Dendrobium officinale oral liquid as well as preparation method and application thereof

Abstract

The invention relates to the technical field of preparation of traditional Chinese medicine preparations, and particularly discloses dendrobium officinale oral liquid and a preparation method and application thereof.The preparation method includes the steps that dendrobium officinale raw materials are cleaned and crushed to 0.5-1.5 mm, a citric acid buffer solution with the specific pH value is added, the materials are mixed according to the mass ratio of 8-12: 1, and the materials are soaked at the temperature of 40-50 DEG C; then adding a compound enzyme preparation which contains cellulase, pectinase and xylanase and has a specific enzyme activity ratio, the total addition amount of the compound enzyme preparation is 1.5-2.5% of the dry weight of the raw materials, and carrying out enzymolysis at 50-60 DEG C; after inactivating the enzymatic hydrolysate, adding a 60-70% ethanol water solution, carrying out ultrasonic-assisted extraction, centrifuging the extracting solution, repeatedly extracting and concentrating; and adding high-purity sorbitol, beta-cyclodextrin with a specific substitution degree and L-sodium ascorbate into the concentrated solution according to a mass ratio, adjusting the pH value, filtering the mixed solution with a 0.22 mu m microporous filter membrane, carrying out segmented heating sterilization at 121 DEG C, cooling to 25-30 DEG C, filling nitrogen, and sealing. According to the method, effective components can be efficiently extracted, the product quality is guaranteed, and the prepared oral liquid is high in quality, good in taste and high in safety.

Description

Technical Field

[0001] The present invention relates to the technical field of traditional Chinese medicine preparation, and specifically to an oral liquid of Dendrobium officinale, its preparation method and application. Background Art

[0002] Dendrobium officinale Kimura et Migo is a perennial herb of the genus Dendrobium in the Orchidaceae family. It is rich in various bioactive components such as polysaccharides, alkaloids, flavonoids, phenols, and amino acids, and shows significant effects in immune regulation, antioxidant, hypoglycemic, hypolipidemic, anti-tumor, liver and kidney protection, etc. With the improvement of people's health awareness and the increasing attention to health care, the market demand for Dendrobium officinale-related products continues to grow. Among them, the oral liquid of Dendrobium officinale is favored by consumers due to its advantages such as convenient administration and rapid absorption.

[0003] At present, many studies and patents on the preparation method of the oral liquid of Dendrobium officinale have been published. For example, Patent CN201611031823.6 discloses an oral liquid of Dendrobium officinale and its preparation method. Each liter of the oral liquid is prepared from 10 - 50 g of Dendrobium officinale (fresh stems), 2 - 10 g of chrysanthemum, 2 - 12 g of cinnamon, 0.2 - 0.7 g of sodium alginate, 0.1 - 0.5 g of citric acid, 0.05 - 0.3 g of sodium citrate, 0.15 - 0.45 g of aspartame and an appropriate amount of drinking water. This method separates the juice of fresh Dendrobium officinale stems, decocts chrysanthemum and cinnamon, dissolves sodium alginate and potassium sorbate, and then formulates after dissolving other components. However, this method has some limitations. For example, the processing method of raw materials is relatively simple, the extraction rate of effective components in Dendrobium officinale may not be high, and sweeteners such as aspartame added in the formula may not be suitable for some special populations.

[0004] Another example is that Patent CN201610089805.7 discloses an oral liquid of Dendrobium officinale with the function of enhancing immunity and its production method, including Dendrobium officinale, red ginseng, Ophiopogon japonicus, Schisandra chinensis, potassium sorbate, xylitol, acesulfame potassium, and citric acid. Among them, Dendrobium officinale accounts for 20 - 30% of the total mass of the materials, red ginseng accounts for 10 - 30% of the total mass of the materials, Ophiopogon japonicus accounts for 10 - 30% of the total mass of the materials, Schisandra chinensis accounts for 10 - 30% of the total mass of the materials, potassium sorbate accounts for 0.01 - 0.02% of the total mass of the materials, xylitol accounts for 5 - 10% of the total mass of the materials, acesulfame potassium accounts for 0.01 - 0.03% of the total mass of the materials, and citric acid accounts for 1 - 3% of the total mass of the materials. The process adopted by this method is clean and hygienic, and the extraction rate of medicinal properties is high. However, there are many drug components in the formula, the cost is relatively high, and the synergistic effect between different drugs may be relatively complex, affecting the stability of product quality.

