Embryo freezing and unfreezing machine

Abstract

The invention provides an embryo freezing and unfreezing machine. The embryo freezing and unfreezing machine comprises a rack, a supporting seat and a driving device, the perforated plate can be supported on the rack, the supporting seat is movably arranged on the rack, the perforated plate is placed at the upper end of the rack, the supporting seat is close to the bottom of the perforated plate from bottom to top, then is in butt joint with the lower end of the perforated plate and heats the bottom of a hole grid, containing unfreezing liquid, of the perforated plate, and the driving device is used for driving the supporting seat to move. In the embodiment of the utility model, one hole grid of the perforated plate is heated through the supporting seat, so that the thawing liquid, the diluent, the No.1 flushing liquid and the No.2 flushing liquid are simultaneously positioned on the same perforated plate, and an embryo is transferred on the same perforated plate during embryo thawing and is prevented from falling out of the perforated plate.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to an embryo freezing and thawing machine. Background Art

[0002] In vitro fertilization technology is constantly developing and innovating. When doing in vitro fertilization, considering that the patient may have an illness or the patient does not have sufficient time, you can choose to freeze the embryos first and then thaw and transplant them when the time is right.

[0003] When thawing embryos, the following operations need to be performed:

[0004] 1. Take out the straw from the liquid nitrogen tank and place the embryo from the straw into the 37°C thawing solution for 1 minute;

[0005] 3. Use a Pasteur tube to transfer the embryos from the thawing solution to the room temperature diluent for 3 minutes, rinse solution No. 1 for 5 minutes, and rinse solution No. 2 for 5 minutes.

[0006] In the above thawing process, the 37°C thawing solution is loaded in a separate container, and the remaining diluent, No. 1 flushing solution and No. 2 flushing solution are loaded separately in a multi-well plate. When the medical staff operates, when transferring the embryos from the thawing solution to the multi-well plate, there is a certain probability that the embryos will fall off during the transfer process, and there is also a certain probability that the order will be confused when placing the embryos in the solution on the multi-well plate, thereby placing the embryos in the wrong solution. Summary of the invention

[0007] In view of the deficiencies in the prior art, the present invention provides an embryo freezing and thawing machine, which can solve or at least alleviate one or more of the above-mentioned problems and other problems in the prior art.

[0008] The present invention provides an embryo freezing and thawing machine, comprising:

[0009] A frame supports the porous plate; a support seat is movably arranged on the frame, can approach and support the porous plate from below, and can heat one of the holes on the porous plate; and a driving device is used to drive the support seat to move.

[0010] Preferably, the support base is hollow and filled with water; the embryo freezing and thawing machine further includes: a heating ring connected to the upper end surface of the support base, internally communicating with the inside of the support base, and the heating ring can be sleeved on the outer periphery of the bottom of one of the cell grids of the porous plate after moving up with the support base; one or more limiting rings connected to the upper end surface of the support base, and the limiting rings can be sleeved on the outer periphery of the bottom of one of the cell grids of the porous plate after moving up with the support base; and a heating device for heating the water inside the support base; the water inside the support base overflows into the heating ring and can perform water bath heating on the bottom of the cell grid of the porous plate inserted into the heating ring.

[0011] Preferably, a liquid injection device is provided on the frame; the frame includes: a support plate horizontally arranged, with a strip-shaped relief opening extending in the horizontal direction formed thereon; and a square sleeve connected to the support plate, with a plurality of porous plates stacked therein, and the bottom of the lower porous plate abuts against the support plate; the upper end of the support base can pass through the strip-shaped relief opening to lift the porous plate upward; the support base can limit the horizontal direction of the porous plate; the support base can move along the length direction of the strip-shaped relief opening; the liquid injection device is located above the strip-shaped relief opening; the liquid injection device can respectively inject thawing liquid, diluting liquid, No. 1 rinsing liquid, and No. 2 rinsing liquid into different cell grids of the porous plate on the support base.

[0012] Preferably, the driving device includes: a sliding seat horizontally slidably arranged on the frame and located below the support plate; a guiding seat connected to the upper end of the sliding seat, with a square guiding hole formed thereon, and the guiding seat is located below the support plate; a guiding rod, square, in vertical sliding fit with the guiding hole, and the upper end of the guiding rod passes through the guiding hole and is connected to the lower end of the support base; and a driving mechanism for driving the guiding rod to move vertically and also for driving the sliding seat to slide in the length direction of the strip-shaped relief opening.

[0013] Preferably, the driving mechanism includes: a limiting block connected to the sliding seat, with a threaded hole formed thereon; a linkage rod, the upper end of which passes through the threaded hole and is rotatably connected to the guiding rod, and the linkage rod is threadedly connected to the threaded hole on the limiting block; a motor fixedly connected to the sliding seat; a rotating shaft coaxially connected to the output end of the motor; and a linkage assembly for converting the rotation of the rotating shaft into the rotation of the linkage rod.

