A cell water bath shaking culture device

Abstract

The present invention provides a cell water bath shaking culture device, which belongs to the field of biological culture technology. It includes a heating box, a heating pool is provided on the upper surface of the heating box, a shaking mechanism is provided inside the heating pool, and the shaking mechanism includes an annular plate and a driving member provided at the center of the bottom surface of the heating pool, and the annular plate is sleeved on the top of the driving member. The present invention sets a locking mechanism. When the locking mechanism connects the annular plate with the driving member, the driving member can drive the annular plate, the jacket, the fixed seat and the test tube to rotate, thereby realizing oscillation of the test tube in the circumferential direction; when the locking mechanism connects the annular plate with the heating pool, the driving member can drive the jacket to move along the slide groove, thereby realizing horizontal oscillation of the test tube; in addition, by setting a vertical moving mechanism, vertical oscillation of the test tube can be realized; therefore, the present invention has various oscillation modes, and different oscillation modes can be selected according to actual cell culture needs to meet the culture of different cells.

Description

Technical Field

[0001] The present invention relates to the field of biological culture technology, and more particularly to a cell water bath shaking culture device. Background Art

[0002] Oscillating culture, also known as suspension culture, involves inoculating microbial cells into a liquid medium and placing them on a shaker or oscillator for continuous agitation. Widely used for strain screening and microbial expansion, it is a common method in microbial physiology, biochemistry, fermentation, and other life science research fields.

[0003] The main purpose of shaking culture is to simulate the environment in the body, provide a more constant and stable culture condition, promote cell growth and division, and facilitate large-scale cell culture. Through shaking culture, we can better understand the growth patterns and metabolic processes of cells, providing convenience and avenues for cell research and application. However, existing water bath shakers can only perform horizontal oscillation or vertical oscillation mixing of reagents, resulting in a single oscillation mode that cannot meet various usage requirements. For this reason, a cell water bath shaking culture device is proposed. Summary of the Invention

[0004] In view of the problem that existing water bath shakers can only oscillate horizontally or can only oscillate and mix reagents vertically, resulting in a single oscillation mode and being unable to meet various usage requirements, the purpose of the present invention is to provide a cell water bath shaking culture device.

[0005] In order to solve the above problems, the present invention adopts the following technical solutions:

[0006] A cell water bath shaking culture device comprises a heating box, a heating pool is provided on the upper surface of the heating box, a shaking mechanism is provided inside the heating pool, the shaking mechanism comprises an annular plate and a driving member provided at the center of the bottom surface of the heating pool, the annular plate is sleeved on the top of the driving member, a plurality of groups of chutes are provided on the surface of the annular plate, each of the chutes is provided with a jacket, the jacket is used to clamp a test tube, and a locking mechanism is provided on the upper surface of the annular plate, the locking mechanism can connect the annular plate to the heating pool or the driving member;

[0007] When the annular plate is connected to the heating pool, the driving member can make the jacket move back and forth inside the chute; when the annular plate is connected to the driving member, the driving member can drive the jacket to perform circular motion.

[0008] Optionally, the driving member includes a motor, the output shaft of the motor is fixedly connected to a rotating shaft, the upper end of the rotating shaft is set as an I-shaped structure, the annular plate is rotatably sleeved on the outer surface of the recessed portion of the I-shaped structure of the rotating shaft, a pull rope is provided between the sleeve and the rotating shaft, the pull rope passes through the inner wall of the annular plate, and a movable spring is provided inside the slide groove, one end of the movable spring is fixedly connected to the sleeve, and the other end of the movable spring is fixedly connected to the inner surface of the slide groove.

[0009] Optionally, the locking mechanism includes a sliding seat fixedly mounted on the upper surface of the annular plate, a slider is cooperatively mounted inside the sliding seat, limiting rods are fixedly connected on both sides of the outer surface of the slider, positioning blocks are slidably connected on both sides of the upper surface of the slider, a connecting spring is fixedly connected between the two groups of positioning blocks, the positioning blocks and the moving directions of the slider are perpendicular to each other, a plurality of groups of slots are provided on the outer surface of the sliding seat, and the slots include positions a, b, and c. When the positioning block is at position a, the limiting rod is inserted into the fixed hole on the surface of the rotating shaft; when the positioning block is at positions b and c, the limiting rod is inserted into the fixed hole on the inner wall of the heating pool.