[0005] For another example, Patent CN201610136762.3 discloses a dendrobium officinale oral liquid, which comprises raw materials in the following weights: 28 kg of dendrobium officinale, 14 kg of ophiopogon japonicus, 14 kg of american ginseng, 28 kg of polygonatum sibiricum, and 14 kg of wolfberry fruit. However, this patent application was ultimately rejected, and its preparation method may have problems in terms of process feasibility, product quality stability, etc.

[0006] A preparation method of a compound whole turtle, soft-shelled turtle and dendrobium officinale nourishing yin oral liquid disclosed in Patent CN200410025480.3 is composed of medicinal materials such as whole turtle, soft-shelled turtle, dendrobium officinale, american ginseng, ophiopogon japonicus, polygonatum odoratum, xylitol, etc. In this method, the whole turtle and soft-shelled turtle are first washed and decocted three times, and the juice is reserved for later use; then the four traditional Chinese medicines of american ginseng, dendrobium officinale, ophiopogon japonicus, and polygonatum odoratum are decocted twice, the residue is removed and filtered, after concentration, it is cooled, ethanol is added, stirred evenly and left for 24 hours. After recovering the ethanol, xylitol is added and stirred evenly. The filtrate is concentrated to an extract state, diluted with water, filtered, and then an appropriate amount of distilled water is added; refrigerated, filtered, sealed in a 10-ml bottle and sealed; sterilized with boiling water to obtain. The oral liquid prepared by this method has functions such as nourishing yin and strengthening yang, and tonifying both qi and blood, but the preparation process is relatively cumbersome, involving multiple decoctions and complex steps such as concentration and alcohol precipitation, and the production efficiency is relatively low.

[0007] Patent CN202010318129.2 discloses a dendrobium officinale stomach-protecting oral liquid and its preparation method. The raw materials used include dendrobium officinale, notoginseng, bletilla striata, Aucklandia lappa, and purified water. This oral liquid is easily absorbed by the human body and can improve gastrointestinal function, but there is still room for improvement in the extraction of active ingredients and the stability of the product.

[0008] In summary, the existing preparation methods of dendrobium officinale oral liquid have deficiencies to varying degrees in terms of the extraction rate of active ingredients, product stability, taste, cost, and applicable population. In order to prepare a dendrobium officinale oral liquid with excellent quality, remarkable efficacy, high safety, and a wide range of applicable population, it is necessary to further optimize the preparation process, screen suitable raw materials and additives to improve product quality and market competitiveness. Therefore, developing a new preparation method for dendrobium officinale oral liquid has important practical significance and market value. Summary of the Invention

[0009] (1) Technical problems to be solved

[0010] In view of the deficiencies of the prior art, the present invention provides a dendrobium officinale oral liquid, its preparation method and application, and solves the problems raised in the above background technology.

[0011] (2) Technical solutions

[0012] In order to achieve the above object, the present invention discloses a preparation method of a pancreatic lipase inhibitor, which comprises the following steps:

[0013] Step 1: Wash the raw Dendrobium officinale materials and crush them to a particle size of 0.5 - 1.5 mm. Add a citric acid buffer solution with a pH value of 5.0 - 6.5 and soak them at 40 - 50°C for 30 - 60 min.

[0014] Step 2: Add a complex enzyme preparation to the soaked materials. The complex enzyme preparation contains cellulase, pectinase, and xylanase, and the enzyme activity ratio is 1:0.8 - 1.2:0.5 - 0.8. Enzymatically hydrolyze at 50 - 60°C for 2 - 4 h.

[0015] Step 3: Heat the enzymatic hydrolysate to 80 - 90°C to inactivate the enzyme activity. Then add an ethanol aqueous solution with an ethanol volume concentration of 60 - 70%, and a solid - liquid mass ratio of 1:15 - 20. Assist extraction with ultrasound for 30 - 50 min at a frequency of 40 - 60 kHz.

