Biological enzymolysis device and method for preparing protein peptide from colla corii asini byproduct

Abstract

The invention relates to the technical field of biological enzymolysis devices, and particularly discloses a biological enzymolysis device and method for preparing protein peptide from colla corii asini byproducts, the biological enzymolysis device comprises a reaction kettle, biological enzymolysis is carried out in the reaction kettle, the reaction kettle is internally provided with a stirring assembly, the stirring assembly comprises blades, and the blades rotate around the center of the reaction kettle through a rotating ring; the rotating ring can move up and down in the axial direction of the reaction kettle and moves up and down through a hydraulic structure; the rotation limiting structure is mounted between the blades and the rotating ring and realizes autorotation of the blades through a driving source; height adjustment and rotation / revolution mode switching of the blades are achieved through hydraulic drive, when materials sink, the blades descend to the bottom and are meshed with the gear ring, rotation actions are superposed on a revolution track, a three-dimensional shear force field is formed, the turbulence effect is further generated through the flow channel design of the guide pipe and the flow guide roller, directional impact is applied to bottom sediment, and the material is discharged. The problem that a traditional stirrer is insufficient in acting force on lower-layer materials is solved.

Description

Technical Field

[0001] The present invention relates to the technical field of biological enzymolysis devices, and particularly to a biological enzymolysis device and method for preparing protein peptides by using by-products of donkey-hide gelatin. Background Art

[0002] A biological enzymolysis device for preparing protein peptides by using by-products of donkey-hide gelatin is a device specifically used for converting by-products of donkey-hide gelatin (such as collagen) into small molecule protein peptides through an enzymolysis reaction, usually carried out in a reaction kettle; For example, the prior art publication number CN113244870 discloses a reaction kettle for cooling viscous materials. In this patent, the driving motor works to drive the driving gear to rotate, the driving gear drives the driven gear to rotate, and the driven gear drives the stirring mechanism to work. When starting, through the cooperation of the driving component and the liquid pressure in the driving cavity, and through the transmission of the transmission component, the stirring mechanism is controlled to be in the starting state. On the one hand, it can reduce the resistance suffered when the equipment starts and improve the service life of the equipment. On the other hand, it is in a stirring state, facilitating the stirring of the internal materials. When the materials complete the stirring reaction and need to be cooled, first, the cooling liquid is circulated through the water inlet pipe and the water outlet pipe in the cooling cavity, and then, through the cooperation of the driving component and the liquid pressure in the driving cavity, and through the transmission of the transmission component, the stirring mechanism is controlled to be in the cooling state. During the rotation of the stirring mechanism, the viscous materials are continuously coated on the inner wall of the reaction kettle, improving the heat exchange efficiency with the cooling liquid and the cooling efficiency. Moreover, the cooling liquid can be synchronously introduced into the driving cavity to cool from multiple directions around and above and below the reaction kettle. Compared with the prior art, it improves the cooling efficiency of the materials and solves the technical problems of poor cooling effect, heat energy waste, and large load in the prior art reaction kettle; The fixed stirrer in the above prior art cannot adjust the working height, resulting in weak shear force on the lower-layer materials. The prior art relies on increasing the overall rotation speed to increase the mixing intensity, but this will exacerbate the turbulent dissipation of the upper-layer fluid. Summary of the Invention

[0003] The purpose of this part is to outline some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this part, as well as in the abstract and title of the specification of this application, to avoid obscuring the purpose of this part, the abstract, and the title of the invention. However, such simplifications or omissions shall not be used to limit the scope of the present invention.

[0004] The present invention provides a biological enzymolysis device and method for preparing protein peptides by using by-products of donkey-hide gelatin, which can solve the problem of the prior art "relying on increasing the overall rotation speed to increase the mixing intensity, but this will exacerbate the turbulent dissipation of the upper-layer fluid". The specific solutions are as follows: In one aspect, the present invention provides a bio-enzymatic hydrolysis device for preparing protein peptides using donkey-hide gelatin byproducts, comprising a reactor, wherein the bio-enzymatic hydrolysis is performed in the reactor, and the reactor is provided with a stirring component, wherein the stirring component comprises: The blades rotate around the center of the reactor through a rotating ring, and the rotating ring can move up and down along the axis of the reactor. The rotating ring realizes the up and down movement through a hydraulic structure; The rotation limiting structure is installed between the blade and the rotating ring, and realizes the self-rotation of the blade through the driving source; The hydraulic structure controls the up and down movement of the rotating ring. When the rotating ring is below the reactor, the driving source on the blade drives the blade to rotate, thereby superimposing the rotation action on the revolution trajectory of the blade to form a three-dimensional shearing position to stir the mixed liquid at the bottom of the reactor. Height adjustment and rotation / revolution mode switching are achieved through hydraulic drive. When the material sinks, the blades drop to the bottom and mesh with the gear ring, superimposing the rotation action on the revolution trajectory to form a three-dimensional shear force field. The flow channel design of the guide pipe and guide roller further produces a turbulent effect, exerting a directional impact on the bottom sediment, solving the problem of insufficient force of traditional agitators on the lower layer of materials.

