Artificial planting management method for cyanotis arachnoidea

Abstract

The invention provides a cyanotis arachnoidea artificial planting management method, and relates to the technical field of cyanotis arachnoidea planting. The artificial planting management method for the cyanotis arachnoidea mainly comprises the steps that cyanotis arachnoidea seeds are disinfected and then fumigated with alpha-terpilenol; soaking pea vines in saline water, grinding, filtering and drying to prepare a pea vine extract, and treating the cyanotis arachnoidea by the pea vine extract and other fertilizers in the sowing and seedling emergence period and the transplanting period of the cyanotis arachnoidea. The defects in the prior art are overcome, the growth efficiency of early seedlings of the cyanotis arachnoidea is effectively improved, growth expansion of roots of the cyanotis arachnoidea and accumulation of ecdysone are further promoted, and the yield and quality of artificial planting of the cyanotis arachnoidea are comprehensively improved.

Description

Technical Field

[0001] The invention relates to the technical field of dew grass planting, and in particular to a method for artificial planting and management of dew grass. Background Art

[0002] Dew grass, also known as pearl dew grass, lung-changing powder, and cockscomb ginseng, is a perennial herb of the genus Blue Ear Grass of the Commelinaceae family. In my country, it mainly grows in ravines, hillsides, forest edges or forests at an altitude of 1100-2700m in Yunnan, Guizhou, Guangxi, Guangdong, Fujian and other places. Its main effective part is the root, which contains a molting hormone that promotes cell growth, stimulates dermal cell division, and promotes human protein synthesis. It has good prospects in the field of medicine.

[0003] Due to the high overall survival rate of dew grass, most of the existing dew grass planting is relatively extensive, usually directly sowing in the field. There are few detailed studies on the actual planting and management of dew grass, which can easily lead to the final yield and quality of artificially cultivated dew grass being relatively uneven, affecting the economic benefits of actual planting. Summary of the invention

[0004] In view of the shortcomings of the existing technology, the present invention provides a method for artificial planting and management of dew grass, which effectively promotes the growth of dew grass roots and further enhances the accumulation of root ecdysone, thereby comprehensively improving the yield and economic benefits of artificial planting of dew grass.

[0005] To achieve the above objectives, the technical solution of the present invention is implemented through the following technical solutions:

[0006] A method for artificial planting and management of dew grass, the method comprising the following steps:

[0007] S1. Seed pretreatment: After sterilizing mature dew grass seeds, fumigate them in an environment with an α-terpineol content of 0.2-0.5 g / L for 1-2 hours to obtain pretreated seeds for later use;

[0008] S2. Seed sowing treatment: sterilize the planting soil and add basal fertilizer, then sow the pretreated seeds and irrigate them with clean water;

[0009] S3, preparation of pea vine extract: cut pea vine into sections, mix with 1%-1.5% salt water, treat at 50-60°C for 15-20 minutes, filter, add clean water to the pea vine, grind and filter, and obtain pea vine extract;

[0010] S4. Seedling management: After sowing and germination, water thoroughly once with a fertilizer containing 100-200 mg / L pea vine extract and 10-15 g / L urea;

[0011] S5. Transplantation management: fertilize the transplanted field and then plow it, then transplant the dew grass seedlings that have grown to 5-6 leaves into the field, and use 100-200 mg / L pea vine extract as rooting water to irrigate thoroughly;

[0012] S6. Fertilization management: conventional fertilization treatment, and supplementary watering with pea vine extract at a concentration of 100-200 mg / L 2-3 months after transplanting.

[0013] Preferably, the method of disinfecting the dew grass seeds in step S1 is to first air-dry them for 2-3 days, and then soak them in 3000-5000 times potassium permanganate solution or 1000-5000 times thiabendazim solution for 5-8 hours.

[0014] Preferably, in step S2, the planting soil is selected from at least one of ordinary garden soil, red soil, brown soil or sandy loam, and the planting soil is disinfected by spraying a 0.05%-0.1% potassium permanganate solution, then covered with a film and allowed to stand for 1-2 days before being removed and naturally dried for 5-7 days.

[0015] Preferably, the base fertilizer in step S2 is manure, and the mixing ratio of planting soil and base fertilizer is 4-6:1.

[0016] Preferably, in step S3, the mass ratio of adding pea vine to clean water for grinding and filtering is 1:3, and the grinding and filtering method is grinding at a speed of 12000-18000 r / min for 5-10 min.

[0017] Preferably, the temperature of the seeds during sowing and germination is controlled at 12-20°C.

[0018] Preferably, the timing of using water and fertilizer in step S4 is after the germination rate reaches more than 50%.

