Automatic column passing machine and using method thereof

Abstract

The invention relates to the technical field of column passing, and discloses an automatic column passing machine and a using method thereof.The automatic column passing machine comprises a shell, a mounting plate is fixedly connected to the interior of the shell, an evaporation kettle is rotatably connected to the interior of the mounting plate, a first motor is mounted in the shell, and a first bevel gear is fixedly arranged at the output end of the first motor; the outer wall of the first bevel gear is rotationally connected into the shell, the tooth end of the first bevel gear is connected with a second bevel gear and a third bevel gear in a meshed mode, the outer wall of the second bevel gear and the outer wall of the third bevel gear are rotationally connected into the shell, and the outer wall of the third bevel gear is fixedly connected with a first rotating shaft. By arranging the first motor for driving and by means of the first bevel gear, the second bevel gear, the third bevel gear, the first rotating shaft, the second rotating shaft, the transmission mechanism, the belt mechanism and other transmission components, the functions that the evaporation kettle rotates to be heated evenly and meanwhile drives the rotating frame and the stirring rods on the rotating frame to rotate for stirring are achieved, and then the product quality is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of column chromatography, and specifically relates to an automatic column chromatography machine and a using method thereof. Background Art

[0002] In the work of detecting various foods, it is also necessary to detect toxins, additives, etc. in the foods; before detection, column chromatography work must be carried out. In the prior art, the process of column chromatography depends entirely on manual operation, which is time-consuming and laborious. If it encounters a sample that is difficult to pass through the column, it may not be able to finish passing through the column in a day. The existing column chromatography machines on the market are difficult to operate in the whole process and require manual intervention, and the degree of automation of the operation is insufficient, which affects the operation accuracy and operation efficiency; for this reason, an automatic column chromatography machine is needed.

[0003] In the design process of the automatic column chromatography machine, how to achieve efficient and uniform evaporation and mixing operations is one of the key technical problems. The evaporation operation is an essential link in many chemical processes, such as in solvent recovery, concentrated solution, etc. To ensure the efficiency and uniformity of the evaporation process, it is necessary to make the substances in the evaporation container fully heated. At the same time, during the evaporation process or subsequent dissolution process, the stirring operation can promote the mixing and reaction of substances, improving the processing efficiency and quality. In the related technologies of the existing automatic column chromatography machines, there are certain limitations in the design of the evaporation and stirring structures of some devices. The evaporation containers of some devices are unevenly heated, affecting the evaporation effect. Summary of the Invention

[0004] Aiming at the deficiencies of the prior art, the present invention provides an automatic column chromatography machine and a using method thereof, which solve the problem that the evaporation container in the automatic column chromatography machine is unevenly heated, affecting the evaporation effect.

[0005] To achieve the above objectives, the present invention is realized through the following technical solutions: An automatic column chromatography machine includes a housing, an installation plate is fixedly connected inside the housing, an evaporation kettle is rotatably connected inside the installation plate, a first motor is installed inside the housing, a first bevel gear is fixedly arranged at the output end of the first motor, the outer wall of the first bevel gear is rotatably connected inside the housing, the tooth end of the first bevel gear is meshed with a second bevel gear and a third bevel gear, the outer walls of the second bevel gear and the third bevel gear are rotatably connected inside the housing, a first rotating shaft is fixedly connected to the outer wall of the third bevel gear, a second rotating shaft is fixedly connected to the outer wall of the second bevel gear, the second rotating shaft is connected with a transmission mechanism, the transmission mechanism is connected with the evaporation kettle, a belt mechanism is connected to the outer wall of the first rotating shaft, the belt mechanism is connected with a rotating frame, the outer wall of the rotating frame is rotatably connected inside the housing, a cleaning mechanism and a liquid discharging mechanism are arranged on the outer wall of the rotating frame, a stirring rod is fixedly connected to the outer wall of the rotating frame, and a heating assembly is arranged inside the housing.

