Method for improving cold resistance and yield of cistanche deserticola y. bailey under chinese tamarix forest in the yellow river delta region

Abstract

The present application belongs to the field of under-forest planting, and particularly relates to a method for improving cold resistance and yield of Cistanche tubulata under Chinese tamarix forest in the Yellow River Delta region. The method improves host root activity and resistance through root system root cutting treatment and spraying treatment liquid; promotes Cistanche tubulata inoculation resistance and survival rate through gibberellin seed dressing, three-layer seeding and spraying Hoagland nutrient solution; enriches soil nutrition, improves soil heat preservation performance, and improves the cold resistance and yield of Cistanche tubulata through near-natural substrate seed dressing, soil microbial fertilizer, farmyard manure and post-covering treatment and other measures. For Cistanche tubulata broken in the fall and winter, the cold resistance is increased by spraying exogenous calcium. The above measures increase the soil temperature by 2-4 DEG C, the cold resistance of Cistanche tubulata under Chinese tamarix forest is improved by more than 63%, and the yield is improved by more than 40%, which has important significance for the industrialization and large-scale cultivation of Cistanche tubulata under Chinese tamarix forest in the Yellow River Delta region.

Description

Technical Field

[0001] This invention belongs to the field of understory planting, specifically relating to a method for improving the cold resistance and yield of Cistanche tubulosa under Tamarix chinensis forests in the Yellow River Delta region. Background Technology

[0002] The statements herein provide only background information in relation to this invention and do not necessarily constitute prior art.

[0003] *Cistanche tubulosa* is a perennial parasitic herb belonging to the genus *Cistanche* in the family Orobanchaceae. It is mainly distributed in the Taklamakan Desert and surrounding areas of southern Xinjiang, often parasitizing the roots of plants in the genus *Tamarix*. The region's arid climate, low rainfall, high evaporation, and long hours of sunshine contribute to *Cistanche tubulosa*'s strong drought tolerance, but its cold resistance is relatively poor.

[0004] *Tamarix chinensis* Lour., a deciduous shrub or small tree belonging to the genus *Tamarix* in the family Tamarixaceae, is a major woody plant species in saline-alkali areas. It possesses physiological characteristics such as salt and alkali tolerance, drought tolerance, and tolerance to poor soil, and has become a primary ecological restoration tree species in the Yellow River Delta saline-alkali region. Currently, research on inoculating *Cistanche tubulosa* with *Tamarix* has been carried out in the Yellow River Delta region. Among these studies, Yang Qingshan et al. explored environmental factors affecting *Cistanche tubulosa* yield, including soil moisture and temperature, as well as practical factors (cultivation depth, host root distribution), clarifying the influencing factors. Hou Lei et al. investigated the inoculation rate and growth status of *Cistanche tubulosa* in the Yellow River Delta, concluding that it is feasible to utilize wild *Tamarix* for inoculation and production of *Cistanche tubulosa* in the saline-alkali land of the Yellow River Delta.

[0005] The Yellow River Delta region, as a transitional zone between terrestrial and marine ecosystems, possesses a unique ecological type. The region suffers from severe soil salinization, a low water table, and cold, dry winters with frequent strong winds. Studies have shown that *Cistanche tubulosa* has a water content exceeding 80%, with a critical cold-resistance temperature of -2.5℃ to -3℃. However, the lowest winter temperatures in the Yellow River Delta region are mostly around -5℃ to -10℃, and soil temperatures are mostly between -2℃ and -6℃, exhibiting prominent characteristics such as high soil moisture, prolonged periods of low temperatures, and deep permafrost. Therefore, frost damage is identified as a major factor affecting the cultivation and yield of *Cistanche tubulosa* in the Yellow River Delta region. Furthermore, the harvest time for *Cistanche tubulosa* in this region is approximately from late April to mid-May. Therefore, improving the cold resistance of *Cistanche tubulosa* itself and enhancing soil insulation are of great significance for its cultivation.

