Cell separation and purification device and operation method thereof

Abstract

The invention discloses a cell separation and purification device and an operation method thereof, and relates to the technical field of medical instruments. The cell separation and purification device comprises an operation table and an incubator mounted on the operation table, a telescopic rod, an ice groove, a mechanical arm, a slidable annular groove and a slidable annular magnet are further installed on the operation table. A smashing mechanism is connected to the telescopic rod, and a liquid storage unit used for synchronously adding a buffer solution is further arranged in the smashing mechanism. Test operation can be carried out outside the incubator, interference of bacteria on purification possibly caused by direct manual experiment is avoided, and improvement of cell activity is facilitated; a liquid storage pipe and a rotating shaft are arranged in the crushing mechanism, so that a buffer solution can be synchronously added in the crushing process, and the cell activity can be improved while crushing is accelerated; a screening plate of the crushing mechanism is in transmission connection with the rotating shaft, so that the screening plate moves upwards in the crushing process, and screening is accelerated; and an anti-fouling mechanism is arranged to prevent the operation table from being polluted after the crushing mechanism is taken out.

Description

Technical Field

[0001] The present invention relates to the technical field of medical devices, and in particular to a cell separation and purification device and an operating method thereof. Background Art

[0002] With the in-depth development of life science research, cell separation and purification technology plays a vital role in medical diagnosis, treatment, and biological research. For example, the separation and purification of glial cells often uses different biological characteristics of different glial cells and different markers, and uses magnetic beads with different markers to sort glial cells. Taking the extraction of primary microglia as an example, the magnetic bead sorting method for cell purification generally includes the following steps: 1. Sampling: Select the required part for anatomical separation, and after separation, cut the tissue into tissue particles of size 1*1*1mm (this step is performed on ice); 2. Digestion: Add an appropriate amount of trypsin to the shredded tissue particles, put it in a 5% carbon dioxide incubator at 37°C, shake the culture dish every 5 minutes, mix the cells to fully digest, and filter to obtain a cell suspension; 3. Purify the inoculated cells: Mix the cell suspension with specific magnetic beads, incubate for a certain period of time to allow the magnetic beads to bind to the target cells, wash and rinse the sorted cells with buffer, and collect them in a sterile container. However, the above purification operation is generally manual, the process is cumbersome, and the efficiency is low.

[0003] In response to the above problems, patent number CN108865879A discloses a cell separation and purification device, which includes a cutting module, a centrifugal module spaced apart from the cutting module, a transport module disposed between the cutting module and the centrifugal module, and a control unit. The cutting module includes a container defining a containing space and a tool mechanism for cutting the sample. However, the device adds a buffer after the cutting is completed, and during the cutting process of the tissue, the activity of the cells may decrease due to the decrease in wetness, which affects the normal purification operation. Summary of the invention

[0004] The present invention provides a cell separation and purification device and an operation method thereof, aiming to solve the problem that the existing cell separation and purification device cannot automatically add buffer solution during tissue cutting, which may affect cell activity.

[0005] In order to achieve the above-mentioned purpose, the technical ideas adopted by the present invention to solve the technical problems are as follows: A cell separation and purification device and an operating method thereof are designed. A liquid storage tube and a knife head are installed on a rotating shaft, and a piston head connected to the rotating shaft is arranged in the liquid storage tube. When the rotating shaft rotates, the knife head rotates to cut and crush the tissue sample, and the piston head moves downward with the rotation of the rotating shaft to add buffer solution into the test tube to maintain the activity of the cells.

[0006] To achieve the above object, the technical solution adopted by the present invention to solve its technical problems is as follows: A cell separation and purification device, comprising an operation table and an incubator installed on the operation table; An expansion rod, an ice trough, a robotic arm, as well as a slidable annular groove and an annular magnet are further installed on the operation table; The ice trough, the annular groove and the annular magnet are used for placing test tubes; A crushing mechanism is connected to the expansion rod, and a liquid storage unit for synchronously adding a buffer solution is further provided in the crushing mechanism.

