Detection device and detection method for berberine hydrochloride

Abstract

The present invention provides a detection device and a detection method for berberine hydrochloride, which relate to the technical field of detection equipment. The device comprises a base, a chromatographic column oven and a detection box, wherein the chromatographic column oven and the detection box are electrically connected and fixedly mounted on the base, the chromatographic column oven is provided with a manual injector, and the detection box comprises a liquid storage tank tray, a degassing device, a detector and an infusion unit, which are sequentially installed from top to bottom, a control panel is installed on the side wall of the liquid storage tank tray, and a U-shaped rack is evenly and equidistantly fixedly mounted on the upper end of the liquid storage tank tray. The solvent bottle of the device can be kept in a horizontal position with the placement rack, and the static pressure of the mobile phase of the horizontally placed solvent bottle is constant. According to the principle of liquid statics, stable static pressure at the outlet means stable flow rate of the mobile phase, and the stable flow rate ensures consistent retention time of the sample in the chromatographic column, better peak separation, and more accurate quantitative results.

Description

Technical Field

[0001] The present invention belongs to the technical field of detection equipment, and more specifically, relates to a detection device and a detection method for berberine hydrochloride. Background Art

[0002] Berberine hydrochloride, as an important alkaloid, is widely found in various medicinal plants such as Coptis chinensis and Phellodendron amurense. It is widely used in the medical field. It has multiple pharmacological activities such as antibacterial, anti-inflammatory, antiarrhythmic, and hypoglycemic. It is used to treat various diseases such as intestinal infections and cardiovascular diseases. The accurate determination of its content is of vital importance for drug quality control, clinical efficacy evaluation, and related research. The content of berberine hydrochloride is closely related to the efficacy of the drug. Clinicians need to use liquid chromatography to measure the actual content of berberine hydrochloride in the drug and formulate a reasonable medication plan based on the patient's condition, weight, and other factors to achieve the best therapeutic effect.

[0003] However, the existing detection equipment for berberine hydrochloride still has the following deficiencies:

[0004] 1. When transporting or accidentally bumping in the laboratory, the solvent bottle is prone to shaking or tipping over, and the liquid level and flow state of the mobile phase in the bottle will change. This may make it easier for air to enter the infusion line, thereby generating bubbles. Once the bubbles enter the chromatographic system, they will interfere with the detection signal, causing baseline fluctuations, affecting the accuracy and repeatability of the analysis results. Moreover, if the bubbles accumulate in the pump, it may also cause pressure fluctuations in the pump, affecting the stable supply of mobile phase and reducing the separation effect.

[0005] 2. Cleaning solvent bottles with traditional equipment is cumbersome. The solvent bottles need to be fixed first and then cleaned individually, which consumes a lot of time and energy. Furthermore, the management of connecting tubes is chaotic, and there is a lack of effective storage and classification measures, resulting in a cluttered space around the instrument. This not only increases the risk of operational errors, but also shortens the service life of the connecting tubes due to excessive bending. Summary of the Invention

[0006] In order to solve the above technical problems, the present invention provides a detection device and a detection method for berberine hydrochloride to solve the above problems.

[0007] A detection device for berberine hydrochloride, comprising a base, a chromatographic column oven, and a detection box, wherein the chromatographic column oven and the detection box are electrically connected and fixedly mounted on the base, the chromatographic column oven is provided with a manual injector, and the detection box comprises a liquid storage tank tray, a degassing device, a detector, and an infusion unit, wherein the liquid storage tank tray, the degassing device, the detector, and the infusion unit are sequentially mounted from top to bottom, a control panel is mounted on the side wall of the liquid storage tank tray, a U-shaped frame is evenly and equidistantly fixedly mounted on the upper end of the liquid storage tank tray, and a first connecting ring is fixedly mounted on the lower end of each of the four U-shaped frames;

[0008] In which, a placement rack is provided inside the liquid storage tank tray, and a first slide groove is symmetrically opened on the upper end of the placement rack, and two groups of placement grooves are opened on the upper end of the placement rack, and the two groups of placement grooves are respectively connected with the two first slide grooves, and four second connecting rings are fixedly installed on the upper end of the placement rack, and inner rings are rotatably installed inside the four second connecting rings and the first connecting rings, and rolling balls are provided on the four groups of inner rings, and slides are slidably installed inside the two first slide grooves, and a driving motor is fixedly installed at the center of the upper end of the placement rack, and a screw is installed on the output end of the driving motor, and the screw is threadedly installed in the slide, and connecting ropes are connected between the four second connecting rings and the four first connecting rings.

