A preparation device and preparation method for nicotinamide riboside by applying the biological enzyme method

Abstract

The present invention relates to the technical field of ribose preparation, specifically a preparation device and a preparation method for preparing nicotinamide riboside by using a biological enzyme method, including a reaction kettle, on which an adjustment mechanism is arranged, and a pH balance mechanism is also arranged; the reaction kettle includes a motor, on which a rotating rod is arranged, on the rotating rod there are stirring paddles, and an outlet plate is also arranged on the reaction kettle, and a handle is arranged on the outlet plate; the adjustment mechanism includes a first piston rod, on which a second piston rod is arranged, on the second piston rod there is a first insertion rod, and the first piston rod penetrates through the second piston rod. The purpose of the present invention is to provide the insertion of the first insertion rod with the first piston rod and the second piston rod, control whether the first insertion rod is connected to the first piston rod, and respectively control the amount of raw materials added and the amount of catalyst added as needed, so that the reaction proceeds maximally and the product nicotinamide riboside can be obtained to the greatest extent.

Description

Technical Field

[0001] The present invention relates to the technical field of ribose preparation, and specifically to a preparation device and a preparation method for nicotinamide ribose by applying a bio-enzymatic method. Background Art

[0002] Nicotinamide ribose, abbreviated as NR, is a compound with various biological activities. It is a derivative of vitamin B3 and an important precursor of nicotinamide adenine dinucleotide. The preparation of nicotinamide ribose by a bio-enzymatic method is a green and efficient synthesis method. It uses an enzyme as a catalyst and reacts under mild conditions, avoiding some disadvantages in chemical synthesis methods, such as harsh reaction conditions, difficult separation and purification of products, and environmental pollution.

[0003] During the existing process of preparing nicotinamide ribose by a bio-enzymatic method, it is difficult to adjust the amounts of the reaction products and the catalyst, resulting in low reaction efficiency. Moreover, manual feeding is prone to errors, causing waste of catalyst resources. Summary of the Invention

[0004] The purpose of the present invention is to provide the insertion of the first insertion rod, the first piston rod, and the second piston rod, control whether the first insertion rod is connected to the first piston rod, and respectively control the amounts of the raw materials and the catalyst added as needed, so as to maximize the reaction and obtain the product nicotinamide ribose to the greatest extent.

[0005] To achieve the above purpose, the present invention provides the following technical solution: A preparation device and a preparation method for nicotinamide ribose by applying a bio-enzymatic method, including a reaction kettle, a regulating mechanism is arranged on the reaction kettle, and a pH balancing mechanism is also arranged on the reaction kettle;

[0006] The reaction kettle includes a motor, a rotating rod is arranged on the motor, a stirring paddle is arranged on the rotating rod, an outlet plate is also arranged on the reaction kettle, and a handle is arranged on the outlet plate;

[0007] The regulating mechanism includes a first piston rod, a second piston rod is arranged on the first piston rod, a first insertion rod and a third piston rod are respectively arranged at both ends of the second piston rod, a first insertion rod and a second insertion rod are respectively arranged on the second piston rod and the third piston rod, a first piston pipeline is arranged on the second piston rod, a first piston block is also arranged at one end of the second piston rod, a second piston block is also arranged on the first piston rod passing through the first piston block, a first feed barrel is arranged on the first piston pipeline above the first piston block, a second feed barrel is arranged on the first piston pipeline above the second piston block, and a feed pipe is also arranged on the first piston pipeline;

[0008] The pH balance mechanism includes a storage barrel, and a water inlet pipe is further provided on the storage barrel. A second piston pipe is provided on the water inlet pipe, and a fourth piston rod is provided on the second piston pipe. One end of the fourth piston rod is fixedly connected to the third piston rod. An inlet water barrel is further provided on the water inlet pipe, and a limiting pipe is provided on the storage barrel. A limiting ball is provided on the limiting pipe.

[0009] Preferably, the first piston pipe and the feed pipe are designed with a hollow interior, and their dimensions are adapted to the second piston block and the first piston block respectively.

