Stem cell collecting and extracting device

Abstract

The invention discloses a stem cell collection and extraction device, belongs to the technical field of cell separation and extraction, and aims to solve the problems of complex and dispersed operation procedures, need of cooperation of a plurality of workers, investment of a large amount of manpower cost, low time efficiency, and high time efficiency caused by repeated secondary separation of a precipitation solution. In order to solve the problem that stem cells are mechanically damaged to a certain extent in the prior art, the stem cell extraction device comprises a working case and a discharging mechanism, the discharging mechanism is used for efficient extraction and arranged on the periphery of the bottom of a matching mechanism, and through the discharging mechanism, the continuous action of multiple sets of cyclone separation structures can be achieved; stem cells can be separated from other substances more effectively, a plurality of groups of cyclone separation structures can perform multiple times of fractional separation on liquid in the centrifugal process, and each stage of cyclone separator can perform primary separation and enrichment on components in the liquid, so that the stem cells can reach higher purity within a shorter time; and the activity and function of the stem cells can be maintained.

Description

Technical Field

[0001] The present invention relates to the technical field of cell separation and extraction, and specifically to a stem cell collection and extraction device. Background Technique

[0002] Hematopoietic stem cells, also known as hematopoietic stem substances, are a special type of stem cells. Stem cells have the potential for self-renewal and multi-directional differentiation and have extremely broad application prospects in the field of medical health, such as for treating various diseases, tissue engineering, and regenerative medicine. For example, mesenchymal stem cells can be used to treat osteoarthritis, autoimmune diseases, etc. To achieve these applications, it is necessary to collect and extract stem cells from different sources, which has promoted the research and improvement of stem cell collection and extraction devices. And the separation and extraction work of stem cells after collection is an important link for the subsequent utilization of stem cells.

[0003] When the current stem cell collection and extraction device is in use, blood and a neutralization reagent are mixed and placed in an EP tube, and then separated by a centrifuge. Then, the waste liquid is manually sucked with a pipette gun to leave the stem cells. And in order to ensure the effective separation of the supernatant and the precipitate, it is often necessary to perform repeated separation on the precipitate multiple times subsequently. This will make the operation procedures complex and scattered, require the cooperation of multiple staff members, involve a large amount of labor costs, and have low time efficiency. At the same time, centrifuging the stem cells multiple times will cause mechanical damage to the stem cells to a certain extent, which is not conducive to maintaining the activity and function of the stem cells.

[0004] In view of the above problems, a stem cell collection and extraction device is proposed. Summary of the Invention

[0005] The purpose of the present invention is to provide a stem cell collection and extraction device. By using this device for work, it solves the problems in the above background that when the current stem cell collection and extraction device is in use, blood and a neutralization reagent are mixed and placed in an EP tube, and then separated by a centrifuge. Then, the waste liquid is manually sucked with a pipette gun to leave the stem cells. And in order to ensure the effective separation of the supernatant and the precipitate, it is often necessary to perform repeated separation on the precipitate multiple times subsequently. This will make the operation procedures complex and scattered, require the cooperation of multiple staff members, involve a large amount of labor costs, and have low time efficiency. At the same time, centrifuging the stem cells multiple times will cause mechanical damage to the stem cells to a certain extent, which is not conducive to maintaining the activity and function of the stem cells.

[0006] To achieve the above purpose, the present invention provides the following technical solution: A stem cell collection and extraction device includes a working chassis and a feeding mechanism. In the middle of the lower part inside the working chassis, a power mechanism for energy extraction is provided, and above the power mechanism, a matching mechanism for driven centrifugation is provided. The feeding mechanism for efficient extraction is arranged on the outer periphery of the bottom of the matching mechanism;

[0007] The blanking mechanism includes a rotary joint arranged below the outer side of the matching mechanism. A first solenoid valve is installed in the middle below the rotary joint, and a first feed pipe is connected below the first solenoid valve. One side below the first feed pipe is connected with a first cyclone tube. A second solenoid valve is installed on one side of the rotary joint, and a shunt pipe is connected to the other side of the second solenoid valve. The bottom of the shunt pipe is connected with a liquid storage tank. The middle above the first cyclone tube is connected with a first overflow pipe, and the first cyclone tube is connected with a second feed pipe below. One side below the second feed pipe is connected with a second cyclone tube, and the middle above the second cyclone tube is connected with a second overflow pipe. The middle below the second cyclone tube is connected with a blanking pipe, and a storage bin is arranged below the blanking pipe. A liquid outlet is arranged below the outer side of the storage bin.

