Efficient extraction process of papaya protein peptide

Abstract

The invention relates to the technical field of protein peptide extraction, and discloses an efficient papaya protein peptide extraction process which comprises the following steps: cleaning immature green papaya, slicing the green papaya, inactivating endogenous enzyme at high temperature, putting the papaya slices subjected to high-temperature treatment into enzymolysis equipment for stirring and enzymolysis, and filtering to obtain papaya protein peptide; the papaya protein peptide powder is prepared by the following steps: firstly extracting papaya protein peptide from papaya protein peptide, then leading out liquid and residual liquid generated by extruding solid residues through enzymolysis equipment, separating and purifying the liquid through a centrifugal machine to obtain a crude extracting solution, then carrying out ultrafiltration separation on the crude extracting solution to obtain a high-purity peptide solution, and finally, concentrating and spray-drying the high-purity peptide solution to obtain the papaya protein peptide powder. The papaya protein peptide prepared through the method is high in enzymolysis degree, the utilization rate of solid residues and papaya protein peptide is increased in the enzymolysis process, and then the yield and extraction efficiency of papaya protein peptide powder are increased.

Description

Technical Field

[0001] The present invention relates to the technical field of protein peptide extraction, and specifically relates to a high-efficiency extraction process for papain peptides. Background Art

[0002] The composition of protein peptides is centered on amino acids and small peptide chains, and has high bioavailability and multifunctional activities. Its functions cover multiple fields such as nutritional support, immune regulation, chronic disease prevention, beauty and health, etc., and it is an important raw material in the fields of functional foods, health products, and pharmaceuticals;

[0003] In the prior art, a corn protein peptide and its extraction method (publication number CN112592951A) are provided, including the following steps: first, pulverize corn protein powder and sieve it, pre-treat the obtained corn protein fine powder, steam the pre-treated corn protein, add an aqueous solution of sodium sulfite, soak it, filter it, remove the filtrate, add water to the treated corn protein, stir it, add a multifunctional additive to the slurry, stir it, adjust the pH of the mixture, raise the temperature, add alkaline protease, carry out enzymatic hydrolysis, inactivate the enzyme, and cool it; add activated carbon to the enzymatic hydrolysate, stir it, filter it by suction, concentrate the filtrate, and freeze-dry it to obtain corn protein peptide. The corn protein peptide prepared by this method has a high degree of hydrolysis and improves the utilization rate of corn protein;

[0004] For the prior art, especially in the above-mentioned scheme, generally, raw materials are pulverized by a pulverizing device or a cutting device, and then the raw materials are put into an enzymatic hydrolysis tank and an extraction solution is added for enzymatic hydrolysis, and finally filtered. However, for some raw materials that cannot be powdered, such as meat, vegetable, and fruit raw materials, since the raw materials are soaked in the extraction solution in the enzymatic hydrolysis tank for a long time, the raw materials will inevitably absorb a part of the extraction solution, and a part of the liquid generated by the reaction will remain inside the raw materials. If the raw materials are directly discarded, this part of the liquid cannot enter the subsequent processing process for utilization, resulting in waste of resources. Summary of the Invention

[0005] The purpose of the present invention is to provide a technical solution to squeeze the raw materials soaked in the extraction solution through an extrusion mechanism to separate the liquid from the raw materials, so as to solve the problems in the prior art mentioned in the above background art.

