A cell centrifugation and sub-packaging device and method

Abstract

The present invention relates to the technical field of cell centrifugation, and discloses a cell centrifugation and dispensing device and method, including a base. A sleeve is rotatably installed on the upper surface of the base. A turntable is fixedly installed on the outer circumferential surface of the sleeve near the top. A plurality of openings are equidistantly penetrated through the outer circumferential surface of the turntable. Two rotating columns are symmetrically and rotatably installed on the inner walls of the opposite sides of each of the plurality of openings. A test tube sleeve is fixedly installed between the adjacent ends of the two rotating columns. After centrifugation of the present invention is completed, the turntable stops rotating. At this time, the test tube sleeve returns to the vertical position. At the same time, the arc-shaped clamp drives the plurality of rubber clamp wheels inside to leave the surface of the centrifugation test tube. When the device releases the limit on the centrifugation test tube, there is no need to manually operate one by one. When batch cell centrifugation and dispensing are carried out, the workload of the operator is reduced, and the efficiency of cell centrifugation and dispensing is increased.

Description

Technical Field

[0001] The present invention relates to the technical field of cell centrifugation, and particularly relates to a cell centrifugation and sub-packaging device and method. Background Art

[0002] A cell centrifugation and sub-packaging device is an auxiliary device specifically used for aseptic experimental operations. It usually includes a centrifuge, centrifuge tubes, a sub-packager, and other necessary components for achieving solid-liquid separation and sub-packaging of cell cultures. Among them, the centrifuge is the core component of the cell centrifugation and sub-packaging device. The centrifuge generates centrifugal force through high-speed rotation, enabling the separation of different components in the cell culture.

[0003] An existing cell centrifugation and sub-packaging device (Publication No.: CN222019764U) has at least the following drawbacks:

[0004] When the above patent is used, after the staff places the centrifuge tube into the placement groove, the fixed mechanism is set to fix the centrifuge tube near the entrance of the placement groove, and after the centrifuge tube is inserted, the separation mechanism is set to clamp and fix the bottom end of the centrifuge tube, thereby preventing the centrifuge tube from shaking during centrifugation. In the above patent, the fixed mechanism and the separation mechanism are set to fix the test tube. However, after the centrifugation work is completed, it is necessary to manually release the fixation of the fixed mechanism and the separation mechanism on the test tube one by one. When performing cell centrifugation and sub-packaging in batches, it will greatly increase the workload of the operator and reduce the efficiency of cell centrifugation and sub-packaging. Summary of the Invention

[0005] The purpose of the present invention is to solve the drawbacks existing in the prior art, and to propose a cell centrifugation and sub-packaging device and method.

[0006] In order to achieve the above purpose, the present invention adopts the following technical solutions:

[0007] A cell centrifugation and sub-packaging device includes a base. A sleeve is rotatably installed on the upper surface of the base. A turntable is fixedly installed on the outer circumferential surface of the sleeve near the top. A plurality of openings are equidistantly penetrated through the outer circumferential surface of the turntable. Two rotating columns are symmetrically rotatably installed on the inner walls of the opposite sides of each opening. A test tube sleeve is fixedly installed between the adjacent ends of the two rotating columns. A centrifuge tube is inserted into the interior of the test tube sleeve. Two supporting blocks are symmetrically fixedly installed on the end face at the top of the test tube sleeve. A top column is slidably inserted into the inner walls of the two supporting blocks. The adjacent end of the top column penetrates the outer surface of the supporting block and is fixedly installed with an arc-shaped clamp. An installation groove is formed on the outer surface of the arc-shaped clamp. A plurality of rubber clamping wheels are equidistantly rotatably installed between the inner walls of the installation groove. The plurality of rubber clamping wheels are in contact with the outer circumferential surface of the centrifuge tube. Arc-shaped grooves are formed on the inner walls of the opposite sides of the opening. The top column is inserted into the arc-shaped groove and is slidably installed with its inner wall.

[0008] As a further solution of the present invention, a retaining ring is fixedly installed on the outer surface of the top column close to the arc groove. A second spring is sleeved on the circumferential outer surface of the top column. The second spring is arranged between the support block and the retaining ring. An arc bevel is provided on the inner wall of the arc groove close to the support block side. A chamfer matching the arc bevel is provided on the end face of the top column. The chamfer on the end face of the top column abuts against the arc bevel. The arc groove and the rotation center of the rotating column are arranged at the same center of a circle.

