Cell water bath shake culture device

Abstract

The invention provides a cell water bath shake culture device, and belongs to the technical field of biological culture. Comprising a heating box, a heating pool is formed in the upper surface of the heating box, a shaking mechanism is arranged in the heating pool, the shaking mechanism comprises an annular plate and a driving part arranged at the center of the bottom face of the heating pool, and the annular plate sleeves the top end of the driving part. By arranging the locking mechanism, when the locking mechanism enables the annular plate to be connected with the driving piece, the driving piece can drive the annular plate, the clamping sleeve, the fixing base and the test tube to rotate, and therefore oscillation of the test tube in the circumferential direction is achieved; when the locking mechanism enables the annular plate to be connected with the heating pool, the driving piece can drive the clamping sleeve to move along the sliding groove, so that the test tube is oscillated in the horizontal direction; in addition, by arranging the vertical moving mechanism, the test tubes can be oscillated in the vertical direction; therefore, the oscillation modes are diversified, different oscillation modes can be selected according to actual cell culture requirements, and culture of different cells is met.

Description

Technical Field

[0001] The invention relates to the technical field of biological culture, and more specifically to a cell water bath shaking culture device. Background Art

[0002] Oscillating culture, also known as suspension culture, refers to a culture method in which microbial cells are inoculated into a liquid culture medium and placed on a shaker or oscillator for continuous shaking. It is widely used in strain screening and microbial expansion culture, and is a commonly used culture method in microbial physiology, biochemistry, fermentation and other life science research fields.

[0003] The main purpose of shaking culture is to simulate the environment in the body, provide a more constant and stable culture condition, promote cell growth and division, and facilitate large-scale cell culture. Through shaking culture, we can better understand the growth law and metabolic process of cells, which provides convenience and methods for cell research and application. However, the existing water bath shakers can only oscillate horizontally or oscillate and mix reagents vertically, resulting in a single oscillation mode that cannot meet various usage requirements; for this reason, a cell water bath shaking culture device is proposed. Summary of the invention

[0004] In view of the problem that the existing water bath shakers can only oscillate horizontally or vertically to mix reagents, resulting in a single oscillation mode and unable to meet various usage requirements, the purpose of the present invention is to provide a cell water bath shaking culture device.

[0005] To solve the above problems, the present invention adopts the following technical solutions:

[0006] A cell water bath shaking culture device, comprising a heating box, a heating pool is provided on the upper surface of the heating box, a shaking mechanism is provided inside the heating pool, the shaking mechanism comprises an annular plate and a driving member provided at the center of the bottom surface of the heating pool, the annular plate is sleeved on the top of the driving member, a plurality of groups of slide grooves are provided on the surface of the annular plate, the slide grooves are provided with jackets inside, the jackets are used to clamp test tubes, a locking mechanism is provided on the upper surface of the annular plate, and the locking mechanism can connect the annular plate to the heating pool or the driving member;

[0007] When the annular plate is connected to the heating pool, the driving member can make the jacket move back and forth inside the slide groove; when the annular plate is connected to the driving member, the driving member can drive the jacket to perform circular motion.

[0008] Optionally, the driving member includes a motor, the output shaft of the motor is fixedly connected to a rotating shaft, the upper end of the rotating shaft is arranged as an I-shaped structure, the annular plate is rotatably sleeved on the outer surface of the recessed portion of the I-shaped structure of the rotating shaft, a pull rope is arranged between the sleeve and the rotating shaft, the pull rope passes through the inner wall of the annular plate, a movable spring is arranged inside the slide groove, one end of the movable spring is fixedly connected to the sleeve, and the other end of the movable spring is fixedly connected to the inner surface of the slide groove.

[0009] Optionally, the locking mechanism includes a sliding seat fixedly mounted on the upper surface of the annular plate, a sliding block is cooperatively mounted inside the sliding seat, limiting rods are fixedly connected on both sides of the outer surface of the sliding block, positioning blocks are slidably connected on both sides of the upper surface of the sliding block, a connecting spring is fixedly connected between the two groups of the positioning blocks, the moving directions of the positioning blocks and the sliding block are perpendicular to each other, a plurality of groups of slots are provided on the outer surface of the sliding seat, the slots include positions a, b, and c, when the positioning block is located at position a, the limiting rod is inserted into the fixed hole on the surface of the rotating shaft; when the positioning block is located at positions b and c, the limiting rod is inserted into the fixed hole on the inner wall of the heating pool.