[0014] Preferably, the linkage assembly includes: a driving block connected to the outer wall of the lower end of the linkage rod; a driving sleeve sleeved outside the linkage rod, the lower end of the driving sleeve being coaxially connected to the upper end of the rotating shaft, and the driving sleeve being provided with a first chute and a second chute extending vertically and penetrating therethrough; a first slider slidably arranged vertically in the first chute; a first guiding block located between the driving sleeve and the linkage rod, the outer wall of which is connected to the first slider, and the downward end of the first guiding block having an inclined first inclined surface and a vertical first vertical surface; a second slider slidably arranged vertically in the second chute; and a second guiding block located between the driving sleeve and the linkage rod, the outer wall of which is connected to the second slider, and the upward end of the second guiding block having an inclined second inclined surface and a vertical second vertical surface; the driving block can abut against the first inclined surface, the first vertical surface, the second inclined surface and the second vertical surface; the inclination directions of the first inclined surface and the second inclined surface relative to the sliding seat are the same; and the first slider and the second slider tend to approach each other.

[0015] Preferably, the linkage assembly further includes: a first sliding ring slidably sleeved outside the driving sleeve and connected to the end of the first slider away from the first guiding block; a second sliding ring slidably sleeved outside the driving sleeve and connected to the end of the second slider away from the second guiding block; and a spring sleeved outside the driving sleeve, with both ends respectively abutting against the first sliding ring and the second sliding block.

[0016] Preferably, the driving mechanism includes: a first wire reel coaxially connected to the rotating shaft, and a wire groove is provided on its outer periphery; a second wire reel coaxially connected to the rotating shaft, and a wire groove is provided on its outer periphery; a first pulley rotatably arranged on the frame; a second pulley rotatably arranged on the frame, and the second pulley and the first pulley are located on both sides of the rotating shaft; and a driving rope, one end of which is wound and connected to the first wire reel, and the other end is wound around the first pulley and the second pulley in sequence and then wound and connected to the second wire reel; the driving ropes outside the first wire reel and the second wire reel are in a ring shape; when the rotating shaft rotates, one of the first wire reel and the second wire reel winds the driving rope and the other releases the driving rope; and the driving ropes outside the first wire reel and the second wire reel can remain stationary.

[0017] Preferably, the driving mechanism further includes: a first stop block arranged on one side of the first pulley, and a first limiting hole for the driving rope to pass through is provided thereon; a second stop block arranged on one side of the second pulley, opposite to the first stop block, and a first limiting hole for the driving rope to pass through is provided thereon; and a stop block connected to the driving rope, located between the first stop block and the second stop block, and can respectively abut against the first stop block and the second stop block.

[0018] Preferably, two first magnetic attraction blocks are connected to the frame; the two first magnetic attraction blocks are located on both sides of the sliding seat; second magnetic attraction blocks are respectively connected to both ends of the sliding seat; the second magnetic attraction blocks can be magnetically attracted to the first magnetic attraction blocks on the same side.

[0019] Compared with the prior art, the present invention has the following beneficial effects:

[0020] In the technology of the present invention, a pore cell of the porous plate is heated through the support seat, so that the thawing solution, the dilution solution, the No. 1 rinsing solution, and the No. 2 rinsing solution are simultaneously located on one porous plate. When thawing embryos, the embryos are transferred on one porous plate, avoiding the embryos from falling outside the porous plate. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] In order to more clearly illustrate the specific embodiments of the present invention or the technical solutions in the prior art, the following will briefly introduce the drawings required for the description of the specific embodiments or the prior art. In all the drawings, similar elements or parts are generally identified by similar reference numerals. In the drawings, the elements or parts are not necessarily drawn to scale.

[0022] Figure 1 Schematic diagram of the cooperation of an embryo freezing and thawing machine in an embodiment of the present invention with a desktop;

[0023] Figure 2 For Figure 1 the three-dimensional view of the embryo freezing and thawing machine in

[0024] Figure 3 For Figure 2 the internal structure schematic diagram of

[0025] Figure 4 For Figure 3 the three-dimensional view of the support seat and the porous plate in

[0026] Figure 5 For Figure 3 another three-dimensional view of

[0027] Figure 6 For Figure 5 the sectional view of the SS plane of

[0028] Figure 7 For Figure 6 the enlarged view at A of

[0029] Figure 8 For Figure 5 the three-dimensional view at the driving mechanism in

[0030] Figure 9 For Figure 5Stereogram of the middle linkage component (the first sliding ring, the second sliding ring, the spring and the driving sleeve are successively missing from left to right);

[0031] Figure 10 For Figure 5 Another stereogram of the driving mechanism in the middle.