[0008] Further, the rotary joint is communicated with the first solenoid valve, the first feed pipe and the first cyclone tube, and the first cyclone tube is communicated with the first overflow pipe and the liquid storage tank. The first cyclone tube is communicated with the second feed pipe, the second cyclone tube, the blanking pipe and the storage bin.

[0009] Further, a control panel is installed in the middle of the outer surface of the working machine case. The power mechanism includes a lifting frame, an electric push cylinder, a driving gear and a rotating disk. An electric push cylinder is installed below the inner side of the lifting frame, and a driving gear is arranged above the electric push cylinder. A rotating disk is installed in the middle of the upper surface of the driving gear.

[0010] Further, the matching mechanism includes a support disk, a matching tooth groove, a support sliding frame and a guide rail. A matching tooth groove is opened in the middle of the lower surface of the support disk, and support sliding frames are arranged on both outer sides of the support disk. A guide rail is arranged in the middle of the rear of the support sliding frame. The support disk and the matching tooth groove are slidably connected with the support sliding frame through the driving gear, and the support sliding frame is slidably connected with the working machine case through the guide rail.

[0011] Further, the matching mechanism further includes a bearing seat and a sun gear. A bearing seat is installed on the lower surface of the support disk, and a sun gear is installed below the bearing seat. The sun gear is rotatably connected with the support disk through the bearing seat.

[0012] Further, the matching mechanism further includes a planetary gear and a connecting cylinder. A planetary gear is meshed with the outer side of the sun gear, and a connecting cylinder is installed in the middle of the planetary gear. The planetary gear is rotatably connected with the support disk.

[0013] Further, a material receiving mechanism for storing material extraction is arranged above the matching mechanism. The material receiving mechanism includes a centrifugal material pipe, a sliding rail, a plug-in end and a sealing piece. Sliding rails are fixed on both outer sides of the outer surface of the centrifugal material pipe, and a plug-in end is arranged in the middle below the centrifugal material pipe. A sealing piece is arranged on the outer surface of the plug-in end.

[0014] Further, the material receiving mechanism further includes a feeding pipe, a liquid adding pipe, and a liquid guiding pipe. A feeding pipe is arranged above the centrifugal material pipe, and a liquid adding pipe is arranged on one side of the feeding pipe. Liquid guiding pipes are respectively connected above the feeding pipe and the liquid adding pipe.

[0015] Further, a negative pressure mechanism for accelerating the flow is arranged on one side below the material discharging mechanism. The negative pressure mechanism includes a piston cylinder, a piston disc, a connecting rod, and a sliding sleeve frame. A piston disc is arranged below the interior of the piston cylinder, the middle part above the piston disc is connected with a connecting rod, and a sliding sleeve frame is arranged on one side above the connecting rod. The piston disc and the connecting rod are connected with the piston cylinder through a lifting frame and the sliding sleeve frame for lifting, and the connecting rod is rotationally connected with the lifting frame through the sliding sleeve frame.

[0016] Further, the negative pressure mechanism further includes a third electromagnetic valve and a ventilation valve. The third electromagnetic valve is installed in the middle of the lower part of the piston cylinder, and ventilation valves are installed on the upper and lower outer surfaces of the piston cylinder. The piston cylinder is communicated with the storage tank through the third electromagnetic valve.

