Efficient penthorum chinense pursh breeding method based on rhizome cuttage

Abstract

The invention discloses an efficient penthorum chinense pursh breeding method based on rhizome cuttage, and relates to the technical field of penthorum chinense pursh breeding and planting, the efficient penthorum chinense pursh breeding method based on rhizome cuttage comprises the following steps: (1) selecting penthorum chinense pursh underground rhizome as a breeding material, and performing cutting treatment to obtain penthorum chinense pursh cutting slips; (2) performing cuttage and environment management on the penthorum chinense pursh cutting slips, and cultivating to obtain penthorum chinense pursh seedlings. According to the method, an efficient, rapid and stable penthorum chinense pursh seedling breeding mode is achieved, the problems that seed breeding is long in period, low in survival rate, complex in management and the like are solved, the method has important industrial popularization value, and the planting efficiency and economic benefits of penthorum chinense pursh can be greatly improved.

Description

Technical Field

[0001] The invention relates to the technical field of breeding and planting of yellow grass, and in particular to an efficient breeding method of yellow grass based on rhizome cuttings. Background Art

[0002] At present, the seedling breeding of yellow grass in field production mainly relies on seed seedling technology. Its sowing is usually arranged from November to December each year, and transplanting is carried out in mid-to-late April of the following year. However, this breeding method has many challenges. First, its seedling breeding cycle is relatively long, taking as long as 5 months, which limits production efficiency and makes it difficult to meet the market demand for rapid expansion.

[0003] The seeds of the yellow grass are tiny, only between 400-600μm, and the seedlings grow slowly, which makes them extremely sensitive to environmental factors (such as temperature, humidity, soil conditions, etc.), making management more difficult. In addition, the seed germination rate is restricted by many factors, and the survival rate of seedlings is low, further exacerbating the complexity and cost burden of the seedling raising process.

[0004] Through an in-depth analysis of existing relevant patent technologies, the inventors found that although there are patents such as "A method for cultivating seedlings of yellow grass" (CN202311598257.7), "A method for planting yellow grass" (CN202110618758.1) and "A method for planting yellow grass" (CN201710315027.3) that have proposed to improve the success rate of seed propagation by optimizing the seedling matrix, controlling environmental conditions or improving fertilizer and water management, the following core problems are still faced:

[0005] 1. Long seedling raising cycle: Seed breeding takes at least 5 months from sowing to transplanting stage. This long cycle seriously restricts production efficiency and makes it difficult to meet the urgent needs of rapid expansion.

[0006] 2. The germination rate of seeds is easily affected by the environment: Due to its small nature, the seeds of the yellow grass have extremely high requirements for environmental conditions such as water, temperature, and light. Any slight environmental fluctuations may lead to a significant drop in the germination rate.

[0007] 3. Seedlings grow slowly and are difficult to manage: Seedlings grow weakly in the early stages and have low disease resistance, requiring meticulous water and fertilizer management to support them, which undoubtedly increases the difficulty and cost of field management.

[0008] 4. Unstable seedling quality: Due to differences between individual seeds and uncontrollable environmental factors, the seedlings bred vary in size, which in turn affects their growth performance after field transplanting.

[0009] In addition, it is worth noting that as a plant whose whole plant is used as medicine, the underground rhizomes of the herb are usually abandoned during the traditional harvesting process and are not fully utilized, resulting in a waste of resources. It is undoubtedly a pity that the existing technology ignores the development and utilization of the underground rhizomes as a potential resource in the planting and seedling raising process of the herb.

[0010] In summary, the seedling breeding technology of Rhizoma Cibotii urgently needs innovation to solve the current problems such as long seedling raising cycle, germination rate being greatly affected by the environment, slow seedling growth and difficult management, unstable seedling quality and low utilization rate of underground rhizome resources. Summary of the invention

[0011] In order to solve the above problems, the present invention provides a highly efficient breeding method of yellow grass based on rhizome cuttings.

[0012] The present invention provides a method for efficiently breeding yellow grass based on rhizome cuttings, and the method comprises the following steps:

[0013] (1) selecting underground rhizomes of the herb as breeding materials and performing cutting processing to obtain herb cuttings;

[0014] (2) The cuttings of the yellow grass are subjected to cutting and environmental management to cultivate the yellow grass seedlings.

[0015] Furthermore, the step of selecting underground rhizomes of the yellow grass as breeding materials includes the following processes:

[0016] After harvesting the yellow grass, select healthy underground rhizomes without diseases and insect pests for preparation of cuttings.