[0016] Step 4: Centrifuge and filter the extract. Repeat the extraction in Step 3 once for the filter residue, combine the two filtrates, and concentrate under reduced pressure at 60 - 70°C and a vacuum of 0.08 - 0.1 MPa to a relative density of 1.10 - 1.20.

[0017] Step 5: Add sorbitol, β - cyclodextrin, and sodium L - ascorbate to the concentrated solution, mix evenly, and adjust the pH to 4.5 - 5.5. The mass ratio of the concentrated solution, sorbitol, β - cyclodextrin, and sodium L - ascorbate is 100:5 - 8:2 - 4:0.1 - 0.3.

[0018] Step 6: Filter the mixed solution through a 0.22 - μm microporous membrane, and then sterilize it at 121°C for 15 - 20 min.

[0019] Step 7: Cool the sterilized liquid to 25 - 30°C, fill it into sterile glass bottles, and seal it with nitrogen.

[0020] Preferably, the mass ratio of the citric acid buffer solution to the raw Dendrobium officinale materials in Step 1 is 8 - 12:1.

[0021] Preferably, the total addition amount of the complex enzyme preparation in Step 2 is 1.5 - 2.5% of the dry weight of the raw Dendrobium officinale materials.

[0022] Preferably, the ultrasonic power in Step 3 is 300 - 500 W, and the extraction temperature is 50 - 60°C.

[0023] Preferably, the centrifugal speed in Step 4 is 8000 - 10000 rpm, and the centrifugal time is 10 - 15 min.

[0024] Preferably, the sorbitol in Step 5 is crystalline, with a purity ≥98%, and the degree of substitution of β - cyclodextrin is 0.6 - 0.8.

[0025] Preferably, in the sterilization process of step six, the temperature is increased in stages. In the first stage, the temperature is increased to 80°C at a rate of 2°C / min and held for 5 minutes. In the second stage, the temperature is increased to 121°C at a rate of 3°C / min.

[0026] Preferably, in step seven, the nitrogen filling pressure is 0.2 - 0.3 MPa, and the oxygen content in the bottle after sealing is ≤ 0.5%.

[0027] Another object of the present invention is to provide a Dendrobium officinale oral liquid, which comprises the following components in mass ratio: 100 parts of Dendrobium officinale extraction concentrate, 5 - 8 parts of sorbitol, 2 - 4 parts of β-cyclodextrin, 0.1 - 0.3 parts of L-sodium ascorbate, and the pH value of the oral liquid is 4.5 - 5.5. Among them, the degree of substitution of β-cyclodextrin is 0.6 - 0.8, sorbitol is crystalline and the purity is ≥ 98%, and the oxygen content in the bottle after the oral liquid is filled with nitrogen and sealed is ≤ 0.5%.

[0028] Another object of the present invention is to provide an application of the Dendrobium officinale oral liquid for preparing a food composition with the function of regulating intestinal flora.

[0029] (III) Beneficial technical effects

[0030] I. Efficient extraction of active ingredients

[0031] In the raw material pretreatment stage, the Dendrobium officinale raw materials are washed and then crushed to a particle size of 0.5 - 1.5 mm, and a citric acid buffer solution with a specific pH value (5.0 - 6.5) is added, and soaked at 40 - 50°C for 30 - 60 minutes. This fine treatment method can initially destroy the plant cell wall structure, making the active ingredients in the cells more easily dissolved, laying a good foundation for subsequent extraction. Compared with some traditional methods, such as simple direct decoction or extraction after rough crushing, the initial dissolution rate of active ingredients can be significantly increased, and it is initially estimated that it can be increased by about 15% - 20%. Then a composite enzyme preparation containing cellulase, pectinase and xylanase with an enzyme activity ratio of 1:0.8 - 1.2:0.5 - 0.8 is used, and enzymolysis is carried out at 50 - 60°C for 2 - 4 hours. This composite enzyme preparation acts precisely on the main components of the Dendrobium officinale cell wall, further decomposes the cell wall, and releases more encapsulated polysaccharides, alkaloids and other active ingredients. Compared with the process without using the composite enzyme or with an unreasonable enzyme activity ratio, the extraction rate of active ingredients can be additionally increased by 20% - 30%.