[0010] Preferably, holes are provided at one ends of the second piston rod and the first piston rod close to the first piston block, and their dimensions are adapted to the first insertion rod.

[0011] Preferably, holes are provided at one ends of the third piston rod and the first piston rod close to the second piston block, and their dimensions are adapted to the second insertion rod.

[0012] Preferably, the second piston pipe is designed with a hollow interior, and its dimension is adapted to the fourth piston rod.

[0013] Preferably, a plurality of holes are provided on the reaction kettle, and their dimensions are respectively adapted to the discharge plate and the handle.

[0014] Preferably, a scraping plate is provided on the stirring paddle, and the scraping plate is attached to the inner wall of the reaction kettle.

[0015] Preferably, the limiting pipe is designed with a hollow interior, and grooves of different sizes are provided inside the limiting pipe. The limiting ball is restricted in the groove by the limiting pipe.

[0016] Preferably, a support column is provided on the storage barrel, and both the storage barrel and the water inlet pipe are designed with a hollow interior.

[0017] A preparation method for preparing nicotinamide riboside by biocatalysis includes the following steps:

[0018] Step 1: Put the mixture of nicotinamide and purine nucleoside as raw materials into the first feed barrel, control the amount of raw materials entering the reaction kettle by adjusting the second piston rod, and then put the enzyme solution as the catalyst for the reaction into the second feed barrel. Control the amount of catalyst entering the reaction kettle by adjusting the first piston rod. Control whether the first insertion rod is connected to the first piston rod and the second piston rod through the insertion of the first insertion rod.

[0019] Step 2: After the reactants and the catalyst are added to the reaction kettle, adjust the connection between the fourth piston rod and the first piston rod by adjusting the insertion of the second insertion rod with the third piston rod and the first piston rod. Adjust the amount of the alkaline solution added to the inlet water barrel by adjusting the first piston rod to adjust the fourth piston rod, and the alkaline solution enters the water inlet pipe and then enters the storage barrel.

[0020] Step 3: Start the motor to drive the rotating rod, which drives the stirring paddle to rotate, stirring the mixed liquid in the reaction kettle. At the same time, the stirring paddle squeezes the limiting balls on the inner wall of the reaction kettle, causing the alkaline solution in the storage bucket to enter the interior of the reaction kettle through the limiting pipe, and the water generated by the neutralization reaction adjusts the pH value of the reaction environment.

[0021] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0022] 1. Through the insertion of the first insertion rod with the first piston rod and the second piston rod, the present invention controls whether the first insertion rod is connected to the first piston rod. According to needs, the amounts of the added raw materials and the added catalyst can be controlled separately, enabling the reaction to proceed maximally and obtaining the product nicotinamide ribose to the greatest extent.

[0023] 2. After the reaction starts, the present invention adjusts the connection between the fourth piston rod and the first piston rod by adjusting the insertion of the second insertion rod with the third piston rod and the first piston rod. By adjusting the first piston rod, the amount of the alkaline solution added to the water inlet bucket is adjusted, which enters the water inlet pipe and then into the storage bucket. This facilitates the entry of the alkaline solution from the storage bucket into the reaction kettle during the reaction, preventing the manual adjustment of the pH value during the reaction from affecting the reaction, automatically adjusting the pH balance to ensure the normal progress of the reaction, and efficiently producing nicotinamide ribose.

[0024] 3. The present invention simultaneously starts the motor to drive the rotating rod, which drives the stirring paddle to rotate, stirring the mixed liquid in the reaction kettle. At the same time, the stirring paddle squeezes the limiting balls on the inner wall of the reaction kettle, causing the alkaline solution in the storage bucket to enter the interior of the reaction kettle through the limiting pipe, neutralizing the water generated by the reaction, and adjusting the pH value of the reaction environment. This prevents the water generated by the reaction from changing the pH of the reaction environment and affecting the activity of the enzyme, thereby affecting the reaction efficiency and ensuring the efficient progress of the reaction. Description of the Drawings

[0025] Figure 1 is the overall structural schematic diagram of the present invention;

[0026] Figure 2 is the first partial structural schematic diagram of the present invention;