[0009] Preferably, an inner groove is provided on the side wall of the liquid storage tank tray, and pipe clamps are equidistantly and slidably installed inside the inner groove.

[0010] Preferably, two groups of fixing rings are symmetrically fixedly installed on the slide, and the two groups of fixing rings are respectively arranged in the two groups of placement grooves.

[0011] Preferably, cleaning rings are fixedly installed inside the two groups of fixing rings, and a positioning frame is fixedly installed on the upper end of the slide.

[0012] Preferably, four positioning rings are fixedly mounted on the positioning frame, and limiting rings are fixedly mounted inside the four positioning rings.

[0013] Preferably, the four limiting rings are all equidistantly slidably installed with support columns, the four groups of support columns are all fixedly installed with first springs, and the ends of the four groups of first springs away from the four groups of support columns are fixedly connected to the four limiting rings respectively.

[0014] Preferably, storage racks are fixedly installed at equal distances on the four positioning rings, second slide grooves are opened inside the four groups of storage racks, third slide grooves are opened on the side walls of the four groups of storage racks, and the four groups of third slide grooves are respectively connected to the four groups of second slide grooves.

[0015] Preferably, the four groups of second chutes are all slidably installed with extension frames inside, the four groups of extension frames are all fixedly installed with sliders on the side walls, and the four groups of sliders are respectively slidably installed in the four groups of third chutes.

[0016] Preferably, the lower ends of the four groups of extension frames are all fixedly mounted with second springs, and the ends of the four groups of second springs away from the four groups of sliders are respectively fixedly mounted in the four groups of second sliding grooves.

[0017] A method for detecting berberine hydrochloride, comprising the following steps:

[0018] S1: First, install the solvent bottle on the placement rack, then use octadecylsilane bonded silica gel as the chromatographic column filler; use acetonitrile as mobile phase A and 0.1% phosphoric acid solution as mobile phase B. Add mobile phase A and mobile phase B to the solvent bottle and perform gradient elution. The elution program is as follows: 0-10 min, 15%→30%A; 11-20 min, 30%A→45%A; 21-30 min, 45%A→15%A; 31-40 min, 15%A; the detection wavelength is 265 nm; the flow rate is 1.0 mL / min; the column temperature is 30°C;

[0019] S2: Preparation of reference solution: Accurately weigh an appropriate amount of berberine hydrochloride reference substance and add methanol to prepare a berberine hydrochloride reference solution containing 1 mg of berberine hydrochloride per 1 ml.

[0020] S3: Preparation of test solution: Weigh an appropriate amount of the sample to be tested, add an appropriate amount of methanol and ultrasonically dissolve it for 10 minutes. After cooling, add methanol to make up the missing weight and filter to obtain the solution;

[0021] S4: Determination: Take the reference solution and the test solution, perform HPLC detection using the above-mentioned chromatographic conditions, and calculate the content of berberine hydrochloride in the test sample through the standard curve of berberine hydrochloride reference.

[0022] Compared with the prior art, the present invention has the following beneficial effects:

[0023] The first spring is stretched and the first spring generates an elastic force to make the post press against the bottle mouth part of the solvent bottle, further completing the fixation of the solvent bottle. This double fixing method greatly enhances the stability of the solvent bottle fixation, effectively preventing the solvent bottle from shaking, tipping over, etc. during use, thereby ensuring the safety and accuracy of the experiment or production process.

[0024] In the present invention, by providing a base, a detection box, a first connecting ring, a placement rack and a second connecting ring, when the solvent bottle is fixed, the two ends of the four connecting ropes are installed on the four groups of first connecting rings and second connecting rings, and the placement rack will be in a suspended state. When the detection box and the base are in the process of transportation or tilted due to human error, the solvent bottle will also tilt accordingly. At this time, under the action of gravity, the solvent bottle will be kept in a horizontal position along with the placement rack, the liquid level and flow state of the mobile phase in the bottle are relatively stable, and it is difficult for air to enter the infusion pipeline, thereby greatly reducing the possibility of bubble generation, and the static pressure of the mobile phase in the horizontally placed solvent bottle is constant. According to the principle of liquid statics, the stable static pressure at the outlet means the stable flow rate of the mobile phase. The stable flow rate ensures the consistent retention time of the sample in the chromatographic column, better peak separation, and more accurate quantitative results.

