Percutaneous migration device

Abstract

The invention relates to a percutaneous migration device which comprises a receiving pool for containing human body fluid, an adsorption phase, a link cover, a diffusion film, a sealing piece, a diffusion pool for containing a sample and a top cover. The adsorption phase is placed in the receiving tank; the linking cover is groove-shaped and is hollow in the middle, and the upper end and the lower end of the linking cover are detachably connected to the diffusion tank and the receiving tank respectively; the diffusion film is flatly placed in a groove of the link cover and is fixed through the diffusion cell, and the upper and lower surfaces of the diffusion film are tightly attached to the sample in the diffusion cell and the human body fluid in the receiving cell respectively; a vent hole is formed in the linking cover and used for communicating the receiving tank with ambient air, and the sealing piece detachably blocks the vent hole; the top cover covers the top of the diffusion cell. Through the arrangement of the diffusion film, the sample is in contact with human body fluid only through the film surface, so that chemical substances in the sample are unidirectionally migrated to the human body fluid; and the position of the diffusion film is fixed through the design of the linking cover, so that the adsorption phase is convenient to place and replace. The device is simple to operate and can resist environmental background interference.

Description

Technical Field

[0001] The invention relates to the technical field of percutaneous exposure risk assessment of consumer products to human body, in particular to a percutaneous migration device. Background Art

[0002] As the largest organ of the human body, the skin is constantly exposed to the environment and numerous chemicals. Among them, the contact between the skin and the surface of consumer products is a common skin exposure scenario. Chemical substances in consumer products can enter the human body through the surface of consumer products and through skin exposure. At present, the exposure level of chemical substances in consumer products to human skin can be estimated by wiping the surface of consumer products or artificial immersion, exploring the migration rules of chemical substances in consumer products mediated by sweat, and assessing their transdermal exposure risks to the human body. Among them, the wiping method is affected by the wiping force and the wiping liquid, and can only evaluate the release potential in a very short time. In the artificial sweat immersion method, consumer products are often cut into small pieces or completely crushed for immersion. This method can effectively increase the contact area between consumer products and sweat and shorten the evaluation time. However, this method greatly destroys the structure of consumer products and ignores the actual scenario that the skin only contacts the surface of consumer products.

[0003] Franz transdermal diffusion cells are often used for transdermal testing of drug preparations such as patches, suppositories and semisolid preparations, and can objectively reflect the transdermal release process of preparations. However, this device is often used for polar, high-solubility chemicals, while low-solubility chemicals are not easy to reach the instrument detection limit. Existing studies mostly use the method of adding adsorption phases, such as XAD resin, polydimethylsiloxane, polyethylene, etc., to enrich low-solubility chemicals in environmental water samples, thereby increasing the detection amount of target substances. However, repeated replacement of adsorbents will greatly affect the position of the diffusion membrane in the Franz transdermal diffusion cell. In addition, the chemicals in consumer products include non-polar, low-solubility trace substances, which are susceptible to potential environmental background contamination. Summary of the invention

[0004] In view of the problems existing in the prior art, the purpose of the present invention is to provide a transdermal migration device that is easy to operate, can resist environmental background interference, and can highly simulate the migration process of chemical substances from the surface of consumer products through the skin to human body fluids.

[0005] In order to achieve the above object, the present invention adopts the following technical solution: A transdermal migration device comprises a receiving pool for holding human body fluids, an adsorption phase, a connecting cover, a diffusion membrane, a sealing member, a diffusion pool for placing a sample, and a top cover; The adsorbed phase is placed in the receiving cell; The link cover is in a groove shape with a hollow middle, and its upper and lower ends are detachably connected to the diffusion tank and the receiving tank respectively; The diffusion membrane is placed flat in the groove of the link cover and fixed through the diffusion cell. The upper and lower surfaces of the diffusion membrane are closely attached to the sample in the diffusion cell and the human body fluid in the receiving cell respectively. The link cover is provided with a vent hole for connecting the receiving tank with ambient air, and the sealing member is detachably sealed on the vent hole; The top cover covers the top of the diffusion cell.

[0006] Furthermore, the linking cover is threadedly connected to the diffusion cell and the receiving cell respectively, and the diffusion membrane is fixed by screwing the linking cover and the diffusion cell.

[0007] Furthermore, the lower circumference of the connecting cover is provided with an external thread, and the upper circumference of the receiving pool is provided with an internal thread matching therewith; the upper circumference of the connecting cover is provided with an internal thread, and the lower circumference of the diffusion pool is provided with an external thread matching therewith.

[0008] Furthermore, a small sealing ring is provided on the diffusion membrane, and the diffusion pool is pressed against the small sealing ring.

[0009] Furthermore, a large sealing ring is provided at the connection between the link cover and the receiving tank.

[0010] Furthermore, a ring groove is provided on the circumferential surface of the lower part of the link cover, and the large sealing ring is sleeved in the ring groove.

