Sex attractant for mipolygus lucorum and application of sex attractant

Abstract

The invention discloses a sex attractant for mipolygus lucorum and application of the sex attractant in a Xinjiang cotton field. The sex attractant for the mipolygus lucorum is prepared from the following components: hexyl acetate and crotonyl butyrate. The optimal mass ratio of the hexyl acetate to the crotonyl butyrate is 1: 1, and the recommended dosage is not less than 5 mg / lure. The formula of the sex attractant for the mipolygus lucorum, provided by the invention, has the advantages of simplicity and convenience in preparation, small dosage, convenience in use and the like, and can accurately monitor the occurrence trend of a cotton field in Xinjiang. When the sex attractant is applied in the field, the sex attractant is matched with a corresponding trap to be used, population dynamics of the mipolygus lucorum can be monitored and forecasted, scientific use of chemical pesticides is guided, and the sex attractant has important significance on protection of natural enemies and comprehensive treatment of cotton field pests.

Description

Technical Field

[0001] The invention relates to the technical field of biological control of agricultural pests, in particular to a sex attractant for Microhylidae heteropalpus and an application thereof. Background Art

[0002] As an important economic crop in my country, cotton is harmed by a variety of pests, which seriously affects the yield and quality of cotton. Using natural enemies to control pests is an environmentally safe and effective control method. However, the number of natural enemies in the natural state is relatively scattered and is easily affected by adverse stress, which weakens the ability of natural enemies to control pest populations, threatening the safety of agricultural production. Natural enemy attractants can improve the utilization rate of natural enemies, optimize the local natural enemy population, and improve the effect of pest control. Chemical pheromones play an important role in the behavior and physiology of natural enemy insects. Among them, insect sex pheromones can regulate insect mating, aggregation behavior and insect population size. Attractants based on sex pheromones are more efficient, non-toxic, and pollution-free, and are widely used. However, there is little research on sex pheromones of predatory natural enemy insects. Therefore, the discovery and preparation of sex attractants for predatory natural enemy insects is of great significance for monitoring the number of natural enemy populations, determining the occurrence period of natural enemies, protecting natural enemies, and improving the effect of pest control.

[0003] Campylomma diversicornis Reuter is one of the dominant predatory natural enemies in cotton fields in Xinjiang, my country. Adults and older nymphs of Campylomma diversicornis Reuter can prey on eggs and newly hatched larvae of cotton bollworm, cotton spider mites, cotton aphids, thrips and other pests in cotton fields. It is the main predatory natural enemy of cotton bollworm and thrips in the middle and late stages of cotton fields in Xinjiang. Therefore, Campylomma diversicornis Reuter has great application potential in green pest control in cotton fields.

[0004] The sex attractant of the micro-blight bug of the present invention can be used for monitoring, forecasting and protecting the population of the micro-blight bug in Xinjiang cotton fields, and is also of great value in regulating the spatial distribution of the micro-blight bug population and controlling the number of pests in Xinjiang cotton fields. Summary of the invention

[0005] The invention aims to provide a sex attractant for Lygus heteropalpis and application thereof, by which Lygus heteropalpis can be lured, thereby playing a role in population dynamics monitoring and forecasting.

[0006] To achieve the above object, the present invention provides a sex attractant for Microhylidae heteropalpis, which is composed of the following two components according to their respective weight percentages: 10-90% hexyl acetate and 10-90% crotonyl butyrate, and the sum of the weight percentages of the two components is 100%.

[0007] Optionally, the sex attractant for Lymantria heteropalpis is composed of the following two components according to their respective weight percentages: 30-80% hexyl acetate and 20-70% crotonyl butyrate, and the sum of the weight percentages of the two components is 100%.

[0008] Preferably, the sex attractant for Lymantria heteropalpis is composed of the following two components according to their respective weight percentages: 50% hexyl acetate and 50% crotonyl butyrate, and the sum of the weight percentages of the two components is 100%.

[0009] Furthermore, a method for preparing a sex attractant for a micro-thorny stink bug is provided: the sex pheromone components are placed in vegetable oil according to their respective weight percentages and mixed evenly, and then injected into a slow-release carrier polyethylene bottle, and sealed to prepare a sex attractant. Each sex attractant core is loaded with more than 5 mg of the sex pheromone active ingredient, and its field validity period can reach more than one month. When used in the field, the sex attractant is placed on a common pest trapping device such as a triangular trap, a barrel trap, and a boat trap, and the trap is hung 20-40 cm above the cotton canopy. DETAILED DESCRIPTION

[0010] Through preliminary analysis of female-specific volatiles of male and female A. heteropalpis, and repeated verification through electrophysiological tests and field experiments, we finally identified the sex pheromone components of A. heteropalpis, and developed a sex attractant with significant luring effect.

[0011] In order to make the purpose, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below in conjunction with specific experiments.

[0012] The trapping experiment used the lure core as described above, that is, a polyethylene bottle was used as a slow-release carrier, hexyl acetate and crotonyl butyrate were dissolved in vegetable oil according to their respective weight percentages, loaded into the polyethylene bottle, and directly applied after sealing. The field trial of the ratio and dosage of sex attractants for the micro-blight bug was carried out at the Xinjiang Korla Experimental Base of the Institute of Plant Protection, Chinese Academy of Agricultural Sciences from August 3 to August 30, 2024.