[0017] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0018] 1. Through the material discharging mechanism, the present invention can realize the continuous action of multiple groups of cyclone separation structures, and can more effectively separate stem cells from other substances. The multiple groups of cyclone separation structures can perform multiple-stage separation on the liquid during the centrifugation process. Each stage of the cyclone separator can preliminarily separate and enrich the components in the liquid, so that the stem cells can reach a high purity in a short time. Compared with manually sucking the waste liquid with a pipette and centrifuging and purifying repeatedly, the overall separation time can be effectively shortened, and the separation purity and efficiency can be improved. At the same time, because the cyclone separation structure separates by using the natural flow and centrifugal force of the liquid, the separation method is relatively gentle, avoiding the damage of excessive shear force or impact force to the stem cells, and thus is beneficial to maintaining the activity and function of the stem cells.

[0019] 2. Through the power mechanism, the cooperation mechanism, and the material receiving mechanism, the present invention can complete the preparation work of multiple samples at the same time, greatly reducing the overall processing time. Especially when processing a large number of samples, the efficiency improvement is more significant. And the operation reduces the chance of manual frequent contact with the samples and reagents, reduces the pollution risk of external factors to the samples, helps to maintain the purity of the samples, and improves the success rate of stem cell extraction. At the same time, the structure of the planetary gear system is relatively more stable compared with a single centrifugal turntable, and can maintain good balance and stability during high-speed rotation, reducing the vibration and noise of the equipment.

[0020] 3. Through the negative pressure mechanism, the present invention can automatically generate negative pressure in the storage tank during the operation of the device, prompting the fluid to pass through the cyclone tube faster and with a certain flow rate, thereby increasing the flow rate of stem cell separation, ensuring the separation efficiency and effect. At the same time, the separation process is accelerated, and the chance of the sample being contaminated by microorganisms or other impurities is correspondingly reduced, which helps to obtain higher-quality stem cells. Moreover, the automatic negative pressure makes the automation degree of the entire stem cell preparation process higher, avoiding the use of other power equipment. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 is a schematic diagram of the overall external three-dimensional structure of the present invention;

[0022] Figure 2 is a schematic diagram of the internal three-dimensional structure of the working chassis of the present invention;

[0023] Figure 3 of the present invention Figure 2 is a schematic diagram of the right-side three-dimensional structure;

[0024] Figure 4 is a schematic diagram of the separated three-dimensional structure of the power mechanism and the matching mechanism of the present invention;

[0025] Figure 5 of the present invention Figure 4 is a schematic diagram of the bottom three-dimensional structure;

[0026] Figure 6 is a schematic diagram of the internal three-dimensional structure of the connection tube in cross-section of the present invention;

[0027] Figure 7 is a schematic diagram of the three-dimensional structure of the blanking mechanism of the present invention;

[0028] Figure 8 of the present invention Figure 7 is a schematic diagram of the left-side three-dimensional structure;

[0029] Figure 9 is a schematic diagram of the three-dimensional structure of the first cyclone tube in cross-section of the present invention;

[0030] Figure 10 is a schematic diagram of the partial cross-sectional three-dimensional structure of the piston cylinder of the present invention;

[0031] Figure 11 is a schematic diagram of the external three-dimensional structure of the piston cylinder of the present invention.

[0032] In the figure: 1. Working chassis; 2. Control panel; 3. Power mechanism; 301. Lifting frame; 302. Electric push cylinder; 303. Driving gear; 304. Rotating disk; 4. Matching mechanism; 401. Support disk; 402. Matching tooth grooves; 403. Support sliding frame; 404. Guide rail; 405. Bearing seat; 406. Sun gear; 407. Planet gear; 408. Connecting cylinder; 5. Feeding mechanism; 501. Centrifugal feed pipe; 502. Sliding rail; 503. Insertion end; 504. Sealing piece; 505. Feeding pipe; 506. Liquid adding pipe; 507. Liquid guiding pipe; 6. Discharging mechanism; 601. Rotary joint; 602. First solenoid valve; 603. First feed pipe; 604. First cyclone tube; 605. Second solenoid valve; 606. Diverging pipe; 607. Liquid storage tank; 608. First overflow pipe; 609. Second feed pipe; 610. Second cyclone tube; 611. Second overflow pipe; 612. Discharging pipe; 613. Stock storage tank; 614. Liquid outlet; 7. Negative pressure mechanism; 701. Piston cylinder; 702. Piston disk; 703. Connecting rod; 704. Sliding sleeve frame; 705. Third solenoid valve; 706. Vent valve. Detailed implementation manners

