Moso bamboo root pressure measuring method and measuring device

Abstract

The invention belongs to the technical field of plant moisture physiological research, and relates to a moso bamboo root pressure measuring method and measuring device.The moso bamboo root pressure measuring method comprises the steps that branches or rods of moso bamboo to be measured are cut off; filling the cut cross section with distilled water; one end of a sleeve is fixed to the moso bamboo, and the cross section is located in the sleeve; filling the casing pipe with distilled water through the other end of the casing pipe, and connecting the other end of the casing pipe with a hose filled with the distilled water after the casing pipe is filled with the distilled water; the other end of the hose is connected with an electronic pressure sensor through a three-way valve, and root pressure data of the moso bamboo is read through a data collector connected with the electronic pressure sensor. According to the invention, sleeves with different calibers can be replaced according to the sizes of bamboos, and root pressure measurement can be carried out on bamboos with different diameters; according to the invention, only a section of short silica gel hose is used at the pressure sensor, so that the phenomenon of inaccurate pressure measurement caused by bending of the silica gel hose in a field environment is avoided.

Description

Technical Field

[0001] The invention relates to a method and a device for measuring root pressure of moso bamboo, belonging to the technical field of plant water physiology research. Background Art

[0002] The water and nutrients required for plant growth are mainly absorbed from the soil through the root system and transported upward to various organs through the xylem. Root pressure and transpiration pull are the main driving forces for the transport of water and nutrients against gravity. Root pressure is a physiological process in which plants consume energy and actively absorb ions, and water moves upward along the xylem with the concentration difference. When the plant is too tall, the transpiration pull is limited in the water transport process. When the transpiration pull exceeds the tensile strength of the water column in the xylem, the water column will break, forming bubbles and causing embolism. Root pressure can clear gas embolism in the xylem, so that water can be transported normally in the xylem. Therefore, it is necessary to measure root pressure in the research of plant water physiology, drought resistance, cold resistance and other fields.

[0003] Common root pressure measurement methods mainly include bubble pressure meter method, thermal diffusion probe method, wrapped stem flow method and pressure sensor method. However, moso bamboo is a bamboo species with a larger diameter, and its stem is thick, hollow, and its bamboo wall is hard. The diameter at breast height of an adult moso bamboo is between 8 and 16 cm. Therefore, it is of great application value to develop a root pressure measurement method that can measure bamboo with a large diameter. Summary of the invention

[0004] In view of the above problems, the purpose of the present invention is to provide a method and device for measuring the root pressure of bamboo, which can replace sleeves of different diameters according to the size of bamboos and can perform long-term detection of the root pressure of bamboo.

[0005] To achieve the above-mentioned purpose, the present invention proposes the following technical scheme: a method for measuring root pressure of moso bamboo, comprising the following steps: cutting off branches or rods of the moso bamboo to be measured; filling the cut cross section with distilled water; fixing one end of a sleeve on the moso bamboo, and making the cross section located in the sleeve; filling the sleeve with distilled water through the other end of the sleeve, and after the distilled water is filled, connecting the other end of the sleeve with a hose filled with distilled water; the other end of the hose is connected to an electronic pressure sensor through a three-way valve, and reading the root pressure data of the moso bamboo through a data collector connected to the electronic pressure sensor.

[0006] Furthermore, before water is injected into the cross section, the bamboo is trimmed flat with a disposable blade, the squeezed and deformed wood part is removed, and the bamboo shoot sheath is peeled off.

[0007] Furthermore, the sleeve is cup-shaped, the open end of the sleeve is fixed on the bamboo, and a through hole is provided at the center of the other end of the sleeve for installing a conversion head connected to the hose.

[0008] Furthermore, a Parafilm film is wrapped around the outside of the branches or stems of the bamboo shoots, and a thick layer of vaseline is applied, and a sleeve with matching size is put on, so that the Parafilm film and the vaseline seal the sleeve and the bamboo shoots.

[0009] Furthermore, the maximum diameter of the sleeve is 7 cm-8.5 cm, and the minimum diameter of the sleeve is 5.5-7.5 cm.

[0010] Furthermore, the conversion head is a metal conversion head, one end of the metal conversion head connected to the hose is threaded, and the metal conversion head is fixed and sealed by glue.

[0011] Furthermore, vaseline is applied to one end of the metal conversion head connected to the hose.

[0012] Furthermore, the hose is a short silicone tube with a length of 3 to 5 cm.