[0006] While there are some research reports on the cultivation of Cistanche tubulosa under Tamarix chinensis forests in the Yellow River Delta, these reports mainly focus on conventional cultivation methods. However, no research has been reported on improving the cold resistance of Cistanche tubulosa under Tamarix chinensis forests in this region. Summary of the Invention

[0007] To overcome the shortcomings of the existing technology, this invention provides a method for improving the cold resistance and yield of *Cistanche tubulosa* under *Tamarix chinensis* forests in the Yellow River Delta region. Addressing the prominent problems of poor cold resistance and low yield of *Cistanche tubulosa*, this invention improves the root vitality of the host plant, *Tamarix chinensis*, through various measures such as root treatment, seed treatment, sowing treatment, soil treatment, mulching treatment, and cultivation management. This promotes the inoculation rate, yield, and cold resistance of *Cistanche tubulosa*, and is of great significance for the industrialization and large-scale development of *Cistanche tubulosa* in the Yellow River Delta region.

[0008] To achieve the above objectives, the present invention employs the following technical solutions:

[0009] A method for improving the cold resistance and yield of *Cistanche tubulosa* under *Tamarix chinensis* forests in the Yellow River Delta region includes root treatment, seed treatment, sowing depth selection, and soil treatment.

[0010] The root treatment method is as follows: one year before inoculating Cistanche tubulosa, using the trunk as a reference, cut the lateral roots and fibrous roots vertically downwards for 40-50cm at a distance of 35-40cm on both sides of the row, and spray the cut roots with treatment solution at the same time.

[0011] The seed treatment method is as follows: On the day of sowing, soak the seeds of *Cistanche tubulosa* in warm water at 30-35℃ for 5-6 hours, then quickly rinse with 75% alcohol for 1-2 minutes, followed by rinsing with running water 2-3 times. Finally, treat with a solution of 18-20 mg / kg. -1 Seed dressing with gibberellin;

[0012] The sowing treatment method is as follows: Determine the sowing depth. From late April to early June, dig a trench 35-40cm away from the main trunk of Tamarix chinensis, with a width of 25-30cm. Based on the root distribution characteristics of Tamarix chinensis during root treatment, determine the trench depth to be 50-60cm. Sow the seeds using a three-layer sowing method.

[0013] The soil treatment method is as follows: after sowing in the same year, apply 2-3 kg of microbial fertilizer per mu in July and August in conjunction with inter-cultivation and loosening of the soil. The depth of inter-cultivation should not be too shallow, and should be controlled at 15-20 cm. From mid-October to early November, apply an additional 700-800 kg of farmyard manure per mu.

[0014] In some embodiments, the method also includes the step of selecting a Tamarix chinensis plantation. The method of selecting a Tamarix chinensis plantation is as follows: select a Chinese Tamarix chinensis forest with soil texture of light loam or sandy soil, with an annual groundwater level below 1.2m, and the Chinese Tamarix chinensis should be in good growth, have a well-developed root system, be free from pests and diseases, and be 3-5 years old.

[0015] In some embodiments, in the root treatment method, the treatment solution is prepared from glucose, acetic acid and glycine, wherein, based on the weight of water added, the mass percentages of each component are: glucose 45%, acetic acid 15%, and glycine 40%.

[0016] In some embodiments, during the seed treatment method, a substrate is added when gibberellin is used to coat the seeds. Since the seeds of *Cistanche deserticola* are relatively small, the mass ratio of seeds to substrate is 1:4-1:6. The substrate is prepared from peat moss, topsoil from tamarisk forests, perlite, and bagasse, with the mass ratio of peat moss, topsoil from tamarisk forests, perlite, and bagasse being 4:3:2:1.