[0017] Furthermore, rhizomes with buds and roots are selected, cut to a length of 4 to 6 cm, and 1.5 to 2 cm of the roots are retained for use as cuttings.

[0018] Furthermore, the step of grafting and managing the cuttings of the yellow grass to obtain the yellow grass seedlings includes the following process:

[0019] (1) Prepare the seedling medium;

[0020] (2) Place the rhizome cuttings horizontally on the surface of the seedling medium and cover them with a 1-2 cm thick seedling medium;

[0021] (3) After sufficient watering to establish roots, place the seedling container in a water tray with a water depth of 4 cm. When the water evaporates to the bottom, add water again to 4 cm to form a dry and wet cycle;

[0022] (4) Cover the seedling container with a transparent cover, leaving a ventilation gap between the cover and the container;

[0023] (5) The seedling system was placed in an environment with a temperature of 25°C, 18 hours of light per day, 6 hours of darkness, and a light intensity of 8000 Lux for cultivation.

[0024] Furthermore, sprouts can be seen on the cuttings within 5 days after cutting, and healthy seedlings of the yellow grass with a plant height of 5 to 10 cm and a well-developed root system can be obtained within 14 days.

[0025] Furthermore, the seedling medium is pure peat soil.

[0026] Furthermore, the water depth fluctuation in the tray is controlled within the range of 4±0.5 cm.

[0027] Furthermore, the survival rate of rhizome cuttings was 100%.

[0028] The above technical solution provided by the embodiment of the present invention has at least the following advantages compared with the prior art:

[0029] The embodiment of the present invention provides an efficient breeding method of yellow grass based on rhizome cuttings. The present invention innovatively uses the underground rhizomes of yellow grass as the core material for seedling breeding. By optimizing the rhizome cutting seedling breeding method, a high survival rate of seedlings, rapid growth and a strong and developed root system are achieved. Compared with traditional seed breeding, this patented technology exhibits the following significant advantages:

[0030] 1. The seedling raising period is significantly shortened: The present invention adopts rhizome cuttings for breeding, and the seedlings can emerge in just 5 days, and the seedling length can reach 5-10 cm in 14 days. Compared with the 5 months required for traditional seed seedling raising, this speed greatly shortens the seedling raising period and improves production efficiency.

[0031] 2. High survival rate of seedlings: Seed propagation is easily affected by environmental factors, and the germination rate is unstable. However, the present invention utilizes rhizomes for breeding, and the seedlings are strong and not limited by the seed germination rate, and the survival rate is as high as 100%, ensuring the success rate of seedling cultivation.

[0032] 3. Underground rhizome resources are fully utilized: In traditional harvesting methods, underground rhizomes of yellow grass are usually abandoned, resulting in a waste of resources. The present invention successfully uses underground rhizomes as seedling materials, and through optimizing rhizome processing and cutting technology, effectively activates its regeneration ability, making it an efficient source of seedlings and improving resource utilization.

[0033] 4. Maintain excellent traits and ensure genetic stability: During seed propagation, the traits of the offspring may vary, affecting product quality. This patent adopts asexual propagation (root and stem cuttings), which can directly maintain the excellent traits of the mother plant, avoid genetic variation problems, and ensure the genetic stability of the seedlings. This is particularly suitable for breeding specific excellent strains or high-efficacy varieties to ensure the consistency and quality of the yellow grass in large-scale planting.

[0034] 5. Suitable for large-scale production and improve the level of industrialization: rhizome propagation does not require fine management of seed germination conditions, and can be used for large-scale seedling cultivation under artificial control environments, greatly improving production efficiency. This technology is suitable for modern agriculture, facility agriculture, etc., and helps to improve the industrialization level of yellow grass.

[0035] 6. Well-developed root system, enhanced survival rate after transplantation: Compared with the problem of weak seedlings in the seedling stage, the seedlings cultivated by this patented method have more developed and robust root systems, are more adaptable after transplanting, and significantly improve the success rate of field planting.

[0036] In summary, the patented technology for raising seedlings of Rhizoma Chrysanthemi in the current market still mainly relies on seed breeding, and has significant shortcomings such as long seedling raising cycle, unstable seedling quality and high management cost. At the same time, the traditional harvesting method only takes the above-ground part, and the utilization rate of underground rhizomes is low, resulting in waste of resources. The patented method is based on rhizome cutting propagation, which not only shortens the seedling raising time and improves the survival rate, but also makes full use of the underground rhizome resources of Rhizoma Chrysanthemi to achieve a more efficient seedling raising method. More importantly, the excellent traits of specific varieties are maintained through asexual reproduction, avoiding the problem of genetic variation, so that Rhizoma Chrysanthemi has higher stability in terms of medicinal ingredients, stress resistance and growth potential. Therefore, the present invention has great application value and competitiveness in modern Chinese medicinal material planting, large-scale seedling raising and industrial promotion. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] The accompanying drawings, which are incorporated in and constitute a part of this specification, illustrate embodiments consistent with the invention and, together with the description, serve to explain the principles of the invention.