[0032] In the subsequent ultrasonic-assisted extraction step, after adding an ethanol aqueous solution (ethanol volume concentration of 60-70%, solid-liquid mass ratio of 1:15-20), ultrasound is applied at a frequency of 40-60kHz for 30-50min, while an extraction temperature of 50-60°C and an ultrasonic power of 300-500W are used. The ultrasonic cavitation effect, mechanical vibration and other effects can be utilized to accelerate the diffusion of effective ingredients from the raw materials to the solvent, thereby increasing the overall effective ingredient extraction rate by 30%-40% compared to the conventional single thermal extraction method, thereby retaining the medicinal value of Dendrobium officinale to the greatest extent.

[0033] 2. Product quality is stable and reliable

[0034] During the preparation process, the process parameters of each step are strictly controlled. For example, in the inactivation step after enzymolysis, the enzymolysis solution is heated to 80-90°C to accurately inactivate the enzyme activity to avoid the residual enzyme from continuing to affect the product quality. In the concentration step, the relative density is 1.10-1.20 at 60-70°C and a vacuum degree of 0.08-0.1MPa, which not only ensures that the active ingredients are not destroyed by high temperature, but also accurately controls the degree of concentration to ensure the consistency of the concentration of each batch of products. The selection and use of additives are also very sophisticated. Sorbitol (crystalline, purity ≥98%), β-cyclodextrin (degree of substitution is 0.6-0.8) and L-sodium ascorbate are added to the concentrate, and the mass ratio of the concentrate, sorbitol, β-cyclodextrin, and L-sodium ascorbate is 100:5-8:2-4:0.1-0.3 for mixing. As a high-quality sweetener, sorbitol not only provides a suitable taste, but also has high stability and is not prone to moisture absorption and deterioration; β-cyclodextrin can encapsulate some unstable or volatile active ingredients to improve their stability and prevent the loss of effective ingredients during storage; sodium L-ascorbate has an antioxidant effect and can effectively delay the oxidation and deterioration of the product, thereby extending the shelf life of the product by 3-6 months at room temperature.

[0035] 3. Excellent taste and wide application

[0036] High-purity crystalline sorbitol is used as a sweetener. Compared with traditional sucrose and aspartame, it has appropriate sweetness, a refreshing and pure taste, no peculiar smell or bad aftertaste, and can meet the taste needs of most consumers, especially for diabetics, obese people and other groups with special requirements for sugar intake. By rationally adjusting the proportion of additives, the taste of the product is optimized, making it smooth in the mouth, without obvious stickiness or granularity, and providing a good taking experience, which improves consumer compliance and expands the range of applicable populations of the product.

[0037] 4. Safety, hygiene and compliance with standards

[0038] The entire preparation process strictly adheres to food and drug production specifications. Starting from the cleaning of raw materials, it ensures the removal of harmful substances such as surface impurities and pesticide residues. During the material transfer and processing in each link, a sterile operating environment is adopted to reduce the risk of microbial contamination. The mixed solution is filtered through a 0.22μm microporous membrane, which can effectively intercept impurities such as bacteria and particles, ensuring the purity of the product. Subsequently, it is sterilized at 121°C for 15 - 20 minutes, and a segmented temperature increase is adopted (the first stage is heated to 80°C at a rate of 2°C / min and held for 5 minutes, the second stage is heated to 121°C at a rate of 3°C / min), which not only ensures the sterilization effect but also reduces the impact of high temperature on the product quality, ensuring that the product meets strict hygiene and safety standards and making consumers more at ease when taking it. Detailed implementation mode

[0039] To facilitate the understanding of the present invention, the present invention will be described more comprehensively below. The preferred embodiments of the present invention are given below. However, the present invention can be implemented in many different forms and is not limited to the embodiments described herein. On the contrary, the purpose of providing these embodiments is to make the understanding of the disclosure content of the present invention more thorough and comprehensive.

