Method for preparing amorphous photonic crystal microspheres by using super-amphiphobic surface

Abstract

The invention belongs to the technical field of material synthesis, and particularly discloses a method for preparing amorphous photonic crystal microspheres by using a super-amphiphobic surface, which comprises the following steps: pre-forming a poly (styrene-methyl methacrylate-acrylic acid) nano-microsphere emulsion by using centrifugal operation, pre-crystallizing, and carrying out evaporation induced self-assembly on the super-amphiphobic surface to obtain the amorphous photonic crystal microspheres. According to the preparation method, the amorphous photonic crystal microspheres which are in a short-range ordered and long-range disordered amorphous state, are highly spheroidal and are uniform can be rapidly formed, and the amorphous photonic crystal microspheres which are uniform in structure, stable in performance and simple in component can be prepared in a large scale by adopting the preparation method. Therefore, the used materials are simple, the cost is low, the preparation process is simple and rapid, the operability is high, and the preparation method is easy to implement and easy to industrialize and use on a large scale.

Description

Technical Field

[0001] The present invention relates to the technical field of material synthesis, and in particular to a method for preparing amorphous photonic crystal microspheres by using a superhydrophobic and superoleophobic surface. Background Art

[0002] Due to the periodic micro-nano structure, photonic crystals have an important influence on the propagation behavior of photons, resulting in the unique phenomenon of structural color. The structural color of traditional photonic crystals shows obvious angular dependence, and the color changes with the angle of incident light. Amorphous photonic crystals have a short-range disordered and long-range ordered micro-nano structure, making their structural color properties independent of angle and having broad prospects in applications such as biological detection and structural color pigments. Existing technologies often use methods such as spraying, chemical etching, and self-assembly to prepare amorphous photonic crystal microspheres, thin films, and pigment powders. The superhydrophobic and superoleophobic surface has a large contact angle and a low rolling angle, which can make the droplets on the surface form highly spherical shapes and prevent the droplets from being pinned by the solid-liquid-gas triple-phase line during the evaporation and drying process of droplet shrinkage. Therefore, it is widely used in the preparation of photonic crystal microspheres by evaporation-induced self-assembly technology.

[0003] For example, a method for synthesizing microspheres on a superhydrophobic and superoleophobic surface disclosed in Chinese Patent Publication No. CN108355588A includes the following steps: a. providing a substrate with at least one surface being a superhydrophobic and superoleophobic surface; b. providing a droplet cured on the superhydrophobic and superoleophobic surface of the substrate; c. curing the droplet on the superhydrophobic and superoleophobic surface of the substrate to form microspheres. The method of the present invention is novel, simple, and effective, without the use of emulsifiers, without complex equipment, and can flexibly adapt to the synthesis of different types of microspheres.

[0004] For example, a method for preparing polymer microspheres on a superhydrophobic and superoleophobic surface disclosed in Chinese Patent Publication No. CN111892722A includes the following steps: fabricating a superhydrophobic and superoleophobic surface on a substrate; preparing a polymer spinning solution with a polymer material, and spinning a polymer fiber array on the superhydrophobic and superoleophobic surface by microfluidic spinning; using laser thermal cutting to cut the polymer fiber array to obtain several segments of polymer short fibers; heating the substrate with the attached polymer short fibers until the polymer short fibers melt to form a spherical structure, and then cooling to obtain the product. This method can effectively solve the problems that the existing methods have expensive preparation equipment and the structural morphology of the prepared polymer microspheres is difficult to meet the requirements, and can flexibly control the size of the target polymer microspheres.

[0005] A method for preparing amorphous photonic crystal microspheres with controllable morphology and angle-independent color, as disclosed in Chinese Patent Publication No. CN105177714A, mixes Fe3O4 nanoparticles, carbon black nanoparticles, graphene nanoparticles or a mixture of the three with an emulsion containing monodisperse colloidal microspheres, and sprays, drops or inkjet prints the mixture onto a superhydrophobic substrate to form emulsion drops with a diameter of 11 μm to 1.6 mm. After drying, spherical or concave spherical angle-independent color photonic crystal microspheres are obtained; alternatively, Fe3O4 nanoparticles or carbon black nanoparticles or graphene nanoparticles or a mixture of the three, ethanol or a surfactant are mixed with an emulsion containing monodisperse colloidal microspheres, and then sprayed, dropped or inkjet printed onto a superhydrophobic substrate to form emulsion drops with a diameter of 11 μm to 1.6 mm. After drying, ellipsoidal angle-independent color photonic crystal microspheres are obtained, and the aspect ratio of the microspheres is controllable. It has the characteristics of non-fading and environmental protection, and has broad application prospects in the fields of pigments, displays, sensors, etc.