[0006] Through the above solution: There are upper and lower groups of evaporation kettles. The first motor drives the first bevel gear, which in turn drives the second and third bevel gears to rotate in opposite directions. The second bevel gear drives the evaporation kettle to rotate through the second rotating shaft and the transmission mechanism, ensuring uniform heating, which is beneficial to improving the efficiency and quality of operations such as evaporation or dissolution. The third bevel gear drives the rotating frame and the stirring rod to rotate through the first rotating shaft and the belt mechanism. The stirring rod can assist both evaporation and dissolution operations, enhancing the functionality and applicability of the equipment.

[0007] Preferably, the heating component includes a first bracket, the outer wall of the first bracket is fixedly connected inside the housing, and a heating rack is fixedly connected to the outer wall of the first bracket.

[0008] Preferably, the transmission mechanism includes a circular gear, the inside of the circular gear is fixedly connected to the outer wall of the second rotating shaft, the tooth end of the circular gear is meshed with a toothed ring, and the inside of the toothed ring is fixedly connected to the outer wall of the evaporation kettle.

[0009] Preferably, the belt mechanism includes a transmission belt. The outer wall of the first pulley is fixedly connected to the outer wall of the first rotating shaft. The first pulley is connected to a transmission belt, and the transmission belt is connected to a second pulley. The inside of the second pulley is fixedly connected to the outer wall of the rotating frame.

[0010] Preferably, the cleaning mechanism includes a first motor, the outer wall of the first motor is fixedly connected to the outer wall of the rotating frame, the output end of the first motor is fixedly provided with a first lead screw, the outer wall of the first lead screw is threadedly connected to a first sliding frame, the inside of the first sliding frame is slidably connected to a sliding rod, both ends of the sliding rod are fixedly connected to the outer wall of the rotating frame, the outer wall of the first sliding frame is fixedly connected to a cleaning brush, and the inner wall of the cleaning brush is slidably connected to the outer wall of the stirring rod.

[0011] Preferably, the liquid discharging mechanism includes a water tank, the water tank is communicated with the rotating frame, the water tank is fixed inside the housing, and a nozzle is arranged on the outer wall of the rotating frame.

[0012] Preferably, an electric slide rail is arranged inside the housing, a pipette is slidably connected inside the electric slide rail, a storage rack is installed inside the housing, a storage tank is arranged inside the housing, and a feeding pipe is arranged on the lower surface of the storage tank.

[0013] Preferably, an electric push rod is installed inside the housing, the output end of the electric push rod is fixedly connected to an electric gripper, a fixing frame is arranged on the outer wall of the electric gripper, and a through-column cup is fixedly connected to the inner wall of the fixing frame.

[0014] Preferably, a second support is fixedly connected to the outer wall of the fixing frame. A second motor is fixedly connected to the outer wall of the second support. A second lead screw is fixedly arranged at the output end of the second motor. A second sliding frame is threadedly connected to the outer wall of the second lead screw. The outer wall of the second sliding frame is slidably connected to the inside of the second support. An installation ring is threadedly connected to the outer wall of the second sliding frame. A filter element is fixedly connected to the lower surface of the installation ring.

[0015] A method for using an automatic column chromatography machine includes the following steps: The raw materials are placed on the storage rack. First, the raw materials are transferred to the upper evaporation kettle through a pipette gun and heated and evaporated by the heating component. At the same time, the first motor is started, and through the bevel gear pair and the bevel gear pair respectively, the transmission mechanism and the belt mechanism are used to drive the evaporation kettle to rotate, and the stirring rod rotates in the opposite direction to the evaporation kettle; After evaporation, a dissolving solution is added, and then the stirring rod is driven to rotate through the transmission mechanism for stirring and dissolving; After dissolution, it is transferred to the column chromatography cup for column chromatography. The column chromatography cup is pushed to move by the electric push rod, and the storage tank adds the reagent for column chromatography into the column chromatography cup again; the column chromatography cup is moved to be close to the lower evaporation kettle to start column chromatography; The solution after column chromatography flows into the lower evaporation kettle and is evaporated to dryness again by the heating component. Similarly, the first motor is used for stirring to assist in evaporation to dryness; After evaporation to dryness, the required sample is obtained. The cleaning brush is sleeved on the outer wall of the stirring rod through the cleaning mechanism. While the first motor is started, the cleaning liquid is added through the liquid outlet mechanism to clean the evaporation kettle; the electric gripper releases the fixing frame, and the column chromatography cup and the second support are removed for waste treatment.