[0009] As a further solution of the present invention, a fixed column is fixedly installed on the upper surface of the base. The fixed column is arranged inside the sleeve. The top end of the fixed column penetrates through the upper surface of the turntable and is fixedly installed with a fixed disk. A circular groove is opened on the upper surface of the turntable. The fixed disk is arranged inside the circular groove. A driving chamfer is opened on the circumferential outer surface of the fixed disk close to the top end. The top end of the rubber clamping wheel close to the fixed disk penetrates through the upper surface of the arc clamp and is fixedly installed with a fourth gear.

[0010] As a further solution of the present invention, a third gear is rotatably installed on the circumferential outer surface of the arc clamp. The third gear meshes with the fourth gear. A rubber friction wheel is fixedly installed on the upper surface at the middle position of the third gear. The circumferential outer surface of the rubber friction wheel is arranged in a spherical shape. The circumferential outer surface of the rubber friction wheel abuts against the outer surface of the driving chamfer.

[0011] As a further solution of the present invention, a first gear is fixedly installed on the circumferential outer surface of the sleeve close to the bottom end. A second gear is rotatably installed on the upper surface of the base. The second gear meshes with the first gear. A driving motor is fixedly installed on the lower surface of the base. The output end of the driving motor penetrates through the upper surface of the base and is fixedly installed with the rotation center of the second gear.

[0012] As a further solution of the present invention, a plurality of sinking grooves are equidistantly opened in the circumferential direction on the lower surface of the turntable. Slide plates are slidably installed on the inner walls of the plurality of sinking grooves. A dovetail chute is opened on the top wall of the sinking groove. A dovetail slide rail matching the dovetail chute is provided on the upper surface of the slide plate. The dovetail slide rail is slidably installed with the inner wall of the dovetail chute. Arc-shaped chutes are penetrated and opened on the inner walls at the opposite ends of the sinking groove. Plug blocks are slidably installed on the inner walls of the arc-shaped chutes.

[0013] As a further solution of the present invention, two insertion holes are symmetrically opened on the circumferential outer surface of the test tube sleeve. One end of the plug block is inserted into the insertion hole. A driving column is fixedly installed on the upper surface of the other end of the plug block. Two driving grooves are symmetrically penetrated and opened on the upper surface of the slide plate. The driving column is slidably installed with the inner wall of the driving groove. A first spring is fixedly installed on the outer surface of the slide plate far away from the sleeve. The other end of the first spring is fixedly connected with the inner wall of the sinking groove.

[0014] As a further solution of the present invention, an X-direction moving component is slidably installed between the outer surfaces of opposite sides of the base. A Y-direction moving component is provided at the top of the X-direction moving component. A Z-direction moving component is provided on the outer surface of one side of the Y-direction moving component. A dispensing needle is provided at the bottom of the Z-direction moving component.

[0015] A method for using a cell centrifugal dispensing device includes the following steps:

[0016] S1: During use, the operator first inserts the centrifuge tube filled with the cell sample into the inside of the tube sleeve, and then starts the drive motor. The drive motor drives the turntable to rotate. Due to the rotation of the turntable, the slide plate slides outward under the action of centrifugal force. The slide plate drives the plug block to retract and move inside the sunk groove through the drive groove and the drive column, and the plug block leaves the jack. At this time, the tube sleeve is released from the limit.

[0017] S2: Through the tilting movement of the tube sleeve, the rubber friction wheel is driven to abut against the driving chamfer of the fixed disk. At this time, since the turntable and the fixed disk are in relative motion, the rubber friction wheel rotates itself through the friction with the driving chamfer, and drives one of the rubber clamping wheels to rotate through the rubber friction wheel, and the rubber clamping wheel drives the centrifuge tube to rotate itself.

[0018] S3: The turntable drives the tube sleeve to move, and the tube sleeve drives the centrifuge tube to move. Due to the action of centrifugal force, the tube sleeve will tilt at an angle of 30° along the direction of the arc groove, and the samples inside the centrifuge tube are separated from the liquid by centrifugal force to separate cells or cell components of different densities.

