Micro-crystallized glycerophosphorylcholine-polysaccharide compound as well as preparation method and application thereof

Abstract

The invention provides a microcrystalline glycerophosphorylcholine-polysaccharide compound and a preparation method and application thereof.The microcrystalline glycerophosphorylcholine-polysaccharide compound comprises glycerophosphorylcholine and polysaccharide, and the polysaccharide is inulin or beta-glucan or a mixture of the inulin and the beta-glucan. A glycerophosphorylcholine solution and a polysaccharide solution are mixed to form networked glycerophosphorylcholine-polysaccharide gel, the networked glycerophosphorylcholine-polysaccharide gel is dehydrated under different temperature gradients through a fluidized bed, and the microcrystalline glycerophosphorylcholine-polysaccharide compound is formed. The problems that the glycerophosphorylcholine is easy to wet, agglomerate and poor in stability in milk powder, solid beverages and tablets are solved.

Description

Technical Field

[0001] The present application belongs to the field of food technology, and specifically relates to a microcrystalline glycerol phosphorylcholine-polysaccharide complex and a preparation method and application thereof. Background Art

[0002] Glycerophosphatidylcholine (GPC), also known as glycerophosphocholine or choline glycerol, is an important phospholipid metabolite and biosynthetic precursor of the neurotransmitter acetylcholine (acetylcholine), and plays multiple physiological functions in the human body. As a biosynthetic precursor of acetylcholine and phosphatidylcholine (PC), GPC can cross the blood-brain barrier and enter the brain, providing the raw material choline for the synthesis of acetylcholine and phosphatidylcholine (PC). GPC can not only improve people's memory and cognition, but also has significant therapeutic effects on brain circulatory decline and Alzheimer's disease, and is called the "anti-aging nutrient" of the brain. GPC has obvious therapeutic effects in the treatment of neurodegenerative diseases such as cerebral ischemic stroke, Alzheimer's disease, and multiple cerebral infarction dementia, with few side effects and good tolerance.

[0003] Chinese patent application number 202410519139.0 discloses a method for preparing glycerophosphocholine concentrated powder from egg yolk. The method uses fresh egg yolk liquid as the starting material, dilutes the egg yolk liquid, adds phospholipase A1 and lysophospholipase, and then hydrolyzes it under emulsified stirring. The egg yolk hydrolyzate is spray-dried, deoiled with supercritical CO2, extracted with hot water, vacuum concentrated and spray-dried to obtain glycerophosphocholine concentrated powder.

[0004] Chinese patent application number 201210069221.5 discloses an L-α-glycerophosphocholine film-coated tablet and a preparation method thereof. The L-α-glycerophosphocholine film-coated tablet consists of an L-α-glycerophosphocholine tablet core and a coating layer. The L-α-glycerophosphocholine tablet core consists of L-α-glycerophosphocholine, a filler, a glidant, and a lubricant. The coating layer is divided into two layers, namely, an isolation coating layer and a moisture-proof coating layer. The L-α-glycerophosphocholine tablet core is prepared by a direct tableting process through prescription screening. The isolation layer coating material and the moisture-proof layer coating material are uniformly sprayed on the surface of the L-α-glycerophosphocholine tablet core by a spray gun.

[0005] Chinese patent application number 202411172052.7 discloses an antioxidant soft capsule for protecting nerve cells and a preparation process thereof. The soft capsule comprises a soft capsule shell and capsule contents. The capsule contents comprise glycerophosphocholine, algal oil DHA, ergothioneine and pyrroloquinoline quinone. The soft capsule shell is prepared by gelatin, purified water and glycerol.

[0006] However, glycerophosphocholine has high water solubility and hygroscopicity. GPC easily absorbs moisture in the air, causing its physical state to change, such as changing from powder to agglomerate or viscous. For the development of tablets, granules and solid beverages, the hygroscopic properties of GPC make it extremely unstable during the shelf life of the finished product. At the same time, the aqueous solution of glycerophosphocholine is stable, and the potency gradually decreases in acidic or alkaline conditions, especially in alkaline solutions. In the development of milk powder, solid beverages and tablets, the above-mentioned instability of glycerophosphocholine is a problem that needs to be solved urgently.