[0007] Further, the crushing mechanism includes a motor, a rotating shaft and a cutter head; The motor is connected to the expansion rod through a connecting plate; The rotating shaft is connected to the output shaft of the motor; The cutter head is provided on the rotating shaft.

[0008] Further, the liquid storage unit includes a limiting rod and a liquid storage pipe installed on the connecting plate; The rotating shaft penetrates through the liquid storage pipe; A plurality of through holes are provided on the bottom plate of the liquid storage pipe; A piston head is hermetically and slidably arranged in the liquid storage pipe, and the piston head is sleeved on the rotating shaft and the limiting rod.

[0009] Further, the crushing mechanism further includes a screening plate; The screening plate is sleeved on the bottom end of the rotating shaft, and the limiting rod penetrates through the screening plate.

[0010] Further, an anti-pollution mechanism is further provided between the expansion rod and the crushing mechanism, and the anti-pollution mechanism is used to prevent liquid from flowing out and polluting the operation table when the crushing mechanism is taken out.

[0011] Further, the expansion rod includes an inner tube and an outer tube movably sleeved outside the lower part of the inner tube; The inner tube is connected to the connecting plate; The outer tube is installed on the operation table.

[0012] An electric push rod is installed in the outer tube; A first limiting ring is provided at the bottom of the inner tube; A flexible limiting plate is provided at the top of the electric push rod; The limiting plate is matched with the first limiting ring; A third limiting ring matched with the limiting plate is provided in the inner tube; The limiting plate is connected to the anti-pollution mechanism.

[0013] Further, the anti-fouling mechanism includes a rope, a spring and an umbrella assembly; One end of the rope is connected to the limit plate, and the other end passes through the connecting plate and the motor and is connected to the umbrella assembly; The spring sleeve is arranged outside the rope, and the upper end is installed on the motor, and the lower end is connected to the umbrella assembly; The spring and umbrella assembly are sleeved in the rotating shaft.

[0014] Furthermore, the operation method of the cell separation and purification device comprises the following steps: S1: Place the test tube on an ice tank, start the crushing mechanism to crush the tissue sample, and add buffer into the test tube while crushing; S2: Start the telescopic rod to move the crushing mechanism out of the test tube; S3: operate the robotic arm to place the test tube into the annular groove, move the annular groove into the incubator, complete the digestion and filtration of the cells, and then move the test tube out of the incubator; S4: Operate the robotic arm to place the test tube into the ring magnet, move the ring magnet into the incubator, and complete the purification.

[0015] The beneficial effects of the present invention are as follows: compared with the prior art, the present invention has the following improvements: 1. The present invention optimizes the cell separation and purification steps that originally needed to be performed manually in the incubator to be controlled and operated outside the incubator by setting an operating table, a mechanical arm and an incubator, which not only avoids the interference of bacteria on the purification steps that may be caused by manual direct experiments, but also helps to maintain a constant temperature and carbon dioxide content in the operating box, which can improve cell activity and facilitate the successful implementation; 2. The present invention provides a liquid storage tube and a rotating shaft in the pulverizing mechanism, so that a buffer solution can be automatically added during the pulverizing process. The buffer solution flushes the cutter head and the sieve plate, which can not only speed up the pulverizing process and improve the utilization rate of tissue samples, but also help to improve the wetness and activity of cells; 3. The present invention connects the screening plate of the crushing mechanism with the rotating shaft so that the screening plate can move upward during the crushing process, which helps to speed up the screening operation and improve the test efficiency; 4. The present invention provides an anti-fouling mechanism and connects the rope to the telescopic rod, so that when the inner tube moves upward, the crushing mechanism is first driven to be taken out, and then the umbrella assembly is opened, which can avoid contamination of the operating table after the crushing mechanism is taken out. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for use in the embodiments are briefly introduced below.

