorded as m;

[0070] Record the number of bottles filled as n;

[0071] If 0 < n*m < 0.4*n*M, it is recorded as the initial stage;

[0072] If 0.4*n*M < n*m < 0.8*n*M, it is recorded as the middle stage;

[0073] If 0.8*n*M < n*m < n*M, it is recorded as the end stage of sub-packaging;

[0074] In the initial stage,

[0075] The first motor 310 starts, driving the triangular support plate 305 to rotate. The liquid flows from the culture solution containing bottle 703 into the first drainage tube 701, and then into the bottles;

[0076] In the middle stage, since there is still a certain range from reaching the required culture solution quality, the influence of flow pulsation on the sub-packaging process is not too great at this time, so it is divided into 3 stages.

[0077] At this time, the culture solution feeding cycle is divided into the first stage, the second stage, and the third stage. The feeding cycle is the rotation time for the triangular support plate 305 to rotate 360°;

[0078] In the first stage, at this time, the second motor 311 drives the second ratchet wheel 309 to rotate. The second ratchet wheel 309 drives the first rotating rod 307 to rotate. The first rotating rod 307 drives the first rotating plate 306 to rotate until the first connection point 3071 forms a 20° angle relative to the second connection point 3072. Subsequently, the first motor 310 starts, driving the triangular support plate 305 to rotate. The liquid flows from the culture solution containing bottle 703 into the first drainage tube 701, and then into the bottles; at this time, due to the small angle and the large gap of the peristaltic rubber tube 702, it can prevent the peristaltic rubber tube 702 from rebounding too much and causing a large backflow;

[0079] In the second stage, at this time, the second motor 311 drives the second ratchet wheel 309 to rotate. The second ratchet wheel 309 drives the first rotating rod 307 to rotate. The first rotating rod 307 drives the first rotating plate 306 to rotate until the first connection point 3071 forms a 40° angle relative to the second connection point 3072. Subsequently, the first motor 310 starts, driving the triangular support plate 305 to rotate. The liquid flows from the culture solution containing bottle 703 into the first drainage tube 701, and then into the bottles;

[0080] In the third stage, the second motor 311 drives the second ratchet 309 to rotate, the second ratchet 309 drives the first rotating rod 307 to rotate, and the first rotating rod 307 drives the first rotating plate 306 to rotate until the first connection point 3071 is at an angle of 35° relative to the second connection point 3072. Then the first motor 310 is started, driving the triangular support plate 305 to rotate, and the liquid flows from the culture liquid holding bottle 703 into the first drainage tube 701, and then into the bottle;

[0081] In the final stage:

[0082] At this time, the culture solution feeding cycle is divided into the first stage, the second stage, the third stage, the fourth stage and the fifth stage, and the feeding cycle is the time for the triangular support plate 305 to rotate 360°;

[0083] In the first stage, the second motor 311 drives the second ratchet 309 to rotate, the second ratchet 309 drives the first rotating rod 307 to rotate, and the first rotating rod 307 drives the first rotating plate 306 to rotate until the first connection point 3071 is at a 20° angle relative to the second connection point 3072; then the first motor starts, driving the triangular support plate to rotate, and the liquid flows from the culture medium holding bottle 703 into the first drainage tube 701, and then flows into the filling tube. At this time, due to the small angle and the large gap of the peristaltic hose 702, the excessive rebound of the peristaltic hose 702 can be prevented to cause a large backflow.

[0084] In the second stage, the second motor 311 drives the second ratchet 309 to rotate, the second ratchet 309 drives the first rotating rod 307 to rotate, and the first rotating rod 307 drives the first rotating plate 306 to rotate until the first connection point 3071 is at a 35° angle relative to the second connection point 3072; then the first motor 310 starts, driving the triangular support plate 305 to rotate, and the liquid flows from the culture medium bottle 703 into the first drainage tube 701, and then flows into the bottle; at this time, compared with the first stage, the angle increases, and the gap of the peristaltic hose 702 becomes smaller than that of the first stage, which can prevent a large discharge volume and maintain stability in the peristaltic hose 702.

[0085] In the third stage, the second motor 311 drives the second ratchet 309 to rotate, the second ratchet 309 drives the first rotating rod 307 to rotate, and the first rotating rod 307 drives the first rotating plate 306 to rotate until the first connection point 3071 is at a 40° angle relative to the second connection point 3072; then the first motor 310 is started, driving the triangular support plate 305 to rotate, and the liquid flows from the culture medium bottle 703 into the first drainage tube 701, and then flows into the bottle; at this time, compared with the second stage, the angle is further increased, and the gap of the peristaltic hose 702 is further reduced compared with the first stage. At this time, the flow rate is large, and a smaller gap of the peristaltic hose 702 is required to maintain stability.