[0025] In the present invention, a fixing ring and a cleaning ring are provided. When the fixing ring moves, the cleaning ring is driven to move downward. At this time, the cleaning ring is squeezed and contacts a portion of the solvent bottle body. When the cleaning ring continues to move downward and fix, the solvent bottle body is cleaned. This design avoids the tedious steps of first fixing the solvent bottle and then cleaning it separately in traditional operations, and can effectively remove impurities such as dust, stains, and fingerprints on the surface of the bottle body. If these impurities are not removed in time, they may fall off and enter the solvent during the experiment, affecting the accuracy of the experimental results. In particular, in analytical experiments with high experimental precision requirements, a clean solvent bottle body can reduce the interference of impurities on the experiment and ensure the reliability of the experimental data.

[0026] In the present invention, a storage rack, a second slide groove, a third slide groove, an extension rack, a slider and a second spring are provided, so that the connecting tube of the solvent bottle is wound and stored by the storage rack. When the connecting tube is too long, the extension rack can be pulled upward. At this time, the extension rack and the slider slide upward in the second slide groove and the third slide groove respectively. At the same time, the second spring will be stretched. The storage amount of the connecting tube can be adjusted by adjusting the height of the extension rack. The storage amount is adjusted by using the extension rack to accurately adapt to connecting tubes of different lengths, meet various experimental needs, improve work efficiency, optimize the experimental space, reduce the safety hazards caused by the cluttered placement of connecting tubes, and avoid excessive bending of the connecting tubes, thereby extending the service life, adapting to different experimental scenarios, and enhancing the versatility of the equipment.

[0027] In the present invention, by providing an inner groove and a tube clamp, the free end of the tube can be passed through the inner groove and placed inside the tube clamp. At this time, different connecting tubes are classified by the tube clamp inside the inner groove. Through the design of the tube clamp, the connecting tubes are then connected to the pump for testing, which further enhances the anti-entanglement effect of the connecting tubes, effectively organizes and utilizes the space around the instrument, improves work efficiency, and reduces operational errors caused by cluttered space.

[0028] In the present invention, fewer reagents are used in the detection process, which saves detection costs. The mobile phase composition is simpler than in previous methods, which reduces the probability of error. The operation is simpler and the accuracy of the measurement results remains unchanged. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 It is a schematic diagram of the three-dimensional structure of the present invention;

[0030] Figure 2 It is a schematic diagram of the pipe clamp connection structure of the present invention;

[0031] Figure 3 This is a schematic diagram of the explosion structure of the placement rack connection of the present invention;

[0032] Figure 4 This is a schematic diagram of the fixed ring connection explosion structure of the present invention;

[0033] Figure 5 It is a schematic diagram of the carriage connection structure of the present invention;

[0034] Figure 6 This is a schematic diagram of the inner ring connection explosion structure of the present invention;

[0035] Figure 7 Schematic diagram of the connection structure of the limit ring of the present invention;

[0036] Figure 8 It is a schematic diagram of the connection structure of the storage rack of the present invention;

[0037] Figure 9It is a schematic diagram of the explosion structure of the extension frame connection of the present invention.

[0038] 18. Detection box; 19. Manual injector; 20. Liquid storage tank tray; 21. Degassing device; 22. Detector; 23. Inner groove; 24. Tube clamp; 25. Placement rack; 26. First slide groove; 27. Placement groove; 31. Second connecting ring; 32. Inner ring; 33. Rolling ball; 34. Slide; 35. Drive motor; 36. Screw; 37. Connecting rope; 38. Fixed ring; 39. Cleaning ring; 41. Positioning rack; 42. Positioning ring; 43. Limiting ring; 44. Pillar; 45. First spring; 46. Storage rack; 47. Second slide groove; 48. Third slide groove; 49. Extension rack; 51. Slider; 52. Second spring. DETAILED DESCRIPTION

[0039] The following embodiments of the present invention are described in further detail with reference to the accompanying drawings and examples. The following examples are used to illustrate the present invention but are not intended to limit the scope of the present invention.