[0019] S4: After centrifugation, the X-direction moving component, the Y-direction moving component, and the Z-direction moving component drive the dispensing needle to move above the designated centrifuge tube, and layer-extract and dispense the centrifuged cell liquid inside the centrifuge tube for subsequent detection.

[0020] Compared with the prior art, the present invention has the following beneficial effects:

[0021] 1. After centrifugation, the turntable stops rotating. At this time, the tube sleeve returns to the vertical position. At the same time, the arc clip drives the multiple rubber clamping wheels inside to leave the surface of the centrifuge tube. When the device releases the limit of the centrifuge tube, there is no need to manually operate one by one. When batch centrifuging and dispensing cells, the workload of the operator is reduced, and the efficiency of cell centrifuging and dispensing is increased.

[0022] 2. Through the tilting movement of the test tube sleeve, the rubber friction wheel is driven to abut against the driving chamfer of the fixed disk. At this time, since the turntable and the fixed disk are in relative motion, the rubber friction wheel rotates itself through the friction with the driving chamfer, drives one of the rubber clamping wheels to rotate through the rubber friction wheel, and the rubber clamping wheel drives the centrifuge test tube to rotate. The periodic change of the centrifugal force generated by the rotation of the centrifuge test tube can slightly disturb the precipitate, prevent cells or particles from forming hard lumps at the bottom of the centrifuge test tube, and facilitate subsequent resuspension. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 FIG. is a schematic diagram of the overall structure of a cell centrifugation and dispensing device proposed by the present invention;

[0024] Figure 2 FIG. is a schematic diagram of the bottom view structure of a cell centrifugation and dispensing device proposed by the present invention;

[0025] Figure 3 FIG. is a schematic diagram of the top view structure of the turntable of a cell centrifugation and dispensing device proposed by the present invention;

[0026] Figure 4 FIG. is a schematic diagram of the bottom view structure of the turntable of a cell centrifugation and dispensing device proposed by the present invention;

[0027] Figure 5 FIG. is a schematic diagram of the fixed disk of a cell centrifugation and dispensing device proposed by the present invention;

[0028] Figure 6 FIG. is a schematic diagram of the slide plate of a cell centrifugation and dispensing device proposed by the present invention;

[0029] Figure 7 FIG. is a schematic diagram of the test tube sleeve of a cell centrifugation and dispensing device proposed by the present invention;

[0030] Figure 8 FIG. is a schematic diagram of the arc clamp of a cell centrifugation and dispensing device proposed by the present invention;

[0031] Figure 9 is Figure 5 the partial enlarged view at A in

[0032] Figure 10 is Figure 4 the partial enlarged view at B in.

[0033] In the figure: 1, base; 2, X-direction moving component; 3, Y-direction moving component; 4, Z-direction moving component; 5, sub-packaging needle; 6, sleeve; 7, first gear; 8, second gear; 9, drive motor; 10, fixed disk; 1001, drive chamfer; 1002, fixed column; 11, turntable; 12, test tube sleeve; 1201, support block; 1202, rotating column; 1203, jack; 13, circular groove; 14, opening; 15, counterbore; 16, dovetail chute; 17, slide plate; 18, first spring; 19, plug; 20, drive groove; 21, drive column; 22, arc clamp; 23, mounting groove; 24, rubber clamping wheel; 25, ejector pin; 26, retaining ring; 27, second spring; 28, rubber friction wheel; 29, third gear; 30, fourth gear; 31, arc groove. Detailed implementation manners

[0034] In order to make the technical means, creative features, achieved purposes and functions of the present invention easy to understand, the present invention will be further described below in conjunction with specific implementation manners.

[0035] In the description of the present invention, it should be noted that the orientation or positional relationship indicated by the terms "upper", "lower", "inner", "outer", "front end", "rear end", "both ends", "one end", "the other end", etc. is based on the orientation or positional relationship shown in the drawings. It is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation of the present invention. In addition, the terms "first" and "second" are only used for descriptive purposes and cannot be construed as indicating or implying relative importance.

[0036] In the description of the present invention, it should be noted that unless otherwise clearly specified and limited, the terms "installed", "provided with", "connected", etc. should be understood in a broad sense. For example, "connected" can be a fixed connection, a detachable connection, or an integral connection; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and can be the communication inside two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific situations.