[0019] Preferably, the amount of field fertilization in step S5 is 1200-1500 kg / mu, and the fertilizer for field fertilization is a mixture of manure, superphosphate, wood ash and potassium sulfate in a mass ratio of 100-120: 0.3-0.5: 0.4-0.6: 0.1-0.3.

[0020] Preferably, the conventional fertilization treatment in step S6 is to use manure water + 10-15g / L urea as fertilizer at one month and three months after transplanting.

[0021] The present invention provides a method for artificial planting and management of dew grass, which has the following advantages compared with the prior art:

[0022] The present invention effectively improves the growth efficiency of early dew grass seedlings through early α-pineneol fumigation of seeds and subsequent auxiliary treatment of pea vine extract on dew grass seedling emergence and transplanting, and further promotes the growth and expansion of dew grass roots and the accumulation of ecdysone, comprehensively improves the yield and quality of artificially planted dew grass, increases the economic benefits of planting, is suitable for large-scale artificial planting, and ensures the overall planting quality. BRIEF DESCRIPTION OF THE DRAWINGS

[0023] Figure 1 This is a schematic diagram of the planting soil filled in the seedling tray in an embodiment of the present invention;

[0024] Figure 2 This is a schematic diagram of the growth of dew grass in each experimental group 70 days after sowing in the embodiment of the present invention;

[0025] Figure 3 This is a schematic diagram of flowering of the dew grass after transplanting in the experimental group 1 of the embodiment of the present invention;

[0026] Figure 4 This is a schematic diagram of the comparison of the roots of the experimental groups 1-3 of the dew grass after harvest in the embodiment of the present invention;

[0027] Figure 5 This is a schematic diagram of the comparison of the roots of the experimental groups 4-6 of dew grass after harvest in the embodiment of the present invention;

[0028] Figure 6 This is a schematic diagram comparing the roots of the harvested dew grass in the experimental groups 7-8 in the examples of the present invention. DETAILED DESCRIPTION

[0029] In order to make the purpose, technical solution and advantages of the embodiments of the present invention clearer, the technical solution in the embodiments of the present invention is clearly and completely described below in combination with the embodiments of the present invention. Obviously, the described embodiments are part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without creative work are within the scope of protection of the present invention.

[0030] Example:

[0031] Experiment on artificial planting of dew grass:

[0032] 1. Seed pretreatment:

[0033] Select the same batch of mature dew grass seeds, dry them for 3 days, and then soak them in 5000 times dilution of thiophanate-methyl for 8 hours to obtain pretreated seeds SD-1;

[0034] Take some SD-1 seeds and fumigate them in an environment with an α-terpineol content of 0.2 g / L for 2 hours to obtain pretreated seeds SD-2;

[0035] Take some SD-1 seeds and fumigate them in an environment with an α-terpineol content of 0.5 g / L for 1 hour to obtain pretreated seeds SD-3;

[0036] Take some SD-1 seeds and fumigate them in an environment with an α-terpineol content of 1 g / L for 1 hour to obtain pretreated seeds SD-4;

[0037] 2. Preparation of Pisum sativum Extract:

[0038] (1) Cut the pea vine into segments, place them in 1.5% salt water at 60°C for 15 minutes, and then filter them. Add 3 times the weight of clean water to the filter residue, grind them in a grinder at a speed of 15000 r / min for 10 minutes, and then press and filter. The filtrate is dried at 40°C to obtain PE-1.

[0039] (2) adding pea vine to 3 times the mass of clean water and grinding it with a grinder at a speed of 15000 r / min for 10 min, then squeezing and filtering, and drying the filtrate at a constant temperature of 40°C to obtain PE-2;

[0040] 3. Seeding process:

[0041] Ordinary garden soil was selected as planting soil, and potassium permanganate solution with a concentration of 0.08% was sprayed on it, and then covered with a film and allowed to stand for 2 days, then the film was removed and naturally aired for 7 days, and then sheep manure was mixed with it according to a mass ratio of 5:1, and then the planting soil mixed with sheep manure was filled into a 4×8 grid seedling tray (such as Figure 1 As shown), place the seedling tray in the greenhouse and control the temperature of the greenhouse at 12-20℃ (temperature difference ≤5℃ in 24h);

[0042] Sow the pretreated seeds in the seedling tray at a rate of 2-3 seeds per grid, cover with a layer of planting soil, irrigate with water, and irrigate with water and fertilizer after 15 days (the germination rate is above 50%).

