Multifunctional light reaction device and use method

Abstract

The invention discloses a multifunctional photoreaction device and a use method, and relates to the technical field of photoreaction. The multifunctional photoreaction device mainly comprises a reaction cavity for photoreaction, a light-emitting module for providing a light source for the photoreaction, a monitoring module for monitoring the photoreaction process and a control module for regulating and controlling reaction parameters. Wherein the light-emitting module can provide light sources with various wavelengths, and the light sources can be detached and replaced so as to adapt to different light source requirements; the monitoring module can be used for monitoring the photoreaction in the reaction cavity in real time; the control module can preset reaction parameters and adjust the reaction parameters in real time according to data fed back by the monitoring module. The bearable temperature of the reaction cavity in the device is from low temperature to medium temperature. The device can improve the reliability of the photoreaction and realize the accurate control of the photoreaction process. Due to the modular design, the device is good in stability, wide in application range and convenient to maintain in the later period, and the manufacturing and maintenance cost is reduced; and the portability of the device is improved due to the compact design.

Description

Technical Field

[0001] The present invention relates to the technical field of photoreaction devices, and particularly to a multifunctional photoreaction device with both reaction progress and process monitoring functions and a method of using the same. Background Art

[0002] In the sustainable development of modern society, efficient and clean energy utilization and environmental protection are major issues concerning the future of humanity. As a green and renewable energy utilization method, light-driven chemical reactions exhibit great application prospects. Through processes such as photocatalysis, water splitting for hydrogen production, and artificial photosynthesis, the direct conversion of light energy into chemical energy can be achieved, providing a clean energy source for industrial production while reducing the consumption of fossil fuels and greenhouse gas emissions. In addition, photoreactions also have extensive applications in fields such as organic synthesis, environmental remediation, and material preparation.

[0003] However, to truly industrialize the photocatalytic reaction technology, there are still many challenges. As the key equipment for realizing the conversion of light energy into chemical energy, the performance of the photoreaction device directly affects the reaction efficiency. Although there are already some photoreaction devices, the existing ones still have the following deficiencies: (1) Lack of in-situ real-time detection of the reaction system: The existing photoreaction devices generally lack effective means for real-time in-situ monitoring of the reaction process. These reactors rely on intermittent sampling and off-line analysis to obtain key parameters such as reactant concentration and product composition. Not only is the efficiency low, but it is also difficult to accurately control the reaction process due to untimely detection, which may lead to problems such as low substrate conversion rate or increased by-products. At the same time, limited by the complexity and volume of spectroscopic instruments, there are few successful precedents for integrating on-line spectroscopic detection units in existing photoreaction devices. Taking the cultivation of photosynthetic bacteria as an example, researchers usually need to regularly extract samples from the reactor and then use off-line instruments for detection and analysis. This intermittent and non-in-situ detection method is not only cumbersome to operate, but also may increase the risk of bacterial contamination due to frequent opening of the reaction chamber. (2) Insufficient ability to monitor and feedback-regulate the light source performance: The core of the photoreaction device is to drive the reaction process using light energy, and stable and reliable light energy output is the key to ensuring the smooth progress of the reaction. However, the existing devices often rely on manual adjustment of the light source, lacking real-time monitoring and automated feedback control mechanisms for key parameters such as light source power and spectrum. As a result, once the light source performance fluctuates during the reaction, such as light power attenuation or spectrum shift, it cannot be detected and corrected in time, making it difficult to ensure the consistency, stability of the photocatalytic reaction conditions and the repeatability of experimental results. (3) Insufficient parallel reaction ability and poor consistency: In industrial applications and research fields, it is usually necessary to conduct multiple parallel experiments under the same conditions to optimize reaction parameters and evaluate performance. However, limited by the structural design of the existing reactors and the lack of integrated process monitoring and feedback control, it is extremely challenging to accurately synchronously control key parameters such as light intensity and temperature when multiple reaction units are operating simultaneously. This limits the development of large-scale and high-throughput photosynthetic organism cultivation and photocatalytic reaction experiments, affecting the repeatability of experiments. (4) Inconvenient light source replacement and poor flexibility: Different photosynthetic organisms and photocatalytic reactions have specific and strict requirements for light wavelength. However, most of the reactors on the market at present are only equipped with light sources of fixed wavelengths, which are not detachable or require complex operations to disassemble the entire device when replacing the light source. This limitation seriously hinders the adaptability of the reactor to diverse reaction requirements, greatly limits its wide application in different experimental and application scenarios, and reduces the efficiency of experimental research and actual production.

[0004] In summary, there are still many deficiencies in the existing photoreaction devices in aspects such as real-time detection, light source regulation, batch reactions, and light source adaptability, making it difficult to meet the ever-changing needs of photoreaction research. The present invention precisely addresses the above problems and proposes a new type of multifunctional photoreaction device that is highly integrated and intelligent. Summary of the Invention

[0005] The object of the present invention is to provide a photoreaction device that can integrate reaction progress and process monitoring, and can flexibly regulate and perform real-time detection for different reaction doses and light source characteristics, thereby solving the problems mentioned in the background art.

[0006] On the one hand, the present invention provides a multifunctional photoreaction device, which at least includes: a receiving cavity 101, a reaction cavity 102, a monitoring module 103, a light-emitting module 104, a temperature control module 105, and a control module 106; wherein, the reaction cavity 102 is used for performing photoreactions, the light-emitting module 104 is used to provide a light source for the photoreaction, and the monitoring module 103 is used to perform real-time detection on the state signals during the photoreaction process.

[0007] Furthermore, the receiving cavity 101 is a structure that defines an internal space and houses internal modules, and the reaction cavity 102, the monitoring module 103, the light-emitting module 104, and the control module 106 are integrated within the receiving cavity 101.

[0008] Furthermore, the reaction cavity 102 at least includes one or more reaction chambers; the reaction chambers are used to place photoreaction containers; the light-emitting module 104 is used to provide a light source for the photoreaction; the temperature control module 105 is used to control the temperature of the reaction chambers and dissipate heat for the light-emitting module 104.

[0009] Furthermore, the monitoring module 103 is used to detect the state signals during the photoreaction process, which includes a first signal detector 6-2 and may also include a second signal detector 6-5. The state signals may be light source spectral parameters and / or light source intensity, etc.

[0010] Furthermore, the control module 106 is used to communicate and perform control adjustments on each module in the photoreaction device, such as the reaction cavity 102, the light-emitting module 104, and the temperature control module 105, according to the state signals detected by the monitoring module 103.

[0011] On the other hand, the present invention also provides a method for using a multifunctional photoreaction device, which includes:

[0012] According to the photoreaction to be performed, determine the size, structure, and quantity of the photoreaction containers in the reaction cavity 102; the photoreaction containers include reaction tubes 2 and reaction tube jackets 3.

[0013] Set the number of illuminated unit groups and light intensity required for the light reaction in the light-emitting module 104, and select the illumination light source with the corresponding wavelength according to different reaction media;

[0014] Start the light reaction device to carry out the light reaction; the monitoring module 103 detects the status signals during the reaction process in real time; at the same time, the control module 106 precisely regulates each module in the light reaction device according to the detection results of the status signals of the monitoring module 103.

[0015] Furthermore, it is detected that the motor 6-8 drives the overall detection system including the laser assembly 6-3 and the first signal detector 6-2 to rotate intermittently at a preset angle. When it stops rotating each time, the laser emitted by the laser assembly 6-3 passes through the reaction tube 2 in the reaction chamber 102, and the first signal detector 6-2 collects the optical signal transmitted through the reaction tube 2, and respectively realizes absorbance detection, discrete absorption spectrum detection or fluorescence spectrum detection according to different detection modes; for example, by analyzing the change in the intensity of the optical signal at a specific wavelength measured, the absorbance, absorption spectrum characteristics or fluorescence emission characteristics and other light absorption characteristics of the reactants in the reaction tube 2 can be calculated, and parameters such as the concentration of the reactants in the reaction tube 2 can be calculated, so as to monitor the concentration change and spectral dynamic characteristics of this path of reactants in real time;

[0016] Furthermore, when the monitoring module 103 is provided with a second signal detector, the second signal detector monitors the luminous power and spectral change of the reaction light source in real time. When it is detected that the power attenuation exceeds the preset threshold or the light source wavelength shifts, the control module 106 timely adjusts the illumination intensity or issues a warning prompt for replacing the light source to ensure the stability and reliability of the light source during the light reaction process.