[0016] Working principle: Place the raw materials on the storage rack. After being sucked by the pipette gun, they are transferred to the upper evaporation kettle. The heating component is started, and the first support supplies power to the heating rack to heat the evaporation kettle. At the same time, the first motor operates. Through bevel gear transmission, the second bevel gear drives the second rotating shaft and the circular gear to rotate. The circular gear meshes with the tooth ring on the outer wall of the evaporation kettle to drive the evaporation kettle to rotate to achieve uniform heating; The third bevel gear drives the first rotating shaft to rotate. Through the belt mechanism, the rotating frame and the stirring rod rotate to stir the raw materials in the evaporation kettle to assist evaporation. After evaporation, a dissolving solution is added and stirred continuously to make it fully dissolve; The pipette gun transfers the solution to the column chromatography cup. The electric push rod moves the column chromatography cup to a suitable position, opens the valve of the storage tank to add the column chromatography reagent, and then moves the column chromatography cup to the side of the lower evaporation kettle. The second motor can adjust the height of the filter element, and the solution is filtered through the filter element to complete column chromatography; The solution after column chromatography flows into the lower evaporation kettle. The heating component heats and evaporates it again. The first motor drives the evaporation kettle and the stirring rod to rotate to assist evaporation to dryness. After evaporation to dryness, the valve at the bottom of the evaporation kettle is opened, and the powdered sample is put into the sample box; Start the liquid outlet mechanism motor 1. The lead screw 1 pushes the sliding frame 1 and the cleaning brush down to slide on the outer wall of the stirring rod. The motor 1 drives the stirring rod to rotate. The cleaning brush fits against the inner wall of the evaporation kettle for cleaning. At the same time, the spray head sprays the cleaning liquid to enhance the effect. After cleaning, the electric gripper releases the fixing frame, and the column passing cup and the support 2 are removed to process the waste. The motor 2 drives the lead screw 2 to rotate, pushing the sliding frame 2 and the filter element to lift and adjust the height. When replacing, the filter element is lifted to a suitable height, and the installation ring is rotated to remove the old filter element and replace it with a new one to ensure the filtering performance of the equipment.

[0017] The present invention provides an automatic column passing machine and its use method. It has the following beneficial effects: 1. The present invention is driven by setting the motor 1, and with the help of transmission components such as bevel gear 1, bevel gear 2, bevel gear 3, rotating shaft 1, rotating shaft 2, transmission mechanism and belt mechanism, the evaporation kettle rotates to be heated evenly, and at the same time drives the rotating frame and the stirring rod on it to rotate for stirring, thereby improving the product quality.

[0018] 2. The present invention further adds a cleaning mechanism and a liquid outlet mechanism. In the cleaning mechanism, the motor 1 drives the lead screw 1 to rotate, pushing the sliding frame 1 and the cleaning brush down to slide on the outer wall of the stirring rod. Then, with the rotation of the stirring rod, the cleaning brush fits against the inner wall of the evaporation kettle and rotates, effectively removing the residual substances on the inner wall. At the same time, the liquid outlet mechanism stores the cleaning liquid in the water tank, connects the spray head with the water tank through the pipe structure of the rotating frame, and the cleaning liquid is sprayed out through the spray head to further enhance the cleaning effect.

[0019] 3. The fixing frame of the present invention fixes the support 2, and the support 2 fixes the motor 2. Starting the motor 2 can drive the lead screw 2 to rotate, thereby pushing the sliding frame 2 to lift, realizing flexible adjustment of the height of the filter element. The contact position between the filter element and the solution can be quickly adjusted according to actual needs. At the same time, the filter element can be conveniently lifted out of the column passing cup, and the filter element can be disassembled and replaced by rotating the installation ring.

