Biochip, reagent recovery system and reagent recovery method
By designing biochips and their reagent recycling systems, the problem of large amount of reagents in high-throughput gene sequencing is solved, efficient recycling and recycling of reagents is achieved, and the sequencing cost is reduced.
Patent Information
- Application Number
- CN202311796308.7
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2023-12-22
- Publication Date
- 2025-06-24
AI Technical Summary
In the existing high-throughput gene sequencing technology, the amount of sequencing reagents is large, which leads to high sequencing costs and is difficult to reduce.
Design a biochip and its related reagent recovery system. By setting the liquid outlet on the biochip facing upwards, and equipped with a pipetting device and a liquid collection device, efficient recycling and recycling of reagents are achieved.
It effectively reduces the amount of reagents and sequencing costs, improves the utilization rate of reagents, and realizes the recycling and recycling of high-throughput, large-scale biochips.
Smart Images
Figure CN120192838A_ABST
Abstract
Description
Technical Field
[0001] The present application relates to the technical field of biochemical substance analysis, and in particular, to a biochip, a reagent recovery system, and a reagent recovery method. Background Art
[0002] With the continuous development of biotechnology, high-throughput gene sequencing technology has become one of the important tools in modern life science research. High-throughput gene sequencing technology refers to the large-scale parallel sequencing of DNA or RNA to quickly obtain a large amount of genomic or transcriptomic information. The emergence of high-throughput gene sequencing technology has promoted the research progress and wide application in the fields of genomics, transcriptomics, epigenetics, etc.
[0003] Sequencing cost is one of the main factors restricting the wide application of high-throughput gene sequencing technology, and the cost of sequencing reagents accounts for more than 90% of the entire sequencing cost. Therefore, how to reduce the consumption of sequencing reagents has become an important direction in the research of high-throughput sequencing. However, the existing sequencing technology still has a high consumption of sequencing reagents and is difficult to reduce. Summary of the Invention
[0004] In order to solve at least one of the above defects, it is necessary to provide a biochip and a reagent recovery system using the biochip. The reagent recovery system can efficiently recover the reagents flowing out of the biochip and recycle them, effectively reducing the reagent consumption and sequencing cost.
[0005] In addition, the present application also provides a reagent recovery method using the aforementioned reagent recovery system.
[0006] In a first aspect, an embodiment of the present application provides a biochip, which includes: a chip body, a flow channel is provided in the chip body, a liquid outlet communicating with the flow channel is opened on the chip body, and the liquid outlet is arranged upward.
[0007] In some possible embodiments, a hydrophobic coating is provided on the surface of the chip body around the liquid outlet.
[0008] In some possible embodiments, the chip body includes a substrate and a cover plate stacked with the substrate, the flow channel is formed between the substrate and the cover plate, the substrate is configured to fix a biological sample, a liquid inlet communicating with the flow channel is opened on the substrate, and the liquid outlet is opened on the cover plate.
[0009] In some possible embodiments, a plurality of liquid outlets are provided on the cover plate, and the plurality of liquid outlets are located at the edge of the cover plate.
[0010] Second aspect, an embodiment of the present application provides a reagent recovery system, which includes: a biochip, a pipetting device, and a liquid collection device. The biochip is the biochip as described above; the pipetting device is used to aspirate the reagent flowing out through the liquid outlet of the biochip; the liquid collection device is communicated with the pipetting device, and the liquid collection device is used to provide a driving force to discharge the reagent located in the pipetting device into the liquid collection device.
[0011] In some possible embodiments, the pipetting device includes: a pipette assembly and a flexible joint. The pipette assembly includes at least one pipette, and the pipette is used to aspirate the reagent located at the liquid outlet; the flexible joint is provided at one end of the pipette assembly, and the nozzle of the pipette does not exceed the port of the flexible joint.
[0012] In some possible embodiments, the pipette assembly includes multiple pipettes and a sleeve sleeved outside all the pipettes, or the pipette is a capillary tube directly processed and formed inside the pipette assembly.
[0013] In some possible embodiments, the reagent recovery system further includes: a reagent storage device and a cleaning device. The reagent storage device is communicated with the biochip and the liquid collection device respectively; the cleaning device is communicated with the pipetting device, and the cleaning device is used to clean the pipetting device.