[0010] Optionally, it also includes a limiting mechanism, which includes a sleeve plate, which is rotatably sleeved on the outer surface of the annular plate, and the upper surface of the annular plate is fixedly connected with a first limiting plate and a second limiting plate at positions on both sides of the slide groove, the outer surface of the annular plate is partially recessed inward to form an arc groove, and the sleeve plate is partially located inside the arc groove and forms a rotating pair therein, an arc spring is arranged inside the arc groove, one end of the arc spring is fixedly connected to the inner wall of the arc groove, and the other end of the arc spring is fixedly connected to the sleeve plate, a connecting rope is fixedly connected between the sleeve plate and the slider, the first limiting plate and the second limiting plate are both configured to be L-shaped, and limiting grooves are opened on the upper surface of the jacket, and the first limiting plate and the second limiting plate can be moved into or out of the limiting groove.

[0011] Optionally, it also includes a vertical moving mechanism, which includes a cylindrical groove opened on one side of the lower surface of the jacket, a guide rod is installed inside the cylindrical groove, the lower end of the guide rod is fixedly connected to a fixing seat, a rubber pad is fixedly installed on the inner surface of the fixing seat, a telescopic spring is sleeved on the outer surface of the guide rod, the upper end of the telescopic spring is fixedly connected to the jacket, the lower end of the telescopic spring is fixedly connected to the fixing seat, a through groove is opened inside the jacket, one end of the pull rope passes through the through groove and is fixedly connected to the fixing seat, a clamping mechanism is provided inside the through groove, and the clamping mechanism is used to fix the pull rope.

[0012] Optionally, the clamping mechanism includes a clamping plate, which is configured as a frame-type structure, the clamping plate, the bottom of the clamping plate is located inside the through groove, the pull rope passes through the inside of the clamping plate, the bottom of the through groove is fixedly connected to a support spring, the upper end of the support spring is fixedly connected to the bottom of the clamping plate, the support spring causes the bottom of the clamping plate to press the pull rope, both sides of the clamping plate pass through the jacket and slide with it, and the top of the clamping plate is configured as a wedge-shaped structure and is arranged inside the limiting groove.

[0013] Optionally, when the positioning block is located at position a, the first limiting plate is located inside the limiting groove; when the positioning block is located at position b, neither the first limiting plate nor the second limiting plate is located inside the limiting groove; when the positioning block is located at position c, the second limiting plate is located inside the limiting groove and presses down the clamp.

[0014] Optionally, the slide grooves are distributed in an annular array with the rotation axis as a base point, and the slide grooves are all arranged along the radial direction of the annular plate.

[0015] Optionally, an inner cavity is opened at the bottom of the heating box, and the motor is fixedly installed inside the inner cavity.

[0016] Optionally, a water injection hole is opened on the upper surface of the heating box, and the lower end of the water injection hole is connected to the heating pool. A drain pipe is fixedly installed at the bottom of the heating pool, and the water outlet end of the drain pipe is located outside the heating box. A valve body is provided inside the drain pipe and the water injection hole.

[0017] Compared with the prior art, the above technical solution provided by the present invention has at least the following beneficial effects:

[0018] In the above scheme, by setting a locking mechanism, when the locking mechanism connects the annular plate with the driving member, the driving member can drive the annular plate, the jacket, the fixing seat and the test tube to rotate, thereby realizing the oscillation of the test tube in the circumferential direction; when the locking mechanism connects the annular plate with the heating pool, the driving member can drive the jacket to move along the slide groove, thereby realizing the horizontal oscillation of the test tube; in addition, by setting a vertical moving mechanism, the vertical oscillation of the test tube can be realized; therefore, the present invention has various oscillation modes, and different oscillation modes can be selected according to actual cell culture needs to meet the culture of different cells.