[0010] Optionally, it also includes a limiting mechanism, which includes a sleeve plate, which is rotatably sleeved on the outer surface of the annular plate, and the upper surface of the annular plate is fixedly connected to the first limiting plate and the second limiting plate at positions on both sides of the slide groove, the outer surface of the annular plate is partially recessed inward to form an arc groove, the sleeve plate is partially located inside the arc groove and forms a rotating pair therein, an arc spring is provided inside the arc groove, one end of the arc spring is fixedly connected to the inner wall of the arc groove, and the other end of the arc spring is fixedly connected to the sleeve plate, and a connecting rope is fixedly connected between the sleeve plate and the slider, the first limiting plate and the second limiting plate are both configured to be L-shaped, and the upper surface of the jacket is provided with a limiting groove, and the first limiting plate and the second limiting plate can both move in or out of the limiting groove.

[0011] Optionally, it also includes a vertical moving mechanism, which includes a cylindrical groove opened on one side of the lower surface of the jacket, a guide rod is installed inside the cylindrical groove, the lower end of the guide rod is fixedly connected to a fixing seat, a rubber pad is fixedly installed on the inner surface of the fixing seat, and a telescopic spring is sleeved on the outer surface of the guide rod, the upper end of the telescopic spring is fixedly connected to the jacket, and the lower end of the telescopic spring is fixedly connected to the fixing seat, a through groove is opened inside the jacket, one end of the pull rope passes through the through groove and is fixedly connected to the fixing seat, and a clamping mechanism is provided inside the through groove, and the clamping mechanism is used to fix the pull rope.

[0012] Optionally, the clamping mechanism includes a splint, which is configured as a frame-type structure, the splint, and the bottom of the splint is located inside the through groove, the pull rope passes through the inside of the splint, and a support spring is fixedly connected to the bottom of the through groove, and the upper end of the support spring is fixedly connected to the bottom of the splint. The support spring presses the pull rope at the bottom of the splint, and both sides of the splint pass through the jacket and slide with it, and the top of the splint is configured as a wedge-shaped structure and is arranged inside the limiting groove.

[0013] Optionally, when the positioning block is located at position a, the first limiting plate is located inside the limiting groove; when the positioning block is located at position b, neither the first limiting plate nor the second limiting plate is located inside the limiting groove; when the positioning block is located at position c, the second limiting plate is located inside the limiting groove and presses down the splint.

[0014] Optionally, the sliding grooves are distributed in an annular array with the rotation axis as a base point, and the sliding grooves are all arranged along the radial direction of the annular plate.

[0015] Optionally, an inner cavity is opened at the bottom of the heating box, and the motor is fixedly installed inside the inner cavity.

[0016] Optionally, a water injection hole is opened on the upper surface of the heating box, the lower end of the water injection hole is connected to the heating pool, a drain pipe is fixedly installed at the bottom of the heating pool, the water outlet end of the drain pipe is located outside the heating box, and valve bodies are provided inside the drain pipe and the water injection hole.

[0017] Compared with the prior art, the above technical solution provided by the present invention has at least the following beneficial effects:

[0018] In the above scheme, by setting a locking mechanism, when the locking mechanism connects the annular plate to the driving member, the driving member can drive the annular plate, the jacket, the fixing seat and the test tube to rotate, thereby realizing the circumferential oscillation of the test tube; when the locking mechanism connects the annular plate to the heating pool, the driving member can drive the jacket to move along the slide groove, thereby realizing the horizontal oscillation of the test tube; in addition, by setting a vertical moving mechanism, the vertical oscillation of the test tube can be realized; therefore, the present invention has various oscillation modes, and different oscillation modes can be selected according to actual cell culture needs to meet the culture of different cells.