[0007] Inulin is a soluble dietary fiber and a natural fructose polymer consisting of fructose molecules connected by β-glycosidic bonds. As a prebiotic, inulin has a prominent role in regulating intestinal microbiota by stimulating the growth of beneficial bacteria. In addition, inulin also shows excellent health benefits in regulating lipid metabolism, losing weight, lowering blood sugar, inhibiting the expression of inflammatory factors, enhancing mineral absorption, and improving constipation.

[0008] β-Glucan is a natural product, a polysaccharide composed of glucose molecules connected by β-1,3-bonds and β-1,6-bonds. It exists in many plants and fungi, such as mushrooms, yeast, algae, etc. β-Glucan plays an important role in the immune system. It can enhance the activity of immune cells, promote the proliferation and differentiation of immune cells, and improve the ability of immune cells to recognize and eliminate pathogens. In addition, β-Glucan also has multiple effects such as anti-inflammatory, anti-tumor, and antioxidant, which can regulate the function of the immune system and maintain immune balance.

[0009] In the production process of natural inulin and β-glucan, spray drying or freeze drying is used. Water molecules evaporate rapidly in a very short time, and most of the inulin molecules in the aqueous solution do not have enough time to rearrange. Therefore, the solid is mainly amorphous with a small amount of crystal state, such as Figure 3 As shown in the figure, this solid in an amorphous state presents a non-equilibrium dynamic structure and easily absorbs moisture and transforms into a crystalline state. Therefore, ordinary inulin and β-glucan have strong hygroscopicity. Summary of the invention

[0010] In view of the problems existing in the prior art, the present invention provides a microcrystalline glycerol phosphorylcholine-polysaccharide complex and a preparation method and application thereof, which are used to solve the problem that glycerol phosphorylcholine and polysaccharide are easy to absorb moisture.

[0011] The present invention is achieved through the following technical solutions.

[0012] The first object of the present invention is to provide a microcrystalline glycerol phosphorylcholine-polysaccharide complex, characterized in that it comprises glycerol phosphorylcholine and polysaccharide, and the weight ratio of glycerol phosphorylcholine to polysaccharide is 1:1-10, preferably 1:1-5.

[0013] As a further technical solution, the polysaccharide is any one of inulin and β-glucan; the polysaccharide is a mixture of inulin and β-glucan, and the weight ratio of inulin to β-glucan is 1:1-10.

[0014] As a further technical solution, the average degree of polymerization of inulin is 10-60, preferably 15-30, and the average degree of polymerization of β-glucan is 50-200, preferably 100-150.

[0015] As a further technical solution, the glycerol phosphorylcholine-polysaccharide complex is obtained by dehydrating a network gel formed by a polysaccharide solution and a glycerol phosphorylcholine solution under different temperature gradients in a fluidized bed, and the heating process of the fluidized bed is: the induced air temperature is 20-30°C, the time is 10-120 minutes, the induced air temperature is 30-40°C, the time is 10-120 minutes, the induced air temperature is 40-50°C, the time is 10-120 minutes, the induced air temperature is 50-60°C, the time is 10-120 minutes, the induced air temperature is 60-70°C, the time is 10-120 minutes, the induced air temperature is 70-80°C, the time is 10-120 minutes, the induced air temperature is 80-90°C, the time is 10-120 minutes, the induced air temperature is 90-100°C, the time is 10-120 minutes,

[0016] Preferably, the induced air temperature is 30-40°C, the time is 30-60 minutes, the induced air temperature is 40-50°C, the time is 30-60 minutes, the induced air temperature is 50-60°C, the time is 30-60 minutes, the induced air temperature is 60-70°C, the time is 30-60 minutes, the induced air temperature is 70-80°C, the time is 30-60 minutes,

[0017] More preferably, the induced air temperature is 25°C, the time is 10-120 minutes, the induced air temperature is 35°C, the time is 10-120 minutes, the induced air temperature is 45°C, the time is 10-120 minutes, the induced air temperature is 55°C, the time is 10-120 minutes, the induced air temperature is 65°C, the time is 10-120 minutes, the induced air temperature is 75°C, the time is 10-120 minutes, the induced air temperature is 85°C, the time is 10-120 minutes, the induced air temperature is 95°C, the time is 10-120 minutes,

[0018] Most preferably, the induced draft temperature is 35°C, the time is 30-60 minutes, the induced draft temperature is 45°C, the time is 30-60 minutes, the induced draft temperature is 55°C, the time is 30-60 minutes, the induced draft temperature is 65°C, the time is 30-60 minutes, and the induced draft temperature is 75°C, the time is 30-60 minutes.