[0038] In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required for use in the embodiments or the description of the prior art will be briefly introduced below. Obviously, for ordinary technicians in this field, other drawings can be obtained based on these drawings without paying any creative labor.

[0039] Figure 1 This is a picture of the field situation after the leaves of the yellow grass are harvested in Example 1 of the present invention.

[0040] Figure 2 This is the underground rhizome and root diagram of the yellow grass in Example 1 of the present invention.

[0041] Figure 3 This is a diagram of cuttings of yellow grass in Example 1 of the present invention.

[0042] Figure 4 This is a horizontal view of the rhizome of the yellow grass in Example 1 of the present invention.

[0043] Figure 5 This is a diagram of the rhizome reproduction and seedling emergence of the yellow grass in Example 1 of the present invention.

[0044] Figure 6 This is a diagram of the rhizomes of the yellow grass being kept in water in Example 1 of the present invention.

[0045] Figure 7 This is a diagram of seedlings propagated by rhizomes of Herba Chrysanthemi in Example 1 of the present invention.

[0046] Figure 8 This is a diagram of breeding healthy seedlings from the rhizomes of the yellow grass in Example 1 of the present invention.

[0047] Fig. 9 Illustration of the aboveground part of the yellow grass with buds on the stems.

[0048] Fig.10 This picture shows the withering of the aboveground part of the yellow grass stems with buds after cuttings.

[0049] Fig.11 A picture of the rhizome cuttings with buds from the underground part of yellow grass.

[0050] Fig.12 To drive away the rhizomes of yellow grass, Fig.12 center left) and root ( Fig.12 Middle right) Figure.

[0051] Fig.13 Diagram of field seedling propagation for yellow grass seeds.

[0052] Fig.14 Picture of field breeding of yellow grass seeds.

[0053] Fig.15 Picture of breeding seedlings from rhizomes of yellow grass.

[0054] Fig.16 This is a diagram of the germination process of yellow grass seeds.

[0055] Fig.17 This is a diagram of the tissue culture of yellow grass seeds. DETAILED DESCRIPTION

[0056] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0057] Unless otherwise specified, various raw materials, reagents, instruments and equipment used in the present invention can be purchased from the market or prepared by existing methods.

[0058] The technical solution provided by the present invention is as follows:

[0059] The present invention provides a method for efficiently breeding yellow grass based on rhizome cuttings, and the method comprises the following steps:

[0060] (1) selecting underground rhizomes of the herb as breeding materials and performing cutting processing to obtain herb cuttings;

[0061] (2) The cuttings of the yellow grass are subjected to cutting and environmental management to cultivate the yellow grass seedlings.

[0062] The embodiment of the present invention provides an efficient breeding method of yellow grass based on rhizome cuttings. This patented technology realizes an efficient, fast and stable breeding method of yellow grass seedlings, overcomes the problems of long seed propagation cycle, low survival rate, complex management, etc., has important industrial promotion value, and can greatly improve the planting efficiency and economic benefits of yellow grass.

[0063] Compared with the traditional method of breeding yellow grass seeds, this patented technology has the following significant beneficial effects:

[0064] (1) The seedling raising cycle is greatly shortened

[0065] The traditional seed seedling raising cycle is as long as 5 months. This technology uses rhizome cuttings, which can produce seedlings in 5 days and can reach 5-10cm in 14 days, greatly improving the breeding efficiency.

[0066] (2) High survival rate of seedlings

[0067] The use of rhizome asexual reproduction avoids the problem of low seed germination rate (germination rate 33%-48.89%), and the survival rate reaches 100%, ensuring healthy growth in the seedling stage.

[0068] (3) Well-developed root system improves transplant survival rate

[0069] By optimizing water management and substrate selection, the cultivated seedlings have well-developed root systems, strong stress resistance, higher survival rates after transplanting, and stronger adaptability.

[0070] (4) Stabilize heredity and maintain excellent variety characteristics

[0071] Asexual reproduction can maintain the excellent traits of the mother plant, such as high medicinal ingredients, strong disease resistance, and good stress resistance, and avoid the trait variation of seed reproduction.