[0013] The present invention also discloses a bamboo root pressure measuring system, which adopts any of the above-mentioned bamboo root pressure measuring methods, and comprises: a sleeve, a hose, an electronic pressure sensor and a data collector; one end of the sleeve is connected to the bamboo, the caliber of the sleeve matches the diameter of the bamboo, the other end of the sleeve is connected to one end of the hose through a conversion head, and the other end of the hose is connected to the electronic pressure sensor through a three-way valve; the electronic pressure sensor is connected to the data collector through a signal line.

[0014] Furthermore, the sleeve is cup-shaped, the open end of the sleeve is fixed on the bamboo, and a through hole is provided at the center of the other end of the sleeve for installing a conversion head connected to the hose.

[0015] The technical solution of the present invention has at least the following technical effects or advantages: the present invention can replace sleeves of different calibers according to the sizes of bamboos, and can perform root pressure measurement on bamboos of different diameters; the present invention only uses a very short silicone hose at the pressure sensor, avoiding the phenomenon of inaccurate pressure measurement caused by bending of the silicone hose in the field environment. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 is a schematic diagram of a bamboo root pressure measurement system according to an embodiment of the present invention; Figure 2 Schematic diagram of the effect of day and night changes on the root pressure of bamboo in one embodiment of the present invention; Figure 3 Schematic diagram of the diurnal variation of the root pressure of bamboo shoots on the second day in one embodiment of the present invention. DETAILED DESCRIPTION

[0017] In order to enable those skilled in the art to better understand the technical solution of the present invention, the present invention is described in detail through specific embodiments. However, it should be understood that the provision of specific embodiments is only for a better understanding of the present invention, and they should not be understood as limitations of the present invention. In the description of the present invention, it should be understood that the terms used are only for the purpose of description and cannot be understood as indicating or implying relative importance.

[0018] Bamboo plays an important role in reducing soil erosion, land restoration and water conservation. Rapid growth is one of the most notable characteristics of bamboo. As the most representative species, Moso bamboo ( Phyllostachys edulis ) can grow more than 1 meter in 24 hours and more than 20 meters in one and a half months in spring. As a clumping plant, bamboo absorbs and transports water and nutrients through its extensive underground rhizome network. Numerous studies have shown that the rapid growth of bamboo shoots is entirely dependent on a well-developed rhizome network that can expand horizontally extensively and connect young bamboo culms to other mature bamboos. Plants must regulate the supply of water through their roots to meet the water demand of bamboo shoots to maintain water loss and carbon fixation through transpiration. A strong water potential gradient is a key factor in the transport of water from mature bamboo to young bamboo. The rapid growth rate of bamboo and the highly developed rhizome system connecting bamboo culms underground suggest that bamboo may have different hydraulic structures and water use strategies compared to dicotyledonous woody plants. The supply of water is essential to maintain normal physiological functions during the rapid growth period of bamboo. In addition, water potential gradient and root pressure are two important factors for woody bamboo to maintain diurnal water balance and drive rapid growth.

[0019] Unlike trees, bamboo has hollow culms and no secondary growth, which means that the xylem vessels of bamboo must remain functional throughout its life cycle. Bamboo has a strong ability to avoid cavitation due to xylem embolism. Root pressure transported through a highly developed rhizome system may help repair xylem embolism at night and is beneficial to the growth and persistence of bamboo. In addition, root pressure is essential for woody bamboo to maintain diurnal water balance. All of these characteristics and structural features of bamboo may explain the mechanism of its rapid growth. Bamboo shoots, which lack branches and leaves, are an ideal model to study the diurnal changes in root pressure.

[0020] In order to solve the problems existing in the prior art that in the field environment, the silicone hose may bend and other force majeure phenomena, resulting in inaccurate pressure measurement, and the existing method can only be used to detect the root pressure measurement of bamboo species with a small diameter (within 2 cm in diameter), and it is difficult to measure the root pressure of bamboo with a diameter of more than 6 cm, and the existing method cannot replace the sleeve according to the size of the bamboo diameter, and does not have a broad spectrum. The present invention proposes a method and a measuring device for measuring the root pressure of bamboo, which can replace sleeves of different calibers according to the size of the bamboo, and can measure the root pressure of bamboos of different diameters. This method only uses a very short silicone hose at the pressure sensor, avoiding the phenomenon of inaccurate pressure measurement caused by the bending of the silicone hose in the field environment. The scheme of the present invention is described in detail below through embodiments in conjunction with the accompanying drawings.

[0021] Embodiment 1 This embodiment discloses a method for measuring root pressure of moso bamboo, comprising the following steps: S1 cuts off the branches or stems of the bamboo to be tested.