[0019] The second object of the present invention is to provide a method for preparing microcrystalline glycerol phosphorylcholine-polysaccharide complex, the method comprising:

[0020] S1. preparing a glycerol phosphorylcholine solution;

[0021] S2, preparing a polysaccharide solution;

[0022] S3, adding the glycerol phosphorylcholine solution prepared in step S1 to the polysaccharide solution prepared in step S2 to form a mixed solution, keeping warm and standing, the mixed solution changes from a flowing liquid state to a non-flowing gel state, and obtaining a networked glycerol phosphorylcholine-polysaccharide gel;

[0023] S4, dehydrating the glycerol phosphorylcholine-polysaccharide gel prepared in step S3 under different temperature gradients in a fluidized bed to form a microcrystalline glycerol phosphorylcholine-polysaccharide complex.

[0024] As a further technical solution, in step S2, the preparation process of the polysaccharide solution includes: dissolving the polysaccharide in water at a dissolution temperature of 35-95°C, preferably 45-75°C, and keeping warm for 0.5-10 hours, preferably 1-3 hours, to form a polysaccharide solution with a concentration of 10-35%, preferably 15-30%.

[0025] As a further technical solution, the polysaccharide is any one of inulin and β-glucan; the polysaccharide is a mixture of inulin and β-glucan, and the weight ratio of inulin to β-glucan is 1:1-10.

[0026] As a further technical solution, in step S3, the concentration of the mixed solution is 10-62.5%, preferably 20-40%, the weight ratio of glycerolphosphorylcholine to polysaccharide in the mixed solution is 1:1-10, and the mixed solution is allowed to stand at 5-30°C, preferably 10-20°C for 6-72 hours, preferably 12-24 hours.

[0027] As a further technical solution, in step S4, the temperature rising process of the fluidized bed is as follows: the induced air temperature is 20-30°C, the time is 10-120 minutes, the induced air temperature is 30-40°C, the time is 10-120 minutes, the induced air temperature is 40-50°C, the time is 10-120 minutes, the induced air temperature is 50-60°C, the time is 10-120 minutes, the induced air temperature is 60-70°C, the time is 10-120 minutes, the induced air temperature is 70-80°C, the time is 10-120 minutes, the induced air temperature is 80-90°C, the time is 10-120 minutes, the induced air temperature is 90-100°C, the time is 10-120 minutes,

[0028] Preferably, the induced air temperature is 30-40°C, the time is 30-60 minutes, the induced air temperature is 40-50°C, the time is 30-60 minutes, the induced air temperature is 50-60°C, the time is 30-60 minutes, the induced air temperature is 60-70°C, the time is 30-60 minutes, the induced air temperature is 70-80°C, the time is 30-60 minutes,

[0029] More preferably, the induced air temperature is 25°C, the time is 10-120 minutes, the induced air temperature is 35°C, the time is 10-120 minutes, the induced air temperature is 45°C, the time is 10-120 minutes, the induced air temperature is 55°C, the time is 10-120 minutes, the induced air temperature is 65°C, the time is 10-120 minutes, the induced air temperature is 75°C, the time is 10-120 minutes, the induced air temperature is 85°C, the time is 10-120 minutes, the induced air temperature is 95°C, the time is 10-120 minutes,

[0030] Most preferably, the induced draft temperature is 35°C, the time is 30-60 minutes, the induced draft temperature is 45°C, the time is 30-60 minutes, the induced draft temperature is 55°C, the time is 30-60 minutes, the induced draft temperature is 65°C, the time is 30-60 minutes, and the induced draft temperature is 75°C, the time is 30-60 minutes.

[0031] As a further technical solution, in step S1, the concentration of the glycerol phosphorylcholine solution is 10-90%, and the dissolution temperature is 25°C-65°C.

[0032] The third object of the present invention is to provide a microcrystalline glycerol phosphorylcholine-polysaccharide complex and the use of the microcrystalline glycerol phosphorylcholine-polysaccharide complex prepared by the above preparation method in milk powder, solid beverage and compressed tablet.

[0033] Compared with the prior art, the present invention has the following advantages and beneficial effects:

[0034] 1. The present invention forms a microcrystalline network structure by inducing molecular interactions within polysaccharides, adsorbing a large amount of glycerol phosphorylcholine to form microcrystalline glycerol phosphorylcholine-polysaccharide powder. This solid, which is mainly crystalline and has a small amount of amorphous state, presents a balanced dynamic structure, solving the problem that glycerol phosphorylcholine and polysaccharides are easy to absorb moisture.