[0011] Furthermore, the vent hole is a screw hole, the sealing member is a screw, and the screw is threadedly connected to the screw hole.

[0012] Furthermore, the adsorption phase is made of XAD resin, polydimethylsiloxane or polyethylene.

[0013] Furthermore, the diffusion membrane is made of a dialysis membrane or a membrane.

[0014] Furthermore, the receiving tank, the connecting cover, the diffusion tank and the top cover are made of stainless steel, glass or plastic.

[0015] In general, the present invention has the following advantages: The transdermal migration device of the present invention is intended to highly simulate the migration process of chemical substances from the surface of consumer products through the skin to the body fluids of the human body.

[0016] (1) The diffusion membrane is placed in the groove of the connecting cover and fixed by screwing the connecting cover and the diffusion cell. The upper and lower surfaces of the diffusion membrane are tightly fitted with the sample in the diffusion cell and the human body fluid in the receiving cell respectively, so that the sample can fully contact the human body fluid only through the surface of the diffusion membrane, realizing the unidirectional migration of chemical substances in the sample into the human body fluid.

[0017] (2) The receiving cell, the connecting cover and the diffusion cell can be combined by screwing together. The combination can be disassembled by only screwing the receiving cell and the connecting cover without moving the diffusion membrane, which is convenient for recovering and replacing the adsorption phase.

[0018] (3) The vent hole of the connecting cover can be used to conveniently discharge the gas in the receiving pool and extract the human body fluids in the receiving pool, and the vent hole can be blocked by a seal to cut off the connection between the receiving pool and the atmosphere outside the device, thereby ensuring the closedness of the device.

[0019] (4) After assembly, the device is generally closed, which can prevent the body fluid in the receiving pool from being reduced due to leakage or evaporation. At the same time, it can resist environmental background interference and improve the accuracy of detection and analysis. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] Figure 1 A schematic structural diagram of a percutaneous migration device according to the present invention is shown.

[0021] In the figure: 1 is the receiving pool; 2 is the adsorption phase; 3 is the large sealing ring; 4 is the connecting cover, 401 is the groove, 402 is the ring groove, 403 is the screw hole; 5 is the diffusion membrane; 6 is the small sealing ring; 7 is the screw; 8 is the diffusion pool; 9 is the top cover. DETAILED DESCRIPTION

[0022] The present invention will be described in further detail below.

[0023] like Figure 1 As shown, a transdermal migration device includes a receiving pool 1, an adsorption phase 2, a large sealing ring 3, a connecting cover 4, a diffusion membrane 5, a small sealing ring 6, a screw 7, a diffusion pool 8 and a top cover 9.

[0024] The receiving pool 1 is used for containing human body fluids, the connecting cover 4 is used for connecting the receiving pool 1 and the diffusion pool 8, and the diffusion pool 8 is used for placing samples.

[0025] The linking cover 4 is detachably connected to the diffusion cell 8 and the receiving cell 1, preferably by threaded connection. Specifically, the lower circumference of the linking cover 4 is provided with an external thread, and the upper circumference of the receiving cell 1 is provided with an internal thread matching therewith; the upper circumference of the linking cover 4 is provided with an internal thread, and the lower circumference of the diffusion cell 8 is provided with an external thread matching therewith.

[0026] The link cover 4 is in the shape of a groove 401 as a whole, with a hollow middle, an annular groove 402 provided on the lower peripheral surface, and two vent holes symmetrically distributed on the top, preferably screw holes 403.

[0027] The screw hole 403 connects the receiving pool 1 with the ambient air and is used to discharge the gas in the receiving pool 1, so that the human body fluid can fill the receiving pool 1 and fit tightly with the diffusion membrane 5. At the same time, the screw hole 403 can be used as a sampling port for extracting the human body fluid in the receiving pool 1.

[0028] The diffusion membrane 5 is placed flat in the groove 401 of the link cover 4. The diffusion membrane 5 is fixed by screwing the link cover 4 with the diffusion cell 8. The upper and lower surfaces of the diffusion membrane 5 are respectively tightly attached to the sample in the diffusion cell 8 and the human body fluid in the receiving cell 1. The diffusion membrane 5 is made of a dialysis membrane or a skin membrane. The diffusion membrane 5 allows the sample to contact the human body fluid only through the membrane surface, so that the chemical substances in the sample can migrate to the human body fluid in a unidirectional manner. In addition, the position of the diffusion membrane 5 can be fixed by screwing the link cover 4 with the diffusion cell 8; at the same time, the receiving cell 1 and the link cover 4 can be unfastened by twisting, so that the adsorption phase 2 in the receiving cell 1 can be replaced.