[0014] Third aspect, an embodiment of the present application provides a reagent recovery method, which includes:
[0015] Providing the biochip as described above, the reagent enters the flow channel through the liquid inlet and flows out through the liquid outlet;
[0016] Moving the pipetting device above the biochip, and aspirating the reagent flowing out through the liquid outlet by the pipetting device; and
[0017] Connecting the pipetting device with the liquid collection device, and providing a driving force through the liquid collection device to discharge the reagent located in the pipetting device into the liquid collection device.
[0018] In some possible embodiments, after the liquid collection device collects a certain amount of the reagent, the method further includes:
[0019] Providing another driving force through the liquid collection device to transport the reagent located in the liquid collection device to the reagent storage device and then to the biochip.
[0020] The reagent recovery system and reagent recovery method provided by the embodiments of the present application, wherein the liquid outlet of the biochip is arranged upward, simplifying the structure of the biochip installation platform; and there is no need for pipeline connection between the pipetting device and the liquid outlet, and the pipetting device can directly suck and recover the reagent flowing out of the liquid outlet, which can reduce the use of pipelines in the system, greatly save the waste of reagents in the pipelines. At the same time, the reagent recovered by the pipetting device can be transported back to the flow channel of the biochip through the liquid collection device to form a closed-loop system for reagent recycling, thereby improving the reagent utilization rate and reducing the reagent consumption and sequencing cost. The reagent recovery system can realize the reagent recovery and recycling of high-throughput and large biochips. BRIEF DESCRIPTION OF THE DRAWINGS
[0021] In order to more clearly illustrate the technical solutions of the embodiments of the present application, the drawings required to be used in the embodiments of the present application will be briefly introduced below. Obviously, the drawings in the following description are only some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on these drawings.
[0022] Figure 1 It is a schematic structural diagram of a reagent recovery system according to an embodiment of the present application.
[0023] Figure 2 It is a schematic structural diagram of a biochip according to an embodiment of the present application.
[0024] Figure 3 For Figure 2 It is a schematic structural diagram of the formation of reagent droplets on the biochip in
[0025] Figure 4 It is a schematic structural diagram of a biochip according to another embodiment of the present application.
[0026] Figure 5 It is a schematic structural diagram of a biochip according to still another embodiment of the present application.
[0027] Figure 6 It is a schematic structural diagram of a pipetting device according to an embodiment of the present application.
[0028] Figure 7 For Figure 6 It is a schematic structural diagram of the end of the pipetting device in
[0029] Figure 8 It is a schematic structural diagram of reagent suction above the biochip by a pipetting device according to an embodiment of the present application.
[0030] Figure 9 It is a schematic structural diagram of the cooperation between a pipetting device and a liquid collection device according to an embodiment of the present application.
[0031] Figure 10 The structural schematic diagram of the cooperation between the cleaning device and the pipetting device according to an embodiment of the present application.
[0032] Figure 11 The flowchart of the reagent recovery method according to an embodiment of the present application.
[0033] Description of main component symbols
[0034]
[0035]
[0036] The following specific embodiments will further illustrate the present application in conjunction with the above-mentioned drawings. Specific embodiments
[0037] The technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application. Obviously, the described embodiments are only a part of the embodiments of the present application, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present application without creative efforts shall fall within the protection scope of the present application.
[0038] It should be noted that when a component is referred to as "fixed to" or "installed on" another component, it can be directly on the other component or there may also be an intermediate component. When a component is considered to be "disposed on" another component, it can be directly disposed on the other component or there may be an intermediate component at the same time. The term "and / or" used herein includes all and any combinations of one or more of the related listed items.
[0039] Please refer to Figure 1 As shown, the present application provides a reagent recovery system 100 for reagent recovery in biochemical substance analysis. The reagent recovery system 100 includes a biochip 10, a pipetting device 20, and a liquid collection device 30. Among them, the pipetting device 20 is used to aspirate the reagent flowing out through the liquid outlet 16 from the liquid outlet 16 of the biochip 10. The liquid collection device 30 is communicated with the pipetting device 20. The liquid collection device 30 is used to provide a driving force to discharge the reagent in the pipetting device 20 into the liquid collection device 30, so as to realize the recovery and subsequent reuse of the reagent flowing out of the biochip 10.