[0027] Figure 3 is of the present invention Figure 2 magnified view of part A;

[0028] Figure 4 is of the present invention Figure 2 magnified view of part B;

[0029] Figure 5 is the second partial structural schematic diagram of the present invention;

[0030] Figure 6 is the third partial structural schematic diagram of the present invention;

[0031] Figure 7 For the present invention Figure 6 Enlarged view of part C in

[0032] In the figure: 1, reaction kettle; 2, adjusting mechanism; 3, ph balancing mechanism; 11, handle; 12, rotating rod; 13, stirring paddle; 14, scraper; 15, discharge plate; 16, motor; 21, first piston rod; 22, first piston pipe; 23, first piston block; 24, second piston block; 25, first feed bucket; 26, second feed bucket; 27, first insertion rod; 271, second piston rod; 28, second insertion rod; 281, third piston rod; 29, feed pipe; 31, storage bucket; 32, support column; 33, water inlet pipe; 34, water inlet bucket; 35, second piston pipe; 36, fourth piston rod; 37, limiting pipe; 38, limiting ball. Specific embodiments

[0033] To make the objectives, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. Therefore, the following detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed present invention, but merely represents selected embodiments of the present invention. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the scope of protection of the present invention.

[0034] The present invention provides a preparation device and a preparation method for preparing nicotinamide riboside by using a biological enzyme method, including a reaction kettle 1, an adjusting mechanism 2 is arranged on the reaction kettle 1, and a ph balancing mechanism 3 is also arranged on the reaction kettle 1;

[0035] The reaction kettle 1 includes a motor 16, a rotating rod 12 is arranged on the motor 16, a stirring paddle 13 is arranged on the rotating rod 12, a discharge plate 15 is also arranged on the reaction kettle 1, and a handle 11 is arranged on the discharge plate 15;

[0036] The adjusting mechanism 2 includes a first piston rod 21. At both ends of the first piston rod 21, a second piston rod 271 and a third piston rod 281 are respectively arranged. On the second piston rod 271 and the third piston rod 281, a first insertion rod 27 and a second insertion rod 28 are respectively arranged. The first piston rod 21 penetrates through the second piston rod 271. On the second piston rod 271, a first piston pipe 22 is arranged. At one end of the second piston rod 271, a first piston block 23 is further arranged. The first piston rod 21 penetrates through the first piston block 23 and a second piston block 24 is further arranged. On the first piston pipe 22, above the first piston block 23, a first feed bucket 25 is arranged. On the first piston pipe 22, above the second piston block 24, a second feed bucket 26 is arranged. On the first piston pipe 22, a feed pipe 29 is further arranged.

[0037] The pH balancing mechanism 3 includes a storage bucket 31. On the storage bucket 31, a water inlet pipe 33 is further arranged. On the water inlet pipe 33, a second piston pipe 35 is arranged. On the second piston pipe 35, a fourth piston rod 36 is arranged. One end of the fourth piston rod 36 is fixedly connected to the third piston rod 281. On the water inlet pipe 33, a water inlet bucket 34 is further arranged. On the storage bucket 31, a restricting pipe 37 is further arranged. On the restricting pipe 37, a restricting ball 38 is arranged.

[0038] Before the reaction starts, the mixed solution of nicotinamide and purine nucleoside is put into the first feed bucket 25 as a raw material. The amount of the raw material entering the reaction kettle 1 is controlled by adjusting the second piston rod 271. Then, an enzyme solution is put into the second feed bucket 26 as a catalyst for the reaction. The amount of the catalyst entering the reaction kettle 1 is controlled by adjusting the first piston rod 21. By inserting the first insertion rod 27 with the first piston rod 21 and the second piston rod 271, it is controlled whether the first insertion rod 27 is connected to the first piston rod 21. According to needs, the amount of the added raw material and the amount of the added catalyst can be respectively controlled, so that the reaction proceeds maximally, and the product nicotinamide ribose can be obtained to the maximum extent.