[0017] In summary, due to the adoption of the above technical solutions, the beneficial effects of the present invention are:

[0018] The technical solution of the improved multifunctional photoreaction device of the present invention can achieve the full-process integration of reaction and detection, significantly reducing the complexity of manual operation and the risk of contamination; the uniquely improved rotating monitoring module integrates multi-parameter spectral detection functions such as absorbance, discrete absorption spectrum, and fluorescence spectrum, enabling real-time quantitative monitoring of the concentration of reactants / products in the system and dynamic analysis of the product types in the reaction system, providing a reliable basis for studying the mechanism of the photochemical reaction process and process optimization; through the uniquely improved light source life detection technology and precise spectral analysis technology, the wavelength change and attenuation of the light source are tracked in real time, warning of the degradation of the light source performance and ensuring the light quality stability of the photoreaction; the modular design innovation can flexibly adapt to different-capacity reaction tubes to meet diverse research needs; the light-emitting module is detachable and replaceable, and supports multi-wavelength light sources such as ultraviolet, visible light, and infrared, adapting to different photoreactions; the intelligent control system of the photoreaction device can precisely adjust the light intensity, stirring speed, and temperature, optimizing the reaction environment, and the parallel circuit design improves the system reliability and maintenance convenience. BRIEF DESCRIPTION OF THE DRAWINGS

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as limiting the scope. For those of ordinary skill in the art, without creative efforts, other related drawings can also be obtained based on these drawings, where:

[0020] Figure 1 is the system structure diagram of the multifunctional photoreaction device provided by the embodiment of the present invention;

[0021] Figure 2 is the front view of the multifunctional photoreaction device provided by the embodiment of the present invention;

[0022] Figure 3 is the right view of the multifunctional photoreaction device provided by the embodiment of the present invention;

[0023] Figure 4 is the top view of the multifunctional photoreaction device provided by the embodiment of the present invention;

[0024] Figure 5 is the bottom view of the multifunctional photoreaction device provided by the embodiment of the present invention;

[0025] Figure 6 is the perspective view of the multifunctional photoreaction device provided by the embodiment of the present invention;

[0026] Figure 7 is the half-sectional view of the multifunctional photoreaction device provided by the embodiment of the present invention;

[0027] Figure 8It is a right-side exploded view of the multi-functional photoreaction device provided by an embodiment of the present invention;

[0028] Figure 9 It is a left-side exploded view of the multi-functional photoreaction device provided by an embodiment of the present invention;

[0029] Figure 10 It is a core exploded view of the multi-functional photoreaction device provided by an embodiment of the present invention;

[0030] Figure 11 It is a front view of the monitoring module provided by an embodiment of the present invention;

[0031] Figure 12 It is a right view of the monitoring module provided by an embodiment of the present invention;

[0032] Figure 13 It is a top view of the monitoring module provided by an embodiment of the present invention;

[0033] Figure 14 It is a bottom view of the monitoring module provided by an embodiment of the present invention;

[0034] Figure 15 It is an exploded view of the monitoring module provided by an embodiment of the present invention;

[0035] Figure 16 It is an internal schematic diagram of the laser component of the monitoring module provided by an embodiment of the present invention;

[0036] Figure 17 It is a schematic diagram of the detection mode of the monitoring module provided by an embodiment of the present invention;

[0037] Figure 18 It is a schematic diagram of the control circuit of the multi-functional photoreaction device provided by an embodiment of the present invention;

[0038] Figure 19 It is a system structure diagram of the linear array photoreaction device provided by another embodiment of the present invention;

[0039] Figure 20 It is a reaction result diagram provided by an embodiment of the present invention;

[0040] Figure 21 It is another reaction result diagram provided by an embodiment of the present invention.

[0041] Explanation of reference numerals:

[0042] 100: Multi-functional photoreaction device; 101: Accommodating cavity; 102: Reaction cavity; 103: Monitoring module; 104: Light-emitting module; 105: Temperature control module; 106: Control module; 1: Outer shell; 2: Reaction tube; 3: Reaction tube jacket; 4: First cooling tube; 5: Reaction cavity body; 6: Photodetector; 7: Main board; 8: Stirring mechanism; 9: Glass baffle; 10: Lamp holder; 11: Main board base; 12: Heat sink; 13: Second cooling tube; 14: Motor positioner; 15: Photoreaction device base; 16: Upper cover plate of the outer shell; 17: Control system; 18: Bottom cover plate of the outer shell; 19: Photoreaction device light-shielding cover; 20: Light source disassembly baffle; 6-1: Motor outer shell; 6-2: First signal detector; 6-3: Laser assembly; 6-3-1: Laser; 6-3-2: Beam splitter prism; 6-4: Concave rocker arm; 6-5: Second signal detector; 6-6: Motor bottom cover; 6-7: Motor seat; 6-8: Detection motor; 6-9: Motor connecting shaft; 6-10: PCB disc type conductive slip ring; 6-11: Rocker arm connecting shaft; 8-1: Magnet; 8-2: Stirring motor; 17-1: PC industrial computer; 17-2: PWM module; 17-3: Voltage analog acquisition module; 17-4: DC24-17V buck module; 17-5: Thermistor temperature acquisition module; 17-6: DC24-12V buck module; 200: Linear array photoreaction device; 201: Linear array temperature control module; 202: Linear array reaction cavity; 203: Linear array monitoring module; 204: Linear sliding guide; 205: Linear array light-emitting module; 206: Linear array stirring module. Detailed implementation manners

[0043] In order to make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are only used to explain the present invention and are not used to limit the present invention, that is, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Generally, the components of the embodiments of the present invention described and shown in the drawings here can be arranged and designed in various different configurations.

[0044] It should be noted that relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the terms "comprising", "including" or any other variant thereof are intended to cover non-exclusive inclusion, such that a process, method, article or device comprising a series of elements not only includes those elements, but also includes other elements not expressly listed, or elements inherent to such process, method, article or device. Without further limitation, an element defined by the statement "comprising..." does not exclude the presence of additional identical elements in the process, method, article or device comprising the element.

[0045] The photoreaction device described in the present invention can be used as any photoreaction device.

[0046] The photoreaction described in the present invention refers to a photochemical reaction, including any chemical reaction involving a light source. For example, the photoreaction described in the present invention may include a photocatalytic reaction or a photo-triggered reaction, etc.

[0047] The photocatalytic reaction described in the present invention may, for example, refer to a reaction in which, under the irradiation of light, a photocatalyst absorbs light energy, the excited-state photocatalyst directly reacts with a substrate, and finally the photocatalyst returns to the ground state, and the substrate generates a product through the action of the photocatalyst. In this process, the photocatalyst is neither consumed nor increased and finally remains unchanged.

[0048] The photo-triggered reaction described in the present invention may, for example, refer to the generation of radical intermediates / ionic intermediates by a photoinitiator absorbing light, and these intermediates further react with a substrate to generate new radicals / ions, thereby initiating a radical / ionic chain reaction.

[0049] The photochemical reaction of the present invention includes photoreactions occurring in vitro or in vivo, such as photocatalytic or photo-triggered reactions occurring in vitro or in vivo.

[0050] In some specific embodiments, the photoreaction includes photochemical reactions (or photocatalytic, photo-triggered reactions, etc.) in the fields of organic synthesis, environmental chemistry, materials science, semiconductors or biology.

[0051] In some specific embodiments, the photoreaction may include photooxidation reaction, photoreduction reaction, photodisplacement reaction, photopolymerization reaction, photocoupling reaction, photodegradation reaction, photo-oxygen-microbial reaction, photosynthesis reaction, photorespiration, photoinduction, photofermentation, photorepair or phototranscription, etc.