[0040] Please refer to together Figure 2As shown, the biochip 10 includes a chip body. A flow channel 14 is formed in the chip body, and a liquid outlet 16 communicating with the flow channel 14 is opened on the chip body. The liquid outlet 16 is arranged upward. Here, it is defined that the side of the biochip 10 close to the pipetting device 20 in the reagent recovery system 100 is the upper side, and the side away from the pipetting device 20 is the lower side. The liquid outlet 16 being arranged upward means that the liquid outlet 16 is arranged facing the pipetting device 20. By arranging the liquid outlet 16 upward, it is convenient for the pipetting device 20 to aspirate the reagent at the liquid outlet 16.
[0041] In some embodiments, the chip body includes a stacked base 11 and a cover plate 12, and a sealed flow channel 14 is formed between the base 11 and the cover plate 12. The liquid outlet 16 is opened on the cover plate 12. The base 11 is configured to fix the biological sample. Specifically, a biological functional layer (not shown in the figure) is provided on the surface of the base 11 close to the flow channel 14. The biochip 10 is used for biochemical substance analysis (such as gene sequencing, and the biochip 10 can be a sequencing chip), and the flow channel 14 can provide a place for biochemical substance analysis.
[0042] In some embodiments, a spacer 13 is provided between the base 11 and the cover plate 12. The spacer 13 is used to space the base 11 and the cover plate 12 apart. Specifically, the spacer 13 includes two opposite end faces, one end face is connected to the base 11, and the other end face is connected to the cover plate 12. The base 11, the cover plate 12 and the spacer 13 can form a sealed flow channel 14. In some embodiments, the biochip 10 further has a liquid inlet 15, and the liquid inlet 15 is opened on the base 11.
[0043] As Figure 1 shown, the reagent recovery system 100 further includes a reagent storage device 40. The reagent storage device 40 is used to store the reagents required for biochemical reactions. The liquid inlet 15 is communicated with the reagent storage device 40. The reagent can enter the flow channel 14 through the liquid inlet 15 under the action of an external driving force, and further flow out through the liquid outlet 16 under the action of pressure. The reagent flowing out from the liquid outlet 16 can be aspirated and recovered by the pipetting device 20, and further recycled through the liquid collecting device 30. Different from traditional biochips: for traditional biochips, both the liquid inlet and the liquid outlet are opened on the base, while in the biochip 10 provided by the embodiments of the present application, the liquid inlet 15 is opened on the base 11, and the liquid outlet 16 is opened on the cover plate 12. By arranging the liquid outlet 16 on the cover plate 12, the structure of the biochip installation platform (not shown in the figure) can be simplified, and the liquid outlet 16 is opened above the biochip 10, which is convenient for reagent recovery.
[0044] In some embodiments, the substrate 11 may be a silicon substrate. A large number of recessed microstructures may be formed on the surface of the substrate 11 near the flow channel 14 through a process such as etching. A biofunctional layer is provided on the surface of these recessed microstructures, which can be used to adsorb biological samples and perform biochemical reactions.
[0045] In some embodiments, there may be one liquid inlet 15, which is located at the approximate middle of the substrate 11, so as to uniformly add reagents or biological samples into the flow channel 14, etc., making the liquid pressure in the flow channel 14 more uniform.
[0046] In some embodiments, the cover plate 12 may be a glass sheet, such as a transparent glass sheet, which is convenient for observing the reaction process in the flow channel 14.
[0047] Please refer to Figure 3 As shown, in some embodiments, a hydrophobic coating 17 is formed on the surface of the chip body around the liquid outlet 16. Specifically, the surface of the cover plate 12 facing away from the flow channel 14 (i.e., the upper surface of the cover plate 12) is hydrophobically treated to form the hydrophobic coating 17. The main function of this hydrophobic coating 17 is to promote the reagent flowing out of the liquid outlet 16 to form spherical droplets on the upper surface of the biochip 10, so as to facilitate the aspiration by the pipetting device 20.
[0048] In some embodiments, the liquid outlet 16 is opened at the edge of the cover plate 12, and the setting position of the liquid outlet 16 generally needs to satisfy that the fluid can cover the entire flow channel 14 before flowing out of the liquid outlet 16.
