Control method of sample analyzer and sample analyzer

Abstract

The embodiment of the invention provides a control method of a sample analyzer and the sample analyzer, and the control method of the sample analyzer comprises the following steps: controlling a light source assembly of the sample analyzer to irradiate a reaction cup with a first optical signal, the first optical signal being an optical signal of a mixed wavelength; collecting third optical signals with at least two wavelengths formed by emitting the first optical signals through the reaction cup in parallel, wherein the third optical signals are obtained by splitting second optical signals formed by emitting the first optical signals through the reaction cup; monitoring the state of the liquid in the reaction cup or the reaction cup according to the at least two third optical signals; or acquiring the detection result of the sample added in the reaction cup according to the at least two third optical signals; or correcting the third optical signal of the second wavelength by adopting the third optical signal of the first wavelength in the at least two third optical signals.

Description

Technical Field

[0001] This application relates to the field of medical devices, and in particular, to a control method and a sample analyzer for a sample analyzer. Background Art

[0002] A sample analyzer is a device for measuring and analyzing samples. Typically, sample analyzers include biochemical analyzers, immunoassays, coagulation analyzers, etc. Among them, in a sample analyzer, the basic principle of detecting the concentration of an analyte by colorimetry is to obtain detection signals by periodically measuring the reaction solution during the reaction process of the reactants, obtain a reaction curve based on the detection signals, and obtain the measurement result of the corresponding analyte based on information such as the amplitude and change rate of the curve. Usually, during the detection process of the test solution in a reaction cup, the reaction cup will pass through the detector regularly, and multiple groups of optoelectronic signals at fixed intervals are collected (such as Figure 1 shown), and the sample analyzer processes the multiple groups of signals obtained periodically, so as to realize continuous, automatic and high-speed detection of a large number of samples.

[0003] Correspondingly, a general biochemical analyzer has multiple detection wavelengths, such as 8, 12, or 16, etc., as Figure 2 shown. During the measurement process of each wavelength, its corresponding absorbance reaction curve can be obtained, and the instrument calculates the result using one or several of the measurement results of multiple wavelengths according to needs.

[0004] Figure 2 The absorbances measured at different wavelengths corresponding to the same light collection point in are obtained when the reaction cup passes through a photometer once. The results of multiple wavelengths measured when the reaction cup passes through a photometer once are not measured simultaneously, but the time when the reaction cup passes through the photometer this time is segmented according to the number of wavelengths, and the signals of a specific wavelength are collected in each period of time, as Figure 3 shown. The figure shows that the reaction solution in the reaction cup collects signals of different wavelengths at different times, and a small square represents one wavelength. The problem with this prior art is that the measurement signals of multiple wavelengths at the same moment or in the same time period cannot be obtained simultaneously, and abnormal processing cannot be performed by comparing the signals of multiple wavelengths at the same moment or in the same time period, which easily produces abnormal results.

[0005] The corresponding acquisition circuit block diagram of this multi-wavelength optoelectronic data acquisition method is as Figure 6 shown. In this acquisition circuit, multiple-channel signal conditioning requires signal channel selection, and one signal is selected to enter analog-to-digital conversion. Due to the large amount of optoelectronic data, the prior art uses this relatively low-cost circuit to achieve multi-wavelength detection.

[0006] In addition, since the reaction cups and reactants are non-uniform, to ensure the measurement accuracy of multiple collection points, it is necessary to precisely control the relative light collection position of the photometer during multi-point absorbance measurement. In the photoelectric collection system of existing products, a photoelectric code disk with the same number of teeth as the number of reaction cups is installed under the reaction disk. Through assembly and debugging, the signal transition edge of each code tooth is aligned with the center of the corresponding reaction cup. When each code tooth transition 101 occurs due to the rotation of the reaction disk, signal collection is immediately started within the 102 interval, so as to obtain a reaction curve with smaller error and higher accuracy at a position with higher repeat accuracy of the reaction cup. Specifically as Figure 5 shown, since the top platform part of the photoelectric signal is relatively stable, it is usually necessary to collect the signal in the middle of the platform. Therefore, the signal edge of the code teeth of the photoelectric code disk needs to be precisely aligned with the center of each reaction cup, which makes the part processing and assembly debugging difficult. In addition, the hardware cost is also increased.

[0007] Therefore, the prior art needs to develop an optical data collection method with better multi-wavelength performance. Summary of the Invention

[0008] The main purpose of the embodiments of the present application is to provide a sample analyzer and a photoelectric data collection method for its reaction process, which can collect photoelectric data of multiple wavelengths in parallel at the same time, obtain more information, and facilitate subsequent data analysis and utilization.