[0040] Example 1

[0041] A preparation method of Dendrobium officinale oral liquid includes the following steps:

[0042] Step 1: After cleaning the Dendrobium officinale raw materials, they are crushed to a particle size of 0.8 mm, and a citric acid buffer solution with a pH value of 5.5 (the mass ratio of the buffer solution to the raw materials is 10:1) is added, and soaked at 45°C for 45 minutes.

[0043] Step 2: A compound enzyme preparation (the enzyme activity ratio of cellulase, pectinase, and xylanase is 1:1.0:0.6) is added to the soaked raw materials, and the total addition amount is 2.0% of the dry weight of the raw materials, and enzymolysis is carried out at 55°C for 3 hours.

[0044] Step 3: The enzymolysis solution is heated to 85°C to inactivate the enzyme, and an ethanol aqueous solution with a volume concentration of 65% (the solid-liquid mass ratio is 1:18) is added, and assisted extraction is carried out at an ultrasonic power of 400 W, a frequency of 50 kHz, and a temperature of 55°C for 40 minutes.

[0045] Step 4: The extract is centrifuged at 9000 rpm for 12 minutes, the filter residue is extracted again, the filtrates are combined, and concentrated under reduced pressure to a relative density of 1.15 at 65°C and a vacuum degree of 0.09 MPa.

[0046] Step 5: Sorbitol (purity ≥ 98%), β-cyclodextrin (substitution degree 0.7), and sodium L-ascorbate (mass ratio 100:6:3:0.2) are added to the concentrated solution, and after mixing evenly, the pH is adjusted to 5.0.

[0047] Step 6: After filtering the mixed solution through a 0.22-μm filter membrane, perform stepwise heating sterilization: In the first stage, heat it at 2°C / min to 80°C and hold for 5 min. In the second stage, heat it at 3°C / min to 121°C and sterilize for 18 min.

[0048] Step 7: Cool the sterilized solution to 28°C, fill it into a sterile glass bottle, apply a nitrogen filling pressure of 0.25 MPa, and after sealing, the oxygen content in the bottle is ≤0.5%.

[0049] Example 2

[0050] A preparation method of Dendrobium officinale oral liquid, comprising the following steps:

[0051] Step 1: Crush the Dendrobium officinale raw material to a particle size of 1.2 mm, add a citric acid buffer solution with a pH value of 6.0 (the mass ratio of the buffer solution to the raw material is 12:1), and soak at 50°C for 30 min.

[0052] Step 2: The addition amount of the compound enzyme preparation (enzyme activity ratio 1:1.2:0.5) is 2.5% of the dry weight of the raw material, and enzymolysis is carried out at 60°C for 2.5 h.

[0053] Step 3: Heat the enzymolysis solution to 90°C to inactivate the enzyme, add ethanol with a volume concentration of 70% (solid-liquid ratio 1:20), and extract at an ultrasonic power of 500 W, a frequency of 60 kHz, and a temperature of 60°C for 30 min.

[0054] Step 4: Centrifuge the extract at 10000 rpm for 10 min, repeat the extraction of the filter residue, and after combining the filtrates, concentrate to a relative density of 1.20 at 70°C and a vacuum degree of 0.1 MPa.

[0055] Step 5: Add sorbitol, β-cyclodextrin (substitution degree 0.8), sodium L-ascorbate (mass ratio 100:8:4:0.3), and adjust the pH to 5.5.

[0056] Step 6: Heat stepwise to 121°C and sterilize for 15 min.

[0057] Step 7: Cool to 25°C, apply a nitrogen filling pressure of 0.3 MPa, and after sealing, the oxygen content is ≤0.5%.

[0058] Comparative Example 1

[0059] A preparation method of Dendrobium officinale oral liquid, which is different from Example 1 in that:

[0060] Step 2: Only use cellulase (pectinase and xylanase are not added), and the addition amount is 2.0% of the dry weight of the raw material.

[0061] Step 3: Instead of using ethanol-assisted extraction, change to pure water extraction.

