Tea polyphenol / lauric acid / gelatin natural drug-loaded wound dressing and preparation method thereof

Abstract

The invention relates to the field of wound dressings, in particular to a tea polyphenol / lauric acid / gelatin natural drug-loaded wound dressing and a preparation method thereof. The wound dressing is prepared from natural materials including tea polyphenol, lauric acid and gelatin, and the tea polyphenol is used as a bacteriostatic drug and can achieve a good bacteriostatic effect. According to the invention, the adopted lauric acid can realize reversible solid-liquid conversion at 44 DEG C, so that the release of the loaded tea polyphenol under temperature control is realized. The gelatin fibers in oriented arrangement have better mechanical properties and can promote cell migration. The prepared wound dressing has good effects of promoting wound healing and inhibiting bacteria. The tea polyphenol / lauric acid / gelatin natural drug-loaded wound dressing is simple to prepare and has a wide application prospect in the field of wound dressings.

Description

Technical Field

[0001] The invention belongs to the technical field of wound dressings, and in particular relates to a tea polyphenol / lauric acid / gelatin natural drug-carrying wound dressing and a preparation method thereof. Background Art

[0002] With the continuous development of wound dressing technology, the traditional auxiliary material - gauze, due to its high permeability, will cause the wound surface to dehydrate, and easy to adhere to the wound surface and cause secondary damage to the wound, etc., has been gradually eliminated. More and more researchers have begun to pay attention to wound dressings such as nanofibers. Nanofibers have high air permeability and can flexibly load various antibacterial substances in the fiber membrane, giving the fiber membrane different antibacterial activities. However, the current research direction of wound dressings is mainly to achieve wound treatment by adding a single antibacterial drug, but this will cause the production of drug-resistant bacteria, thereby inducing more serious diseases.

[0003] Tea polyphenols are a natural small molecule antibacterial substance with good water solubility and high bioavailability in wounds. Lauric acid is a natural substance that can control solid-liquid transition by temperature, and the transition temperature is about 44°C, which will not cause damage to the wound. Therefore, temperature-controlled tea polyphenol release can be achieved by blending tea polyphenols and lauric acid into nanoparticles.

[0004] Gelatin nanofibers have good biocompatibility, good drug carriers, biodegradability and good physiological properties. However, gelatin nanofibers have poor mechanical properties, which inhibits their use as wound dressings. Fiber orientation can effectively improve the mechanical properties of gelatin nanofibers. And oriented nanofibers are more capable of cell migration than randomly arranged nanofibers, which is more conducive to wound healing. Summary of the invention

[0005] In view of this, the present invention provides a tea polyphenol / lauric acid / gelatin natural drug-carrying wound dressing and a preparation method thereof. The film wound dressing has high antibacterial activity.

[0006] The invention provides a tea polyphenol / lauric acid / gelatin natural drug-carrying wound dressing. The wound dressing is prepared using three natural materials, namely, tea polyphenol, lauric acid and gelatin, as raw materials.

[0007] The specific technical solutions of the present invention are as follows:

[0008] Step 1: Dissolve gelatin in a mixed solution of acetic acid, ethyl acetate and distilled water, and stir on a magnetic stirrer at room temperature;

[0009] Step 2: The solution obtained in step 1 is subjected to a parallel electrode method to prepare oriented gelatin fibers;

[0010] Step 3: Dissolve tea polyphenols and lauric acid in a mixed solution of dichloromethane and ethanol, and stir on a magnetic stirrer at room temperature;

[0011] Step 4: spraying the solution obtained in step 3 onto the curved nanofibers prepared in step 2 by electrostatic spraying, and finally preparing a tea polyphenols / lauric acid / gelatin natural drug-loaded wound dressing;

[0012] Preferably, the final mass concentration of the gelatin solution is 10%.

[0013] In the present invention, the preparation method of the gelatin solution is as follows: gelatin is mixed with acetic acid, ethyl acetate and distilled water (volume ratio is 4:3:2) at room temperature to obtain a gelatin solution; the time is 6 to 12 hours;

[0014] The mixing is preferably carried out under stirring conditions, more preferably under magnetic stirring conditions; the stirring rate is 300 to 400 rpm;

[0015] Preferably, the final mass concentration of the tea polyphenols / lauric acid solution is 0.1% tea polyphenols and 20% lauric acid.