[0017] Figure 1It is a schematic diagram of the overall structure when the tissue sample is crushed by the present invention; Figure 2 It is Figure 1 the schematic diagram of the structure after the incubator in Figure 3 is opened; It is a schematic diagram of the structure when the trypsin reagent is added to the present invention; Figure 4 It is a schematic diagram of the structure when the filtrate is obtained by filtration of the present invention; Figure 5 It is a schematic diagram of the overall structure of the crushing mechanism; Figure 6 It is a schematic diagram of the structure of the liquid storage tube of the crushing mechanism after removing the side wall; Figure 7 It is a three-dimensional sectional view of the crushing mechanism; Figure 8 It is a schematic diagram of the anti-pollution mechanism; Figure 9 It is Figure 8 the A-A sectional view of Figure 10 It is Figure 9 the schematic diagram of the anti-pollution mechanism when the umbrella assembly in Figure 11 is opened; It is a three-dimensional sectional view of the anti-pollution mechanism and the crushing mechanism; In the figure: 1. Operating table; 11. Slide groove; 2. Telescopic rod; 21. Outer tube; 22. Inner tube; 23. Connecting plate; 24. First limiting ring; 25. Second limiting ring; 26. Electric push rod; 27. Limiting plate; 28. Third limiting ring; 3. Ice trough; 4. Test tube; 5. Crushing mechanism; 51. Motor; 52. Rotating shaft; 521. First thread section; 522. Second thread section; 53. Limiting rod; 54. Knife head; 55. Liquid storage tube; 551. Piston head; 552. Bottom plate; 553. Through hole; 56. Screening plate; 6. Anti-pollution mechanism; 61. Rope; 62. Spring; 63. Umbrella assembly; 7. Annular groove; 71. Moving plate; 8. Manipulator; 9. Incubator; 91. Magnetic bead dropper; 92. Trypsin dropper; 93. Suction pipe; 94. Filter screen; 10. Annular magnet. Detailed implementation manners

[0018] Next, the technical solutions in the embodiments will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments, rather than all of the embodiments. Example 1

[0019] Reference Figures 1-7 , the present invention provides a cell separation and purification device and its operation method, including an operating table 1, on which a telescopic rod 2, an ice tank 3 and an incubator 9 are installed. The ice tank 3 is used to place test tubes 4, and a robotic arm 8 is also installed on the side wall of the operating table 1; the telescopic rod 2 includes an inner tube 22 and an outer tube 21 movably sleeved outside the lower part of the inner tube 22; a crushing mechanism 5 is connected to the inner tube 22 through a connecting plate 23; a liquid storage unit for synchronously adding buffer solution is also provided in the crushing mechanism 5. The operating table 1 is also slidably installed with an annular magnet 10 and two annular grooves 7, and a timing shaking device is connected to the annular groove 7. The timing shaking device is a prior art in the art and is not shown in the figure and will not be elaborated here; specifically, a sliding groove 11 is provided at the bottom of the operating table 1, and the annular magnet 10 and the bottom of the annular groove 7 are connected with a moving plate 71. The sliding groove 11 is used to provide a sliding channel for the annular magnet 10 and the annular groove 7. A filter screen 94 is fixedly installed on the side wall of the incubator 9, and a plurality of dropper racks are slidably installed; the top of the dropper rack is connected with a flexible tube, and the flexible tube is used to transport reagents or suck liquids. In this embodiment, there are three dropper racks, located on the left side of the filter screen. The dropper racks are used to place a magnetic bead dropper 91, a trypsin dropper 92 and a liquid suction tube 93 from left to right in sequence. Among them, the dropper racks for placing the magnetic bead dropper 91 and the trypsin dropper 92 can move up and down to facilitate inserting into the test tube 4 to add corresponding reagents, and the dropper rack for placing the liquid suction tube 93 can move up and down and left and right, and can move above the filter screen 94. The robotic arm 8, the incubator 9 and its internal structures are all prior arts and will not be elaborated here.