[0062] Comparative Example 2

[0063] A preparation method of Dendrobium officinale oral liquid, which is different from Example 1 in that:

[0064] Step five: Without adding β-cyclodextrin and sodium L-ascorbate, only adding sorbitol (mass ratio 100:6).

[0065] Step six: Directly heating to 121 °C without staged control.

[0066] Performance test:

[0067] Perform performance tests on the oral liquids prepared in Examples 1-2 and Comparative Examples 1-2, and the results are as follows:

[0068] Table 1 Extraction rate and stability of active ingredients

[0069]

[0070] Table 2 Bacteriostatic effect and sensory evaluation

[0071]

[0072] Result analysis:

[0073] In Examples 1-2, due to the adoption of compound enzymatic hydrolysis and ethanol ultrasonic extraction, the extraction rates of polysaccharides and flavonoids are significantly higher than those in Comparative Example 1 (single enzymatic hydrolysis) and Comparative Example 2 (without adding embedding agent).

[0074] The addition of β-cyclodextrin and sodium L-ascorbate (Examples 1-2) effectively improves the stability of the oral liquid, and the precipitation rate is lower than that in Comparative Example 2.

[0075] The staged sterilization and nitrogen filling process (Examples 1-2) ensures the bacteriostatic rate and sensory quality of the product, while in Comparative Example 2, direct high-temperature sterilization leads to the degradation of some active ingredients and a decrease in the taste score.

[0076] Example 3

[0077] A preparation method of Dendrobium officinale oral liquid includes the following steps:

[0078] Step one: Wash the Dendrobium officinale raw materials and crush them to a particle size of 1.0 mm, add a citric acid buffer solution with a pH value of 5.8 (the mass ratio of the buffer solution to the raw materials is 9:1), and soak at 48 °C for 50 min.

[0079] Step two: Add a compound enzyme preparation (the enzyme activity ratio of cellulase, pectinase, and xylanase is 1:0.9:0.7) to the soaked raw materials, with a total addition amount of 1.8% of the dry weight of the raw materials, and carry out enzymatic hydrolysis at 58 °C for 3.5 h.

[0080] Step 3: Heat the enzymolysis solution to 88°C to inactivate the enzymes, add an ethanol aqueous solution with a volume concentration of 62% (solid-liquid mass ratio 1:16), and perform assisted extraction for 45 min under the conditions of an ultrasonic power of 450 W, a frequency of 55 kHz, and a temperature of 58°C.

[0081] Step 4: Centrifuge the extract at 8500 rpm for 14 min, repeat the extraction for the filter residue once, and after combining the filtrates, concentrate under reduced pressure to a relative density of 1.18 at 62°C and a vacuum degree of 0.085 MPa.

[0082] Step 5: Add sorbitol (purity ≥ 98%), β-cyclodextrin (degree of substitution 0.65), and sodium L-ascorbate (mass ratio 100:5.5:2.5:0.15) to the concentrated solution, mix evenly, and then adjust the pH to 4.8.

[0083] Step 6: After filtering the mixed solution through a 0.22-μm filter membrane, perform segmented heating sterilization: in the first stage, heat to 80°C at a rate of 2°C / min and hold for 5 min, and in the second stage, heat to 121°C at a rate of 3°C / min and sterilize for 17 min.

[0084] Step 7: Cool the sterilized solution to 27°C, fill it into sterile glass bottles, charge nitrogen at a pressure of 0.22 MPa, and after sealing, the oxygen content in the bottle is ≤ 0.5%.

[0085] Comparative Example 3

[0086] A preparation method of Dendrobium officinale oral liquid, which is different from Example 3 in that:

[0087] In Step 2, a composite enzyme preparation was not used, and it was changed to a single pectinase (addition amount 2.0%), and the enzymolysis temperature was increased to 65°C.

[0088] In Step 5, sodium L-ascorbate was not added, and the mass ratio of sorbitol to β-cyclodextrin was adjusted to 100:6:3.

[0089] Comparative Example 4

[0090] A preparation method of Dendrobium officinale oral liquid, which is different from Example 3 in that:

[0091] In Step 3, the ethanol volume concentration was reduced to 50%, and the ultrasonic power was adjusted to 200 W.

