Sea cucumber active peptide extraction device based on biological enzymolysis

Abstract

The invention discloses a sea cucumber active peptide extraction device based on biological enzymolysis in the field of active peptide extraction, which comprises an equipment body, a crushing assembly, a dilute salt solution supplementing assembly, a stirring assembly, a fixing frame and a limiting assembly, sea cucumbers with viscera and silt removed are poured into the pretreatment box, pretreated through the smashing assembly and the dilute salt solution supplementing assembly and then fed into the constant-temperature stirring box, stirring is conducted through the stirring assembly, it is ensured that biological enzymes efficiently catalyze protein degradation under the optimal reaction condition, the yield of sea cucumber bioactive peptides is increased, and the treatment effect is good. The mixed solution after enzymolysis enters the filter box, macromolecular impurities are removed through an ultrafiltration membrane technology, only target peptide fragments are reserved, then the extract is further concentrated through the purification and collection device, the purity and biological activity of the active peptide are improved, manual multi-procedure operation is not needed, and the extraction efficiency is improved.

Description

Technical Field

[0001] The present invention relates to the technical field of active peptide extraction, and particularly to a sea cucumber active peptide extraction device based on biological enzymolysis. Background Art

[0002] Sea cucumbers are precious marine organisms, rich in various bioactive components such as proteins, polysaccharides, saponins, lipids, and trace elements, and have various physiological functions such as antioxidant, anti-inflammatory, immunomodulatory, and anti-tumor effects. Among them, sea cucumber proteins can be enzymatically hydrolyzed or fermented to produce small molecular peptides (active peptides) with high biological activity, which have low molecular weight, high absorption efficiency, and stronger biological activity, and have broad application prospects in the fields of functional foods, pharmaceuticals, and cosmetics.

[0003] Traditional methods for extracting sea cucumber active peptides mainly include enzymolysis, microbial fermentation, chemical hydrolysis, and physical extraction methods (such as ultrasonic-assisted extraction, ultra-high pressure extraction, etc.). Enzymolysis is the most widely used technology at present. By selecting appropriate proteases (such as trypsin, alkaline protease, papain, etc.) to hydrolyze sea cucumber proteins under suitable conditions, target active peptides can be obtained efficiently.

[0004] However, most of the existing operations for extracting sea cucumber active peptides by biological enzymolysis require manual operations for multiple processes, which are cumbersome and have low extraction efficiency. Summary of the Invention

[0005] The purpose of this part is to outline some aspects of the embodiments of the present invention and briefly introduce some preferred embodiments. Some simplifications or omissions may be made in this part, as well as in the abstract and title of the present application, to avoid obscuring the purpose of this part, the abstract, and the title, but such simplifications or omissions shall not be used to limit the scope of the present invention.

[0006] In view of the above and / or problems existing in a sea cucumber active peptide extraction device based on biological enzymolysis, the present invention is proposed.

[0007] Therefore, the purpose of the present invention is to provide a sea cucumber active peptide extraction device based on biological enzymolysis. After removing the viscera and sediment, the sea cucumbers are poured into the internal part of the pretreatment tank and pretreated through the crushing component and the dilute salt solution supplement component, and then enter the internal part of the constant temperature stirring tank. The stirring component is used for stirring to ensure that the biological enzyme efficiently catalyzes the degradation of proteins under the optimal reaction conditions, improving the yield of sea cucumber active peptides. The mixed solution after enzymolysis enters the filtration tank, and macromolecular impurities are removed through ultrafiltration membrane technology, only retaining the target peptide segments. Subsequently, the purification and collection device further concentrates the extract, improving the purity and biological activity of the active peptides, without the need for manual operations for multiple processes, and improving the extraction efficiency.

[0008] To solve the above technical problems, according to one aspect of the present invention, the present invention provides the following technical solutions: A sea cucumber active peptide extraction device based on biological enzymolysis, comprising: A device body, including a pretreatment tank, a constant temperature stirring tank installed at the bottom of the pretreatment tank, a stirring tank installed at the bottom of the constant temperature stirring tank, and a collection and purification device installed at the bottom of the stirring tank; A crushing component, installed inside the pretreatment tank, for pretreating sea cucumbers and dissolving proteins; A dilute salt solution supplement component, installed on the side wall of the pretreatment tank and connected to the crushing component, for supplementing dilute salt solution inside the pretreatment tank; A stirring component, located inside the constant temperature stirring tank and connected to the crushing component, for accelerating the degradation of proteins inside the constant temperature stirring tank; A fixing frame, located inside the stirring tank, and a multi-layer ultrafiltration membrane is installed inside the fixing frame, for removing macromolecular impurities and retaining target peptide segments; A limiting component, installed on the side wall of the fixing frame, for limiting and fixing the fixing frame inside the stirring tank.