[0035] 2. Microcrystalline glycerol phosphorylcholine-polysaccharide complex, due to the network structure formed by the polysaccharide, protects glycerol phosphorylcholine, making it difficult for external small molecules such as water or solvents to contact glycerol phosphorylcholine, thereby solving the acid-base instability defect of glycerol phosphorylcholine.

[0036] 3. Microcrystalline glycerol phosphorylcholine-polysaccharide complex, the selected inulin and β-glucan are both high-quality dietary fibers. Inulin is also a high-quality prebiotic. It is currently known to have physiological benefits for intestinal health, sugar and lipid metabolism, obesity, and the immune system. BRIEF DESCRIPTION OF THE DRAWINGS

[0037] Figure 1 This is an electron microscope image of the microcrystalline glycerol phosphorylcholine-polysaccharide complex prepared in Example 5 of the present invention.

[0038] Figure 2 This is the X-ray diffraction pattern for comparing Comparative Example 2 with Example 5.

[0039] Figure 3 It is a schematic diagram of the amorphous state of natural inulin or β-glucan in the prior art.

[0040] Figure 4 Schematic diagram of the process of glycerolphosphorylcholine inducing polysaccharide to form a microcrystalline network. DETAILED DESCRIPTION

[0041] In order to better understand the present invention, a microcrystalline glycerol phosphorylcholine-polysaccharide complex of the present invention and its preparation method and application are described in detail below in combination with examples and comparative examples.

[0042] The object of the present invention is to solve the problem that glyceryl phosphoryl choline is easy to absorb moisture and has poor acid-base stability by preparing a microcrystalline glyceryl phosphoryl choline-polysaccharide complex, and utilize the zwitterionic characteristics and extremely strong water solubility of GPC to form intermolecular hydrogen bonds and gels, thereby enhancing the three-dimensional network structure of the gel. At the same time, in the drying process, by gradient heating, polysaccharides are allowed to have enough time to rearrange, induce molecular interactions to form a microcrystalline network structure, adsorb a large amount of glyceryl phosphoryl choline, and form microcrystalline glyceryl phosphoryl choline-polysaccharide complexes. The selected inulin and β-glucan are both high-quality dietary fibers, with the benefits of regulating the stomach, losing weight, preventing and treating diabetes, preventing and treating cardiovascular diseases, and smooth bowel movements. Inulin is also a high-quality prebiotic at the same time, and it is currently known that there are physiological benefits for intestinal health, sugar and lipid metabolism, obesity, and the immune system.

[0043] It should be noted that the experimental methods used in the examples are conventional methods unless otherwise specified. The materials, reagents, methods and instruments used are conventional materials, reagents, methods and instruments in the art unless otherwise specified, and can be obtained by those skilled in the art through commercial channels.

[0044] equipment:

[0045] ZLG3×0.30 vibrating fluidized bed dryer Jiangsu Pioneer Intelligent Technology Co., Ltd.

[0046] YC-015 Small Spray DryerShanghai Yacheng Instrument Equipment Co., Ltd.

[0047] Example 1

[0048] S1. Prepare a glycerylphosphorylcholine solution: add 10 kg of glycerylphosphorylcholine to 90 kg of distilled water, heat and stir, and form a solution with a concentration of 10% and a dissolution temperature of 25°C.

[0049] S2. Prepare a polysaccharide solution: dissolve 10 kg of inulin with a degree of polymerization of 10 in 90 kg of distilled water, heat and stir, the dissolution temperature is 35° C., keep warm for 0.5 hours, and the solution concentration is 10%.

[0050] S3, gelation: adding the glycerol phosphorylcholine solution prepared in step S1 to the polysaccharide solution prepared in step S2 to form a mixed solution with a total concentration of 10%, wherein the weight ratio of glycerol phosphorylcholine to polysaccharide is 1:1, standing and keeping warm at 5°C for 6 hours, the mixed solution changes from a flowable liquid state to a non-flowing gel state, thereby obtaining a networked glycerol phosphorylcholine-polysaccharide gel.