[0039] At the same time, since water will be generated during the process of generating nicotinamide ribose, which causes the pH of the environment to change and affects the activity of the enzyme, after the reaction starts, by adjusting the insertion of the second insertion rod 28 with the third piston rod 281 and the first piston rod 21, the connection between the fourth piston rod 36 and the first piston rod 21 is adjusted. By adjusting the first piston rod 21 to adjust the fourth piston rod 36, the amount of the alkaline solution added to the water inlet bucket 34 is adjusted. It enters the water inlet pipe 33 and then enters the storage bucket 31, which is convenient to enter the reaction kettle 1 from the storage bucket 31 during the reaction, preventing the manual adjustment of the pH value during the reaction from affecting the reaction, automatically adjusting the pH balance to ensure the normal progress of the reaction, and efficiently producing nicotinamide ribose.

[0040] Meanwhile, the motor 16 is turned on to drive the rotating rod 12, which drives the stirring paddle 13 to rotate, stirring the mixed liquid in the reaction kettle 1. At the same time, the stirring paddle 13 squeezes the limiting balls 38 on the inner wall of the reaction kettle 1, causing the alkaline solution in the storage barrel 31 to enter the interior of the reaction kettle 1 through the limiting pipe 37, neutralizing the water generated by the reaction, adjusting the pH value of the reaction environment, preventing the water generated by the reaction from changing the pH of the reaction environment and affecting the activity of the enzyme, and affecting the reaction efficiency, ensuring the efficient progress of the reaction.

[0041] In an alternative embodiment, the first piston pipe 22 and the feed pipe 29 are designed to be hollow, and their sizes are adapted to the second piston block 24 and the first piston block 23 respectively.

[0042] It should be noted that the first piston pipe 22 is designed to be hollow, facilitating the movement of the first piston block 23 and the second piston block 24 in the first piston pipe 22 to adjust the feed rate.

[0043] In an alternative embodiment, holes are provided at one end of the second piston rod 271 and the first piston rod 21 close to the first piston block 23, and their sizes are adapted to the first insertion rod 27.

[0044] It should be noted that when the first insertion rod 27 disengages from the device, moving the first piston rod 21 does not drive the second piston rod 271 to move, only the second piston block 24 is adjusted. When the first insertion rod 27 is inserted into the device, the first piston rod 21 and the second piston rod 271 are fixed, and moving the first piston rod 21 can drive the second piston rod 271 to move, adjusting the first piston block 23 and the second piston block 24 simultaneously, achieving the purpose of adjusting the feed either simultaneously or separately.

[0045] In an alternative embodiment, holes are provided at one end of the third piston rod 281 and the first piston rod 21 close to the second piston block 24, and their sizes are adapted to the second insertion rod 28.

[0046] It should be noted that when the second insertion rod 28 disengages from the device, moving the first piston rod 21 does not drive the fourth piston rod 36 to move. When the second insertion rod 28 is inserted into the first piston rod 21, the third piston rod 281 and the first piston rod 21 are fixed, and moving the first piston rod 21 drives the third piston rod 281 to move, driving the fourth piston rod 36 to move, adjusting the water inflow volume in the water inlet bucket 34, achieving the purpose of adjusting the feed and water inflow respectively and improving the practicality of the device.

[0047] In an alternative embodiment, the third piston rod 281 is fixedly connected to the fourth piston rod 36, and the second piston pipe 35 is designed to be hollow, with its size adapted to the fourth piston rod 36.

[0048] It should be noted that the movement of the third piston rod 281 drives the fourth piston rod 36 to move in the second piston pipe 35, thereby adjusting the feeding speed of the alkaline solution in the water inlet bucket 34.

[0049] In an alternative embodiment, the reaction kettle 1 is provided with a plurality of holes, the sizes of which are respectively adapted to the discharge plate 15 and the handle 11.

[0050] It should be noted that starting the motor 16 drives the rotating rod 12 to rotate, which in turn drives the stirring paddle 13 to rotate, stirring the reaction. At the same time, the rotation of the stirring paddle 13 drives the scraper 14 to scrape the solution on the inner wall of the reaction kettle 1, making the reaction more complete.