[0033] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0034] To solve the technical problems of time-consuming and easy contamination in the operations of adding materials and auxiliary liquids to the centrifugal feed pipes 501 one by one, as Figures 1 - 6 shown, the following preferred technical solutions are provided: A stem cell collection and extraction device includes a working chassis 1 and a discharging mechanism 6 arranged around the bottom periphery of the matching mechanism 4. In the middle of the lower part inside the working chassis 1, a power mechanism 3 is provided. In the middle of the outer surface of the working chassis 1, a control panel 2 is installed. The power mechanism 3 includes a lifting frame 301 installed at the bottom inside the working chassis 1. The lifting frame 301 is composed of a lower fixed cylinder and an upper lifting sleeve. An electric push cylinder 302 is installed below the inside of the lifting frame 301, and a driving gear 303 is arranged above the electric push cylinder 302. The driving gear 303 is driven to rotate by a servo-type motor, and the motor is installed in the upper lifting sleeve of the lifting frame 301. In the middle of the upper surface of the driving gear 303, a rotating disk 304 is installed. Through the power mechanism 3, power can be provided for the liquid addition and centrifugation of the device;

[0035] Above the power mechanism 3, there is a matching mechanism 4. The matching mechanism 4 includes a support disk 401 arranged in the middle inside the working chassis 1. In the middle of the lower surface of the support disk 401, there is a matching tooth groove 402. On both outer sides of the support disk 401, there are four groups of support sliding frames 403. In the middle at the rear of the support sliding frame 403, there is a sliding guide rail 404. The support disk 401 and the matching tooth groove 402 are slidably connected to the support sliding frame 403 through the driving gear 303, and the support sliding frame 403 is slidably connected to the working chassis 1 through the guide rail 404. Through the support sliding frame 403, while supporting the support disk 401, it can also ensure the radial rotation of the support disk 401. Through the guide rail 404, when the support disk 401 moves up and down, it can ensure the synchronous lifting of the support sliding frame 403 and the support disk 401;

[0036] On the lower surface of the support disk 401, there is a bearing seat 405. Below the bearing seat 405, there is a sun gear 406. And the sun gear 406 is rotatably connected to the support disk 401 through the bearing seat 405. On the outer side of the sun gear 406, there is a planet gear 407 meshed. And in the middle of the planet gear 407, there is a connecting cylinder 408. The planet gear 407 is a miniaturized sun gear 406. On the outer side of its upper surface, there is also a rotatable bearing between it and the support disk 401. And the center of the planet gear 407 is fixedly penetrated by the connecting cylinder 408, and the connecting cylinder 408 penetrates through the support disk 401. The planet gear 407 is rotatably connected to the support disk 401. Through the sun gear 406, when the support disk 401 rotates, it can drive the planet gear 407 and the connecting cylinder 408 to rotate synchronously to realize the conversion from low speed to high speed;

[0037] Above the matching mechanism 4, there is a material receiving mechanism 5. The material receiving mechanism 5 includes a centrifugal material pipe 501 arranged inside the connecting cylinder 408. On both outer sides of the outer surface of the centrifugal material pipe 501, there are fixed sliding rails 502. And in the middle below the centrifugal material pipe 501, there is a plug-in end 503. On the outer surface of the plug-in end 503, there is a sealing piece 504. Through the plug-in end 503 communicated with the centrifugal material pipe 501 and the matching sealing piece 504, the centrifugal material pipe 501 can be hermetically plugged on the connecting cylinder 408 to realize the connection between the centrifugal material pipe 501 and the bottom, facilitating the subsequent circulation and separation of the centrifuged liquid;

[0038] Above the centrifugal material pipe 501, there is a feeding pipe 505. And on one side of the feeding pipe 505, there is a liquid adding pipe 506. The feeding pipe 505 and the liquid adding pipe 506 are radially distributed with respect to the position of the centrifugal material pipe 501 on the support disk 401. Above the feeding pipe 505 and the liquid adding pipe 506, there are respectively connected with liquid guide pipes 507. The liquid guide pipe 507 of the feeding pipe 505 can introduce the collected blood into the centrifugal material pipe 501, and the liquid guide pipe 507 of the liquid adding pipe 506 can add the auxiliary sedimentation solution into the centrifugal material pipe 501;