[0019] By setting a limiting mechanism, when the positioning block is located at position a, it is in the circular motion working state, and at this time the first limiting plate is located inside the limiting groove, the arc spring is in a stretched state, and the first limiting plate can limit the position of the sleeve to prevent the sleeve from moving along the slide groove during circular motion, thereby improving the stability of the sleeve during circular motion. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The accompanying drawings, which are incorporated herein and constitute a part of the specification, illustrate embodiments of the invention and, together with the description, further serve to explain the principles of the invention and to enable those skilled in the relevant art to make and use the invention.

[0021] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0022] Figure 2 is a cross-sectional view of the present invention;

[0023] Figure 3 It is a structural schematic diagram of the shaking mechanism of the present invention;

[0024] Figure 4 It is a structural schematic diagram of the vertical moving mechanism of the present invention;

[0025] Figure 5 It is a structural schematic diagram of the driving member of the present invention;

[0026] Figure 6 It is a structural schematic diagram of the locking mechanism of the present invention;

[0027] Figure 7 For the present invention Figure 6 The enlarged view of point A in the middle;

[0028] Figure 8 It is a structural schematic diagram of the limiting mechanism of the present invention;

[0029] Fig. 9 This is a schematic diagram of the structure when the positioning block of the present invention is located at site a;

[0030] Fig.10 This is a schematic diagram of the structure when the positioning block of the present invention is located at site b;

[0031] Fig.11 This is a schematic diagram of the structure when the positioning block of the present invention is located at the c site.

[0032] [Reference Signs]

[0033] 1. Heating box; 2. Heating pool; 3. Shaking mechanism; 31. Rotating shaft; 32. Annular plate; 33. Jacket; 34. Slide groove; 35. Motor; 36. Pull rope; 37. Moving spring; 4. Locking mechanism; 41. Sliding seat; 42. Sliding block; 43. Limit rod; 44. Positioning block; 45. Connecting spring; 46. Slot; 47. Fixing hole; 5. Limiting mechanism; 51. Sleeve plate; 52. Arc spring; 53. First limiting plate; 54. Limiting groove; 55. Second limiting plate; 56. Connecting rope; 6. Drain pipe; 7. Vertical moving mechanism; 71. Fixed seat; 72. Rubber pad; 73. Telescopic spring; 74. Guide rod; 75. Columnar groove; 76. Through groove; 77. Clamp; 78. Support spring; 8. Inner cavity; 9. Water injection hole.

[0034] As shown in the figure, in order to clearly implement the structure of the embodiment of the present invention, specific structures and devices are marked in the figure, but this is only for illustrative purposes and is not intended to limit the present invention to the specific structure, device and environment. According to specific needs, ordinary technicians in this field can adjust or modify these devices and environments. DETAILED DESCRIPTION

[0035] The following is a detailed description of a cell water bath shaking culture device provided by the present invention in conjunction with the accompanying drawings and specific embodiments. At the same time, it is explained here that in order to make the embodiments more detailed, the following embodiments are the best and preferred embodiments, and those skilled in the art may also adopt other alternatives to implement some known technologies; and the accompanying drawings are only for a more specific description of the embodiments, and are not intended to specifically limit the present invention.

[0036] It should be noted that the references to "one embodiment", "embodiment", "exemplary embodiments", "some embodiments" and the like in the specification indicate that the embodiments described may include specific features, structures or characteristics, but not every embodiment may include the specific features, structures or characteristics. In addition, when a specific feature, structure or characteristic is described in conjunction with an embodiment, it should be within the knowledge of a person skilled in the art to implement such feature, structure or characteristic in conjunction with other embodiments (whether or not explicitly described).

[0037] In general, a term can be understood, at least in part, from its use in context. For example, depending, at least in part, on the context, the term "one or more" as used herein can be used to describe any feature, structure, or characteristic in the singular sense, or can be used to describe a combination of features, structures, or characteristics in the plural sense. Additionally, the term "based on" can be understood as not necessarily intended to convey an exclusive set of factors, but can instead, depending, at least in part, on the context, allow for the presence of other factors that are not necessarily explicitly described.