[0032] Reference numerals:

[0033] 10. Perforated plate; 11. Outer frame; 12. Cell;

[0034] 20. Frame; 21. Liquid injection device; 22. Support plate; 23. Strip-shaped relief opening; 24. Square sleeve;

[0035] 30. Support base; 31. Heating ring; 32. Limiting ring;

[0036] 40. Driving device; 41. Sliding seat; 42. Guide seat; 43. Guide hole; 44. Guide rod;

[0037] 50. Driving mechanism; 51. Limiting block; 52. Linking rod; 53. Motor; 54. Rotating shaft; 55. First wire reel; 56. Second wire reel; 57. First pulley; 58. Second pulley; 59. Driving rope;

[0038] 60. Linkage component; 61. Driving block; 62. Driving sleeve; 621. First chute; 622. Second chute; 63. First slider; 64. First guide block; 641. First inclined surface; 642. First vertical surface; 65. Second slider; 66. Second guide block; 661. Second inclined surface; 662. Second vertical surface; 67. First sliding ring; 68. Second sliding ring; 69. Spring;

[0039] 70. First stop block; 71. Second stop block; 73. Stopping block;

[0040] 80. First magnetic attraction block; 81. Second magnetic attraction block. Detailed implementation manners

[0041] Hereinafter, embodiments of the technical solutions of the present invention will be described in detail with reference to the drawings. The following embodiments are only used to illustrate the technical solutions of the present invention more clearly, and thus are only examples and cannot be used to limit the protection scope of the present invention.

[0042] It should be noted that, unless otherwise specified, the technical terms or scientific terms used in this application should have the ordinary meanings understood by those skilled in the art to which the present invention belongs.

[0043] In the description of the present application, it should be understood that the orientation or positional relationship indicated by terms such as "center", "longitudinal", "transverse", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. is based on the orientation or positional relationship shown in the drawings, and is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a limitation to the present invention.

[0044] In addition, terms such as "first", "second", etc. are only used for descriptive purposes and cannot be understood as indicating or implying relative importance or implicitly specifying the quantity of the indicated technical features. In the description of the present invention, the meaning of "a plurality of" is two or more unless otherwise specifically defined.

[0045] In the present application, unless otherwise clearly specified and limited, terms such as "installed", "connected", "connected to", "fixed", etc. should be understood in a broad sense. For example, it can be a fixed connection, a detachable connection, or integrated; it can be a mechanical connection or an electrical connection; it can be directly connected or indirectly connected through an intermediate medium, and can be the communication inside two elements or the interaction relationship between two elements. For those of ordinary skill in the art, the specific meanings of the above terms in the present invention can be understood according to specific circumstances.

[0046] In the present application, unless otherwise clearly specified and limited, the first feature being "on" or "under" the second feature can be that the first and second features are in direct contact, or the first and second features are indirectly in contact through an intermediate medium. Moreover, the first feature being "above", "over" and "on" the second feature can be that the first feature is directly above or obliquely above the second feature, or merely indicates that the first feature has a higher horizontal height than the second feature. The first feature being "under", "beneath" and "under" the second feature can be that the first feature is directly below or obliquely below the second feature, or merely indicates that the first feature has a lower horizontal height than the second feature.

[0047] See Figures 1 to 10 , the porous plate 10 includes an outer frame 11 and a plurality of bottle-shaped cell grids 12 arranged therein; the cell grids 12 are connected to the outer frame 11. The cell grids 12 are used to load different solutions. When thawing, a four-well plate can be selected for the porous plate 10, and a thawing solution, a dilution solution, a No. 1 rinsing solution, and a No. 2 rinsing solution are respectively injected into different cell grids 12.

[0048] This embodiment provides an embryo freezing and thawing machine, which includes a frame 20, a support seat 30, and a driving device 40.

[0049] The porous plate 10 can be supported on the upper surface of the frame 20. The frame 20 is usually installed on the desktop. An installation window is opened on the desktop, and the frame 20 is placed vertically into the installation window from above. The support base 30 is movably arranged on the frame 20 and can approach and support the porous plate 10 from below, and can heat one of the cell grids 12 on the porous plate 10. The porous plate 10 is placed at the upper end of the frame 20. The support base 30 approaches the bottom of the porous plate 10 from bottom to top, then docks with the lower end of the porous plate 10, and heats the bottom of one of the cell grids 12 of the porous plate 10 containing the thawing solution. The driving device 40 is used to drive the movement of the support base 30.

[0050] In this embodiment, one of the cell grids 12 of the porous plate 10 is heated by the support base 30, so that the thawing solution, the dilution solution, the No. 1 rinsing solution and the No. 2 rinsing solution are all located on one porous plate 10 at the same time. When thawing embryos, the embryos are transferred on one porous plate 10, avoiding the embryos falling outside the porous plate 10.

[0051] In one embodiment, the support base 30 is hollow and filled with water.

[0052] The embryo freezing and thawing machine further includes a heating ring 31, a limiting ring 32 and a heating device (not shown).