[0020] Further, the crushing mechanism 5 includes a motor 51, a rotating shaft 52 and a cutter head 54; the motor 51 is installed on the connecting plate 23, and the rotating shaft 52 is connected to the output shaft of the motor 51; the upper and lower ends of the rotating shaft 52 are respectively provided with a first thread section 521 and a second thread section 522, and a plurality of cutter heads 54 are connected near the second thread section 522 in the middle and lower part. The motor 51 drives the rotating shaft 52 to rotate, and the cutter head 54 rotates with the rotating shaft 52, thereby cutting and crushing the tissue sample.

[0021] Furthermore, the liquid storage unit includes a limiting rod 53 and a liquid storage tube 55 mounted at the bottom of the connecting plate 23; the rotating shaft 52 and the limiting rod 53 penetrate through the liquid storage tube 55; a plurality of through holes 553 are provided on the bottom plate 552 of the liquid storage tube 55; a piston head 551 is hermetically and slidably arranged in the liquid storage tube 55, and the piston head 551 is movably sleeved on the rotating shaft 52 and the limiting rod 53; specifically, a nut structure matching the rotating shaft 52 is provided at the center of the piston head 551. When the rotating shaft 52 rotates, since the limiting rod 53 restricts the axial movement of the piston head 551, the piston head 551 can only move downward. The through holes 553 are preferably arranged to be thin and dense, which can prevent the buffer solution from leaking under its own weight in the initial state, i.e., when the piston head 551 is at the topmost position, and can also enable the buffer solution to uniformly wash the cutter head 54 when flowing downward. The motor 51 drives the rotating shaft 52 to rotate. Under the action of the limiting rod 53, the piston head 551 moves downward, pushing the buffer solution to flow downward out. The arrangement of the liquid storage tube 55 not only helps to accelerate the cutting and crushing of tissue samples, but also can keep the cells at a certain humidity, which helps to maintain cell activity.

[0022] Furthermore, the crushing mechanism 5 further includes a screening plate 56; the screening plate 56 is movably sleeved at the bottom end of the rotating shaft 52, and the limiting rod 53 penetrates through the screening plate 56; the screening plate 56 is in transmission connection with the rotating shaft 52 under the limiting action of the limiting rod 53. Specifically, a nut structure matching the second threaded section 522 is provided at the center of the screening plate 56. The screening plate 56 is preferably a screening plate in the prior art for passing cell tissues with a diameter within 1 mm. When the rotating shaft 52 rotates, since the limiting rod 53 restricts the axial movement of the screening plate 56, the screening plate 56 can only move upward or downward, which helps to accelerate the screening of cell tissues.

[0023] After the tissue sample in test tube 4 is crushed, the inner tube 22 of the telescopic rod 2 is controlled to rise, the crushing mechanism 5 is taken out, the robotic arm 8 is activated to place the test tube 4 in the ice bath 3 into the annular groove 7; the moving plate 71 is controlled to move to the right below the trypsin dropper 92, an appropriate amount of trypsin is added, the timing shaking device is activated, and it shakes once every 5 minutes. Different cell digestion times are different. After the digestion reaches the appropriate time, the timing shaking device is turned off. The moving plate 71 is controlled to move the test tube 4 below the liquid suction pipe 93. After the liquid suction pipe 93 sucks the liquid in the test tube 4, the liquid suction pipe 93 is raised, and the test tube 4 is moved to the right to be idle. Another clean test tube 4 is taken, placed in the left annular groove 7 and moved below the filter screen 94. The liquid suction pipe 93 is moved above the filter screen 94, and then the liquid in the liquid suction pipe 93 is squeezed out and filtered into the clean test tube 4 to obtain the filtrate. The filtered filtrate contains the separated single cells and larger cells, and the debris and cell clumps are filtered out. The moving plate 71 is controlled to move the test tube 4 containing the suspension below the magnetic bead dropper 91, a reagent is added, and then it is moved outside the incubator 9. The test tube 4 is placed in the annular magnet 10 by the operation of the robotic arm 8. The moving plate 71 is controlled to drive the annular magnet 10 and the test tube 4 into the incubator 9 for sorting. Example Two