[0006] However, the microspheres prepared by the above-mentioned super-biphobic surface are mostly used for the synthesis of polymer microspheres or the curing of other polymers, and less attention is paid to the preparation of amorphous photonic crystals, which are special photonic crystals with angle-independent structural color properties. Moreover, in the existing research technologies for angle-independent color photonic crystal microspheres, the components are relatively complex and the raw material costs required are high. The above problems have limited the application of amorphous photonic crystal microspheres in biological detection and structural color pigments to a certain extent. Summary of the Invention

[0007] In order to solve the above technical problems, the present invention provides a method for preparing amorphous photonic crystal microspheres using a super-biphobic surface, and the prepared microspheres are uniform and have angle-independent structural color properties.

[0008] To achieve the above object, the present invention is implemented according to the following technical scheme:

[0009] The first technical solution proposed by the present invention is a method for preparing amorphous photonic crystal microspheres using a super-biphobic surface, including the following steps:

[0010] S1. Mix a poly(styrene-methyl methacrylate-acrylic acid) nanosphere emulsion with a volume ratio of 1:4 and deionized water, centrifuge to remove the supernatant, and ultrasonically oscillate the lower emulsion to be uniform to obtain an emulsion with structural color.

[0011] S2. Under the conditions of a temperature of 21°C and a humidity of 65%, drop the emulsion with structural color onto an aluminum sheet with a transparent super-biphobic coating, and let it stand to allow the emulsion with structural color to self-assemble to form amorphous photonic crystal microspheres.

[0012] Furthermore, the preparation process of the poly(styrene-methyl methacrylate-acrylic acid) nanosphere emulsion is as follows:

[0013] 1) First, perform vacuum distillation of styrene to remove the polymerization inhibitor: add styrene and a 5% sodium hydroxide solution by mass into a volumetric flask, wash it upside down twice until it is colorless, let it stand for stratification, separate and remove the upper liquid with a separatory funnel, then wash it with ultrapure water until it is neutral, add 0.5% cuprous chloride during distillation, first heat it in a water bath at 60°C to remove the front distillate component, then heat it to 68°C to 75°C to collect the main distillate, and obtain styrene with the polymerization inhibitor removed, cool it at room temperature, and store it in a refrigerator for later use;

[0014] 2) Synthesis reaction: 2g of 1g / L emulsifier sodium dodecylbenzene sulfonate solution, 0.5g of ammonium bicarbonate solution and the remaining ultrapure water (80mL) were placed in a three-necked flask, heated in a water bath at 60°C and stirred thoroughly with a magnetic stirring paddle at a stirring speed of 350rpm; then 19g of redistilled styrene, 1g of methyl methacrylate and 1g of acrylic acid were added, the stirring speed was maintained at 350rpm and the temperature was raised to 60°C and maintained for 1h, and then the temperature was raised to 70°C and maintained for 30min; 12mL of initiator ammonium persulfate solution was slowly added at a rate of 3s / drop, and the temperature was stirred at a constant temperature for 1.5h; after the temperature was raised to 80°C, 4mL of APS solution was slowly added at the same rate, and the temperature was stirred for 2h; finally, 4mL of APS solution was slowly added again, and the reaction was continued for 2h; after the reaction was completed, the suspension was taken out, cooled and bottled to obtain a monodisperse poly(styrene-methyl methacrylate-acrylic acid) nanosphere suspension.

[0015] Furthermore, in step S1, the centrifugal speed is 8000 rpm and the centrifugal time is 60 min.