[0005] Preferably, one end of the blade close to the rotating ring is connected to a first gear, and a gear ring is installed on the hydraulic structure. When the rotating ring and the first gear move driven by the hydraulic structure, the bottom of the gear ring abuts against the fixed structure in the reactor to stop the gear ring from descending. At this time, the rotating ring and the first gear continue to descend, thereby allowing the first gear and the gear ring to mesh and realize the orbital revolution of the blade.

[0006] Preferably, a conical sleeve is installed on the top of the rotating ring, and the conical sleeve is installed on the top of the reaction kettle to achieve the function of receiving the enzyme species.

[0007] Preferably, the outer diameter of the tapered sleeve is set to gradually expand, and the tapered sleeve is fixedly mounted on the rotating ring and rotates synchronously with the rotating ring; Through the design of the conical sleeve and its rotating structure, the enzyme can be directed to the side wall of the reactor rather than the central area when it is added, and the centrifugal force generated by the revolution of the blades forms a dynamic diffusion path. When the conical sleeve rotates synchronously with the gear sleeve, the enzyme is dispersed in a vortex along its gradually expanding outer diameter, effectively avoiding local accumulation. This structure breaks through the limitation of traditional vertical feeding that is prone to agglomeration, maximizes the contact area between the enzyme and the raw material, and significantly shortens the mixing time.

[0008] Preferably, two guide tubes are installed on the blade, one end of the two guide tubes is close to the meeting surface of the blade and the liquid, and the other end of the two guide tubes diffuses with each other.

[0009] Preferably, a guide roller is installed at one end of the blade, and an arc-shaped piece is installed at the lower end of the guide roller. The blade is fixed to the blade and configured such that water can only pass above the guide roller, thereby driving the guide roller to rotate to one side. When the guide roller rotates, it can disturb the liquid near the guide roller.

[0010] Preferably, there is a reset structure between the first gear and the rotating ring. The reset structure includes: A spiral groove is opened at one end of the blade; A telescopic member is fixed to the rotating ring; It is configured that when the blade needs to rotate self, the extending end of the telescopic member leaves the spiral groove, and when the blade needs to be fixed to the rotating ring, the extending end of the telescopic member penetrates into the innermost part of the spiral groove to keep the blade at a fixed angle.

[0011] Preferably, a toothed sleeve is provided at the top of the rotating ring, and a second gear is provided inside the toothed sleeve. The second gear rotates under the drive of a drive device. The second gear and the toothed sleeve are driven by the drive device to rotate the second gear, and the second gear drives the toothed sleeve to rotate, so as to drive the whole rotating ring and the blade to rotate.

[0012] Preferably, a temperature sensor, a pH value sensor and a degree of hydrolysis sensor are installed inside the reaction kettle.

[0013] On the other hand, the present invention provides a biological enzymatic hydrolysis method for preparing protein peptides from donkey-hide gelatin by-products, including the following steps: S1. The hydraulic structure controls the rotating ring with blades to move up and down along the axial direction of the reaction kettle; when the rotating ring moves to the lower area of the reaction kettle, the drive source installed between the blade and the rotating ring is started; S2. The drive source drives the blade to generate a self-rotation movement while revolving around the center of the reaction kettle, so that the revolution trajectory of the blade is superimposed with the self-rotation action; S3. Using the three-dimensional shear force field generated by the superimposed movement, the mixed liquid in the lower area of the reaction kettle is stirred by enzymatic hydrolysis reaction. The rotation speed of the self-rotation movement and the rotation speed of the revolution movement form a speed gradient difference. By adjusting the lifting frequency of the hydraulic structure and the rotation speed parameters of the drive source, the intensity distribution of the three-dimensional shear force field is controlled.

[0014] Compared with the prior art, the present invention can at least achieve one of the following beneficial effects: 1. The blade realizes height adjustment and self-rotation / revolution mode switching through hydraulic drive. When the material sinks, the blade descends to the bottom and meshes with the toothed ring, and a self-rotation action is superimposed on the revolution trajectory to form a three-dimensional shear force field. The flow channel design of the guide pipe and the guide roller further generates a turbulent effect, exerting a directional impact on the bottom sediment, and solving the problem of insufficient force on the lower-layer material by the traditional stirrer.