[0019] By setting a limiting mechanism, when the positioning block is at position a, it is in the circular motion working state, and at this time the first limiting plate is located inside the limiting groove, the arc spring is in a stretched state, and the first limiting plate can limit the sleeve to prevent the sleeve from moving along the slide groove during circular motion, thereby improving the stability of the sleeve during circular motion. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] The accompanying drawings, which are incorporated herein and constitute a part of the specification, illustrate embodiments of the invention and, together with the description, further serve to explain the principles of the invention and to enable one skilled in the art to make and use the invention.

[0021] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0022] Figure 2 is a cross-sectional view of the present invention;

[0023] Figure 3 It is a structural diagram of the shaking mechanism of the present invention;

[0024] Figure 4 It is a structural schematic diagram of the vertical moving mechanism of the present invention;

[0025] Figure 5 It is a structural schematic diagram of the driving member of the present invention;

[0026] Figure 6 It is a structural schematic diagram of the locking mechanism of the present invention;

[0027] Figure 7 For the present invention Figure 6 Enlarged view of point A in the middle;

[0028] Figure 8 Schematic diagram of the structure of the limiting mechanism of the present invention;

[0029] Figure 9 This is a schematic diagram of the structure when the positioning block of the present invention is located at site a;

[0030] Figure 10 This is a schematic diagram of the structure when the positioning block of the present invention is located at site b;

[0031] Figure 11 This is a schematic structural diagram of the positioning block of the present invention when it is located at the c site.

[0032] [Reference Signs]

[0033] 1. Heating box; 2. Heating pool; 3. Shaking mechanism; 31. Rotating shaft; 32. Annular plate; 33. Jacket; 34. Slide; 35. Motor; 36. Pull rope; 37. Moving spring; 4. Locking mechanism; 41. Slide seat; 42. Sliding block; 43. Limit rod; 44. Positioning block; 45. Connecting spring; 46. Slot; 47. Fixing hole; 5. Limiting mechanism; 51. Sleeve plate; 52. Arc spring; 53. First limiting plate; 54. Limiting groove; 55. Second limiting plate; 56. Connecting rope; 6. Drain pipe; 7. Vertical moving mechanism; 71. Fixed seat; 72. Rubber pad; 73. Telescopic spring; 74. Guide rod; 75. Cylindrical groove; 76. Through groove; 77. Clamp; 78. Support spring; 8. Inner cavity; 9. Water injection hole.

[0034] As shown in the figure, in order to clearly implement the structure of the embodiment of the present invention, specific structures and devices are marked in the figure, but this is only for illustrative purposes and is not intended to limit the present invention to the specific structure, device and environment. According to specific needs, ordinary technicians in this field can adjust or modify these devices and environments. DETAILED DESCRIPTION

[0035] The following is a detailed description of a cell water bath shaking culture device provided by the present invention, with reference to the accompanying drawings and specific embodiments. It is also noted that, in order to make the embodiments more detailed, the following embodiments are best and preferred embodiments, and those skilled in the art may also adopt other alternatives to implement some known technologies; and the accompanying drawings are only for the purpose of describing the embodiments in more detail, and are not intended to specifically limit the present invention.

[0036] It should be noted that references in the specification to "one embodiment," "an embodiment," "exemplary embodiments," "some embodiments," etc. indicate that the described embodiments may include specific features, structures, or characteristics, but not necessarily every embodiment will include such specific features, structures, or characteristics. Furthermore, when specific features, structures, or characteristics are described in conjunction with an embodiment, it is within the knowledge of persons skilled in the relevant art to implement such features, structures, or characteristics in conjunction with other embodiments (whether or not explicitly described).

[0037] In general, terms can be understood, at least in part, from their use in context. For example, depending at least in part on the context, the term "one or more" as used herein can be used to describe any feature, structure, or characteristic in the singular sense, or can be used to describe a combination of features, structures, or characteristics in the plural sense. Additionally, the term "based on" can be understood as not necessarily intended to convey an exclusive set of factors, but can instead, depending at least in part on the context, allow for the presence of other factors that are not necessarily explicitly described.

