Liquid distribution assembly and sequencing device

Abstract

The invention discloses a liquid distribution assembly and a sequencing device, the liquid distribution assembly is used for distributing liquid to a sample carrier, and the sample carrier is provided with a reaction surface which is open towards the liquid distribution assembly. The liquid distribution assembly comprises a liquid storage part, a liquid guide part and a driver. The liquid storage part comprises a liquid storage cavity for storing reaction liquid; the liquid guide part is communicated with the liquid storage cavity, is movably arranged relative to the reaction surface and is configured to guide the reaction liquid to the reaction surface after obtaining the reaction liquid in the liquid storage cavity, the liquid guide part is provided with a coating tool bit facing the reaction surface, the coating tool bit comprises a liquid outlet, and the size of the liquid outlet in the first direction is larger than the size of the liquid outlet in the second direction which is not parallel to the first direction; the first direction and the second direction are parallel to the reaction surface. And the driver is communicated with the liquid storage cavity so as to drive the reaction liquid in the liquid storage cavity to the liquid guide part. According to the scheme, the reaction liquid is directly guided to the reaction surface, and the area, used for reaction, of the reaction surface can be well infiltrated by the reaction liquid by adopting a relatively small amount of the reaction liquid, so that the dosage of the reaction liquid is reduced.

Description

Technical Field

[0001] The present invention relates to the field of biochemistry, and particularly to a liquid distribution component and a sequencing device. Background Art

[0002] The sample carrier includes a reaction surface, and the corresponding biochemical reaction is completed by wetting the reaction surface with the reaction liquid. For an open sample carrier, the prior art soaks the sample carrier in a plurality of containers filled with the reaction liquid in sequence to complete the biochemical reaction. In this solution, only a small part of the reaction liquid in the container is used for the reaction. However, in order to completely immerse the reaction surface in the reaction liquid, more reaction liquid needs to be injected into the container, resulting in a large amount of reaction liquid consumption. Summary of the Invention

[0003] The main object of the present invention is to provide a liquid distribution component and a sequencing device, which can reduce the consumption of the reaction liquid.

[0004] To achieve the above object, the present invention provides a liquid distribution component for distributing liquid to a sample carrier, the sample carrier having a reaction surface open towards the liquid distribution component, the liquid distribution component comprising:

[0005] A liquid storage part including a liquid storage cavity for storing the reaction liquid;

[0006] A liquid guiding part communicating with the liquid storage cavity and movably arranged opposite to the reaction surface, configured to obtain the reaction liquid in the liquid storage cavity and then guide the reaction liquid to the reaction surface, the liquid guiding part having a coating cutter head facing the reaction surface, the coating cutter head including a liquid outlet, the size of the liquid outlet in a first direction being greater than the size in a second direction not parallel to the first direction, both the first direction and the second direction being parallel to the reaction surface; and,

[0007] A driver communicating with the liquid storage cavity to drive the reaction liquid in the liquid storage cavity to the liquid guiding part.

[0008] In some embodiments, the first direction is perpendicular to the second direction, the reaction surface includes a reaction site array arranged along the first direction and the second direction, and the size of the liquid outlet in the first direction matches the size of the reaction site array in the first direction.

[0009] In some embodiments, the liquid storage cavity includes a plurality of cavities for separately storing the reaction liquid, and the driver is configured to separately guide the reaction liquid in each cavity to the liquid guiding part.

[0010] In some embodiments, the driver includes a driving pump and a valve assembly connected between the driving pump and the liquid storage chamber; the valve assembly has a first state of selectively connecting the plurality of cavities to the driving pump, and a second state of connecting the driving pump to the liquid guide portion;

[0011] or,

[0012] The driver comprises a plurality of driving units, each of which is connected to each of the cavities in a one-to-one correspondence, so that each of the driving units independently guides the reaction liquid in the corresponding cavity to the liquid guide portion;

[0013] or,

[0014] The liquid guiding portion includes a plurality of liquid guiding monomers, each of which is connected to each of the cavities in a one-to-one manner, and each of the liquid guiding monomers is configured to obtain the reaction liquid and then guide the reaction liquid to the reaction surface. The driver includes a plurality of driving monomers, and each of the driving monomers is connected between each of the cavities and each of the liquid guiding monomers in a one-to-one manner.

[0015] In some embodiments, the valve assembly is a rotary valve.

[0016] In some embodiments, the liquid distributing assembly further includes a cleaning part and a driving pump, wherein the driving pump is connected to the cleaning part and introduces gas into the cleaning part, and the cleaning part has an outlet for discharging the gas to the reaction surface to clean the reaction surface.

[0017] In some embodiments, the cleaning portion is connected to the liquid guiding portion and is fixedly arranged relative to the liquid guiding portion, the liquid outlet is arranged adjacent to the gas outlet, and the gas outlet is located at the front side of the liquid outlet along the movement direction relative to the reaction surface.

[0018] In some embodiments, the gas outlet is opened on a side of the cleaning portion away from the liquid guiding portion, and an air flow direction of the gas outlet is not perpendicular to the reaction surface.

[0019] In some embodiments, the liquid storage portion includes at least one liquid storage cavity for storing a viscous reagent, and the liquid guiding portion also includes a scraper disposed adjacent to the liquid outlet, and the scraper is used to spread the viscous reagent flowing out of the liquid outlet on the reaction surface.

[0020] In some embodiments, the liquid guiding portion is provided with a liquid inlet channel and a liquid guiding slit, one end of the liquid inlet channel is connected to the liquid storage cavity, and the other end is connected to the liquid guiding slit, and the liquid guiding slit forms the liquid outlet on the end surface of the liquid guiding portion close to the reaction surface.

[0021] In some embodiments, the liquid guiding part includes a first block and a second block connected to the first block. The first block is provided with the liquid inlet channel therein. A first liquid guiding groove communicating with the liquid inlet channel is provided on the wall surface of the first block facing the second block. The first liquid guiding groove penetrates through the first block along a third direction, and the third direction is perpendicular. The second block covers the notch of the first liquid guiding groove facing the second block, and the notch of the first liquid guiding groove penetrating through the first block along the third direction forms the liquid guiding slit.

[0022] In some embodiments, the scraping member includes a scraping protrusion provided at the edge of the liquid outlet, and the scraping protrusion is located at the rear side of the liquid outlet along the moving direction of the liquid outlet relative to the reaction surface.

[0023] An embodiment of the second aspect of the present application further provides a sequencing device, including:

[0024] The liquid distribution assembly according to any one of the above; and,

[0025] A stage for supporting the sample carrier, and the reaction surface of the sample carrier has sites for supporting the nucleic acid library.