[0015] 2. The split - type drive design is adopted, and the meshing drive between the gear sleeve and the rotating ring is independent of the hydraulic lifting system. The motor drives the revolution through the eccentric gear set, while the hydraulic system realizes the height adjustment. The two do not interfere with each other and are convenient for maintenance. It can be flexibly adapted to various enzymatic hydrolysis processes such as alkaline protease and trypsin, significantly reducing the equipment transformation cost.

[0016] 3. A composite flow channel system is formed by the guide pipe on the blade and the guide roller. Through the converging effect of the upstream surface and the diffusion effect of the downstream surface, an asymmetric fluid field is constructed. The guide pipe directs the high - speed jet into the material accumulation area, and the rotation of the guide roller disturbs and breaks the boundary - layer stagnant flow. The double - effect superposition significantly improves the liquid - solid mass transfer rate.

[0017] 4. Through the design of the conical sleeve and its rotating structure, when the enzyme species are put in, they can be guided to the side wall of the reaction kettle instead of the central area. Combining with the centrifugal force generated by the revolution of the blade, a dynamic diffusion path is formed. When the conical sleeve rotates synchronously with the gear sleeve, the enzyme species are dispersed in a vortex shape along its gradually expanding outer diameter, effectively avoiding local accumulation. This structure breaks through the limitation of the traditional vertical feeding which is easy to form lumps, maximizes the contact area between the enzyme and the raw materials, and significantly shortens the mixing time.

[0018] Other features and advantages of the present invention will be described in the following specification, and some of them will become obvious from the specification, or be understood by implementing the present invention. The objectives and other advantages of the present invention can be achieved and obtained through the structures specifically pointed out in the written specification and the drawings. Brief Description of the Drawings

[0019] To more clearly illustrate the technical solutions of the embodiments of the present invention, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following - described drawings are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings. Among them: Figure 1 is the overall three - dimensional view of the present invention; Figure 2 is the overall partial cross - sectional view of the present invention; Figure 3 is the front cross - sectional view of the present invention; Figure 4 is the cross - sectional view of the stirring assembly of the present invention; Figure 5 is the overall three - dimensional cross - sectional view of the present invention; Figure 6 is of the present invention Figure 5 The enlarged view at A in; Figure 7 is the structural schematic diagram of the spiral groove of the present invention; Figure 8This is the first - perspective three - dimensional view of the stirring component of the present invention; Figure 9 This is the second - perspective three - dimensional view of the stirring component of the present invention; Figure 10 This is the third - perspective three - dimensional view of the stirring component of the present invention; Figure 11 This is the three - dimensional view of the blade of the present invention; Figure 12 This is the partial cross - sectional view of the blade of the present invention; Figure 13 This is the lower - part schematic view of the blade of the present invention.

[0020] Among them, the reference numerals are as follows: 100, reaction kettle; 101, enzymatic hydrolysis kettle body; 102, first feed pipe; 103, discharge pipe; 104, second feed pipe; 200, stirring component; 201, fixed column; 202, conical sleeve; 203, blade; 204, rotating ring; 205, limiting sleeve; 206, limiting ring; 207, cavity; 208, angle sensor; 209, telescopic member; 210, spiral groove; 301, hydraulic sleeve; 302, hydraulic pipe; 303, telescopic sleeve; 304, support plate; 305, toothed ring; 306, limiting rod; 307, first gear; 401, toothed sleeve; 402, driving rod; 403, second gear; 404, motor; 2031, guide pipe; 2032, conical block; 2034, guide roller; 2035, arc - shaped piece. Detailed implementation manners

[0021] The following combines the drawings to specifically describe the preferred embodiments of the present invention. Among them, the drawings form a part of the present invention and are used together with the embodiments of the present invention to explain the principle of the present invention.