[0038] It will be understood that the meanings of “on,” “over,” and “above” in the present invention should be interpreted in the broadest manner, so that “on” not only means “directly on” something, but also includes the meaning of being “on” something with intervening features or layers therebetween, and “on” or “over” not only means “on” or “above” something, but also includes the meaning of being “on” or “above” something with no intervening features or layers therebetween.

[0039] Additionally, spatially relative terms such as "under," "beneath," "lower," "above," "upper," and the like may be used herein for descriptive convenience to describe the relationship of one element or feature to another element or features, as shown in the accompanying drawings. Spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the accompanying drawings. The device may be oriented in other ways, and the spatially relative descriptors used herein may be similarly interpreted accordingly.

[0040] like Figures 1 to 4 As shown, an embodiment of the present invention provides a cell water bath shaking culture device, including a heating box 1, a heating pool 2 is provided on the upper surface of the heating box 1, a shaking mechanism 3 is arranged inside the heating pool 2, the shaking mechanism 3 includes an annular plate 32 and a driving member arranged at the center of the bottom surface of the heating pool 2, the annular plate 32 is sleeved on the top of the driving member, a plurality of groups of slide grooves 34 are provided on the surface of the annular plate 32, a jacket 33 is provided inside the slide grooves 34, the jacket 33 is used to clamp the test tube, a locking mechanism 4 is provided on the upper surface of the annular plate 32, the locking mechanism 4 can connect the annular plate 32 to the heating pool 2 or the driving member; the slide grooves 34 are distributed in an annular array with the rotating shaft 31 as the base point, and the slide grooves 34 are all arranged along the radial direction of the annular plate 32.

[0041] When the annular plate 32 is connected to the heating pool 2, the driving member can make the jacket 33 move back and forth inside the slide groove 34; when the annular plate 32 is connected to the driving member, the driving member can drive the jacket 33 to perform circular motion.

[0042] It also includes a vertical moving mechanism 7, which includes a cylindrical groove 75 opened on one side of the lower surface of the jacket 33, a guide rod 74 is installed inside the cylindrical groove 75, the lower end of the guide rod 74 is fixedly connected to a fixing seat 71, a rubber pad 72 is fixedly installed on the inner surface of the fixing seat 71, and a telescopic spring 73 is sleeved on the outer surface of the guide rod 74, the upper end of the telescopic spring 73 is fixedly connected to the jacket 33, and the lower end of the telescopic spring 73 is fixedly connected to the fixing seat 71, a through groove 76 is opened inside the jacket 33, one end of the pull rope 36 passes through the through groove 76 and is fixedly connected to the fixing seat 71, and a clamping mechanism is provided inside the through groove 76, and the clamping mechanism is used to fix the pull rope 36.

[0043] In this embodiment, the fixing seat 71 and the jacket 33 are used to clamp the test tube containing the cell solution, wherein a rubber pad 72 is provided inside the fixing seat 71. When the bottom of the test tube is inserted into the fixing seat 71, there is an interference fit between the test tube and the fixing seat 71. The inner diameter of the jacket 33 should actually be slightly larger than the size of the test tube so that the test tube can move up and down along the jacket 33.

[0044] By setting the locking mechanism 4, when the locking mechanism 4 connects the annular plate 32 with the driving member, the driving member can drive the annular plate 32, the jacket 33, the fixing seat 71 and the test tube to rotate, thereby realizing the oscillation of the test tube in the circumferential direction; when the locking mechanism 4 connects the annular plate 32 with the heating pool 2, the driving member can drive the jacket 33 to move along the slide groove 34, thereby realizing the horizontal oscillation of the test tube; in addition, by setting the vertical moving mechanism 7, the vertical oscillation of the test tube can be realized; therefore, the present invention has various oscillation modes, and different oscillation modes can be selected according to actual cell culture needs to meet the culture of different cells.