[0026] Compared with the prior art, the beneficial effects of the present invention are:

[0027] In the technical solution of the present invention, the liquid distribution assembly includes a liquid storage part, a liquid guiding part and a driver. The driver guides the reaction liquid in the liquid storage cavity of the liquid storage part to the liquid guiding part, and the reaction liquid guided to the liquid guiding part is finally guided to the reaction surface of the sample carrier. Compared with the reaction method of directly immersing the sample carrier in a container filled with the reaction liquid, firstly, in this solution, the reaction liquid is directly guided to the reaction surface, so that a relatively small amount of reaction liquid can be used to well wet the area of the reaction surface for reaction, reducing the amount of reaction liquid used. Secondly, this solution does not require multiple containers for accommodating the sample carrier, so that the volume of the liquid storage cavity for containing the reaction liquid can be reduced, reducing the occupied space of the liquid distribution assembly. Thirdly, in the existing reaction method of immersing the sample carrier in a container filled with the reaction liquid, a manipulator is required to frequently transfer the sample carrier to different containers, while in this solution, there is no need for the above process of transferring the sample carrier during the reaction stage, the operation difficulty is small, and the reaction efficiency is high. Fourthly, in this solution, the reaction liquid is guided to the reaction surface. Compared with the reaction method of immersing the sample carrier in the reaction liquid, the reaction liquids will not cross-infect, improving the reaction accuracy. Fifthly, compared with the method of guiding the reaction liquid to the substrate and then the substrate guiding the reaction liquid to the reaction surface, this solution directly guides the reaction liquid to the reaction surface, and the reaction efficiency is higher. Description of the Drawings

[0028] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the accompanying drawings required in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, without creative efforts, other drawings can be obtained based on the structures shown in these drawings.

[0029] Figure 1 Schematic diagram of the structure of the liquid distribution component, sample carrier, reaction liquid, and stage combination in the first embodiment of the present invention;

[0030] Figure 2 Schematic diagram of the structure of the liquid distribution component, sample carrier, reaction liquid, and stage combination in the second embodiment of the present invention;

[0031] Figure 3 Schematic diagram of the structure of the liquid distribution component, sample carrier, reaction liquid, and stage combination in the third embodiment of the present invention;

[0032] Figure 4 Schematic diagram of the structure of the liquid distribution component, sample carrier, reaction liquid, and stage combination in the fourth embodiment of the present invention;

[0033] Figure 5 Schematic diagram of the structure of the liquid distribution component, sample carrier, reaction liquid, and stage combination in the fifth embodiment of the present invention;

[0034] Figure 6 Schematic diagram of the structure of the liquid distribution component, sample carrier, reaction liquid, and stage combination in the sixth embodiment of the present invention;

[0035] Figure 7 Schematic diagram of the structure of the liquid guiding part, sample carrier, reaction liquid, and stage combination in the seventh embodiment of the present invention;

[0036] Figure 8 Schematic diagram of the structure of the liquid guiding part, cleaning part, sample carrier, reaction liquid, and stage combination in the eighth embodiment of the present invention;

[0037] Figure 9 For Figure 8 Partial enlarged view at position A in;

[0038] Figure 10 Stereoscopic diagram of the liquid guiding part in the first embodiment of the present invention;

[0039] Figure 11 Cross-sectional view of the liquid guiding part in the first embodiment of the present invention;

[0040] Figure 12 Figure 11 Partial enlarged view at position B in;

[0041] Figure 13 Explosion schematic diagram of the liquid guiding part in the first embodiment of the present invention;

[0042] Figure 14 is Figure 13 Partial enlarged schematic diagram at position C in

[0043] Explanation of the reference numerals in the drawings:

[0044] Liquid distribution assembly 10;

[0045] Liquid guiding part 100; first block 110; first hole 111; second hole 112; third hole 113; first liquid guiding groove 114; second liquid guiding groove 115; liquid outlet 116; first inclined side 117; atomizing component 118; second block 120; second inclined side 121; liquid scraping protrusion 130; liquid guiding monomer 140;

[0046] Liquid storage part 200; liquid storage cavity 210; cavity 211; flow dividing disk 220; first diversion port 221; second diversion port 222;

[0047] Driver 300; driving monomer 310;

[0048] Driving pump 400;

[0049] First air valve 510; second air valve 520;

[0050] Cleaning part 600; air outlet channel 610; first end 611; second end 612;

[0051] Reaction liquid 700;

[0052] Gas 800;

[0053] Sample carrier 900; reaction surface 910;

[0054] Carrier table 20;

[0055] First direction Y; second direction X; third direction Z.

[0056] The realization, functional features and advantages of the object of the present invention will be further described with reference to the embodiments and the accompanying drawings. Specific embodiments

[0057] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. All other embodiments obtained by those of ordinary skill in the art based on the embodiments of the present invention without creative efforts shall fall within the protection scope of the present invention.

[0058] The sample carrier includes a reaction surface, and the corresponding biochemical reaction is completed by wetting the reaction surface with a reaction solution. For an open sample carrier, the prior art soaks the sample carrier in multiple containers filled with reaction solutions in sequence to complete the biochemical reaction. In this solution, on the one hand, only a small part of the reaction solution in the container is used for the reaction. However, in order to completely immerse the reaction surface in the reaction solution, a large amount of reaction solution needs to be injected into the container, resulting in a large consumption of the reaction solution. On the other hand, since multiple containers for holding different types of reaction solutions need to be arranged, and each container needs to be able to accommodate the sample carrier, the volume of each container is large, so that the occupied space of each container is large. On the third hand, when the sample carrier needs to react in different reaction solutions, a manipulator is required to frequently transfer the sample carrier, with a large operation difficulty and low reaction efficiency. On the fourth hand, when the sample carrier is transferred between different containers, cross-infection of the reaction solutions in different containers will occur, resulting in low reaction accuracy.

[0059] In view of this, referring to Figures 1 - 14 , this embodiment provides a liquid distribution assembly 10, which includes a liquid storage part 200, a liquid guiding part 100 and a driver 300. The liquid distribution assembly 10 is used to directly guide the reaction solution 700 to the reaction surface 910 of the sample carrier 900, and the reaction surface 910 has sites for supporting nucleic acid libraries.

[0060] Specifically referring to Figures 1 - 3 , the liquid storage part 200 includes a liquid storage cavity 210 for storing the reaction solution 700. The liquid storage cavity 210 is a container for storing the reaction solution 700. One type of reaction solution 700 or multiple types of reaction solutions 700 can be stored in the liquid storage cavity 210. When only one type of reaction solution 700 is stored in the liquid storage cavity 210, in order to enable the reaction surface 910 to react with multiple reaction solutions 700, the liquid distribution assembly 10 can include multiple liquid storage cavities 210, and different types of reaction solutions 700 are stored in the multiple liquid storage cavities 210 respectively. When multiple types of reaction solutions 700 are stored in the liquid storage cavity 210, the liquid storage cavity 210 can include multiple cavities 211, each cavity 211 is independently arranged, and at least two cavities 211 store different types of reaction solutions 700. In some embodiments, the liquid storage cavity 210 can also include multiple cavities 211, and each cavity 211 stores the same type of reaction solution 700 respectively, and the liquid distribution assembly 10 includes multiple liquid storage cavities 210, and different types of reaction solutions 700 are stored in each liquid storage cavity 210. For the convenience of description, the following takes the liquid storage cavity 210 including multiple cavities 211, and each cavity 211 stores different types of reaction solutions 700 as an example for illustration.