[0043] 4. Transplanting management:

[0044] Eight planting areas were set up in Xinping County, Yunnan Province. After tilling, basal fertilizer (sheep manure, superphosphate, plant ash, potassium sulfate mass ratio of 120:0.4:0.5:0.1) was applied at a rate of 1500 kg / mu. Dew grass seedlings (grown to 5-6 leaves) sown for 75 days were transplanted to each planting area on April 10, 2024, and watered thoroughly to establish roots.

[0045] One month after transplanting, the first fertilizer should be applied at 600 kg / mu, and three months after transplanting, the second fertilizer should be applied at 600 kg / mu.

[0046] Referring to the above artificial planting method, experimental groups 1-8 as shown in Table 1 were set up respectively:

[0047] Table 1

[0048]

[0049] The manure water in the above table is sheep manure water with a mass concentration of 15%.

[0050] 5. Experimental results verification:

[0051] (1) The growth of dew grass seedlings in each experimental group after 70 days of sowing was as follows Figure 2 As shown, 10 dew grass seedlings were randomly selected from each group to measure the seedling height (the height of the longest leaf above the ground), and the average value was calculated. The specific results are shown in Table 2 below:

[0052] Table 2

[0053]

[0054] From the above table 2 and Figure 2 It can be seen that the dew grass sown in experimental groups 1, 2, 3, 5, 6 and 7 all had good seedling growth effects, while the seeds in experimental group 4 were not fumigated with α-pineneol and pea vine extract PE-1 was not added to the subsequent water and fertilizer (equivalent to the blank control group), and the overall seedling growth effect was poor, that is, the addition of α-pineneol fumigation and PE-1 can improve the growth of seedlings to a certain extent, while in experimental group 8, PE-2 was added to the water and fertilizer, and the overall seedling growth was significantly lower than that of experimental group 4, and Figure 2 It can be seen that some seedlings are turning yellow and withering, which means that the pea vine extract obtained by PE-2 without salt water immersion has a certain inhibitory effect on the growth of dew grass seedlings.

[0055] (2) Each group of dew grass began to bloom about 3 months after transplanting ( Figure 3 The figure shows the flowering of experimental group 1 after transplanting). On November 15, 2024, the dew grass of each group was harvested, the upper stems and leaves were removed, the roots were retained, and the soil at the roots was removed as much as possible. Ten plants were randomly selected from each group, and the number of roots, root length (based on the longest root of a single plant) and root weight (fresh weight) were counted (average value was taken). The final results are shown in Table 3 below:

[0056] Table 3

[0057]

[0058] From the above table 3 and Figure 4-6As shown, in experimental group 4, the seeds were not fumigated, and pea vine extract was not added to the subsequent water fertilizer and supplementary fertilizer, which was equivalent to the blank control group. The root growth of dew grass in experimental groups 1 and 2 had significant improvements in root number, root length and root weight compared with experimental group 4. The early fumigation of seeds and the subsequent use of PE-1 in experimental groups 1 and 2 can significantly improve root growth; although the root growth of experimental group 3 was improved compared with experimental group 4, there were large differences in root number and root weight compared with experimental groups 1 and 2. The high concentration of α-pineneol fumigation seeds (1g / L) in experimental group 3 was not as effective as the 0.2-0.5g / L fumigation in experimental groups 1-2; low concentration (50mg / L) PE-1 was used to assist planting in experimental group 5 , and finally, there was little difference in the root growth of dew grass compared with experimental group 4, that is, low concentration of PE-1 had little effect on the root growth of dew grass; in experimental group 6, high concentration (300 mg / L) PE-1 was used to assist planting, and the final root growth effect was significantly higher than that of experimental group 4, but it was still lower than that of experimental groups 1-2; the seeds of experimental group 7 were not fumigated, and PE-1 was used for subsequent planting. The overall root growth was significantly higher than that of experimental group 4, but slightly lower than that of experimental groups 1-2; although the seeds of experimental group 8 were fumigated, PE-2 (pea vine extract prepared without salt water treatment) was used for subsequent planting, and the overall root growth effect was significantly lower than that of experimental group 4, that is, PE-2 had a certain inhibitory effect on the root growth of dew grass.