[0049] Please refer to Figure 4 and Figure 5 As shown, in some embodiments, multiple liquid outlets 16 may be provided on the cover plate 12 to improve the efficiency of reagent recovery. In some embodiments, the specific positions of the multiple liquid outlets 16 can be set according to the specific shape of the biochip 10. For example, the shape of the biochip 10 can be circular, fan-shaped, rectangular or square, etc. As Figure 4 shown, when the shape of the biochip 10 is circular, the multiple liquid outlets 16 can be provided on the periphery of the circular biochip 10, and the circular biochip 10 can be rotated, and the fluid enters from the liquid inlet 15 and covers the entire flow channel 14 by centrifugal force. As Figure 5 shown, when the shape of the biochip 10 is rectangular or square, the multiple liquid outlets 16 can be located on the same edge of the biochip 10, and the travel of the pipetting device 20 is shorter, which is convenient for cooperating with the pipetting device 20 for reagent recovery. No matter what shape the biochip 10 (and its flow channel 14) is, the fluid can be injected by positive pressure and fill the flow channel 14.
[0050] In some embodiments, the shape of the liquid outlet 16 can be, but is not limited to, a circular hole, and the aperture does not need to be set too large.
[0051] In some embodiments, the spacer 13 may be in the form of double-sided adhesive and directly bond the substrate 11 and the cover plate 12. It can be understood that an adhesive strip can also be formed by dispensing glue on the substrate 11, and then the cover plate 12 is pressed onto the adhesive strip and cured to form the spacer 13, so as to bond the substrate 11 and the cover plate 12 together to form a sealed flow channel 14. In some embodiments, the thickness of the spacer 13 can be flexibly set according to the specific volume of the required flow channel 14.
[0052] Please refer to Figures 6 to 8 together. The pipetting device 20 includes: a pipette assembly 21 and a flexible joint 22. The pipette assembly 21 includes at least one pipette 23. The pipette 23 is used to aspirate the reagent located at the liquid outlet 16. Specifically, the pipette 23 utilizes the capillary action to aspirate the reagent located at the liquid outlet 16 into the pipette 23. The flexible joint 22 is provided at one end of the pipette assembly 21, and the nozzle of the pipette 23 does not exceed the port of the flexible joint 22. By providing the flexible joint 22 at one end of the pipette assembly 21, it can play a certain protective role on the upper surface of the biochip 10, so as to prevent the pipetting device 20 from colliding rigidly with the cover plate 12 of the biochip 10 during the process of aspirating the reagent and damaging the biochip 10.
[0053] In some embodiments, the pipette assembly 21 includes multiple pipettes 23 and a sleeve 24 sleeved outside all the pipettes 23. By assembling multiple pipettes 23 with smaller inner diameter sizes in the sleeve 24 through a certain process, a multi-micropore pipette can be formed, which can improve the aspiration efficiency of the reagent droplets and thus improve the reagent recovery efficiency. In other embodiments, the multiple pipettes 23 may be capillary tubes directly formed in the pipette assembly 21.
[0054] In some embodiments, a hydrophilic double-sided adhesive layer (not shown in the figure) is provided between the pipette assembly 21 and the flexible joint 22. On the one hand, it can improve the bonding strength between the pipette assembly 21 and the flexible joint 22. On the other hand, the hydrophilic double-sided adhesive layer can enable the reagent droplets at the liquid outlet 16 to be fully aspirated into the pipette 23 and increase the moving speed of the reagent droplets into the pipette 23, thereby accelerating the reagent recovery efficiency.
[0055] Please refer to Figure 9, the liquid collection device 30 includes a liquid collection bottle 31, a bottle cap 32 provided at the bottle mouth of the liquid collection bottle 31, a first pipeline 33 passing through the bottle cap 32 and communicating with the liquid collection bottle 31, and a driving mechanism 36 communicating with the first pipeline 33. An opening 35 communicating with the liquid collection bottle 31 is provided on the bottle cap 32, and the flexible joint 22 of the pipetting device 20 communicates with the opening 35. The driving mechanism 36 provides a driving force (such as a negative pressure driving force) for the inside of the liquid collection bottle 31 through the first pipeline 33, so that the reagent in the pipetting device 20 can be transferred into the liquid collection bottle 31.
[0056] In some embodiments, the opening 35 can be a V-shaped opening, so that the flexible joint 22 can extend into the inside of the V-shaped opening and be in close contact with the side wall of the V-shaped opening, thereby improving the sealing performance inside the liquid collection bottle 31.
[0057] In some embodiments, the driving mechanism 36 can be a driving pump.