[0022] Embodiment 1: As Figure 1As described above, this embodiment provides a biological enzymolysis device for preparing protein peptides from donkey-hide gelatin by-products, including a reaction kettle 100. The reaction kettle 100 includes an enzymolysis kettle body 101, which is used for mixing and reacting raw materials. A first feed pipe 102 is arranged on the top side of the enzymolysis kettle body 101, and the first feed pipe 102 is used for feeding the main raw materials. The raw materials refer to donkey-hide gelatin by-products (the residue of donkey skin after washing, crushing, and degreasing). A discharge pipe 103 is arranged on the bottom side of the enzymolysis kettle body 101, and the discharge pipe 103 is used for discharging the raw materials after the reaction is completed. A second feed pipe 104 is arranged on the top of the enzymolysis kettle body 101, and the second feed pipe 104 is used for feeding enzyme species. The enzyme species can be: alkaline protease, trypsin, composite enzyme, etc. Among them, alkaline protease (Alcalase) is applicable to pH 8-10 and temperature 50-60 °C; trypsin (Trypsin) is applicable to pH 7-9 and temperature 37-45 °C; composite enzyme (such as the combination of alkaline and neutral proteases) can improve the hydrolysis efficiency. Since the temperature and pH value of the reaction need to be controlled when the materials and enzyme species react, among them, the reaction temperature is between 40-60 °C, the pH value is adjusted according to the enzyme species, such as pH 8-10 for alkaline protease, the stirring speed (50-200 rpm) and time (2-8 hours), and the degree of hydrolysis (DH value) is monitored in real time. Therefore, a temperature sensor, a pH value sensor, and a degree of hydrolysis sensor are installed inside the enzymolysis kettle body 101. Electronic valves are installed in the middle of the first feed pipe 102, the discharge pipe 103, and the second feed pipe 104, and the electronic valves can be controlled by a controller. As Figure 2 , Figure 3 As shown in the figure, in this embodiment, a stirring assembly 200 is arranged inside the enzymolysis kettle body 101. The stirring assembly 200 is used for fully mixing the donkey-hide gelatin raw materials and enzyme species, and accelerating the chemical reaction between the two. The specific structure of the stirring assembly 200 will be described below: As Figure 4 , Figure 5 , Figure 6 As shown in the figure, in this embodiment, the stirring assembly 200 specifically includes: A fixed column 201, installed inside the enzymolysis kettle body 101 and located at the middle position of the enzymolysis kettle body 101. A conical sleeve 202 is arranged on the fixed column 201. The conical sleeve 202 is installed on the upper outer wall of the fixed column 201 in a fixed or rotatable manner. The function of the conical sleeve 202 is that when the enzyme species are put in from the second feed pipe 104 above the enzymolysis kettle body 101, under the action of the conical sleeve 202, the enzyme species can fall on the side wall position of the enzymolysis kettle body 101, avoiding falling on the middle position of the enzymolysis kettle body 101. The advantage of the enzyme species falling on the side wall position of the enzymolysis kettle body 101 is that under the operation of the stirring assembly 200, the enzyme species can be repeatedly mixed with the raw materials, thereby improving the chemical reaction efficiency. The blade 203, which can be single or multiple, is installed on the outer wall of the fixed column 201. A rotating ring 204 is provided at the convergence of one or more blades 203. One end of the blade 203 can be fixed to the rotating ring 204 or can rotate on its own. Among them, the rotating ring 204 is rotatably installed on the outer wall of the fixed column 201. Driven by a drive source, the rotating ring 204 can rotate around the central axis of the fixed column 201. The specific structure of the drive source will be described below. As Figure 6 shown, this embodiment describes the setting method of the rotational connection between the blade 203 and the rotating ring 204. The specific setting is: A limit sleeve 205 is connected to one end of the blade 203 close to the rotating ring 204, and a limit ring 206 is connected to the corresponding position on the rotating ring 204. Among them, the limit sleeve 205 is rotatably connected to the limit ring 206, so as to realize the self-rotation of the blade 203. To facilitate the monitoring of the rotation angle of the blade 203, a cavity 207 is opened at one end of the blade 203 close to the limit sleeve 205, and an angle sensor 208 is arranged inside the cavity 207. Among them, the angle sensor 208 is fixed on the rotating ring 204 or the blade 203, as long as it can satisfy that the angle sensor 208 can detect the relative rotation angle change value between the blade 203 and the rotating ring 204. Among them, the blade 203 itself can rotate on its own driven by a drive source. The specific structure of this drive source will be described below. As Figure 6 shown, since the blade 203 can be driven by a drive source, but in some operating states, the blade 203 also needs to be maintained at a specific angle. Therefore, a spiral groove 210 (the specific structure of the spiral groove 210 is as Figure 7 shown) and a telescopic member 209 are opened at one end of the blade 203 (on the inner wall of the cavity 207). Among them, the telescopic member 209 is fixedly connected to the outer wall of the rotating ring 204, and the protruding end of the telescopic member 209 can extend into the inside of the spiral groove 210, and all positions of the spiral groove 210 communicate with the cavity 207. Therefore, when the blade 203 is at any angle, only need to extend the protruding end of the telescopic member 209, and then push against the spiral groove 210 until the protruding end of the telescopic member 209 is pushed to the rightmost end of the spiral groove 210 (this direction is based on Figure 6 ), this state is the default position of the blade 203.