[0020] 4. The automatic column passing machine of the present invention realizes the full-process automated operation from raw material addition, heating evaporation, stirring and dissolving, column passing separation to final sample collection and equipment cleaning. After the raw materials are placed on the storage rack, the raw materials are automatically transferred to the upper evaporation kettle for heating evaporation by a pipette gun; after evaporation, the dissolving liquid is added and automatically stirred and dissolved; the dissolved solution is automatically transferred to the column passing cup for column passing, and at the same time, the column passing reagent is automatically added; the solution after column passing flows into the lower evaporation kettle and is evaporated to dryness again, and finally the required sample is obtained; after the sample collection is completed, the equipment is automatically cleaned, and components such as the column passing cup are removed for waste treatment. The whole process reduces excessive manual intervention and greatly improves the experimental efficiency and accuracy. Description of the Drawings

[0021] Figure 1 is a three-dimensional view of the present invention; Figure 2 Partial structural schematic diagram of the first rotating shaft of the present invention; Figure 3 Partial structural schematic diagram of the first motor of the present invention; Figure 4 Partial structural schematic diagram of the third bevel gear of the present invention; Figure 5 Partial structural schematic diagram of the fixing bracket of the present invention; Figure 6 Partial structural schematic diagram of the electric gripper of the present invention.

[0022] Wherein, 1, housing; 2, mounting plate; 3, evaporation kettle; 4, first motor; 5, first bevel gear; 6, second bevel gear; 7, third bevel gear; 8, first rotating shaft; 9, second rotating shaft; 10, transmission mechanism; 11, belt mechanism; 12, rotating frame; 13, stirring rod; 14, cleaning mechanism; 15, liquid discharging mechanism; 16, circular gear; 17, toothed ring; 18, first pulley; 19, transmission belt; 20, second pulley; 21, first motor; 22, first lead screw; 23, first sliding frame; 24, sliding rod; 25, cleaning brush; 26, water tank; 27, nozzle; 28, first bracket; 29, heating frame; 30, electric slide rail; 31, pipette; 32, storage rack; 33, storage tank; 34, feeding pipe; 35, electric push rod; 36, electric gripper; 37, fixing bracket; 38, column passing cup; 39, second bracket; 40, second motor; 41, second lead screw; 42, second sliding frame; 43, mounting ring; 44, filter element. Detailed implementation manners

[0023] Next, the technical solutions of the present invention will be clearly and completely described in conjunction with the accompanying drawings of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

[0024] Please refer to the attached Figure 1 - attached Figure 3, an embodiment of the present invention provides an automatic column chromatography machine, which includes a housing 1. Inside the housing 1, a mounting plate 2 is fixedly connected. Inside the mounting plate 2, an evaporation kettle 3 is rotatably connected. Inside the housing 1, a first motor 4 is installed. The output end of the first motor 4 is fixedly provided with a first bevel gear 5. The outer wall of the first bevel gear 5 is rotatably connected inside the housing 1. The tooth end of the first bevel gear 5 is meshed with a second bevel gear 6 and a third bevel gear 7. The outer walls of the second bevel gear 6 and the third bevel gear 7 are both rotatably connected inside the housing 1. The outer wall of the third bevel gear 7 is fixedly connected with a first rotating shaft 8. The outer wall of the second bevel gear 6 is fixedly connected with a second rotating shaft 9. The second rotating shaft 9 is connected with a transmission mechanism 10. The transmission mechanism 10 is connected with the evaporation kettle 3. The outer wall of the first rotating shaft 8 is connected with a belt mechanism 11. The belt mechanism 11 is connected with a rotating frame 12. The outer wall of the rotating frame 12 is rotatably connected inside the housing 1. The outer wall of the rotating frame 12 is provided with a cleaning mechanism 14 and a liquid discharging mechanism 15. The outer wall of the rotating frame 12 is fixedly connected with a stirring rod 13. Inside the housing 1, a heating component is provided.