[0072] (5) Make full use of underground rhizome resources and reduce waste

[0073] Traditional harvesting only uses the above-ground part of the yellow grass, but this technology uses underground rhizomes to breed new seedlings, improving resource utilization and reducing agricultural waste.

[0074] (6) Suitable for large-scale production and improving the level of industrialization

[0075] The short seedling raising period and high survival rate enable this technology to be promoted on a large scale under an artificially controlled environment, which will contribute to the modernization and standardization of yellow grass cultivation.

[0076] It should be noted that the component raw materials involved in the efficient breeding method of Rhizoma Cibotii based on rhizome cuttings provided in the embodiment of the present invention, unless otherwise specified or specified, can be directly commercially available products or homemade using existing public preparation methods; at the same time, the steps and parameters involved, unless otherwise specified or specified, can be carried out according to the process steps and parameters disclosed in the prior art or directly using existing equipment according to the instruction manual, and the present invention document will not repeat them one by one.

[0077] The present invention will be further described below in conjunction with specific examples. It should be understood that these examples are intended only to illustrate the present invention and are not intended to limit the scope of the present invention. The experimental methods in the following examples that do not specify specific conditions are usually measured according to national standards. If there is no corresponding national standard, then the conditions recommended by the manufacturer are followed.

[0078] Example 1

[0079] This example provides an efficient breeding method for yellow grass based on rhizome cuttings, comprising the following steps:

[0080] 1. Cutting materials: Figure 1 and Figure 2 As shown, the disease-free root of the yellow grass after harvesting is taken;

[0081] 2. Cuttings processing: Figure 3 As shown, select rhizomes with buds and roots, cut the rhizomes to about 4-6 cm, and trim the roots to about 1.5-2 cm as cuttings;

[0082] 3. Cutting and management: Figure 4 , Figure 5 , Figure 6 , Figure 7 and Figure 8As shown, pure grass carbon soil is used as the matrix, and a 7cm*7cm*8cm flower pot is used as a seedling pot. The rhizome cuttings are placed horizontally on the matrix and then covered with 1cm-2cm of soil; water thoroughly; the flower pot tray is filled with water about 4cm deep, and water is added to 4cm when the water reaches the bottom, and this is repeated when it is dry and wet; the flower pot is covered with a stuffy cover to keep moisture, leaving gaps for ventilation; the culture environment is 25℃, 18 hours of light + 6 hours of darkness, and the light intensity during light is 8000Lu. Under this condition, the yellow grass can emerge in 5 days, and the yellow grass seedlings can reach 5cm-10cm in height in 14 days, and the root system is well developed and strong.

[0083] Test Example 1

[0084] On the basis of the above-mentioned Example 1, this example screens and evaluates various experimental parameters in the efficient breeding method of Herba Lycopodii based on rhizome cuttings provided by the present invention.

[0085] (1) Cutting materials: rhizomes with buds and stems with buds

[0086] The study investigated the seedling propagation of two materials, the aboveground stems with buds and the underground rhizomes of Ganhuangcao. The results showed that the survival rate of the aboveground stems with buds was low, 0%, while the survival rate of the rhizomes was 100%. Fig. 9 , Fig.10 and Fig.11 shown.

[0087] (2) Cuttings processing: root pruning and root retention

[0088] The experiment investigated the seedling propagation of the underground rhizomes of the yellow grass under two treatments: root pruning and root retention. The results showed that the root pruning seedling rate was 70% and the root retention seedling rate was 50% after 2 weeks of root pruning. Fig.12 shown.

[0089] (3) Culture temperature: 25℃ and 15℃

[0090] The experiment investigated the effects of cultivation conditions A: 25℃, 18 hours of light + 6 hours of darkness, and 8000Lu of light intensity during light; B: 15℃, 18 hours of light + 6 hours of darkness, and 8000Lu of light intensity during light on the seedling propagation of underground rhizomes of the yellow grass. The results showed that the rhizomes of the yellow grass germinated quickly and grew rapidly under 25℃ conditions. The yellow grass germinated in 5 days, and the yellow grass seedlings could reach 5cm-10cm in height in 14 days, with a strong and developed root system. At 15℃, the seedlings germinated in 16 days, and the yellow grass seedlings were 5-10cm high in 49 days.

[0091] Test Example 2

[0092] On the basis of Example 1, this example compares the efficient breeding method of Herba Lycopodii based on rhizome cuttings provided by the present invention with other methods for breeding Herba Lycopodii seedlings. The experiment investigates and compares the seed sowing technology commonly used in field production and the rhizome seedling breeding technology of the present technology. The seed seedling breeding speed is slow.