[0059] (3) The roots of the dew grass harvested by the method described in the experimental groups 1-8 were washed and placed in a hot air dryer to dry the roots in a hot air environment at 50°C until the moisture content was ≤12%. The β-ecdysone in the roots of the dew grass of each group after drying was then detected. The specific content is shown in Table 4 below:

[0060] Table 4

[0061]

[0062] As can be seen from Table 4 above, the content of β-ecdysone in the dry weight of dew grass roots in experimental group 4 (equivalent to the control group) was 3.24%, while the content of experimental groups 1 and 2 reached more than 5.5%, which was significantly improved compared with experimental group 4; in experimental group 3, high concentration of α-pineneol was used to fumigate seeds (1g / L), and the final content of β-ecdysone was higher than that of experimental group 4, but significantly lower than that of experimental groups 1-2; in experimental groups 5-6, lower and higher concentrations of PE-1 were used for treatment, respectively, but the final content of β-ecdysone was slightly different from that of experimental groups 1-2; in experimental group 7, the seeds were not fumigated, and the final content of β-ecdysone was slightly different from that of experimental group 4; and in experimental group 8, fumigation was carried out, although PE-2 was used for subsequent treatment, the overall content of β-ecdysone was still improved compared with experimental group 4.

[0063] In summary, the present application uses a certain concentration of α-pineneol to fumigate seeds, and the subsequent use of pea vine extract obtained after salt water treatment to assist planting can significantly improve the root growth effect of dew grass, increase the yield while increasing the content of β-ecdysone in the roots, and comprehensively improve the economic benefits of planting.

[0064] The above embodiments are only used to illustrate the technical solutions of the present invention, rather than to limit the same. Although the present invention has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that the technical solutions described in the aforementioned embodiments may still be modified, or some of the technical features may be replaced by equivalents. However, these modifications or replacements do not deviate the essence of the corresponding technical solutions from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims

1. A method for artificial planting and management of dew grass, characterized in that: The artificial planting management method comprises the following steps: S1. Seed pretreatment: After sterilizing mature dew grass seeds, fumigate them in an environment with an α-terpineol content of 0.2-0.5 g / L for 1-2 hours to obtain pretreated seeds for later use; S2. Seed sowing treatment: sterilize the planting soil and add basal fertilizer, then sow the pretreated seeds and irrigate them with clean water; S3. Preparation of pea vine extract: cutting pea vine into segments, mixing with 1%-1.5% saline solution, treating at 50-60° C. for 15-20 min, filtering, adding clean water to the pea vine, grinding and filtering, and drying the filtrate to obtain the pea vine extract; S4. Seedling management: After sowing and germination, water thoroughly once with a fertilizer containing 100-200 mg / L pea vine extract and 10-15 g / L urea; S5. Transplantation management: fertilize the transplanted field and then plow it, then transplant the dew grass seedlings that have grown to 5-6 leaves into the field, and use 100-200 mg / L pea vine extract as rooting water to irrigate thoroughly; S6. Fertilization management: conventional fertilization treatment, and supplementary watering with pea vine extract at a concentration of 100-200 mg / L 2-3 months after transplanting.

2. The method for artificial planting and management of dew grass according to claim 1, characterized in that: The method of disinfecting the dew grass seeds in step S1 is to first air-dry them for 2-3 days, and then soak them in a 3000-5000 times solution of potassium permanganate or a 1000-5000 times solution of thiophanate-methyl for 5-8 hours.

3. The method for artificial planting and management of dew grass according to claim 1, characterized in that: In step S2, the planting soil is selected from at least one of ordinary garden soil, red soil, brown soil or sandy loam, and the planting soil is disinfected by spraying a potassium permanganate solution with a concentration of 0.05%-0.1%, then covered with a film and left to stand for 1-2 days, then removed from the film and naturally aired for 5-7 days.

4. The method for artificial planting and management of dew grass according to claim 1, characterized in that: The base fertilizer in step S2 is manure, and the mixing ratio of planting soil and base fertilizer is 4-6:

1.

5. The method for artificial planting and management of dew grass according to claim 1, characterized in that: In step S3, the mass ratio of adding clean water to the pea vine for grinding and filtering is 1:3, and the grinding and filtering method is grinding at a speed of 12000-18000 r / min for 5-10 minutes.

6. The method for artificial planting and management of dew grass according to claim 1, characterized in that: Control the temperature of seeds during sowing and germination at 12-20℃.

7. The method for artificial planting and management of dew grass according to claim 1, characterized in that: The timing of using water and fertilizer in step S4 is after the emergence rate reaches 50% or more.

8. The method for artificial planting and management of dew grass according to claim 1, characterized in that: In step S5, the amount of field fertilization is 1200-1500 kg / mu, and the fertilizer for field fertilization is a mixture of manure, superphosphate, plant ash and potassium sulfate in a mass ratio of 100-120: 0.3-0.5: 0.4-0.6: 0.1-0.

3.

9. The method for artificial planting and management of dew grass according to claim 1, characterized in that: The conventional fertilization treatment in step S6 is to use manure water + 10-15g / L urea as fertilizer one month and three months after transplanting.

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