[0045] like Figure 2 , Figure 5 as well as Figure 8 As shown, the driving member includes a motor 35, the output shaft of the motor 35 is fixedly connected to the rotating shaft 31, the upper end of the rotating shaft 31 is set as an I-shaped structure, the annular plate 32 is rotatably sleeved on the outer surface of the recessed portion of the I-shaped structure of the rotating shaft 31, a pull rope 36 is arranged between the jacket 33 and the rotating shaft 31, the pull rope 36 passes through the inner wall of the annular plate 32, and a movable spring 37 is arranged inside the slide groove 34, one end of the movable spring 37 is fixedly connected to the jacket 33, and the other end of the movable spring 37 is fixedly connected to the inner surface of the slide groove 34, an inner cavity 8 is opened at the bottom of the heating box 1, and the motor 35 is fixedly installed inside the inner cavity 8.

[0046] like Figures 4 to 7 As shown, the locking mechanism 4 includes a sliding seat 41 fixedly mounted on the upper surface of the annular plate 32, a slider 42 is cooperatively mounted inside the sliding seat 41, limiting rods 43 are fixedly connected to both sides of the outer surface of the slider 42, positioning blocks 44 are slidably connected to both sides of the upper surface of the slider 42, a connecting spring 45 is fixedly connected between the two groups of positioning blocks 44, the moving directions of the positioning blocks 44 and the slider 42 are perpendicular to each other, a plurality of groups of slots 46 are provided on the outer surface of the sliding seat 41, the slots 46 include positions a, b, and c, when the positioning block 44 is located at position a, the limiting rod 43 is inserted into the fixed hole 47 on the surface of the rotating shaft 31; when the positioning block 44 is located at positions b and c, the limiting rod 43 is inserted into the fixed hole 47 on the inner wall of the heating pool 2.

[0047] The annular plate 32 and the heating pool 2 are locked or the annular plate 32 and the driving member are locked by switching the positions of the positioning blocks 44. Specifically, the two groups of positioning blocks 44 are moved toward the middle of the slider 42 so that the positioning blocks 44 are moved out of the slots 46. The positioning blocks 44 no longer interfere with the movement of the slider 42. When the slider 42 is moved to the a position, the positioning blocks 44 are released so that the two groups of positioning blocks 44 are embedded in the slots 46 at the a position. At this time, the limiting rod 43 is inserted into the fixing hole 47 on the surface of the rotating shaft 31, so that the annular plate 32 can be connected to the rotating shaft 31. When the motor 35 drives the rotating shaft 31 to rotate, the annular plate 32 also rotates accordingly, thereby realizing the oscillation of the test tube in the circumferential direction. When the positioning blocks 44 are embedded in the slots 46 at the b position, the limiting rod 43 on one side of the slider 42 is moved out of the rotating shaft 31. The limit rod 43 on the other side of the slider 42 is inserted into the fixing hole 47 on the inner wall of the heating pool 2, so that the annular plate 32 can be locked with the heating pool 2. When the motor 35 drives the rotating shaft 31 to rotate, the rotating shaft 31 will pull the pull rope 36 to move, and the pull rope 36 will drive the jacket 33 to move inside the slide groove 34. At this time, the moving spring 37 is compressed. It should be noted that: in this state, the motor 35 is in a swinging state. When the motor 35 swings back, the compressed moving spring 37 drives the jacket 33 to move back along the slide groove 34. Therefore, when the motor 35 swings back and forth, the jacket 33 can move back and forth along the slide groove 34, thereby realizing the horizontal oscillation of the test tube.

[0048] like Figures 3 to 11 As shown, it also includes the limiting mechanism 5, which includes a sleeve plate 51, which is rotatably sleeved on the outer surface of the annular plate 32, and the upper surface of the annular plate 32 is fixedly connected with a first limiting plate 53 and a second limiting plate 55 at positions on both sides of the slide groove 34. The outer surface of the annular plate 32 is partially recessed inward to form an arc groove, and the sleeve plate 51 is partially located inside the arc groove and forms a rotating pair therein. An arc spring 52 is arranged inside the arc groove, and one end of the arc spring 52 is fixedly connected to the inner wall of the arc groove, and the other end of the arc spring 52 is fixedly connected to the sleeve plate 51. A connecting rope 56 is fixedly connected between the sleeve plate 51 and the slider 42, and the first limiting plate 53 and the second limiting plate 55 are both arranged in an L shape. A limiting groove 54 is opened on the upper surface of the jacket 33, and the first limiting plate 53 and the second limiting plate 55 can be moved into or out of the limiting groove 54.

