Improved immunohistochemical double staining equipment
Through the layered introduction of the design of the mechanism and humidification mechanism, the samples are confused and humidity instability are solved, and the experimental efficiency and accuracy of the immunohistochemistry dual staining equipment are improved.
Patent Information
- Application Number
- CN202510505936.8
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-04-22
- Publication Date
- 2025-07-11
- Estimated Expiration
- Not applicable · inactive patent
AI Technical Summary
In the prior art, when multiple sample experimental wet boxes are placed on the same horizontal plane, samples are easily confused, affecting the accuracy of experimental results and data integrity.
The layered roll-out mechanism and humidification mechanism are used to store the experimental wet box in layers, and the layered movement of the wet box is achieved through the motor-driven threaded rod and rack structure. Combined with the humidifier, the constant humidity environment is maintained to avoid the risk of sample confusion and drying.
The wet experimental box is stored in layers and conveniently removed, which improves the experimental efficiency and accuracy, reduces the risk of sample confusion, and ensures the reliability and quality of experimental results.
Smart Images
Figure CN120293637A_ABST
Abstract
Description
Technical Field
[0001] The present invention relates to the technical field of equipment for immunohistological experiments, and particularly to an improved immunohistochemical double staining device. Background Art
[0002] Immunohistochemical double staining experiment is a commonly used technique in biomedical and clinical research practice, aiming to detect whether two different substances exist in the same sample or the same kind of cells. Immunohistochemical double staining experiment is a complex experimental technique, which needs to be carried out under specific humidity and temperature conditions to ensure the accuracy and reliability of experimental results. Breast cancer is one of the major malignant tumors threatening women's health
[0003] [1], ranking first among female malignant tumors [2]. It is the most common malignant tumor among women in the world, with an incidence rate accounting for 23% of malignant tumors and a mortality rate accounting for 14% of malignant tumors [3]. Early detection plays an important role in improving the survival rate of breast cancer. In recent years, the number of breast cancer patients in China has been on the rise, and there is also a trend of getting younger. The 5-year survival rate of breast cancer patients is 50% - 60%, and most patients have characteristics such as recurrence and metastasis. The average survival time of advanced breast cancer patients is only 18 - 30 months [4 - 5]. Therefore, how to accurately diagnose the nature and progression of breast tumors by applying more sensitive and specific tumor markers is an important topic related to the health of Chinese women.
[0004] In the prior art, when staff need to conduct multiple experiments, multiple sample experiment wet boxes are usually placed on the same horizontal plane. When it is necessary to process multiple samples simultaneously and the staff is relatively busy, if the labels are not clear or the management is poor, it is very easy to have the situation of sample confusion. This kind of confusion may lead to misinterpretation of experimental results or data loss. Therefore, an improved immunohistochemical double staining device is proposed. Summary of the Invention
[0005] The purpose of the present invention is to solve the disadvantages existing in the prior art that when staff need to conduct multiple experiments, multiple sample experiment wet boxes are usually placed on the same horizontal plane. When it is necessary to process multiple samples simultaneously and the staff is relatively busy, if the labels are not clear or the management is poor, it is very easy to have the situation of sample confusion. This kind of confusion may lead to misinterpretation of experimental results or data loss, and to propose an improved immunohistochemical double staining device.
[0006] In order to achieve the above purpose, the present invention adopts the following technical scheme:
[0007] An improved immunohistochemical double staining device, comprising an experimental box. A layered pushing mechanism is arranged inside the experimental box. The layered pushing mechanism includes a first motor arranged inside the experimental box. A threaded rod is arranged at the output end of the first motor. A first experimental plate is threadedly connected above the threaded rod. A first gear is drivingly connected inside one side of the first experimental plate close to the experimental box. A first rack plate is meshingly connected to one side of the first gear close to the experimental box. A third rack plate is meshingly connected to one side of the first gear away from the first rack plate. A second rack plate is fixedly connected to one side of the first experimental plate close to the third rack plate. A second experimental plate is fixedly connected to the side of the third rack plate. A second gear is meshingly connected to one side of the second rack plate close to the second experimental plate. A fourth rack plate is meshingly connected to one side of the second gear away from the second rack plate. A third experimental plate is fixedly connected to the side of the fourth rack plate. After the first motor is started, the first experimental plate is driven to move by the threaded rod. The first experimental plate drives the second experimental plate to move through the meshing connection relationship among the first gear, the first rack plate and the third rack plate. The second experimental plate drives the third experimental plate to move through the meshing connection relationship among the second rack plate, the second gear and the fourth rack plate. The first experimental plate, the second experimental plate and the third experimental plate move out of the experimental box simultaneously.