[0061] The reaction liquid 700 is defined as follows: Any liquid required by the liquid distribution component 10 during operation is referred to as the reaction liquid 700. During operation, the liquid distribution component 10 not only requires the liquid for reaction with the reaction surface 910 of the sample carrier 900, but may also require the liquid for cleaning the pipeline of the liquid distribution component 10 or the reaction surface 910 of the sample carrier 900. Whether it is the liquid for reaction with the reaction surface 910 or the liquid for cleaning the pipeline of the liquid distribution component 10 or the reaction surface 910 of the sample carrier 900, it is referred to as the reaction liquid 700 in this article.

[0062] See Figure 1 and Figures 10 - 14 , the liquid guiding part 100 is communicated with the liquid storage cavity 210, and the liquid guiding part 100 is configured to direct the reaction liquid 700 in the liquid storage cavity 210 to the reaction surface 910 of the sample carrier 900 after obtaining the reaction liquid 700. That is to say, in the liquid distribution component 10 of this embodiment, the liquid in the liquid storage cavity 210 is directly directed to the reaction surface 910 of the sample carrier 900 by using the liquid guiding component, rather than immersing the sample carrier 900 in a container filled with the reaction liquid 700 in the prior art. The liquid guiding structure and liquid guiding method of the liquid guiding part 100 depend on actual requirements, and details are described below. It should be noted that any device that can obtain the reaction liquid 700 in the liquid storage cavity 210, and can direct the obtained reaction liquid 700 to the reaction surface 910 of the sample carrier 900 and enable the reaction liquid 700 to react well with the reaction surface 910 is referred to as the liquid guiding part 100 in this embodiment.

[0063] The liquid guiding part 100 is movably arranged relative to the reaction surface 910 of the sample carrier 900. Specifically, it can be that the sample carrier 900 does not move and the liquid guiding part 100 moves; or the liquid guiding part 100 does not move and the sample carrier 900 moves. Hereinafter, the case where the sample carrier 900 does not move and the liquid guiding part 100 moves is taken as an example for illustration. The liquid guiding part 100 has a coating knife head facing the reaction surface 910, and the coating knife head includes a liquid outlet 116. The size of the liquid outlet 116 in the first direction Y is greater than the size in the second direction X that is not parallel to the first direction Y. Both the first direction Y and the second direction X are parallel to the reaction surface 910. In other words, the liquid outlet 116 is a long strip-shaped liquid guiding slit. Specifically, the first direction Y can be perpendicular to the second direction X, and the liquid outlet 116 is rectangular. The long side direction of the liquid outlet 116 is parallel to the first direction Y, and the short side direction of the liquid outlet 116 is parallel to the second direction X. The liquid guiding part 100 moves relative to the reaction surface 910 along the second direction X relative to the reaction surface 910, so as to lay the reaction liquid on the reaction surface 910.

[0064] In some embodiments, the first direction Y is perpendicular to the second direction X. The reaction surface 910 includes an array of reaction sites arranged along the first direction Y and the second direction X. The size of the liquid outlet 116 along the first direction Y matches the size of the array of reaction sites along the first direction Y. This enables the reaction liquid to be well laid on the reaction surface 910 after the liquid guiding portion 100 moves relative to the reaction surface 910 along the second direction X. Specifically, the size of the liquid outlet 116 along the first direction Y can be equal to or slightly larger than the size of the array of reaction sites along the first direction Y, so that the reaction liquid can cover each array of reaction sites.

[0065] Referring to Figure 1 , the driver 300 is in communication with the liquid storage chamber 210 to drive the reaction liquid 700 in the liquid storage chamber 210 to the liquid guiding portion 100. That is, the driver 300 can generate a driving force for driving the movement of the reaction liquid 700 by changing the air pressure. On the one hand, this driving force can guide the reaction liquid 700 in the liquid storage chamber 210 to the liquid guiding portion 100, and on the other hand, it can also guide the reaction liquid 700 in the liquid guiding portion 100 to the reaction surface 910 of the sample carrier 900. The driver 300 can be a single component capable of changing the air pressure, or a combination of multiple components capable of changing the air pressure. The specific structure of the driver 300 depends on the actual requirements, as long as it can generate a driving force for driving the movement of the reaction liquid 700. Exemplarily, referring to Figure 1 , in some embodiments, the driver 300 can be a cylindrical plug, which generates positive pressure or negative pressure through the movement of an internal piston to drive the movement of the reaction liquid 700. In other embodiments, the driver 300 can also be an air pump, and the air pump drives the movement of the reaction liquid 700 by generating compressed air and releasing the compressed air.

[0066] In the liquid distribution component 10 of this embodiment, compared with the reaction method in the prior art of directly immersing the sample carrier 900 into the container filled with the reaction liquid 700, in the first aspect, in this solution, the reaction liquid 700 is directly guided to the reaction surface 910, so that a relatively small amount of the reaction liquid 700 can well wet the area of the reaction surface 910 for reaction, reducing the consumption of the reaction liquid 700. In the second aspect, this solution does not require multiple containers for accommodating the sample carrier 900, enabling the volume of the liquid storage cavity 210 filled with the reaction liquid 700 to be reduced, and reducing the occupied space of the liquid distribution component 10. In the third aspect, in the existing reaction method of immersing the sample carrier 900 in the container filled with the reaction liquid 700, it is necessary to use a manipulator to frequently transfer the sample carrier 900 to different containers, while in this solution, there is no need for the above process of transferring the sample carrier 900 during the reaction stage, with relatively low operation difficulty and high reaction efficiency. In the fourth aspect, in this solution, the reaction liquid 700 is guided to the reaction surface 910, and compared with the reaction method of immersing the sample carrier 900 in the reaction liquid 700, the reaction liquids 700 will not cross-infect, improving the reaction accuracy. In the fifth aspect, compared with the method of guiding the reaction liquid 700 to the substrate and then the substrate guiding the reaction liquid 700 to the reaction surface 910, in this solution, the reaction liquid 700 is directly guided to the reaction surface 910, with higher reaction efficiency.