[0051] In an alternative embodiment, the stirring paddle 13 is provided with a scraper 14, and the scraper 14 is in contact with the inner wall of the reaction kettle 1.

[0052] It should be noted that after the reaction is completed, the handle 11 is rotated to drive the discharge plate 15 to rotate, so as to take out the reacted solution from the reaction kettle 1, prevent the solution from remaining in the device, and at the same time facilitate the separation of the generated products.

[0053] In an alternative embodiment, the restricting tube 37 has a hollow design, and different-sized grooves are provided inside the restricting tube 37. The restricting ball 38 is restricted in the grooves by the restricting tube 37.

[0054] It should be noted that when the stirring paddle 13 rotates, it squeezes the restricting ball 38 and pushes the restricting ball 38 a certain distance, so that the alkaline solution enters the reaction kettle 1 from the storage bucket 31 through the restricting tube 37, maintaining the pH balance of the reaction in the reaction kettle 1 and ensuring the normal and efficient progress of the reaction.

[0055] In an alternative embodiment, the storage bucket 31 is provided with support columns 32, and both the storage bucket 31 and the water inlet pipe 33 have a hollow design.

[0056] It should be noted that the support columns 32 fixedly support the storage bucket 31.

[0057] A preparation method for preparing nicotinamide riboside by a biocatalytic method comprises the following steps:

[0058] Step 1: Put the mixture of nicotinamide and purine nucleoside as raw materials into the first feeding bucket 25, control the amount of raw materials entering the reaction kettle 1 by adjusting the second piston rod 271, then put the enzyme solution as the catalyst for the reaction into the second feeding bucket 26, control the amount of catalyst entering the reaction kettle 1 by adjusting the first piston rod 21, and control whether the first plugging rod 27 is connected to the first piston rod 21 through the plugging of the first plugging rod 27 with the first piston rod 21 and the second piston rod 271;

[0059] Step 2: After the reactants and the catalyst are added to the reaction kettle 1, the connection between the fourth piston rod 36 and the first piston rod 21 is adjusted by adjusting the insertion of the second insertion rod 28 with the third piston rod 281 and the first piston rod 21. The amount of the alkaline solution added to the water inlet bucket 34 is adjusted by adjusting the first piston rod 21 to adjust the fourth piston rod 36, and then it enters the water inlet pipe 33 and then into the storage bucket 31;

[0060] Step 3: The motor 16 is started to drive the rotating rod 12, which drives the stirring paddle 13 to rotate, and the mixed liquid in the reaction kettle 1 is stirred. At the same time, the stirring paddle 13 squeezes the limiting ball 38 on the inner wall of the reaction kettle 1, so that the alkaline solution in the storage bucket 31 enters the interior of the reaction kettle 1 through the limiting pipe 37, and the water generated by the neutralization reaction adjusts the pH value of the reaction environment.

[0061] Working principle: Before the reaction starts, the mixed solution of nicotinamide and purine nucleoside is put into the first feed bucket 25 as the raw material. The amount of the raw material entering the reaction kettle 1 is controlled by adjusting the second piston rod 271. Then, the enzyme solution is put into the second feed bucket 26 as the catalyst for the reaction. The amount of the catalyst entering the reaction kettle 1 is controlled by adjusting the first piston rod 21. By inserting the first insertion rod 27 with the first piston rod 21 and the second piston rod 271, it is controlled whether the first insertion rod 27 is connected to the first piston rod 21. According to the need, the amount of the added raw material and the amount of the added catalyst can be controlled separately, so that the reaction proceeds maximally and the product nicotinamide ribose can be obtained to the greatest extent.

[0062] At the same time, since water is generated during the process of generating nicotinamide ribose, which causes the change of the pH of the environment and affects the activity of the enzyme, after the reaction starts, the connection between the fourth piston rod 36 and the first piston rod 21 is adjusted by adjusting the insertion of the second insertion rod 28 with the third piston rod 281 and the first piston rod 21. The amount of the alkaline solution added to the water inlet bucket 34 is adjusted by adjusting the first piston rod 21 to adjust the fourth piston rod 36, and then it enters the water inlet pipe 33 and then into the storage bucket 31, which is convenient for entering the reaction kettle 1 from the storage bucket 31 during the reaction, preventing the manual adjustment of the pH value during the reaction from affecting the reaction, automatically adjusting the pH balance to ensure the normal progress of the reaction, and efficiently producing nicotinamide ribose.