[0092] In Step 6, the segmented heating was cancelled, and it was directly heated to 121°C at a rate of 5°C / min for sterilization for 20 min.

[0093] Performance test:

[0094] Perform performance tests on the oral liquids prepared in Example 3 and Comparative Examples 3-4, and the results are as follows:

[0095] Table 3 Comparison of active ingredients and antioxidant properties

[0096]

[0097] Table 4 Process Stability and Enzyme Inhibiting Activity

[0098]

[0099] Result Analysis:

[0100] In Example 3, due to the adoption of compound enzymolysis and optimized ethanol concentration, the extraction rates of polysaccharides and flavonoids are significantly higher than those in Comparative Example 3 (single enzymolysis) and Comparative Example 4 (low-concentration ethanol), and the DPPH free radical scavenging rate increases by more than 20%.

[0101] The synergistic effect of β-cyclodextrin and sodium L-ascorbate (Example 3) effectively inhibits the color change during storage (ΔE is only 1.2), while in Comparative Example 3, due to the absence of antioxidant, ΔE reaches 5.7.

[0102] The fractional sterilization process (Example 3) controls the polysaccharide loss rate within 3.1%, while direct high-temperature sterilization in Comparative Example 4 leads to an increase in the loss rate to 8.4%.

[0103] Example 4

[0104] A preparation method of Dendrobium officinale oral liquid, comprising the following steps:

[0105] Step 1: Crush the raw materials to a particle size of 0.5 mm, add a citric acid buffer solution with a pH value of 5.0 (mass ratio 8:1), and soak at 40°C for 60 min.

[0106] Step 2: Add 1.5% of a compound enzyme preparation (enzyme activity ratio 1:0.8:0.5) and enzymolyze at 50°C for 4 h.

[0107] Step 3: Use ethanol with a volume concentration of 60% (solid-liquid ratio 1:15), ultrasonic power of 300 W, frequency of 40 kHz, and extract at 50°C for 50 min.

[0108] Step 4: Centrifuge at 8000 rpm for 15 min, and concentrate the filtrate at 60°C and a vacuum degree of 0.08 MPa to a relative density of 1.10.

[0109] Step 5: Add sorbitol, β-cyclodextrin (substitution degree 0.6), and sodium L-ascorbate (mass ratio 100:5:2:0.1), and adjust the pH to 4.5.

[0110] Step 6: Sterilize by stepwise heating, with a total time of 20 min.

[0111] Step 7: Fill with nitrogen at a pressure of 0.2 MPa, and the oxygen content ≤ 0.5%.

[0112] Comparative Example 5

[0113] A preparation method of Dendrobium officinale oral liquid, different from Example 4 in that:

[0114] In Step 1, the soaking in citric acid buffer solution is cancelled and changed to soaking in pure water.

[0115] In Step 5, the degree of substitution of β-cyclodextrin is increased to 1.0, and the addition amount of sorbitol is increased to 10%.

[0116] Test description:

[0117] By comparing Example 4 with Comparative Example 5, it is found that:

[0118] Not using the citric acid buffer solution (Comparative Example 5) results in a 18.7% decrease in the polysaccharide extraction rate and a 25.4% decrease in the flavonoid content.

[0119] Excessive sorbitol and high-degree-of-substitution β-cyclodextrin (Comparative Example 5) increase the viscosity of the oral liquid to 35.2 mPa·s, and the taste score drops to 6.8 points (8.9 points for Example 4).

[0120] The above embodiments are only for illustrative purposes. Any parameter adjustment, process substitution, etc. within the scope of the claims of the present invention belong to the protection scope of the present invention.

[0121] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirits of the present invention. Any equivalent changes and improvements made within the scope of the application of the present invention should still fall within the scope covered by the patent of the present invention.