[0006] To achieve the above purpose, the present invention adopts the following technical scheme:

[0007] A high-efficiency extraction process for papain peptides includes the following steps:

[0008] S1. Raw material preparation and pre-treatment: Wash the unripe green papaya and cut it into papaya slices with a thickness of 2 mm - 3 mm to increase the contact area between the papaya slices and the extraction solution, and then perform a short-term high-temperature treatment to inactivate endogenous enzymes to prevent component degradation during the subsequent extraction process;

[0009] S2. Enzymatic hydrolysis: Put the papaya slices after high-temperature treatment into the enzymatic hydrolysis equipment, and add an extraction solution to the enzymatic hydrolysis equipment for hydrolyzing papain. The extraction solution is trypsin, so that small peptides are generated inside the enzymatic hydrolysis equipment. The temperature during enzymatic hydrolysis is controlled at 40°C - 50°C, and the enzymatic hydrolysis time is 2h - 6h;

[0010] S3. Separation and purification: Take out the solid-liquid mixture after enzymatic hydrolysis, and remove the remaining residues through centrifugation to obtain a crude extract. Then, ultrafiltrate the crude extract through an ultrafiltration membrane with a molecular weight ≤ 10 kDa to separate and obtain a high-purity peptide solution;

[0011] S4. Drying: Concentrate and spray-dry the high-purity peptide solution to obtain papain peptide powder;

[0012] Preferably, the enzymatic hydrolysis includes the following steps:

[0013] The enzymatic hydrolysis equipment stirs the raw materials and the extraction solution to make them evenly mixed. After enzymatic hydrolysis, the solid-liquid mixture is discharged through the pipeline under the enzymatic hydrolysis equipment and extruded. The solid residue is extruded from multiple angles, so that the liquid remaining in the solid residue is separated from the solid residue;

[0014] Among them, there is an enzymatic hydrolysis equipment for raw materials. The enzymatic hydrolysis equipment includes an enzymatic hydrolysis mechanism, an extrusion mechanism, and a separation mechanism;

[0015] The enzymatic hydrolysis mechanism is used to soak the raw materials in the extraction solution for enzymatic hydrolysis. The extrusion mechanism is used to extrude the solid residue in the solid-liquid mixture after enzymatic hydrolysis. The separation mechanism is used to separate the extruded solid residue and liquid.

[0016] Preferably, the enzymatic hydrolysis mechanism includes a tank body and a discharge pipe. The discharge pipe is fixedly connected to the central part of the bottom of the tank body. The extrusion mechanism is installed and connected to the discharge pipe. The separation mechanism is arranged below the extrusion mechanism.

[0017] Preferably, the extrusion mechanism includes a collection box, a first roller, a second roller, and a driving component. The collection box is fixedly connected to the bottom of the discharge pipe. The first roller is horizontally and fixedly installed on the inner wall of the collection box. The two second rollers are rotationally connected through the driving component and horizontally movably connected inside the collection box. The driving component can drive the two second rollers to rotate circumferentially and move away from or close to each other, so that the second roller and the first roller can extrude the solid residue.

[0018] Preferably, the separation mechanism includes an orifice plate and a through groove. The orifice plate is fixedly installed obliquely on the inner wall of the collection box. The through groove is arranged between the orifice plate and the collection box and is horizontally arranged. The extruded raw material can move on the inclined orifice plate until it is discharged through the through groove, while the extruded liquid and the original liquid in the tank can be directly discharged through the holes of the orifice plate to achieve solid-liquid separation.

[0019] Preferably, the driving assembly includes a bracket, a chute, a spline shaft, a first motor, a second motor, a gear ring, a gear, a rack and a bearing. The chute is arranged at the top of the collection box. The spline shaft is rotatably connected to the inner wall of the collection box. The first motor is fixedly installed on the surface of the collection box and is connected to one end of the spline shaft. The bearing is rotatably connected to the inside of the bracket. The bearing and the second roller are both key-connected to the surface of the spline shaft. The bracket is slidably connected to the inside of the chute. The rack is fixedly installed on the top of the bracket. The gear ring is rotatably connected to the surface of the discharge pipe and meshes with the rack. The second motor is fixedly installed on the surface of the collection box. The gear is key-connected to the output end of the second motor and meshes with the gear ring.