[0009] As a preferred scheme of the sea cucumber active peptide extraction device based on biological enzymolysis of the present invention, wherein, both sides of the inner bottom of the pretreatment tank have slopes, a discharge groove is opened at a position between the two slopes at the inner bottom of the pretreatment tank, and guiding grooves are opened on the symmetrical side walls of the pretreatment tank.

[0010] As a preferred scheme of the sea cucumber active peptide extraction device based on biological enzymolysis of the present invention, wherein, the crushing component includes a motor installed on the side wall of the pretreatment tank, a baffle located at the bottom of the pretreatment tank, and a shaft rod rotatably connected inside the pretreatment tank. A third threaded rod is installed at the output end of the motor, a first guiding rod is installed at the bottom of the pretreatment tank, a first threaded hole is opened on the side wall of the baffle, the third threaded rod rotates through the first threaded hole, a first guiding hole is also opened on the side wall of the baffle, the first guiding rod passes through the first guiding hole, two first guiding plates are installed on the symmetrical side walls of the baffle, the two first guiding plates respectively pass through the two guiding grooves, a second rack is installed at the top of one of the first guiding plates, a third rack is installed at the bottom of the other first guiding plate, a first gear is rotatably connected to the side wall of the pretreatment tank, the first gear meshes with the second rack, a first pulley is installed on the side wall of the first gear, a plurality of crushing blades are installed on the rod body of the shaft rod, one end of the shaft rod extends out of the side wall of the pretreatment tank and is installed with a fourth pulley, and the fourth pulley is connected to the first pulley through a belt.

[0011] As a preferred embodiment of the sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention, a fixing plate is installed on the side wall of the pretreatment tank. A first helical gear is rotatably connected to the side wall of the fixing plate, and a first threaded rod is rotatably connected to the other side wall of the fixing plate. The first threaded rod is coaxially and fixedly connected to the first helical gear. The other end of the shaft rod extends out of the side wall of the pretreatment tank and is provided with a turntable. A second helical gear is installed on the side wall of the turntable, and the second helical gear meshes with the first helical gear.

[0012] As a preferred embodiment of the sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention, the dilute salt solution replenishing assembly includes a fixed pipe installed on the side wall of the pretreatment tank and a piston located inside the fixed pipe. A first delivery pipe is installed on the side wall of the fixed pipe, and the other end of the first delivery pipe is connected to an external dilute salt solution tank. A first one-way valve is installed on the body of the first delivery pipe. A second delivery pipe is installed on the top of the fixed pipe, and the other end of the second delivery pipe extends into the pretreatment tank. A second one-way valve is installed on the body of the second delivery pipe. A fixed rod is installed on the side wall of the piston, and a second threaded hole is opened on the side end of the fixed rod. The first threaded rod rotates and extends into the second threaded hole.

[0013] As a preferred embodiment of the sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention, a discharge hole is opened at the bottom inside the constant temperature stirring tank. A second gear is rotatably connected to the side wall of the constant temperature stirring tank. A second pulley is installed on the side wall of the second gear. A third pulley is also rotatably connected to the side wall of the constant temperature stirring tank. The third pulley and the second pulley are connected by a belt. A second threaded rod is installed on the side wall of the third pulley, and the second threaded rod extends into the constant temperature stirring tank. A second guide rod is installed inside the constant temperature stirring tank, and two first racks are symmetrically installed on the inner wall of the constant temperature stirring tank.

[0014] As a preferred embodiment of the sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention, the stirring assembly includes a moving frame slidably connected inside the constant temperature stirring tank and two second shaft rods symmetrically and rotatably connected inside the moving frame. A third threaded hole is opened on the side wall of the moving frame, and the second threaded rod rotates and passes through the third threaded hole. A second guide hole is also opened on the side wall of the moving frame, and the second guide rod passes through the second guide hole. Stirring blades are installed on the body of the second shaft rod. One end of the second shaft rod extends out of the side wall of the moving frame and is provided with a third gear, and the two first racks respectively mesh with the two third gears.