[0052] The features and properties of the present invention will be further described in detail below in conjunction with examples.

[0053] Example 1

[0054] As shown Figures 1 to 10 in the figure, this embodiment provides a multifunctional photoreaction device 100. As shown Figure 1 in the figure, the photoreaction device 100 at least includes a containing cavity 101, a reaction cavity 102, a monitoring module 103, a light-emitting module 104, a temperature control module 105, and a control module 106.

[0055] The containing cavity 101 is a structure that defines an internal space and houses internal modules, and at least includes a housing 1, a housing top cover plate 16, and a housing bottom cover plate 18. Each module such as the reaction cavity 102, the monitoring module 103, the light-emitting module 104, the temperature control module 105, and the control module 106 is integrated in the containing cavity 101.

[0056] The housing 1 is connected to the housing bottom cover plate 18 and the housing top cover plate 16. As a preferred embodiment, the housing 1 is made of a light-shielding and heat-insulating material, and a photoreaction device light-shielding cover 19 can be placed above it to avoid light pollution during the photoreaction process and reduce the harm to the eyes of equipment operators.

[0057] In some embodiments, the containing cavity 101 may further include a main board base 11 and a main board 7 fixed on the photoreaction device base 15, etc. The main board base 11 is used to fix the main board 7 above it, and the power supply circuit led out from the main board 7 can be used to supply power to each module inside the photoreaction device.

[0058] The reaction cavity 102 is used for carrying out photoreactions, and at least includes a plurality of reaction chambers and a stirring mechanism 8. The reaction chambers are used to place photoreaction containers.

[0059] In a preferred embodiment, the reaction chambers in the reaction cavity 102 are arranged in a ring in the containing cavity 101. At the same time, 2 - 12 or more reaction chambers can be set in the reaction cavity 102 according to actual needs. The adjustability of the number of channels provides a more robust platform for photoreaction research.

[0060] The stirring mechanism 8 is used to provide a stirring driving force for the photoreaction solution. As a preferred embodiment, the stirring mechanism 8 is a magnetic stirring mechanism, which is arranged at a position slightly below the middle of the ring formed by each reaction chamber and is distributed vertically with the monitoring module. The magnetic stirring mechanism includes a stirring component and a magnet component. The magnet component includes a coupling and a magnet arranged at the end of the coupling. The coupling is connected to the output shaft of the stirring component.

[0061] The monitoring module 103 is used to detect the state parameter signals during the photoreaction process. The state parameter signals at least include light source spectrum, light source intensity, reactant concentration, etc.

[0062] The light-emitting module 104 is used to provide an illumination light source for the photoreaction. The light-emitting module 104 includes multiple lighting units arranged around a plurality of reaction chambers. The lighting units are designed as a parallel circuit, and each lighting unit can be individually controlled and detachably installed, avoiding mutual interference in case of faults and facilitating maintenance and use. In some embodiments, the illumination light source can be turned off, and at this time, the photoreaction device can also be used for non-photoreaction.

[0063] As a preferred embodiment, the lighting unit is a light-emitting component corresponding to each reaction chamber one by one. Each group of lighting units includes a lamp group and a detachable lamp holder 10. Each lamp group is fixed in the fixing groove of the lamp holder 10. The lamp holder 10 is arranged below the main board base 11 corresponding to the reaction chamber. Thus, the lamp holder 10 is electrically connected to the main board 7, thereby realizing the power supply of the light source. In a preferred embodiment, the lamp holder 10 is inserted into the card slot of the main board 7 to achieve electrical connection with the main board 7, and each lamp group is electrically independent of each other. In some other embodiments, a detachable light source removal baffle 20 can be provided at the lamp holder 10, which is magnetically attracted to the outer shell 1, and the lamp group can be replaced without disassembling the reactor, which is more rapid and convenient to operate.

[0064] In some embodiments, the light source of the lamp group can be selected from one or more of ultraviolet lamps, visible light lamps, infrared lamps, fluorescent lamps, LED lamps, mercury lamps, and xenon lamps.

[0065] The temperature control module 105 is used to control the temperature of the reaction chamber and dissipate heat for the light-emitting module 104. The temperature control module 105 includes two parts: a reaction chamber temperature control unit and a light source heat dissipation unit. The reaction chamber temperature control unit is arranged on the periphery of the reaction chamber, and it includes a first cooling pipe 4 coiled around the periphery of the reaction cavity 5. The light source heat dissipation unit is arranged below the lamp holder 10, and a heat dissipation plate 12 is provided in close contact with the lamp holder 10. The second cooling pipe 13 is coiled inside the heat dissipation plate 12. As a preferred embodiment, the lamp holder 10 and the heat dissipation plate 12 are made of aluminum material with good heat conduction performance. Specific cooling methods can adopt different methods.

[0066] As a preferred embodiment, in order to precisely control the temperature environment inside the photoreaction device, the temperature control module 105 adopts two different temperature control methods, which can be switched according to the level of the reaction temperature. When the target reaction temperature is within the range of 0 - 100 °C, the temperature control module 105 can select pure water circulation temperature control. By using the integrated temperature sensor to monitor the reaction solution temperature in real time and combining with the control algorithm, the temperature control module 105 automatically adjusts the temperature and flow rate of the circulating water flow to ensure that the reaction system proceeds stably at the set temperature. When the reaction temperature is higher than 100 °C, the temperature control module 105 can select high-temperature oil circulation temperature control. Similar to the water circulation system, the oil circulation temperature control also adopts closed-loop control, dynamically adjusting the oil temperature and flow rate according to the temperature feedback to keep the reaction temperature stable at the set value. In addition, the temperature control module 105 is isolated from other modules by using low-thermal-conductivity materials, minimizing the heat exchange inside the system and improving the accuracy of temperature control.

[0067] The control module 106 is used to control the reaction chamber 102, the monitoring module 103, the light-emitting module 104, the temperature control module 105, etc. For example, the control module 106 can adjust the stirring speed of the stirring mechanism 8.

[0068] Specifically, the control module 106 can perform data interaction with each module through wired or wireless communication methods, and perform real-time regulation on each module in the photoreaction device according to the status signals detected by the monitoring module 103. The communication methods include but are not limited to serial communication, wireless communication, or other industrial standard protocols.

[0069] In some embodiments, the control module 106 can communicate with other modules through wired connection or wireless connection.

[0070] Embodiment 2

[0071] As Figures 2 to 10 shown, in the embodiment of the present invention, the structure and functions of the reaction chamber 102 are described one by one with an 8-way reaction chamber as an example.

[0072] In the reaction chamber 102, 8 reaction chambers are evenly arranged in a ring in the cylindrical reaction cavity 5. Each reaction chamber is a hollow chamber, and it can place a photoreaction container composed of a reaction tube 2 and a reaction tube jacket 3. The reaction tube jacket 3 is used to fix the reaction tube 2, and multiple reaction tube jackets 3 can have the same outer diameter size and different inner diameter sizes. When the reaction tube 2 is combined with the reaction tube jacket 3 with the corresponding inner diameter size, it can be inserted into the corresponding reaction chamber. The reaction cavity 5 is connected to the main board base 11. The bottom of each reaction chamber in the reaction cavity 5 is blocked by a glass baffle 9 to prevent sundries from falling and damaging the light source.

[0073] In a preferred embodiment, the reaction tube jacket 3 can be custom-designed with different inner diameter sizes, for example, to accommodate reaction tubes 2 with different capacities such as the commonly used 5 ml, 10 ml, 15 ml, 20 ml, 25 ml, 50 ml, etc. Moreover, the size and shape of the reaction tube jacket 3 can be customized to meet more capacity requirements. For example, bioreaction tubes suitable for biological culture and Schlenk tubes suitable for chemical reactions.