[0051] S4, microcrystallization: the glycerol phosphorylcholine-polysaccharide gel obtained in step S4 is gradually heated and dehydrated in a fluidized bed to form a microcrystallized glycerol phosphorylcholine-polysaccharide complex, and the control parameters of the fluidized bed are: the induced air temperature is 25°C (10 minutes), 35°C (10 minutes), 45°C (10 minutes), 55°C (10 minutes), 65°C (10 minutes), 75°C (10 minutes), 85°C (10 minutes), 95°C (10 minutes). Microcrystallized glycerol phosphorylcholine-polysaccharide powder is obtained, and the average particle size of the powder is 58 microns.

[0052] The microcrystalline glycerol phosphorylcholine-polysaccharide complex prepared by the above steps has a moisture content of 6.0%. After the hygroscopicity test, the micropowder is in a slightly agglomerated state, and the weight gain percentage is 15.8%.

[0053] Through step S2, the polysaccharide is dissolved, so that the curled polysaccharide molecules are opened in water to become long-chain molecules;

[0054] In step S3, the glycerol phosphorylcholine solution is mixed with the polysaccharide solution to promote the interaction of the internal molecules of the polysaccharide to form a networked gel structure, such as Figure 4As shown in the figure, due to the zwitterionic properties of glycerolphosphorylcholine, GPC is sandwiched between the long-chain molecules of polysaccharides, reducing the mutual electrostatic repulsion between the polysaccharide molecular chains, and can promote the transformation of the polysaccharide molecules in the solution from random coils to helical structures. At the same time, glycerolphosphorylcholine has extremely strong water solubility and is miscible with water. Since glycerolphosphorylcholine competes for water molecules, the molecular solvation degree of polysaccharides is greatly reduced, which is conducive to the formation of intermolecular hydrogen bonds and gels, thereby enhancing the three-dimensional network structure of polysaccharides.

[0055] By increasing the temperature gradually in step S4, the polysaccharide is allowed to have enough time to rearrange itself, inducing molecular interactions to form a microcrystalline network structure, and adsorbing a large amount of glycerolphosphorylcholine. The formed network structure protects the glycerolphosphorylcholine and blocks external water molecules or solvents.

[0056] Embodiment 2-8

[0057] The process of the method is the same as that of Example 1. The polysaccharide types, ratio parameters, and control parameters of the preparation process are shown in Table 1.

[0058] The particle size, moisture content, state of the complex after the hygroscopicity test and weight gain percentage of the microcrystalline glycerol phosphorylcholine-polysaccharide complex prepared by the method of Examples 2-8 are shown in Table 2.

[0059] Comparative Example 1

[0060] A 50% glycerol phosphoryl choline solution was prepared and spray dried to obtain solid glycerol phosphoryl choline. The spray drying conditions were: injection port temperature 190°C, outlet port temperature 80°C, injection speed 1L / hour, and the obtained glycerol phosphoryl choline powder. The state of the composite and the weight gain percentage after the moisture content and hygroscopicity test were shown in Table 2.

[0061] Comparative Example 2

[0062] A 20% inulin solution was prepared, the DP value of inulin was 23, and spray drying was performed. The spray drying conditions were: the injection port temperature was 190°C, the outlet temperature was 80°C, and the injection speed was 1 L / hour to obtain inulin powder. The state of the composite after the moisture content and hygroscopicity test and the weight gain percentage are shown in Table 2. At the same time, X-ray diffraction (XRD) analysis was performed on Example 5 and Comparative Example 2.

[0063] Table 1

[0064]

[0065]

[0066] Table 2

[0067] Group Particle size (micrometer) Moisture content of the compound Hygroscopicity (weight gain percentage) Example 1 58 6.0％ 15.8%, slight clumping Example 2 310 2.2％ 15.0%, slight clumping Example 3 151 3.8％ 14.1%, slight clumping Example 4 223 3.1％ 10.2%, powder, good fluidity Example 5 155 2.6％ 8.2%, powder, excellent fluidity Example 6 180 3.2％ 7.6%, powder, excellent fluidity Example 7 155 3.6％ 9.2%, powder, good fluidity Example 8 63 6.2％ 10.3%, good liquidity Comparative Example 1 152 3.1％ 38.3%, deliquesced and formed liquid Comparative Example 2 158 3.5％ 18.5%, agglomeration

[0068] It can be seen from the comparison between the embodiment and the comparative example that the microcrystallized glycerol phosphoryl choline has not deliquesced, while the spray-dried glycerol phosphoryl choline has strong hygroscopicity and has deliquesced into a liquid state.