[0039] Through the electric push rod 302, the upper section of the lifting frame 301 and the driving gear 303 can be driven to move up and down. When the driving gear 303 moves upward, by using the arc chamfer on the tooth surface and the rotation adjustment of the servo motor of the driving gear 303, it can be meshed with the mating tooth groove 402. When the driving gear 303 continues to move upward under the thrust, the support plate 401 can be automatically lifted under the action of the support carriage 403 and the guide rail 404, so that multiple sets of centrifugal tubes 501 are aligned with the corresponding feeding tubes 505, enabling the collected blood to be introduced. Subsequently, the electric push rod 302 drives the driving gear 303 to move downward, and the support plate 401 moves downward together under the action of gravity. At this time, the driving gear 303 rotates, causing the support plate 401 to rotate accordingly under the action of the mating tooth groove 402, so that the centrifugal tubes 501 rotate to the lower side of the liquid adding tube 506. Then, the support plate 401 is lifted again, and the auxiliary sedimentation solution is added into the centrifugal tubes 501 by using the liquid adding tube 506. Then, the support plate 401 is moved downward to reset. This process can synchronously perform feeding and liquid adding operations on multiple sets of centrifugal tubes 501, complete the preparation work of multiple samples at the same time, greatly reduce the overall processing time, especially when processing a large number of samples, the efficiency improvement is more significant, and the operation reduces the chance of frequent manual contact with samples and reagents, reduces the risk of sample contamination by external factors, helps maintain the purity of the samples, and improves the success rate of stem cell extraction;

[0040] At this time, the electric push rod 302 moves the driving gear 303 downward to mesh with the sun gear 406. By using the rotation of the driving gear 303 to drive the sun gear 406, the rotational force can be transmitted to the planetary gear 407. At this time, the planetary gear 407 drives the connecting cylinder 408 and the centrifugal tubes 501 to rotate, so as to perform centrifugal separation on the liquid in the centrifugal tubes 501, ensure that all samples are processed under the same centrifugal force field, and achieve efficient and uniform synchronous centrifugation. The transmission ratio between the sun gear 406 and the planetary gear 407 is fixed. By controlling the rotation speed of the sun gear 406, the rotation speed of the centrifugal tubes 501 on each planetary gear 407 can be accurately controlled. Applying a lower rotation speed to the sun gear 406 can make the planetary gear 407 have a high rotation speed under the action of the transmission ratio, avoiding the high consumption of directly applying a high rotation speed to make the centrifugal tubes 501 rotate for centrifugation. At the same time, the structure of the planetary gear 407 system is relatively stable compared with a single centrifugal turntable, and it can maintain good balance and stability during high-speed rotation, reducing the vibration and noise of the equipment.

[0041] To solve the technical problems that multiple re-separations of the precipitation solution are required, which will make the operation procedures complex and scattered, require the cooperation of multiple staff, involve a large amount of labor costs, and have low time efficiency. At the same time, multiple centrifugal separations of stem cells will cause mechanical damage to the stem cells to a certain extent, such as Figures 1 - 4 andFigures 7 - 9 As shown in the figure, the following preferred technical solutions are provided: A first solenoid valve 602 is installed in the middle below the rotary joint 601, and a first feed pipe 603 is connected below the first solenoid valve 602. One side below the first feed pipe 603 is connected to a first cyclone tube 604. The rotary joint 601 is communicated with the first solenoid valve 602, the first feed pipe 603, and the first cyclone tube 604. A second solenoid valve 605 is installed on one side of the rotary joint 601, and a shunt pipe 606 is connected to the other side of the second solenoid valve 605. The bottom of the shunt pipe 606 is connected to a liquid storage tank 607. Through the rotary joint 601 with the existing principle, when the connecting cylinder 408 rotates, the joint can have the ability of rotary separation, avoiding the chaos of the lower mechanism rotating with the connecting cylinder 408;