[0025] Specifically, a valve is provided on the lower side of the evaporation kettle 3 for releasing the substances inside the evaporation kettle 3. A diversion pipe can also be added to guide the flow of the liquid. In this solution, two evaporation kettles 3 are provided, which are located on the upper and lower sides respectively. The evaporation kettles 3 on the upper and lower sides respectively correspond to a group of first motors 4. Starting the first motor 4 can drive the second bevel gear 6 and the third bevel gear 7 to rotate through the first bevel gear 5. The housing 1 supports the rotation of the first bevel gear 5, the second bevel gear 6 and the third bevel gear 7. The second bevel gear 6 and the third bevel gear 7 rotate in opposite directions. The second bevel gear 6 drives the second rotating shaft 9 to rotate, and the third bevel gear 7 drives the first rotating shaft 8 to rotate. The second rotating shaft 9 drives the evaporation kettle 3 to rotate through the transmission mechanism 10, which can make the evaporation kettle 3 heat evenly. The first rotating shaft 8 drives the rotating frame 12 to rotate through the belt mechanism 11, and the rotating frame 12 drives the stirring rod 13 to rotate, which can carry out stirring. Stirring can assist the evaporation work or the dissolution work.

[0026] Please refer to the appendix Figure 3 , the heating component includes a first support 28. The outer wall of the first support 28 is fixedly connected inside the housing 1. The outer wall of the first support 28 is fixedly connected with a heating frame 29.

[0027] Specifically, two sets of heating components are also provided, which respectively correspond to the evaporation kettles 3 on the upper and lower sides. Electric wires are arranged inside the first support 28. Resistance sheets are arranged at the positions where the heating frame 29 is in contact with the evaporation kettle 3. Heat is generated when an electric current passes through. The evaporation kettle 3 is heated through the heating frame 29. Struts can be added inside the housing 1 to fix the heating frame 29.

[0028] Please refer to the appendix Figure 3, the transmission mechanism 10 includes a circular gear 16. The inside of the circular gear 16 is fixedly connected to the outer wall of the second rotating shaft 9. The tooth end of the circular gear 16 is meshed and connected with a toothed ring 17. The inside of the toothed ring 17 is fixedly connected to the outer wall of the evaporation kettle 3.

[0029] Specifically, the second rotating shaft 9 drives the circular gear 16 to rotate. The circular gear 16 meshes and rotates with the toothed ring 17. The toothed ring 17 drives the evaporation kettle 3 to rotate. The mounting plate 2 supports the rotation of the evaporation kettle 3, and the power of the first motor 4 can be transmitted to the evaporation kettle 3.

[0030] Please refer to the appendix Figure 3 , the belt mechanism 11 includes a transmission belt 19. The outer wall of the first pulley 18 is fixedly connected to the outer wall of the first rotating shaft 8. The first pulley 18 is connected to the transmission belt 19. The transmission belt 19 is connected to the second pulley 20. The inside of the second pulley 20 is fixedly connected to the outer wall of the rotating frame 12.

[0031] Specifically, the first rotating shaft 8 drives the transmission belt 19 to rotate. The transmission belt 19 drives the first motor 21 to rotate through the second pulley 20. The first motor 21 drives the rotating frame 12 to rotate, thereby transmitting the power of the first motor 4 to the rotating frame 12.

[0032] Please refer to the appendix Figure 4 , the cleaning mechanism 14 includes a first motor 21. The outer wall of the first motor 21 is fixedly connected to the outer wall of the rotating frame 12. A first lead screw 22 is fixedly arranged at the output end of the first motor 21. A first sliding frame 23 is threadedly connected to the outer wall of the first lead screw 22. A slide bar 24 is slidably connected inside the first sliding frame 23. Both ends of the slide bar 24 are fixedly connected to the outer wall of the rotating frame 12. A cleaning brush 25 is fixedly connected to the outer wall of the first sliding frame 23. The inner wall of the cleaning brush 25 is slidably connected to the outer wall of the stirring rod 13.

[0033] Specifically, when cleaning is required, start the first motor 21 to drive the first lead screw 22 to rotate. The first lead screw 22 pushes the first sliding frame 23 to descend. The slide bar 24 supports the sliding of the first sliding frame 23. The slide bar 24 drives the cleaning brush 25 to slide down and sleeve on the outer wall of the stirring rod 13. Then, through the rotation of the stirring rod 13, the cleaning brush 25 rotates in contact with the inner wall of the evaporation kettle 3 to clean the evaporation kettle 3.