Claims

1. A preparation method of Dendrobium officinale oral liquid, characterized in that, It includes the following steps: Step 1: Wash the raw Dendrobium officinale materials and crush them to a particle size of 0.5 - 1.5 mm, add a citric acid buffer solution with a pH value of 5.0 - 6.5, and soak at 40 - 50 °C for 30 - 60 min; Step 2: Add a complex enzyme preparation to the soaked materials. The complex enzyme preparation contains cellulase, pectinase, and xylanase, and the enzyme activity ratio is 1:0.8 - 1.2:0.5 - 0.

8. Enzymatic hydrolysis is carried out at 50 - 60 °C for 2 - 4 h; Step 3: Heat the enzymatic hydrolysate to 80 - 90 °C. After inactivating the enzyme activity, add an ethanol aqueous solution with an ethanol volume concentration of 60 - 70%, a solid-liquid mass ratio of 1:15 - 20, and perform ultrasonic-assisted extraction for 30 - 50 min at a frequency of 40 - 60 kHz; Step 4: Centrifuge and filter the extract. Repeat the extraction in Step 3 once for the filter residue, combine the two filtrates, and concentrate under reduced pressure at 60 - 70 °C and a vacuum of 0.08 - 0.1 MPa to a relative density of 1.10 - 1.20; Step 5: Add sorbitol, β-cyclodextrin, and sodium L-ascorbate to the concentrated solution, mix evenly, and adjust the pH to 4.5 - 5.

5. The mass ratio of the concentrated solution, sorbitol, β-cyclodextrin, and sodium L-ascorbate is 100:5 - 8:2 - 4:0.1 - 0.3; Step 6: Filter the mixed solution through a 0.22 μm microporous membrane, and then perform high-temperature sterilization at 121 °C for 15 - 20 min; Step 7: Cool the sterilized liquid to 25 - 30 °C, fill it into a sterile glass bottle, and fill with nitrogen and seal.

2. The preparation method of a dendrobium officinale oral liquid according to claim 1, wherein, In Step 1, the mass ratio of the citric acid buffer solution to the raw Dendrobium officinale materials is 8 - 12:

1.

3. The preparation method of a dendrobium officinale oral liquid according to claim 1, characterized in that, In Step 2, the total addition amount of the complex enzyme preparation is 1.5 - 2.5% of the dry weight of the raw Dendrobium officinale materials.

4. The preparation method of a dendrobium officinale oral liquid according to claim 1, characterized in that, In Step 3, the ultrasonic power is 300 - 500 W, and the extraction temperature is 50 - 60 °C.

5. The preparation method of a dendrobium officinale oral liquid according to claim 1, wherein, In Step 4, the centrifugation speed is 8000 - 10000 rpm, and the centrifugation time is 10 - 15 min.

6. The preparation method of a dendrobium officinale oral liquid according to claim 1, wherein In Step 5, the sorbitol is crystalline, with a purity ≥ 98%, and the degree of substitution of β-cyclodextrin is 0.6 - 0.

8.

7. The preparation method of a dendrobium officinale oral liquid according to claim 1, characterized in that, In Step 6, the sterilization process uses stepwise heating. The first stage is heated to 80 °C at 2 °C / min and held for 5 min, and the second stage is heated to 121 °C at 3 °C / min.

8. The preparation method of a dendrobium officinale oral liquid according to claim 1, characterized in that, In Step 7, the nitrogen filling pressure is 0.2 - 0.3 MPa, and the oxygen content in the bottle after sealing is ≤ 0.5%.

9. A dendrobium officinale oral liquid, characterized in that, Prepared by the method according to any one of claims 1 - 8, it contains the following components in mass ratio: 100 parts of Dendrobium officinale extraction concentrate, 5 - 8 parts of sorbitol, 2 - 4 parts of β-cyclodextrin, 0.1 - 0.3 parts of sodium L-ascorbate, and the pH value of the oral liquid is 4.5 - 5.5, where the degree of substitution of β-cyclodextrin is 0.6 - 0.8, the sorbitol is crystalline and the purity ≥ 98%, and the oxygen content in the bottle after the oral liquid is filled with nitrogen and sealed is ≤ 0.5%.

10. Use of the dendrobium officinale oral liquid according to claim 9, characterized in that, It is used to prepare a food composition with the function of regulating intestinal flora.

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