[0016] In the present invention, the preparation method of the mixed solution of tea polyphenols and lauric acid is as follows: tea polyphenols and lauric acid are mixed with dichloromethane and ethanol (volume ratio is 4:1) at room temperature to obtain a tea polyphenols / lauric acid solution;

[0017] The mixing is preferably carried out under stirring conditions, more preferably under magnetic stirring conditions; the stirring rate is 300 to 400 rpm; the stirring time is 1 to 2 hours;

[0018] In the present invention, the preparation method of the oriented gelatin fiber described in step 2 is: take two iron sheets of the same length and size, tilt them at a certain angle to contact the collector, control the distance between the two iron sheets to be 2-3 cm, and the spinning voltage to be 14 kV-15 kV; the flow rate is 0.3 mL / h, and the oriented gelatin fiber is obtained;

[0019] In the present invention, the preparation method of the tea polyphenol / lauric acid / gelatin natural drug-carrying wound dressing described in step 4 is: fixing the oriented gelatin fibers to a drum collector, the spinning voltage is 20 kV to 21 kV; the flow rate is 3 mL / h, and finally obtaining the tea polyphenol / lauric acid / gelatin natural drug-carrying wound dressing;

[0020] The innovation of the present invention is as follows: the present invention uses all-natural materials to prepare wound dressings, and the oriented nanofibers can improve their mechanical properties, which is more conducive to cell migration and thus promotes wound healing. The wound dressing has good biocompatibility and wound healing effect. Since tea polyphenols and gelatin are both water-soluble substances, the combination of the two will cause the tea polyphenols to be released quickly, so lauric acid, which can control the solid-liquid transition by temperature, is added to achieve temperature-controlled release of tea polyphenols. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the technical solutions in the embodiments of the present invention will be clearly and completely described below.

[0022] Figure 1 This is a summary diagram of the present invention; Figure 2 This is an electron microscope image of the oriented gelatin fiber of Example 1 of the present invention; Figure 3 This is the orientation distribution diagram of the oriented gelatin fiber of Example 1; DETAILED DESCRIPTION

[0023] The present invention provides a polyphenol / lauric acid / gelatin natural drug-loaded wound dressing and a preparation method thereof. The invention prepares gelatin nanofibers with an oriented structure by a parallel electrode method, and then a mixture of tea polyphenols and lauric acid is loaded on the oriented gelatin fibers by an electrostatic spraying method. The lauric acid used can be temperature-controlled for solid-liquid conversion, and can release tea polyphenols under temperature control, thereby solving the problem of wounds being infected by bacteria and deteriorating into drug-resistant bacteria during the wound healing process.

[0024] The present invention is further described in detail below by examples:

[0025] Example 1: Preparation of oriented gelatin fiber membrane

[0026] (1) Preparation of gelatin solution: 0.4010 g of gelatin was added to a mixed solution containing 1 mL of acetic acid, 750 μL of ethyl acetate, and 500 μL of purified water. The mixture was mixed at room temperature and stirred under magnetic stirring at a speed of 300 to 400 rpm for 6 to 12 h. The final gelatin solution concentration was 10% (w / v).

[0027] (2) Preparation of oriented gelatin fibers: Take two iron sheets of the same length and size, tilt them at a certain angle to contact the collector, control the distance between the two iron sheets to be 2 to 3 cm, and the spinning voltage to be 14 kV to 15 kV; the flow rate is 0.3 mL / h to obtain oriented gelatin fibers.

Claims

1. A natural drug-loaded wound dressing, characterized in that: The wound dressing is gelatin, the drug is tea polyphenols, and lauric acid is added as a drug temperature-controlled release material; The nanofiber dressing is prepared by electrostatic spinning and electrostatic spraying.

2. The method for preparing the wound dressing according to claim 1, characterized in that: The following steps are involved: Step 1: Dissolve gelatin in a mixed solution of acetic acid, ethyl acetate and distilled water, and stir on a magnetic stirrer at room temperature; Step 2: Prepare oriented gelatin fibers by using the solution obtained in step 1 through a parallel electrode method; Step 3: Dissolve tea polyphenols and lauric acid in a mixed solution of dichloromethane and ethanol, and stir on a magnetic stirrer at room temperature; Step 4: spray the solution obtained in step 3 onto the nanofibers prepared in step 2 by electrostatic spraying, and finally prepare a tea polyphenols / lauric acid / gelatin natural drug-loaded wound dressing.

3. The preparation method according to claim 2, characterized in that: The mass concentration of the gelatin solution is 10%, the ratio of acetic acid, ethyl acetate and distilled water is 4:3:2, the spinning voltage is 14kV-15kV, and the flow rate is 0.3mL / h.

4. The preparation method according to claim 2, characterized in that: The final mass concentration of the tea polyphenols is 0.1%, the final mass concentration of lauric acid is 20%, the ratio of dichloromethane to ethanol is 4:1, the spinning voltage is 20 kV-21 kV, and the flow rate is 3 mL / h.

5. The natural drug-carrying wound dressing as claimed in claim 1, which is used in wound healing.

Images