[0017] In some embodiments, the three-layer sowing method specifically involves: sowing the seeds mixed with substrate at the bottom of the furrow in a single layer, filling the furrow with 10cm of soil, and then sowing another layer, repeating this process three times. Each layer is sprayed with a 1 / 2 concentration of modified Hoagland nutrient solution. After the third layer is sown, a 2-3cm layer of substrate is sown on top. The substrate is composed of peat moss, tamarisk forest topsoil humus, perlite, and bagasse, wherein the mass ratio of peat moss, tamarisk forest topsoil humus, perlite, and bagasse is 4:3:2:1.

[0018] Preferably, the sowing rate of Cistanche tubulosa is controlled at 260–300 g / 666.7 m². 2 .

[0019] In some embodiments, a mulching treatment step is also included, wherein the mulching treatment method is as follows: in late November of the year of sowing, black weed control cloth is used to cover the ground surface, centered on the main trunk of the Chinese tamarisk and extending outward to near the drip line of the canopy, and the ground cover is removed in late March of the following year.

[0020] In some embodiments, the process also includes steps of irrigation, loosening the soil, weeding, pruning, and pest and disease control. In the pruning process, in accordance with the principles of conventional branch pruning, the branches between rows are heavily shortened by 1 / 2 to 3 / 4.

[0021] In some embodiments, for plots near the coast or windy areas, windbreaks should be built around or within the Chinese tamarisk forest during winter. The windbreaks should be made of straw or straw mats, with a height of 100-120cm and a depth of 30cm.

[0022] In some embodiments, for Cistanche tubulosa that sprouts in autumn and winter of the same year, exogenous calcium is also sprayed onto its surface.

[0023] In some embodiments, for *Cistanche tubulosa* that sprouts in autumn and winter of the same year, 15.0 mmol·L⁻¹ is applied twice. -1 Spray the newly emerged Cistanche tubulosa with a CaCl2 solution of a certain concentration, with an interval of 10-15 days between two applications.

[0024] The above one or more technical solutions have the following beneficial effects:

[0025] This invention addresses the prominent problems of poor cold resistance and low yield of *Cistanche tubulosa* under *Tamarix chinensis* forests in the Yellow River Delta region, proposing a method to improve the yield and cold resistance of *Cistanche tubulosa* under *Tamarix chinensis* forests in the Yellow River Delta region. This method improves the host root vigor and resistance through root pruning and spraying with a treatment solution; promotes inoculation resistance and survival rate of *Cistanche tubulosa* through gibberellin seed treatment, three-layer sowing, and spraying with Hoagland nutrient solution; enriches soil nutrients and improves soil insulation performance through near-natural substrate seed treatment, soil enrichment with microbial fertilizer and farmyard manure, and post-planting mulching, thereby improving the cold resistance and yield of *Cistanche tubulosa*. Furthermore, for *Cistanche tubulosa* that sprouts in autumn and winter, exogenous calcium is sprayed to increase its cold resistance. Through the above measures, the soil temperature can be increased by 2-4℃, the cold resistance of Cistanche tubulosa under Tamarix chinensis forests can be improved by more than 63%, and the yield can be increased by more than 40%. This is of great significance for the industrialization and large-scale cultivation of Cistanche tubulosa under Tamarix chinensis forests in the Yellow River Delta region. Attached Figure Description

[0026] The accompanying drawings, which form part of this invention, are used to provide a further understanding of the invention. The illustrative embodiments of the invention and their descriptions are used to explain the invention and do not constitute an improper limitation of the invention.

[0027] Figure 1 This refers to the root interruption and spraying treatment solution in Example 1 of the present invention;

[0028] Figure 2 This describes the inoculation of Cistanche tubulosa onto a single Tamarix chinensis plant in Example 1 of the present invention;

[0029] Figure 3 This is a description of the emergence of Cistanche tubulosa from Tamarix chinensis forests in China in April after overwintering, according to Example 1 of the present invention. Detailed Implementation

[0030] It should be noted that the following detailed descriptions are illustrative and intended to provide further explanation of this application. Unless otherwise specified, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application pertains.