[0053] The heating ring 31 is connected to the upper end surface of the support base 30, and its interior is communicated with the interior of the support base 30. The heating ring 31 can be sleeved on the outer periphery of the bottom of one of the cell grids 12 of the porous plate 10 after moving up with the support base 30. One or more limiting rings 32 are provided. The limiting rings 32 are connected to the upper end surface of the support base 30. The limiting rings 32 can be sleeved on the outer periphery of the bottom of one of the cell grids 12 of the porous plate 10 after moving up with the support base 30. When the porous plate 10 is a four-cell plate, three limiting rings 32 can be provided to cooperate with the heating ring 31 to limit the bottoms of the four cell grids 12 on the four-cell plate. The heating device is used to heat the water inside the support base 30. The heating device is a prior art and will not be elaborated here. The water in the support base 30 overflows into the heating ring 31 and can perform water bath heating on the bottom of the cell grid 12 of the porous plate 10 inserted into the heating ring 31.

[0054] In this embodiment, the water inside the support base 30 is heated by the heating device. The heated water in the support base 30 overflows into the heating ring 31. After the support base 30 moves up and the heating ring 31 docks with the bottom of one of the cell grids 12 of the porous plate 10, the heated water performs water bath heating on the bottom of the cell grid 12, thereby performing water bath heating on the thawing solution in the cell grid 12, so that the thawing solution is maintained at 37 degrees.

[0055] In one embodiment, a liquid injection device 21 is provided on the frame 20. The frame 20 includes a support plate 22 and a square sleeve 24.

[0056] The support plate 22 is horizontally arranged, and a strip-shaped relief opening 23 extending in the horizontal direction is formed in the support plate 22.

[0057] The square sleeve 24 is connected to the support plate 22, and a plurality of porous plates 10 are stacked and placed therein, and the bottom of the lower porous plate 10 abuts against the support plate 22. A connecting member is fixedly connected to the outer wall of the square sleeve 24, and the connecting member is fixedly connected to the support plate 22. The upper end of the support seat 30 can pass through the strip-shaped relief opening 23 and then lift the porous plate 10 upward. The support seat 30 can limit the horizontal direction of the porous plate 10. Specifically, the support seat 30 limits the porous plate 10 through the heating ring 31 and the limiting ring 32 thereon, so as to prevent the porous plate 10 from moving horizontally on the support seat 30. The support seat 30 can move along the length direction of the strip-shaped relief opening 23. The liquid injection device 21 is located above the strip-shaped relief opening 23, and the liquid injection device 21 can inject thawing liquid, diluting liquid, No. 1 flushing liquid and No. 2 flushing liquid into different cell grids 12 of the porous plate 10 on the support seat 30 respectively. Specifically, the liquid injection device 21 can be arranged with reference to the prior art. For example, four syringes respectively loaded with thawing liquid, diluting liquid, No. 1 flushing liquid and No. 2 flushing liquid can be arranged, and the electric or starting drive is used to drive the syringe to push the solution.

[0058] In this embodiment, under the drive of the drive device 40, the support seat 30 first moves upward to support and limit the porous plate 10 in the square sleeve 24, and then the support seat 30 horizontally moves in the strip-shaped relief opening 23, so as to drive the porous plate 10 to horizontally move and pass through the liquid injection device 21. The liquid injection device 21 injects thawing liquid, diluting liquid, No. 1 flushing liquid and No. 2 flushing liquid into the porous plate 10. Since the positions of various solutions on the porous plate 10 are fixed each time, it is not easy to be confused. During the movement of the porous plate 10, the support seat 30 performs water bath heating on the grid loaded with thawing liquid on the porous plate 10. Finally, the support seat 30 drives the porous plate 10 to move to one end of the strip-shaped relief opening 23. At this time, the embryo can be placed in the thawing liquid on the porous plate 10.

[0059] In one embodiment, the drive device 40 includes a sliding seat 41, a guide seat 42, a guide rod 44 and a drive mechanism 50.

[0060] The sliding seat 41 is horizontally slidably arranged on the frame 20, and the sliding seat 41 is located below the support plate 22. The guide seat 42 is connected to the upper end of the sliding seat 41. A square guide hole 43 is formed in the guide seat 42, and the guide seat 42 is located below the support plate 22. The cross section of the guide rod 44 is square, and the guide rod 44 is in vertical sliding fit with the guide hole 43. The upper end of the guide rod 44 passes through the guide hole 43 and is connected to the lower end of the support seat 30. The drive mechanism 50 is used to drive the guide rod 44 to move vertically, and the drive mechanism 50 is also used to drive the sliding seat 41 to slide in the length direction of the strip-shaped relief opening 23, so as to drive the support seat 30 to move vertically and horizontally.

[0061] In this embodiment, when thawing the embryo, the embryo stays in the thawing solution for 1 minute, in the dilution solution for 3 minutes, in the No. 1 cleaning solution for 5 minutes, and in the No. 2 cleaning solution for 5 minutes. After the grid 12 where the thawing solution is located is heated to an appropriate temperature by the support base 30, the embryo is placed for thawing. Then the embryo is sequentially transferred to the dilution solution, the No. 1 cleaning solution and the No. 2 cleaning solution. The embryo spends a total of 13 minutes in the subsequent several solutions, and then the entire thawing process is completed. Only then does the support base 30 move downward and then horizontally move under the square sleeve 24 and then move upward to dock with a new multi-well plate 10, and drive the multi-well plate 10 to horizontally move to under the liquid injection device 21 to inject various solutions. Then the support base 30 can perform water bath heating on the thawing solution in the multi-well plate 10. The advantage of water bath heating is that the heating is relatively gentle and the subsequent heat preservation is also stable. However, the water bath heating efficiency is low and it takes a lot of time, which causes a large delay in time from each embryo thawing to the next embryo thawing, and the efficiency is low.