[0074] As Figure 10 shown, the stirring mechanism 8 includes a magnet 8-1 and a stirring motor 8-2. The magnet 8-1 is arranged at the annular center position inside the reaction cavity 5. One end of the transmission shaft of the stirring motor 8-2 passes through the central circular holes of the heat dissipation plate 12 and the motor locator 14, and is fixed to the bottom surface of the motor locator 14 by screws. At the same time, the motor locator 14 is fixed on the photoreaction device base 15; the other end of the transmission shaft is connected to the magnet 8-1. After the stirring motor 8-2 is powered on, the stirring motor 8-2 is adjusted and rotated by the control module 106, driving the magnet 8-1 to rotate, and then driving the magnetic stirrer in the reaction tube 2 to rotate, so that the photoreaction solution or culture solution in the reaction tube 2 can be evenly and stably distributed.

[0075] As a preferred embodiment, the magnet 8-1 is made of rubidium iron.

[0076] Embodiment III

[0077] The embodiment of the present invention details the structure and function of the monitoring module 103 in the aforementioned photoreaction device.

[0078] The monitoring module 103 is the core improved structure of the photoreaction device in this embodiment, and is used to detect the state parameter signals during the photoreaction process. The improved monitoring module 103 in the embodiment of the present invention can skillfully solve the problems of continuous and accurate detection of multi-channel reaction tubes by adopting a rotation detection mechanism.

[0079] The monitoring module 103 at least includes a photodetector 6. Further, the photodetector 6 at least includes a first signal detector 6-2 and / or a second signal detector 6-5. The first signal detector 6-2 is used to detect the optical signal parameters after passing through the reaction solution, and the second signal detector 6-5 is used to detect the optical signal parameters of the illumination light source.

[0080] Taking the first signal detector 6-2 as a micro spectrometer and the second signal detector 6-5 as a micro spectrometer as a preferred embodiment for illustration, as Figures 11 to 15As shown in the figure, the optical detector 6 includes a motor housing 6-1, a first signal detector 6-2, a laser assembly 6-3, a concave rocker arm 6-4, a second signal detector 6-5, a motor bottom cover 6-6, a motor base 6-7, a detection motor 6-8, a motor connecting shaft 6-9, a PCB disc conductive slip ring 6-10, and a rocker arm connecting shaft 6-11.

[0081] The internal structure of the laser assembly 6-3 is as Figure 16 shown. The laser assembly 6-3 consists of a series of small lasers 6-3-1 and beam splitting prisms 6-3-2. Each laser 6-3-1 emits a different wavelength. The laser beams emitted by these independent lasers 6-3-1 are combined into a group of coaxial output beams through a series of beam splitting prisms 6-3-2 arranged in sequence along the optical path. The laser wavelength range of the lasers 6-3-1 includes multiple key wavelengths from the ultraviolet, visible light to the infrared light bands, which are used to meet the specific requirements of different reactants for the detection wavelength. When detecting specific reaction parameters, the number of lasers to be turned on and the operating wavelength can be flexibly selected through the control module 106. For example, a single laser with a specific wavelength can be turned on alone for single-wavelength detection, or multiple lasers with specific wavelengths can be turned on in sequence to obtain discrete spectral information. The beam splitting prisms 6-3-2 in the laser assembly 6-3 are arranged in sequence according to the wavelength from long to short, and are used to gradually adjust the laser beams with different wavelengths into a group of coaxial output light. The first prism is located near the laser with the longest wavelength (such as a red light laser). This prism is designed to only reflect the beam of this longest wavelength and guide it to the output end. The second prism is located in front of the first prism, and its function is to reflect the laser beam of the second longest wavelength (such as yellow light) and coaxially align it with the beam of the longest wavelength (red light) reflected by the first prism. At the same time, this prism transmits all the beams with wavelengths longer than yellow light (such as red light). And so on, each prism reflects a laser beam of a specific wavelength and transmits all the beams with wavelengths longer than it. Through this arrangement, the laser beams with different wavelengths are gradually reflected and superimposed, and finally form a group of discrete wavelength laser beams propagating along the same optical axis. By controlling the number of lasers 6-3-1 with specific wavelengths in the laser assembly 6-3 through the control module 106, the measurement of the absorbance of a single wavelength and the measurement of the discrete absorption spectrum of multiple wavelengths can be achieved.

[0082] The PCB disc conductive slip ring 6-10 is composed of a slip ring stator and a slip ring rotor: through the PCB disc conductive slip ring 6-10, the circuits of the laser assembly 6-3, the first signal detector 6-2, and the second signal detector 6-5 are connected to the control module 106, preventing the wire winding caused by the rotation of the concave rocker arm 6-4. This design effectively solves the technical problem of wire winding in traditional rotary detection and significantly improves the stability and reliability of signal transmission.

[0083] Specifically, after the detection motor 6-8 is encapsulated by the motor housing 6-1, the motor base 6-7, and the bottom cover 6-6, it is fixed in the central through-hole of the reaction chamber 5 by screws. The diameter of the central through-hole of the reaction chamber 5 is the same as that of the motor housing 6-1. At the same time, the diameter of the motor base 6-7 is slightly larger than the diameter of the central through-hole, so that the motor base 6-7 is connected to the central through-hole of the reaction chamber 5. Preferably, the motor base 6-7 is snap-fitted into the circular card slot above the central through-hole of the reaction chamber 5. The rocker connecting shaft 6-11 is connected to the motor connecting shaft 6-9 inside the detection motor 6-8, and the other end of the rocker connecting shaft 6-11 is connected to the concave rocker 6-4. Thus, the rotation of the detection motor 6-8 can drive the concave rocker 6-4 to rotate. The first signal detector 6-2 and the laser assembly 6-3 are respectively arranged in the card slots at both ends of the concave rocker 6-4, and the second signal detector 6-5 is fixed at the center of the bottom rod of the concave rocker 6-4. The slip ring stator of the PCB disc type conductive slip ring 6-10 is fixed at the upper end of the motor base 6-7, and the slip ring rotor is fixed at the cover of the rocker connecting shaft 6-11.

[0084] After the detection motor 6-8 is powered on, the detection motor 6-8 drives the detection assembly including the laser assembly 6-3, the first signal detector 6-2, and the concave rocker 6-4 to perform precise intermittent rotation. As Figure 17 shown in the schematic diagram of the detection mode, at this time, two different detection modes can be flexibly switched through the control module 106: detection mode A (absorbance / discrete absorption spectrum measurement) and detection mode B (fluorescence spectrum measurement). Among them, detection mode A is the default basic detection mode. In detection mode A, when the detection assembly rotates to the center of a certain reaction tube 2, the detection motor 6-8 pauses. At this time, the laser emitted by the laser assembly 6-3 passes through the reaction tube 2, and the first signal detector 6-2 (micro spectrometer) collects the spectral signals transmitted through the reaction tube 2. According to needs, the control module 106 can adjust the number of lasers 6-3-1 turned on to switch between single-wavelength absorbance measurement and multi-wavelength discrete absorption spectrum measurement. If the laser outputs a single wavelength of laser, the absorbance of the reactants in the reaction tube 2 is calculated by measuring the intensity of the optical signal at a specific wavelength, and then the concentration of the reactants in the reaction tube 2 is deduced to achieve real-time concentration detection. This method is mainly applicable to the real-time concentration detection in the photosynthetic organism culture process. By controlling the emission wavelength of the laser, the concentration changes of different target substances can be selectively measured; if the laser sequentially outputs multiple wavelengths of laser, the first signal detector 6-2 (micro spectrometer) respectively receives the transmitted light intensity signals at each wavelength to form a discrete absorption spectrum. By analyzing the dynamic changes of the absorbance at each wavelength, the reaction process can be monitored in real time, and the relative content changes of reactants, intermediate products, and products can be obtained, providing a key basis for studying the reaction mechanism.