[0020] The second motor drives the gear ring to rotate reciprocally in a periodic forward and reverse manner, so that the gear ring drives the two brackets to move away from or close to each other through the two racks at the same time, so that the brackets drive the second roller and the first roller to rub the solid residue. At the same time, the first motor can drive the second roller to rotate through the spline shaft, so that the first roller and the second roller squeeze the solid residue, thereby achieving the effect of squeezing out the liquid inside the solid residue.

[0021] Preferably, the enzymatic hydrolysis mechanism further includes a feed pipe, a third motor and a stirring rod. The feed pipe is fixedly communicated with the top of the tank. The third motor is fixedly installed on the top of the tank. The stirring rod is rotatably connected to the top of the inner cavity of the tank and is connected to the output end of the third motor. The third motor drives the stirring rod to rotate inside the tank to stir the raw material and the extraction liquid to make them evenly mixed.

[0022] Preferably, the extrusion mechanism further includes a scraper. The scraper is fixedly installed obliquely on the inner wall of the collection box, and the edge of one side of the scraper is in contact with the surface of the second roller. When the second roller rotates, it can rub against the scraper, so as to scrape off the solid residue adhered to the surface of the second roller through the scraper to clean the second roller.

[0023] Preferably, the driving assembly further includes an avoidance groove. The avoidance groove is arranged on the side of the bracket. One side of the discharge pipe extends into the avoidance groove. The bottom of the gear ring is in contact with the top surface of the bracket, so that the gear ring and the rack can be stably meshed, thereby ensuring the transmission accuracy between the gear ring and the rack.

[0024] Preferably, the thickness of the side of the scraper in contact with the second roller is less than the thickness of the side of the scraper away from the second roller.

[0025] Technical effects and advantages of the present invention: The high-efficiency extraction process of papain peptides proposed by the present invention has the following advantages compared with the prior art:

[0026] 1. In the present invention, an enzymatic hydrolysis device stirs the raw materials and the extraction liquid to make them evenly mixed. After the enzymatic hydrolysis is completed, the solid-liquid mixture is discharged through the pipeline under the enzymatic hydrolysis device and extruded. The solid residue is extruded from multiple angles, so that the liquid remaining in the solid residue is separated from the solid residue, thereby increasing the total amount of the liquid, greatly reducing the content of the residue in the liquid entering the centrifuge, and further enabling the centrifugation stage to be completed quickly, improving the extraction efficiency of papain peptides;

[0027] 2. In the present invention, a driving component drives two second rollers in the extrusion mechanism to approach or move away from each other. At this time, the second rollers can cooperate with the first roller to generate friction with the solid residue, and at the same time, the second rollers themselves can rotate on the spline shaft, and thus can perform multi-directional extrusion on the solid residue on the surface of the first roller, so that the liquid inside the solid residue can be fully separated, in order to improve the final yield and extraction efficiency of papain peptides.

[0028] Other features and advantages of the present invention will be described in the subsequent description, and part of them will be obvious from the description, or understood by implementing the present invention. The objectives and other advantages of the present invention can be achieved and obtained through the structures pointed out in the description and the drawings. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 is a flowchart of the method of the present invention;

[0030] Figure 2 is a schematic diagram of the main structure of the enzymatic hydrolysis device of the present invention;

[0031] Figure 3 is a schematic diagram of the bottom structure of the enzymatic hydrolysis device of the present invention;

[0032] Figure 4 is a schematic diagram of the internal structure of the tank body of the present invention;

[0033] Figure 5 is a schematic diagram of the main structure of the driving component of the present invention;

[0034] Figure 6 is a schematic diagram of the transmission structure of the bracket and the gear of the present invention;

[0035] Figure 7 is a schematic sectional view of the collection box of the present invention;

[0036] Figure 8 This is a schematic diagram of the connection structure between the spline shaft and the bracket of the present invention.