[0023] As Figure 3 、 Figure 4 shown, the drive source for the self-rotation of the blade 203 includes: The hydraulic sleeve 301 is fixedly connected to the bottom of the inner wall of the enzymatic hydrolysis kettle body 101. A hydraulic pipe 302 is connected to the bottom of the hydraulic sleeve 301. The hydraulic pipe 302 is used to inject hydraulic oil into the hydraulic sleeve 301. An expansion sleeve 303 is hermetically and slidably installed inside the hydraulic sleeve 301. The top end of the expansion sleeve 303 is limited and rotatably connected to the bottom of the rotating ring 204 through a limit ring groove structure. A support plate 304 is also connected to the top end of the hydraulic sleeve 301. A limit structure is connected to the bottom end of the expansion sleeve 303, which can prevent the expansion sleeve 303 from separating from the hydraulic sleeve 301. And a spring is also arranged on the outer wall of the expansion sleeve 303, and the spring presses the expansion sleeve 303 downward; The toothed ring 305 is slidably installed on the outer wall of the top of the expansion sleeve 303. A circle of teeth is arranged on the top of the toothed ring 305. A first gear 307 is connected to one end of the blade 203 close to the rotating ring 204. The lower part of the first gear 307 can mesh with the toothed ring 305 to form a coupling connection (since the toothed ring 305 can move relative to the first gear 307, when the toothed ring 305 and the first gear 307 move away from each other, they are not meshed). A limit rod 306 is connected to the bottom of the toothed ring 305. Among them, the limit rod 306 is slidably connected to the support plate 304; In the above solution, when the hydraulic source in the hydraulic sleeve 301 discharges oil outward, the expansion sleeve 303 moves downward due to gravity and the spring. After the expansion sleeve 303 moves downward, the support plate 304 also moves downward at the same time. Since the toothed ring 305 is placed on the support plate 304, the toothed ring 305 also moves downward at the same time. When the bottom end of the limit rod 306 at the bottom of the toothed ring 305 abuts against the top end of the hydraulic sleeve 301, the support plate 304 and the expansion sleeve 303 continue to descend, while the toothed ring 305 stops moving downward due to the action of the limit rod 306. Therefore, after the expansion sleeve 303 continues to descend a certain distance, the teeth on the top of the toothed ring 305 can mesh with the first gear 307. At this time, during the rotation of the rotating ring 204, since the first gear 307 meshes with the toothed ring 305 (the toothed ring 305 is fixed and does not rotate), the first gear 307 and the blade 203 will start to rotate; Through the above solution, when it is necessary to further fully mix the raw materials in the lower part of the enzymatic hydrolysis kettle body 101, the blade 203 and the rotating ring 204 are integrally lowered to the lower part of the enzymatic hydrolysis kettle body 101 through the above hydraulic components. When the blade 203 and the rotating ring 204 descend to the limit position, since the toothed ring 305 is in a rotating state, the first gear 307 on the blade 203 meshes with the toothed ring 305 and also starts to rotate. Therefore, through the rotation and revolution of the blade 203, the raw materials and enzyme species that are easy to sink to the bottom in the lower part of the enzymatic hydrolysis kettle body 101 can be fully mixed, so as to ensure that the raw materials are not easy to sink to the bottom and strengthen the mixing of the materials in the lower part of the enzymatic hydrolysis kettle body 101.

[0024] Such as Figure 4 、 Figure 8, Figure 9 As described above, the driving source for the rotation of the rotating ring 204 includes: A gear sleeve 401, with a circle of teeth penetrating from top to bottom provided on its inner wall; wherein, the bottom of the gear sleeve 401 is fixedly connected to the top of the rotating ring 204; A driving rod 402, rotatably installed at an eccentric position of the fixed column 201, with a second gear 403 fixedly connected to the bottom of the driving rod 402, wherein the second gear 403 meshes with the gear sleeve 401; wherein, a motor 404 is fixedly installed at the top of the driving rod 402, and the output end of the motor 404 is connected to the top end of the driving rod 402. The driving rod 402 is driven to rotate by the motor 404, and then the driving rod 402 drives the second gear 403 to rotate. Since the second gear 403 meshes with the gear sleeve 401, the gear sleeve 401 rotates. Since the gear sleeve 401 is fixedly connected to the rotating ring 204, the rotating ring 204 rotates synchronously, and then the rotating ring 204 drives the blades 203 to revolve.