[0031] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the exemplary embodiments according to this application. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0032] Example 1:

[0033] Cistanche tubulosa was inoculated under Tamarix chinensis forests in China. The experiment was divided into two groups, with each group having an inoculation length of 10m and a seed amount of 16.2g. The first group was the experimental group, and the second group was the control group. The seeds of Cistanche tubulosa were sourced from Hotan Prefecture, Xinjiang Uygur Autonomous Region.

[0034] The experimental group underwent cold resistance treatment using the following methods:

[0035] A method for improving the cold resistance and yield of Cistanche tubulosa under Tamarix chinensis forests in the Yellow River Delta region includes the following steps:

[0036] (1) Selection of Tamarix chinensis plantations: Select Tamarix chinensis forest land with light loam or sandy soil texture, and the groundwater level should be below 1.2m all year round. Tamarix chinensis should be in good growth, have well-developed root system, and be free from diseases and pests. The forest age should be 3-5 years. The experimental forest in this example is a 4-year-old 'Lu Ting No. 1' Tamarix chinensis forest with a plant spacing of 3m×2m.

[0037] (2) Root treatment: One year before inoculating *Cistanche tubulosa*, using the trunk as a reference, cut the lateral roots and fibrous roots vertically downwards for 40-50 cm, 35-40 cm from the ground on both sides of the tree, perpendicular to the ground. The cuts should be smooth to facilitate wound healing and fine root growth. Simultaneously, spray the cut roots with a treatment solution. Figure 1 The treatment solution is prepared from glucose, acetic acid, and glycine. Based on the weight of water added, the mass percentages of each component are: glucose 45%, acetic acid 15%, and glycine 40%. By increasing the relative content of certain sugars, organic acids, and amino acids, the concentration and exudation capacity of root exudates are enhanced, thereby promoting fine root growth and improving stress resistance.

[0038] (3) Seed treatment: On the day of sowing, soak the seeds of Cistanche tubulosa in warm water at 30-35℃ for 5-6 hours, then rinse them quickly with 75% alcohol for 1-2 minutes, followed by rinsing with running water 2-3 times. Finally, treat with a solution of 18-20 mg / kg. -1Gibberellin is used for seed dressing to promote the germination and haustorium formation of Cistanche tubulosa seeds, increasing inoculation success rate and stress resistance. Because the thousand-seed weight of Cistanche tubulosa is relatively small, a substrate can be added during gibberellin seed dressing. The seed-to-substrate mass ratio is 1:4 to facilitate subsequent sowing. The substrate is prepared from peat moss, topsoil from tamarisk forest, perlite, and bagasse, with a mass ratio of 4:3:2:1.

[0039] (4) Sowing treatment: From late April to early June, dig a trench 35-40cm away from the main trunk of Chinese tamarisk, with a width of 25-30cm. Based on the root distribution characteristics of Chinese tamarisk during the root treatment process, determine the trench depth to be 50-60cm. The seeds, after being mixed with the substrate, were then sown in a single layer at the bottom of the furrow. After filling the furrow with 10cm of soil, another layer was sown, for a total of three layers. Each layer was sprayed with a 1 / 2 concentration of modified Hoagland nutrient solution to supplement nutrients from the outside, improving seed resistance and promoting survival rate and yield in later inoculation. After the third layer was sown, a 2-3cm layer of substrate was spread on top. This substrate consisted of peat moss, topsoil from tamarisk forest, perlite, and bagasse, with a mass ratio of 4:3:2:1. This near-natural substrate helped to induce seed germination and improve seedling cold resistance and soil insulation after inoculation. The sowing rate of *Cistanche tubulosa* was controlled at 260-300g / 666.7m². 2 Finally, the trench was backfilled and compacted.