[0015] As a preferred embodiment of the sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention, an opening is provided on the side wall of the stirring tank, a second guiding groove is provided at a position corresponding to the opening on the inner wall of the stirring tank, a limiting plate is installed on the side wall of the stirring tank, and a limiting hole is installed at the top of the limiting plate.

[0016] As a preferred embodiment of the sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention, the fixing frame is located inside the stirring tank, a cover plate is installed on the side wall of the fixing frame, the cover plate is located inside the opening, second guiding plates are symmetrically installed on the side walls of the fixing frame, and the second guiding plates are located inside the second guiding grooves.

[0017] As a preferred embodiment of the sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention, the limiting component includes a housing installed on the side wall of the stirring tank, a sliding plate slidably connected inside the housing, a spring installed at the bottom of the sliding plate, and a limiting rod installed at the top of the sliding plate. A pulling plate is installed on the side wall of the sliding plate, and the top end of the limiting rod extends out of the side wall of the housing.

[0018] Compared with the prior art: by arranging a crushing component and a dilute salt solution supplementing component inside the pretreatment tank, arranging a constant temperature stirring tank at the bottom of the pretreatment tank, with a stirring component arranged inside it, arranging a filtering tank at the bottom of the constant temperature stirring tank, with a fixing frame arranged inside it, and multiple ultrafiltration membranes arranged inside the fixing frame, arranging a collection and purification device at the bottom of the filtering tank, pouring the sea cucumber after removing internal organs and sediment into the pretreatment tank for pretreatment through the crushing component and the dilute salt solution supplementing component and then entering the constant temperature stirring tank, stirring through the stirring component to ensure that the biological enzyme efficiently catalyzes protein degradation under the optimal reaction conditions, improving the yield of sea cucumber active peptides. The enzymolyzed mixed solution enters the filtering tank, and macromolecular impurities are removed through the ultrafiltration membrane technology, only retaining the target peptide segments. Subsequently, the purification and collection device further concentrates the extract, improving the purity and biological activity of the active peptides, without the need for manual multi-process operations, and improving the extraction efficiency. Description of the Drawings

[0019] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the present invention will be described in detail below in conjunction with the drawings and detailed embodiments. Obviously, the drawings in the following description are only some embodiments of the present invention. For those of ordinary skill in the art, other drawings can be obtained based on these drawings without creative efforts. Among them: Figure 1 It is the overall structure diagram of a sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention; Figure 2Structural diagram of the bottom of the pretreatment tank of a sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention; Figure 3 Internal structural diagram of the pretreatment tank of a sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention; Figure 4 Structural diagram of the dilute salt solution supplement component of a sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention; Figure 5 Internal structural diagram of the constant temperature stirring tank of a sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention; Figure 6 Structural diagram of the stirring component of a sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention; Figure 7 Structural diagram of the filtration tank of a sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention; Figure 8 Structural diagram of the limiting component of a sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention. Detailed implementation manners

[0020] To make the above objects, features and advantages of the present invention more obvious and understandable, the following will describe the detailed implementation manners of the present invention with reference to the accompanying drawings.

[0021] Secondly, the present invention will be described in detail with reference to the schematic diagrams. When describing the embodiments of the present invention in detail, for the convenience of description, the cross-sectional views showing the device structure will be enlarged locally out of the general proportion, and the schematic diagrams are only examples and should not limit the scope of protection of the present invention herein. In addition, in actual production, three-dimensional spatial dimensions including length, width and depth should be included.

[0022] To make the purpose, technical solution and advantages of the present invention clearer, the following will further describe the embodiments of the present invention in detail with reference to the accompanying drawings.

[0023] The present invention provides a sea cucumber active peptide extraction device based on biological enzymolysis. After removing the internal organs and sediment, the sea cucumbers are poured into the interior of the pretreatment tank and pretreated through a crushing component and a dilute salt solution supplement component, and then enter the interior of the constant temperature stirring tank. Stirring is carried out through the stirring component to ensure that the biological enzyme efficiently catalyzes the degradation of proteins under the optimal reaction conditions, improving the yield of sea cucumber active peptides. The mixed solution after enzymolysis enters the filtration tank, and macromolecular impurities are removed through ultrafiltration membrane technology, only retaining the target peptide segments. Subsequently, the purification and collection device further concentrates the extract, improving the purity and biological activity of the active peptides, and eliminating the need for manual multi-process operations, thus improving the extraction efficiency.