[0008] The above technical solution further includes:
[0009] The experimental box is fixedly connected to the first motor. The first rack plate is fixedly connected to the experimental box. A square groove is formed inside the first experimental plate. The square groove is rotatably connected to the first gear. The first experimental plate is slidably connected to the experimental box. The first experimental plate is slidably connected to the second experimental plate. The second experimental plate is slidably connected to the third experimental plate.
[0010] An experimental wet box is arranged above the third experimental plate. Experimental wet boxes of different specifications are arranged above the first experimental plate and the second experimental plate.
[0011] Among them, the experimental wet box uses a cocktail combination of primary antibodies, which can simultaneously detect the expression of three antigens, CK7, P63, and Calponin, on the same tissue section, reducing the sample usage, avoiding the differences caused by different sections, and being able to visually and clearly distinguish normal tissues and cancer tissues, providing more comprehensive and reliable diagnostic basis for pathologists, and improving the detection rate of early invasive breast cancer and micro-invasive breast cancer. It includes a peroxidase blocker, a combination of primary antibodies, a combination of secondary antibodies, a DAB chromogenic substrate, a DAB chromogenic buffer, a fast red chromogenic agent, a fast red chromogenic activator, a fast red buffer, and a hematoxylin counterstain solution. Among them, the combination of primary antibodies includes a rabbit anti-human cytokeratin 7 monoclonal antibody, a mouse anti-human P63 monoclonal antibody, and a mouse anti-human calponin monoclonal antibody, and the combination of secondary antibodies includes an HRP-labeled anti-mouse secondary antibody polymer and an AP-labeled anti-rabbit secondary antibody polymer.
[0012] A humidifying cylinder is fixedly connected to the side of the experimental box.
[0013] A humidifying mechanism is arranged inside the humidifying cylinder, and the humidifying mechanism includes a second motor arranged inside the humidifying cylinder.
[0014] A driving rod is arranged at the output end of the second motor. A ventilation cylinder is fixedly connected to the side of the driving rod away from the second motor. A power fan is fixedly connected to the side of the ventilation cylinder. A humidifier is arranged on the side of the humidifying cylinder away from the second motor.
[0015] The bottom of the second motor is fixedly connected to a machine box. The machine box is fixedly connected to the humidifying cylinder. The driving rod is rotatably connected to the machine box. The power fan is rotatably connected to the humidifying cylinder.
[0016] An inlet cylinder is fixedly connected to the side of the humidifying cylinder away from the experimental box. A telescopic rod is arranged on the side of the inlet cylinder away from the humidifying cylinder. A baffle is fixedly connected to the side of the telescopic rod away from the inlet cylinder.
[0017] A humidity monitor is fixedly installed on the inner wall of the experimental box.
[0018] A door panel is rotatably connected to the side of the experimental box. An observation glass plate is embedded in the door panel. A handle is fixedly connected to the side of the door panel close to the observation glass plate.
[0019] The present invention has the following beneficial effects:
[0020] 1. In the present invention, through the arranged layered pushing-out mechanism, the layered storage and convenient taking-out of the experimental wet box are realized. The first motor drives the threaded rod to rotate, thereby driving the first experimental plate, the second experimental plate, and the third experimental plate to move to different positions respectively, enabling the staff to easily take the required experimental wet box at different positions and heights according to the sample type or experimental requirements, effectively avoiding sample confusion, and improving the experimental efficiency and accuracy.