[0058] Please refer to again Figure 1 , the reagent storage device 40 of the reagent recovery system 100 is respectively communicated with the liquid inlet 15 and the liquid collection device 30. The reagent storage device 40 can be used to store fluids such as biological samples, reagents required for biochemical reactions, or cleaning solutions. The reagent storage device 40 can load samples or reagents and other fluids into the flow channel 14 of the biochip 10 through the liquid inlet 15. In addition, a second pipeline 34 is also provided through the bottle cap 32, and the liquid collection device 30 and the reagent storage device 40 can be communicated through the second pipeline 34. When the reagent collected in the liquid collection device 30 reaches a certain amount, the driving mechanism 36 will be started to provide another driving force (such as a positive pressure driving force), so that the reagent located in the liquid collection device 30 enters the reagent storage device 40, and then is loaded into the biochip 10 through the reagent storage device 40, forming a closed-loop system, thereby completing the recovery and recycling of the reagent, improving the reagent utilization rate, reducing the reagent consumption and sequencing cost.
[0059] Please refer to again Figure 1 , and refer to in combination Figure 10 , the reagent recovery system 100 further includes: a cleaning device 50, the cleaning device 50 is communicated with the pipetting device 20, and the cleaning device 50 is used to clean the pipetting device 20. When a reagent collection is completed, due to the residue of the previous reagent on the inner wall of the pipette 23, the next reagent cannot be directly collected, and the inner wall of the tube needs to be cleaned. Therefore, the cleaning device 50 can be used to clean the pipetting device 20.
[0060] In some embodiments, the cleaning device 50 includes a cleaning reagent bottle 51, a driving mechanism 52, and a reversing valve 53 that are connected in sequence. One inlet of the reversing valve 53 can be connected to the driving mechanism 52, and the other inlet can be connected to the atmosphere. The outlet of the reversing valve 53 can be connected to the pipetting device 20. By controlling the driving mechanism 52, the reagent in the cleaning reagent bottle 51 can enter the pipetting device 20 via the reversing valve 53 to clean the pipetting device 20. After cleaning, the cleaning liquid in the pipetting device 20 is emptied, and the recovery of the next reagent is performed.
[0061] Please refer to Figure 11 As shown, the embodiment of the present application also provides a method for reagent recovery using the aforementioned reagent recovery system 100. The method includes:
[0062] Step S1, providing the biochip 10 as described above. The reagent enters the flow channel 14 from the liquid inlet 15 and flows out from the liquid outlet 16.
[0063] Please refer to in combination with Figure 1 and Figure 8 As shown, when the biological reagent enters the flow channel 14 of the biochip 10 from the bottom liquid inlet 15 of the biochip 10, it flows towards the liquid outlet 16 on the cover plate 12 under the push of pressure, and then flows out from the liquid outlet 16. Since the surface of the cover plate 12 around the liquid outlet 16 is hydrophobic-treated and the aperture of the liquid outlet 16 is small, spherical reagent droplets will be formed at the liquid outlet 16 on the upper surface of the biochip 10 under the action of molecular force.
[0064] Step S2, moving the pipetting device 20 above the biochip 10, and sucking the reagent flowing out from the liquid outlet 16 through the pipetting device 20.
[0065] Please refer to in combination with 1 and Figure 8 As shown, move the pipetting device 20 above the spherical reagent droplet, and then contact the reagent droplet at a certain speed. Since the aperture of the pipette 23 in the pipetting device 20 is small, the reagent droplet is sucked by its capillary action, and the reagent droplet can be sucked clean and fully.
[0066] Step S3, connecting the pipetting device 20 to the liquid collection device 30, and providing a driving force through the liquid collection device 30 to discharge the reagent located in the pipetting device 20 into the liquid collection device 30.
[0067] Please refer to in combination with Figure 1 and Figure 9As shown, after the pipetting device 20 picks up the reagent droplets, the pipetting device 20 is moved above the liquid collection device 30 and slowly moved downward so that the flexible joint 22 closely fits with the V-shaped opening hole at the mouth of the liquid collection bottle 31 to form a seal. Then, the pipetting device 20 is connected to the atmosphere, and the driving mechanism 36 is activated to create a negative pressure inside the liquid collection bottle 31. Under the action of the atmospheric pressure, the reagent in the pipette 23 is sucked into the liquid collection bottle 31, thus completing the first collection of the reagent.
[0068] In some embodiments, after the liquid collection device 30 collects a certain amount of the reagent, the method further includes:
[0069] Providing another driving force through the liquid collection device 30 to transport the reagent located in the liquid collection device 30 to the biochip 10. Specifically, the liquid collection device 30 is connected to the reagent storage device 40. The driving force can be provided through the liquid collection device 30 to transport the reagent collected in the liquid collection device 30 to the reagent storage device 40, and then further loaded into the biochip 10 to achieve the recycling of the reagent.