[0063] At the same time, the motor 16 is started to drive the rotating rod 12, which drives the stirring paddle 13 to rotate, and the mixed liquid in the reaction kettle 1 is stirred. At the same time, the stirring paddle 13 squeezes the limiting ball 38 on the inner wall of the reaction kettle 1, so that the alkaline solution in the storage bucket 31 enters the interior of the reaction kettle 1 through the limiting pipe 37, neutralizes the water generated by the reaction, adjusts the pH value of the reaction environment, prevents the water generated by the reaction from causing the change of the pH of the reaction environment and affecting the activity of the enzyme, and affects the efficiency of the reaction, and ensures the efficient progress of the reaction.

[0064] The first piston pipe 22 is hollow designed to facilitate the movement of the first piston block 23 and the second piston block 24 in the first piston pipe 22 for adjusting the feeding amount. When the first insertion rod 27 disengages from the device, moving the first piston rod 21 does not drive the second piston rod 271 to move, and only the second piston block 24 is adjusted. When the first insertion rod 27 is inserted into the device, the first piston rod 21 and the second piston rod 271 are fixed, and moving the first piston rod 21 can drive the second piston rod 271 to move, simultaneously adjusting the first piston block 23 and the second piston block 24 to achieve the purpose of adjusting the feeding either simultaneously or separately. When the second insertion rod 28 disengages from the device, moving the first piston rod 21 does not drive the fourth piston rod 36 to move. When the second insertion rod 28 is inserted into the first piston rod 21, the third piston rod 281 and the first piston rod 21 are fixed, and moving the first piston rod 21 drives the third piston rod 281 to move, driving the fourth piston rod 36 to move to adjust the water inflow amount in the water inlet bucket 34, achieving the adjustment of the feeding and the water inflow respectively, improving the practicability of the device. After the reaction is completed, the discharge plate 15 is rotated by turning the handle 11 to take out the reacted solution from the reaction kettle 1, preventing the solution from remaining in the device, and at the same time facilitating the separation of the generated products. When the stirring paddle 13 rotates, it squeezes the limiting ball 38 and pushes the limiting ball 38 a certain distance, enabling the alkaline solution to enter the reaction kettle 1 from the storage bucket 31 through the limiting pipe 37 to maintain the pH balance of the reaction in the reaction kettle 1 and ensure the normal and efficient progress of the reaction.

[0065] Although the embodiments of the present invention have been shown and described, it will be understood by those of ordinary skill in the art that various changes, modifications, substitutions and variations can be made in these embodiments without departing from the principles and spirit of the present invention, and the scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A preparation device for preparing nicotinamide riboside by applying a biological enzyme method, comprising a reaction kettle (1), characterized in that, A regulating mechanism (2) is provided on the reactor (1), and a pH balancing mechanism (3) is also provided on the reactor (1); the reactor (1) includes a motor (16), a rotating rod (12) is provided on the motor (16), a stirring paddle (13) is provided on the rotating rod (12), a discharge plate (15) is also provided on the reactor (1), and a handle (11) is provided on the discharge plate (15). The regulating mechanism (2) includes a first piston rod (21), a second piston rod (271) and a third piston rod (281) are respectively provided at both ends of the first piston rod (21), a first insertion rod (27) and a second insertion rod (28) are respectively provided on the second piston rod (271) and the third piston rod (281), the first piston rod (21) penetrates through the second piston rod (271), a first piston pipe (22) is provided on the second piston rod (271), a first piston block (23) is further provided at one end of the second piston rod (271), a second piston block (24) is further provided that the first piston rod (21) penetrates through the first piston block (23), a first feed bucket (25) is provided on the first piston pipe (22) above the first piston block (23), a second feed bucket (26) is provided on the first piston pipe (22) above the second piston block (24), and a feed pipe (29) is further provided on the first piston pipe (22); the pH balancing mechanism (3) includes a storage bucket (31), a water inlet pipe (33) is further provided on the storage bucket (31), a second piston pipe (35) is provided on the water inlet pipe (33), a fourth piston rod (36) is provided on the second piston pipe (35), one end of the fourth piston rod (36) is fixedly connected to the third piston rod (281), a water inlet bucket (34) is further provided on the water inlet pipe (33), a limiting pipe (37) is further provided on the storage bucket (31), a limiting ball (38) is provided on the limiting pipe (37), the limiting pipe (37) is designed to be hollow, grooves of different sizes are provided inside the limiting pipe (37), and the limiting ball (38) is restricted in the groove by the limiting pipe (37).