[0009] In a first aspect, the embodiments of the present application provide a control method for a sample analyzer, including:

[0010] Controlling the light source component of the sample analyzer to irradiate the reaction cup with a first light signal, where the first light signal is a mixed-wavelength light signal;

[0011] Parallelly collecting at least two wavelengths of a third light signal formed by the first light signal after passing through the reaction cup, where the third light signal is obtained by splitting the second light signal after the first light signal passes through the reaction cup;

[0012] Monitoring the state of the liquid or the reaction cup in the reaction cup according to at least two third light signals;

[0013] Or, obtaining the detection result of the sample added to the reaction cup according to at least two of the third light signals.

[0014] In a second aspect, the embodiments of the present application provide a control method for a sample analyzer, including:

[0015] Controlling the light source component of the sample analyzer to irradiate the reaction cup with a first light signal, where the first light signal is a mixed-wavelength light signal;

[0016] Parallelly collect third optical signals of at least two wavelengths, where the third optical signals are obtained by splitting the second optical signal after the first optical signal exits from the reaction cup;

[0017] Use the third optical signal of the first wavelength among at least two third optical signals to correct the third optical signal of the second wavelength.

[0018] In a third aspect, an embodiment of the present application provides a sample analyzer, which is characterized by including a sample dispensing device, a reagent dispensing device, a reaction device, a detection device, and a controller;

[0019] The sample dispensing device is used to aspirate a sample from a sample container scheduled to the sampling position and transport the aspirated sample to a reaction cup;

[0020] The reagent dispensing device is used to dispense a reagent into the reaction cup;

[0021] The reaction device is used to incubate the reaction solution in the reaction cup, and the reaction solution is prepared from the reagent and the sample;

[0022] The detection device includes a light source assembly and an optical signal acquisition assembly. The light source assembly is used to emit a first optical signal to the reaction cup. The optical signal acquisition device includes a splitting device and at least two optical signal acquisition channels. The splitting device splits the second optical signal after the first optical signal exits from the reaction cup into third optical signals of at least two wavelengths. At least two of the optical signal acquisition assemblies parallelly collect the third optical signals, and the wavelengths of the third optical signals collected by different optical signal acquisition assemblies are different;

[0023] The controller is used to process the third optical signal to obtain a processing result. Description of the Drawings

[0024] To more clearly illustrate the technical solutions of the embodiments of the present application, the following will briefly introduce the drawings required for the description of the embodiments. Obviously, the following drawings are some embodiments of the present application. For those of ordinary skill in the art, without creative efforts, other drawings can also be obtained based on these drawings.

[0025] Figure 1 It is a reaction curve of two wavelengths plotted from the absorbances at different acquisition points obtained by collecting a reaction cup multiple times through a photometer. The abscissa represents time, the ordinate represents absorbance, Priwave represents the main wavelength, SecWave represents the secondary wavelength, and Pri - Sec represents the main wavelength minus the secondary wavelength;

[0026] Figure 2 It is a schematic diagram of a reaction curve of four wavelengths detected during the sample reaction process;

[0027] Figure 3 is a schematic diagram of an embodiment of a sample analyzer provided by an embodiment of the present application;

[0028] Figure 4 is a schematic diagram of the detection optical path of the sample analyzer provided by an embodiment of the present application

[0029] Figure 5 is a schematic diagram of the photometric analyzer collecting optoelectronic data of multiple wavelengths in time-division segments;

[0030] Figure 6 is a circuit block diagram of an optical signal acquisition component that collects optoelectronic data of multiple wavelengths in time-division segments;

[0031] Figure 7 is a schematic diagram of the code disk trigger signal detection;

[0032] Figure 8 is a schematic diagram of parallel acquisition of multi-wavelength optical data provided by an embodiment of the present application;

[0033] Figure 9 is a circuit block diagram of a photometer that can parallelly acquire optoelectronic data of multiple wavelengths provided by an embodiment of the present application;

[0034] Figure 10 is a schematic diagram of a pulse waveform for parallelly acquiring optoelectronic data of multiple wavelengths provided by an embodiment of the present application;

[0035] Figure 11 is a schematic diagram of an embodiment of a control method for the sample analyzer provided by the present application. Detailed implementation manners

[0036] Next, the technical solutions in the embodiments of the present application will be clearly and completely described in conjunction with the accompanying drawings in the embodiments of the present application. Obviously, the described embodiments are some, but not all, of the embodiments of the present application. All other embodiments obtained by those of ordinary skill in the art based on the embodiments in the present application without creative efforts shall fall within the protection scope of the present application.