[0067] The reaction surface 910 of the sample carrier 900 needs to react with various types of reaction liquids 700, and each reaction liquid 700 needs to be guided to the reaction surface 910 in a specific order. To achieve the above reaction process, refer to Figures 2 - 4, in some embodiments, the liquid storage chamber 210 includes a plurality of cavities 211 that separately store the reaction liquid 700. Different types of reaction liquid 700 can be separately stored in each cavity 211, and the driver 300 is configured to separately direct the reaction liquid 700 in each cavity 211 to the liquid guiding portion 100. That is, the driver 300 can first direct the first type of reaction liquid 700 in one of the cavities 211 to the reaction surface 910. After the first type of reaction liquid 700 is directed to the reaction surface 910 and the reaction is completed, the driver 300 then directs the second type of reaction liquid 700 in another cavity 211 to the reaction surface 910, and so on. In this way, all types of reaction liquid 700 can be sequentially directed to the reaction surface 910 (before switching the reaction liquid 700, the reaction surface 910 can be cleaned first), thereby completing the reaction process of the sample carrier 900. In this solution, multiple types of reaction liquid 700 can be directed to the reaction surface 910 simultaneously, improving the reaction efficiency. Of course, in other embodiments, multiple liquid storage chambers 210 can also be provided, and each liquid storage chamber 210 separately stores different types of reaction liquid 700, so that only one type of reaction liquid 700 can be directed from one liquid storage chamber 210 to the reaction surface 910. In another embodiment, multiple cavities 211 can also be provided in the liquid storage chamber 210, and the reaction liquid 700 stored in each cavity 211 is the same or partially the same. The driver 300 can separately direct the reaction liquid 700 in each cavity 211 to the liquid guiding portion 100. In this solution, the same type of reaction liquid 700 is distributed in different cavities 211. On the one hand, quantitative distribution of the reaction liquid 700 can be achieved, and on the other hand, the remaining amount of the reaction liquid 700 in each cavity 211 can be observed to accurately understand the remaining amount of this type of reaction liquid 700 and the total consumption amount of this type of reaction liquid 700 (for example, when there are ten cavities 211 in the liquid storage chamber 210 and the reaction liquid 700 in five of the cavities 211 is completely exported, it can be concluded that half of this type of reaction liquid 700 has been consumed and the remaining amount is half of the original). In this solution, when different types of reaction liquid 700 need to be distributed to the reaction surface 910, multiple liquid storage chambers 210 can be provided. The types of reaction liquid 700 in each liquid storage chamber 210 are different, and multiple cavities 211 are provided in each liquid storage chamber 210. The types of reaction liquid 700 provided in each cavity 211 are the same or partially the same. The driver 300 can separately direct the reaction liquid 700 in a single cavity 211 in each liquid storage chamber 210 to the reaction surface 910.

[0068] The structures for separately discharging the reaction liquid 700 in each cavity 211 in the liquid storage chamber 210 are diverse. Refer to Figure 2, in some embodiments, the driver 300 includes a driving pump and a valve assembly connected between the driving pump and the liquid storage chamber 210; the valve assembly has a first state in which multiple cavities are selectively communicated with the driving pump respectively, and a second state in which the driving pump is communicated with the liquid guiding part 100. Specifically, the liquid storage part 200 includes a flow dividing part (the valve assembly includes a flow dividing part), and the flow dividing part has at least a first state and a second state. In the first state, the flow dividing part communicates the driver 300 and any one of the cavities 211, so that the driver 300 extracts the reaction liquid 700. In the second state, the flow dividing part communicates the driver 300 and the liquid guiding part 100, so that the driver 300 guides the extracted reaction liquid 700 to the liquid guiding part 100. In the first state of the flow dividing part, when it is necessary to export the reaction liquid 700 in a certain cavity 211, the flow dividing part correspondingly connects the cavity 211 with the driver 300, thereby achieving the purpose that the driver 300 can separately guide the reaction liquid 700 in each cavity 211 to the liquid guiding part 100. It should be noted that the driver 300 can be directly connected to the liquid storage chamber 210 as a whole, or can be connected to the liquid storage chamber 210 by a pipeline. When the driver 300 is directly connected to the liquid storage chamber 210 as a whole, in the first state of the flow dividing part, the flow dividing part can first suck the reaction liquid 700 into the driver 300 (when the driver 300 is a cylindrical piston, the reaction liquid 700 can be first sucked into the piston chamber), and in the second state of the flow dividing part, then export the reaction liquid 700 in the piston chamber. When the driver 300 is connected to the liquid storage chamber 210 by a pipeline, in the first state of the flow dividing part, the driver 300 can first suck the reaction liquid 700 into the pipeline between the driver 300 and the liquid storage chamber 210, and in the second state of the flow dividing part, the driver 300 then guides the reaction liquid 700 in the pipeline to the liquid guiding part 100. In other words, the reaction liquid 700 can be stored in the driver 300, or can be stored in the pipeline between the driver 300 and the liquid storage chamber 210, or the reaction liquid 700 can be stored in both the driver 300 and the pipeline between the driver 300 and the liquid storage chamber 210 at the same time.

[0069] To avoid cross-infection of various types of reaction liquids 700 or facilitate the cleaning of the reaction surface 910, in some embodiments, a cleaning liquid (the cleaning liquid belongs to the reaction liquid 700) may be provided in at least one cavity 211 in the liquid storage cavity 210. After the first type of reaction liquid 700 is guided to the reaction surface 910, the cleaning liquid in the liquid storage cavity 210 can be extracted first, and then the cleaning liquid can be discharged. The cleaning liquid can well clean the residual first type of reaction liquid 700 in the pipeline, thereby preventing the subsequent second type of reaction liquid 700 from mixing into the residual first type of reaction liquid 700 on the inner wall of the pipeline and causing pollution of the second type of reaction liquid 700. Moreover, the cleaning liquid can also be used for cleaning the reaction surface 910. After the first type of reaction liquid 700 reacts with the reaction surface 910, guiding the cleaning liquid to the reaction surface 910 can complete the cleaning of the reaction surface 910.

[0070] See Figure 2 , to achieve the purpose of separately discharging the reaction liquid 700 in each cavity 211 in the liquid storage cavity 210, in some other embodiments, the driver 300 may include a plurality of driving monomers 310. Each driving monomer 310 is in one-to-one correspondence and communication with each cavity 211. Each driving monomer 310 can independently generate a driving force or be driven by the same driving component (the driving component belongs to the driver 300) after being respectively connected to a valve. Each driving monomer 310 can separately guide the reaction liquid 700 in the cavity 211 corresponding to it to the liquid guiding part 100. This driving method makes the driving process more accurate.

[0071] See Figure 4 , to achieve the purpose of separately discharging the reaction liquid 700 in each cavity 211 in the liquid storage cavity 210, in some other embodiments, the liquid guiding part 100 includes a plurality of liquid guiding monomers 140. Each liquid guiding monomer 140 is in one-to-one correspondence and communication with each cavity 211. Each liquid guiding monomer 140 is configured to guide the reaction liquid 700 to the reaction surface 910 after obtaining the reaction liquid 700. The driver 300 includes a plurality of driving monomers 310. Each driving monomer 310 is in one-to-one correspondence and communication with each cavity 211, so that each driving monomer 310 separately guides the reaction liquid 700 in the cavity 211 corresponding to it to the liquid guiding monomer 140 corresponding to it. This driving method further improves the accuracy of the driving process and basically avoids cross-infection of different types of reaction liquids 700.