[0040] See also Figures 1-9 The present invention provides a detection device for berberine hydrochloride, comprising a base 11, a chromatographic column oven 12 and a detection box 13. The chromatographic column oven 12 is used to separate samples. The chromatographic column oven 12 and the detection box 13 are electrically connected and fixedly installed on the base 11. A manual injector 14 is provided on the chromatographic column oven 12. The manual injector 14 is a device for sending samples into the chromatographic column. The detection box 13 comprises a liquid storage tank tray 15, a degassing device 16, a detector 17 and an infusion unit 18. The liquid storage tank tray 15, the degassing device 16, the detector 17 and the infusion unit 18 are installed in sequence from top to bottom. The tank tray 15 is used to store a sufficient amount of mobile phase that meets the requirements. It is equipped with a solvent filter to prevent particles in the mobile phase from entering the pump. The degasser 16 is used to prevent bubbles released when the mobile phase flows out of the chromatographic column and enter the detector 17. The infusion unit 18 is connected to the mobile phase in the reservoir and continuously enters the liquid system in a high-pressure form, so that the sample completes the separation process in the chromatographic column. A control panel 19 is installed on the side wall of the liquid storage tank tray 15. The detector 17 converts the sample components continuously flowing out of the chromatographic column into easily measurable electrical signals, which are received by the control panel 19 to obtain a chromatogram of the sample separation.

[0041] A U-shaped frame 21 is evenly and equidistantly fixed on the upper end of the liquid storage tank tray 15, and a first connecting ring 22 is fixedly installed on the lower end of the four U-shaped frames 21. A placement rack 25 is provided inside the liquid storage tank tray 15, and a first slide 26 is symmetrically opened on the upper end of the placement rack 25. Two groups of placement slots 27 are opened on the upper end of the placement rack 25, and the two groups of placement slots 27 are respectively connected to the two first slides 26. Four second connecting rings 31 are fixedly installed on the upper end of the placement rack 25, and inner rings 32 are rotatably installed inside the four second connecting rings 31 and the first connecting ring 22. Rolling balls 33 are provided on the four groups of inner rings 32, and slides 34 are slidably installed inside the two first slides 26. A driving motor 35 is fixedly installed at the center of the upper end of the placement rack 25, and a screw 36 is installed on the output end of the drive motor 35. The screw 36 is threadedly installed in the slide 34. The four second connecting rings 31 and the four first connecting rings 22 are fixedly installed. A connecting rope 37 is connected between each connecting ring 22. When the solvent bottle is fixed, the two ends of the four connecting ropes 37 are installed on the four groups of first connecting rings 22 and second connecting rings 31. At this time, the placement rack 25 will be in a suspended state. When the detection box 13 and the base 11 are in the process of transportation or tilted due to human error, the solvent bottle will also tilt accordingly. At this time, under the action of gravity, the solvent bottle will be kept in a horizontal position along with the placement rack 25. The liquid level and flow state of the mobile phase in the bottle are relatively stable, and it is difficult for air to enter the infusion pipeline, thereby greatly reducing the possibility of bubble generation. In addition, the static pressure of the mobile phase in the horizontally placed solvent bottle is constant. According to the principle of liquid statics, stable static pressure at the outlet means stable flow rate of the mobile phase. The stable flow rate ensures consistent retention time of the sample in the chromatographic column, better peak separation, and more accurate quantitative results.

[0042] An inner groove 23 is provided on the side wall of the liquid storage tank tray 15. A pipe clamp 24 is equidistantly slidably installed inside the inner groove 23. When the connecting pipe is entangled, its free end can be passed through the inner groove 23 and placed inside the pipe clamp 24. At this time, different connecting pipes are classified by the pipe clamp 24 inside the inner groove 23. Through the design of the pipe clamp 24, the connecting pipe can be connected to the pump for testing, which further enhances the anti-entanglement effect of the connecting pipe, effectively organizes and utilizes the space around the instrument, improves work efficiency, and reduces operational errors caused by cluttered space.

[0043] The cleaning ring 39 is fixedly mounted on the upper end of the slide 34 , and the cleaning ring 39 is fixedly mounted on the upper end of the slide 34 .