[0025] As Figure 10 shown, when the tapered sleeve 202 is rotatably installed and fixed on the outer wall of the fixed column 201, the tapered sleeve 202 is fixedly connected to the outer wall of the top of the gear sleeve 401. Since the gear sleeve 401 can rotate relative to the fixed column 201, the tapered sleeve 202 is equivalent to rotating relative to the fixed column 201. In this case, the discharge port of the second feed pipe 104 is above the tapered sleeve 202. Therefore, when the enzyme seeds fall, they can land on the tapered sleeve 202; and in order to further improve the efficiency of mixing the enzyme seeds with the transported material, as Figure 10 shown, the outer diameter of the entire circle of the tapered sleeve 202 is set to be in a gradually expanding trend. The advantage of this is that when the enzyme seeds land on the tapered sleeve 202, since the tapered sleeve 202 rotates synchronously with the gear sleeve 401, the position where the enzyme seeds fall from the edge of the tapered sleeve 202 also changes, and the enzyme seeds will be distributed in a spiral line inside the enzymolysis kettle body 101.

[0026] As Figure 11 , Figure 12 , Figure 13As shown in the figure, in this embodiment, the structure of the blade 203 is further optimized. The specific solution is as follows: Two guiding tubes 2031 are installed on the blade 203. The two guiding tubes 2031 are arranged in an arc shape. And when the blade 203 is in the default state (not rotating), one end of the two guiding tubes 2031 approaches the receiving surface of the blade 203 and the mixed liquid, while the other ends of the two guiding tubes 2031 are arranged to diverge from each other. The effect brought by this solution is that when the blade 203 rotates, a part of the mixed liquid can enter from one end of the guiding tube 2031 (the end where the two guiding tubes 2031 approach each other), and then be discharged from the other end of the guiding tube 2031. Through the gathering effect of the guiding tube 2031, the flow rate of the material discharged from the other end of the guiding tube 2031 is relatively fast, and the impact force of the material with a relatively fast flow rate is also greater. Thus, it can impact other materials inside the enzymatic hydrolysis kettle body 101, causing the materials in the enzymatic hydrolysis kettle body 101 to further disperse, improving the mixing effect and the mixing uniformity. As Figure 12 shown in the figure, in this embodiment, a conical block 2032 is further provided on the top surface of the blade 203. The conical block 2032 can disperse the falling enzyme seeds, so that the enzyme seeds will not aggregate at a certain position to form lumps. And by providing the conical block 2032 on the blade 203, when the blade 203 rotates, under the action of the conical block 2032, the materials on the receiving surface of the blade 203 can also be dispersed. As Figure 13 shown in the figure, in this embodiment, a guide roller 2034 is further provided at one end of the blade 203. An arc-shaped piece 2035 is provided at the bottom end of the blade 203 below the guide roller 2034. The function of the arc-shaped piece 2035 is to prevent the water flow from impacting the area below the guide roller 2034. The large-flow water can only pass above the guide roller 2034, thereby driving the guide roller 2034 to rotate to one side. When the guide roller 2034 rotates, it can disturb the liquid near the guide roller 2034, further improving the mixing efficiency.

[0027] Embodiment 2: The technical solution of this embodiment is different from that of Embodiment 1 in that this embodiment provides a biological enzymatic hydrolysis method for preparing protein peptides from donkey-hide gelatin by-products, including the following steps: S1. The hydraulic structure controls the rotating ring 204 with the blade 203 to move up and down along the axial direction of the reaction kettle 100; when the rotating ring 204 moves to the lower region of the reaction kettle 100, the drive source installed between the blade 203 and the rotating ring 204 is started. S2. The drive source drives the blade 203 to generate a self-rotation movement while revolving around the center of the reaction kettle 100, so that the revolution trajectory of the blade 203 and the self-rotation action are superimposed. S3. Using the three-dimensional shear force field generated by the superimposed motion, perform enzymatic reaction stirring on the mixed liquid in the lower region of the reactor 100. The rotational speed of the self-rotation motion and the rotational speed of the revolution motion form a velocity gradient difference. By adjusting the lifting frequency of the hydraulic structure and the rotational speed parameters of the drive source, control the intensity distribution of the three-dimensional shear force field.