[0086] In the fourth stage, the second motor 311 drives the second ratchet 309 to rotate, the second ratchet 309 drives the first rotating rod 307 to rotate, and the first rotating rod 307 drives the first rotating plate 306 to rotate until the first connection point 3071 is at a 50° angle relative to the second connection point 3072; then the first motor 310 starts, driving the triangular support plate 305 to rotate, and the liquid flows from the culture medium bottle 703 into the first drainage tube 701, and then flows into the bottle; compared with the third stage, the angle is further increased at this time, the gap of the peristaltic hose 702 reaches the minimum, and the flow rate is reduced.

[0087] In the fifth stage, the second motor 311 drives the second ratchet 309 to rotate, the second ratchet 309 drives the first rotating rod 307 to rotate, and the first rotating rod 307 drives the first rotating plate 306 to rotate until the first connection point 3071 is at an angle of 35° relative to the second connection point 3072. Then the first motor 310 is started, driving the triangular support plate 305 to rotate, and the liquid flows from the culture liquid holding bottle 703 into the first drainage tube 701, and then into the bottle; the angle in this stage is the same as that in the second stage, and the gap of the peristaltic hose 702 is the same as that in the second stage, because the flow rate decreases in this stage, and the gap of the peristaltic hose 702 should not be too small.

[0088] like Fig.11 As shown, the peristaltic artery mechanism of dividing a feeding cycle of the culture medium into the first stage, the second stage, the third stage, the fourth stage and the fifth stage, wherein the feeding cycle is the time for the triangular support plate 305 to rotate 360°, and according to Fig.10 It can be found that the larger the creeping gap, the greater the instantaneous flow rate, and thus the greater the flow velocity;

[0089] In the first stage, the peristaltic hose 702 squeezed by the first roller 308 begins to rebound and expand, and negative pressure is generated inside the peristaltic hose 702, causing liquid to reflux in the first drainage tube 701. The reflux amount continues to increase, so the flow rate at the outlet continues to decrease.

[0090] In the second stage, although there will still be liquid reflux, the reflux volume will gradually decrease and the discharge volume will gradually increase, so the flow curve is gradually rising.

[0091] In the third stage, the first roller 308 mainly stretches the peristaltic hose 702, the volume of the peristaltic hose 702 changes slightly, the outlet flow rate of the first drainage tube 701 changes slightly, and therefore the flow curve changes slightly.

[0092] In the fourth stage, the first roller 308 continues to rotate, the peristaltic hose 702 is compressed, the outlet flow rate increases, and the flow curve fluctuates and rises in a small range again.

[0093] In the fifth stage, as the first roller 308 continues to rotate, the liquid sealing area is gradually formed. During this process, the flow velocity in the flow area on both sides of the peristaltic hose 702 will decrease, so the flow curve continues to decrease.

[0094] See also Fig.11 By designing an automatic culture medium filling equipment combined with a culture medium filling method, the improvements before, after 3 stages of improvement and after 5 stages of improvement are compared; it can be seen from the figure that after the improvement, the flow pulsation in the peristaltic process can be better improved, ensuring that the flow pulsation amplitude in the filling process is not too large, thereby improving the flow accuracy of the filling process of the filling device, especially dividing the filling process into the initial stage, middle stage and final stage, especially improving the middle stage and final stage respectively, including improving the 3 stages of the peristaltic process and changing it to 5 stages of the peristaltic process, which can ensure the filling speed while also quickly ensuring the filling accuracy.

[0095] It should be noted that, in this article, relational terms such as first and second, etc. are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any such actual relationship or order between these entities or operations. Moreover, the terms "include", "comprise" or any other variants thereof are intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements includes not only those elements, but also other elements not explicitly listed, or also includes elements inherent to such process, method, article or device.

[0096] Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions and variations may be made to the embodiments without departing from the principles and spirit of the present invention, and that the scope of the present invention is defined by the appended claims and their equivalents.

[0097] The present invention and its embodiments are described above, and such description is not restrictive. The drawings show only one embodiment of the present invention, and the actual structure is not limited thereto. In short, if ordinary technicians in the field are inspired by it, without departing from the purpose of the invention, they can design a structure and embodiment similar to the technical solution without creativity, which should belong to the protection scope of the present invention.

Claims

1. An automatic culture medium dispensing device, characterized in that: include: Base (1); A first moving assembly (2) is mounted on the upper wall of the base (1); A variable configuration peristaltic component (3) is mounted on one side of the base (1); A quantitative support component (4) movably disposed on the first movable component (2); A second moving component (5) is mounted on one side of the base (1), and the second moving component (5) is mounted on one side of the variable configuration peristaltic component (3); A third moving component (6) is mounted on the second moving component (5); The third moving assembly (6) is provided with a peristaltic tube assembly (7).