[0072] See Figure 2, in some embodiments, the valve assembly is a rotary valve. Specifically, the liquid storage portion 200 further includes a flow divider, and the flow divider has at least a first state and a second state. In the first state, the flow divider conducts the driver 300 and any one of the cavities 211, so that the driver 300 extracts the reaction liquid 700. In the second state, the flow divider conducts the driver 300 and the liquid guiding portion 100, so that the driver 300 guides the extracted reaction liquid 700 to the liquid guiding portion 100. The flow divider includes a flow dividing disk 220, and the flow dividing disk 220 is provided with a first diversion port 221 and a plurality of second diversion ports 222. Each second diversion port 222 is arranged around the first diversion port 221. The first diversion port 221 communicates with one of the second diversion ports 222 inside the flow dividing disk 220, and the first diversion port 221 communicates with the driver 300. The flow dividing disk 220 is configured to rotate circumferentially around the first diversion port 221, so as to switch between the first state and the second state. In the first state, the second diversion port 222 communicating with the first diversion port 221 communicates with one of the cavities 211. In the second state, the second diversion port 222 communicating with the first diversion port 221 communicates with the liquid guiding portion 100. Specifically, in the first state, the flow dividing disk 220 rotates to make the second diversion port 222 communicating with the first diversion port 221 communicate with the cavity 211 storing the first type of reaction liquid 700. The driver 300 generates a negative pressure, so that the reaction liquid 700 in the cavity 211 flows into the flow dividing disk 220 through the second diversion port 222, flows out of the flow dividing disk 220 through the first diversion port 221 and is stored in the pipeline between the flow dividing disk 220 and the driver 300 (or inside the driver 300). In the second state of the flow dividing disk 220, the flow dividing disk 220 rotates to make the second diversion port 222 communicating with the first diversion port 221 communicate with the liquid guiding portion 100. At this time, the driver 300 can generate a positive pressure, so that the reaction liquid 700 is guided to the liquid guiding portion 100 and is guided to the reaction surface 910 of the sample carrier 900 through the liquid guiding portion 100. In this solution, the state switching of the flow divider and the selection of the reaction liquid 700 are realized by the rotation of the flow dividing plate, and the structure is simple and the cost is low.

[0073] When the reaction on the reaction surface 910 of the sample carrier 900 is completed and another type of reaction liquid 700 needs to be introduced onto the reaction surface 910, the previous reaction liquid 700 needs to be cleaned. In view of this, refer to Figures 5 - 6, in some embodiments, the liquid distribution assembly 10 further includes a cleaning unit 600 and a driving pump 400. The driving pump 400 is connected to the cleaning unit 600 and introduces gas 800 into the cleaning unit 600. The cleaning unit 600 exports the gas 800 to the reaction surface 910 to clean the reaction surface 910. In this solution, the reaction surface 910 is cleaned by an air flow. On the one hand, the cleaning efficiency can be improved, and on the other hand, the damage to the reaction surface 910 can be reduced. Moreover, the air flow exported by the cleaning unit 600 can, on the one hand, clean the reacted reaction liquid 700, and on the other hand, clean the cleaning liquid that wets the reaction surface 910. In some embodiments, when the first type of reaction liquid 700 for reaction is reacted on the reaction surface 910, the cleaning unit 600 can be used to clean the first type of reaction liquid 700 for reaction on the reaction surface 910, and then the cleaning liquid is guided to the reaction surface 910. After the cleaning liquid has completed cleaning, the cleaning unit 600 is used to remove the cleaning liquid, and then the second type of reaction liquid 700 for reaction is arranged on the reaction surface 910. Of course, in other embodiments, the first type of reaction liquid 700 for reaction that has reacted on the reaction surface 910 can be directly removed by the cleaning liquid (that is, the first type of reaction liquid 700 for reaction does not need to be cleaned by the cleaning unit 600), and then the cleaning unit 600 is used to remove the cleaning liquid.

[0074] The cleaning unit 600 and the liquid guiding unit 100 can move independently of each other, and they can also be linked simultaneously. When they move independently of each other, during the process of the liquid guiding unit 100 guiding liquid, the cleaning unit 600 can stay in a standby state and remain stationary. When the cleaning unit 600 is cleaning, the liquid guiding unit 100 can stay in a standby state and remain stationary. However, in the above method, both the cleaning unit 600 and the liquid guiding unit 100 have pauses, resulting in low working efficiency. To improve the working efficiency of the liquid distribution assembly 10, refer to Figures 5 - 6 , in some embodiments, the cleaning unit 600 is connected to the liquid guiding unit 100 and is relatively fixedly arranged with the liquid guiding unit 100. The liquid outlet 116 is arranged adjacent to the gas outlet of the cleaning unit 600. The gas outlet is located on the front side of the liquid outlet 116 along the moving direction of the relative movement with respect to the reaction surface 910, so that when the cleaning unit 600 and the liquid guiding unit 100 move relative to the reaction surface 910, the cleaning unit 600 first cleans the reaction surface 910, and then the liquid guiding unit 100 arranges the reaction liquid 700 on the reaction surface 910. In this solution, the cleaning unit 600 and the liquid guiding unit 100 are connected as a whole, and when the whole formed by the cleaning unit 600 and the liquid guiding unit 100 moves relative to the reaction surface 910 along the second direction X, while the cleaning unit 600 cleans the reaction surface 910, the liquid guiding unit 100 can simultaneously arrange the reaction liquid 700 on the reaction surface 910. In this solution, the cleaning unit 600 and the liquid guiding unit 100 move synchronously, and their working processes are more continuous, improving the processing efficiency.

[0075] One of the processing procedures is as follows: The cleaning unit 600 and the liquid guiding unit 100 move synchronously from the initial position along the second direction X relative to the reaction surface 910. After the cleaning unit 600 cleans the reaction surface 910, the liquid guiding unit 100 synchronously guides the first type of reaction liquid 700 for reaction onto the reaction surface 910. After the reaction is completed (or during the reaction process), the cleaning unit 600 and the liquid guiding unit 100 return to the initial position. After the reaction is completed, the cleaning unit 600 and the liquid guiding unit 100 move synchronously again along the second direction X relative to the reaction surface 910. During this process, the cleaning unit 600 removes the first type of reaction liquid 700 on the reaction surface 910, and the liquid guiding unit 100 synchronously arranges the cleaning liquid onto the reaction surface 910. After the cleaning is completed (or during the cleaning process), the cleaning unit 600 and the liquid guiding unit 100 return to the initial position. After the cleaning is completed, the cleaning unit 600 and the liquid guiding unit 100 move synchronously again along the second direction X relative to the reaction surface 910. During this process, the cleaning unit 600 removes the cleaning liquid, and the liquid guiding unit 100 synchronously arranges the second type of reaction liquid 700 for reaction onto the reaction surface 910. By repeating this process, the reaction process of the reaction surface 910 can be completed.