[0044] Four positioning rings 42 are fixedly installed on the positioning frame 41, and limit rings 43 are fixedly installed inside the four positioning rings 42. Columns 44 are equidistantly slidably installed inside the four limit rings 43. First springs 45 are fixedly installed on the four groups of columns 44. One end of the four groups of first springs 45 away from the four groups of columns 44 is fixedly connected to the four limit rings 43 respectively. When in use, the solvent bottle can be placed in the placement groove 27 first, and then the drive motor 35 can be controlled to start through the control panel 19. Under the action of the drive motor 35, the screw 36 will be driven to rotate accordingly, and the rotation of the screw 36 will drive the slide 34 to move downward. At this time, the slide 34 drives the positioning frame 41 and the positioning ring 42 to move downward. When the bottle is in the bottle, the bottom of the bottle will fit into the bottleneck of the solvent bottle and exert a certain downward squeezing force on the solvent bottle to initially limit and fix the solvent bottle. At the same time, the positioning ring 42 will also drive the limiting ring 43 to move downward. Under the movement of the limiting ring 43, the support column 44 will be subjected to the squeezing force. At this time, the support column 44 will slide outward inside the limiting ring 43, and the first spring 45 will be stretched. At this time, the first spring 45 will generate elastic force to make the support column 44 press against the bottle mouth of the solvent bottle, further completing the fixation of the solvent bottle. This double fixing method greatly enhances the stability of the solvent bottle fixation, effectively preventing the solvent bottle from shaking or tipping over during use, and ensuring the safety and accuracy of the experiment or production process.

[0045] Storage racks 46 are fixedly installed at equal distances on the four positioning rings 42, and second slide grooves 47 are provided inside the four groups of storage racks 46. Third slide grooves 48 are provided on the side walls of the four groups of storage racks 46. The four groups of third slide grooves 48 are respectively connected to the four groups of second slide grooves 47. Extension racks 49 are slidably installed inside the four groups of second slide grooves 47. Slide blocks 51 are fixedly installed on the side walls of the four groups of extension racks 49. The four groups of slide blocks 51 are respectively slidably installed in the four groups of third slide grooves 48. Second springs 52 are fixedly installed on the lower ends of the four groups of extension racks 49. The ends of the four groups of second springs 52 away from the four groups of slide blocks 51 are respectively fixed in the four groups of second slide grooves 47. When the connecting tube on the solvent bottle is too long, the connecting tube can be wrapped around it. On the storage rack 46, the connecting tube of the solvent bottle is wrapped and stored through the storage rack 46. When the connecting tube is too long, the extension rack 49 can be pulled upward. At this time, the extension rack 49 and the slider 51 slide upward inside the second slide groove 47 and the third slide groove 48 respectively. At the same time, the second spring 52 will be stretched. The storage amount of the connecting tube is adjusted by adjusting the height of the extension rack 49. The storage amount is adjusted by using the extension rack 49 to accurately adapt to connecting tubes of different lengths, meet various experimental needs, improve work efficiency, optimize the experimental space, reduce the safety hazards caused by the messy placement of connecting tubes, and avoid excessive bending of the connecting tubes, extend the service life, adapt to different experimental scenarios, and enhance the versatility of the equipment.

[0046] A method for detecting berberine hydrochloride, comprising the following steps:

[0047] S1: First, install the solvent bottle on the placement rack 25, then use octadecylsilane bonded silica gel as the chromatographic column filler; use acetonitrile as mobile phase A and 0.1% phosphoric acid solution as mobile phase B. Add mobile phase A and mobile phase B to the solvent bottle and perform gradient elution. The elution program is as follows: 0-10 min, 15% → 30% A; 11-20 min, 30% A → 45% A; 21-30 min, 45% A → 15% A; 31-40 min, 15% A; the detection wavelength is 265 nm; the flow rate is 1.0 mL / min; the column temperature is 30°C;

[0048] S2: Preparation of reference solution: Accurately weigh an appropriate amount of berberine hydrochloride reference substance and add methanol to prepare a berberine hydrochloride reference solution containing 1 mg of berberine hydrochloride per 1 ml.

[0049] S3: Preparation of test solution: Weigh an appropriate amount of the sample to be tested, add an appropriate amount of methanol and ultrasonically dissolve it for 10 minutes. After cooling, add methanol to make up the missing weight and filter to obtain the solution;

[0050] S4: Determination: Take the reference solution and the test solution, perform HPLC detection using the above-mentioned chromatographic conditions, and calculate the content of berberine hydrochloride in the test sample through the standard curve of berberine hydrochloride reference.