2. The automatic culture medium dispensing device according to claim 1, characterized in that: The variable configuration peristaltic assembly (3) comprises a first support plate (301) mounted on one side of the base (1); a second support plate (302) is fixedly mounted on the side wall of the first support plate (301); a first ratchet (303) is rotatably mounted on the second support plate (302); and a first pressure block (304) is mounted on the upper end of the second support plate (302).

3. The automatic culture medium dispensing device according to claim 2, characterized in that: A triangular support plate (305) is installed on one side of the first ratchet wheel (303), a first rotating plate (306) is rotatably provided on the triangular support plate (305), the triangular support plate (305) is designed in a triangular shape, and the first rotating plates (306) are distributed at three ends of the triangular support plate (305); A first rotating rod (307) is rotatably connected between the first rotating plate (306) and the first ratchet wheel (303), the first rotating rod (307) is designed in an arc shape, and the first rotating plate is designed in a crescent shape; A first roller (308) is rotatably provided on the first rotating plate (306), a second ratchet (309) is rotatably provided in cooperation with the first ratchet (303), and the second ratchet (309) is rotatably provided on the second supporting plate (302).

4. The automatic culture medium dispensing device according to claim 3, characterized in that: A first connection point (3071) is provided between the first rotating rod (307) and the first rotating plate (306), a second connection point (3072) is provided between the first rotating plate (306) and the triangular support plate (305), a third connection point (3073) is provided between the first roller (308) and the first rotating plate (306), the first connection point (3071) rotates around the second connection point (3072) in a range of 0°-50°, a first motor (310) is provided on one side of the first ratchet (303), the first motor (310) is provided on one side of the first support plate (301), and a third connection point (3073) is provided between the first roller (308) and the first rotating plate (306). A second motor (311) is provided, the second motor (311) is installed on one side of the first support plate (301), the first motor (310) drives a first main shaft (313), the first ratchet (308) is rotatably provided on the first main shaft (313), a third ratchet (3031) is provided inside the first ratchet (303), a fourth ratchet (3131) is provided on the first main shaft (313), the third ratchet (3031) and the fourth ratchet (3131) are transmission-coordinated; the triangular support plate (305) is fixedly provided on the first main shaft (313), and the second ratchet (309) and the fourth ratchet (3131) have opposite rotation directions.

5. The automatic culture medium dispensing device according to claim 4, characterized in that: The quantitative support component (4) comprises a first movable plate (401) slidably arranged on the first movable component (2), and a culture medium fixing plate (402) is mounted on the first movable plate (401).

6. The automatic culture medium dispensing device according to claim 5, characterized in that: The first moving assembly (2) comprises a first slide rail (201) mounted on the base (1), a first moving plate (401) being slidably mounted on the first slide rail (201), and a linear motor driving mode is used between the first moving plate (401) and the first slide rail (201).

7. The automatic culture medium dispensing device according to claim 6, characterized in that: The second moving assembly (5) comprises a second fixed plate (501) mounted on the side wall of the base (1); a second slide rail (502) is mounted on the second fixed plate (501); a second moving plate (503) is slidably mounted on the second slide rail (502); and a linear motor drive is used between the second moving plate (503) and the second slide rail (502).

8. The automatic culture medium dispensing device according to claim 7, characterized in that: The third moving component (6) includes a third fixed plate (601) installed on one side wall of the second moving plate (503). A third sliding rail (602) is installed on the third fixed plate (601). A third moving plate (603) is slidably arranged on the third sliding rail (602). The peristaltic tube assembly (7) includes a first drainage tube (701) fixedly arranged on the third moving plate (603). A peristaltic rubber tube (702) is communicated with the first drainage tube (701). The peristaltic rubber tube (702) is placed between the first roller (308) and the first pressing block (304). One end of the peristaltic rubber tube (702) is also connected with a culture medium holding bottle (703). The culture medium holding bottle (703) is fixedly arranged on the side wall of the base (1).

9. A method for packaging a culture medium, characterized in that: When using a culture medium automatic dispensing device according to any one of claims 1-8, a plurality of bottles are provided on the culture medium fixed plate (402) for placing the culture medium, including the following steps: In the first step, record the total mass of the liquid in a single bottle dispensed by the first drainage tube (701) as M; Record the mass of the liquid in a single bottle that is loaded into the first drainage tube (701) in real time as m; Record the number of bottles loaded as n; If 0 < n*m < 0.4*n*M, it is recorded as the initial stage; If 0.4*n*M < n*m < 0.8*n*M, it is recorded as the middle stage; If 0.8*n*M < n*m < n*M, it is recorded as the end stage of dispensing.