[0076] To prevent the cleaning unit 600 from blowing the waste liquid onto the reaction liquid 700 to be reacted that is being exported by the liquid guiding unit 100 during the cleaning process, refer to Figures 5 - 8 In some embodiments, the cleaning unit 600 inclines to export the gas 800 in a direction away from the liquid guiding unit 100. Specifically, the cleaning unit 600 may include an air outlet channel 610. The air outlet direction of the air outlet channel 610 intersects with the second direction X. Moreover, one end of the air outlet of the air outlet channel 610 is the first end 611, and the end away from the air outlet is the second end 612. Along the second direction X, the first end 611 is located on the side of the second end 612 away from the liquid guiding unit 100. In this solution, the flow direction of the airflow exported by the cleaning unit 600 is away from the liquid guiding unit 100, so that the residual liquid removed by the cleaning unit 600 moves in a direction away from the liquid guiding unit 100, thereby reducing the risk of the residual liquid splashing onto the reaction liquid 700 to be reacted that is being exported by the liquid guiding unit 100 and improving the purity of the reaction liquid 700 to be reacted.

[0077] The liquid guiding method of the liquid guiding unit 100 depends on actual requirements. Refer to Figures 1 - 5, in some embodiments, the liquid guiding part 100 is configured to export the reaction liquid 700 and then lay it on the reaction surface 910 through viscous force. That is, after the reaction liquid 700 is exported, it naturally falls onto the reaction surface 910 or is adsorbed onto the reaction surface 910 due to the influence of the surface tension of the liquid. This solution has good adaptability for the reaction liquid 700 with relatively high viscosity. In a further embodiment of this solution, the liquid exported by the liquid guiding part 100 can be laid on the reaction surface 910 in a natural flowing manner. For example, the reaction liquid 700 is exported from a small circular liquid guiding port and directed to the reaction surface 910. Due to the action of gravity, the reaction liquid 700 naturally spreads on the reaction surface 910, thus covering the reaction surface 910. In another further embodiment of this solution, the liquid guiding part 100 and the reaction surface 910 can move relative to each other, so that the reaction liquid 700 covers the reaction surface 910. In a further embodiment, the liquid guiding part 100 can be exported from a long strip-shaped liquid outlet 116, and the liquid guiding part 100 moves relative to the reaction surface 910 along the length direction perpendicular to the above liquid outlet 116, so that the reaction liquid 700 covers the reaction surface 910. Of course, in other embodiments, the reaction liquid 700 can also be exported from a circular liquid guiding hole, and the liquid guiding part 100 moves in a scanning manner relative to the reaction surface 910 at various positions, so that the reaction liquid 700 covers the reaction surface 910.

[0078] See Figures 6 - 7 , in some other embodiments, the liquid guiding part 100 includes an atomizing component 118, and the atomizing component 118 is configured to spray the reaction liquid 700 in a mist form onto the reaction surface 910. The atomizing component 118 can specifically be a piezoelectric ceramic microporous atomizer. The atomizing component 118 atomizes the reaction liquid 700, and the atomized reaction surface 910 is sprayed onto the reaction surface 910 and thus adsorbed by the reaction surface 910. In this solution, on the one hand, the accuracy requirement for the distance between the liquid guiding part 100 and the reaction surface 910 is lower, and on the other hand, the laying efficiency of the liquid guiding part 100 is higher and the laying area is larger. This solution has good adaptability for the reaction liquid 700 with relatively low viscosity.

[0079] Figure 6In other embodiments, the liquid distributing assembly 10 further includes a cleaning section 600 and a driving pump 400, wherein the driving pump 400 is connected to the cleaning section 600 and introduces gas 800 into the cleaning section 600, and the cleaning section 600 discharges gas 800 to the reaction surface 910 to clean the reaction surface 910; the liquid guiding section 100 includes an atomizing component 118, and the atomizing component 118 is used to spray the reaction liquid 700 in the form of mist to the reaction surface 910. In particular, the driver 300 and the driving pump 400 are the same component. That is to say, in this solution, one power component can be used to simultaneously drive the gas 800 in the cleaning section 600 and the reaction liquid 700 in the liquid guiding section 100, thereby reducing the number of parts and material costs. Specifically, the driver 300 and the driving pump 400 are the same component and are a compressed air pump. The compressed air pump generates compressed gas 800. A first air valve is connected between the compressed air pump and the liquid guiding part 100, and a second air valve 520 is connected between the compressed air pump and the cleaning part 600. The first air valve 510 adjusts the flow rate of gas 800 guided by the compressed air pump to the liquid guiding part 100, and the second air valve 520 adjusts the flow rate of gas 800 guided by the compressed air pump to the cleaning part 600.

[0080] The specific structure of the liquid guide part 100 depends on actual needs, see Figure 1 as well as Figures 10 - 14 In some embodiments, the liquid guide portion 100 is provided with a liquid inlet channel and a liquid outlet 116, one end of the liquid inlet channel is connected to the liquid storage chamber 210, and the other end is connected to the liquid outlet 116. The liquid outlet 116 is arranged to extend along the first direction Y (that is, the length direction of the liquid outlet 116 is parallel to the first direction Y), and the liquid outlet 116 is configured to move relative to the reaction surface 910 along the second direction X so as to lay the reaction liquid 700 on the reaction surface 910, and the second direction X is perpendicular to the first direction Y and parallel to the reaction surface 910. This solution allows the liquid guide portion 100 to move along a straight line relative to the reaction surface 910 to guide the reaction liquid 700 to the reaction surface 910, and the movement method is simpler and the operation is more convenient.

[0081] Specifically, see Figure 1 as well as Figures 10 - 14In some embodiments, the liquid guide portion 100 includes a first block 110 and a second block 120 connected to the first block 110. A liquid inlet channel is provided in the first block 110, and a first liquid guide groove 114 communicating with the liquid inlet channel is provided on the wall of the first block 110 facing the second block 120, and the first liquid guide groove 114 penetrates the first block 110 along a third direction Z. The third direction Z is perpendicular to the second direction X and the first direction Y, and the second block 120 covers the notch of the first liquid guide groove 114 facing the second block 120, and the first liquid guide groove 114 penetrates the notch of the first block 110 along the third direction Z to form a liquid outlet 116. In this solution, the gap between the first block 110 and the second block 120 is used to define the liquid outlet 116, so that the processing of the liquid outlet 116 is simpler. In this solution, further, the liquid inlet of the liquid inlet channel and the liquid outlet 116 can be located on opposite sides of the liquid guiding portion 100, respectively, so that the flow of the reaction liquid 700 is smoother.