[0028] In summary, the present invention realizes height adjustment and self-rotation / revolution mode switching of the blades through hydraulic drive by setting the blades 203. When the material sinks, the blades 203 descend to the bottom and engage with the toothed ring, superimposing a self-rotation action on the revolution trajectory to form a three-dimensional shear force field. The flow channel design of the guide pipe 2031 and the guide roller 2034 further generates a turbulence effect, exerting a directional impact on the bottom sediment, and solving the problem of insufficient force on the lower-layer materials by the traditional stirrer; adopting a split drive design, the meshing transmission between the gear sleeve and the rotating ring is independent of the hydraulic lifting system. The motor 404 drives the revolution through the eccentric gear set, while the hydraulic system realizes height adjustment. The two do not interfere with each other and are convenient for maintenance, and can be flexibly adapted to various enzymatic hydrolysis processes such as alkaline protease and trypsin, significantly reducing the equipment transformation cost; a composite flow channel system is formed by the guide pipe 2031 and the guide roller 2034 on the blade 203. Through the converging effect of the upstream surface and the diffusion effect of the downstream surface, an asymmetric fluid field is constructed. The guide pipe 2031 directs the high-speed jet into the material accumulation area, and the rotation of the guide roller 2034 disturbs and breaks the boundary layer stagnant flow. The double-effect superposition significantly improves the liquid-solid mass transfer rate; through the design of the tapered sleeve 202 and its rotating structure, the enzyme species can be guided to the side wall of the reactor rather than the central area during enzyme feeding. Combining with the centrifugal force generated by the revolution of the blade 203, a dynamic diffusion path is formed. When the tapered sleeve 202 rotates synchronously with the gear sleeve, the enzyme species are dispersed in a vortex shape along its gradually expanding outer diameter, effectively avoiding local accumulation. This structure breaks through the limitation of easy caking in traditional vertical feeding, maximizes the contact area between the enzyme and the raw material, and significantly shortens the mixing time. In the description of this specification, the description referring to terms such as "one embodiment", "example", "specific example", etc. means that the specific features, structures, materials or characteristics described in connection with the embodiment or example are included in at least one embodiment or example of the present invention. In this specification, the schematic representations of the above terms do not necessarily refer to the same embodiment or example. Moreover, the specific features, structures, materials or characteristics described can be combined in a suitable manner in any one or more embodiments or examples.

[0029] In the description of the specification, claims, and the above-mentioned drawings of the embodiments of the present application, terms such as "first", "second", "third", "fourth", etc. (if any) are used to distinguish similar objects and do not necessarily describe a specific order or sequence. It should be understood that the data used in this way can be interchanged under appropriate circumstances, so that the embodiments of the embodiments of the present application described herein can be implemented in an order other than those illustrated or described herein. In addition, the terms "comprising" and "having" and any variations thereof are intended to cover non-exclusive inclusion. For example, a process, method, system, product, or device that includes a series of steps or units does not necessarily have to be limited to those steps or units clearly listed, but may include other steps or units not clearly listed or inherent to these processes, methods, products, or devices.

[0030] Parallel: The parallelism defined in the present application is not limited to absolute parallelism. The definition of this parallelism can be understood as substantially parallel, allowing for situations where it is not absolutely parallel due to factors such as assembly tolerances, design tolerances, and the influence of structural flatness. Small-angle range errors are allowed. For example, within an assembly error range of less than 10 degrees, it can be understood as a parallel relationship.

[0031] Perpendicular: The perpendicularity defined in the present application is not limited to an absolute perpendicular intersection (an included angle of 90 degrees). It allows for a relationship where it is not an absolute perpendicular intersection due to factors such as assembly tolerances, design tolerances, and the influence of structural flatness. Small-angle range errors are allowed. For example, within an assembly error range of 80 degrees to 100 degrees, it can be understood as a perpendicular relationship.

[0032] In the embodiments of the present application, it is not to be understood that the devices or elements indicated by the embodiments or implications thereof must have a specific orientation, be constructed and operated in a specific orientation, so it cannot be regarded as a limitation to the embodiments of the present application. In the description of the embodiments of the present application, the meaning of "a plurality" is two or more, unless otherwise specifically and precisely defined.

[0033] The preferred embodiments of the present invention disclosed above are only used to help explain the present invention. The preferred embodiments do not elaborate on all the details, nor do they limit the invention to the specific embodiments described. Obviously, many modifications and variations can be made according to the content of this specification. The purpose of selecting and specifically describing these embodiments in this specification is to better explain the principles and practical applications of the present invention, so that those skilled in the art can well understand and utilize the present invention. The present invention is only limited by the claims and their full scope and equivalents.

Claims

1. A bio-enzymatic hydrolysis device for preparing protein peptides using donkey-hide gelatin byproducts, comprising a reactor, wherein the bio-enzymatic hydrolysis is carried out in the reactor, and the reactor is provided with a stirring component, wherein: The stirring assembly includes: The blades rotate around the center of the reactor through a rotating ring, and the rotating ring can move up and down along the axis of the reactor. The rotating ring realizes the up and down movement through a hydraulic structure; The rotation limiting structure is installed between the blade and the rotating ring, and realizes the self-rotation of the blade through the driving source; Among them, the hydraulic structure is used to control the up and down movement of the rotating ring. When the rotating ring is below the reactor, the driving source on the blade drives the blade to rotate, thereby superimposing the rotation action on the orbital trajectory of the blade to form a three-dimensional shearing position to stir the mixed liquid at the bottom of the reactor.