[0069] from Figure 2 It can be seen that Example 5 has obvious diffraction peaks compared to Comparative Example 2, indicating that compared with the amorphous inulin in Comparative Example 2, under the condition of similar water content, glycerol phosphorylcholine causes the polysaccharide to undergo obvious crystal transformation in Example 5.

[0070] The determination of particle size, moisture content and hygroscopicity test all adopt conventional techniques well known to those skilled in the art.

[0071] Hygroscopicity test: Guiding principles for hygroscopicity test of drugs 9103 in the 2020 edition of the Chinese Pharmacopoeia.

[0072] The specific test method is as follows.

[0073] 1. Take a dry stoppered glass weighing bottle (outer diameter 50mm, height 15mm), place it in a suitable 25℃±1℃ constant temperature dryer (with ammonium chloride or ammonium sulfate saturated solution placed at the bottom) or artificial climate box (set temperature 25℃±1℃, relative humidity 80%±2%) one day before the test, and accurately weigh the weight (m 1 ).

[0074] 2. Take an appropriate amount of the test sample and spread it flat in the above weighing bottle. The thickness of the test sample is generally about 1mm. Accurately weigh the weight (m 2 ).

[0075] 3. Open the weighing bottle and place it with the bottle cap under the above constant temperature and humidity conditions for 24 hours.

[0076] 4. Cover the weighing bottle and weigh it accurately (m 3 ).

[0077] Weight gain percentage = (m 3 1m 2 ) / (m 2 1m 1 )×100%.

[0078] The above-mentioned embodiments only express several implementation methods of the present invention, and the description thereof is relatively specific and detailed, but it cannot be understood as limiting the scope of the patent application. It should be pointed out that, for a person of ordinary skill in the art, several variations and improvements can be made without departing from the concept of the present invention, and these all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention shall be subject to the attached claims.

Claims

1. A microcrystalline glycerol phosphorylcholine-polysaccharide complex, characterized in that: The invention comprises glycerol phosphoryl choline and polysaccharide, wherein the weight ratio of glycerol phosphoryl choline to polysaccharide is 1:1-10, preferably 1:1-5.

2. The microcrystalline glycerol phosphorylcholine-polysaccharide complex according to claim 1, characterized in that: The polysaccharide is any one of inulin and beta-glucan; the polysaccharide is a mixture of inulin and beta-glucan, and the weight ratio of inulin to beta-glucan is 1:1-10.

3. The microcrystalline glycerol phosphorylcholine-polysaccharide complex according to claim 2, characterized in that: The average degree of polymerization of inulin is 10-60, preferably 15-30, and the average degree of polymerization of β-glucan is 50-200, preferably 100-150.

4. The microcrystalline glycerol phosphorylcholine-polysaccharide complex according to claim 1 or 3, characterized in that: The glycerol phosphorylcholine-polysaccharide complex is obtained by dehydrating a network gel formed by a polysaccharide solution and a glycerol phosphorylcholine solution under different temperature gradients in a fluidized bed. The heating process of the fluidized bed is as follows: the induced air temperature is 20-30°C, the time is 10-120 minutes, the induced air temperature is 30-40°C, the time is 10-120 minutes, the induced air temperature is 40-50°C, the time is 10-120 minutes, the induced air temperature is 50-60°C, the time is 10-120 minutes, the induced air temperature is 60-70°C, the time is 10-120 minutes, the induced air temperature is 70-80°C, the time is 10-120 minutes, the induced air temperature is 80-90°C, the time is 10-120 minutes, the induced air temperature is 90-100°C, the time is 10-120 minutes, Preferably, the induced air temperature is 30-40°C, the time is 30-60 minutes, the induced air temperature is 40-50°C, the time is 30-60 minutes, the induced air temperature is 50-60°C, the time is 30-60 minutes, the induced air temperature is 60-70°C, the time is 30-60 minutes, the induced air temperature is 70-80°C, the time is 30-60 minutes, More preferably, the induced air temperature is 25°C, the time is 10-120 minutes, the induced air temperature is 35°C, the time is 10-120 minutes, the induced air temperature is 45°C, the time is 10-120 minutes, the induced air temperature is 55°C, the time is 10-120 minutes, the induced air temperature is 65°C, the time is 10-120 minutes, the induced air temperature is 75°C, the time is 10-120 minutes, the induced air temperature is 85°C, the time is 10-120 minutes, the induced air temperature is 95°C, the time is 10-120 minutes, Most preferably, the induced draft temperature is 35°C, the time is 30-60 minutes, the induced draft temperature is 45°C, the time is 30-60 minutes, the induced draft temperature is 55°C, the time is 30-60 minutes, the induced draft temperature is 65°C, the time is 30-60 minutes, and the induced draft temperature is 75°C, the time is 30-60 minutes.