[0042] A first overflow pipe 608 is connected to the middle above the first cyclone tube 604. The first cyclone tube 604 is communicated with the first overflow pipe 608 and the liquid storage tank 607. And a second feed pipe 609 is connected below the first cyclone tube 604. One side below the second feed pipe 609 is connected to a second cyclone tube 610. And a second overflow pipe 611 is connected to the middle above the second cyclone tube 610. The middle below the second cyclone tube 610 is connected to a blanking pipe 612. And a storage tank 613 is arranged below the blanking pipe 612. A liquid outlet 614 is arranged below the outer side of the storage tank 613. The first cyclone tube 604 is communicated with the second feed pipe 609, the second cyclone tube 610, the blanking pipe 612, and the storage tank 613. Through the first cyclone tube 604 and the second cyclone tube 610, whose internal structure is the same as that of the existing cyclone, the precipitation liquid and the mixed liquid mixed with a certain amount of clear liquid are tangentially and stepwise fed into the first cyclone tube 604 and the second cyclone tube 610 with a certain pressure by the first feed pipe 603 and the second feed pipe 609, generating a high-speed rotating flow field in the cylindrical cavity. The components with a large density in the mixture move downward along the axis and outward along the radius under the action of the swirling flow field. When reaching the conical section, they move downward along the wall of the device and are discharged from the underflow port, thus forming an outer swirling flow field. The components with a small density move toward the central axis direction and form an upward moving inner vortex in the center of the axis, and then are discharged from the first overflow pipe 608 and the second overflow pipe 611 respectively, thus achieving the purpose of two-phase separation;

[0043] After centrifugal separation, by closing the second solenoid valve 605 and opening the first solenoid valve 602, the mixed liquid of the sediment liquid and part of the supernatant at the bottom of the centrifugal feed pipe 501 can be flushed into the first cyclone pipe 604 through the first feed pipe 603. Then, the first solenoid valve 602 is closed and the second solenoid valve 605 is opened, allowing the remaining supernatant to flow into the liquid storage tank 607 through the shunt pipe 606. At this time, by using the cyclone separation of the first cyclone pipe 604, the denser stem cell sediment liquid continues to flow downward in the first cyclone pipe 604, and the less dense supernatant is allowed to flow into the liquid storage tank 607 through the first overflow pipe 608. Subsequently, the stem cell sediment liquid is tangentially introduced into the second cyclone pipe 610 through the second feed pipe 609 to perform another cyclone separation treatment on the sediment liquid. Finally, the separated stem cell sediment liquid is stored in the storage tank 613 through the discharge pipe 612, and a small amount of the separated supernatant is continuously introduced into the liquid storage tank 607 through the second overflow pipe 611 to complete the extraction of the separated stem cell sediment liquid. In this way, through the continuous action of multiple sets of cyclone separation structures, the stem cells can be more effectively separated from other substances. The multiple sets of cyclone separation structures can perform multiple-stage separation on the liquid during centrifugation. Each stage of the cyclone separator can preliminarily separate and enrich the components in the liquid, enabling the stem cells to reach a higher purity in a shorter time. Compared with manually sucking the waste liquid with a pipette and repeatedly centrifuging and purifying, it can effectively shorten the overall separation time, improve the separation purity and efficiency. At the same time, because the cyclone separation structure uses the natural flow and centrifugal force of the liquid for separation, it avoids the excessive shear force or impact force caused by repeatedly applying external centrifugal force to damage the stem cells, thereby being beneficial to maintaining the activity and function of the stem cells.

[0044] To solve the technical problems of slow flow rate and poor separation effect, as Figures 1 - 5 and Figure 10 and Figure 11 shown, the following preferred technical solutions are provided:

[0045] A negative pressure mechanism 7 for accelerating the flow is arranged on one side below the feeding mechanism 6. The negative pressure mechanism 7 includes a piston cylinder 701 connected to one side above the storage tank 613. A piston disk 702 is arranged below the interior of the piston cylinder 701. The middle part above the piston disk 702 is connected with a connecting rod 703. One side above the connecting rod 703 is provided with a sliding sleeve 704. And the piston disk 702 and the connecting rod 703 are connected with the piston cylinder 701 for lifting through a lifting frame 301 and the sliding sleeve 704. The connecting rod 703 is rotationally connected with the lifting frame 301 through the sliding sleeve 704. By driving the piston disk 702 to move upward through the lifting frame 301, the piston cylinder 701 can pump air from the storage tank 613, causing negative pressure inside the storage tank 613 when the liquid outlet 614 is closed by a valve.