[0034] Please refer to the appendix Figure 2 and the appendix Figure 4 , the liquid discharging mechanism 15 includes a water tank 26. The water tank 26 is communicated with the rotating frame 12. The water tank 26 is fixed inside the housing 1. A spray head 27 is arranged on the outer wall of the rotating frame 12.

[0035] Specifically, the upper half of the rotating frame 12 is a tube body, that is, the rotating frame 12 can connect the nozzle 27 with the water tank 26. The water tank 26 stores the cleaning liquid. By adding a valve, the flow of the cleaning liquid can be controlled. By using a rotary joint, both the rotary joint and the valve are existing technologies to avoid movement interference. The cleaning liquid is sprayed out through the nozzle 27 to enhance the cleaning effect.

[0036] Please refer to the attached Figure 5 - attached Figure 6 , inside the housing 1, there is an electric slide rail 30. A pipette 31 is slidably connected inside the electric slide rail 30. A storage rack 32 is installed inside the housing 1. A storage tank 33 is provided inside the housing 1. A feeding pipe 34 is provided on the lower surface of the storage tank 33.

[0037] Specifically, the storage rack 32 is used to place raw materials and dissolution liquids. In cooperation with the electric slide rail 30 and the pipette 31, raw materials and dissolution liquids can be added to the evaporation kettle 3. Some pipettes 31 do not have a telescopic effect. To avoid movement interference, an electric telescopic rod can be added between the electric slide rail 30 and the pipette 31 to drive the pipette 31 to lift. The storage tank 33 is used to store the column chromatography reagent. A valve and a feeding pipe 34 are provided on the lower side of the storage tank 33 to facilitate adding the column chromatography reagent to the column chromatography cup 38.

[0038] Please refer to the attached Figure 6 , an electric push rod 35 is installed inside the housing 1. The output end of the electric push rod 35 is fixedly connected to an electric gripper 36. A fixing frame 37 is provided on the outer wall of the electric gripper 36. A column chromatography cup 38 is fixedly connected to the inner wall of the fixing frame 37.

[0039] Specifically, the housing 1 fixes the electric push rod 35. The electric push rod 35 can drive the electric gripper 36 to slide. The fixing frame 37 can be fixed through the electric gripper 36, thereby fixing the column chromatography cup 38. Similarly, the electric push rod 35 can drive the column chromatography cup 38 to move inside the housing 1. The movement of the column chromatography cup 38 can facilitate feeding and discharging.

[0040] Please refer to the attached Figure 6 , a support two 39 is fixedly connected to the outer wall of the fixing frame 37. A motor two 40 is fixedly connected to the outer wall of the support two 39. A lead screw two 41 is fixedly arranged at the output end of the motor two 40. A sliding frame two 42 is threadedly connected to the outer wall of the lead screw two 41. The outer wall of the sliding frame two 42 is slidably connected inside the support two 39. An installation ring 43 is threadedly connected to the outer wall of the sliding frame two 42. A filter element 44 is fixedly connected to the lower surface of the installation ring 43.

[0041] Specifically, the fixing frame 37 fixes the second support 39, and the second support 39 fixes the second motor 40. Starting the second motor 40 can drive the rotation of the second lead screw 41. The second lead screw 41 can push the second sliding frame 42 to move up and down, thereby driving the filter element 44 to move up and down, adjusting the height of the filter element 44, and also lifting the filter element 44 out of the inner part of the column passing cup 38. The filter element 44 can be disassembled and replaced by rotating the mounting ring 43.