[0022] Cut off the branches or stems of the bamboo to be tested. Before injecting water into the cross section, use a disposable blade to trim the bamboo flat, remove the squeezed and deformed wood, and peel off the bamboo sheath.

[0023] S2 fills the cut cross section with distilled water.

[0024] S3 fixes one end of the sleeve on the bamboo and makes the cross section located in the sleeve.

[0025] The sleeve is cup-shaped, the open end of the sleeve is fixed on the bamboo, and a through hole is set at the center of the other end of the sleeve for installing a conversion head connected to a hose. The maximum diameter of the sleeve is 7cm-8.5cm, and the minimum diameter of the sleeve is 5.5-7.5cm. Wrap a Parafilm film around the outside of the branches or stems of the bamboo shoots, and apply a thick layer of vaseline, and then put on a sleeve of matching size, so that the Parafilm film and vaseline can seal the sleeve and the bamboo.

[0026] In this embodiment, the sleeve can be a cup with a small hole at the bottom. The cup is preferably a cup with a larger bottom diameter and a smaller cup mouth diameter. The cup is turned upside down to serve as the sleeve. For example, it can be a cylindrical Lock&Lock mixing cup, i.e., a Lock&Lock cup. For example, the Lock&Lock cup model HPP931H has a capacity of 470 mL, a minimum diameter of 6.0 cm, and a maximum diameter of 7.3 cm, and is used to measure the root pressure of bamboo with a diameter between 6.0-7.3 cm. The Lock&Lock cup model HPP934H has a capacity of 690 mL, a minimum diameter of 7.3 cm, and a maximum diameter of 8.0 cm, and is used to measure the root pressure of bamboo with a diameter between 7.3-8.0 cm.

[0027] In this embodiment, the conversion head is a metal conversion head, and one end of the metal conversion head connected to the hose is threaded, and the metal conversion head is fixed and sealed by glue. Vaseline is applied to one end of the metal conversion head connected to the hose. The hose is a short tube. The short tube is a silicone hose with an inner diameter of 6.2 mm, an outer diameter of 9 mm, a thickness of 1.4 mm, and a length of 3 to 5 cm.

[0028] S4 fills the sleeve with distilled water through the other end of the sleeve. After the distilled water is filled, the other end of the sleeve is connected to the hose filled with distilled water.

[0029] It is necessary to ensure that there are no bubbles in the cannula and hose and that they are well sealed during the measurement process. If there are bubbles, they must be removed or reconnected in time.

[0030] The other end of the S5 hose is connected to an electronic pressure sensor through a three-way valve, and the root pressure data of the bamboo is read through a data collector connected to the electronic pressure sensor.

[0031] Before connection and use, the electronic pressure sensor needs to be calibrated for correlation coefficient, and the electronic pressure sensor and data collector are connected with a signal line. After the experiment, the data in the data collector is read out to obtain the root pressure data.

[0032] Embodiment 2 Based on the same inventive concept, this embodiment verifies the method in the first embodiment through actual measurement results. In this embodiment, the sleeve is a lock-and-lock cup with a hole in the bottom center, and six different bamboo shoots (about 6 meters high) are selected for dynamic detection of the root pressure dynamics at the base of the bamboo. The experimental measurement was carried out at an experimental site of Jiangxi Academy of Forestry Sciences (28°44′40″ N, 115°48′41″ E, 41 meters above sea level). During the experiment, the selected bamboo shoots were cut horizontally at the base 10 cm above the ground. The cut surface of the bamboo shoot was polished flat with a new razor blade. Since the bamboo is hollow, the bamboo cavity needs to be filled with distilled water. Peel off the bamboo shoot sheath, wrap 2-3 circles of Parafilm™ M film around the outside of the bamboo shoot stem, and apply a thick layer of vaseline. Put on a root pressure detection sleeve with a suitable caliber, and ensure that the Parafilm™ M film and vaseline can seal the detection sleeve and bamboo shoot after compaction. Remove the silicone hose from the metal converter, inject distilled water from the converter until there is no gas in the sleeve, then apply vaseline on the metal converter, and reconnect the silicone hose and the metal converter.

[0033] Measurements of root pressure showed circadian variations, with negative values ​​during the day and positive values ​​at night. The root pressure of bamboo shoots continued to rise between 7:00 pm and 3:30 am and remained positive until 7:30 am. At 7:45 am, two hours after the sun rose, the pressure dropped sharply to negative values, and these values ​​initially showed a downward trend, then gradually returned to zero and finally became positive again at 7:00 pm. The maximum value of root pressure was about 69.9 kPa, which can lift water to a height of 7 meters at 3:00 am. Figure 2 As shown in Figure 2, it is well known that truncation damage is lethal to bamboo shoots, and only half of the shoots could be used for further measurements on the second day. Although the root pressure values ​​on the second day were weaker, they still showed a similar trend to that on the first day, as shown in Figure 2. Figure 3 shown.