[0038] It will be understood that the meanings of “on,” “over,” and “above” in the present invention should be interpreted in the broadest manner, so that “on” means not only “directly on” something but also includes the meaning of being “on” something with intervening features or layers, and “on” or “above” means not only “on” or “above” something but also includes the meaning of being “on” or “above” something with no intervening features or layers.

[0039] Additionally, spatially relative terms such as "below," "beneath," "lower," "above," and "upper" may be used herein for descriptive convenience to describe the relationship of one element or feature to another element or features, as illustrated in the accompanying drawings. Spatially relative terms are intended to encompass different orientations of the device in use or operation in addition to the orientation depicted in the accompanying drawings. The device may be oriented in other ways, and the spatially relative descriptors used herein should be similarly interpreted accordingly.

[0040] like Figures 1 to 4 As shown, an embodiment of the present invention provides a cell water bath shaking culture device, including a heating box 1, a heating pool 2 is provided on the upper surface of the heating box 1, a shaking mechanism 3 is provided inside the heating pool 2, the shaking mechanism 3 includes an annular plate 32 and a driving member arranged at the center of the bottom surface of the heating pool 2, the annular plate 32 is sleeved on the top of the driving member, a plurality of groups of slide grooves 34 are provided on the surface of the annular plate 32, and a jacket 33 is provided inside the slide grooves 34, the jacket 33 is used to clamp the test tube, and a locking mechanism 4 is provided on the upper surface of the annular plate 32, which can connect the annular plate 32 to the heating pool 2 or the driving member; the slide grooves 34 are distributed in an annular array with the rotating shaft 31 as the base point, and the slide grooves 34 are all arranged along the radial direction of the annular plate 32.

[0041] When the annular plate 32 is connected to the heating pool 2, the driving member can make the jacket 33 move back and forth inside the slide groove 34; when the annular plate 32 is connected to the driving member, the driving member can drive the jacket 33 to perform circular motion.

[0042] It also includes a vertical moving mechanism 7, which includes a cylindrical groove 75 opened on one side of the lower surface of the jacket 33, and a guide rod 74 is installed inside the cylindrical groove 75. The lower end of the guide rod 74 is fixedly connected to the fixing seat 71, and a rubber pad 72 is fixedly installed on the inner surface of the fixing seat 71. The outer surface of the guide rod 74 is sleeved with a telescopic spring 73, the upper end of the telescopic spring 73 is fixedly connected to the jacket 33, and the lower end of the telescopic spring 73 is fixedly connected to the fixing seat 71. A through groove 76 is opened inside the jacket 33, and one end of the pull rope 36 passes through the through groove 76 and is fixedly connected to the fixing seat 71. A clamping mechanism is provided inside the through groove 76, and the clamping mechanism is used to fix the pull rope 36.

[0043] In this embodiment, the fixing seat 71 and the jacket 33 are used to clamp the test tube containing the cell solution, wherein a rubber pad 72 is provided inside the fixing seat 71. When the bottom of the test tube is inserted into the fixing seat 71, there is an interference fit between the test tube and the fixing seat 71. The inner diameter of the jacket 33 should actually be slightly larger than the size of the test tube so that the test tube can move up and down along the jacket 33.

[0044] By setting the locking mechanism 4, when the locking mechanism 4 connects the annular plate 32 with the driving member, the driving member can drive the annular plate 32, the jacket 33, the fixing seat 71 and the test tube to rotate, thereby realizing the circumferential oscillation of the test tube; when the locking mechanism 4 connects the annular plate 32 with the heating pool 2, the driving member can drive the jacket 33 to move along the slide groove 34, thereby realizing the horizontal oscillation of the test tube; in addition, by setting the vertical moving mechanism 7, the vertical oscillation of the test tube can be realized; therefore, the present invention has various oscillation modes, and different oscillation modes can be selected according to actual cell culture needs to meet the culture of different cells.