[0016] Furthermore, in step S2, the preparation process of the transparent super-amphiphobic coating is as follows:

[0017] 1) Preparation of nanosphere mixed silica sol: Anhydrous ethanol and a monodisperse polystyrene nanosphere emulsion with a diameter of 50 nm and a mass concentration of 5% were mixed in a mass ratio of 5:1 to prepare liquid A; tetraethyl silicate TEOS, 0.1 mol / L hydrochloric acid and anhydrous ethanol were mixed in a mass ratio of 1:1:1.5 to prepare liquid B; 6 g of liquid A and 0.2 g of liquid B were oscillated and mixed, and stirred for 40 minutes using a magnetic stirrer, and then the partially hydrolyzed prepolymer solution was ultrasonically dispersed for 15 minutes to prepare a nanosphere mixed silica sol;

[0018] 2) Pretreatment of aluminum sheet: Clean the aluminum sheet, then soak it in anhydrous ethanol solution and ultrasonicate for 30 min, then put the aluminum sheet into deionized water and ultrasonicate for 10 min. After taking it out, dry it with nitrogen or compressed air. The treated aluminum sheet is soaked in 1 mol / L hydrochloric acid for 1 min, taken out and washed clean with ultrapure water, and then dried again with nitrogen or compressed air. After that, it is treated by plasma for 5 min, taken out and stored away from dust for standby;

[0019] 3) Spraying: Place the pretreated aluminum sheet on a hot plate at 85 °C and heat for 5 min or until the surface temperature is stable. Then, under the conditions of setting the pressure of the spray gun at 0.40 MPa and the distance from the aluminum sheet surface at 15 cm, spray the prepared nano-sphere mixed silica sol on the aluminum sheet surface to cure into a film;

[0020] 4) Calcination: Transfer the aluminum sheet with a cured film on the surface to a muffle furnace, heat it at a heating rate of 2 °C / min to 500 °C and hold for 2 h, and then naturally cool to obtain an aluminum sheet with a transparent coating;

[0021] 5) Low surface energy modification: After the aluminum sheet with a transparent coating prepared above is treated by plasma, place it in a closed vacuum dryer. Through the volatile 1H,1H,2H,2H-perfluorodecyltrichlorosilane to form a fluorinated atmosphere in a vacuum-sealed environment, and let it stand for 6 h for surface fluorosilane modification; After the modification is completed, rinse the sample surface with anhydrous ethanol and dry it with nitrogen or compressed air to obtain a transparent super-hydrophobic and super-oleophobic coating.

[0022] Further, in the step S2, the standing time is 30 - 60 min.

[0023] The second technical solution proposed by the present invention is an amorphous photonic crystal microsphere prepared by the above method.

[0024] The third technical solution proposed by the present invention is the application of the amorphous photonic crystal microsphere in biological detection and the preparation of structural color pigments.

[0025] Compared with the prior art, the present invention first uses centrifugation operation to pre-form a poly(styrene-methyl methacrylate-acrylic acid) nano-microsphere emulsion for pre-crystallization, and then through evaporation-induced self-assembly on a super-hydrophobic and super-oleophobic surface, it can quickly form amorphous, highly spherical and uniform microspheres with short-range order and long-range disorder. The invention can be used to mass-produce amorphous photonic crystal microspheres with uniform structure, stable performance and simple components. Therefore, the materials used in the present invention are simple, the cost is low, the preparation process is simple and fast, the operability is strong, the preparation method is easy to implement, and it is easy to be used in industrial scale. Description of the Drawings

[0026] Figure 1Optical microscope image of the amorphous photonic crystal microspheres prepared in Example 1.

[0027] Figure 2 SEM image of the internal structure of the amorphous photonic crystal microspheres prepared in Example 1.

[0028] Figure 3 Reflection spectra of the amorphous photonic crystal microspheres prepared in Example 1 at different angles. Detailed implementation manners

[0029] To make the objectives, technical solutions and advantages of the present invention clearer and more understandable, the present invention will be further described in detail below in conjunction with embodiments. The specific embodiments described herein are only used to explain the present invention and are not used to limit the invention.

[0030] Unless otherwise specified, the raw materials and reagents used in the following examples are all commercially available.

[0031] In this embodiment, a transparent superhydrophobic and superoleophobic coating is first prepared, and the specific preparation process is as follows:

[0032] 1) Preparation of nano-sphere mixed silica sol: Mix absolute ethanol and a monodisperse polystyrene nano-sphere emulsion with a diameter of 50 nm and a mass concentration of 5% at a mass ratio of 5:1 to prepare solution A; Mix tetraethyl orthosilicate TEOS, 0.1 mol / L hydrochloric acid and absolute ethanol at a mass ratio of 1:1:1.5 to prepare solution B; Take 6 g of solution A and 0.2 g of solution B, mix them by oscillation, stir well with a magnetic stirrer for 40 min, and then ultrasonically disperse the formed partially hydrolyzed prepolymer solution for 15 min to prepare the nano-sphere mixed silica sol;