[0037] Refer to Figures 1 - 10, a cell centrifugation and dispensing device, including a base 1. A sleeve 6 is rotatably installed on the upper surface of the base 1. A turntable 11 is fixedly installed on the circumferential outer surface of the sleeve 6 near the top. A plurality of openings 14 are equidistantly penetrated through the circumferential outer surface of the turntable 11. Two rotating columns 1202 are symmetrically rotatably installed on the inner walls of the opposite sides of the plurality of openings 14. A test tube sleeve 12 is fixedly installed between the adjacent ends of the two rotating columns 1202. A centrifuge test tube is inserted into the interior of the test tube sleeve 12. Two support blocks 1201 are symmetrically fixedly installed on the end face of the top of the test tube sleeve 12. A top column 25 is slidably inserted into the inner walls of the two support blocks 1201. The adjacent end of the top column 25 penetrates through the outer surface of the support block 1201 and is fixedly installed with an arc-shaped clamp 22. An installation groove 23 is opened on the outer surface of the arc-shaped clamp 22. A plurality of rubber clamp wheels 24 are equidistantly rotatably installed between the inner walls of the installation groove 23. The plurality of rubber clamp wheels 24 are abutted against the circumferential outer surface of the centrifuge test tube. Arc-shaped grooves 31 are opened on the inner walls of the opposite sides of the opening 14. The top column 25 is inserted into the interior of the arc-shaped groove 31 and slidably installed with its inner wall. A retaining ring 26 is fixedly installed on the outer surface of the top column 25 near the arc-shaped groove 31. A second spring 27 is sleeved on the circumferential outer surface of the top column 25. The second spring 27 is arranged between the support block 1201 and the retaining ring 26. An arc-shaped bevel is arranged on the inner wall of the arc-shaped groove 31 near the support block 1201. A chamfer matching the arc-shaped bevel is arranged on the end face of the top column 25. The chamfer on the end face of the top column 25 abuts against the arc-shaped bevel. The arc-shaped groove 31 and the rotation center of the rotating column 1202 are arranged at the same center of a circle.

[0038] Through the tilting movement of the test tube sleeve 12, the top column 25 slides on the inner wall of the arc-shaped groove 31. When the chamfer on the end face of the top column 25 slides relative to the arc-shaped bevel, it will drive the two arc-shaped clamps 22 to move closer to the centrifuge test tube. The arc-shaped clamp 22 drives the plurality of rubber clamp wheels 24 inside to abut against the outer surface of the centrifuge test tube. The centrifuge test tube is limited by this device to prevent it from shaking during the centrifugation movement. After the centrifugation is completed, the turntable 11 stops rotating. At this time, the test tube sleeve 12 returns to the vertical position. At the same time, the arc-shaped clamp 22 drives the plurality of rubber clamp wheels 24 inside to leave the surface of the centrifuge test tube. When this device releases the limit on the centrifuge test tube, there is no need to manually operate one by one. When batch cell centrifugation and dispensing are carried out, the workload of the operator is reduced and the efficiency of cell centrifugation and dispensing is accelerated.

[0039] In this embodiment, a fixing column 1002 is fixedly installed on the upper surface of the base 1. The fixing column 1002 is arranged inside the sleeve 6. The top end of the fixing column 1002 penetrates through the upper surface of the turntable 11 and is fixedly installed with a fixing plate 10. A circular groove 13 is formed on the upper surface of the turntable 11. The fixing plate 10 is arranged inside the circular groove 13. A driving chamfer 1001 is formed on the outer circumferential surface of the fixing plate 10 near the top end. The top end of the rubber clamping wheel 24 close to the fixing plate 10 penetrates through the upper surface of the arc clamp 22 and is fixedly installed with a fourth gear 30. A third gear 29 is rotatably installed on the outer circumferential surface of the arc clamp 22. The third gear 29 meshes with the fourth gear 30. A rubber friction wheel 28 is fixedly installed on the upper surface at the middle position of the third gear 29. The outer circumferential surface of the rubber friction wheel 28 is spherical. The outer circumferential surface of the rubber friction wheel 28 abuts against the outer surface of the driving chamfer 1001.