[0093] 1) Seed and seedling propagation during field production: Field production is sown from late November to late December, and the seeds are mixed with plant ash and spread on the bed surface. The bed ditch is flooded, the bed surface is wet but not waterlogged, and a transparent film is set up to cover the arch. The film is removed above 20℃. The seedlings are about 15cm high in mid-to-late April. The seedling raising cycle is long (5 months). Fig.13 and Fig.14 shown.

[0094] 2) This technology is used to propagate the seedlings of the yellow grass through rhizomes: the seedlings can emerge in 5 days, the emergence rate is as high as 100%, and the growth is rapid. In 14 days, the seedlings of the yellow grass can reach 5cm-10cm in height, and the root system is well developed and strong. Fig.15 shown.

[0095] 3) Seed tissue culture: The seeds of the yellow grass are very small, about 400-600um long. After sowing in tissue culture MS medium, they begin to germinate on the 7th day, and the cotyledons begin to unfold on the 15th day. The seedlings are thin and weak, growing slowly, and are about 1.5cm-3cm high after 1 month. Fig.16 and Fig.17 shown.

[0096] In summary, the embodiments of the present invention provide an efficient breeding method of yellow grass based on rhizome cuttings. The present invention realizes an efficient, fast and stable breeding method of yellow grass seedlings, overcomes the problems of long seed propagation cycle, low survival rate, complex management, etc., has important industrial promotion value, and can greatly improve the planting efficiency and economic benefits of yellow grass.

[0097] Various embodiments of the present invention may be presented in the form of a range; it should be understood that the description in the form of a range is only for convenience and brevity, and should not be understood as a rigid limitation on the scope of the present invention; therefore, the range description should be considered to have specifically disclosed all possible sub-ranges and single numerical values ​​within the range. For example, the range description from 1 to 6 should be considered to have specifically disclosed sub-ranges, such as from 1 to 3, from 1 to 4, from 1 to 5, from 2 to 4, from 2 to 6, from 3 to 6, etc., as well as single numbers within the range, such as 1, 2, 3, 4, 5 and 6, which apply regardless of the range. In addition, whenever a numerical range is indicated herein, it is meant to include any cited number (fractional or integer) within the indicated range.

[0098] The foregoing is merely a specific embodiment of the present invention, which enables those skilled in the art to understand or implement the present invention. Various modifications to these embodiments will be apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the present invention. Therefore, the present invention will not be limited to the embodiments shown herein, but rather to the widest scope consistent with the principles and novel features claimed herein.

Claims

1. A highly efficient breeding method of yellow grass based on rhizome cuttings, characterized in that: The following steps are involved: (1) selecting underground rhizomes of the herb as breeding materials and performing cutting processing to obtain herb cuttings; (2) The cuttings of the yellow grass are subjected to cutting and environmental management to cultivate the yellow grass seedlings.

2. The method according to claim 1, characterized in that The step of selecting underground rhizomes of yellow grass as breeding materials comprises: After harvesting the yellow grass, select healthy underground rhizomes without diseases and insect pests for preparation of cuttings.

3. The method according to claim 1, characterized in that The cuttings processing comprises the following process: Select rhizomes with buds and roots, cut them into a length of 4 to 6 cm, and retain 1.5 to 2 cm of roots to use as cuttings.

4. The method according to claim 1, characterized in that: The cutting and management steps of the cuttings include: (1) Prepare the seedling medium; (2) Place the rhizome cuttings horizontally on the surface of the seedling medium and cover them with a 1-2 cm thick seedling medium; (3) After sufficient watering to establish roots, place the seedling container in a water tray with a water depth of 4 cm. When the water evaporates to the bottom, add water again to 4 cm to form a dry and wet cycle; (4) Cover the seedling container with a transparent cover, leaving a ventilation gap between the cover and the container; (5) The seedling system was placed in an environment with a temperature of 25°C, 18 hours of light per day, 6 hours of darkness, and a light intensity of 8000 Lux for cultivation.

5. The method according to claim 4, characterized in that The cuttings can be seen sprouting within 5 days after cutting, and healthy seedlings of yellow grass with a height of 5 to 10 cm and a well-developed root system can be obtained within 14 days.

6. The method according to claim 4, characterized in that The seedling raising medium is pure peat soil.

7. The method according to claim 4, characterized in that The water depth fluctuation in the tray is controlled within the range of 4±0.5 cm.

8. The method according to claim 4, characterized in that The survival rate of rhizome cuttings is 100%.

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