[0037] In the description of the present application, unless otherwise clearly defined and limited, the terms "installation", "connection", and "connection" shall be understood in a broad sense. For example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection or an electrical connection; it may be a direct connection or an indirect connection through an intermediate medium, and it may be the communication inside two components. For those of ordinary skill in the art, the specific meanings of the above terms in the present application can be understood according to specific situations.

[0038] It should be understood that the terms used in the specification of this application are merely for the purpose of describing specific embodiments and are not intended to limit this application. As used in the specification of this application and the appended claims, unless the context clearly indicates otherwise, the singular forms "a", "an" and "the" are intended to include the plural forms.

[0039] Referring to Figure 1 , Figure 1 is a reaction curve of two wavelengths plotted from the absorbances at different collection points obtained by a reaction cuvette passing through a photometer multiple times. The abscissa represents time, and the ordinate represents absorbance. Priwave represents the primary wavelength, SecWave represents the secondary wavelength, and Pri-Sec represents the primary wavelength minus the secondary wavelength. Correspondingly, a biochemical analyzer has multiple detection wavelengths, such as 8, 12, or 16, etc., as Figure 2 shown. During the measurement of each wavelength, its corresponding absorbance reaction curve can be obtained, and the instrument calculates the result using one or several of the measurement results of multiple wavelengths as needed.

[0040] Figure 2 The absorbances measured at different wavelengths corresponding to the same light collection point in

[0041] Figure 3 is a schematic block diagram of a sample analyzer 1 according to an embodiment of this application. It includes a sample dispensing device 10, a reagent dispensing device 20, a reaction device 30, a detection device 40, and a controller 50, where:

[0042] The sample dispensing device 10 is used to aspirate a sample from a sample container scheduled to a sampling position and transport the aspirated sample to a reaction cuvette;

[0043] The reagent dispensing device 20 is used to dispense a reagent into the reaction cuvette;

[0044] The reaction device 30 is used to incubate the reaction solution in the reaction cuvette, and the reaction solution is prepared from the reagent and the sample;

[0045] The detection device 40, the detection device includes a light source assembly and a light signal collection assembly. The light source assembly is used to emit a first light signal to the reaction cuvette. The light signal collection device includes a spectroscope and at least two light signal collection channels. The spectroscope splits the second light signal exiting the reaction cuvette from the first light signal into third light signals of at least two wavelengths, and at least two of the light signal collection assemblies collect the third light signals in parallel, and the wavelengths of the third light signals collected by different light signal collection assemblies are different;

[0046] The controller 50 is used to process the third optical signal to obtain a processing result.

[0047] The controller 50 can be a Central Processing Unit (CPU). This processor can also be other general-purpose processors, Digital Signal Processors (DSPs), Application Specific Integrated Circuits (ASICs), Field-Programmable Gate Arrays (FPGAs), or other programmable logic devices, discrete gate or transistor logic devices, discrete hardware components, etc. Among them, the general-purpose processor can be a microprocessor or any conventional processor, etc.

[0048] The sample analyzer 1 may further include a memory. The memory can be a volatile memory, such as a Random Access Memory (RAM); or a non-volatile memory, such as a Read Only Memory (ROM), a flash memory, a Hard Disk Drive (HDD), or a Solid-State Drive (SSD); or a combination of the above types of memories. The memory is used to store computer programs and can provide instructions and data to the processor.

[0049] In one embodiment, the detection device 40 collects the full-time optoelectronic data (third optical signal) of multiple wavelengths when the reaction cup passes through multiple light collection points, and obtains a Figure 11 pulse waveform of multiple wavelengths as shown. By comparing the waveforms at multiple collection points, if abnormal conditions such as peaks or valleys appear at the top of the waveform, and if the pulse waveforms of all waveforms at the same moment of the reaction cup show the same abnormality, it indicates that there may be foreign objects or scratches in the reaction cup. It is also possible to further analyze the changes at the front and back light collection points to determine whether the foreign object in the reaction cup is a bubble or a solid. When the control device receives the prompt, it controls the instrument for maintenance or sends an alarm message to the user, or automatically arranges a retest.

[0050] Alternatively, if the signal sequences formed by the third optical signals of each processor are in the form of pulse waveforms, and abnormalities such as peaks or valleys are found in the pulse waveforms of several wavelengths near a certain wavelength spectrum (such as the main wavelength), while the same abnormalities do not occur at the same positions in the pulse waveforms of other wavelengths, it is indicated that the substance to be measured in the reaction solution may be uneven. When the control device receives the prompt, it controls the instrument for maintenance or sends a message indicating that the liquid in the reaction cup (the liquid in the reaction cup can be a reagent or a reaction solution) is uneven to the user, or automatically arranges a retest.