[0033] 2) Pretreatment of aluminum sheet: Clean the aluminum sheet, then soak it in an absolute ethanol solution and ultrasonicate for 30 min, then put the aluminum sheet into deionized water and ultrasonicate for 10 min, take it out and dry it with nitrogen or compressed air. The treated aluminum sheet is immersed in 1 mol / L hydrochloric acid for 1 min, taken out, washed clean with ultrapure water, and dried again with nitrogen or compressed air, and then treated by plasma for 5 min, taken out and kept away from dust for standby.

[0034] 3) Spraying: Place the pretreated aluminum sheet on a hot plate at 85 °C and heat for 5 min or until the surface temperature is stable, then use a spray gun to spray the prepared nano-sphere mixed silica sol on the surface of the aluminum sheet under the conditions of a set pressure of 0.40 MPa and a distance of 15 cm from the surface of the aluminum sheet to form a cured film;

[0035] 4) Calcination: Transfer the aluminum sheet with a cured film on the surface to a muffle furnace, heat it at a heating rate of 2 °C / min to 500 °C, keep it for 2 h, and then naturally cool to obtain an aluminum sheet with a transparent coating;

[0036] 5) Low surface energy modification: The aluminum sheet containing the transparent coating prepared above is treated with plasma and then placed in a sealed vacuum dryer. A fluorinated atmosphere is formed in a vacuum-sealed environment by volatile 1H, 1H, 2H, 2H-perfluorodecyltrichlorosilane, and the surface is left to stand for 6 hours for surface fluorosilane modification. After the modification is completed, the sample surface is rinsed with anhydrous ethanol and blown dry with nitrogen or compressed air to obtain a transparent super-amphiphobic coating for standby use.

[0037] Then, a monodispersed poly(styrene-methyl methacrylate-acrylic acid) nanosphere emulsion is prepared, and the specific preparation process is as follows:

[0038] 1) First, perform vacuum distillation of styrene to remove the polymerization inhibitor: add styrene and a 5% sodium hydroxide solution by mass into a volumetric flask, wash it upside down twice until it is colorless, let it stand for stratification, separate and remove the upper liquid with a separatory funnel, then wash it with ultrapure water until it is neutral, add 0.5% cuprous chloride during distillation, first heat it in a water bath at 60°C to remove the front distillate component, then heat it to 68°C to 75°C to collect the main distillate, and obtain styrene with the polymerization inhibitor removed, cool it at room temperature, and store it in a refrigerator for later use;

[0039] 2) Synthesis reaction: 2g of 1g / L emulsifier sodium dodecylbenzene sulfonate solution, 0.5g of ammonium bicarbonate solution and the remaining ultrapure water (80mL) were placed in a three-necked flask, heated in a water bath at 60°C and stirred thoroughly with a magnetic stirring paddle at a stirring speed of 350rpm; then 19g of redistilled styrene, 1g of methyl methacrylate and 1g of acrylic acid were added, the stirring speed was maintained at 350rpm and the temperature was raised to 60°C and maintained for 1h, and then the temperature was raised to 70°C and maintained for 30min; 12mL of initiator ammonium persulfate solution was slowly added at a rate of 3s / drop, and the temperature was stirred at a constant temperature for 1.5h; after the temperature was raised to 80°C, 4mL of APS solution was slowly added at the same rate, and the temperature was stirred for 2h; finally, 4mL of APS solution was slowly added again, and the reaction was continued for 2h; after the reaction was completed, the product was taken out, cooled and bottled to obtain a monodisperse poly(styrene-methyl methacrylate-acrylic acid) nanosphere emulsion for standby use.

[0040] Example 1

[0041] 1) 1 mL of monodispersed poly(styrene-methyl methacrylate-acrylic acid) nanosphere emulsion and 4 mL of deionized water were added to a 7 mL centrifuge tube, centrifuged at 8000 rpm for 60 min, and then the supernatant was removed. Ultrasonic vibration was performed to obtain an emulsion with structural color.

[0042] 2) Prepare amorphous poly(styrene-methyl methacrylate-acrylic acid) photonic crystal microspheres by the method of evaporation-induced self-assembly. Specifically, at 21 °C and 65% humidity, use a pipette to aspirate 10 μL of the emulsion with structural color, drop the liquid onto an aluminum sheet with a transparent super-hydrophobic and super-oleophobic coating, and let it stand for 60 min to obtain self-assembled amorphous photonic crystal microspheres.