[0049] By setting the limiting mechanism 5, when the positioning block 44 is located at the a position, it is a circular motion working state, and at this time the first limiting plate 53 is located inside the limiting groove 54, the arc spring 52 is in a stretched state, and the first limiting plate 53 can limit the jacket 33 to prevent the jacket 33 from moving along the slide groove 34 during the circular motion, thereby improving the stability of the jacket 33 during the circular motion; when the positioning block 44 is located at the b position, it is a horizontal motion working state, and at this time when the positioning block 44 switches from the a position to the b position, the connecting rope 56 will relax, and the arc spring 52 drives the sleeve plate 51, the first limiting plate 53 and the second limiting plate 55 to deflect, so that the first limiting plate 53 moves out of the limiting groove 54, and the second limiting plate 55 approaches but does not enter the limiting groove 54. At this time, neither the first limiting plate 53 nor the second limiting plate 55 limits the jacket 33, and the driving member can drive the jacket 33 to move along the slide groove 34.

[0050] The clamping mechanism includes a clamping plate 77, which is configured as a frame-type structure. The clamping plate 77 has a bottom located inside the through groove 76, and the pull rope 36 passes through the inside of the clamping plate 77. A support spring 78 is fixedly connected to the bottom of the through groove 76. The upper end of the support spring 78 is fixedly connected to the bottom of the clamping plate 77. The support spring 78 causes the bottom of the clamping plate 77 to press the pull rope 36. Both sides of the clamping plate 77 pass through the jacket 33 and slide with it. The top of the clamping plate 77 is configured as a wedge-shaped structure and is arranged inside the limiting groove 54.

[0051] When the positioning block 44 is located at the position c, the second limiting plate 55 is located inside the limiting groove 54 and presses down the clamping plate 77 .

[0052] When the positioning block 44 is located at the c position, it is in the vertical movement working state. Specifically, when the positioning block 44 switches from the b position to the c position, the connecting rope 56 will relax, and the arc spring 52 continues to drive the sleeve plate 51, the first limiting plate 53 and the second limiting plate 55 to deflect, so that the second limiting plate 55 moves into the limiting groove 54 and presses down the clamping plate 77. At this time, the second limiting plate 55 restricts the jacket 33, restricting the movement of the jacket 33 along the slide groove 34, and the clamping plate 77 no longer presses the pull rope 36. When the driving part rotates, the fixed seat 71 is pulled up along the cylindrical groove 75 by the pull rope 36. At this time, the telescopic spring 73 is compressed. It should be noted that: in this state, the motor 35 is in a swinging state. When the motor 35 swings back, the compressed telescopic spring 73 drives the fixed seat 71 to move downward. Therefore, when the motor 35 swings back and forth, the fixed seat 71 can move up and down, thereby realizing the oscillation of the test tube in the vertical direction.

[0053] A water injection hole 9 is provided on the upper surface of the heating box 1, and the lower end of the water injection hole 9 is connected to the heating pool 2. A drain pipe 6 is fixedly installed at the bottom of the heating pool 2. The water outlet end of the drain pipe 6 is located outside the heating box 1, and valve bodies are provided inside the drain pipe 6 and the water injection hole 9.

[0054] The valve body in this embodiment can be used in conjunction with any existing type of solenoid valve component to meet the simple control of liquid outflow; in this embodiment, there is no requirement for the type of solenoid valve. At the same time, a button can be set on the outer surface of the heating box 1, and a battery assembly can be set on the heating box 1 or any other part to provide corresponding power for the solenoid valve. Pressing the button can control the switch of the solenoid valve.