[0045] like Figure 2 、 Figure 5 as well as Figure 8 As shown, the driving part includes a motor 35, the output shaft of the motor 35 is fixedly connected to the rotating shaft 31, the upper end of the rotating shaft 31 is set as an I-shaped structure, the annular plate 32 is rotatably sleeved on the outer surface of the recessed portion of the I-shaped structure of the rotating shaft 31, and a pull rope 36 is provided between the jacket 33 and the rotating shaft 31. The pull rope 36 passes through the inner wall of the annular plate 32, and a movable spring 37 is provided inside the slide groove 34. One end of the movable spring 37 is fixedly connected to the jacket 33, and the other end of the movable spring 37 is fixedly connected to the inner surface of the slide groove 34. An inner cavity 8 is opened at the bottom of the heating box 1, and the motor 35 is fixedly installed inside the inner cavity 8.

[0046] like Figures 4 to 7 As shown, the locking mechanism 4 includes a slide 41 fixedly mounted on the upper surface of the annular plate 32, a slider 42 is fitted inside the slide 41, and limiting rods 43 are fixedly connected on both sides of the outer surface of the slider 42, and positioning blocks 44 are slidably connected on both sides of the upper surface of the slider 42, and a connecting spring 45 is fixedly connected between the two groups of positioning blocks 44, and the moving directions of the positioning blocks 44 and the slider 42 are perpendicular to each other, and a plurality of groups of slots 46 are provided on the outer surface of the slide 41, and the slots 46 include positions a, b, and c. When the positioning block 44 is at position a, the limiting rod 43 is inserted into the fixed hole 47 on the surface of the rotating shaft 31; when the positioning block 44 is at positions b or c, the limiting rod 43 is inserted into the fixed hole 47 on the inner wall of the heating pool 2.

[0047] The annular plate 32 is locked to the heating pool 2 or to the driving member by switching the position of the positioning blocks 44. Specifically, the two groups of positioning blocks 44 are moved toward the middle of the slider 42 so that the positioning blocks 44 are removed from the inside of the slots 46. The positioning blocks 44 no longer interfere with the movement of the slider 42. When the slider 42 is moved to the a position, the positioning blocks 44 are released so that the two groups of positioning blocks 44 are embedded in the slots 46 at the a position. At this time, the limiting rod 43 is inserted into the fixing hole 47 on the surface of the rotating shaft 31, so that the annular plate 32 can be connected to the rotating shaft 31. When the motor 35 drives the rotating shaft 31 to rotate, the annular plate 32 also rotates accordingly, thereby realizing the oscillation of the test tube in the circumferential direction. When the positioning blocks 44 are embedded in the slots 46 at the b position, the limiting rod 43 on one side of the slider 42 is removed from the outside of the rotating shaft 31. The limit rod 43 on the other side of the slider 42 is inserted into the fixed hole 47 on the inner wall of the heating pool 2, so that the annular plate 32 can be locked with the heating pool 2. When the motor 35 drives the rotating shaft 31 to rotate, the rotating shaft 31 will pull the pull rope 36 to move, and the pull rope 36 will drive the jacket 33 to move inside the slide groove 34. At this time, the moving spring 37 is compressed. It should be noted that: in this state, the motor 35 is in a swinging state. When the motor 35 swings back, the compressed moving spring 37 drives the jacket 33 to move back along the slide groove 34. Therefore, when the motor 35 swings back and forth, the jacket 33 can move back and forth along the slide groove 34, thereby realizing the horizontal oscillation of the test tube.