[0040] (5) Soil treatment: After sowing in the same year, apply 2-3 kg of microbial fertilizer per mu (667 square meters) in July and August, combined with inter-cultivation and loosening of the soil. The cultivation depth should not be too shallow, controlled at 15-20 cm. The application of microbial fertilizer promotes the absorption of soil nutrients by the roots of *Tamarix chinensis* and the secretion of key parasitic substances, improves root vitality and stress resistance, thereby improving the growth and cold resistance of *Cistanche tubulosa*. The microbial fertilizer contains ≥50% organic matter, ≥20% humus, ≥4% N+P2O5+K2O, ≥200 million / g total viable bacteria, and ≥50 million / g effective viable bacteria. Subsequently, from mid-October to early November, apply 700-800 kg of farmyard manure per mu, which can increase the soil's heat absorption and water retention capacity. Under the action of microbial fertilizer, the organic matter in the farmyard manure decomposes rapidly in winter, continuously releasing heat energy, thereby increasing the soil temperature and providing nutrients for the later growth of *Cistanche tubulosa*.

[0041] (6) Mulching: In late November of the year of sowing, mulching can be carried out from the main trunk of the Chinese tamarisk tree outwards to the vicinity of the canopy drip line to increase soil temperature. Black weed control cloth should be used for mulching, which can form an insulating layer by absorbing solar and soil heat radiation, thereby reducing soil evaporation and heat dissipation. In addition, the black weed control cloth should be removed in time in late March of the following year to avoid affecting the emergence and growth of Cistanche tubulosa.

[0042] (7) Nurturing and management measures: Based on the growth characteristics of Chinese tamarisk and Cistanche tubulosa under the forest, regular irrigation, loosening of soil, weeding, pruning and pest and disease control work shall be carried out.

[0043] To reduce the risk of frost damage, irrigation should be prohibited after the end of November to prevent reduced soil aeration, which would affect the respiration of the *Tamarix chinensis* roots and *Cistanche tubulosa*. Furthermore, during pruning, in addition to following regular branch pruning principles, inter-row branches can be heavily shortened by 1 / 2 to 3 / 4 to increase light penetration, thereby raising soil temperature and facilitating later harvesting. In winter, for plots near the coast or windy areas, windbreaks should be erected around or within the *Tamarix chinensis* forest to reduce the impact of cold winds on the *Cistanche tubulosa* under the forest canopy. Windbreaks can be made of straw or straw mats, with a height of 100-120 cm and a 30 cm burial depth. Simultaneously, for *Cistanche tubulosa* that emerges from the soil in autumn and winter, in addition to the above-mentioned nurturing management, apply 15.0 mmol·L⁻¹ of [treatment method missing] twice. -1 Spray the newly emerged Cistanche tubulosa with a CaCl2 solution of a certain concentration, with an interval of 10-15 days between two applications.

[0044] The control group was treated using the conventional method of inoculating Cistanche tubulosa.

[0045] (1) Selection of Tamarix chinensis plantations: Select Tamarix chinensis forest land with light loam or sandy soil texture, and the groundwater level should be below 1.2m all year round. Tamarix chinensis should be in good growth, have well-developed root system, and be free from diseases and pests. The forest age should be 3-5 years. The experimental forest in this example is a 4-year-old 'Lu Ting No. 1' Tamarix chinensis forest with a plant spacing of 3m×2m.

[0046] (2) Root treatment: No root pruning treatment was performed.

[0047] (3) Seed treatment: On the day of sowing, Cistanche tubulosa seeds were soaked in warm water at 30-35℃ for 5-6 hours, then rinsed quickly with 75% alcohol for 1-2 minutes, and then rinsed with running water 2-3 times. No gibberellin treatment was performed. The seeds were directly mixed with the substrate at a ratio of 1:4. The substrate was river sand.

[0048] (4) Sowing treatment: From late April to early June, dig trenches 35-40 cm away from the main trunk of *Tamarix chinensis*, with a width of 25-30 cm. Based on the root distribution characteristics of *Tamarix chinensis*, the trench depth should be 50 cm. The sowing rate of *Cistanche tubulosa* should be controlled at 260-300 g / 666.7 m². 2 Finally, the trench was backfilled and compacted.