[0040] Through the tilting movement of the test tube sleeve 12, the rubber friction wheel 28 is driven to abut against the driving chamfer 1001 of the fixing plate 10. At this time, since the turntable 11 and the fixing plate 10 are in relative motion, the rubber friction wheel 28 rotates by friction with the driving chamfer 1001. One of the rubber clamping wheels 24 is driven to rotate by the rubber friction wheel 28, and the rubber clamping wheel 24 drives the centrifuge tube to rotate. The periodic change of centrifugal force generated by the rotation of the centrifuge tube can slightly disturb the precipitate, preventing cells or particles from forming hard lumps at the bottom of the centrifuge tube and facilitating subsequent resuspension.

[0041] In this embodiment, a first gear 7 is fixedly installed on the outer circumferential surface of the sleeve 6 near the bottom end. A second gear 8 is rotatably installed on the upper surface of the base 1. The second gear 8 meshes with the first gear 7. A driving motor 9 is fixedly installed on the lower surface of the base 1. The output end of the driving motor 9 penetrates through the upper surface of the base 1 and is fixedly installed with the rotation center of the second gear 8.

[0042] The driving motor 9 drives the second gear 8 to rotate. The second gear 8 drives the sleeve 6 to rotate through the first gear 7. The sleeve 6 drives the turntable 11 to rotate. The turntable 11 drives the test tube sleeve 12 to move. The test tube sleeve 12 drives the centrifuge tube to move. Due to the action of centrifugal force, the test tube sleeve 12 will tilt at an angle of 30° along the direction of the arc groove 31. At this time, the samples inside the centrifuge tube are separated from the liquid by centrifugal force, separating cells or cell components of different densities. With this device, it is convenient to perform centrifugal separation on cell samples.

[0043] In this embodiment, a plurality of sunk grooves 15 are equidistantly arranged in the circumferential direction on the lower surface of the turntable 11. Slide plates 17 are slidably installed on the inner walls of the plurality of sunk grooves 15. A dovetail chute 16 is formed in the top wall of the sunk groove 15. A dovetail slide rail matching the dovetail chute 16 is arranged on the upper surface of the slide plate 17. The dovetail slide rail is slidably installed on the inner wall of the dovetail chute 16. Arc-shaped chutes are formed through the inner walls at opposite ends of the sunk groove 15. Plug blocks 19 are slidably installed on the inner walls of the arc-shaped chutes. Two jacks 1203 are symmetrically arranged on the circumferential outer surface of the test tube sleeve 12. One end of the plug block 19 is inserted into the interior of the jack 1203. A driving column 21 is fixedly installed on the upper surface of the other end of the plug block 19. Two driving grooves 20 are symmetrically formed through the upper surface of the slide plate 17. The driving column 21 is slidably installed on the inner wall of the driving groove 20. A first spring 18 is fixedly installed on the outer surface of the slide plate 17 on the side away from the sleeve 6. The other end of the first spring 18 is fixedly connected to the inner wall of the sunk groove 15.

[0044] By rotating the turntable 11, the slide plate 17 slides outward under the action of centrifugal force. The slide plate 17 drives the plug block 19 to retract and move inside the sunk groove 15 through the driving groove 20 and the driving column 21. The plug block 19 leaves the jack 1203. At this time, the test tube sleeve 12 is released from the limit, facilitating the subsequent centrifugal separation of the cell sample inside the centrifuge tube at an inclined angle. When the turntable 11 stops rotating, the slide plate 17 resets under the action of the first spring 18. At this time, the plug block 19 is inserted into the interior of the jack 1203 again. With this device, it is convenient to limit the test tube sleeve 12 to prevent its shaking from affecting the subsequent dispensing of the centrifuged cell sample by the dispensing needle 5.

[0045] In this embodiment, an X-direction moving component 2 is slidably installed between the outer surfaces on opposite sides of the base 1. A Y-direction moving component 3 is arranged on the top of the X-direction moving component 2. A Z-direction moving component 4 is arranged on the outer surface of one side of the Y-direction moving component 3. A dispensing needle 5 is arranged at the bottom of the Z-direction moving component 4.

[0046] The X-direction moving component 2, the Y-direction moving component 3, and the Z-direction moving component 4 drive the dispensing needle 5 to move above the specified centrifuge tube, perform layered extraction on the centrifuged cell fluid inside the centrifuge tube, and dispense it, facilitating subsequent detection.