[0048] like Figures 3 to 11 As shown, it also includes the limiting mechanism 5, which includes a sleeve plate 51, which is rotatably sleeved on the outer surface of the annular plate 32, and the upper surface of the annular plate 32 is fixedly connected with a first limiting plate 53 and a second limiting plate 55 at positions on both sides of the slide groove 34. The outer surface of the annular plate 32 is partially recessed inward to form an arc groove, and the sleeve plate 51 is partially located inside the arc groove and forms a rotating pair therein. An arc spring 52 is provided inside the arc groove, and one end of the arc spring 52 is fixedly connected to the inner wall of the arc groove, and the other end of the arc spring 52 is fixedly connected to the sleeve plate 51. A connecting rope 56 is fixedly connected between the sleeve plate 51 and the slider 42, and the first limiting plate 53 and the second limiting plate 55 are both configured to be L-shaped. A limiting groove 54 is provided on the upper surface of the jacket 33, and the first limiting plate 53 and the second limiting plate 55 can be moved into or out of the limiting groove 54.

[0049] By setting the limiting mechanism 5, when the positioning block 44 is at position a, it is a circular motion working state, and at this time the first limiting plate 53 is located inside the limiting groove 54, the arc spring 52 is in a stretched state, and the first limiting plate 53 can limit the sleeve 33, preventing the sleeve 33 from moving along the slide groove 34 during circular motion, thereby improving the stability of the sleeve 33 during circular motion; when the positioning block 44 is at position b, it is a horizontal motion working state, and at this time when the positioning block 44 switches from position a to position b, the connecting rope 56 will relax, and the arc spring 52 drives the sleeve plate 51, the first limiting plate 53 and the second limiting plate 55 to deflect, so that the first limiting plate 53 moves out of the limiting groove 54, and the second limiting plate 55 approaches but does not enter the limiting groove 54. At this time, neither the first limiting plate 53 nor the second limiting plate 55 limits the sleeve 33, and the driving member can drive the sleeve 33 to move along the slide groove 34.

[0050] The clamping mechanism includes a clamping plate 77, which is configured as a frame-type structure. The bottom of the clamping plate 77 is located inside the through groove 76, and the pull rope 36 passes through the inside of the clamping plate 77. A support spring 78 is fixedly connected to the bottom of the through groove 76. The upper end of the support spring 78 is fixedly connected to the bottom of the clamping plate 77. The support spring 78 presses the pull rope 36 at the bottom of the clamping plate 77. Both sides of the clamping plate 77 pass through the jacket 33 and slide with it. The top of the clamping plate 77 is configured as a wedge-shaped structure and is arranged inside the limiting groove 54.

[0051] When the positioning block 44 is located at position c, the second limiting plate 55 is located inside the limiting groove 54 and presses down the clamping plate 77 .

[0052] When the positioning block 44 is at the C position, it is in the vertical movement working state. Specifically, when the positioning block 44 switches from the B position to the C position, the connecting rope 56 will relax, and the arc spring 52 continues to drive the sleeve plate 51, the first limiting plate 53 and the second limiting plate 55 to deflect, so that the second limiting plate 55 moves into the limiting groove 54 and presses the clamping plate 77 downward. At this time, the second limiting plate 55 restricts the jacket 33, restricting the jacket 33 from moving along the slide groove 34, and the clamping plate 77 no longer presses the pull rope 36. When the driving part rotates, the pull rope 36 pulls the fixed seat 71 to move up along the cylindrical groove 75. At this time, the telescopic spring 73 is compressed. It should be noted that: in this state, the motor 35 is in a swinging state. When the motor 35 swings back, the compressed telescopic spring 73 drives the fixed seat 71 to move downward. Therefore, when the motor 35 swings back and forth, the fixed seat 71 can move up and down, thereby realizing the vertical oscillation of the test tube.

[0053] A water injection hole 9 is provided on the upper surface of the heating box 1, and the lower end of the water injection hole 9 is connected to the heating pool 2. A drain pipe 6 is fixedly installed at the bottom of the heating pool 2. The water outlet end of the drain pipe 6 is located outside the heating box 1, and valve bodies are provided inside the drain pipe 6 and the water injection hole 9.