[0070] In some embodiments, after the pipetting device 20 recovers one reagent, the method further includes:
[0071] Connecting the pipetting device 20 to the cleaning device 50 and cleaning the pipetting device 20 through the cleaning device 50. Specifically, by controlling the driving mechanism 52, the reagent in the cleaning reagent bottle 51 can enter the pipetting device 20 via the reversing valve 53 to clean the pipetting device 20. After cleaning, the cleaning liquid in the pipetting device 20 is emptied for the recovery of the next reagent. Among them, the cleaning step can be carried out between the collections of two reagents, or the pipetting device 20 can be cleaned at the end of the reagent recovery.
[0072] The reagent recovery system 100 and the reagent recovery method provided by the embodiments of the present application. Among them, the liquid outlet 16 of the biochip 10 is located on the cover plate 12, which simplifies the structure of the biochip installation platform; and there is no need for pipeline connection between the pipetting device 20 and the liquid outlet 16. The pipetting device 20 can directly suck and recover the reagent flowing out of the liquid outlet 16, which can reduce the use of pipelines in the system, greatly save the waste of reagents in the pipelines. At the same time, the reagent recovered by the pipetting device 20 can be transported back to the flow channel 14 of the biochip 10 through the liquid collection device 30 to form a closed-loop system for reagent recycling, thereby improving the reagent utilization rate and reducing the reagent dosage and sequencing cost. The reagent recovery system 100 can achieve the recycling of reagents for high-throughput and large biochips.
[0073] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application and not to limit them. Although the present application has been described in detail with reference to the preferred embodiments, those of ordinary skill in the art should understand that the technical solutions of the present application can be modified or equivalently replaced without departing from the spirit and scope of the technical solutions of the present application.
Claims
1. A biochip, characterized in that, Comprising: A chip body, wherein a flow channel is provided in the chip body, a liquid outlet communicating with the flow channel is formed on the chip body, and the liquid outlet is arranged upward.
2. The biochip according to claim 1, wherein A hydrophobic coating is provided on the surface of the chip body around the liquid outlet.
3. The biochip according to claim 1, wherein, The chip body includes a substrate and a cover plate stacked with the substrate, and the flow channel is formed between the substrate and the cover plate. The substrate is configured to fix a biological sample, a liquid inlet communicating with the flow channel is formed on the substrate, and the liquid outlet is formed on the cover plate.
4. The biochip according to claim 3, characterized in that, A plurality of liquid outlets are provided on the cover plate, and the plurality of liquid outlets are located at the edge of the cover plate.
5. A reagent recovery system, characterized in that, Comprising: A biochip, which is the biochip according to any one of claims 1 to 4; A pipetting device for sucking a reagent flowing out through the liquid outlet of the biochip from the liquid outlet; And A liquid collection device communicating with the pipetting device, and the liquid collection device is used to provide a driving force to discharge the reagent in the pipetting device into the liquid collection device.
6. The reagent recovery system according to claim 5, wherein, The pipetting device includes: A pipette assembly, the pipette assembly includes at least one pipette for sucking the reagent at the liquid outlet; and A flexible joint provided at one end of the pipette assembly, and the pipe orifice of the pipette does not exceed the port of the flexible joint.
7. The reagent recovery system according to claim 6, wherein The pipette assembly includes a plurality of the pipettes and a sleeve sleeved outside all the pipettes, or the pipette is a capillary tube directly formed in the pipette assembly.
8. The reagent recovery system according to claim 5, wherein Further comprising: A reagent storage device communicating with the biochip and the liquid collection device respectively; And A cleaning device communicating with the pipetting device, and the cleaning device is used to clean the pipetting device.
9. A reagent recovery method, characterized in that, Comprising: Providing the biochip according to any one of claims 1 to 4, the reagent enters the flow channel through the liquid inlet and flows out through the liquid outlet; Moving the pipetting device above the biochip and sucking the reagent flowing out through the liquid outlet by the pipetting device; And Connecting the pipetting device with the liquid collection device and providing a driving force through the liquid collection device to discharge the reagent in the pipetting device into the liquid collection device.
10. The reagent recovery method according to claim 9, wherein After the liquid collection device collects a certain amount of the reagent, the method further includes: Providing another driving force through the liquid collection device to transport the reagent in the liquid collection device to the reagent storage device and then to the biochip.