5. A method for preparing the microcrystalline glycerol phosphorylcholine-polysaccharide complex according to any one of claims 1 to 4, characterized in that: The method includes: S1. preparing a glycerol phosphorylcholine solution; S2, preparing a polysaccharide solution; S3, adding the glycerol phosphorylcholine solution prepared in step S1 to the polysaccharide solution prepared in step S2 to form a mixed solution, keeping warm and standing, the mixed solution changes from a flowing liquid state to a non-flowing gel state, and obtaining a networked glycerol phosphorylcholine-polysaccharide gel; S4, dehydrating the glycerol phosphorylcholine-polysaccharide gel prepared in step S3 under different temperature gradients in a fluidized bed to form a microcrystalline glycerol phosphorylcholine-polysaccharide complex.

6. The method according to claim 5, characterized in that In step S2, the preparation process of the polysaccharide solution includes: dissolving the polysaccharide in water at a dissolution temperature of 35-95°C, preferably 45-75°C, and keeping warm for 0.5-10 hours, preferably 1-3 hours, to form a polysaccharide solution with a concentration of 10-35%, preferably 15-30%.

7. The method according to claim 5 or 6, characterized in that: The polysaccharide is any one of inulin and beta-glucan; the polysaccharide is a mixture of inulin and beta-glucan, and the weight ratio of inulin to beta-glucan is 1:1-10.

8. The method according to claim 5, characterized in that In step S3, the concentration of the mixed solution is 10-62.5%, preferably 20-40%, the weight ratio of glycerophosphorylcholine to polysaccharide in the mixed solution is 1:1-10, and the mixed solution is allowed to stand at 5-30°C, preferably 10-20°C for 6-72 hours, preferably 12-24 hours.

9. The method according to claim 5, characterized in that In step S4, the temperature rising process of the fluidized bed is as follows: the induced air temperature is 20-30°C, the time is 10-120 minutes, the induced air temperature is 30-40°C, the time is 10-120 minutes, the induced air temperature is 40-50°C, the time is 10-120 minutes, the induced air temperature is 50-60°C, the time is 10-120 minutes, the induced air temperature is 60-70°C, the time is 10-120 minutes, the induced air temperature is 70-80°C, the time is 10-120 minutes, the induced air temperature is 80-90°C, the time is 10-120 minutes, the induced air temperature is 90-100°C, the time is 10-120 minutes, Preferably, the induced air temperature is 30-40°C, the time is 30-60 minutes, the induced air temperature is 40-50°C, the time is 30-60 minutes, the induced air temperature is 50-60°C, the time is 30-60 minutes, the induced air temperature is 60-70°C, the time is 30-60 minutes, the induced air temperature is 70-80°C, the time is 30-60 minutes, More preferably, the induced air temperature is 25°C, the time is 10-120 minutes, the induced air temperature is 35°C, the time is 10-120 minutes, the induced air temperature is 45°C, the time is 10-120 minutes, the induced air temperature is 55°C, the time is 10-120 minutes, the induced air temperature is 65°C, the time is 10-120 minutes, the induced air temperature is 75°C, the time is 10-120 minutes, the induced air temperature is 85°C, the time is 10-120 minutes, the induced air temperature is 95°C, the time is 10-120 minutes, Most preferably, the induced draft temperature is 35°C, the time is 30-60 minutes, the induced draft temperature is 45°C, the time is 30-60 minutes, the induced draft temperature is 55°C, the time is 30-60 minutes, the induced draft temperature is 65°C, the time is 30-60 minutes, and the induced draft temperature is 75°C, the time is 30-60 minutes.

10. The method according to claim 5, characterized in that In step S1, the concentration of the glycerol phosphorylcholine solution is 10-90%, and the dissolution temperature is 25°C-65°C.

11. Use of the microcrystalline glycerol phosphorylcholine-polysaccharide complex according to any one of claims 1 to 4 and the microcrystalline glycerol phosphorylcholine-polysaccharide complex produced by the preparation method according to any one of claims 5 to 10 in milk powder, solid beverage and compressed tablet.

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