[0037] In the figure: 1. Enzymolysis mechanism; 11. Tank body; 12. Discharge pipe; 13. Feed pipe; 14. Third motor; 15. Stirring rod; 2. Extrusion mechanism; 21. Collection box; 22. First roller; 23. Second roller; 24. Driving assembly; 241. Bracket; 242. Chute; 243. Spline shaft; 244. First motor; 245. Second motor; 246. Gear ring; 247. Gear; 248. Rack; 249. Bearing; 2410. Avoidance groove; 25. Scraper; 3. Separation mechanism; 31. Orifice plate; 32. Through groove. Detailed implementation manners

[0038] Now, the subject matter described herein will be discussed with reference to exemplary embodiments. It should be understood that discussing these embodiments is only to enable those skilled in the art to better understand and thus implement the subject matter described herein. Without departing from the scope of protection of the content of this specification, changes can be made to the functions and arrangements of the elements discussed. Each example can omit, substitute, or add various processes or components as needed. Additionally, the features described in some examples can also be combined in other examples.

[0039] The invention provides a high-efficiency extraction process for papain peptides. Please refer to Figures 1 to 8 , and the extraction process includes the following steps:

[0040] S1. Raw material preparation and pretreatment: After washing the unripe green papaya, cut it into papaya slices with a thickness of 2 mm - 3 mm to increase the contact area between the papaya slices and the extraction solution. Then, perform a short-term high-temperature treatment to inactivate endogenous enzymes and prevent component degradation during the subsequent extraction process.

[0041] S2. Enzymolysis: Put the papaya slices after high-temperature treatment into an enzymolysis device, and add an extraction solution for hydrolyzing papain to the enzymolysis device. The extraction solution is trypsin to generate small-molecule peptides inside the enzymolysis device. The temperature during enzymolysis is controlled at 40°C - 50°C, and the enzymolysis time is 2 h - 6 h. The dosage of the extraction solution and the enzymolysis time need to be determined according to the total amount of papaya slices. Generally, it is based on the extraction solution level being 10 cm higher than the top of the papaya slices. When the amount of papaya slices is small, the enzymolysis time is 2 h; when the amount of papaya slices is large, the enzymolysis time is 6 h.

[0042] S3. Separation and purification: Take out the solid-liquid mixture after enzymolysis, and remove the remaining residues through centrifugation to obtain a crude extract. Then, perform ultrafiltration separation on the crude extract through an ultrafiltration membrane with a molecular weight ≤ 10 kDa to separate and obtain a high-purity peptide solution.

[0043] S4. Drying: Concentrate and spray-dry the high-purity peptide solution to obtain papain peptide powder.

[0044] In this embodiment, papain peptide is prepared from papaya slices. Since it is impossible to obtain powdered raw materials from papaya, the surface area of papaya can be further increased by cutting it into pieces to improve the contact area with the extraction solution, thereby accelerating the subsequent enzymatic hydrolysis rate as much as possible. In this embodiment, papain is inactivated by the steam spraying method, and the spraying duration is 3 minutes. Ensuring that the central temperature of the papaya slices reaches 80 degrees Celsius can inactivate papain.

[0045] It should be noted that although the contact area with the extraction solution can be increased by pulping papaya, water needs to be added when pulping papaya, which will lead to an increase in the total volume of the raw materials, making it impossible to accurately control the dosage of the extraction solution. If no water is added, the viscosity of the papaya pulp will be relatively high, making it difficult to mix evenly with the extraction solution.

[0046] The enzymatic hydrolysis equipment stirs the raw materials and the extraction solution to make them evenly mixed. After enzymatic hydrolysis, the solid-liquid mixture is discharged through the pipeline below the enzymatic hydrolysis equipment and enters the extrusion mechanism 2. The extrusion mechanism 2 below the pipeline extrudes the solid residue in the solid-liquid mixture from multiple angles, so that the liquid remaining in the solid residue is separated from the solid residue.

[0047] Please refer to Figures 2 to 5 and Figure 7 , the enzymatic hydrolysis equipment includes: an enzymatic hydrolysis mechanism 1, an extrusion mechanism 2, and a separation mechanism 3.