[0085] In detection mode A, the luminous power of the laser component 6-3 is controlled at a low level to avoid saturation or damage of the first signal detector 6-2 caused by high-intensity incident light. In detection mode B, the detection motor 6-8 rotates the concave rocker arm 6-4 to a specific angle, so that the laser beam is obliquely incident on the reaction solution at a certain angle deviating from the center of the reaction tube, to avoid direct incidence of the laser beam and being received by the first signal detector 6-2 (micro spectrometer) resulting in saturation or damage. When the excitation beam irradiates the reaction solution, part of the absorbed energy will be emitted in the form of fluorescence in all directions. The first signal detector 6-2 (micro spectrometer) receives the fluorescence signal to obtain the fluorescence spectrum. It should be noted that the detected fluorescence spectrum may be superimposed with a small amount of scattered light background, mainly due to the interaction between the reaction solution molecules and the incident light (such as the Tyndall effect). In this regard, the original spectral data collected can be subjected to background subtraction and normalization processing through software algorithms to finally obtain a pure spectrum reflecting the fluorescence characteristics of the reaction system. In detection mode B, due to the weakness of the fluorescence signal, the laser component 6-3 operates at a higher power level to excite fluorescence generation. However, if the too strong laser is received by the micro spectrometer, it will cause saturation or damage. Therefore, the detection motor 6-8 rotates the concave rocker arm 6-4 to a specific angle, so that the laser beam is obliquely incident on the reaction solution at a certain angle deviating from the center of the reaction tube.

[0086] As a preferred embodiment, the value of the certain angle at which the laser beam deviates from the center of the reaction tube is determined by the inner diameter of the reaction tube (2) and the distance from the rotation center to the center of the reaction tube (2), and its range is preferably 3° to 8° or more. Further, for a reaction tube with an inner diameter of 15 mm, the deviation angle is preferably 5°; for a reaction tube with an inner diameter of 18 mm, the deviation angle is preferably 6°; for a reaction tube with an inner diameter of 24 mm, the deviation angle is preferably 7°. Through the precision control of the detection motor (6-8), the deviation angle requirements of reaction tubes of different sizes can be adapted to ensure that the laser beam effectively enters the reaction solution and does not directly enter the first signal detector (6-2).

[0087] In the detection mode B (fluorescence spectroscopy measurement), the improved design of the present invention has significant advantages compared with the traditional fluorescence spectroscopy detection method. Conventional fluorescence spectroscopy detection usually requires multiple sets of filters to be configured in front of the detector to separate the excitation light and the emission light, so as to ensure the accurate detection of the fluorescence signal. However, this design requires a complex filter switching mechanism, which not only increases the structural complexity and manufacturing cost of the device, but also limits the miniaturization and portability of the equipment. The improved solution of the present invention innovatively biases the laser beam at a certain angle into the reaction solution, and utilizes the reflection and refraction characteristics of light to make the excitation light not directly enter the spectrometer, thus effectively avoiding the interference of the excitation light on the detection of the fluorescence signal. This design does not require the use of filters and their switching mechanisms, significantly simplifies the system structure, and reduces the manufacturing and maintenance costs. In addition, this design also realizes the miniaturization and integration of the fluorescence spectroscopy detection module, making it more suitable for high-throughput and automated photochemical reaction monitoring scenarios, and providing an efficient and economical technical solution for photosynthetic organism cultivation and photochemical reaction research.

[0088] By switching between the two modes, the monitoring module can easily adapt to the monitoring requirements of different reaction systems. Since optical components such as the laser and the micro spectrometer are fixed on the concave swing arm, when the concave swing arm rotates under the drive of the detection motor, the switching between the two detection modes can be achieved only by controlling the luminous power and the incident angle of the laser component 6-3 through the control module 106, without adjusting the reaction hardware, which greatly improves the convenience and automation degree of the detection process. Compared with the traditional photochemical reaction device that relies on off-line analysis, this embodiment realizes the on-line detection of multiple parameters such as absorbance, discrete absorption spectrum, and fluorescence emission spectrum by the highly integrated laser light source and micro spectrometer, and can comprehensively evaluate the starting materials, reaction process, and generated products of the photochemical reaction, and has wide applicability in different types of photochemical reaction research.

[0089] On the other hand, in the detection mode A (detection mode A is the default basic detection mode), when the detection component rotates to the center of a certain reaction tube 2, the detection motor 6-8 pauses, and at this time, the second signal detector 6-5 (micro spectrometer) is exactly corresponding to the center of the illumination light source, receives the illumination light source and performs light source spectrum detection and light intensity detection on the illumination light source. The control module 106 can realize the automatic regulation of the illumination light source according to the detection results.

[0090] After the detection is completed, the detection motor 6-8 continues to rotate to the next reaction tube 2 and performs the same detection on this reaction tube 2.

[0091] In a preferred embodiment, the micro spectrometer uses a Hamamatsu micro spectrometer, model C12880MA. The detected light source enters the entrance slit of the micro spectrometer, is separated by a grating beam splitter, and is received by a CMOS linear sensor. The signal is converted by a drive circuit to obtain the spectrum and light intensity values of the illumination light source, facilitating the regulation of the illumination light source.

[0092] In some embodiments, the first signal detector 6-2 and the second signal detector 6-5 are both set as micro spectrometers, which can respectively detect the spectrum / light intensity of the light source after passing through the reaction solution and the illumination light source. In other embodiments, to reduce costs, the second signal detector 6-5 can be replaced with a photodetector, that is, only the light intensity of the illumination light source is detected. In still other embodiments, to further reduce costs, both the first signal detector 6-2 and the second signal detector 6-5 can be replaced with photodetectors, that is, only the light intensity of the light source after passing through the reaction solution and the illumination light source is detected.

[0093] Embodiment 4

[0094] In this embodiment, the first signal detector 6-2 is a micro spectrometer and the second signal detector 6-5 is a micro spectrometer, and the usage method of the aforementioned multifunctional photoreaction device will be described in detail.

[0095] Before using the multifunctional photoreaction device, first select a reaction tube jacket 3 with a corresponding inner diameter size according to the size of the reaction tube 2 to be used, which can flexibly adapt to various reaction tubes 2 of different sizes, improving the adaptability of the photoreaction device.

[0096] Then, the reaction tube 2 is sleeved into the reaction tube jacket 3 and inserted into the corresponding reaction chamber in sequence. The reaction tube 2 contains a photoreaction solution and a magnetic stirrer. After being powered on, the stirring motor 8-2 rotates, driving the magnet 8-1 to rotate, and then driving the magnetic stirrer in the reaction tube 2 to rotate, realizing uniform stirring of the reaction medium in the reaction tube 2.

[0097] Set the number of groups of illuminated units to be turned on and the light intensity required for reaction initialization through the control module 106, and select an illumination light source with a corresponding wavelength according to different reaction media.

[0098] As a preferred embodiment, an improved rotation detection mechanism is adopted to detect the reaction state during the reaction or cultivation process. On the one hand, the detection motor 6-8 drives the overall detection system including the laser component 6-3 and the first signal detector 6-2 to rotate intermittently at a preset angle. The setting of the preset angle preferably satisfies the following functions: when staying at each rotation, the laser emitted by the laser component 6-3 passes through the reaction tube 2 in the reaction chamber 102, and the first signal detector 6-2 collects the spectral signal transmitted through the reaction tube 2. The absorbance of the reactants in the reaction tube 2 is calculated by measuring the intensity of the optical signal at a specific wavelength, and then the concentration of the reactants in the reaction tube 2 is calculated to monitor the concentration change and spectral information of this path of reactants in real time.

[0099] Specifically, after measuring and obtaining the laser light intensity transmitted through the reaction tube 2 by the first signal detector 6-2, the absorbance of the reactants in the reaction tube 2 is calculated and obtained, and the corresponding concentration of the reactants in each reaction tube 2 can be accurately calculated through a pre-established standard curve. The improved rotation detection mechanism of the photoreaction device not only realizes continuous and rapid detection of multiple-channel reaction tubes during the reaction, but also significantly reduces manual intervention and improves the accuracy and automation of detection.

[0100] As another preferred embodiment, it is also possible to simultaneously set that during the use of the light source, the second signal detector 6-5 located at the center of the concave rocker arm 6-4 monitors the luminous power and spectral changes of the illumination light source in real time.