[0042] A method for using an automatic column passing machine includes the following steps: The raw materials are placed on the storage rack 32. First, the raw materials are transferred to the upper evaporation kettle 3 through the pipette 31, and heated and evaporated through the heating component. At the same time, the first motor 4 is started, and the evaporation kettle 3 is pushed to rotate respectively by the bevel gear pair 6 and the bevel gear three 7 by using the transmission mechanism 10 and the belt mechanism 11, and the stirring rod 13 rotates in the opposite direction to the evaporation kettle 3. After evaporation, a dissolving solution is added, and then the stirring rod 13 is driven to rotate through the transmission mechanism 10 for stirring and dissolving. After dissolution, it is transferred to the column passing cup 38 for column passing. The column passing cup 38 is pushed to move by the electric push rod 35, and the storage tank 33 adds the reagent for column passing into the inner part of the column passing cup 38. The column passing cup 38 is moved close to the lower evaporation kettle 3 to start column passing. The solution after column passing flows into the lower evaporation kettle 3 and is evaporated to dryness again through the heating component, and is also assisted in evaporation by stirring through the first motor 4. After evaporation to dryness, the required sample is obtained. The cleaning brush 25 is sleeved on the outer wall of the stirring rod 13 through the cleaning mechanism 14. While starting the first motor 4, the cleaning liquid is added through the liquid outlet mechanism 15 to clean the evaporation kettle 3. The electric gripper 36 releases the fixing frame 37, and the column passing cup 38 and the second support 39 are removed for waste treatment.

[0043] Specifically, the solution on the upper side is transferred through the pipette 31. Starting the bevel gear one 5 can push the column passing cup 38 to slide. Subsequently, by opening the valve of the upper evaporation kettle 3, the dissolving solution inside the evaporation kettle 3 can flow into the column passing cup 38. Then, by raising the sliding of the rotating shaft one 8 and opening the valve of the storage tank 33 to add the reagent, a valve is also provided on the lower side of the column passing cup 38. The solution after column passing can flow into the inner part of the lower evaporation kettle 3 from the column passing cup 38. A valve is also provided on the bottom plate of the lower evaporation kettle 3, and the powder sample can be put into the lower sample box.

[0044] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.

Claims

1. An automatic column chromatography machine, comprising a housing (1), characterized in that, Inside the said housing (1), there is a fixedly connected mounting plate (2). Inside the mounting plate (2), there is a rotatably connected evaporation kettle (3). Inside the housing (1), there is a first motor (4). The output end of the first motor (4) is fixedly provided with a first bevel gear (5). The outer wall of the first bevel gear (5) is rotatably connected inside the housing (1). The tooth end of the first bevel gear (5) is meshed and connected with a second bevel gear (6) and a third bevel gear (7). The outer walls of the second bevel gear (6) and the third bevel gear (7) are both rotatably connected inside the housing (1). The outer wall of the third bevel gear (7) is fixedly connected with a first rotating shaft (8). The outer wall of the second bevel gear (6) is fixedly connected with a second rotating shaft (9). The second rotating shaft (9) is connected with a transmission mechanism (10). The transmission mechanism (10) is connected with the evaporation kettle (3). The outer wall of the first rotating shaft (8) is connected with a belt mechanism (11). The belt mechanism (11) is connected with a rotating frame (12). The outer wall of the rotating frame (12) is rotatably connected inside the housing (1). The outer wall of the rotating frame (12) is provided with a cleaning mechanism (14) and a liquid discharging mechanism (15). The outer wall of the rotating frame (12) is fixedly connected with a stirring rod (13). Inside the housing (1), there is a heating component.

2. The automatic column chromatography machine according to claim 1, wherein The said heating component includes a first bracket (28). The outer wall of the first bracket (28) is fixedly connected inside the housing (1). The outer wall of the first bracket (28) is fixedly connected with a heating frame (29).

3. An automatic column chromatography machine according to claim 1, wherein, The said transmission mechanism (10) includes a circular gear (16). The inside of the circular gear (16) is fixedly connected to the outer wall of the second rotating shaft (9). The tooth end of the circular gear (16) is meshed and connected with a toothed ring (17). The inside of the toothed ring (17) is fixedly connected to the outer wall of the evaporation kettle (3).

4. An automatic column chromatography machine according to claim 1, characterized in that, The said belt mechanism (11) includes a transmission belt (19). The outer wall of a first pulley (18) is fixedly connected to the outer wall of the first rotating shaft (8). The first pulley (18) is connected with a transmission belt (19). The transmission belt (19) is connected with a second pulley (20). The inside of the second pulley (20) is fixedly connected to the outer wall of the rotating frame (12).