[0047] A method for using a cell centrifugation and dispensing device includes the following steps:

[0048] S1: During use, the operator first inserts the centrifuge tube filled with cell samples into the inside of the tube sleeve 12, and then starts the drive motor 9. The drive motor 9 drives the turntable 11 to rotate. Due to the rotation of the turntable 11, the slide plate 17 slides outward under the action of centrifugal force. The slide plate 17 drives the insertion block 19 to retract and move inside the sinking groove 15 through the drive groove 20 and the drive post 21, and the insertion block 19 leaves the jack 1203. At this time, the tube sleeve 12 is released from the limit;

[0049] S2: Through the tilting movement of the tube sleeve 12, the rubber friction wheel 28 is driven to abut against the drive chamfer 1001 of the fixed disk 10. At this time, since the turntable 11 and the fixed disk 10 are in relative motion, the rubber friction wheel 28 rotates itself through the friction with the drive chamfer 1001, and drives one of the rubber pinch wheels 24 to rotate through the rubber friction wheel 28, and the rubber pinch wheel 24 drives the centrifuge tube to rotate itself;

[0050] S3: The turntable 11 drives the tube sleeve 12 to move, and the tube sleeve 12 drives the centrifuge tube to move. Due to the action of centrifugal force, the tube sleeve 12 will tilt 30° along the direction of the arc groove 31, and the samples inside the centrifuge tube are separated from the liquid by centrifugal force to separate cells or cell components of different densities;

[0051] S4: After centrifugation, the dispensing needle 5 is driven by the X-direction moving component 2, the Y-direction moving component 3 and the Z-direction moving component 4 to move above the designated centrifuge tube, and the centrifuged cell liquid inside the centrifuge tube is extracted in layers and dispensed, which is convenient for subsequent detection.

[0052] It should be noted that during the use of the present invention, the operator first inserts the centrifuge tube filled with cell samples into the inside of the tube sleeve 12, and then starts the drive motor 9. The drive motor 9 drives the second gear 8 to rotate, the second gear 8 drives the sleeve 6 to rotate through the first gear 7, the sleeve 6 drives the turntable 11 to rotate, the turntable 11 drives the tube sleeve 12 to move, and the tube sleeve 12 drives the centrifuge tube to move. Due to the action of centrifugal force, the tube sleeve 12 will tilt 30° along the direction of the arc groove 31. At this time, the samples inside the centrifuge tube are separated from the liquid by centrifugal force to separate cells or cell components of different densities. The centrifugal separation of cell samples is facilitated by this device;

[0053] The rotation of the turntable 11 causes the slide plate 17 to slide outwards under the action of the centrifugal force, and the slide plate 17 drives the plug block 19 to retract and move inside the sink 15 through the driving groove 20 and the driving column 21, and the plug block 19 leaves the insertion hole 1203. At this time, the test tube sleeve 12 is released from the limit, which is convenient for the subsequent tilt angle to centrifuge the cell sample inside the centrifugal test tube. When the turntable 11 stops rotating, the slide plate 17 is reset under the action of the first spring 18. At this time, the plug block 19 is reinserted into the inside of the insertion hole 1203. The device is convenient for limiting the test tube sleeve 12 to prevent its shaking from affecting the subsequent packaging needle 5 to package the cell sample after centrifugation.

[0054] The tilting movement of the test tube sleeve 12 causes the top column 25 to slide on the inner wall of the arc groove 31. When the chamfer of the end face of the top column 25 slides relative to the arc bevel, it drives the two arc clamps 22 to move closer to the centrifuge test tube. The arc clamps 22 drive the internal multiple rubber clamping wheels 24 to press against the outer surface of the centrifuge test tube. The centrifuge test tube is limited by the device to avoid shaking during centrifugal movement. After the centrifugation is completed, the turntable 11 stops rotating. At this time, the test tube sleeve 12 returns to the vertical position. At the same time, the arc clamps 22 drive the internal multiple rubber clamping wheels 24 to leave the surface of the centrifuge test tube. When the centrifuge test tube is released from the limit by the device, there is no need to manually operate one by one. When batch cell centrifugation is performed, the workload of the operator is reduced and the efficiency of cell centrifugation is accelerated.