[0082] See also Figure 1 as well as Figures 10 - 14 In some embodiments, the liquid conducting channel includes a first hole 111, a second hole 112, a third hole 113 and a second liquid conducting groove 115. One end of the first hole 111 is connected to the liquid storage chamber 210, and the other end is connected to one end of the second hole 112. The axis of the first hole 111 and the second hole 112 coincide with each other and are both parallel to the third direction Z, and the aperture of the first hole 111 is larger than the aperture of the second hole 112. The first hole 111 can be conveniently inserted with a connecting tube, which is used to conduct the first hole 111 and the liquid outlet cavity. The third hole 113 is connected to the end of the second hole 112 away from the first hole 111, the axis of the third hole 113 is parallel to the second direction X, the end of the third hole 113 away from the second hole 112 is connected to the second liquid conducting groove 115, the length direction of the second liquid conducting groove 115 is parallel to the first direction Y, the second liquid conducting groove 115 passes through the wall of the first block 110 facing the second block 120, and the second liquid conducting groove 115 is connected to the first liquid conducting groove 114. In this solution, the liquid is first directed to the second liquid guiding groove 115, which facilitates the spreading of the reaction liquid 700, so that the liquid guided out of the liquid outlet 116 is more uniform along the first direction Y. In some embodiments, along the second direction X, the groove depth of the second liquid guiding groove 115 is greater than the groove depth of the first liquid guiding groove 114, so that the reaction liquid 700 can be first spread along the first direction Y after being guided into the second liquid guiding groove 115, and then guided out of the first liquid guiding groove 114, further improving the uniformity of the reaction liquid 700 guided out of the liquid outlet 116 along the first direction Y.

[0083] In order to further improve the uniformity of the discharge of the reaction liquid 700, in some embodiments, the third hole 113 may communicate with the middle position of the second liquid guiding groove 115 along the first direction Y. In other embodiments, the liquid guiding channel may include two first holes 111, two second holes 112, and two third holes 113, where one first hole 111, one second hole 112, and one third hole 113 are correspondingly connected, and the other first hole 111, the other second hole 112, and the other third hole 113 are correspondingly connected. One of the third holes 113 and the other third hole 113 are respectively connected to opposite sides of the second liquid guiding groove 115 along the first direction Y, so that the reaction liquid 700 can be introduced into the second liquid guiding groove 115 from opposite sides of the second liquid guiding groove 115 along the first direction Y, making the spreading speed of the reaction liquid 700 in the second liquid guiding groove 115 faster and further improving the uniformity of the discharge of the reaction liquid 700. Of course, in other embodiments, the liquid guiding channel may further include a greater number of first holes 111, second holes 112, and third holes 113, which will not be elaborated here.

[0084] The connection manner between the first block 110 and the second block 120 depends on actual requirements. In this embodiment, the first block 110 and the second block 120 are threadedly connected using threaded fasteners. In other embodiments, the first block 110 and the second block 120 may be riveted, welded, or glued, which will not be elaborated here.

[0085] See Figures 11 - 12 , in some embodiments, the first block 110 includes a first inclined side 117, and the second block 120 includes a second inclined side 121. Along the direction from the liquid outlet 116 to the direction away from the second block 120, the first inclined side 117 is arranged to incline away from the reaction surface 910. Along the direction from the liquid outlet 116 to the direction away from the first block 110, the second inclined side 121 is arranged to incline away from the reaction surface 910. This structure positions the liquid outlet 116 at the tip of the liquid guiding portion 100, effectively preventing the reaction liquid 700 discharged from the liquid outlet 116 from being adsorbed by the end of the liquid guiding body facing the reaction surface 910, thereby causing uneven thickness of the reaction liquid 700.

[0086] The applicant has found that when the viscosity of the reaction liquid 700 is relatively high, the thickness uniformity of the liquid discharged from the liquid guiding portion 100 and laid on the reaction surface 910 is relatively low. To solve the above problem, in some embodiments, the liquid storage portion 200 includes at least one liquid storage cavity 210 for storing a viscous reagent, and the liquid guiding portion 100 further includes a scraping member disposed adjacent to the liquid outlet 116. The scraping member is used to lay the viscous reagent flowing out of the liquid outlet 116 on the reaction surface 910. Specifically, see Figures 8 - 14, in some embodiments, the liquid guiding part 100 includes a liquid scraping protrusion 130 (i.e., the aforementioned scraping member), the liquid scraping protrusion 130 is arranged to extend along the first direction Y, and the liquid scraping protrusion 130 is provided on one side of the liquid outlet 116 along the reverse direction of the second direction X, so that the reaction liquid 700 is first led out from the liquid outlet 116 to the reaction surface 910, and then scraped by the liquid scraping protrusion 130. The liquid scraping protrusion 130 is used to scrape the reaction liquid 700 guided from the liquid outlet 116 to the reaction surface 910 to define the thickness of the reaction liquid 700 after scraping. In this solution, after the reaction liquid 700 is guided from the liquid outlet 116 to the reaction surface 910, a relatively large thickness is formed on the reaction surface 910. When the liquid guiding part 100 and the reaction surface 910 move relative to each other along the second direction X, the reaction liquid 700 led out from the liquid outlet 116 is scraped by the liquid scraping protrusion 130, and the thickness of the reaction liquid 700 after scraping is basically equal to the distance between the liquid scraping protrusion 130 and the reaction surface 910. When the viscosity of the reaction liquid 700 is relatively large, the reaction liquid 700 guided to the reaction surface 910 can also be at the required thickness value, making the reaction result more accurate.

[0087] In some embodiments, the liquid distribution assembly 10 further includes a first driving part (not shown in the figure) connecting the liquid guiding part 100, and the first driving part drives the liquid guiding part 100 to move relative to the reaction surface 910 along the second direction X. In this solution, by driving the liquid guiding part 100 to move, relative movement is generated between the liquid guiding part 100 and the reaction surface 910, the structure is simpler, and the driving is more convenient.

[0088] An embodiment of the second aspect of the present invention further provides a sequencing device, which includes the liquid distribution assembly 10, the carrier table 20 and the reaction liquid 700 in any of the above embodiments. The carrier table 20 is used to carry the sample carrier 900. The reaction liquid 700 is stored in the liquid storage cavity 210 of the liquid distribution assembly 10. Benefiting from the improvement of the liquid distribution assembly 10, the sequencing device simultaneously has all the above advantages of the liquid distribution assembly 10, which will not be elaborated here.