[0043] Example 2

[0044] 1) Add 1 mL of poly(styrene-methyl methacrylate-acrylic acid) nano-microsphere emulsion and 4 mL of deionized water into a 7 mL centrifuge tube. After centrifuging at 8000 rpm for 60 min, remove the upper clear liquid, and ultrasonically oscillate it evenly to obtain an emulsion with structural color.

[0045] 2) Prepare amorphous poly(styrene-methyl methacrylate-acrylic acid) photonic crystal microspheres by the method of evaporation-induced self-assembly. Specifically, at 21 °C and 65% humidity, use a pipette to aspirate 10 μL of the emulsion with structural color, drop the liquid onto an aluminum sheet with a transparent super-hydrophobic and super-oleophobic coating, and let it stand for 30 min to obtain self-assembled amorphous photonic crystal microspheres.

[0046] Example 3

[0047] 1) Add 1 mL of poly(styrene-methyl methacrylate-acrylic acid) nano-microsphere emulsion and 4 mL of deionized water into a 7 mL centrifuge tube. After centrifuging at 8000 rpm for 60 min, remove the upper clear liquid, and ultrasonically oscillate it evenly to obtain an emulsion with structural color.

[0048] 2) Prepare amorphous poly(styrene-methyl methacrylate-acrylic acid) photonic crystal microspheres by the method of evaporation-induced self-assembly. Specifically, at 21 °C and 65% humidity, use a pipette to aspirate 10 μL of the emulsion with structural color, drop the liquid onto an aluminum sheet with a transparent super-hydrophobic and super-oleophobic coating, and let it stand for 45 min to obtain self-assembled amorphous photonic crystal microspheres.

[0049] Taking the amorphous photonic crystal microspheres prepared in Example 1 as an example, the optical microscope image of the amorphous photonic crystal microspheres is as Figure 1 shown, and the SEM image of the internal structure of the amorphous photonic crystal microspheres is as Figure 2 shown. From Figure 1 and Figure 2 it can be seen that: the amorphous photonic crystal microspheres have uniform sizes, and an all-symmetric structure that is infinitely close to a spherical shape, with a smooth, complete, and crack-free surface. The surface is periodically arranged by colloidal particles, while the internal structure is an amorphous arrangement with short-range order and long-range disorder.

[0050] The reflection spectra of the amorphous photonic crystal microspheres at different angles are as follows Figure 3 shown, and it can be seen from Figure 3 that the reflection spectral curves of the incident light from multiple angles are very close. At the same time, due to its structural characteristics, the propagation characteristics of light are optimized, and the peak reflectivity can exceed 70%.

[0051] The technical solution of the present invention is not limited to the limitations of the above specific embodiments. Any technical deformation made according to the technical solution of the present invention falls within the protection scope of the present invention.

Claims

1. A method for preparing amorphous photonic crystal microspheres using a super-biphobic surface, characterized in that, The following steps are involved: S1, mixing a poly(styrene-methyl methacrylate-acrylic acid) nanosphere emulsion and deionized water in a volume ratio of 1:4, removing the upper clear liquid after centrifugation, and performing ultrasonic oscillation on the lower emulsion to obtain an emulsion with structural color; S2. Under the conditions of temperature 21° C. and humidity 65%, the emulsion with structural color is dropped onto an aluminum sheet with a transparent super-amphiphobic coating, and the emulsion with structural color is allowed to self-assemble to form amorphous photonic crystal microspheres.