[0054] The valve body in this embodiment can be used in conjunction with any existing type of solenoid valve component to meet the simple liquid outflow control; in this embodiment, there is no requirement for the model of the solenoid valve. At the same time, a button can be set on the outer surface of the heating box 1, and a battery assembly can be set on the heating box 1 or any other part to provide corresponding power for the solenoid valve. Pressing the button can control the switch of the solenoid valve.

[0055] The workflow of the technical solution provided by the present invention is as follows:

[0056] When in use, the annular plate 32 and the heating pool 2 or the annular plate 32 and the driving member are locked by switching the position of the positioning block 44; specifically, the two groups of positioning blocks 44 are moved toward the middle of the slider 42, so that the positioning blocks 44 are moved out of the slots 46, and the positioning blocks 44 no longer interfere with the movement of the slider 42. When the slider 42 is moved to the a position, the positioning blocks 44 are released, so that the two groups of positioning blocks 44 are embedded in the slots 46 at the a position. At this time, the limit rod 43 is inserted into the fixing hole 47 on the surface of the rotating shaft 31, that is, the annular plate 32 can be connected to the rotating shaft 31. When the motor 35 drives the rotating shaft 31 to rotate, the annular plate 32 also rotates accordingly, thereby realizing the oscillation of the test tube in the circumferential direction; when the positioning block 44 is embedded in the slot 46 at the b position When the motor 35 is used to drive the rotating shaft 31 to rotate, the rotating shaft 31 will pull the pull rope 36 to move, and the pull rope 36 will drive the jacket 33 to move inside the slide groove 34. At this time, the moving spring 37 is compressed. It should be noted that: in this state, the motor 35 is in a swinging state. When the motor 35 swings back, the compressed moving spring 37 drives the jacket 33 to move back along the slide groove 34. Therefore, when the motor 35 swings back and forth, the jacket 33 can move back and forth along the slide groove 34, thereby realizing the horizontal oscillation of the test tube.

[0057] When the positioning block 44 is at the C position, it is in the vertical movement working state. Specifically, when the positioning block 44 switches from the B position to the C position, the connecting rope 56 will relax, and the arc spring 52 continues to drive the sleeve plate 51, the first limiting plate 53 and the second limiting plate 55 to deflect, so that the second limiting plate 55 moves into the limiting groove 54 and presses the clamping plate 77 downward. At this time, the second limiting plate 55 restricts the jacket 33, restricting the jacket 33 from moving along the slide groove 34, and the clamping plate 77 no longer presses the pull rope 36. When the driving part rotates, the pull rope 36 pulls the fixed seat 71 to move up along the cylindrical groove 75. At this time, the telescopic spring 73 is compressed. It should be noted that: in this state, the motor 35 is in a swinging state. When the motor 35 swings back, the compressed telescopic spring 73 drives the fixed seat 71 to move downward. Therefore, when the motor 35 swings back and forth, the fixed seat 71 can move up and down, thereby realizing the vertical oscillation of the test tube.

[0058] The present invention encompasses any alternatives, modifications, equivalents, and solutions that fall within the spirit and scope of the present invention. To provide a thorough understanding of the present invention, specific details are described in detail below in connection with the preferred embodiments of the present invention, but those skilled in the art will be able to fully understand the present invention without these detailed descriptions. Furthermore, to avoid unnecessary confusion regarding the essence of the present invention, well-known methods, processes, procedures, components, and circuits have not been described in detail.

[0059] The above is only a preferred embodiment of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as within the scope of protection of the present invention.