[0024] Figure 1-8 Shown is a structural schematic diagram of an embodiment of a sea cucumber active peptide extraction device based on biological enzymolysis according to the present invention. Please refer toFigure 1 - Figure 8 , an extraction device for sea cucumber active peptides based on biological enzymolysis in this embodiment includes a device body 100, a crushing component 200, a dilute salt solution supplementing component 300, a stirring component 400, a fixing frame 500 and a limiting component 600.

[0025] The device body 100 includes a pretreatment tank 110, a constant temperature stirring tank 120 installed at the bottom of the pretreatment tank 110, a stirring tank 130 installed at the bottom of the constant temperature stirring tank 120, and a collection and purification device 140 installed at the bottom of the stirring tank 130. There are slopes 110a-1 on both sides of the inner bottom of the pretreatment tank 110. A discharge groove 110a is formed at the position between the two slopes 110a-1 on the inner bottom of the pretreatment tank 110. Guide grooves 110a-2 are formed on the symmetrical side walls of the pretreatment tank 110. The pretreated sea cucumbers are located inside the pretreatment tank 110, and will slide along the slopes 110a-1 towards the guide grooves 110a-2, and fall into the constant temperature stirring tank 120 from the guide grooves 110a-2.

[0026] The crushing assembly 200 is installed inside the pretreatment tank 110 and is used for pretreating sea cucumbers and dissolving proteins. The crushing assembly 200 includes a motor 210 installed on the side wall of the pretreatment tank 110, a baffle 220 located at the bottom of the pretreatment tank 110, and a shaft rod 230 rotatably connected inside the pretreatment tank 110. A third threaded rod 210a is installed at the output end of the motor 210. A first guide rod 110b is installed at the bottom of the pretreatment tank 110. A first threaded hole 220a is formed in the side wall of the baffle 220. The third threaded rod 210a rotatably penetrates through the first threaded hole 220a. A first guide hole 220c is also formed in the side wall of the baffle 220. The first guide rod 110b penetrates through the first guide hole 220c. Two first guide plates 220b are installed on the symmetrical side walls of the baffle 220. The two first guide plates 220b respectively penetrate through two guide grooves 110a-2. A second rack 220b-1 is installed at the top of one of the first guide plates 220b, and a third rack 220b-2 is installed at the bottom of the other first guide plate 220b. A first gear 110c is rotatably connected to the side wall of the pretreatment tank 110. The first gear 110c meshes with the second rack 220b-1. A first pulley 110c-1 is installed on the side wall of the first gear 110c. A plurality of crushing blades 230a are installed on the rod body of the shaft rod 230. One end of the shaft rod 230 extends out of the side wall of the pretreatment tank 110 and is installed with a fourth pulley 230b. The fourth pulley 230b is connected to the first pulley 110c-1 through a belt. A fixing plate 110d is installed on the side wall of the pretreatment tank 110. A first bevel gear 110d-1 is rotatably connected to the side wall of the fixing plate 110d. A first threaded rod 110d-2 is rotatably connected to the other side wall of the fixing plate 110d. The first threaded rod 110d-2 is coaxially and fixedly connected to the first bevel gear 110d-1. The other end of the shaft rod 230 extends out of the side wall of the pretreatment tank 110 and is installed with a turntable 230c. A second bevel gear 230c-1 is installed on the side wall of the turntable 230c. The second bevel gear 230c-1 meshes with the first bevel gear 110d-1. In the initial state, the shaft rod 230 is located on one side of the guide groove 110a-2 and does not block the guide groove 110a-2. By starting the motor 210 to drive the third threaded rod 210a to rotate, when the third threaded rod 210a rotates, it uses the screw structure to push the baffle 220 to drive the first guide plate 220b to move left and right. When the baffle 220 moves, it passes through the guide groove 110a-2 and blocks the guide groove 110a-2. When the baffle 220 resets, the guide groove 110a-2 is exposed. The pretreated solution inside the pretreatment tank 110 falls into the constant temperature stirring tank 120 through the guide groove 110a-2. When the first guide plate 220b moves, it drives the second rack 220b-1 to move. The second rack 220b-1 drives the first gear 110c and the first pulley 110c-1 to rotate. When the first pulley 110c-1 rotates, it uses the belt to drive the fourth pulley 230b and the shaft rod 230 to rotate, and then drives the crushing blades 230a to rotate.Pulverize the sea cucumbers inside the discharge chute 110a.