[0024] On the basis of Example One, in order to avoid liquid flowing out and contaminating the operating table 1 when taking out the crushing mechanism 5, Example Two provides a preferred solution of the present invention.

[0025] Reference Figures 8-11 Referring to, the cell separation and purification device of the present invention is further provided with an anti-pollution mechanism 6. The anti-pollution mechanism 6 includes a rope 61, a spring 62 and an umbrella assembly 63; one end of the rope 61 is connected to the telescopic rod 2, and one end passes through the connecting plate 23 and the motor 51 and is then connected to the umbrella assembly 63; the spring 62 is sleeved outside the rope 61 and is located inside the rotating shaft 52, and the upper end is installed on the motor 51, and the lower end is connected to the umbrella assembly 63; the umbrella assembly 63 is sleeved inside the rotating shaft 52; the motor 51 and the rotating shaft 52 are hollow structures. The umbrella assembly 63 preferably has the structure of an umbrella in the prior art. In the initial state, the spring 62 is in a compressed state, and the umbrella assembly 63 is in a closed state. Specifically, a first limit ring 24 is provided at the bottom of the inner tube 22 of the telescopic rod 2, and a second limit ring 25 is provided at the top of the outer tube 21. The first limit ring 24 and the second limit ring 25 are matched. The second limit ring 25 is used to limit the first limit ring 24 to prevent the inner tube 22 from slipping out of the outer tube 21; an electric push rod 26 is installed at the bottom inside the outer tube 21, and a flexible limit plate 27 is provided at the top of the electric push rod 26. A third limit ring 28 for limiting the limit plate 27 is provided inside the inner tube 22; the limit plate 27 is matched with the inner tube 22 and the third limit ring 28.

[0026] When the tissue sample is crushed, the electric push rod 26 is started to extend upward, and the limit plate 27 drives the inner tube 22 and the connecting plate 23 to move upward. The crushing mechanism 5, the spring 62, and the umbrella assembly 63 move upward in the test tube 4, detach from the test tube 4, and are located directly above the test tube 4. After the first limit ring 24 contacts the second limit ring 25, the inner tube 22 no longer moves upward. When the electric push rod 26 continues to extend upward, because the limit plate 27 is flexible, the limit plate 27 continues to move upward in the inner tube 22, and the length of the rope 61 in the telescopic rod 2 is reduced. Under the elastic force of the spring 62, the length of the rope 61 in the rotating shaft 52 is lengthened, and the umbrella assembly 63 is gradually pushed out of the rotating shaft 52. When the limit plate 27 is stuck by the third limit ring 28, the umbrella assembly 63 is completely pushed out of the rotating shaft 52. Because the umbrella assembly 63 is in a closed state, it is located inside the rotating shaft 52 and does not contact the liquid surface in the test tube 4 , so the umbrella assembly 63 is in a clean state. At this time, the umbrella assembly 63 is controlled to open to prevent the tissue fluid mixture adhered to the pulverizing mechanism 5 from spilling onto the operating table 1 . Application Examples

[0027] The separation and purification of cells has many important applications in medicine, such as autologous stem cell transplantation. Stem cell technology refers to the process of isolating and purifying stem cells, culturing in vitro, directed induction, and even genetic modification, to reproduce new, normal, or even younger cells, tissues, and organs in vitro for transplantation to achieve the treatment of clinical diseases. Autologous stem cell transplantation is mainly divided into two steps: autologous stem cell collection and stem cell transplantation. The collected stem cells need to be further separated and purified to screen out specific types of stem cells for stem cell transplantation and basic research. Taking the use of tissue samples for the separation and purification of stem cells as an example, the operation method of a cell separation and purification device of the present invention is specifically described.