[0055] The tilting movement of the test tube sleeve 12 drives the rubber friction wheel 28 to abut against the driving chamfer 1001 of the fixed disk 10. At this time, since the rotating disk 11 and the fixed disk 10 are in relative motion, the rubber friction wheel 28 rotates itself through the friction with the driving chamfer 1001, and the rubber friction wheel 28 drives one of the rubber clamping wheels 24 to rotate, and the rubber clamping wheel 24 drives the centrifugal test tube to rotate. The periodic centrifugal force changes generated by the rotation of the centrifugal test tube can slightly disturb the sedimentation, thereby preventing cells or particles from forming a hard knot at the bottom of the centrifugal test tube, which is convenient for subsequent resuspension;

[0056] After the centrifugation is completed, the X-axis moving component 2, the Y-axis moving component 3 and the Z-axis moving component 4 drive the dispensing needle 5 to move to the top of the designated centrifuge tube, extract the centrifuged cell fluid inside the centrifuge tube in layers, and dispensing it for subsequent detection.

[0057] The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention. It should be understood by those skilled in the art that the present invention is not limited by the above embodiments, and the above embodiments and descriptions are only for explaining the principles of the present invention. Without departing from the spirit and scope of the present invention, the present invention may have various changes and improvements, and these changes and improvements all fall within the scope of the present invention to be protected.

Claims

1. A cell centrifugation and sub-packaging device, comprising a base (1), characterized in that, A sleeve (6) is rotatably mounted on the upper surface of the base (1). A turntable (11) is fixedly mounted on the circumferential outer surface of the sleeve (6) near the top. A plurality of openings (14) are equidistantly penetrated through the circumferential outer surface of the turntable (11). Two rotating columns (1202) are symmetrically rotatably mounted on the inner walls of the opposite sides of each of the plurality of openings (14). A test tube sleeve (12) is fixedly mounted between the adjacent ends of the two rotating columns (1202). A centrifuge test tube is inserted into the test tube sleeve (12). Two support blocks (1201) are symmetrically fixedly mounted on the end face of the top of the test tube sleeve (12). A top column (25) is slidably inserted into the inner walls of the two support blocks (1201). The adjacent end of the top column (25) penetrates through the outer surface of the support block (1201) and is fixedly mounted with an arc-shaped clamp (22). An installation groove (23) is formed on the outer surface of the arc-shaped clamp (22). A plurality of rubber clamping wheels (24) are equidistantly rotatably mounted between the inner walls of the installation groove (23). The outer surfaces of the plurality of rubber clamping wheels (24) are abutted against the circumferential outer surface of the centrifuge test tube. Arc-shaped grooves (31) are formed on the inner walls of the opposite sides of the opening (14). The top column (25) is inserted into the arc-shaped groove (31) and is slidably mounted with its inner wall. A retaining ring (26) is fixedly mounted on the outer surface of the top column (25) near the arc-shaped groove (31). A second spring (27) is sleeved on the circumferential outer surface of the top column (25). The second spring (27) is arranged between the support block (1201) and the retaining ring (26). An arc-shaped bevel is arranged on the inner wall of the arc-shaped groove (31) near the support block (1201). A chamfer matching the arc-shaped bevel is arranged on the end face of the top column (25). The chamfer on the end face of the top column (25) is abutted against the arc-shaped bevel. The arc-shaped groove (31) and the rotation center of the rotating column (1202) are arranged at the same center of a circle. A fixed column (1002) is fixedly mounted on the upper surface of the base (1). The fixed column (1002) is arranged inside the sleeve (6). The top of the fixed column (1002) penetrates through the upper surface of the turntable (11) and is fixedly mounted with a fixed disk (10). A circular groove (13) is formed on the upper surface of the turntable (11). The fixed disk (10) is arranged inside the circular groove (13). A driving chamfer (1001) is formed on the circumferential outer surface of the fixed disk (10) near the top. The top of the rubber clamping wheel (24) near the fixed disk (10) penetrates through the upper surface of the arc-shaped clamp (22) and is fixedly mounted with a fourth gear (30). A third gear (29) is rotatably mounted on the circumferential outer surface of the arc-shaped clamp (22). The third gear (29) is meshed with the fourth gear (30). A rubber friction wheel (28) is fixedly mounted on the upper surface of the middle position of the third gear (29). The circumferential outer surface of the rubber friction wheel (28) is spherical. The circumferential outer surface of the rubber friction wheel (28) is abutted against the outer surface of the driving chamfer (1001).