[0048] The enzymatic hydrolysis mechanism 1 is used to soak the raw materials in the extraction solution for enzymatic hydrolysis. The extrusion mechanism 2 is used to extrude the solid residue in the solid-liquid mixture after enzymatic hydrolysis. The separation mechanism 3 is used to separate the extruded solid residue and liquid. In this embodiment, the extrusion mechanism 2 can be applied to various sheet-like or block-like raw materials, so as to fully separate the liquid in the raw materials, enabling the liquid to be separated inside the centrifuge to achieve an increase in output.

[0049] The enzymatic hydrolysis mechanism 1 includes a tank body 11 and a discharge pipe 12. The discharge pipe 12 is fixedly connected to the central part of the bottom of the tank body 11. The extrusion mechanism 2 is installed and connected to the discharge pipe 12. The separation mechanism 3 is arranged below the extrusion mechanism 2.

[0050] The extrusion mechanism 2 includes a collection box 21, a first roller 22, a second roller 23, and a drive assembly 24. The collection box 21 is fixedly communicated with the bottom of the discharge pipe 12. The first roller 22 is horizontally and fixedly installed on the inner wall of the collection box 21. The two second rollers 23 are rotationally connected through the drive assembly 24 and horizontally movably connected inside the collection box 21. The drive assembly 24 can drive the two second rollers 23 to rotate circumferentially while moving away from or approaching each other, so that the second roller 23 and the first roller 22 can extrude the solid residue;

[0051] The separation mechanism 3 includes an orifice plate 31 and a through groove 32. The orifice plate 31 is inclined and fixedly installed on the inner wall of the collection box 21. The through groove 32 is arranged between the orifice plate 31 and the collection box 21, and the through groove 32 is horizontally arranged. The extruded raw material can move on the inclined orifice plate 31 until it is discharged through the through groove 32, while the extruded liquid and the original liquid in the tank body 11 can be directly discharged through the holes of the orifice plate 31 to achieve solid-liquid separation.

[0052] The drive assembly 24 includes a bracket 241, a chute 242, a spline shaft 243, a first motor 244, a second motor 245, a gear ring 246, a gear 247, a rack 248, and a bearing 249. The chute 242 is arranged on the top of the collection box 21. The spline shaft 243 is rotationally connected to the inner wall of the collection box 21. The first motor 244 is fixedly installed on the surface of the collection box 21 and connected to one end of the spline shaft 243. The bearing 249 is rotationally connected inside the bracket 241. Both the bearing 249 and the second roller 23 are key-connected to the surface of the spline shaft 243. The bracket 241 is slidably connected inside the chute 242. The rack 248 is fixedly installed on the top of the bracket 241. The gear ring 246 is rotationally connected to the surface of the discharge pipe 12 and meshes with the rack 248. The second motor 245 is fixedly installed on the surface of the collection box 21. The gear 247 is key-connected to the output end of the second motor 245 and meshes with the gear ring 246;

[0053] The second motor 245 drives the gear ring 246 to reciprocate by periodically rotating forward and backward, so that the gear ring 246 can drive the two brackets 241 to move away from or approach each other through the two racks 248 at the same time, so that the bracket 241 drives the second roller 23 and the first roller 22 to rub the solid residue. At the same time, the first motor 244 can drive the second roller 23 to rotate through the spline shaft 243, so that the first roller 22 and the second roller 23 extrude the solid residue, so as to achieve the effect of squeezing out the liquid inside the solid residue.

[0054] It should be noted that since the solid residue is flexible, the second roller 23 and the first roller 22 will not be stuck due to too much solid residue when extruding the solid residue. Therefore, the first motor 244 will not be burned out due to the spline shaft 243 being stuck.