5. The automatic column chromatography machine according to claim 1, characterized in that, The said cleaning mechanism (14) includes a first motor (21). The outer wall of the first motor (21) is fixedly connected to the outer wall of the rotating frame (12). The output end of the first motor (21) is fixedly provided with a first lead screw (22). The outer wall of the first lead screw (22) is threadedly connected with a first sliding frame (23). The inside of the first sliding frame (23) is slidably connected with a sliding rod (24). Both ends of the sliding rod (24) are fixedly connected to the outer wall of the rotating frame (12). The outer wall of the first sliding frame (23) is fixedly connected with a cleaning brush (25). The inner wall of the cleaning brush (25) is slidably connected to the outer wall of the stirring rod (13).

6. The automatic column chromatography machine according to claim 1, characterized in that, The said liquid discharging mechanism (15) includes a water tank (26). The water tank (26) is communicated with the rotating frame (12). The water tank (26) is fixed inside the housing (1). The outer wall of the rotating frame (12) is provided with a nozzle (27).

7. An automatic column chromatography machine according to claim 1, wherein, Inside the housing (1), an electric slide rail (30) is provided. A pipette gun (31) is slidably connected inside the electric slide rail (30). A storage rack (32) is installed inside the housing (1). A storage tank (33) is provided inside the housing (1). A feed pipe (34) is provided on the lower surface of the storage tank (33).

8. An automatic column chromatography machine according to claim 1, characterized in that, An electric push rod (35) is installed inside the housing (1). The output end of the electric push rod (35) is fixedly connected to an electric gripper (36). A fixing frame (37) is provided on the outer wall of the electric gripper (36). A through-column cup (38) is fixedly connected to the inner wall of the fixing frame (37).

9. The automatic column chromatography machine according to claim 8, characterized in that, A second support (39) is fixedly connected to the outer wall of the fixing frame (37). A second motor (40) is fixedly connected to the outer wall of the second support (39). A second lead screw (41) is fixedly provided at the output end of the second motor (40). A second sliding frame (42) is threadedly connected to the outer wall of the second lead screw (41). The outer wall of the second sliding frame (42) is slidably connected inside the second support (39). An installation ring (43) is threadedly connected to the outer wall of the second sliding frame (42). A filter element (44) is fixedly connected to the lower surface of the installation ring (43).

10. A method for using an automatic column chromatography machine according to any one of claims 1-9, characterized in that, It includes the following steps: The raw materials are placed on the storage rack (32). First, the raw materials are transferred to the upper evaporation kettle (3) by the pipette gun (31), and heated and evaporated by the heating component. At the same time, the first motor (4) is started, and through the second bevel gear (6) and the third bevel gear (7), the evaporation kettle (3) is pushed to rotate by the transmission mechanism (10) and the belt mechanism (11) respectively, and the stirring rod (13) rotates in the opposite direction to the evaporation kettle (3). After evaporation, a dissolving solution is added, and then the stirring rod (13) is driven to rotate by the transmission mechanism (10) for stirring and dissolving. After dissolution, it is transferred to the through-column cup (38) for column chromatography. The through-column cup (38) is pushed to move by the electric push rod (35), and the storage tank (33) adds the reagent for column chromatography into the through-column cup (38). The through-column cup (38) is moved close to the lower evaporation kettle (3) to start column chromatography. The solution after column chromatography flows into the lower evaporation kettle (3), and is evaporated to dryness again by the heating component. Similarly, the first motor (4) is used for stirring to assist in evaporation to dryness. After evaporation to dryness, the required sample is obtained. The cleaning brush (25) is sleeved on the outer wall of the stirring rod (13) by the cleaning mechanism (14). When the first motor (4) is started, the cleaning liquid is added through the liquid outlet mechanism (15) to clean the evaporation kettle (3). The electric gripper (36) releases the fixing frame (37), and the through-column cup (38) and the second support (39) are removed for waste treatment.

Images