[0029] The small sealing ring 6 can be stacked on the diffusion membrane 5, and the diffusion cell 8 is pressed against the small sealing ring 6. The large sealing ring 3 can be nested in the annular groove 402 of the connecting cover 4. Both the small sealing ring 6 and the large sealing ring 3 can be used to enhance the sealing performance of the device to prevent leakage at the connecting cover 4, the diffusion cell 8 and the receiving cell 1.

[0030] The adsorption phase 2 can be placed in the receiving pool 1, and the adsorption phase 2 is made of XAD resin, polydimethylsiloxane or polyethylene. The adsorption phase 2 has an enrichment effect on the target chemical substance, and can increase the detection amount of the target chemical substance.

[0031] The seal is used to block the screw hole 403, and the seal is preferably a screw 7. The screw 7 can cut off the connection between the receiving pool 1 and the atmosphere outside the device, ensure the closedness of the device, resist environmental background interference, and prevent the reduction of human body fluids due to evaporation.

[0032] The top cover 9 covers the top of the diffusion pool 8. After the device is assembled, the whole is closed, which can prevent the body fluid in the receiving pool 1 from being reduced due to leakage or evaporation, and can resist environmental background interference.

[0033] Preferably, the receiving pool 1, the connecting cover 4, the screws 7, the diffusion pool 8 and the top cover 9 are made of stainless steel, glass or plastic.

[0034] When using the sampler, follow these steps: (1) Assemble a transdermal migration device, including a receiving pool 1, an adsorption phase 2, a large sealing ring 3, a connecting cover 4, a diffusion membrane 5, a small sealing ring 6, a screw 7, a diffusion pool 8 and a top cover 9; the receiving pool 1 is used to hold human body fluids, the connecting cover 4 is used to connect the receiving pool 1 and the diffusion pool 8, and the diffusion pool 8 is used to place the sample.

[0035] (2) Fill the receiving pool 1 with human body fluid and plug the screw hole 403 with the screw 7. Place the sample in the diffusion pool 8 and lay it flat on the diffusion membrane 5.

[0036] (3) Installed at 37 o The percutaneous migration test was carried out in an incubator at C.

[0037] (4) Regularly replace the adsorbent and human body fluids. The adsorbent and human body fluids are processed separately and tested and analyzed on instruments such as gas chromatography-mass spectrometry.

[0038] The above embodiments are preferred implementation modes of the present invention, but the implementation modes of the present invention are not limited to the above embodiments. Any other changes, modifications, substitutions, combinations, and simplifications that do not deviate from the spirit and principles of the present invention should be equivalent replacement methods and are included in the protection scope of the present invention.

Claims

1. A percutaneous migration device, characterized in that: It includes a receiving pool for holding human body fluids, an adsorption phase, a connecting cover, a diffusion membrane, a sealing member, a diffusion pool for placing a sample, and a top cover; The adsorbed phase is placed in the receiving cell; The link cover is in a groove shape with a hollow middle, and its upper and lower ends are detachably connected to the diffusion tank and the receiving tank respectively; The diffusion membrane is placed flat in the groove of the link cover and fixed through the diffusion cell. The upper and lower surfaces of the diffusion membrane are closely attached to the sample in the diffusion cell and the human body fluid in the receiving cell respectively. The link cover is provided with a vent hole for connecting the receiving tank with ambient air, and the sealing member is detachably sealed on the vent hole; The top cover covers the top of the diffusion cell.

2. A percutaneous migration device according to claim 1, characterized in that: The connecting cover is respectively threadedly connected to the diffusion pool and the receiving pool, and the diffusion membrane is screwed and fixed with the connection cover and the diffusion pool.

3. A percutaneous migration device according to claim 2, characterized in that: The lower circumference of the connecting cover is provided with external threads, and the upper circumference of the receiving pool is provided with internal threads matching therewith; the upper circumference of the connecting cover is provided with internal threads, and the lower circumference of the diffusion pool is provided with external threads matching therewith.

4. A percutaneous migration device according to claim 1, characterized in that: A small sealing ring is arranged on the diffusion membrane, and the diffusion pool is pressed against the small sealing ring.

5. A percutaneous migration device according to claim 1, characterized in that: A large sealing ring is provided at the connection between the link cover and the receiving tank.

6. A percutaneous migration device according to claim 5, characterized in that: A ring groove is arranged on the peripheral surface of the lower part of the link cover, and a large sealing ring is sleeved in the ring groove.

7. A percutaneous migration device according to claim 1, characterized in that: The vent hole is a screw hole, the sealing element is a screw, and the screw thread is connected to the screw hole.

8. A percutaneous migration device according to claim 1, characterized in that: The adsorption phase is made of XAD resin, polydimethylsiloxane or polyethylene.

9. A percutaneous migration device according to claim 1, characterized in that: The diffusion membrane is made of dialysis membrane or membrane.

10. A percutaneous migration device according to claim 1, characterized in that: The receiving cell, connecting cover, diffusion cell and top cover are made of stainless steel, glass or plastic.

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