[0049] (5) Soil treatment: After sowing in the same year, apply 2-3 kg of ordinary compound fertilizer per mu in July and August in conjunction with inter-cultivation and loosening of the soil. The depth of inter-cultivation should not be too shallow, and should be controlled at 15-20 cm. After that, from mid-October to early November, apply 700-800 kg of farmyard manure per mu to increase the soil's heat absorption and water retention capacity.

[0050] (6) Covering treatment: In late November of the year of sowing, the ground can be covered with straw to increase soil temperature, with the main trunk of Chinese tamarisk as the center and extending outward to the vicinity of the canopy drip line.

[0051] (7) Nurturing and management measures: Based on the growth characteristics of Chinese tamarisk and Cistanche tubulosa under the forest, regular irrigation, loosening of soil, weeding, pruning and pest and disease control are carried out.

[0052] Among them, the inoculation of Cistanche tubulosa onto individual plants of Tamarix chinensis in the experimental group is as follows: Figure 2 As shown, the emergence of Cistanche tubulosa from Tamarix chinensis forests in China in April after overwintering is as follows: Figure 3 As shown.

[0053] The method of this invention and conventional methods were used to determine the number of *Cistanche tubulosa* plants emerging from the soil (measured from a 5-meter depth) one year after inoculation in *Tamarix chinensis* forests in China, as well as the yield and soil temperature at a depth of 40 cm in January. The median lethal temperature was calculated using electrical conductivity.

[0054] Following the method described in this application, 46 *Cistanche tubulosa* plants emerged one year after inoculation, yielding 1109.3 kg per mu (approximately 0.067 hectares) with a soil temperature of 1.2℃, as shown in Table 1.

[0055] Table 1

[0056]

[0057] Example 2:

[0058] The experimental forest consisted of 4-year-old 'Lu Cheng No. 1' Chinese tamarisk forest, with a plant spacing of 3m × 2m.

[0059] Experimental methods: The root pruning + spraying treatment, gibberellin seed dressing, and three-layer sowing + Hoagland nutrient solution treatments were used individually in the experimental group of Example 1. Other treatment steps were conventional. The untreated (CK) group followed the same method as the control group in Example 1 (conventional method). The inoculation length, seed quantity, and seed source of *Cistanche tubulosa* for the different treatment groups were the same as in Example 1.

[0060] The number of *Cistanche tubulosa* plants emerging from the soil (measured at a depth of 5 meters) one year after inoculation in *Tamarix chinensis* forests in China, the yield, and the soil temperature at a depth of 40 cm in January were measured.

[0061] As shown in Table 2, using only root pruning and spraying treatment, 30 *Cistanche tubulosa* plants emerged, with a yield of 647.2 kg per mu; using only gibberellin seed treatment, 32 *Cistanche tubulosa* plants emerged, with a yield of 716.5 kg per mu; using only three-layer sowing and Hoagland nutrient solution treatment, 35 *Cistanche tubulosa* plants emerged, with a yield of 802.4 kg per mu.

[0062] Table 2

[0063]

[0064] Example 3:

[0065] The experimental forest consisted of 4-year-old 'Lu Cheng No. 1' Chinese tamarisk forest, with a plant spacing of 3m × 2m.

[0066] Experimental methods: Different sowing and soil treatments were selected, and the following methods were used individually: post-sowing substrate preparation, application of microbial fertilizer alone, application of farmyard manure alone, and application of microbial fertilizer + farmyard manure. Other treatment steps were the same as conventional treatment methods. *Cistanche tubulosa* was inoculated. The untreated (CK) group followed the same method as the control group in Example 1 (conventional method). The inoculation length, seed quantity, and seed source of *Cistanche tubulosa* for the different treatment groups were the same as in Example 1.