[0013] A continuous cotton field with uniform growth and consistent management was selected as the experimental area. A triangular trap was selected as the trap, and an empty polyethylene bottle without any agent was used as the control. The prepared lure was glued to the sticky board and placed in the trap. Each lure was used as a treatment, and 3 replicates were set for each treatment. The traps were randomly arranged with a spacing of no less than 15 meters. The number of adults of the trapped micro-blight bugs was investigated and recorded on the 1st, 3rd, and 5th day after the release of the lure. The results are shown in Tables 1 and 2.

[0014] Table 1 Screening of the ratio of sexual attractants for Microhylidae heteropalpus

[0015]

[0016]

[0017] Note: The data in the table are mean ± standard error; different letters after the data in the same column indicate significant differences at the 0.05 level (Duncan's multiple comparisons), and the same letter indicates no significant difference in the number of traps.

[0018] Table 2 Screening of sexual attractant dosages for Microhylidae heteropalpis

[0019]

[0020] Note: The data in the table are mean ± standard error; different letters after the data in the same column indicate significant differences at the 0.05 level (Duncan's multiple comparisons), and the same letter indicates no significant difference in the number of traps.

[0021] As can be seen from Table 1, single ingredients of hexyl acetate or crotonyl butyrate cannot attract the different-palpiped microsporidia; compared with the control, the combination of hexyl acetate and crotonyl butyrate has a strong attraction to the different-palpiped microsporidia; and the luring effect is best when the ratio of the two is 1:1, and the capture amount can reach 54.40±6.66 heads / trap / day.

[0022] After the ratio screening test, the ratio of hexyl acetate and crotonyl butyrate was determined to be 1:1. Further, a dosage screening test was carried out. As shown in Table 2, a trace amount of components (0.313 mg / lure) can attract the different-palp micro-blight bugs; with the increase of the dose, the number of different-palp micro-blight bugs attracted also increases, but the larger the dose, the better; when the content of the active ingredient is less than 5 mg, the attracting effect decreases. Therefore, the recommended dose of hexyl acetate and crotonyl butyrate in the different-palp micro-blight bug sex attractant is not less than 5 mg / lure. Increasing the content of the active ingredient can also extend the field duration of the lure.

[0023] Five cotton fields with uniform growth were selected as experimental areas to compare the above trap method with the traditional pot-patting method to monitor the effect of the micro-blight bug. The trap method used hexyl acetate (10 mg) and crotonyl butyrate (10 mg) to make the bait core. On the 1st, 4th and 7th day after the bait core was released, the number of micro-blight bug adults falling into the pot and the trap was recorded in the same plot. The results are shown in Table 3.

[0024] Table 3 Comparison of the luring effect of the trap method and the pot-pat method on the microspiny bug

[0025]

[0026] Note: The data in the table are mean ± standard error.

[0027] As shown in Table 3, the number of P. heteropalpis lured by the traps in the five plots was much greater than that of the pot-shooting method. At the same time, the number of P. heteropalpis in the traps can more accurately reflect the population density in the field. Therefore, the trap method described in the present invention can better monitor the population distribution of P. heteropalpis.

[0028] In summary, the sex attractant for Microptera heteropalpaeidae provided by the present invention has the advantages of simple preparation, small dosage, and convenient use. The sex attractant can be used to predict and forecast the population dynamics of Microptera heteropalpaeidae, the natural enemy of pests such as cotton bollworm and thrips, and adjust the application period of chemical pesticides, which is of great significance for protecting natural enemies and comprehensively controlling cotton field pests.

[0029] Finally, it should be noted that the above embodiments are only used to illustrate the technical solution of the present invention rather than to limit it. Although the present invention has been described in detail with reference to the preferred embodiments, those skilled in the art should understand that they can still modify or replace the technical solution of the present invention with equivalents, and these modifications or equivalent replacements cannot cause the modified technical solution to deviate from the spirit and scope of the technical solution of the present invention.

Claims

1. A sex attractant for Lysimachia heteropalpis, characterized in that: The sex attractant component consists of hexyl acetate and crotonyl butyrate, wherein the mass ratio of hexyl acetate to crotonyl butyrate is 1:

1.

2. The method for using the sex attractant of Microhylidae heteropalpis as claimed in claim 1, characterized in that: Hexyl acetate and crotonyl butyrate are mixed according to their respective weight percentages to prepare a sex attractant formula, and then the sex attractant is added into a slow-release carrier polyethylene bottle, and the content of active ingredients in each attractant core is not less than 5 mg.

3. The sex attractant for Microhylidae as claimed in claim 1 needs to be used in combination with a trap, and the trap can be a common pest trapping device such as a triangular trap, a barrel trap, or a boat trap.

4. The sex attractant for Micropygmy Lygus unipalpus as claimed in claim 1 is mainly used for population dynamic monitoring and field spatial distribution regulation of Micropygmy Lygus unipalpus, an important natural enemy of cotton fields in Xinjiang.

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