2. A biological enzymatic hydrolysis device for preparing protein peptides using donkey-hide gelatin byproducts as claimed in claim 1, characterized in that: One end of the blade close to the rotating ring is connected to the first gear, and a gear ring is installed on the hydraulic structure. When the rotating ring and the first gear move driven by the hydraulic structure, the bottom of the gear ring abuts against the fixed structure in the reactor to stop the gear ring from descending. At this time, the rotating ring and the first gear continue to descend, so that the first gear and the gear ring engage to realize the orbital revolution of the blade.

3. The bio-enzymatic hydrolysis device for preparing protein peptides using donkey-hide gelatin byproducts as claimed in claim 1, characterized in that: A conical sleeve is installed on the top of the rotating ring, and the conical sleeve is installed on the top of the reaction kettle to achieve the function of receiving the enzyme species.

4. A biological enzymatic hydrolysis device for preparing protein peptides using donkey-hide gelatin byproducts as claimed in claim 2, characterized in that: The outer diameter of the tapered sleeve is set to gradually expand, and the tapered sleeve is fixedly mounted on the rotating ring and rotates synchronously with the rotating ring.

5. The bio-enzymatic hydrolysis device for preparing protein peptides using donkey-hide gelatin byproducts as claimed in claim 1, characterized in that: Two guide tubes are installed on the blades, one end of the two guide tubes is close to the meeting surface of the blades and the liquid, and the other end diffuses with each other.

6. The bio-enzymatic hydrolysis device for preparing protein peptides using donkey-hide gelatin byproducts as claimed in claim 1, characterized in that: A guide roller is installed at one end of the blade, and an arc-shaped sheet is installed at the lower end of the guide roller. The blade is fixed on the blade and configured so that the water flow can only pass over the top of the guide roller, thereby driving the guide roller to rotate to one side. When the guide roller rotates, it can cause disturbance to the liquid near the guide roller.

7. The bio-enzymatic hydrolysis device for preparing protein peptides using donkey-hide gelatin byproducts as claimed in claim 1, characterized in that: A reset structure is provided between the first gear and the rotating ring, and the reset structure comprises: A spiral groove is provided at one end of the blade; A telescopic member, fixed on the rotating ring; The configuration is such that when the blade needs to rotate, the extended end of the telescopic member leaves the spiral groove, and when the blade needs to be fixed to the rotating ring, the extended end of the telescopic member penetrates into the innermost part of the spiral groove to keep the blade at a fixed angle.

8. The bio-enzymatic hydrolysis device for preparing protein peptides from donkey-hide gelatin byproducts as claimed in claim 1, characterized in that: A gear sleeve is provided on the top of the rotating ring, and a second gear is provided on the inner side of the gear sleeve. The second gear rotates under the drive of the driving device. The second gear and the gear sleeve drive the second gear to rotate through the driving device, and the second gear drives the gear sleeve to rotate, so that the rotating ring and the blades rotate as a whole.

9. The bio-enzymatic hydrolysis device for preparing protein peptides using donkey-hide gelatin byproducts as claimed in claim 1, characterized in that: A temperature sensor, a pH sensor and a hydrolysis degree sensor are installed inside the reactor.

10. A biological enzymatic hydrolysis method for preparing protein peptides from donkey-hide gelatin byproducts, using a biological enzymatic hydrolysis device for preparing protein peptides from donkey-hide gelatin byproducts as claimed in any one of claims 1 to 9, characterized in that: The following steps are involved: S1. The hydraulic structure controls the rotating ring with blades to move up and down along the axial direction of the reactor; when the rotating ring moves to the lower area of ​​the reactor, the driving source installed between the blades and the rotating ring is started; S2, the driving source drives the blade to rotate while revolving around the center of the reactor, so that the revolution trajectory of the blade and the rotation motion are superimposed; S3. The three-dimensional shear force field generated by the superimposed motion is used to stir the enzymatic hydrolysis reaction of the mixed liquid in the lower area of ​​the reactor. The speed of the rotational motion and the speed of the revolution motion form a speed gradient difference. The intensity distribution of the three-dimensional shear force field is controlled by adjusting the lifting frequency of the hydraulic structure and the speed parameters of the driving source.

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