[0021] 2. In the present invention, a humidifying mechanism is provided. The power fan is driven by the second motor to rotate and generate suction, quickly transferring the moisture generated by the humidifier to the inside of the experimental chamber, thereby maintaining a constant humidity environment required for the experiment. This helps to reduce the drying risk of the sample during the staining process and improve the quality and effect of staining. BRIEF DESCRIPTION OF THE DRAWINGS
[0022] Figure 1 It is a schematic structural diagram of an improved immunohistochemical double staining device proposed by the present invention;
[0023] Figure 2 It is an external structural diagram of the present invention;
[0024] Figure 3 It is a front view schematic diagram of the internal three-dimensional structure of the present invention;
[0025] Figure 4 It is a top view schematic diagram of the internal three-dimensional mechanism of the present invention;
[0026] Figure 5 It is a schematic diagram of the internal structure of the humidifying cylinder of the present invention;
[0027] Figure 6 It is Figure 1 a schematic enlarged view of the structure at B in
[0028] Figure 7 It is Figure 5 a schematic enlarged view of the structure at C in
[0029] In the figure: 1. Experimental chamber; 2. First motor; 3. Threaded rod; 4. First experimental plate; 5. First gear; 6. First rack plate; 7. Square groove; 8. Second rack plate; 9. Third rack plate; 10. Second experimental plate; 11. Second gear; 12. Fourth rack plate; 13. Third experimental plate; 14. Experimental wet box; 15. Humidifying cylinder; 16. Chassis; 17. Second motor; 18. Driving rod; 19. Air duct; 20. Power fan; 21. Inlet tube; 22. Telescopic rod; 23. Baffle; 24. Humidifier; 25. Humidity monitor; 26. Door panel; 27. Observation glass plate; 28. Handle. DETAILED DESCRIPTION OF THE EMBODIMENTS
[0030] Next, the technical solutions in the embodiments of the present invention will be clearly and completely described in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.
[0031] Embodiment 1
[0032] As Figures 1 - 7 shown, an improved immunohistochemical double staining device proposed by the present invention includes an experimental box 1. A hierarchical pushing mechanism is arranged inside the experimental box 1. The hierarchical pushing mechanism includes a first motor 2 arranged inside the experimental box 1. A threaded rod 3 is arranged at the output end of the first motor 2. A first experimental plate 4 is threadedly connected above the threaded rod 3. A first gear 5 is drivingly connected inside one side of the first experimental plate 4 close to the experimental box 1. A first rack plate 6 is meshingly connected to one side of the first gear 5 close to the experimental box 1. A third rack plate 9 is meshingly connected to one side of the first gear 5 away from the first rack plate 6. A second rack plate 8 is fixedly connected to one side of the first experimental plate 4 close to the third rack plate 9. A second experimental plate 10 is fixedly connected to the side of the third rack plate 9. A second gear 11 is meshingly connected to one side of the second rack plate 8 close to the second experimental plate 10. A fourth rack plate 12 is meshingly connected to one side of the second gear 11 away from the second rack plate 8. A third experimental plate 13 is fixedly connected to the side of the fourth rack plate 12. After the first motor 2 is started, the first experimental plate 4 is driven to move by the threaded rod 3. The first experimental plate 4 drives the second experimental plate 10 to move through the meshing connection relationship among the first gear 5, the first rack plate 6 and the third rack plate 9. The second experimental plate 10 drives the third experimental plate 13 to move through the meshing connection relationship among the second rack plate 8, the second gear 11 and the fourth rack plate 12. The first experimental plate 4, the second experimental plate 10 and the third experimental plate 13 move out of the experimental box 1 simultaneously.
[0033] The experimental box 1 is fixedly connected to the first motor 2. The first rack plate 6 is fixedly connected to the experimental box 1. A square groove 7 is opened inside the first experimental plate 4. The square groove 7 is rotatably connected to the first gear 5. The first experimental plate 4 is slidably connected to the experimental box 1. The first experimental plate 4 is slidably connected to the second experimental plate 10. The second experimental plate 10 is slidably connected to the third experimental plate 13.
[0034] An experimental wet box 14 is arranged above the third experimental plate 13. Experimental wet boxes 14 with different specifications are arranged above the first experimental plate 4 and the second experimental plate 10.
[0035] A humidifying cylinder 15 is fixedly connected to the side of the experimental box 1.
[0036] A humidifying mechanism is arranged inside the humidifying cylinder 15. The humidifying mechanism includes a second motor 17 arranged inside the humidifying cylinder 15.
[0037] A driving rod 18 is arranged at the output end of the second motor 17. An air passing cylinder 19 is fixedly connected to the side of the driving rod 18 away from the second motor 17. A power fan 20 is fixedly connected to the side of the air passing cylinder 19. A humidifier 24 is arranged on the side of the humidifying cylinder 15 away from the second motor 17.
[0038] The bottom of the second motor 17 is fixedly connected to the chassis 16. The chassis 16 is fixedly connected to the humidifying cylinder 15. The driving rod 18 is rotatably connected to the chassis 16. The power fan 20 is rotatably connected to the humidifying cylinder 15.