[0101] The control module 106 can flexibly adjust the control parameters of the photoreaction device according to the signal detection results of the first signal detector 6-2 and / or the second signal detector 6-5, including light intensity, stirring speed, temperature, etc., to ensure that the photoreactants react under the optimal reaction conditions. For example, when it is detected that the wavelength of the light source deviates or the power attenuation exceeds the preset threshold, the control module 106 will adjust the light source parameters or issue a warning to replace the light source in a timely manner. The light source parameters include but are not limited to light intensity, light wavelength, etc., to ensure the stability and reliability of the illumination light source during the photoreaction process. In a specific embodiment, when it is detected that the luminous power of the illumination light source is lower than the power of the set threshold, the control module 106 adjusts the duty cycle of the control signal through pulse width modulation to increase the light output intensity of the illumination light source.

[0102] Embodiment Five

[0103] This embodiment further elaborates on the structure and function of the control module 106 in the aforementioned multifunctional photoreaction device. As Figures 2 to 9As shown in the figure, the control module 106 of the present invention mainly includes a control system 17. Further, the control system 17 at least includes main modules such as a PC industrial control computer 17-1, a PWM module 17-2, a voltage analog acquisition module 17-3, a DC24-17V step-down module 17-4, a thermistor temperature acquisition module 17-5, a DC24-12V step-down module 17-6, etc., as well as some circuit components.

[0104] By connecting an external power adapter (AC220V-DC24V), a stable and reliable working voltage can be provided for the entire photoreaction device. On this basis, the photoreaction device also has two-stage DC-DC step-down circuits built-in: one is the DC24-17V step-down module 17-4, which is used to step down 24V to 17V to drive the LED lighting source; the other is the DC24-12V step-down module 17-6, which is used to step down 24V to 12V to provide power for the PC industrial control computer 17-1. The design of the multi-stage step-down circuit effectively distributes the system power supply, minimizing power consumption while ensuring the working voltage of each module.

[0105] The PC industrial control computer 17-1 is the core of the entire control system. By running the self-developed supporting monitoring software, it can realize the intelligent management of the reaction process. The PC industrial control computer 17-1 conducts data interaction with each functional module through a communication interface, and adjusts parameters such as the light source brightness, the temperature of the reaction solution, the stirring speed, and the detection timing in real time according to the collected signals and the preset control strategies, and records and displays the reaction state curve, providing an efficient and convenient human-machine interaction interface for users. Among them, the control functions related to the monitoring module 103 mainly include two aspects: one is to switch between absorbance / discrete absorption spectrum measurement (detection mode A) and fluorescence spectrum measurement (detection mode B); the other is to adjust the working parameters of the laser component 6-3.

[0106] The PC industrial control computer 17-1 realizes the real-time monitoring and automatic feedback control of the lighting source through a control program. Specifically, the program reads the measurement data of the second signal detector 6-5 in real time to obtain the light intensity and spectral information of the lighting source. When the light intensity is different from the set threshold, the program immediately executes the light intensity feedback algorithm, calculates the compensation power required for the light source, and transmits the control signal to the PWM module 17-2 to adjust the light source output to ensure that the photoreaction proceeds under constant and stable lighting conditions. If the program detects an abnormal shift in the emission spectrum of the light source, it will promptly issue a warning to the user to remind them to replace the light source in time to avoid the influence of the deterioration of the light source performance on the experimental results.

[0107] The PWM module 17-2 is used to convert the control instructions issued by the PC industrial control computer 17-1 into PWM waveforms with corresponding duty cycles. On the one hand, it controls the brightness of the LED light source, the brightness of the laser, and the number of lasers turned on through the LED drive module. On the other hand, it directly controls the rotation speeds of the stirring motor 8-2 and the detection motor 6-8. The PWM module 17-2 includes independent multi-channel PWM outputs, enabling each load to be adjusted independently to meet the specific requirements for lighting and stirring at different reaction stages. The control program sends signal instructions to the PWM module 17-2 according to experimental needs to control the laser power and the number of lasers turned on inside the laser component 6-3.

[0108] The voltage analog quantity acquisition module 17-3 and the thermistor temperature acquisition module 17-5 are used to convert analog quantities such as the concentration and temperature of the reactants into digital signals for the industrial control computer to analyze and process. Among them, the concentration signal comes from the first signal detector 6-2. The IV amplifier amplifies the weak current into a 0-5V voltage signal and then inputs it into the voltage analog quantity acquisition module 17-3 for analog-to-digital conversion. The temperature signal comes from the thermistor and is also converted into a digital signal after being matched by the signal conditioning circuit.

[0109] As Figure 18 shown, taking the industrial control computer as the PC industrial control computer 17-1 and the LED as the lighting source as an example, through running the independently developed control program, the intelligent monitoring and adjustment control of the photoreaction device are realized. The control program conducts two-way data transmission with the voltage analog quantity acquisition module 17-3 and the PWM module 17-2 in the lower computer circuit through the USB to RS485 communication interface.

[0110] In a preferred embodiment, when both the first signal detector 6-2 and the second signal detector 6-5 are photodetectors, the voltage analog quantity acquisition module 17-3 is used to collect the voltage values of the output signals of the photodetectors in real time. It is connected to the two photodetectors through two IV amplifier circuits, respectively used to receive the laser transmitted light signal and the LED reaction light signal. The IV amplifier can amplify and convert the weak photocurrent output by the photodetector into a 0-5V voltage signal for the voltage analog quantity acquisition module 17-3 to perform analog-to-digital conversion. The converted digital quantity is uploaded to the control program through the RS485 interface for drawing the real-time concentration curve and executing the concentration threshold alarm.

[0111] The PWM module 17-2 contains multiple independent control channels, which receive the brightness / speed setting values ​​from the control program and convert them into PWM waveforms with corresponding duty cycles: on the one hand, the luminous intensity of the 8 LED light sources is adjusted through the LED constant current drive module; on the other hand, the motors of the stirring mechanism 8 and the light detector 6 are directly driven to control their speeds. In a preferred embodiment, the light detector 6 uses a servo motor, which can achieve precise intermittent angular displacement control through the frequency and duty cycle of the PWM square wave, and cooperate with the encoder provided by the servo motor for position feedback, which significantly improves the repeatability of the detection action. The LED light source and laser component 6-3 respectively achieve continuous adjustable brightness of 0-100% through the duty cycle of the PWM signal.

[0112] When the light reaction device starts working, the upper computer software generates corresponding control instructions according to the reaction parameters set by the user (such as light intensity, temperature, stirring speed, etc.), and sends them to the lower computer control circuit through the USB-RS485 interface. The multi-channel PWM module 17-2 generates multiple PWM waveforms according to the received instructions to control the LED drive circuit and the motor drive circuit respectively.

[0113] The LED driving circuit converts the PWM signal into a constant current driving signal to adjust the brightness of the LED light source and the laser assembly 6-3. The driving circuits of the detection motor 6-8 and the stirring motor 8-2 control the rotation of the motors according to their respective PWM signals.

[0114] At the same time, the reaction solution absorbs and scatters the laser beam, and the transmitted light through the reaction solution is received by the first signal detector 6-2 and converted into a weak current signal. The IV amplifier amplifies the weak current and converts it into a voltage signal for the voltage acquisition module to convert. The voltage acquisition module digitizes the output voltage signal and uploads it to the host computer. The host computer software calculates the real-time concentration of the reaction solution or culture solution based on the collected voltage value and the pre-calibrated concentration-voltage curve, and draws the concentration-time curve in real time on the interface.

[0115] During the continuous operation of the photoreaction device, the host computer software adjusts the LED light source intensity, stirring speed and other parameters in real time according to the set control strategy and the collected concentration, temperature and other data to achieve closed-loop optimization control of the reaction or culture process. The above process is continuously cycled until the preset reaction time or reaction concentration is reached.