[0062] In this embodiment, driven by the driving mechanism 50, the support base 30 can move vertically up and down and horizontally. After the support base 30 moves upward to dock with a multi-well plate 10 and horizontally moves to under the liquid injection device 21 for liquid injection, the support base 30 raises the temperature and keeps warm the thawing solution in the multi-well plate 10, and then moves to the end of the strip-shaped relief opening 23. After the embryo is transferred from the thawing solution to the dilution solution, the support base 30 moves downward, the support base 30 is separated from the current multi-well plate 10, and then the support base 30 horizontally moves to under the square sleeve 24 to re-dock with a multi-well plate 10 and transport it to under the liquid injection device 21 for liquid injection, and then raises the temperature and keeps warm the thawing solution in the multi-well plate 10. At this time, the embryo in the previous multi-well plate 10 is still in the dilution solution. During the time of passing through the subsequent solutions, the thawing solution in the latter multi-well plate 10 has been heated and kept warm, thus reducing the time in the whole process and further improving the efficiency. In addition, the driving mechanism 50 drives the support base 30 to move to replace the multi-well plate 10, with higher efficiency and stronger stability.

[0063] In one embodiment, the driving mechanism 50 includes a limit block 51, a linkage rod 52, a motor 53, a rotating shaft 54 and a linkage assembly 60.

[0064] The limit block 51 is connected to the sliding seat 41, and a threaded hole is opened thereon. The upper end of the linkage rod 52 passes through the threaded hole and is rotatably connected to the guide rod 44. Specifically, a hole is opened at the lower end of the guide rod 44, and an inner wall of the hole is connected with a bearing. The upper end of the linkage rod 52 is inserted into the hole and connected to the inner ring of the bearing. The linkage rod 52 is threadedly connected to the threaded hole on the limit block 51. The motor 53 is fixedly connected to the sliding seat 41. The rotating shaft 54 is coaxially connected to the output end of the motor 53. The linkage assembly 60 is used to convert the rotation of the rotating shaft 54 into the rotation of the linkage rod 52.

[0065] In this embodiment, driven by the motor 53, the rotating shaft 54 rotates. Through the linkage of the linkage assembly 60, the linkage rod 52 is driven to rotate, and thus the vertical movement of the linkage rod 52 is realized.

[0066] In one embodiment, the linkage assembly 60 includes a driving block 61, a driving sleeve 62, a first slider 63, a first guiding block 64, a second slider 65, and a second guiding block 66.

[0067] The driving block 61 is connected to the outer wall of the lower end of the linkage rod 52. The driving sleeve 62 is sleeved outside the linkage rod 52. The lower end of the driving sleeve 62 is coaxially connected to the upper end of the rotating shaft 54. The driving sleeve 62 is provided with a first chute 621 and a second chute 622 that extend vertically and penetrate. The first slider 63 is slidably arranged vertically in the first chute 621.

[0068] The first guiding block 64 is located between the driving sleeve 62 and the linkage rod 52. Its outer wall is connected to the first slider 63. The lower end of the first guiding block 64 has an inclined first inclined surface 641 and a vertical first vertical surface 642. The second slider 65 is slidably arranged vertically in the second chute 622. The second guiding block 66 is located between the driving sleeve 62 and the linkage rod 52. Its outer wall is connected to the second slider 65. The upper end of the second guiding block 66 has an inclined second inclined surface 661 and a vertical second vertical surface 662.

[0069] The first guiding block 64 and the second guiding block 66 are generally triangular as a whole. Viewed from the axial direction of the rotating shaft 54, the first guiding block 64 and the second guiding block 66 are arc-shaped blocks.

[0070] The driving block 61 can abut against the first inclined surface 641, the first vertical surface 642, the second inclined surface 661, and the second vertical surface 662; the inclined directions of the first inclined surface 641 and the second inclined surface 661 relative to the sliding seat 41 are the same; the first slider 63 and the second slider 65 tend to approach each other.

[0071] In this embodiment, as Figure 9 , as the driving sleeve 62 rotates counterclockwise, the second vertical surface of the lower second guiding block 66 contacts the driving block 61. At this time, the second guiding block 66 drives the driving block 61 to rotate counterclockwise together. At this time, the driving block 61 drives the linkage rod 52 to rotate counterclockwise, and the linkage rod 52 moves upward. Thus, the support seat 30 is used to support the upper porous plate 10. Until the driving block 61 moves upward and disengages from the second vertical surface 662 of the second guiding block 66, at this time, the driving block 61 abuts against the first inclined surface 641 of the first guiding block 64. At this time, when the driving sleeve 62 continues to rotate counterclockwise, since the first guiding block 64 abuts against the driving block 61 with the first inclined surface 641, the first guiding block 64 moves upward along the driving block 61 without pushing the driving block 61 to rotate, so that the linkage rod 52 does not move up and down.