[0051] Working principle:

[0052] In the first step, when using, the solvent bottle can be placed in the placement groove 27, and then the drive motor 35 can be controlled to start through the control panel 19. Under the action of the drive motor 35, the screw 36 will be driven to rotate, and the rotation of the screw 36 will drive the slide 34 to move downward. At this time, the slide 34 drives the positioning frame 41 and the positioning ring 42 to move downward. Under the movement of the positioning ring 42, its bottom will fit with the bottleneck of the solvent bottle and exert a certain downward squeezing force on the solvent bottle to initially limit and fix the solvent bottle. At the same time, the positioning ring 42 The stop ring 43 will also be driven to move downward. As the stop ring 43 moves, the support column 44 will be subjected to the extrusion force. At this time, the support column 44 will slide outward inside the stop ring 43, and the first spring 45 will be stretched. At this time, the first spring 45 will generate elastic force to make the support column 44 press against the bottle mouth of the solvent bottle, further completing the fixation of the solvent bottle. This double fixation method greatly enhances the stability of the solvent bottle fixation, effectively preventing the solvent bottle from shaking or tipping over during use, and ensuring the safety and accuracy of the experiment or production process.

[0053] In the second step, after the solvent bottle is fixed, the two ends of the four connecting ropes 37 are installed on the four groups of first connecting rings 22 and second connecting rings 31. At this time, the placement rack 25 will be in a suspended state. When the detection box 13 and the base 11 are in the process of transportation or tilted due to human error, the solvent bottle will also tilt accordingly. At this time, under the action of gravity, the solvent bottle will be kept in a horizontal position along with the placement rack 25. The liquid level and flow state of the mobile phase in the bottle are relatively stable, and it is difficult for air to enter the infusion line, thereby greatly reducing the possibility of bubble generation. In addition, the static pressure of the mobile phase in the horizontally placed solvent bottle is constant. According to the principle of liquid statics, the stable static pressure at the outlet means the stable flow rate of the mobile phase. The stable flow rate ensures the consistent retention time of the sample in the chromatographic column, better peak separation, and more accurate quantitative results.

[0054] In the third step, when the equipment is fixing the solvent bottle, the positioning frame 41 will synchronously drive the fixing ring 38 to move downward, and the movement of the fixing ring 38 will drive the cleaning ring 39 to move downward accordingly. At this time, the cleaning ring 39 will be squeezed and contact the body of the solvent bottle. When it continues to move downward and fix, the body of the solvent bottle will be cleaned. This design avoids the tedious steps of fixing the solvent bottle first and then cleaning it separately in traditional operations, and can effectively remove impurities such as dust, stains, fingerprints, etc. on the surface of the bottle. If these impurities are not removed in time, they may fall off and enter the solvent during the experiment, affecting the accuracy of the experimental results. Especially in analytical experiments with high experimental precision requirements, a clean solvent bottle body can reduce the interference of impurities on the experiment and ensure the reliability of the experimental data.

[0055] In the fourth step, when the connecting tube on the solvent bottle is too long, the connecting tube can be wrapped around the storage rack 46, and the connecting tube of the solvent bottle can be wrapped and stored through the storage rack 46. When the connecting tube is too long, the extension rack 49 can be pulled upward. At this time, the extension rack 49 and the slider 51 slide upward inside the second slide groove 47 and the third slide groove 48 respectively. At the same time, the second spring 52 will be stretched. The storage amount of the connecting tube is adjusted by adjusting the height of the extension rack 49. The storage amount is adjusted by using the extension rack 49 to accurately adapt to connecting tubes of different lengths, meet various experimental needs, improve work efficiency, optimize the experimental space, reduce the safety hazards caused by the cluttered placement of connecting tubes, and avoid excessive bending of the connecting tubes, extend the service life, adapt to different experimental scenarios, and enhance the versatility of the equipment.

[0056] In the fifth step, after the connecting tube is entangled, its free end can be passed through the inner groove 23 and placed inside the tube clamp 24. At this time, the different connecting tubes are classified by the tube clamp 24 inside the inner groove 23. Through the design of the tube clamp 24, the connecting tube is then connected to the pump for testing, which further enhances the anti-entanglement effect of the connecting tube, effectively organizes and utilizes the space around the instrument, improves work efficiency, and reduces operational errors caused by cluttered space.

[0057] In the sixth step, after the solvent bottle is fixed, the sample is injected through the manual injector 14, and then the sample is tested through the chromatographic column oven 12 and the detection box 13.