[0055] Please refer to Figures 5 to 8 Figures 5 to 8 , the solid-liquid mixture can enter the interior of the collection box 21 through the discharge pipe 12. At this time, the liquid can smoothly pass through the gap between the second roller 23 and the first roller 22, and be discharged from the interior of the collection box 21 through the orifice plate 31. At this time, the staff can collect the liquid through an additional collection device, while the remaining solid residues in the solid-liquid mixture will stay in the gap between the first roller 22 and the second roller 23. At this time, start the first motor 244 and the second motor 245. The first motor 244 can drive the spline shaft 243 to rotate, and the spline shaft 243 can drive the second roller 23 to rotate on the inner wall of the bracket 241. When the second roller 23 rotates, it will cooperate with the first roller 22 to squeeze the solid residues. The liquid absorbed inside the solid residues will separate from the inside of the solid residues after being squeezed, and thus be discharged through the orifice plate 31. Finally, after the solid residues are squeezed and deformed by the second roller 23 and the first roller 22, they can move along the orifice plate 31 to the through groove 32, and finally be discharged to the outside of the collection box 21 through the through groove 32 for unified collection.

[0056] In the above process, the spline shaft 243 can drive the inner ring of the bearing 249 to rotate synchronously. The outer ring of the bearing 249 is fixedly installed inside the bracket 241. However, the inner ring of the bearing 249 is key-connected to the spline shaft 243. Therefore, the inner ring of the bearing 249 can follow the spline shaft 243 to rotate, and the inner ring of the bearing 249 and the second roller 23 can also translate axially along the spline shaft 243. Thus, it can be seen that:

[0057] After the second motor 245 is started, it can drive the gear 247 to rotate periodically forward and backward (the frequency of periodic forward and reverse rotation is set to 2 Hz). At this time, the gear 247 can drive the gear ring 246 to rotate reversely on the surface of the discharge pipe 12. When the gear ring 246 rotates, it can drive the bracket 241 to move horizontally on the inner wall of the chute 242 through the rack 248. When the gear ring 246 starts to rotate to the point where it is about to change the rotation direction, the bracket 241 just moves from one end of the chute 242 to the other end of the chute 242. At this time, the bracket 241 can drive the second roller 23 to move horizontally back and forth on the surface of the spline shaft 243, so that the second roller 23 can drive the solid residues to continuously rub on the surface of the first roller 22, thereby strengthening the extrusion effect on the solid residues.

[0058] The enzymatic hydrolysis mechanism 1 further includes a feed pipe 13, a third motor 14 and a stirring rod 15. The feed pipe 13 is fixedly communicated with the top of the tank body 11. The third motor 14 is fixedly installed on the top of the tank body 11. The stirring rod 15 is rotatably connected to the top of the inner cavity of the tank body 11 and is connected to the output end of the third motor 14. The third motor 14 drives the stirring rod 15 to rotate inside the tank body 11 to stir the raw materials and the extraction liquid to make them evenly mixed.

[0059] Please refer to Figure 2 and Figure 4 , the third motor 14 can drive the stirring rod 15 to rotate inside the tank body 11, so that the stirring rod 15 can evenly mix the solid raw materials and the extraction liquid, thereby improving the enzymatic hydrolysis efficiency, and the feeding pipe 13 is used for inputting raw materials.

[0060] The extrusion mechanism 2 further includes a scraper 25, the scraper 25 is fixedly installed on the inner wall of the collection box 21 obliquely, and the edge of one side of the scraper 25 is in contact with the surface of the second roller 23. The thickness of the side of the scraper 25 in contact with the second roller 23 is less than the thickness of the side of the scraper 25 away from the second roller 23.

[0061] Please refer to Figure 7 , when the second roller 23 rotates, it can rub against the scraper 25, so that the solid residue adhered to the surface of the second roller 23 can be scraped off by the scraper 25 to clean the second roller 23. And because the thickness of the side of the scraper 25 in contact with the second roller 23 is thinner, this enables one side of the scraper 25 to apply more force on the solid residue. Therefore, the pressure of one side of the scraper 25 on the solid residue can be increased, thus realizing the rapid cleaning of the solid residue.