2. The cell centrifugation and sub-packaging device according to claim 1, characterized in that, A first gear (7) is fixedly installed on the circumferential outer surface of the sleeve (6) near the bottom end. A second gear (8) is rotatably installed on the upper surface of the base (1). The second gear (8) meshes with the first gear (7). A driving motor (9) is fixedly installed on the lower surface of the base (1). The output end of the driving motor (9) penetrates through the upper surface of the base (1) and is fixedly installed at the rotation center of the second gear (8).

3. The cell centrifugation and aliquoting device according to claim 1, characterized in that, A plurality of sinking grooves (15) are equidistantly formed in the circumferential direction on the lower surface of the turntable (11). Slide plates (17) are slidably installed on the inner walls of the plurality of sinking grooves (15). A dovetail chute (16) is formed in the top wall of the sinking groove (15). A dovetail slide rail matching the dovetail chute (16) is arranged on the upper surface of the slide plate (17). The dovetail slide rail is slidably installed on the inner wall of the dovetail chute (16). Arc-shaped chutes are formed through the inner walls at the opposite ends of the sinking groove (15). Plug blocks (19) are slidably installed on the inner walls of the arc-shaped chutes.

4. A cell centrifugation and sub-packaging device according to claim 3, characterized in that Two jacks (1203) are symmetrically formed on the circumferential outer surface of the test tube sleeve (12). One end of the plug block (19) is inserted into the interior of the jack (1203). A driving column (21) is fixedly installed on the upper surface of the other end of the plug block (19). Two driving grooves (20) are symmetrically formed through the upper surface of the slide plate (17). The driving column (21) is slidably installed on the inner wall of the driving groove (20). A first spring (18) is fixedly installed on the outer surface of the slide plate (17) away from the sleeve (6). The other end of the first spring (18) is fixedly connected to the inner wall of the sinking groove (15).

5. The cell centrifugation and aliquoting device according to claim 1, wherein An X-direction moving component (2) is slidably installed between the outer surfaces on the opposite sides of the base (1). A Y-direction moving component (3) is arranged on the top of the X-direction moving component (2). A Z-direction moving component (4) is arranged on the outer surface of one side of the Y-direction moving component (3). A dispensing needle (5) is arranged at the bottom of the Z-direction moving component (4).

6. A method for using a cell centrifugation and aliquoting device, characterized in that, Adopting a cell centrifugal dispensing device according to any one of claims 1-5, comprising the following steps: S1: During use, the operator first inserts the centrifugal test tube filled with the cell sample into the interior of the test tube sleeve (12), and then starts the driving motor (9). The driving motor (9) drives the turntable (11) to rotate. Due to the rotation of the turntable (11), the slide plate (17) slides outward under the action of centrifugal force. The slide plate (17) drives the plug block (19) to retract and move inside the sinking groove (15) through the driving groove (20) and the driving column (21). The plug block (19) leaves the jack (1203). At this time, the test tube sleeve (12) is released from the limit; S2: Through the tilting movement of the test tube sleeve (12), the rubber friction wheel (28) is driven to abut against the driving chamfer (1001) of the fixed disk (10). At this time, since the turntable (11) and the fixed disk (10) are in relative motion, the rubber friction wheel (28) rotates itself through the friction with the driving chamfer (1001), and drives one of the rubber clamping wheels (24) to rotate through the rubber friction wheel (28), and the rubber clamping wheel (24) drives the centrifuge test tube to rotate self - revolve; S3: The turntable (11) drives the test tube sleeve (12) to move, and the test tube sleeve (12) drives the centrifuge test tube to move. Due to the action of centrifugal force, the test tube sleeve (12) will tilt at an angle of 30° along the direction of the arc groove (31), and the samples inside the centrifuge test tube are separated from the liquid by centrifugal force into cells or cell components with different densities; S4: After centrifugation, the dispensing needle (5) is driven by the X - direction moving component (2), Y - direction moving component (3) and Z - direction moving component (4) to move above the designated centrifuge test tube, and the centrifuged cell liquid inside the centrifuge test tube is extracted in layers and dispensed, which is convenient for subsequent detection.

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