[0034] It can be seen that the method in Example 1 can replace sleeves of different calibers according to the size of bamboos, and root pressure measurement can be performed on bamboos of different diameters.

[0035] Embodiment 3 Based on the same inventive concept, this embodiment discloses a bamboo root pressure measurement system, which adopts any of the above-mentioned bamboo root pressure measurement methods, including: a casing, a hose, an electronic pressure sensor and a data collector; One end of the sleeve is connected to the bamboo, the diameter of the sleeve matches the diameter of the bamboo, the other end of the sleeve is connected to one end of the hose through a conversion head, the other end of the hose is connected to the electronic pressure sensor through a three-way valve; the electronic pressure sensor is connected to the data collector through a signal line. The sleeve is cup-shaped, the open end of the sleeve is fixed on the bamboo, and a through hole is set at the center of the other end of the sleeve for installing the conversion head connected to the hose.

[0036] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention rather than to limit them. Although the present invention has been described in detail with reference to the above embodiments, ordinary technicians in the relevant field should understand that the specific implementation of the present invention can still be modified or replaced by equivalents, and any modification or equivalent replacement that does not deviate from the spirit and scope of the present invention should be included in the protection scope of the claims of the present invention. The above content is only a specific implementation of the present invention, but the protection scope of the present invention is not limited thereto. Any technician familiar with the technical field can easily think of changes or replacements within the technical scope disclosed by the present invention, which should be included in the protection scope of the present invention. Therefore, the protection scope of the present invention shall be based on the protection scope of the claims.

Claims

1. A method for measuring root pressure of bamboo, characterized in that: The following steps are involved: Cut off the branches or stems of the bamboo to be tested; Fill the cut cross section with distilled water; Fixing one end of the sleeve on the bamboo, and making the cross section located in the sleeve; Fill the sleeve with distilled water through the other end of the sleeve, and after the distilled water is filled, connect the other end of the sleeve to a hose filled with distilled water; The other end of the hose is connected to an electronic pressure sensor through a three-way valve, and the root pressure data of the bamboo is read through a data collector connected to the electronic pressure sensor.

2. The method for measuring root pressure of bamboo according to claim 1, wherein: Before water is injected into the cross section, the bamboo is trimmed flat with a disposable blade, the squeezed and deformed wood part is removed, and the bamboo shoot sheath is peeled off.

3. The method for measuring root pressure of bamboo according to claim 1, wherein: The sleeve is cup-shaped, the open end of the sleeve is fixed on the bamboo, and a through hole is arranged at the center of the other end of the sleeve for installing a conversion head connected with a hose.

4. The method for measuring root pressure of bamboo according to claim 3, wherein: Wrap the Parafilm film around the outside of the branches or stems of the bamboo shoots, apply a thick layer of vaseline, and put on a sleeve with matching size, so that the Parafilm film and vaseline can seal the sleeve and the bamboo shoots.

5. The method for measuring root pressure of bamboo according to claim 3, wherein: The maximum diameter of the sleeve is 7cm-8.5cm, and the minimum diameter of the sleeve is 5.5-7.5cm.

6. The method for measuring root pressure of bamboo according to claim 1, characterized in that: The conversion head is a metal conversion head, one end of the metal conversion head connected to the hose is threaded, and the metal conversion head is fixed and sealed by glue.

7. The method for measuring root pressure of bamboo according to claim 6, characterized in that: Vaseline is applied to one end of the metal conversion head connected to the hose.

8. The method for measuring root pressure of bamboo according to claim 1, wherein: The hose is a short silicone tube with a length of 3 to 5 cm.

9. A bamboo root pressure measurement system, characterized in that: The method for measuring root pressure of bamboo as claimed in any one of claims 1 to 8 comprises: a casing, a hose, an electronic pressure sensor and a data collector; One end of the sleeve is connected to the bamboo, the caliber of the sleeve matches the diameter of the bamboo, the other end of the sleeve is connected to one end of a hose through a conversion head, and the other end of the hose is connected to an electronic pressure sensor through a three-way valve; the electronic pressure sensor is connected to a data collector through a signal line.

10. The bamboo root pressure measuring system according to claim 9, characterized in that: The sleeve is cup-shaped, the open end of the sleeve is fixed on the bamboo, and a through hole is arranged at the center of the other end of the sleeve for installing a conversion head connected with a hose.

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