[0062] The driving assembly 24 further includes an avoidance groove 2410, the avoidance groove 2410 is arranged on the side surface of the bracket 241, one side of the discharge pipe 12 extends into the interior of the avoidance groove 2410, and the bottom of the gear ring 246 is in contact with the top surface of the bracket 241.

[0063] Please refer to Figure 6 , this method enables the gear ring 246 and the rack 248 to be stably engaged, thereby ensuring the transmission accuracy between the gear ring 246 and the rack 248.

[0064] The above describes the embodiments of the present invention, but the present invention is not limited to the above specific embodiments. The above specific embodiments are merely illustrative and not restrictive. Under the inspiration of the present invention, those of ordinary skill in the art can also make many forms, all of which fall within the protection scope of the present invention.

Claims

1. A papain peptide efficient extraction process, characterized in that: The following steps are involved: S1. Raw material preparation and pretreatment: wash the unripe green papaya and cut it into 2mm-3mm papaya slices to increase the contact area between the papaya slices and the extract, and then treat it with high temperature for a short time to inactivate endogenous enzymes to prevent degradation of components during the subsequent extraction process; S2, enzymolysis, putting the papaya slices after high temperature treatment into an enzymolysis device, and adding an extracting solution to the enzymolysis device for hydrolyzing papaya protein, wherein the extracting solution is trypsin, so that small molecule peptides are generated inside the enzymolysis device, the temperature during enzymolysis is controlled at 40°C-50°C, and the enzymolysis time is 2h-6h; S3, separation and purification, taking out the solid-liquid mixture after enzymatic hydrolysis, and removing the remaining residues through a centrifuge to obtain a crude extract, and then ultrafiltration separation of the crude extract, through an ultrafiltration membrane with a molecular weight of ≤10kDa, to obtain a high-purity peptide solution; S4, drying, concentrating and spray-drying the high-purity peptide solution to obtain papaya protein peptide powder.

2. A papain peptide efficient extraction process according to claim 1, characterized in that: The enzymatic hydrolysis comprises the following steps: The enzymatic hydrolysis equipment stirs the raw material and the extract to make them evenly mixed. After the enzymatic hydrolysis is completed, the solid-liquid mixture is discharged and squeezed through the pipeline below the enzymatic hydrolysis equipment. The solid residue is squeezed at multiple angles, so that the liquid remaining in the solid residue is separated from the solid residue; The enzymatic hydrolysis device comprises an enzymatic hydrolysis mechanism (1), an extrusion mechanism (2) and a separation mechanism (3); The enzymatic hydrolysis mechanism (1) is used to immerse the raw material in the extract for enzymatic hydrolysis, the extrusion mechanism (2) is used to extrude the solid residue in the solid-liquid mixture after enzymatic hydrolysis, and the separation mechanism (3) is used to separate the solid residue and the liquid after extrusion.

3. A papain peptide efficient extraction process according to claim 2, characterized in that: The enzymatic hydrolysis mechanism (1) comprises a tank body (11) and a discharge pipe (12), wherein the discharge pipe (12) is fixedly connected to the central part of the bottom of the tank body (11), the extrusion mechanism (2) is installed and connected to the discharge pipe (12), and the separation mechanism (3) is arranged below the extrusion mechanism (2).

4. The efficient extraction process of papain peptide according to claim 3, characterized in that: The extrusion mechanism (2) comprises a collection box (21), a first roller (22), a second roller (23) and a driving assembly (24); the collection box (21) is fixedly connected to the bottom of the discharge pipe (12); the first roller (22) is horizontally fixedly mounted on the inner wall of the collection box (21); the two second rollers (23) are rotationally connected and horizontally movably connected to the inside of the collection box (21) via the driving assembly (24); the driving assembly (24) drives the two second rollers (23) to rotate circumferentially and move away from or closer to each other, so that the second rollers (23) and the first rollers (22) can extrude solid residues.