[0046] A third solenoid valve 705 is installed in the middle of the lower part of the piston cylinder 701, and air vent valves 706 are installed on the upper and lower outer surfaces of the piston cylinder 701. The piston cylinder 701 is connected to the storage tank 613 through the third solenoid valve 705, through the third solenoid valve 705;

[0047] Through the connecting rod 703, when the upper section of the lifting frame 301 rises, the piston disc 702 can be driven to move upward in the piston cylinder 701, and the lower air vent valve 706 is closed to evacuate the storage tank 613. Through the third solenoid valve 705, it can be closed after the upward evacuation. At this time, the air vent valve 706 is opened to exhaust the air pressure in the piston cylinder 701, so that when the piston disc 702 moves downward due to the lifting frame 301, the negative pressure in the storage tank 613 will not disappear. In this way, a negative pressure is automatically generated in the storage tank 613 during the operation of the device, prompting the fluid to pass through the cyclone tube faster and at a certain flow rate, thereby increasing the flow rate of stem cell separation, ensuring the separation efficiency and effect. At the same time, the separation process is accelerated, and the chance of the sample being contaminated by microorganisms or other impurities is correspondingly reduced, which helps to obtain higher-quality stem cells. Moreover, the automatic negative pressure makes the automation degree of the entire stem cell preparation process higher, avoiding the use of other power equipment.

[0048] It should be noted that in this article, relational terms such as first and second are only used to distinguish one entity or operation from another entity or operation, and do not necessarily require or imply any actual relationship or order between these entities or operations. Moreover, the term "comprising", "including" or any other variant thereof is intended to cover non-exclusive inclusion, so that a process, method, article or device including a series of elements not only includes those elements, but also includes other elements not expressly listed, or also includes elements inherent to such process, method, article or device.

[0049] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions and variations can be made to these embodiments without departing from the principle and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. A stem cell collection and extraction device, comprising a working chassis (1) and a blanking mechanism (6), characterized in that: In the middle of the lower part inside the working chassis (1), a power mechanism (3) for energy extraction is provided, and above the power mechanism (3), a matching mechanism (4) for driven centrifugation is provided. The feeding mechanism (6) for efficient extraction is arranged on the outer periphery of the bottom of the matching mechanism (4). The feeding mechanism (6) includes a rotary joint (601) arranged below the outside of the matching mechanism (4). In the middle of the lower part of the rotary joint (601), a first solenoid valve (602) is installed. Below the first solenoid valve (602), a first feed pipe (603) is connected. On one side of the lower part of the first feed pipe (603), a first cyclone tube (604) is connected. On one side of the rotary joint (601), a second solenoid valve (605) is installed, and on the other side of the second solenoid valve (605), a shunt pipe (606) is connected. The bottom of the shunt pipe (606) is connected to a liquid storage tank (607). In the middle of the upper part of the first cyclone tube (604), a first overflow pipe (608) is connected. Below the first cyclone tube (604), a second feed pipe (609) is connected. On one side of the lower part of the second feed pipe (609), a second cyclone tube (610) is connected. In the middle of the upper part of the second cyclone tube (610), a second overflow pipe (611) is connected. In the middle of the lower part of the second cyclone tube (610), a feed pipe (612) is connected. Below the feed pipe (612), a storage tank (613) is provided. On the outer lower part of the storage tank (613), a liquid outlet (614) is provided.

2. The stem cell collection and extraction device according to claim 1, wherein: The rotary joint (601) is communicated with the first solenoid valve (602), the first feed pipe (603), and the first cyclone tube (604). The first cyclone tube (604) is communicated with the first overflow pipe (608) and the liquid storage tank (607). The first cyclone tube (604) is communicated with the second feed pipe (609), the second cyclone tube (610), the feed pipe (612), and the storage tank (613).