[0055] The workflow of the technical solution provided by the present invention is as follows:

[0056] When in use, the annular plate 32 and the heating pool 2 are locked or the annular plate 32 and the driving member are locked by switching the position of the positioning block 44; specifically, the two groups of positioning blocks 44 are moved toward the middle of the slider 42, so that the positioning blocks 44 are moved out of the slots 46, and the positioning blocks 44 no longer interfere with the movement of the slider 42. When the slider 42 is moved to the a position, the positioning blocks 44 are released, so that the two groups of positioning blocks 44 are embedded in the slots 46 at the a position. At this time, the limit rod 43 is inserted into the fixing hole 47 on the surface of the rotating shaft 31, that is, the annular plate 32 can be connected to the rotating shaft 31. When the motor 35 drives the rotating shaft 31 to rotate, the annular plate 32 also rotates accordingly, thereby realizing the oscillation of the test tube in the circumferential direction; when the positioning block 44 is embedded in the slot 46 at the b position When the motor 35 is used to drive the rotating shaft 31 to rotate, the rotating shaft 31 will pull the pull rope 36 to move, and the pull rope 36 will drive the jacket 33 to move inside the slide groove 34. At this time, the moving spring 37 is compressed. It should be noted that: in this state, the motor 35 is in a swinging state. When the motor 35 swings back, the compressed moving spring 37 drives the jacket 33 to move back along the slide groove 34. Therefore, when the motor 35 swings back and forth, the jacket 33 can move back and forth along the slide groove 34, thereby realizing the horizontal oscillation of the test tube.

[0057] When the positioning block 44 is located at the c position, it is in the vertical movement working state. Specifically, when the positioning block 44 switches from the b position to the c position, the connecting rope 56 will relax, and the arc spring 52 continues to drive the sleeve plate 51, the first limiting plate 53 and the second limiting plate 55 to deflect, so that the second limiting plate 55 moves into the limiting groove 54 and presses down the clamping plate 77. At this time, the second limiting plate 55 restricts the jacket 33, restricting the movement of the jacket 33 along the slide groove 34, and the clamping plate 77 no longer presses the pull rope 36. When the driving part rotates, the fixed seat 71 is pulled up along the cylindrical groove 75 by the pull rope 36. At this time, the telescopic spring 73 is compressed. It should be noted that: in this state, the motor 35 is in a swinging state. When the motor 35 swings back, the compressed telescopic spring 73 drives the fixed seat 71 to move downward. Therefore, when the motor 35 swings back and forth, the fixed seat 71 can move up and down, thereby realizing the oscillation of the test tube in the vertical direction.

[0058] The present invention covers any substitution, modification, equivalent method and scheme made on the essence and scope of the present invention. In order to make the public have a thorough understanding of the present invention, specific details are described in detail in the following preferred embodiments of the present invention, but those skilled in the art can fully understand the present invention without the description of these details. In addition, in order to avoid unnecessary confusion about the essence of the present invention, well-known methods, processes, procedures, components and circuits are not described in detail.

[0059] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principle of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A cell water bath shaking culture device, comprising a heating box, characterized in that: The upper surface of the heating box is provided with a heating pool, and a shaking mechanism is arranged inside the heating pool. The shaking mechanism includes an annular plate and a driving member arranged at the center of the bottom surface of the heating pool. The annular plate is sleeved with the top of the driving member. The surface of the annular plate is provided with multiple groups of slide grooves, and the slide grooves are all provided with jackets, and the jackets are used to clamp the test tubes. The upper surface of the annular plate is provided with a locking mechanism; When the annular plate is connected to the heating pool, the driving member can make the jacket move back and forth inside the slide groove; when the annular plate is connected to the driving member, the driving member can drive the jacket to perform circular motion.

2. The cell water bath shaking culture device according to claim 1, characterized in that: The driving member includes a motor, the output shaft of the motor is fixedly connected to a rotating shaft, the upper end of the rotating shaft is arranged as an I-shaped structure, the annular plate is rotatably sleeved on the outer surface of the recessed portion of the I-shaped structure of the rotating shaft, a pull rope is arranged between the sleeve and the rotating shaft, the pull rope passes through the inner wall of the annular plate, a movable spring is arranged inside the slide groove, one end of the movable spring is fixedly connected to the sleeve, and the other end of the movable spring is fixedly connected to the inner surface of the slide groove.