2. The method for preparing amorphous photonic crystal microspheres using a super-biphobic surface according to claim 1, wherein The preparation process of the poly(styrene-methyl methacrylate-acrylic acid) nanosphere emulsion is as follows: 1) First, perform vacuum distillation of styrene to remove the polymerization inhibitor: add styrene and a 5% sodium hydroxide solution by mass into a volumetric flask, wash it upside down twice until it is colorless, let it stand for stratification, separate and remove the upper liquid with a separatory funnel, then wash it with ultrapure water until it is neutral, add 0.5% cuprous chloride during distillation, first heat it in a water bath at 60°C to remove the front distillate component, then heat it to 68°C to 75°C to collect the main distillate, and obtain styrene with the polymerization inhibitor removed, cool it at room temperature, and store it in a refrigerator for later use; 2) Synthesis reaction: 2g of 1g / L emulsifier sodium dodecylbenzene sulfonate solution, 0.5g of ammonium bicarbonate solution and the remaining ultrapure water (80mL) were placed in a three-necked flask, heated in a water bath at 60°C and stirred thoroughly with a magnetic stirring paddle at a stirring speed of 350rpm; then 19g of redistilled styrene, 1g of methyl methacrylate and 1g of acrylic acid were added, the stirring speed was maintained at 350rpm and the temperature was raised to 60°C and maintained for 1h, and then the temperature was raised to 70°C and maintained for 30min; 12mL of initiator ammonium persulfate solution was slowly added at a rate of 3s / drop, and the temperature was stirred at a constant temperature for 1.5h; after the temperature was raised to 80°C, 4mL of APS solution was slowly added at the same rate, and the temperature was stirred for 2h; finally, 4mL of APS solution was slowly added again, and the reaction was continued for 2h; after the reaction was completed, the suspension was taken out, cooled and bottled to obtain a monodisperse poly(styrene-methyl methacrylate-acrylic acid) nanosphere suspension.

3. The method for preparing amorphous photonic crystal microspheres using a superhydrophobic and superoleophobic surface according to claim 1, characterized in that, In the step S1, the centrifugal speed is 8000 rpm and the centrifugal time is 60 min.

4. The method for preparing amorphous photonic crystal microspheres using a superhydrophobic and superoleophobic surface according to claim 1, characterized in that, In step S2, the preparation process of the transparent super-amphiphobic coating is as follows: 1) Preparation of nanosphere mixed silica sol: Anhydrous ethanol and a monodisperse polystyrene nanosphere emulsion with a diameter of 50 nm and a mass concentration of 5% were mixed in a mass ratio of 5:1 to prepare liquid A; tetraethyl silicate TEOS, 0.1 mol / L hydrochloric acid and anhydrous ethanol were mixed in a mass ratio of 1:1:1.5 to prepare liquid B; 6 g of liquid A and 0.2 g of liquid B were oscillated and mixed, and stirred for 40 minutes using a magnetic stirrer, and then the partially hydrolyzed prepolymer solution was ultrasonically dispersed for 15 minutes to prepare a nanosphere mixed silica sol; 2) Pretreatment of aluminum sheet: Clean the aluminum sheet, then soak it in anhydrous ethanol solution and ultrasonicate for 30 min, then put the aluminum sheet into deionized water and ultrasonicate for 10 min. After taking it out, dry it with nitrogen or compressed air. The treated aluminum sheet is immersed in 1 mol / L hydrochloric acid for 1 min, taken out and washed clean with ultrapure water. After drying again with nitrogen or compressed air, it is treated by plasma for 5 min, taken out and stored away from dust for standby; 3) Spraying: Place the pretreated aluminum sheet on a hot plate at 85 °C and heat for 5 min or until the surface temperature is stable. Under the conditions of setting the pressure of the spray gun at 0.40 MPa and the distance from the surface of the aluminum sheet at 15 cm, spray the prepared nanosphere mixed silica sol on the surface of the aluminum sheet to cure into a film; 4) Calcination: Transfer the aluminum sheet with a cured film on the surface to a muffle furnace, heat it at a heating rate of 2 °C / min to 500 °C and hold for 2 h, and then naturally cool to obtain an aluminum sheet with a transparent coating; 5) Low surface energy modification: After the aluminum sheet with a transparent coating prepared above is treated by plasma, place it in a closed vacuum dryer. Through the volatile 1H,1H,2H,2H-perfluorodecyltrichlorosilane to form a fluorination atmosphere in a vacuum-sealed environment, and let it stand for 6 h for surface fluorosilane modification; after the modification is completed, rinse the surface of the sample with anhydrous ethanol and dry it with nitrogen or compressed air to obtain a transparent superhydrophobic and superoleophobic coating.

5. The method for preparing amorphous photonic crystal microspheres using a superhydrophobic and superoleophobic surface according to claim 1, wherein In the step S2, the standing time is 30 - 60 min.

6. An amorphous photonic crystal microsphere prepared by the method according to any one of claims 1 - 5.

7. Use of the amorphous photonic crystal microsphere according to claim 6 in biological detection and preparation of structural color pigments.

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