[0072] When the support base 30 needs to be lowered, the drive sleeve 62 rotates clockwise driven by the motor 53. The first vertical surface 642 of the first guide block 64 abuts against the drive block 61 after rotating one circle, thereby driving the drive block 61 to rotate clockwise, and then the linkage rod 52 is lowered until the drive block 61 disengages from the first guide block 64. At this time, the second inclined surface 661 of the second guide block 66 abuts against the drive block 61. Even if the drive sleeve 62 continues to rotate clockwise, the drive block 61 abuts against the inclined surface of the second guide block 66, and the second guide block 66 moves downward without driving the drive block 61 to rotate.

[0073] In one embodiment, the linkage assembly 60 further includes a first sliding ring 67, a second sliding ring 68 and a spring 69.

[0074] The first sliding ring 67 is slidably sleeved outside the drive sleeve 62, and the first sliding ring 67 is connected to one end of the first slider 63 away from the first guide block 64. The second sliding ring 68 is slidably sleeved outside the drive sleeve 62, and the second sliding ring 68 is connected to one end of the second slider 65 away from the second guide block 66. The spring 69 is sleeved outside the drive sleeve 62, and both ends of the spring 69 abut against the first sliding ring 67 and the second sliding block respectively.

[0075] In this embodiment, under the action of the spring 69, the first guide block 64 and the second guide block 66 tend to approach each other, so that the position where the drive block 61 separates from the first guide block 64 is determined, and the position where the drive block 61 separates from the second guide block 66, thereby controlling the height of the support base 30 after moving up or down.

[0076] In one embodiment, the drive mechanism 50 includes a first wire reel 55, a second wire reel 56, a first pulley 57, a second pulley 58 and a drive rope 59.

[0077] The first wire reel 55 is coaxially connected to the rotating shaft 54, and a wire groove is provided on its outer periphery. The second wire reel 56 is coaxially connected to the rotating shaft 54, and a wire groove is provided on its outer periphery. The first pulley 57 is rotatably arranged on the frame 20. The second pulley 58 is rotatably arranged on the frame 20, and the second pulley 58 and the first pulley 57 are located on both sides of the rotating shaft 54. One end of the drive rope 59 is wound and connected to the first wire reel 55, and the other end of the drive rope 59 is wound and connected to the second wire reel 56 after passing around the first pulley 57 and the second pulley 58 in sequence.

[0078] The drive rope 59 outside the first wire reel 55 and the second wire reel 56 is in a ring shape; when the rotating shaft 54 rotates, among the first wire reel 55 and the second wire reel 56, one winds up the drive rope 59 and the other releases the drive rope 59; the drive rope 59 outside the first wire reel 55 and the second wire reel 56 can remain stationary.

[0079] In this embodiment, as Figure 10, the shaft 54 ​​rotates counterclockwise, the first wire drum 55 rotates counterclockwise to reel the driving rope 59, at this time the sliding seat 41 will encounter a certain resistance, so that the sliding seat 41 remains in place, and the second wire drum 56 releases the driving rope 59. At the same time, the shaft 54 ​​rotates counterclockwise to drive the linkage rod 52 to move upward to achieve the support seat 30 to move upward and dock with the porous plate 10. Then the driving rope 59 remains stationary, the shaft 54 ​​continues to rotate counterclockwise, the support seat 30 remains at a certain height, and the first wire drum 55 reels the driving rope 59 to drive the sliding seat 41 to move leftward, and then drive the upper support seat 30 to move leftward and reach the end of the strip-shaped gap after passing through the injection device 21, and the embryo is placed.

[0080] After the embryos are thawed from the thawing solution and transferred to the diluent of the porous plate 10, the support seat 30 is no longer needed to keep the thawing solution of the porous plate 10 warm. The rotating shaft 54 ​​rotates forward, and the driving rope 59 can be moved at this time, so that the sliding seat 41 remains in place, the first wire drum 55 releases the driving rope 59, and the second wire drum 56 reels the driving rope 59. The rotating shaft 54 ​​rotates clockwise to drive the supporting seat 30 to move downward, and the porous plate 10 is supported by the supporting plate 22 above. Then the driving rope 59 remains stationary, and the rotating shaft 54 ​​continues to rotate clockwise, thereby driving the sliding seat 41 to move to the right, and the supporting seat 30 returns to the bottom of the square sleeve 24, completing a pick-up and delivery action of the porous plate 10. This allows a single motor 53 to drive the entire device, which is more concise, and the failure rate of the single motor 53 is also low. In addition, the single motor 53 can save cost and space.