2. The preparation device for nicotinamide riboside prepared by applying a bio-enzymatic method according to claim 1, characterized in that, The first piston pipe (22) and the feed pipe (29) are designed to be hollow, and the sizes are adapted to the second piston block (24) and the first piston block (23).

3. The preparation device for nicotinamide riboside prepared by applying a biological enzyme method according to claim 1, characterized in that, Holes are provided at one ends of the second piston rod (271) and the first piston rod (21) close to the first piston block (23), and the sizes are adapted to the first insertion rod (27).

4. A preparation device for preparing nicotinamide riboside by applying a bio-enzymatic method according to claim 1, wherein, Holes are provided at one ends of the third piston rod (281) and the first piston rod (21) close to the second piston block (24), and the sizes are adapted to the second insertion rod (28).

5. The preparation device for nicotinamide riboside prepared by a biological enzyme method according to claim 1, characterized in that, The second piston pipe (35) is designed to be hollow, and the size is adapted to the fourth piston rod (36).

6. The preparation device for nicotinamide riboside prepared by applying a bio-enzymatic method according to claim 5, characterized in that, A plurality of holes are provided on the reactor (1), and the sizes are respectively adapted to the discharge plate (15) and the handle (11).

7. The preparation device for nicotinamide riboside by applying a biological enzyme method according to claim 1, wherein A scraper (14) is provided on the stirring paddle (13), and the scraper (14) is in contact with the inner wall of the reaction kettle (1).

8. A preparation device for preparing nicotinamide riboside by applying a bio-enzymatic method according to claim 1, characterized in that, Support columns (32) are provided on the storage bucket (31), and both the storage bucket (31) and the water inlet pipe (33) are designed to be hollow.

9. A preparation method for nicotinamide riboside by biocatalytic method, which uses the preparation device for nicotinamide riboside by biocatalytic method described in any one of claims 1-8 above, characterized in that, It includes the following steps; Step 1: Put the mixed solution of nicotinamide and purine nucleoside as raw materials into the first feed bucket (25), control the amount of raw materials entering the reaction kettle (1) by adjusting the second piston rod (271), and put the enzyme solution as the catalyst for the reaction into the second feed bucket (26). Control the amount of catalyst entering the reaction kettle (1) by adjusting the first piston rod (21). Control whether the first insertion rod (27) is connected to the first piston rod (21) through the insertion of the first insertion rod (27) with the first piston rod (21) and the second piston rod (271); Step 2: After the reactants and the catalyst are added to the reaction kettle (1), adjust the connection between the fourth piston rod (36) and the first piston rod (21) by adjusting the insertion of the second insertion rod (28) with the third piston rod (281) and the first piston rod (21). Adjust the amount of the alkaline solution added to the water inlet bucket (34) by adjusting the first piston rod (21), and the adjusted alkaline solution enters the storage bucket (31) through the water inlet pipe (33). Step 3: Drive the rotating rod (12) by starting the motor (16) to drive the stirring paddle (13) to rotate, stir the mixed solution in the reaction kettle (1), and at the same time, the stirring paddle (13) squeezes the limiting ball (38) on the inner wall of the reaction kettle (1), so that the alkaline solution in the storage bucket (31) enters the inside of the reaction kettle (1) through the limiting pipe (37) to neutralize the water generated by the reaction and adjust the pH value of the reaction environment.

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