5. The efficient extraction process of papain peptide according to claim 4, characterized in that: The separation mechanism (3) comprises a perforated plate (31) and a through groove (32); the perforated plate (31) is fixedly installed on the inner wall of the collection box (21) in an inclined manner; the through groove (32) is arranged between the perforated plate (31) and the collection box (21), and the through groove (32) is arranged horizontally; the extruded raw material can move on the inclined perforated plate (31) until it is discharged through the through groove (32); and the extruded liquid and the original liquid in the tank body (11) can be directly discharged through the holes of the perforated plate (31), so as to achieve solid-liquid separation.

6. The efficient extraction process of papain peptide according to claim 5, characterized in that: The driving assembly (24) comprises a bracket (241), a slide groove (242), a spline shaft (243), a first motor (244), a second motor (245), a gear ring (246), a gear (247), a rack (248) and a bearing (249); the slide groove (242) is arranged on the top of the collection box (21); the spline shaft (243) is rotatably connected to the inner wall of the collection box (21); the first motor (244) is fixedly installed on the surface of the collection box (21) and connected to one end of the spline shaft (243); the bearing (249) is rotatably connected to the bracket (241), the bearing (249) and the second roller (23) are both key-connected to the surface of the spline shaft (243), the bracket (241) is slidably connected to the inside of the slide groove (242), the rack (248) is fixedly installed on the top of the bracket (241), the ring gear (246) is rotatably connected to the surface of the discharge pipe (12) and meshes with the rack (248), the second motor (245) is fixedly installed on the surface of the collection box (21), and the gear (247) is key-connected to the output end of the second motor (245) and meshes with the ring gear (246); The second motor (245) drives the gear ring (246) to rotate back and forth in a periodic forward and reverse manner, so that the gear ring (246) drives the two brackets (241) to move away from or approach each other through the two racks (248), so that the brackets (241) drive the second roller (23) and the first roller (22) to rub the solid residue. At the same time, the first motor (244) can drive the second roller (23) to rotate through the spline shaft (243), so that the solid residue is squeezed between the first roller (22) and the second roller (23), thereby achieving the effect of squeezing out the liquid inside the solid residue.

7. The efficient extraction process of papain peptide according to claim 3, characterized in that: The enzymatic hydrolysis mechanism (1) further comprises a feed pipe (13), a third motor (14) and a stirring rod (15); the feed pipe (13) is fixedly connected to the top of the tank body (11); the third motor (14) is fixedly installed on the top of the tank body (11); the stirring rod (15) is rotatably connected to the top of the inner cavity of the tank body (11) and is connected to the output end of the third motor (14); the third motor (14) drives the stirring rod (15) to rotate inside the tank body (11) to stir the raw material and the extract so that the two are evenly mixed.

8. The efficient extraction process of papain peptide according to claim 4, characterized in that: The squeezing mechanism (2) further comprises a scraper (25) which is fixedly installed at an angle on the inner wall of the collecting box (21), and the edge of one side of the scraper (25) contacts the surface of the second roller (23). When the second roller (23) rotates, it can rub against the scraper (25), so that the scraper (25) can scrape off the solid residue adhering to the surface of the second roller (23), thereby cleaning the second roller (23).

9. The efficient extraction process of papain peptide according to claim 6, characterized in that: The driving assembly (24) further comprises an avoidance groove (2410), wherein the avoidance groove (2410) is arranged on the side of the bracket (241), and one side of the discharge pipe (12) extends to the inside of the avoidance groove (2410), and the bottom of the gear ring (246) contacts the top surface of the bracket (241) so that the gear ring (246) and the rack (248) can be stably meshed, thereby ensuring the transmission accuracy between the gear ring (246) and the rack (248).

10. The efficient extraction process of papain peptide according to claim 8, characterized in that: The thickness of the side of the scraper (25) in contact with the second roller (23) is smaller than the thickness of the side of the scraper (25) away from the second roller (23).

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