[0039] In this embodiment, the hierarchical protection mechanism provided inside the experimental box 1 is started. The first motor 2 fixedly installed on the inner wall of the bottom of the experimental box 1 starts to operate. When the first motor 2 operates, the threaded rod 3 provided at its output end is controlled to rotate. The threaded rod 3 is threadedly connected to the first experimental plate 4. Restricted by the sliding connection between the first experimental plate 4 and the experimental box 1, the first experimental plate 4 moves under the influence of the threaded power. When the first experimental plate 4 moves, it drives the first gear 5 rotatably connected to its interior to rotate. The first gear 5 rotates inside the square groove 7 opened inside the first experimental plate 4. Since the first gear 5 is meshed with the first rack plate 6 and the third rack plate 9, the first gear 5 rotates during the movement, thereby driving the third rack plate 9 to move through the meshing connection relationship. When the third rack plate 9 moves, it drives the second experimental plate 10 fixedly connected to its side to move. At the same time as the first experimental plate 4 moves, it drives the second rack plate 8 to move. When the second rack plate 8 moves, it drives the second gear 11 meshed with its side to rotate, causing the second gear 11 to make the fourth rack plate 12 move under the influence of the meshing connection relationship with the fourth rack plate 12. When the fourth rack plate 12 moves, it drives the third experimental plate 13 to move, so that the first experimental plate 4, the second experimental plate 10, and the third experimental plate 13 move to different positions respectively. The third experimental plate 13 drives the experimental wet box 14 provided above it to start moving. The second experimental plate 10 and the third experimental plate 13 drive other specifications of experimental wet boxes 14 to move. Since the first experimental plate 4, the second experimental plate 10, and the third experimental plate 13 are set at different heights, the hierarchical storage function is realized, enabling the staff to pick up the experimental wet boxes 14 at different positions and heights according to the type, preventing specimen confusion and improving the picking efficiency.
[0040] When the humidity inside the experimental chamber 1 is insufficient, the humidifying mechanism provided inside the humidifying cylinder 15 can be activated. The second motor 17 in the humidifying mechanism starts to operate. The second motor 17 is fixedly installed on the inner wall of the chassis 16. The chassis 16 is fixedly connected to the humidifying cylinder 15. When the second motor 17 operates, it starts to control the driving rod 18 provided at its output end to rotate. The other end of the driving rod 18 is fixedly connected to the air passing cylinder 19. When the driving rod 18 rotates, it drives the air passing cylinder 19 to start rotating. When the air passing cylinder 19 rotates, it drives the power fan 20 fixedly connected to its side to rotate. The power fan 20 starts to rotate on the inner wall of the humidifying cylinder 15 and generates suction. Moisture is generated by the humidifier 24 fixedly installed at the other end of the humidifying cylinder 15. The suction generated by the rotation of the power fan 20 quickly transfers the moisture generated by the humidifier 24 into the experimental chamber 1 for humidity adjustment.
[0041] Embodiment 2
[0042] As Figures 1 - 7 shown, on the basis of Embodiment 1, an inlet cylinder 21 is fixedly connected to the side of the humidifying cylinder 15 away from the experimental chamber 1. An expansion rod 22 is provided on the side of the inlet cylinder 21 away from the humidifying cylinder 15. A baffle 23 is fixedly connected to the side of the expansion rod 22 away from the inlet cylinder 21.
[0043] A humidity monitor 25 is fixedly installed on the inner wall of the experimental chamber 1.
[0044] A door panel 26 is rotatably connected to the side of the experimental chamber 1. An observation glass plate 27 is embedded in the door panel 26. A handle 28 is fixedly connected to the side of the door panel 26 close to the observation glass plate 27.
[0045] In this embodiment, when the adjustment mechanism is not in use, an inlet cylinder 21 is fixedly connected to the end of the humidifying cylinder 15 away from the humidifier 24. By controlling the contraction of the expansion rods 22 evenly distributed inside the inlet cylinder 21, multiple groups of expansion rods 22 drive the baffle 23 to move until it completely fits with the inlet cylinder 21, so that the space between the experimental chamber 1 and the humidifying cylinder 15 is sealed, preventing dry air from entering the experimental chamber 1 and affecting the immunohistochemical double experiment process. The humidity monitor 25 provided inside the experimental chamber 1 is used to monitor the humidity change inside the experimental chamber 1, so that the staff can adjust it in time. A door panel 26 is rotatably connected to the side of the experimental chamber 1. The door panel 26 is opened and closed by pulling the handle 28. The observation glass plate 27 embedded in the door panel 26 is used for the staff to conduct real-time experiment observations.