[0027] The dilute salt solution replenishing component 300 is installed on the side wall of the pretreatment tank 110 and is connected to the pulverizing component 200 for replenishing the dilute salt solution inside the pretreatment tank 110. The dilute salt solution replenishing component 300 includes a fixed pipe 310 installed on the side wall of the pretreatment tank 110 and a piston 320 located inside the fixed pipe 310. A first delivery pipe 310a is installed on the side wall of the fixed pipe 310, and the other end of the first delivery pipe 310a is connected to an external dilute salt solution tank. A first one-way valve 310a-1 is installed on the body of the first delivery pipe 310a. A second delivery pipe 310b is installed on the top of the fixed pipe 310, and the other end of the second delivery pipe 310b extends into the pretreatment tank 110. A second one-way valve 310b-1 is installed on the body of the second delivery pipe 310b. A fixed rod 320a is installed on the side wall of the piston 320, and a second threaded hole 320a-1 is formed in the side end of the fixed rod 320a. The first threaded rod 110d-2 rotates and extends into the second threaded hole 320a-1. When the shaft rod 230 rotates, it drives the turntable 230c and the second helical gear 230c-1 to rotate. When the second helical gear 230c-1 rotates, it drives the first helical gear 110d-1 and the first threaded rod 110d-2 to rotate. When the first threaded rod 110d-2 rotates, it uses the screw rod structure to push the fixed rod 320a and the piston 320 to reciprocate inside the fixed pipe 310. When the piston 320 moves towards the outside of the fixed pipe 310, at this time the first one-way valve 310a-1 opens and the second one-way valve 310b-1 closes, and the external dilute salt solution enters the fixed pipe 310 through the first delivery pipe 310a. When the piston 320 moves towards the inside of the fixed pipe 310, at this time the first one-way valve 310a-1 closes and the second one-way valve 310b-1 opens, and the dilute salt solution inside the fixed pipe 310 enters the discharge chute 110a through the second delivery pipe 310b.

[0028] The stirring assembly 400 is located inside the constant-temperature stirring tank 120 and is connected to the crushing assembly 200, and is used to accelerate the protein degradation inside the constant-temperature stirring tank 120. A discharge hole 120a is provided at the inner bottom of the constant-temperature stirring tank 120. A second gear 120b is rotatably connected to the side wall of the constant-temperature stirring tank 120, and a second pulley 120b-1 is installed on the side wall of the second gear 120b. A third pulley 120c is also rotatably connected to the side wall of the constant-temperature stirring tank 120. The third pulley 120c is connected to the second pulley 120b-1 by a belt. A second threaded rod 120c-1 is installed on the side wall of the third pulley 120c. The second threaded rod 120c-1 extends into the constant-temperature stirring tank 120. A second guide rod 120c-2 is installed inside the constant-temperature stirring tank 120. Two first racks 120d are symmetrically installed on the inner wall of the constant-temperature stirring tank 120. The stirring assembly 400 includes a moving frame 410 slidably connected inside the constant-temperature stirring tank 120 and two second shaft rods 420 symmetrically rotatably connected inside the moving frame 410. A third threaded hole 410a is provided on the side wall of the moving frame 410. The second threaded rod 120c-1 rotates through the third threaded hole 410a. A second guide hole 410b is also provided on the side wall of the moving frame 410. The second guide rod 120c-2 passes through the second guide hole 410b. A stirring blade 420a is installed on the rod body of the second shaft rod 420. One end of the second shaft rod 420 extends out of the side wall of the moving frame 410 and a third gear 420b is installed. The two first racks 120d are respectively engaged with the two third gears 420b. When the first guide plate 220b moves, it drives the third rack 220b-2 to move. The third rack 220b-2 drives the second gear 120b and the second pulley 120b-1 to rotate. When the second pulley 120b-1 rotates, it drives the third pulley 120c and the second threaded rod 120c-1 to rotate by using the belt. When the second threaded rod 120c-1 rotates, it uses the screw structure to push the moving frame 410 to move inside the constant-temperature stirring tank 120. When the moving frame 410 moves, the first rack 120d drives the third gear 420b to rotate, and then drives the second shaft rod 420 and the stirring blade 420a to rotate, so as to stir the solution inside the constant-temperature stirring tank 120.