[0089] In some embodiments, the sequencing device further includes a second driving part (not shown in the figure) connecting the carrier table 20, and the second driving part drives the carrier table 20 to move relatively in the reverse direction of the second direction X. In this solution, the carrier table 20 can be driven by the second driving part to move relative to the liquid guiding part 100, so that the liquid guiding part 100 can remain stationary, enabling the liquid guiding part 100 to more accurately lead out the reaction liquid 700. Of course, in other embodiments, the first driving device can also drive the liquid guiding part 100 to move while the second driving part drives the carrier table 20 to move, which will not be elaborated here.

[0090] A third aspect embodiment of the present invention further provides an analysis device, which includes the sequencing device of any one of the above and a sample carrier 900, and the sample carrier 900 is connected to the stage 20. Thanks to the improvement of the liquid distribution component 10, the analysis device simultaneously has all the above advantages of the liquid distribution component 10, which will not be elaborated here.

[0091] It should be noted that if there are directional indications (such as up, down, left, right, front, back...) involved in the embodiments of the present invention, the directional indications are only used to explain the relative positional relationship and movement conditions between components in a certain specific posture. If the specific posture changes, the directional indications will also change accordingly.

[0092] In addition, if there are descriptions such as "first" and "second" involved in the embodiments of the present invention, the descriptions of "first", "second", etc. are only for descriptive purposes and cannot be understood as indicating or implying their relative importance or implicitly indicating the quantity of the indicated technical features. Thus, the features defined with "first" and "second" may explicitly or implicitly include at least one such feature. In addition, if "and / or", "and / or" or "and / or" appear throughout the text, their meanings include three parallel scenarios. Taking "A and / or B" as an example, it includes scenario A, or scenario B, or the scenario where both A and B are satisfied simultaneously. In addition, the technical solutions between the various embodiments can be combined with each other, but it must be based on what can be achieved by those of ordinary skill in the art. When the combination of technical solutions results in contradictions or cannot be achieved, it should be considered that such a combination of technical solutions does not exist and is not within the scope of protection required by the present invention.

[0093] The above are only the preferred embodiments of the present invention, and do not limit the patent scope of the present invention. Any equivalent structural transformation made by using the description of the specification and drawings of the present invention under the inventive concept of the present invention, or directly / indirectly applied in other related technical fields, is included in the patent protection scope of the present invention.

Claims

1. A liquid distribution component for distributing liquid to a sample carrier, characterized in that, The sample carrier has a reaction surface that is open towards the liquid distribution component, and the liquid distribution component includes: A liquid storage part, including a liquid storage cavity for storing a reaction liquid; A liquid guiding part, which is communicated with the liquid storage cavity and is movably arranged opposite to the reaction surface, and is configured to obtain the reaction liquid in the liquid storage cavity and then guide the reaction liquid towards the reaction surface. The liquid guiding part has a coating blade head facing the reaction surface, and the coating blade head includes a liquid outlet. The size of the liquid outlet in a first direction is larger than the size in a second direction that is not parallel to the first direction, and both the first direction and the second direction are parallel to the reaction surface; and, A driver, which is communicated with the liquid storage cavity to drive the reaction liquid in the liquid storage cavity to the liquid guiding part.

2. The liquid distribution component according to claim 1, wherein The first direction is perpendicular to the second direction, the reaction surface includes a reaction site array arranged along the first direction and the second direction, and the size of the liquid outlet along the first direction matches the size of the reaction site array along the first direction.

3. The liquid distribution component according to claim 1, wherein The liquid storage cavity includes a plurality of cavities for separately storing the reaction liquid, and the driver is configured to separately guide the reaction liquid in each cavity to the liquid guiding part.

4. The liquid distribution component according to claim 3, wherein The driver includes a driving pump and a valve assembly connected between the driving pump and the liquid storage cavity; the valve assembly has a first state in which the plurality of cavities are selectively communicated with the driving pump respectively, and a second state in which the driving pump is communicated with the liquid guiding part; Or, The driver includes a plurality of driving units, and each driving unit is correspondingly communicated with each cavity one by one, so that each driving unit separately guides the reaction liquid in the cavity correspondingly communicated with it to the liquid guiding part; Or, The liquid guiding part includes a plurality of liquid guiding units, and each liquid guiding unit is correspondingly communicated with each cavity one by one. Each liquid guiding unit is configured to obtain the reaction liquid and then guide the reaction liquid towards the reaction surface. The driver includes a plurality of driving units, and each driving unit is correspondingly connected between each cavity and each liquid guiding unit.

5. The liquid distribution component according to claim 4, characterized in that, The valve assembly is a rotary valve.

6. The liquid distribution component according to claim 1, wherein The liquid distribution component further includes a cleaning part and a driving pump. The driving pump is communicated with the cleaning part and introduces gas into the cleaning part. The cleaning part has an air outlet for discharging the gas towards the reaction surface to clean the reaction surface.

7. The liquid distribution component according to claim 6, wherein The cleaning part is connected to the liquid guiding part and is relatively fixedly arranged with the liquid guiding part. The liquid outlet is arranged adjacent to the air outlet, and the air outlet is located on the front side of the liquid outlet along the moving direction of the relative movement with respect to the reaction surface.

8. The liquid distribution component according to claim 7, wherein The air outlet is opened on the side of the cleaning part facing away from the liquid guiding part, and the air flow direction of the air outlet is not perpendicular to the reaction surface.

9. The liquid distribution component according to claim 1, wherein the liquid storage part includes at least one liquid storage cavity for storing a viscous reagent, and the liquid guiding part further includes a scraping member disposed adjacent to the liquid outlet, and the scraping member is configured to spread the viscous reagent flowing out of the liquid outlet onto the reaction surface.

10. The liquid distribution component according to claim 1, wherein the liquid guiding part is provided with a liquid inlet channel and a liquid guiding slit, one end of the liquid inlet channel is communicated with the liquid storage cavity, and the other end is communicated with the liquid guiding slit, and the liquid guiding slit forms the liquid outlet on the end surface of the liquid guiding part close to the reaction surface.

11. The liquid distribution component according to claim 10, wherein the liquid guiding part includes a first block and a second block connected to the first block, the liquid inlet channel is arranged in the first block, a first liquid guiding groove communicated with the liquid inlet channel is arranged on the wall surface of the first block facing the second block, the first liquid guiding groove penetrates through the first block along a third direction, the third direction is perpendicular to the first direction and the second direction, the second block covers the notch of the first liquid guiding groove facing the second block, and the notch of the first liquid guiding groove penetrating through the first block along the third direction forms the liquid guiding slit.

12. The liquid distribution component according to claim 9, wherein the scraping member includes a scraping protrusion arranged at the edge of the liquid outlet, and the scraping protrusion is located at the rear side of the liquid outlet along the moving direction of the liquid outlet relative to the reaction surface.

13. A sequencing device, characterized in that, Comprising: the liquid distribution component according to any one of claims 1-12; and, a stage for supporting the sample carrier, and a reaction surface of the sample carrier has a site for supporting a nucleic acid library.

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