[0046] Although the embodiments of the present invention have been shown and described, for those of ordinary skill in the art, it can be understood that various changes, modifications, substitutions, and variations can be made to these embodiments without departing from the principles and spirit of the present invention. The scope of the present invention is defined by the appended claims and their equivalents.
Claims
1. An improved immunohistochemical double staining device, including an experimental box (1), characterized in that, A layered pushing mechanism is arranged inside the experimental box (1). The layered pushing mechanism includes a first motor (2) arranged inside the experimental box (1). A threaded rod (3) is arranged at the output end of the first motor (2). A first experimental plate (4) is threadedly connected above the threaded rod (3). A first gear (5) is drivingly connected inside one side of the first experimental plate (4) close to the experimental box (1). A first rack plate (6) is meshingly connected to one side of the first gear (5) close to the experimental box (1). A third rack plate (9) is meshingly connected to one side of the first gear (5) away from the first rack plate (6). A second rack plate (8) is fixedly connected to one side of the first experimental plate (4) close to the third rack plate (9). A second experimental plate (10) is fixedly connected to the side of the third rack plate (9). A second gear (11) is meshingly connected to one side of the second rack plate (8) close to the second experimental plate (10). A fourth rack plate (12) is meshingly connected to one side of the second gear (11) away from the second rack plate (8). A third experimental plate (13) is fixedly connected to the side of the fourth rack plate (12). After the first motor (2) is started, the first experimental plate (4) is driven to move by the threaded rod (3). The first experimental plate (4) drives the second experimental plate (10) to move through the meshing connection relationship among the first gear (5), the first rack plate (6) and the third rack plate (9). The second experimental plate (10) drives the third experimental plate (13) to move through the meshing connection relationship among the second rack plate (8), the second gear (11) and the fourth rack plate (12). The first experimental plate (4), the second experimental plate (10) and the third experimental plate (13) simultaneously move outside the experimental box (1).
2. The improved immunohistochemical double staining device according to claim 1, wherein, The experimental box (1) is fixedly connected to the first motor (2). The first rack plate (6) is fixedly connected to the experimental box (1). A square groove (7) is formed inside the first experimental plate (4). The square groove (7) is rotatably connected to the first gear (5). The first experimental plate (4) is slidably connected to the experimental box (1). The first experimental plate (4) is slidably connected to the second experimental plate (10). The second experimental plate (10) is slidably connected to the third experimental plate (13).
3. An improved immunohistochemical double staining device according to claim 1, characterized in that, An experimental wet box (14) is arranged above the third experimental plate (13). Experimental wet boxes (14) with different specifications are arranged above the first experimental plate (4) and the second experimental plate 1(0).
4. An improved immunohistochemical double staining device according to claim 1, characterized in that, A humidifying cylinder (15) is fixedly connected to the side of the experimental box (1).
5. An improved immunohistochemical double staining device according to claim 4, characterized in that, A humidifying mechanism is arranged inside the humidifying cylinder (15). The humidifying mechanism includes a second motor (17) arranged inside the humidifying cylinder (15).
6. An improved immunohistochemical double staining device according to claim 5, characterized in that, The output end of the second motor (17) is provided with a driving rod (18). One side of the driving rod (18) far from the second motor (17) is fixedly connected with an air passing cylinder (19). One side of the air passing cylinder (19) is fixedly connected with a power fan (20). One side of the humidifying cylinder (15) far from the second motor (17) is provided with a humidifier (24).
7. An improved immunohistochemical double staining device according to claim 6, characterized in that, The bottom of the second motor (17) is fixedly connected with a machine box (16). The machine box (16) is fixedly connected with the humidifying cylinder (15). The driving rod (18) is rotatably connected with the machine box (16). The power fan (20) is rotatably connected with the humidifying cylinder (15).
8. An improved immunohistochemical double staining device according to claim 4, characterized in that, One side of the humidifying cylinder (15) far from the experimental box (1) is fixedly connected with an inlet cylinder (21). One side of the inlet cylinder (21) far from the humidifying cylinder (15) is provided with a telescopic rod (22). One side of the telescopic rod (22) far from the inlet cylinder (21) is fixedly connected with a baffle (23).
9. An improved immunohistochemical double staining device according to claim 1, characterized in that, A humidity monitor (25) is fixedly installed on the inner wall of the experimental box (1).
10. An improved immunohistochemical double staining device according to claim 1, characterized in that, One side of the experimental box (1) is rotatably connected with a door panel (26). An observation glass plate (27) is embedded in the door panel (26). One side of the door panel (26) close to the observation glass plate (27) is fixedly connected with a handle (28).