10. According to the culture medium dispensing method described in claim 9, characterized in that In the initial stage, The first motor (310) is started to drive the triangular support plate (305) to rotate. The liquid flows from the culture medium holding bottle (703) into the first drainage tube (701), and then into the bottle; In the middle stage, At this time, the culture medium feeding cycle is divided into a first stage, a second stage, and a third stage. The feeding cycle is the rotation time of the triangular support plate (305) rotating 360°; In the first stage, at this time, the second motor (311) is driven to drive the second ratchet wheel (309) to rotate. The second ratchet wheel (309) drives the first rotating rod (307) to rotate. The first rotating rod (307) drives the first rotating plate (306) to rotate until the first connection point (3071) forms a 20° angle relative to the second connection point (3072). Subsequently, the first motor (310) is started to drive the triangular support plate (305) to rotate. The liquid flows from the culture medium holding bottle (703) into the first drainage tube (701), and then into the bottle; In the second stage, at this time, the second motor (311) is driven to drive the second ratchet wheel (309) to rotate. The second ratchet wheel (309) drives the first rotating rod (307) to rotate. The first rotating rod (307) drives the first rotating plate (306) to rotate until the first connection point (3071) forms a 40° angle relative to the second connection point (3072). Subsequently, the first motor (310) is started to drive the triangular support plate (305) to rotate. The liquid flows from the culture medium holding bottle (703) into the first drainage tube (701), and then into the bottle; In the third stage, the second motor (311) drives the second ratchet (309) to rotate, the second ratchet (309) drives the first rotating rod (307) to rotate, and the first rotating rod (307) drives the first rotating plate (306) to rotate until the first connection point (3071) is at an angle of 35° relative to the second connection point (3072), and then the first motor (310) is started to drive the triangular support plate (305) to rotate, and the liquid flows from the culture liquid holding bottle (703) into the first drainage tube (701), and then flows into the bottle; In the final stage: At this time, a culture solution feeding cycle is divided into a first stage, a second stage, a third stage, a fourth stage and a fifth stage, wherein the feeding cycle is the time for the triangular support plate (305) to rotate 360°; In the first stage, the second motor (311) drives the second ratchet (309) to rotate, the second ratchet (309) drives the first rotating rod (307) to rotate, and the first rotating rod (307) drives the first rotating plate (306) to rotate until the first connection point (3071) is at an angle of 20° relative to the second connection point (3072); then the first motor (310) is started, driving the triangular support plate (305) to rotate, and the liquid flows from the culture liquid holding bottle (703) into the first drainage tube (701), and then into the bottle; In the second stage, the second motor (311) drives the second ratchet (309) to rotate, the second ratchet (309) drives the first rotating rod (307) to rotate, and the first rotating rod (307) drives the first rotating plate (306) to rotate until the first connection point (3071) is at an angle of 35° relative to the second connection point (3072); then the first motor (310) is started, driving the triangular support plate (305) to rotate, and the liquid flows from the culture liquid holding bottle (703) into the first drainage tube (701), and then into the bottle; In the third stage, the second motor (311) drives the second ratchet (309) to rotate, the second ratchet (309) drives the first rotating rod (307) to rotate, and the first rotating rod (307) drives the first rotating plate (306) to rotate until the first connection point (3071) is at an angle of 40° relative to the second connection point (3072); then the first motor (310) is started, driving the triangular support plate (305) to rotate, and the liquid flows from the culture liquid holding bottle (703) into the first drainage tube (701), and then into the bottle; In the fourth stage, the second motor (311) drives the second ratchet (309) to rotate, the second ratchet (309) drives the first rotating rod (307) to rotate, and the first rotating rod (307) drives the first rotating plate (306) to rotate until the first connection point (3071) is at an angle of 50° relative to the second connection point (3072); then the first motor (310) is started, driving the triangular support plate (305) to rotate, and the liquid flows from the culture liquid holding bottle (703) into the first drainage tube (701), and then into the bottle; In the fifth stage, the second motor (311) drives the second ratchet (309) to rotate, the second ratchet (309) drives the first rotating rod (307) to rotate, and the first rotating rod (307) drives the first rotating plate (306) to rotate until the first connection point (3071) is at an angle of 35° relative to the second connection point (3072), and then the first motor (310) is started, driving the triangular support plate (305) to rotate, and the liquid flows from the culture medium bottle (703) into the first drainage tube (701), and then flows into the bottle.

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