[0028] Step 1: Add ice cubes into the ice trough 3 to create an environment with suitable temperature, then place the test tube 4 on the ice trough 3, then place the crushing mechanism 5 into the test tube 4 to a certain depth, pour the tissue sample into the test tube 4, make the tissue sample stay on the screening plate 56, and then completely place the part below the liquid storage tube 55 of the crushing mechanism 5 into the test tube 4.

[0029] Step 2: Start the motor 51 to drive the shaft 52 to rotate, and the cutter head 54 rotates around the shaft 52 to cut and crush the tissue sample; at the same time, the piston head 551 moves downward under the driving action of the shaft 52 to squeeze out the buffer in the liquid storage tube 55, and the buffer washes the tissue on the cutter head 54 and flows into the test tube 4 through the screening plate 56; at the same time, the screening plate 56 moves upward under the driving action of the shaft 52 to further speed up the screening. After the tissue sample is crushed, turn off the motor 51.

[0030] Step 3: Start the electric push rod 26, and the limiting plate 27 pushes the inner tube 22 to move upward, driving the connecting plate 23 and the crushing mechanism 5 to move upward; when the first limiting ring 24 contacts the second limiting ring 25, the inner tube 22 stops moving upward, and the limiting plate 27 enters the inner tube 22 and continues to move upward. At this time, the connecting plate 23 and the crushing mechanism 5 also stop moving, the length of the rope 61 located inside the telescopic rod 2 gradually decreases, and the length of the rope 61 located inside the rotating shaft 52 gradually increases. Under the elastic force of the spring 62, the umbrella assembly 63 is gradually pushed out of the rotating shaft 52; when the limiting plate 27 contacts the third limiting ring 28, the umbrella assembly 63 is completely pushed out of the rotating shaft 52. At this time, control the umbrella assembly 63 to open to prevent the tissue fluid mixture adhering to the crushing mechanism 5 from spilling onto the operating table 1.

[0031] Step 4: Rotate the inner tube 22 to rotate the crushing mechanism 5 above the test tube 4. Operate the robotic arm 8 to move the test tube 4 into the right annular groove 7. Move the moving plate 71 to move the test tube 4 under the pancreatic enzyme dropper 92, add an appropriate amount of pancreatic enzyme reagent to the test tube 4, start the timing shaking device, and shake it once every 5 minutes. Different cell digestion times are different. After waiting for the appropriate digestion time, turn off the timing shaking device.

[0032] Step 5: Control the moving plate 71 to move the test tube 4 under the liquid suction tube 93. After the liquid suction tube 93 sucks the liquid in the test tube 4, raise the liquid suction tube 93, and move the test tube 4 to the right for idling. Take another clean test tube 4, place it in the left annular groove 7 and move it under the filter screen 94. Move the liquid suction tube 93 above the filter screen 94, and then squeeze the liquid in the liquid suction tube 93 to filter and enter the clean test tube 4 to obtain the filtrate.

[0033] Step 6: Control the moving plate 71 to move the test tube 4 containing the suspension under the magnetic bead dropper 91, add the reagent, and then move it outside the incubator 9. Operate the robotic arm 8 to place the test tube 4 in the annular magnet 10, and control the moving plate 71 to drive the annular magnet 10 and the test tube 4 into the incubator 9 for sorting and collection.

[0034] Step 7: Perform quality inspection on the collected cells to ensure that they meet the experimental requirements.

[0035] It should be clear that the above detailed description of the embodiments of the present invention provided in the drawings is not intended to limit the scope of the claimed invention, but merely represents selected embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the scope of protection of the present invention.