[0067] The number of *Cistanche tubulosa* plants emerging from the soil (measured at a depth of 5 meters) one year after inoculation in *Tamarix chinensis* forests in China, the yield, and the soil temperature at a depth of 40 cm in January were measured.

[0068] As shown in Table 3, using the single method described above, with substrate laid after sowing, 32 *Cistanche tubulosa* plants emerged, yielding 688.7 kg per mu (approximately 0.067 hectares), and the soil temperature reached 0.2℃; with microbial fertilizer applied alone, 29 *Cistanche tubulosa* plants emerged, yielding 620.4 kg per mu (approximately 0.067 hectares), and the soil temperature reached -0.6℃; with farmyard manure applied alone, 26 *Cistanche tubulosa* plants emerged, yielding 594.2 kg per mu (approximately 0.067 hectares), and the soil temperature reached -0.3℃; with a combination of microbial fertilizer and farmyard manure, 31 *Cistanche tubulosa* plants emerged, yielding 769.5 kg per mu (approximately 0.067 hectares), and the soil temperature reached 0.5℃.

[0069] Table 3

[0070]

[0071] Example 4:

[0072] The experimental forest consisted of 4-year-old 'Lu Cheng No. 1' Chinese tamarisk forest, with a plant spacing of 3m × 2m.

[0073] Experimental methods: Different covering treatments were selected, using the black weed control cloth and soil covering methods from the experimental group in Example 1, while other treatment steps were performed using conventional methods. *Cistanche tubulosa* was inoculated. The untreated (CK) group followed the same method as the control group in Example 1 (conventional method). The inoculation length, seed quantity, and seed source of *Cistanche tubulosa* for the different treatment groups were the same as in Example 1.

[0074] The number of *Cistanche tubulosa* plants emerging from the soil (measured at a depth of 5 meters) one year after inoculation in *Tamarix chinensis* forests in China, the yield, and the soil temperature at a depth of 40 cm in January were measured.

[0075] As shown in Table 4, based on the single method described above, using black weed control cloth covering resulted in 27 Cistanche tubulosa plants emerging, with a yield of 581.2 kg per mu and a soil temperature of 1.2℃; using earthing up covered soil resulted in 24 Cistanche tubulosa plants emerging, with a yield of 466.4 kg per mu and a soil temperature of -0.7℃.

[0076] Table 4

[0077]

[0078] Example 5:

[0079] The experimental forest consisted of 4-year-old 'Lu Cheng No. 1' Chinese tamarisk forest, with a plant spacing of 3m × 2m.

[0080] Experimental methods: Different cultivation and management measures were selected, and the winter irrigation, pruning, and windbreak construction methods of the experimental group in Example 1 were used separately. Other treatment steps were the conventional treatment methods. Cistanche tubulosa was inoculated. The untreated (CK) group was treated in the same way as the control group in Example 1 (conventional method). Among them, the inoculation length, seed amount, and seed source of Cistanche tubulosa in the above different treatment groups were the same as in Example 1.

[0081] The number of *Cistanche tubulosa* plants emerging from the soil (measured at a depth of 5 meters) one year after inoculation in *Tamarix chinensis* forests in China, the yield, and the soil temperature at a depth of 40 cm in January were measured.

[0082] As shown in Table 5, according to a method described in this patent application, using winter irrigation, 8 *Cistanche tubulosa* plants emerged, yielding 194.3 kg per mu, and the soil temperature reached -4.2℃; using pruning, 20 *Cistanche tubulosa* plants emerged, yielding 401.6 g per mu, and the soil temperature reached -2.4℃; using windbreaks, 21 *Cistanche tubulosa* plants emerged, yielding 324.4 g per mu, and the soil temperature reached -1.9℃.