[0058] The embodiments of the present invention are presented for purposes of illustration and description and are not intended to be exhaustive or to limit the invention to the disclosed forms. Many modifications and variations will be apparent to those skilled in the art. The embodiments are chosen and described in order to better illustrate the principles of the invention and its practical application and to enable those skilled in the art to understand the invention and design various embodiments with various modifications as suited for specific applications.

Claims

1. A detection device for berberine hydrochloride, comprising a base (11), a chromatographic column oven (12) and a detection box (13), wherein the chromatographic column oven (12) and the detection box (13) are electrically connected and fixedly mounted on the base (11), characterized in that: The chromatographic column oven (12) is provided with a manual injector (14), and the detection box (13) includes a liquid storage tank tray (15), a degassing device (16), a detector (17) and an infusion unit (18), wherein the liquid storage tank tray (15), the degassing device (16), the detector (17) and the infusion unit (18) are sequentially installed from top to bottom, and a control panel (19) is installed on the side wall of the liquid storage tank tray (15), and a U-shaped frame (21) is fixedly installed at an evenly spaced interval on the upper end of the liquid storage tank tray (15), and a first connecting ring (22) is fixedly installed on the lower end of each of the four U-shaped frames (21); The liquid storage tank tray (15) is provided with a placement rack (25) inside, and the upper end of the placement rack (25) is symmetrically provided with a first slide groove (26), and the upper end of the placement rack (25) is provided with two groups of placement grooves (27), and the two groups of placement grooves (27) are respectively connected to the two first slide grooves (26), and the upper end of the placement rack (25) is fixed with four second connecting rings (31), and the four second connecting rings (31) and the first connecting ring (22) are rotatably provided with inner rings (32), and the four groups of inner rings (32) are provided with rolling balls (33), and the two first slide grooves (26) are slidable inside. A slide (34) is installed, a driving motor (35) is fixedly installed at the center of the upper end of the placement frame (25), a screw (36) is installed on the output end of the driving motor (35), and the screw (36) is threadedly installed in the slide (34), and connecting ropes (37) are connected between the four second connecting rings (31) and the four first connecting rings (22), and the two ends of the four connecting ropes (37) are installed on the four groups of first connecting rings (22) and second connecting rings (31), and a positioning frame (41) is fixedly installed on the upper end of the slide (34), and four positioning rings (42) are fixedly installed on the positioning frame (41).

2. A detection device for berberine hydrochloride according to claim 1, characterized in that: An inner groove (23) is provided on the side wall of the liquid storage tank tray (15); Wherein, a pipe clamp (24) is equidistantly slidably mounted inside the inner groove (23).

3. A detection device for berberine hydrochloride according to claim 1, characterized in that: Two sets of fixing rings (38) are symmetrically fixedly mounted on the slide (34); The two groups of fixing rings (38) are respectively disposed in the two groups of placement grooves (27).

4. A detection device for berberine hydrochloride according to claim 3, characterized in that: Cleaning rings (39) are fixedly installed inside the two sets of fixing rings (38).

5. A detection device for berberine hydrochloride according to claim 1, characterized in that: A limiting ring (43) is fixedly installed inside each of the four positioning rings (42).

6. A detection device for berberine hydrochloride according to claim 5, characterized in that: Abutment posts (44) are equidistantly and slidably mounted inside the four limiting rings (43); The four groups of support columns (44) are all fixedly mounted with first springs (45), and one end of the four groups of first springs (45) away from the four groups of support columns (44) is fixedly connected to four limiting rings (43) respectively.

7. A detection device for berberine hydrochloride according to claim 5, characterized in that: Storage racks (46) are fixedly mounted on the four positioning rings (42) at equal distances, and second slide grooves (47) are provided inside the four groups of storage racks (46); The side walls of the four groups of storage racks (46) are each provided with a third sliding groove (48), and the four groups of third sliding grooves (48) are respectively connected to the four groups of second sliding grooves (47).

8. A detection device for berberine hydrochloride according to claim 7, characterized in that: Extension frames (49) are slidably mounted inside the four groups of the second chute (47); The four groups of extension frames (49) are all fixedly mounted with sliders (51) on their side walls, and the four groups of sliders (51) are respectively slidably mounted in the four groups of third slide grooves (48).

9. A detection device for berberine hydrochloride according to claim 8, characterized in that: The lower ends of the four groups of extension frames (49) are all fixedly mounted with second springs (52); Wherein, one end of the four groups of second springs (52) away from the four groups of sliders (51) is fixedly mounted in the four groups of second sliding grooves (47).

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