3. The cell water bath shaking culture device according to claim 2, characterized in that: The locking mechanism includes a sliding seat fixedly mounted on the upper surface of the annular plate, a sliding block is cooperatively mounted inside the sliding seat, limiting rods are fixedly connected on both sides of the outer surface of the sliding block, positioning blocks are slidably connected on both sides of the upper surface of the sliding block, a connecting spring is fixedly connected between the two groups of the positioning blocks, the moving directions of the positioning blocks and the sliding block are perpendicular to each other, a plurality of groups of slots are provided on the outer surface of the sliding seat, the slots include positions a, b, and c, when the positioning block is located at position a, the limiting rod is inserted into the fixed hole on the surface of the rotating shaft; when the positioning block is located at positions b and c, the limiting rod is inserted into the fixed hole on the inner wall of the heating pool.

4. The cell water bath shaking culture device according to claim 3, characterized in that: It also includes a limiting mechanism, which includes a sleeve plate, which is rotatably sleeved on the outer surface of the annular plate, and the upper surface of the annular plate is fixedly connected with a first limiting plate and a second limiting plate at positions on both sides of the slide groove, the outer surface of the annular plate is partially recessed inward to form an arc groove, and the sleeve plate is partially located inside the arc groove and forms a rotating pair therein, an arc spring is arranged inside the arc groove, one end of the arc spring is fixedly connected to the inner wall of the arc groove, and the other end of the arc spring is fixedly connected to the sleeve plate, a connecting rope is fixedly connected between the sleeve plate and the slider, the first limiting plate and the second limiting plate are both arranged in an L shape, and limiting grooves are provided on the upper surface of the jacket.

5. The cell water bath shaking culture device according to claim 4, characterized in that: It also includes a vertical movement mechanism, which includes a cylindrical groove opened on one side of the lower surface of the jacket, a guide rod is installed inside the cylindrical groove, the lower end of the guide rod is fixedly connected to a fixing seat, a rubber pad is fixedly installed on the inner surface of the fixing seat, a telescopic spring is sleeved on the outer surface of the guide rod, the upper end of the telescopic spring is fixedly connected to the jacket, the lower end of the telescopic spring is fixedly connected to the fixing seat, a through groove is opened inside the jacket, one end of the pull rope passes through the through groove and is fixedly connected to the fixing seat, a clamping mechanism is arranged inside the through groove, and the clamping mechanism is used to fix the pull rope.

6. The cell water bath shaking culture device according to claim 5, characterized in that: The clamping mechanism includes a clamping plate, which is configured as a frame-type structure. The bottom of the clamping plate is located inside a through groove, and the pull rope passes through the inside of the clamping plate. A support spring is fixedly connected to the bottom of the through groove, and the upper end of the support spring is fixedly connected to the bottom of the clamping plate. The support spring presses the pull rope tightly at the bottom of the clamping plate. Both sides of the clamping plate pass through the jacket and slide with it, and the top of the clamping plate is configured as a wedge-shaped structure and is arranged inside the limiting groove.

7. The cell water bath shaking culture device according to claim 6, characterized in that: When the positioning block is located at position a, the first limiting plate is located inside the limiting groove; when the positioning block is located at position b, neither the first limiting plate nor the second limiting plate is located inside the limiting groove; when the positioning block is located at position c, the second limiting plate is located inside the limiting groove and presses down the clamp.

8. The cell water bath shaking culture device according to claim 2, characterized in that: The slide grooves are distributed in a ring array with the rotating shaft as a base point, and the slide grooves are all arranged along the radial direction of the ring plate.

9. The cell water bath shaking culture device according to claim 2, characterized in that: An inner cavity is formed at the bottom of the heating box, and the motor is fixedly installed inside the inner cavity.

10. The cell water bath shaking culture device according to claim 2, characterized in that: A water injection hole is provided on the upper surface of the heating box, and the lower end of the water injection hole is connected to the heating pool. A drain pipe is fixedly installed at the bottom of the heating pool, and the water outlet end of the drain pipe is located outside the heating box. Valve bodies are provided inside the drain pipe and the water injection hole.

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