[0116] In summary, the control system makes full use of the modular hardware design and the combination of software and hardware of computer technology to achieve the automatic monitoring and intelligent regulation of the photoreaction process. Compared with manual operation, this system can significantly improve the reaction efficiency and improve the consistency and repeatability of the reaction effect. At the same time, the PC industrial control computer 17-1 and the host computer software provide a friendly human-computer interaction interface, enabling experimental personnel to conveniently set various reaction parameters and real-time monitor the state curve of the reaction process, greatly reducing the burden of manual operation.

[0117] Example Six

[0118] As Figure 19 shown in another preferred embodiment, specifically, a linear array photoreaction device 200. The linear array photoreaction device 200 includes a linear array temperature control module 201, a linear array reaction chamber module 202, a linear array monitoring module 203, a linear sliding guide 204, a linear array light-emitting module 205, and a linear array stirring module 206. Different from the annular reaction chamber arrangement structure in the previous embodiment, a plurality of reaction chambers in the linear array photoreaction device 200 are arranged in a linear array in a rectangular accommodating cavity, further expanding the movement mode of the linear array monitoring module 203 to adapt to different experimental requirements. Correspondingly, the linear array monitoring module 203 is arranged on one side of the reaction chamber array and can reciprocate along the linear sliding guide 204 parallel to the reaction chamber array. The remaining structures of the linear array photoreaction device 200, such as the configuration of the reaction chamber, the light-emitting module, the temperature control module, and the control system, are basically the same as those in the previous annular photoreaction device embodiment.

[0119] Specifically, the linear array monitoring module 203 is installed on the linear sliding guide 204 through a slide rail. The linear array monitoring module 203 integrates a laser, a micro spectrometer, and a control circuit, etc. During operation, the control circuit drives the linear array detection module 203 to reciprocate along the linear sliding guide 204 and sequentially performs concentration or spectral detection on the reaction solutions in each reaction chamber. When the linear array detection module 203 moves to the central position of a certain reaction chamber, the laser beam emitted by the laser passes through the reaction solution in the reaction chamber. At the same time, the micro spectrometer receives the laser signal transmitted through the reaction solution and transmits the detection signal to the control circuit for analysis and calculation. By measuring the light intensity of the transmitted laser, the absorbance is calculated, and the real-time concentration of the reaction solution in the reaction chamber is obtained through a pre-established standard curve. After the linear array detection module 203 completes the detection of one reaction chamber, it continues to slide to the adjacent next reaction chamber and repeats the above detection process. At the same time, the micro spectrometer real-time monitors the spectral and light intensity changes of the illumination light source. When the threshold of the set power is exceeded, the control system 17 adjusts the duty cycle of the control signal to increase the light output intensity.

[0120] The linear array design in this embodiment can also integrate reaction and real-time detection, simplify the reactor structure to a certain extent, reduce the manufacturing cost, and the linear reciprocating detection method is also easy to control and maintain, improving the system stability.

[0121] In summary, the linear array photoreaction device 200 in this embodiment realizes automatic round-trip detection of multiple reaction chambers through the detection unit reciprocating on the linear track, and has the advantages of simple structure, easy control, lower cost, etc., thus providing another beneficial technical solution for high-throughput reaction and real-time monitoring of photoreaction.

[0122] Embodiment Seven

[0123] In some embodiments, the lamp group of the illumination light source can use an array composed of 6 LED lamp beads in series to provide illumination for each reaction well. Although this design can flexibly adjust the light intensity, there are still some inherent limitations. The light emitted by the LED array is divergent, resulting in significant scattering losses and uneven illumination of the reaction solution. Part of the light is absorbed by the reaction channel wall, reducing the overall energy efficiency of the photoreaction process. To overcome these defects, this embodiment proposes an improved light source configuration that uses a single high-power LED and is coupled with a collimating lens system. In this embodiment, each reaction well is illuminated by a separate LED light source, and the collimating lens assembly is integrated with the LED. The lens system is used to collect the divergent light emitted by the LED and reshape it into a collimated beam. After collimation, the light rays emitted from the lens assembly exhibit nearly parallel characteristics and can maintain a constant beam diameter when propagating through the reaction solution.

[0124] This collimated illumination method has several significant advantages compared to the divergent light of the previous bare LED array. First, the collimating lens focuses the light into a tightly parallel beam, ensuring that a larger proportion of photons pass through the reaction solution, reducing scattering and absorption losses, thereby improving the energy utilization efficiency and accelerating the reaction rate. Second, the collimated beam achieves a more uniform light intensity distribution throughout the reaction volume, which is crucial for maintaining consistent reaction conditions. Third, the highly directional nature of the collimated beam minimizes stray light and crosstalk between adjacent channels.

[0125] In summary, the improved single-LED light source integrated with a collimating lens in this embodiment reduces energy loss, improves reaction uniformity, and improves the efficiency and accuracy of the photoreactor by shaping the emitted light into a uniform and low-divergence beam.

[0126] Embodiment Eight

[0127] This embodiment is a further experimental illustration of the improved solution of the present invention.

[0128] As a preferred embodiment, the luminous power range of each lighting unit is 0 - 203 mw. The eight reaction chambers are annularly distributed in the reaction cavity 5. Then, the eight lighting units correspond to the eight reaction chambers one by one, and each lighting unit is located below the corresponding reaction chamber to provide illumination light source energy for the photoreactive substances in the reaction chamber. The temperature control and heat dissipation method adopts disk copper tube water cooling for temperature control.

[0129] The laser emitted by the laser assembly 6 - 3 is single - wavelength laser with a wavelength of 650 nm; the first signal detector 6 - 2 is a photodetector.

[0130] The influence of Escherichia coli (NST74) bacterial liquid with different dilution concentrations on the laser transmittance was measured using this photoreaction device, and the relationship between the bacterial liquid concentration and the voltage obtained by converting the light intensity after the laser passed through the reaction solution was established to establish an OD - lg(V0 / V t ) curve. The test data are as Figure 20 shown: Figure 20 (A) is a graph showing the relationship between OD measured by an enzyme - linked immunosorbent assay (ELISA) reader and the dilution factor at different dilution multiples; Figure 20 (B) is a graph showing the relationship between lg(V0 / V t ) measured by the photoreaction device and the dilution factor at different dilution multiples; Figure 20 (C) is Figure 20 (A) - Figure 20 (B) The linear fitting curve of the measured OD value and lg(V0 / V t ) in the figure.

[0131] The experimental results show that there is a significant linear relationship between the OD value and lg(V0 / V t ). The detection method adopted by the improved photoreaction device in the embodiment of the present invention can effectively reflect the change of the bacterial liquid concentration, and the OD value measured by the ELISA reader and lg(V0 / V t ) measured by the photoreaction device can establish a standard curve. Through statistical analysis, the correlation coefficient (R 2 = 0.998) shows a good linear fitting, further verifying the reliability of the multifunctional photoreaction device provided in this embodiment.

[0132] Example Nine

[0133] This embodiment is a further experimental illustration of the improved solution of the present invention.

[0134] The wavelengths of the lasers 6-3-1 turned on in the laser assembly 6-3 are 250 nm, 275 nm, 300 nm, 325 nm, 350 nm, 375 nm, 400 nm, 425 nm, 450 nm, 475 nm, 500 nm, 525 nm, and 550 nm. The first signal detector 6-2 is a micro spectrometer, and a Hamamatsu micro spectrometer, model C12880MA, is used.

[0135] The discrete absorption spectral data during the reduction of p-nitrophenol to p-aminophenol were measured using this photoreaction device. After adding silver / polymer hybrid nanoparticles to the mixture of p-nitrophenol and sodium borohydride, the mixture was stirred evenly, and the photoreaction device detected it every 2 min in the discrete wavelength range of 250 - 550 nm. The corresponding detection data are as Figure 21 shown: Figure 21 (A) shows the reactants and reaction products before and after the reaction; Figure 21 (B) shows the discrete absorption spectral diagram obtained by detection.