[0081] The rotation of the rotating shaft 54 ​​can drive the sliding seat 41 to move horizontally, and can also drive the supporting seat 30 to move up and down. In addition, during the horizontal movement of the sliding seat 41, the supporting seat 30 is kept at a certain height and does not move up and down.

[0082] In one embodiment, the driving mechanism 50 further includes a first stopper 70 , a second stopper 71 and a stopper 73 .

[0083] The first stopper 70 is arranged at one side of the first pulley 57, and is provided with a first limiting hole for the driving rope 59 to pass through. The second stopper 71 is arranged at one side of the second pulley 58, opposite to the first stopper 70, and is provided with a first limiting hole for the driving rope 59 to pass through. The stopper 73 is connected to the driving rope 59, and is located between the first stopper 70 and the second stopper 71, and the stopper 73 can abut against the first stopper 70 and the second stopper 71 respectively.

[0084] In this embodiment, the rotation of the rotating shaft 54 drives the driving rope 59 to rotate and also drives the linkage rod 52 to move up and down. When the rotating shaft 54 drives the linkage rod 52 to move up and down, the stop block 73 moves between the first stop block 70 and the second stop block 71. Once the stop block 73 abuts against the first stop block 70 or the second stop block 71, it means that the driving rope 59 can no longer move further. At this time, the rotation of the rotating shaft 54 drives the sliding seat 41 to slide on the frame 20.

[0085] In one embodiment, two first magnetic attraction blocks 80 are connected to the frame 20. The two first magnetic attraction blocks 80 are located on both sides of the sliding seat 41. The two ends of the sliding seat 41 are respectively connected with second magnetic attraction blocks 81; the second magnetic attraction blocks 81 can be magnetically attracted to the first magnetic attraction blocks 80 on the same side. The two first magnetic attraction blocks 80 are respectively located near the first pulley 57 and the second pulley 58. When the sliding seat 41 slides to near the first pulley 57 or the second pulley 58, the corresponding second magnetic attraction blocks 81 are magnetically attracted to the first magnetic attraction blocks 80 together, so that the sliding seat 41 is not prone to sliding. Furthermore, when the rotating shaft 54 rotates, when the stop block 73 on the driving rope 59 does not abut against the first stop block 70 or the second stop block 71, the rotation of the rotating shaft 54 only drives the support seat 30 to move up and down.

[0086] In the description of the present invention, a large number of specific details are set forth. However, it can be understood that the embodiments of the present invention may be practiced without these specific details. In some instances, well-known methods, structures, and technologies are not shown in detail so as not to obscure the understanding of this description.

[0087] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some or all of the technical features; and these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the scope of the technical solutions of the embodiments of the present invention, and they should all be covered by the scope of the claims and the description of the present invention.

Claims

1. An embryo freezing and thawing machine, characterized in that: include: A frame (20) on which the porous plate (10) is supported; A support seat (30) is movably arranged on the frame (20), and can approach and support the porous plate (10) from below, and can heat one of the wells (12) on the porous plate (10); A driving device (40) is used for driving the supporting seat (30) to move.

2. An embryo freezing and thawing machine as claimed in claim 1, characterized in that: The support seat (30) is hollow and filled with water; The embryo freezing and thawing machine also includes: A heating ring (31) is connected to the upper end surface of the support seat (30) and is communicated with the interior of the support seat (30). The heating ring (31) can be moved upward along with the support seat (30)H and then sleeved on the outer periphery of the bottom of one of the cells (12) of the porous plate (10); One or more limiting rings (32) are provided and connected to the upper end surface of the support seat (30). The limiting ring (32) can be sleeved on the bottom periphery of one of the cells (12) of the porous plate (10) after the support seat (30) moves upward; and A heating device, used for heating water inside the support seat (30); The water in the support seat (30) overflows into the heating ring (31) and can heat the bottom of the wells (12) of the porous plate (10) inserted into the heating ring (31) in a water bath.

3. An embryo freezing and thawing machine as claimed in claim 1, characterized in that: The frame (20) is provided with a liquid injection device (21); The frame (20) comprises: A support plate (22) is arranged horizontally and is provided with a strip-shaped clearance opening (23) extending in a horizontal direction; and A square sleeve (24) is connected to the support plate (22), wherein a plurality of porous plates (10) are stacked and placed, and the bottom of the lower porous plate (10) abuts against the support plate (22); The upper end of the support seat (30) can pass through the strip-shaped clearance opening (23) to lift the porous plate (10) upwards; the support seat (30) can limit the horizontal direction of the porous plate (10); the support seat (30) can move along the length direction of the strip-shaped clearance opening (23); the injection device (21) is located above the strip-shaped clearance opening (23); the injection device (21) can respectively inject thawing liquid, diluent, No. 1 flushing liquid and No. 2 flushing liquid into different wells (12) of the porous plate (10) on the support seat (30).