[0029] The fixed frame 500 is located inside the stirring tank 130. Inside the fixed frame 500, multiple ultrafiltration membranes 510 are installed, which are used to remove macromolecular impurities and retain the target peptide segments. The limiting component 600 is installed on the side wall of the fixed frame 500 and is used to limit and fix the fixed frame 500 inside the stirring tank 130. An opening 130a is provided on the side wall of the stirring tank 130, and a second guiding groove 130a-1 is provided at the corresponding position on the inner wall of the stirring tank 130. A limiting plate 130b is installed on the side wall of the stirring tank 130, and a limiting hole 130b-1 is installed at the top of the limiting plate 130b. The fixed frame 500 is located inside the stirring tank 130, and a cover plate 520 is installed on the side wall of the fixed frame 500. The cover plate 520 is located inside the opening 130a. Second guiding plates 530 are installed on the symmetrical side walls of the fixed frame 500, and the second guiding plates 530 are located inside the second guiding groove 130a-1. The limiting component 600 includes a housing 610 installed on the side wall of the stirring tank 130, a sliding plate 620 slidably connected inside the housing 610, a spring 630 installed at the bottom of the sliding plate 620, and a limiting rod 640 installed at the top of the sliding plate 620. A pulling plate 620a is installed on the side wall of the sliding plate 620, and the top end of the limiting rod 640 extends out of the side wall of the housing 610. The stirred solution enters the inside of the stirring tank 130 through the discharge hole 120a. When the solution passes through the multiple ultrafiltration membranes 510, the multiple ultrafiltration membranes 510 filter out the macromolecular impurities in the solution and only retain the target peptide segments. When it is necessary to replace the multiple ultrafiltration membranes 510, pull the sliding plate 620 to move inside the housing 610 and compress the spring 630. The limiting rod 640 slides with the sliding plate 620 and retracts into the housing 610. The limiting rod 640 separates from the inside of the limiting hole 130b-1. At this time, pull the cover plate 520 to drive the fixed frame 500 and the multiple ultrafiltration membranes 510 to be drawn out from the opening of the opening 130a. After replacing the multiple ultrafiltration membranes 510, insert the fixed frame 500 into the inside of the stirring tank 130 through the opening 130a. The second guiding plates 530 slide inside the second guiding groove 130a-1 until the side wall of the fixed frame 500 abuts against the inner wall of the stirring tank 130. The cover plate 520 shields the opening of the opening 130a. Release the sliding plate 620, and the spring 630 rebounds to push the sliding plate 620 and the limiting rod 640 to move upward. The limiting rod 640 passes through the limiting hole 130b-1 to limit and fix the fixed frame 500 and the multiple ultrafiltration membranes 510 inside the stirring tank 130.

[0030] Combine Figure 1 - Figure 8, A sea cucumber active peptide extraction device based on biological enzymolysis in this embodiment. When in use, first clean the sea cucumber, remove the internal organs and sediment of the sea cucumber and cut it into sections. Then pour the cut sea cucumbers into the interior of the pretreatment box 110. Start the motor 210 to drive the rotation of the third threaded rod 210a, and push the baffle 220 to reciprocate at the bottom of the pretreatment box 110. When the baffle 220 moves, it drives the rotation of the first gear 110c through the second rack 220b-1, and then drives the rotation of the shaft rod 230 and the crushing blade 230a to crush the sea cucumber sections inside the pretreatment box 110. During the crushing process, the shaft rod 230 drives the rotation of the first threaded rod 110d-2 through the turntable 230c, and pushes the piston 320 to reciprocate inside the fixed tube 310 to inject dilute salt solution into the interior of the pretreatment box 110. And every time the baffle 220 resets, the solution falls into the interior of the constant temperature stirring box 120 through the discharge slot 110a. And when the third rack 220b-2 moves, it drives the rotation of the second gear 120b, and then drives the rotation of the second threaded rod 120c-1 to push the moving frame 410 to move inside the constant temperature stirring box 120. When the moving frame 410 moves, the first rack 120d drives the rotation of the third gear 420b and the second shaft rod 420, and the second shaft rod 420 drives the rotation of the stirring blade 420a to stir the solution inside the constant temperature stirring box 120, ensuring that the biological enzyme efficiently catalyzes the degradation of proteins under the optimal reaction conditions, improving the yield of sea cucumber active peptides. The enzymolyzed mixture enters the interior of the stirring box 130 and passes through multiple ultrafiltration membranes 510. The macromolecular impurities are removed through the ultrafiltration membrane technology, and only the target peptide segments are retained. Subsequently, the collection and purification device 140 further concentrates the extract to improve the purity and biological activity of the active peptides.