3. The stem cell collection and extraction device according to claim 1, wherein: In the middle of the outer surface of the working chassis (1), a control panel (2) is installed. The power mechanism (3) includes a lifting frame (301), an electric push cylinder (302), a driving gear (303), and a rotating disk (304). Inside the lower part of the lifting frame (301), the electric push cylinder (302) is installed. Above the electric push cylinder (302), the driving gear (303) is provided. In the middle of the upper surface of the driving gear (303), the rotating disk (304) is installed.

4. The stem cell collection and extraction device according to claim 3, characterized in that: The mating mechanism (4) includes a support disk (401), mating tooth grooves (402), a support carriage (403), and a guide rail (404). In the middle of the lower surface of the support disk (401), mating tooth grooves (402) are provided. On both outer sides of the support disk (401), support carriages (403) are arranged. In the middle of the rear of the support carriage (403), a guide rail (404) is provided. The support disk (401) and the mating tooth grooves (402) are slidably connected to the support carriage (403) through a driving gear (303), and the support carriage (403) is slidably connected to the working machine case (1) through the guide rail (404).

5. The stem cell collection and extraction device according to claim 4, characterized in that: The mating mechanism (4) further includes a bearing seat (405) and a sun gear (406). A bearing seat (405) is installed on the lower surface of the support disk (401), and a sun gear (406) is installed below the bearing seat (405). The sun gear (406) is rotatably connected to the support disk (401) through the bearing seat (405).

6. The stem cell collection and extraction device according to claim 5, characterized in that: The mating mechanism (4) further includes a planetary gear (407) and a connecting cylinder (408). A planetary gear (407) is meshed with the outer side of the sun gear (406). In the middle of the planetary gear (407), a connecting cylinder (408) is installed. The planetary gear (407) is rotatably connected to the support disk (401).

7. A stem cell collection and extraction device according to claim 1, characterized in that: Above the mating mechanism (4), a material receiving mechanism (5) for storing and extracting materials is provided. The material receiving mechanism (5) includes a centrifugal material pipe (501), a sliding rail (502), a plug-in end (503), and a sealing piece (504). On both outer sides of the outer surface of the centrifugal material pipe (501), sliding rails (502) are fixed. In the middle of the lower part of the centrifugal material pipe (501), a plug-in end (503) is provided. A sealing piece (504) is arranged on the outer surface of the plug-in end (503).

8. The stem cell collection and extraction device according to claim 7, characterized in that: The material receiving mechanism (5) further includes a feeding pipe (505), a liquid adding pipe (506), and a liquid guiding pipe (507). Above the centrifugal material pipe (501), a feeding pipe (505) is provided. On one side of the feeding pipe (505), a liquid adding pipe (506) is provided. Above the feeding pipe (505) and the liquid adding pipe (506), liquid guiding pipes (507) are respectively connected.

9. The stem cell collection and extraction device according to claim 1, wherein: On one side below the material discharging mechanism (6), a negative pressure mechanism (7) for accelerating the flow is provided. The negative pressure mechanism (7) includes a piston cylinder (701), a piston disk (702), a connecting rod (703), and a sliding sleeve (704). Below the inner part of the piston cylinder (701), a piston disk (702) is provided. In the middle of the upper part of the piston disk (702), a connecting rod (703) is connected. On one side of the upper part of the connecting rod (703), a sliding sleeve (704) is provided. The piston disk (702) and the connecting rod (703) are connected to the piston cylinder (701) for lifting through a lifting frame (301) and the sliding sleeve (704). The connecting rod (703) is rotatably connected to the lifting frame (301) through the sliding sleeve (704).

10. A stem cell collection and extraction device according to claim 9, characterized in that: The negative pressure mechanism (7) further includes a third solenoid valve (705) and a ventilation valve (706). The third solenoid valve (705) is installed in the middle below the piston cylinder (701), and the ventilation valves (706) are installed on the upper and lower outer surfaces of the piston cylinder (701). The piston cylinder (701) is connected to the storage tank (613) through the third solenoid valve (705).

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