4. An embryo freezing and thawing machine as claimed in claim 3, characterized in that: The driving device (40) comprises: A sliding seat (41) is horizontally slidably disposed on the frame (20) and is located below the support plate (22); A guide seat (42) connected to the upper end of the sliding seat (41) and having a square guide hole (43) thereon, wherein the guide seat (42) is located below the support plate (22); A guide rod (44) is square and vertically slidably matched with the guide hole (43); the upper end of the guide rod (44) passes through the guide hole (43) and is connected to the lower end of the support seat (30); and The driving mechanism (50) is used to drive the guide rod (44) to move vertically, and is also used to drive the sliding seat (41) to slide in the length direction of the strip-shaped clearance opening (23).

5. An embryo freezing and thawing machine as claimed in claim 4, characterized in that: The driving mechanism (50) comprises: A limit block (51) connected to the sliding seat (41) and having a threaded hole; A linkage rod (52), the upper end of which passes through the threaded hole and is rotatably connected to the guide rod (44), and the linkage rod (52) is threadedly connected to the threaded hole on the limit block (51); A motor (53) fixedly connected to the sliding seat (41); a rotating shaft (54) coaxially connected to an output end of the motor (53); and The linkage assembly (60) is used to convert the rotation of the rotating shaft (54) into the rotation of the linkage rod (52).

6. An embryo freezing and thawing machine as claimed in claim 5, characterized in that: The linkage assembly (60) comprises: A driving block (61) connected to the outer wall of the lower end of the linkage rod (52); A driving sleeve (62) is sleeved outside the linkage rod (52), the lower end of the driving sleeve (62) is coaxially connected to the upper end of the rotating shaft (54), and the driving sleeve (62) is provided with a first sliding groove (621) and a second sliding groove (622) extending vertically and penetrating therethrough; A first sliding block (63) is vertically slidably disposed in the first sliding groove (621); A first guide block (64) is located between the driving sleeve (62) and the linkage rod (52), and its outer wall is connected to the first sliding block (63); a downward end of the first guide block (64) has an inclined first slope (641) and a vertical first vertical surface (642); A second sliding block (65) is vertically slidably disposed in the second sliding groove (622); and A second guide block (66) is located between the driving sleeve (62) and the linkage rod (52), and its outer wall is connected to the second sliding block (65). An upward end of the second guide block (66) has an inclined second inclined surface (661) and a vertical second vertical surface (662); The driving block (61) can abut against the first inclined surface (641), the first vertical surface (642), the second inclined surface (661) and the second vertical surface (662); the first inclined surface (641) and the second inclined surface (661) have the same inclination direction relative to the sliding seat (41); and the first sliding block (63) and the second sliding block (65) tend to approach each other.

7. An embryo freezing and thawing machine as claimed in claim 6, characterized in that: The linkage assembly (60) further comprises: A first sliding ring (67), which is slidably sleeved outside the driving sleeve (62) and connected to an end of the first sliding block (63) away from the first guide block (64); a second sliding ring (68), which is slidably sleeved outside the driving sleeve (62) and connected to an end of the second sliding block (65) away from the second guide block (66); and The spring (69) is sleeved outside the driving sleeve (62), and its two ends are respectively against the first sliding ring (67) and the second sliding block.

8. An embryo freezing and thawing machine as claimed in claim 7, characterized in that: The driving mechanism (50) further comprises: A first wire drum (55) is coaxially connected to the rotating shaft (54) and has a wire groove formed on its outer circumference; A second wire drum (56) is coaxially connected to the rotating shaft (54) and has a wire groove formed on its outer circumference; A first pulley (57) rotatably disposed on the frame (20); a second pulley (58) rotatably disposed on the frame (20), wherein the second pulley (58) and the first pulley (57) are located on both sides of the rotating shaft (54); and A driving rope (59), one end of which is wound around the first wire drum (55), and the other end of which is wound around the first pulley (57) and the second pulley (58) in sequence and then connected to the second wire drum (56); The driving rope (59) outside the first wire drum (55) and the second wire drum (56) is ring-shaped; when the rotating shaft (54) rotates, one of the first wire drum (55) and the second wire drum (56) reels the driving rope (59) and the other releases the driving rope (59); the driving rope (59) outside the first wire drum (55) and the second wire drum (56) can remain stationary.

9. An embryo freezing and thawing machine as claimed in claim 8, characterized in that: The driving mechanism (50) further comprises: A first stopper (70) is arranged on one side of the first pulley (57) and is provided with a first limiting hole for the driving rope (59) to pass through; a second stopper (71), arranged on one side of the second pulley (58) and opposite to the first stopper (70), and having a first limiting hole for the driving rope (59) to pass through; and The stop block (73) is connected to the driving rope (59), is located between the first stop block (70) and the second stop block (71), and can be respectively abutted against the first stop block (70) and the second stop block (71).

10. An embryo freezing and thawing machine as claimed in claim 9, characterized in that: Two first magnetic blocks (80) are connected to the frame (20); the two first magnetic blocks (80) are located on both sides of the sliding seat (41); The two ends of the sliding seat (41) are respectively connected to second magnetic blocks (81); the second magnetic block (81) can be magnetically attracted to the first magnetic block (80) on the same side.

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