Claims

1. A cell separation and purification device, comprising an operation table (1) and an incubator (9) installed on the operation table (1); It is characterized in that: The operation table (1) is further installed with a telescopic rod (2), an ice tank (3), a robotic arm (8), as well as a slidable annular groove (7) and an annular magnet (10); The ice tank (3), the annular groove (7) and the annular magnet (10) are used for placing test tubes (4); A crushing mechanism (5) is connected to the telescopic rod (2), and a liquid storage unit for synchronously adding a buffer solution is further arranged in the crushing mechanism (5).

2. The cell separation and purification device according to claim 1, wherein: The crushing mechanism (5) includes a motor (51), a rotating shaft (52) and a cutter head (54); The motor (51) is connected to the telescopic rod (2) through a connecting plate (23); The rotating shaft (52) is connected to the output shaft of the motor (51); The cutter head (54) is arranged on the rotating shaft (52).

3. The cell separation and purification device according to claim 2, wherein: The liquid storage unit includes a limiting rod (53) and a liquid storage pipe (55) installed on the connecting plate (23); The rotating shaft (52) penetrates through the liquid storage pipe (55); A plurality of through holes (553) are arranged on the bottom plate (552) of the liquid storage pipe (55); A piston head (551) is hermetically and slidably arranged in the liquid storage pipe (55), and the piston head (551) is sleeved on the rotating shaft (52) and the limiting rod (53).

4. The cell separation and purification device according to claim 3, characterized in that: The crushing mechanism (5) further includes a screening plate (56); The screening plate (56) is sleeved on the bottom end of the rotating shaft (52), and the limiting rod (53) penetrates through the screening plate (56).

5. The cell separation and purification device according to claim 2, characterized in that: An anti-pollution mechanism (6) is further arranged between the telescopic rod (2) and the crushing mechanism (5), and the anti-pollution mechanism (6) is used for preventing liquid from flowing out and polluting the operation table (1) when the crushing mechanism (5) is taken out.

6. The cell separation and purification device according to claim 5, wherein: The telescopic rod (2) includes an inner tube (22) and an outer tube (21) movably sleeved outside the lower part of the inner tube (22); The inner tube (22) is connected to the connecting plate (23); The outer tube (21) is installed on the operation table (1).

7. The cell separation and purification device according to claim 6, wherein: An electric push rod (26) is installed in the outer tube (21); A first limiting ring (24) is arranged at the bottom of the inner tube (22); A flexible limiting plate (27) is arranged at the top of the electric push rod (26); The limiting plate (27) is matched with the first limiting ring (24); A third limiting ring (28) matched with the limiting plate (27) is arranged in the inner tube (22); The limiting plate (27) is connected to the anti-pollution mechanism (6).

8. A cell separation and purification device according to claim 7, characterized in that: The anti-pollution mechanism (6) includes a rope (61), a spring (62) and an umbrella assembly (63); One end of the rope (61) is connected to the limiting plate (27), and the other end passes through the connecting plate (23) and the motor (51) and is connected to the umbrella assembly (63); The spring (62) is sleeved outside the rope (61), and the upper end is installed on the motor (51), and the lower end is connected to the umbrella assembly (63); The spring (62) and the umbrella assembly (63) are sleeved in the rotating shaft (52).

9. The operating method of the cell separation and purification device according to any one of claims 1-8, characterized in that: Including the following steps, S1: Place the test tube (4) on the ice bath (3), start the crushing mechanism (5) to crush the tissue sample, and add buffer solution to the test tube (4) while crushing; S2: Start the telescopic rod and move the crushing mechanism (5) out of the test tube (4); S3: Operate the robotic arm (8) to place the test tube (4) into the annular groove (7), move the annular groove (7) into the incubator (9), complete the digestion and filtration of the cells, and then move the test tube (4) out of the incubator (9); S4: Operate the robotic arm (8), place the test tube (4) into the annular magnet (10), move the annular magnet (10) into the incubator (9), and complete the purification.

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