[0031] Although the present invention has been described above with reference to the embodiments, various improvements can be made to it and components therein can be replaced with equivalents without departing from the scope of the present invention. In particular, as long as there is no structural conflict, the various features in the embodiments disclosed in the present invention can be combined with each other in any way. The reason for not exhaustively describing the situations of these combinations in this specification is only to save space and resources. Therefore, the present invention is not limited to the specific embodiments disclosed in the text, but includes all technical solutions falling within the scope of the claims.

Claims

1. An extraction device for sea cucumber active peptides based on biological enzymatic hydrolysis, characterized in that, Comprising: The device body (100) includes a pretreatment box (110), a constant temperature stirring box (120) installed at the bottom of the pretreatment box (110), a stirring box (130) installed at the bottom of the constant temperature stirring box (120), and a collection and purification device (140) installed at the bottom of the stirring box (130); The crushing component (200) is installed inside the pretreatment box (110) and is used for pretreating sea cucumbers and dissolving proteins; The dilute salt solution supplementing component (300) is installed on the side wall of the pretreatment box (110) and is connected to the crushing component (200) for supplementing dilute salt solution inside the pretreatment box (110); The stirring component (400) is located inside the constant temperature stirring box (120) and is connected to the crushing component (200) for accelerating the degradation of proteins inside the constant temperature stirring box (120); The fixing frame (500) is located inside the stirring box (130), and a multi-layer ultrafiltration membrane (510) is installed inside the fixing frame (500) for removing macromolecular impurities and retaining target peptide segments; The limiting component (600) is installed on the side wall of the fixing frame (500) for limiting and fixing the fixing frame (500) inside the stirring box (130).

2. The extraction device for sea cucumber active peptides based on biological enzymolysis according to claim 1, wherein Both sides of the inner bottom of the pretreatment box (110) have slopes (110a-1), a discharge groove (110a) is formed at the position between the two slopes (110a-1) on the inner bottom of the pretreatment box (110), and guide grooves (110a-2) are formed on the symmetric side walls of the pretreatment box (110).

3. The extraction device of sea cucumber active peptides based on biological enzymolysis according to claim 2, characterized in that, The crushing assembly (200) includes a motor (210) installed on the side wall of the pretreatment tank (110), a baffle (220) located at the bottom of the pretreatment tank (110), and a shaft rod (230) rotatably connected inside the pretreatment tank (110). A third threaded rod (210a) is installed at the output end of the motor (210). A first guide rod (110b) is installed at the bottom of the pretreatment tank (110). A first threaded hole (220a) is formed in the side wall of the baffle (220). The third threaded rod (210a) rotatably penetrates through the first threaded hole (220a). A first guide hole (220c) is also formed in the side wall of the baffle (220). The first guide rod (110b) penetrates through the first guide hole (220c). Two first guide plates (220b) are installed on the symmetrical side walls of the baffle (220). The two first guide plates (220b) respectively penetrate through the two guide grooves (110a-2). A second rack (220b-1) is installed at the top of one of the first guide plates (220b), and a third rack (220b-2) is installed at the bottom of the other first guide plate (220b). A first gear (110c) is rotatably connected to the side wall of the pretreatment tank (110). The first gear (110c) meshes with the second rack (220b-1). A first pulley (110c-1) is installed on the side wall of the first gear (110c). A plurality of crushing blades (230a) are installed on the rod body of the shaft rod (230). One end of the shaft rod (230) extends out of the side wall of the pretreatment tank (110) and is installed with a fourth pulley (230b). The fourth pulley (230b) is connected to the first pulley (110c-1) by a belt.

4. An extraction device for sea cucumber active peptides based on biological enzymolysis according to claim 3, characterized in that, A fixing plate (110d) is installed on the side wall of the pretreatment tank (110). A first helical gear (110d-1) is rotatably connected to the side wall of the fixing plate (110d). A first threaded rod (110d-2) is rotatably connected to the other side wall of the fixing plate (110d). The first threaded rod (110d-2) is coaxially and fixedly connected to the first helical gear (110d-1). The other end of the shaft rod (230) extends out of the side wall of the pretreatment tank (110) and is installed with a turntable (230c). A second helical gear (230c-1) is installed on the side wall of the turntable (230c). The second helical gear (230c-1) meshes with the first helical gear (110d-1).