[0083] Table 5

[0084]

[0085] The above description is merely a preferred embodiment of the present invention and is not intended to limit the invention. Various modifications and variations can be made to the present invention by those skilled in the art. Any modifications, equivalent substitutions, improvements, etc., made within the spirit and principles of the present invention should be included within the scope of protection of the present invention.

Claims

1. A method for improving the cold resistance and yield of Cistanche tubulosa under the Chinese tamarix forest in the Yellow River Delta region, characterized in that, This includes root treatment, seed treatment, sowing depth selection, and soil treatment, among which... The root treatment method is as follows: one year before inoculating Cistanche tubulosa, using the trunk of Tamarix chinensis as a reference, cut the lateral roots and fibrous roots vertically downwards for 40-50cm at 35-40cm on both sides of the row, and spray the cut roots with treatment solution at the same time. The method of seed treatment is that on the day of sowing, the Cistanche tubulosa seeds are placed in warm water at 30-35°C, soaked for 5-6 hours, rinsed with 75% alcohol for 1-2 minutes, washed with running water for 2-3 times, and then treated with gibberellin at a concentration of 18-20 mg·kg -1 of seeds. The sowing method is as follows: from late April to early June, dig a trench 35-40 cm away from the main trunk of the Chinese tamarisk, with a width of 25-30 cm and a depth of 50-60 cm; sow the seeds in three layers. The soil treatment method is as follows: after sowing in the same year, in July and August, apply 2-3 kg of microbial fertilizer per mu in conjunction with inter-cultivation and loosening of the soil, and control the inter-cultivation depth to 15-20 cm; from mid-October to early November, apply 700-800 kg of farmyard manure per mu. In the root treatment method, the treatment solution is prepared from glucose, acetic acid and glycine; In the seed treatment method, a substrate is added when gibberellin is used for seed dressing. The substrate is made of peat moss, tamarisk forest topsoil, perlite and bagasse, wherein the mass ratio of peat moss, tamarisk forest topsoil, perlite and bagasse is 4:3:2:

1. For the Cistanche tubulosa that sprouts in autumn and winter of that year, it also includes spraying exogenous calcium on its surface; Microbial fertilizer contains ≥50% organic matter, ≥20% humus, ≥4% N+P2O5+K2O, ≥200 million live bacteria / g, and ≥50 million effective live bacteria / g. In the sowing treatment method, the three-layer sowing method is as follows: after mixing the seeds with the substrate, sow a layer at the bottom of the furrow, fill the soil with 10cm, and then sow another layer. Sow a total of three layers upwards. Spray each layer with 1 / 2 concentration of modified Hoagland nutrient solution. After the third layer is sown, sow a 2-3cm layer of substrate on the top layer. The Cistanche tubulosa seed amount is controlled at 260-300 g / 666.7 m 2 .

2. The method of claim 1, wherein, It also includes the steps for selecting tamarisk plantations. The method for selecting tamarisk plantations is as follows: select Chinese tamarisk forest land with soil texture of light loam or sandy soil, groundwater level below 1.2m, and forest age of 3-5 years.

3. The method of claim 1, wherein, It also includes a mulching treatment step, the method of which is as follows: in late November of the year of sowing, black weed control cloth is used to cover the ground surface, centered on the main trunk of the Chinese tamarisk and extending outward to the drip line of the canopy, and the ground cover is removed in late March of the following year.

4. The method of claim 1, wherein, It also includes steps such as irrigation, loosening the soil, weeding, pruning, and pest and disease control. Among them, pruning follows the conventional pruning principle, and the branches between rows are heavily shortened by 1 / 2 to 3 / 4.

5. The method of claim 1, wherein, For plots near the coast or windy areas, windbreaks should be built around or inside the Chinese tamarisk forests during winter. The windbreaks should be made of straw or straw mats, with a height of 100-120cm and buried 30cm deep.

6. The method of claim 1, wherein, For the Cistanche tubulosa broken soil in autumn and winter, 15.0 mmol·L -1 -15 days interval.

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