[0136] The experimental results show that after adding silver / polymer hybrid nanoparticles, the ultraviolet absorption peak of the nitro group at 400 nm gradually decreases with the progress of the reaction, and the ultraviolet absorption peak of the amino group at 300 nm gradually increases. Therefore, p-nitrophenol is reduced to p-aminophenol. The detection method used in the improved photoreaction device in the embodiment of the present invention can effectively detect the absorption spectral data during the reaction process and track the reaction progress.

[0137] It should be noted that the specific working processes of each unit provided in the above embodiments of the present application can be correspondingly referred to the corresponding steps in the foregoing operation method embodiments, and will not be elaborated here.

[0138] Those skilled in the art can further realize that the modules or units and method steps of each example described in combination with the embodiments disclosed herein can be implemented by electronic hardware, computer software, or a combination of the two. To clearly illustrate the interchangeability of hardware and software, the composition and steps of each example have been generally described according to functions in the above description. Whether these functions are executed in a hardware or software manner depends on the specific application and design constraints of the technical solution. Those skilled in the art can use different methods to implement the described functions for each specific application, but such implementation should not be considered to exceed the scope of the present application.

[0139] In summary, the present invention proposes a multifunctional photoreaction device and a corresponding usage method. By using an improved monitoring module, real-time detection and monitoring of state parameters are carried out during the photoreaction process through a rotation detection mechanism. And through the control module, the light source of the photoreaction is adjusted according to the detection results, and the working state of the photoreaction device such as the light source life detection is controlled and adjusted, so as to realize the integration of the whole process of the reaction and detection of the photoreactant, significantly reduce the complexity of manual operation and the pollution risk, and realize the real-time and accurate monitoring of the reaction curve and concentration of the photoreactant.

[0140] As described above, the above is only the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto. Any changes or substitutions that can be thought of by those skilled in the art within the technical scope disclosed by the present invention without creative labor should be covered within the protection scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope defined by the claims.

Claims

1. A multifunctional photoreaction device, characterized in that: The photoreaction device comprises: a containing chamber (101), a reaction chamber (102), a monitoring module (103), a light emitting module (104), and a control module (106); wherein the reaction chamber (102) is used for performing a photoreaction, the light emitting module (104) is used for providing a light source for the photoreaction, and the monitoring module (103) is used for performing real-time detection of a state signal during the photoreaction process; The accommodating chamber (101) is a structure that defines an internal space and accommodates internal modules, and the reaction chamber (102), the monitoring module (103), the light emitting module (104), and the control module (106) are integrated in the accommodating chamber (101); The reaction chamber (102) comprises at least one or more reaction chambers; the reaction chamber is used to place a photoreaction container; the light emitting module (104) is used to provide an illumination light source for the photoreaction; The monitoring module (103) comprises at least a first signal detector (6-2); the first signal detector (6-2) is used to detect the optical signal parameters transmitted from the reaction solution in the optical reaction container; The control module (106) is used to communicate and control and adjust each module in the light reaction device according to the state signal detected by the monitoring module (103).

2. A multifunctional photoreaction device according to claim 1, characterized in that: The monitoring module (103) further comprises a second signal detector (6-5), wherein the second signal detector (6-5) is used to detect light signal parameters of the illumination light source.

3. A multifunctional photoreaction device according to claim 1 or 2, characterized in that: Each reaction chamber is composed of a reaction tube (2) and a reaction tube jacket (3); the outer diameters of the plurality of reaction tube jackets (3) are the same, and the inner diameters are the same or different; the reaction tube jackets (3) are used to fix the reaction tubes (2) of corresponding inner diameters and place them in the reaction chamber.

4. A multifunctional photoreaction device according to claim 1 or 2, characterized in that: The light emitting module (104) comprises one or more light emitting units arranged around a plurality of reaction chambers, wherein the light emitting units are light emitting components corresponding to the reaction chambers one by one; each light emitting unit is independently or non-independently regulated, independently or non-independently disassembled and installed.

5. A multifunctional photoreaction device according to claim 1 or 2, characterized in that: The monitoring module (103) at least comprises a light detector (6), wherein the light detector (6) comprises a motor housing (6-1), a first signal detector (6-2), a laser assembly (6-3), a concave rocker arm (6-4), a motor bottom cover (6-6), a motor seat (6-7), a detection motor (6-8), a motor connecting shaft (6-9), a PCB disc-type conductive slip ring (6-10), and a rocker arm connecting shaft (6-11); The PCB disc-type conductive slip ring (6-10) is composed of a slip ring stator and a slip ring rotor; the detection motor (6-8) is fixed in the central through hole of the reaction chamber (5) after being encapsulated by a motor housing (6-1), a motor seat (6-7) and a motor bottom cover (6-6); the rocker arm connecting shaft (6-11) is connected to the motor connecting shaft (6-9) inside the detection motor (6-8), and the other end of the rocker arm connecting shaft (6-11) is connected to the concave rocker arm (6-4), so that the rotation of the detection motor (6-8) drives the concave rocker arm (6-4) to rotate; The first signal detector (6-2) and the laser assembly (6-3) are respectively arranged in the slots at both ends of the concave rocker arm (6-4); the slip ring stator of the PCB disc-type conductive slip ring (6-10) is fixed at the upper end of the motor base (6-7); and the slip ring rotor is fixed at the cover of the rocker arm connecting shaft (6-11).

6. A multifunctional photoreaction device according to claim 5, characterized in that: After the detection motor 6-8 is powered on, the detection motor (6-8) drives the detection assembly including the laser assembly (6-3), the first signal detector (6-2), and the concave rocker arm (6-4) to perform precise intermittent rotation. When the detection assembly rotates to the center of the reaction tube (2), the detection motor (6-8) stops rotating. At this time, the laser emitted by the laser assembly (6-3) passes through the reaction tube (2), and the first signal detector (6-2) receives the spectral signal transmitted from the reaction tube (2) and performs spectral signal detection. After completing the detection, the detection motor (6-8) continues to rotate to the next reaction tube (2) and performs the same detection on the reaction tube (2).

7. A multifunctional photoreaction device according to claim 5 or 6, characterized in that: The optical detector (6) further comprises a second signal detector (6-5) which is fixed at the center of the bottom rod of the concave rocker arm (6-4). When the second signal detector (6-5) is located at the illumination light source, the optical signal of the illumination light source is detected.

8. The multifunctional photoreaction device according to claim 2, characterized in that: The first signal detector (6-2) is a photoelectric detector or a micro-spectrometer; the second signal detector (6-5) is a photoelectric detector or a micro-spectrometer.

9. A method for using the multifunctional photoreaction device according to any one of claims 1 to 8, characterized in that: The method comprises: Determining the size and number of photoreaction containers in the reaction chamber (102) according to the photoreaction to be performed; Setting the number of lighting units turned on and the light intensity required for the light reaction in the light-emitting module (104), and selecting a lighting light source with a corresponding wavelength according to different reaction media; Starting the light reaction device to perform light reaction; The monitoring module (103) performs real-time detection of the state signal during the light reaction process, and the control module (106) regulates each module in the light reaction device according to the state signal detection result of the monitoring module (103).

10. The method for using the multifunctional photoreaction device according to claim 9, characterized in that: The monitoring module (103) performs real-time detection of the state signal during the light reaction process, and the control module (106) regulates each module in the light reaction device according to the state signal detection result of the monitoring module (103), specifically including: The detection motor (6-8) in the monitoring module (103) drives the entire detection system including the laser assembly (6-3) and the first signal detector to intermittently rotate at a preset angle; each time the rotation stops, the laser emitted by the laser assembly (6-3) passes through the reaction tube (2) in the reaction chamber (102), and the first signal detector (6-2) collects the light signal transmitted from the reaction tube (2), calculates the light absorption characteristics of the reactants in the reaction tube (2) by analyzing the light signal, and monitors the concentration change and / or spectral dynamic characteristics of the reactants in real time; At the same time, the control module (106) at least adjusts the brightness of the illumination light source in real time according to the detected state signal, so as to ensure that the photoreactive substance reacts under the optimal growth conditions.

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