Claims

1. A cell water bath shaking culture device, comprising a heating box, characterized in that: A heating pool is provided on the upper surface of the heating box, and a shaking mechanism is provided inside the heating pool. The shaking mechanism includes an annular plate and a driving member provided at the center of the bottom surface of the heating pool. The annular plate is sleeved on the top of the driving member. A plurality of chutes are provided on the surface of the annular plate, and jackets are provided inside the chutes. The jackets are used to clamp the test tubes. A locking mechanism is provided on the upper surface of the annular plate. When the annular plate is connected to the heating pool, the driving member can move the jacket back and forth inside the chute; when the annular plate is connected to the driving member, the driving member can drive the jacket to perform circular motion; The driving member includes a motor, the output shaft of the motor is fixedly connected to a rotating shaft, the upper end of the rotating shaft is set as an I-shaped structure, the annular plate is rotatably sleeved on the outer surface of the recessed portion of the rotating shaft I-shaped structure, a pull rope is provided between the jacket and the rotating shaft, the pull rope passes through the inner wall of the annular plate, and a movable spring is provided inside the chute, one end of the movable spring is fixedly connected to the jacket, and the other end of the movable spring is fixedly connected to the inner surface of the chute; The locking mechanism includes a sliding seat fixedly mounted on the upper surface of the annular plate, a sliding block is cooperatedly installed inside the sliding seat, limiting rods are fixedly connected on both sides of the outer surface of the sliding block, positioning blocks are slidably connected on both sides of the upper surface of the sliding block, a connecting spring is fixedly connected between the two groups of positioning blocks, the moving directions of the positioning blocks and the sliding block are perpendicular to each other, and multiple groups of slots are provided on the outer surface of the sliding seat, and the slots include positions a, b, and c. When the positioning block is at position a, the limiting rod is inserted into the fixed hole on the surface of the rotating shaft; when the positioning block is at positions b and c, the limiting rod is inserted into the fixed hole on the inner wall of the heating pool.

2. The cell water bath shaking culture device according to claim 1, characterized in that: The cam is secured to the upper edge of the sliding groove and is adapted to engage with the second limit plate when the cam is engaged with the first limit plate and the second limit plate when the cam is engaged with the first limit plate.

3. The cell water bath shaking culture device according to claim 2, characterized in that: It also includes a vertical moving mechanism, which includes a cylindrical groove opened on one side of the lower surface of the jacket, a guide rod is installed inside the cylindrical groove, the lower end of the guide rod is fixedly connected to a fixing seat, a rubber pad is fixedly installed on the inner surface of the fixing seat, and a telescopic spring is sleeved on the outer surface of the guide rod, the upper end of the telescopic spring is fixedly connected to the jacket, and the lower end of the telescopic spring is fixedly connected to the fixing seat, a through groove is opened inside the jacket, one end of the pull rope passes through the through groove and is fixedly connected to the fixing seat, and a clamping mechanism is provided inside the through groove, and the clamping mechanism is used to fix the pull rope.

4. The cell water bath shaking culture device according to claim 3, characterized in that: The clamping mechanism includes a splint, which is configured as a frame-type structure. The splint and the bottom of the splint are located inside the through groove. The pull rope passes through the splint. The bottom of the through groove is fixedly connected to a support spring. The upper end of the support spring is fixedly connected to the bottom of the splint. The support spring presses the pull rope tightly at the bottom of the splint. Both sides of the splint pass through the jacket and slide with it. The top of the splint is configured as a wedge-shaped structure and is arranged inside the limiting groove.

5. The cell water bath shaking culture device according to claim 4, characterized in that: When the positioning block is at position a, the first limiting plate is located inside the limiting groove; when the positioning block is at position b, neither the first limiting plate nor the second limiting plate is located inside the limiting groove; when the positioning block is at position c, the second limiting plate is located inside the limiting groove and presses down the splint.

6. The cell water bath shaking culture device according to claim 1, characterized in that: The sliding grooves are distributed in an annular array with the rotating shaft as a base point, and the sliding grooves are all arranged along the radial direction of the annular plate.

7. The cell water bath shaking culture device according to claim 1, characterized in that: An inner cavity is formed at the bottom of the heating box, and the motor is fixedly installed inside the inner cavity.

8. The cell water bath shaking culture device according to claim 1, characterized in that: A water injection hole is provided on the upper surface of the heating box, the lower end of the water injection hole is connected to the heating pool, a drain pipe is fixedly installed at the bottom of the heating pool, the water outlet end of the drain pipe is located outside the heating box, and valve bodies are provided inside the drain pipe and the water injection hole.

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