[0051] It can be understood that whether an abnormality occurs can be determined by the number of peaks or valleys of the third optical signal in multiple rotation cycles of the same reaction cup. For example, if the number of peaks or valleys of the same reaction cup in multiple rotation cycles is greater than a preset threshold, it can be considered that the reaction cup or the liquid in the reaction cup is abnormal; or, if the difference information of the third optical signals of different rotation cycles (optionally adjacent rotation cycles) corresponding to the same reaction cup exceeds the preset difference information, it is considered that the reaction cup or the liquid in the reaction cup is abnormal. The difference information may include the start time of collecting the reaction cup signal (i.e., the third optical signal), the rising edge duration of the third optical signal, the width of the waveform corresponding to the third optical signal, the falling edge duration of the third optical signal, and / or the end time of collecting the reaction cup signal (i.e., the third optical signal); in this embodiment, the abnormality of the reaction cup may be that the reaction cup is dirty or has scratches.

[0052] It can be understood that the liquid in the reaction cup can be not only a reagent, a sample or a reaction solution, but also a cleaning solution during the cleaning process of the reaction cup, and the abnormality during the cleaning process of the reaction cup is detected.

[0053] For the case of multiple wavelengths, the third optical signal for which the status detection can be selected in each rotation cycle, that is, each rotation cycle corresponds to a main wavelength, and the third optical signals corresponding to the main wavelengths of different cycles are compared, and whether it is abnormal is determined according to the comparison result. The third optical signals of different rotation cycles, the main wavelengths of different rotation cycles can be the same or different; or, the third optical signals of the same wavelength in different cycles are compared, and whether an abnormality occurs is determined according to the comparison result.

[0054] In another embodiment, it is also possible to compare the third optical signals of different wavelengths within the same rotation cycle of the reaction cup, and determine whether it is abnormal according to the comparison result. For example, the difference information of the third optical signals of different wavelengths within the same cycle exceeds the preset difference information; or, the number of third optical signals with the number of peaks or valleys greater than the preset threshold within the same cycle is greater than the preset number.

[0055] In a preferred embodiment, the detection device continuously and parallelly collects continuous pulse waveforms of multiple wavelengths, continuously stores these pulse waveforms, obtains the full-time optical signal changes of the reaction cup and / or the reaction solution, and determines abnormalities during the entire process of sample detection based on the changes in the optical signals.

[0056] In some embodiments, the light source assembly may include a point light source or a linear array light source formed by arranging multiple point light sources along the longitudinal axis of the reaction cup. When the linear array light source moves relative to the transverse axis of the reaction cup, the collected signal reflects the two-dimensional multi-wavelength signals of the reaction cup and / or the reactants, with richer information. By analyzing these data, the situation of the reaction process can be better grasped, and more abnormalities can be identified. It can be understood that in addition to the light source, the light source assembly may further include an optical component that changes the light transmission path to make the emitted light of the light source irradiate the reaction cup.

[0057] In this embodiment, the optical path between the light source assembly 11 and the optical signal acquisition assembly 14 is as Figure 4 shown. The light source assembly 11 irradiates the reaction cup with a first optical signal 21 of mixed wavelengths (such as white light). The first optical signal 21 forms a second optical signal 22 after being transmitted and / or scattered by the reaction cup 12. The second optical signal 22 is split by the splitting device 13 to form third optical signals 23 of multiple wavelengths. The reaction cup 12 or the liquid in the reaction cup 12 can be monitored through the third optical signals 23. Alternatively, the detection result of the sample added to the reaction cup 12 can also be obtained through the third optical signals 23. For example, absorbance or transmittance can be obtained through the third optical signals, and the detection result can be obtained based on the absorbance. It can be understood that the light source assembly 11 may include a single light source that emits white light or an array light source that emits optical signals of different wavelengths. The positions of the individual point light sources in the array light source irradiated in the reaction cup are the same, so that the incident optical signal of the reaction cup 12 is still the first optical signal 21 including optical signals of multiple different wavelengths.

[0058] In the technical solution disclosed in this embodiment, the second optical signal emitted from the reaction cup is split to form at least two third optical signals of different wavelengths. Through the third optical signals of different wavelengths emitted from the same position, subsequent correction can be performed or the detection result can be obtained based on the measurement signals at the same position.