5. The extraction device of sea cucumber active peptides based on biological enzymolysis according to claim 4, characterized in that The dilute salt solution replenishing component (300) includes a fixed pipe (310) installed on the side wall of the pretreatment tank (110) and a piston (320) located inside the fixed pipe (310). A first delivery pipe (310a) is installed on the side wall of the fixed pipe (310), and the other end of the first delivery pipe (310a) is connected to an external dilute salt solution tank. A first one-way valve (310a-1) is installed on the pipe body of the first delivery pipe (310a). A second delivery pipe (310b) is installed at the top of the fixed pipe (310), and the other end of the second delivery pipe (310b) extends into the pretreatment tank (110). A second one-way valve (310b-1) is installed on the pipe body of the second delivery pipe (310b). A fixed rod (320a) is installed on the side wall of the piston (320), and a second threaded hole (320a-1) is formed at the side end of the fixed rod (320a). The first threaded rod (110d-2) rotates and extends into the second threaded hole (320a-1).

6. The extraction device for sea cucumber active peptides based on biological enzymolysis according to claim 1, characterized in that, An outlet hole (120a) is formed at the inner bottom of the constant temperature stirring tank (120). A second gear (120b) is rotatably connected to the side wall of the constant temperature stirring tank (120). A second pulley (120b-1) is installed on the side wall of the second gear (120b). A third pulley (120c) is also rotatably connected to the side wall of the constant temperature stirring tank (120). The third pulley (120c) is connected to the second pulley (120b-1) by a belt. A second threaded rod (120c-1) is installed on the side wall of the third pulley (120c). The second threaded rod (120c-1) extends into the constant temperature stirring tank (120). A second guide rod (120c-2) is installed inside the constant temperature stirring tank (120). Two first racks (120d) are symmetrically installed on the inner wall of the constant temperature stirring tank (120).

7. The extraction device for sea cucumber active peptides based on biological enzymolysis according to claim 6, wherein, The stirring component (400) includes a moving frame (410) slidably connected inside the constant temperature stirring tank (120) and two second shaft rods (420) symmetrically and rotatably connected inside the moving frame (410). A third threaded hole (410a) is formed on the side wall of the moving frame (410). The second threaded rod (120c-1) rotates and passes through the third threaded hole (410a). A second guide hole (410b) is also formed on the side wall of the moving frame (410). The second guide rod (120c-2) passes through the second guide hole (410b). Stirring blades (420a) are installed on the rod bodies of the second shaft rods (420). One end of each second shaft rod (420) extends out of the side wall of the moving frame (410) and is installed with a third gear (420b). The two first racks (120d) are respectively engaged with the two third gears (420b).

8. The extraction device of sea cucumber active peptides based on biological enzymolysis according to claim 1, characterized in that, The side wall of the mixing tank (130) is provided with an opening (130a), a second guiding groove (130a-1) is provided at a position corresponding to the opening (130a) on the inner wall of the mixing tank (130), a limiting plate (130b) is installed on the side wall of the mixing tank (130), and a limiting hole (130b-1) is installed at the top of the limiting plate (130b).

9. A sea cucumber active peptide extraction device based on biological enzymolysis according to claim 8, characterized in that, The fixing frame (500) is located inside the mixing tank (130), a cover plate (520) is installed on the side wall of the fixing frame (500), the cover plate (520) is located inside the opening (130a), second guiding plates (530) are symmetrically installed on the side walls of the fixing frame (500), and the second guiding plates (530) are located inside the second guiding groove (130a-1).

10. A sea cucumber active peptide extraction device based on biological enzymolysis according to claim 1, characterized in that, The limiting component (600) includes a housing (610) installed on the side wall of the mixing tank (130), a sliding plate (620) slidably connected inside the housing (610), a spring (630) installed at the bottom of the sliding plate (620), and a limiting rod (640) installed at the top of the sliding plate (620). A pulling plate (620a) is installed on the side wall of the sliding plate (620), and the top end of the limiting rod (640) extends out of the side wall of the housing (610).

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