[0059] In some embodiments, the reaction device includes a reaction disk, a driving device, and at least two reaction positions for placing reaction cups. The driving device drives the reaction disk to rotate so that the reaction cups move relative to the light source; it further includes a reaction driving assembly, and the driving assembly is used to drive the relative movement between the reaction cups and the light source assembly. The third optical signal is a signal sequence formed by the relationship between the light intensity and the acquisition time. The third optical signal is collected when the reaction cups pass by the light source during each rotation cycle of the reaction disk of the sample analyzer; the third optical signal is a signal sequence formed by the relationship between the light intensity and the acquisition time. The third optical signal is collected when the reaction cups pass by the light source during each rotation cycle of the reaction disk of the sample analyzer. The optical signals of the entire process of multiple wavelengths are collected in parallel. When the reaction cups pass by the light source assembly once, the time when the reaction cups pass by the light source assembly this time is not segmented according to the number of wavelengths, and the signal of a specific wavelength is collected for each period of time. Instead, the third optical signals of multiple wavelengths are collected in parallel at the same time. The detection positions and time periods of multiple wavelengths are the same. The advantage of this solution within a certain period of time is that the signals measured at the same position at the same time for multiple wavelengths can be obtained simultaneously. Subsequently, the measurement signals at the same position can be used for calibration or the detection results can be obtained.

[0060] To achieve parallel acquisition of multiple wavelengths, the optical signal acquisition component includes at least a photoelectric sensor and a signal processor. The signal processor is used to process the electrical signals output by the photoelectric sensor, and the corresponding signal acquisition circuit block diagram is as Figure 9 shown. In this embodiment, the selection of the optical signal acquisition channel is omitted. Each wavelength signal channel has an independent signal acquisition channel, and each independent signal acquisition channel has a corresponding photoelectric sensor and a corresponding analog-to-digital conversion function. Those skilled in the art can understand that one analog-to-digital conversion device can correspond to each wavelength signal channel, or multiple signal channels can correspond to one analog device, as long as the signals of each wavelength signal channel can be independently subjected to analog-to-digital conversion. Since each wavelength signal channel has its own analog-to-digital conversion ability, the optoelectronic data of multiple wavelengths can be collected at the same moment. Further, during the relative movement between the reaction cups and the light source, the optoelectronic data of multiple wavelengths at multiple moments (positions) are continuously collected, which can greatly increase the amount of optoelectronic data, and more accurately collect the signals of the entire process of the reaction cups and / or reactants. Subsequently, through calculation and analysis, the features for identifying abnormalities can be extracted to achieve better monitoring of the reaction process and provide guarantee for the accuracy and precision of the test results. Since the amount of optoelectronic data acquisition and analysis in this embodiment is much larger than the existing segmented acquisition method, the hardware is required to have stronger digital processing capabilities. Those skilled in the art can understand that the signal conditioning function in the circuit block diagram can be implemented not through device components on the hardware board, but also digitally in the processing module.

[0061] Since all signals of each wavelength are continuously collected, specifically as Figure 10 shown. Therefore, the edge of the code teeth signal of the optoelectronic code disk does not need to be precisely aligned with a specific position (such as the center) of each reaction cup, and the difficulty of part processing and assembly debugging can be greatly reduced. The edge of the code teeth signal of the optoelectronic code disk only needs to align the same reaction cup for each wavelength channel, and combined with an algorithm to identify signal characteristics such as peak value, valley value, pulse start point, pulse end point, pulse width, half-peak width, specific width, area, slope, rising edge time, falling time, etc. in each wavelength signal, and obtain the signal value of the calculation result. In some embodiments, the device that generates the edge of the code teeth signal of the optoelectronic code disk, such as an optocoupler, can also be cancelled. Only through signal processing methods, for example, collecting the pulse waveforms of the whole process of the reaction cup passing through the light source, and through waveform alignment, the signal value of the calculation result can be obtained, thereby saving hardware costs and debugging time.

[0062] In one embodiment, the controller is further configured to:

[0063] Monitor the state of the liquid in the reaction cup or the reaction cup according to at least two third optical signals;

[0064] Or, obtain the detection result of the sample added to the reaction cup according to at least two third optical signals.

[0065] In this embodiment, when detecting a sample, reagents can be first added to the reaction cup, and then the sample can be added to the reaction cup. The sample and the reagents are mixed to form a reaction solution. When there is only reagent in the reaction cup, the state of the reagent in the reaction cup, such as the pH value of the reagent, can be obtained through the third optical signal or the absorbance or transmittance obtained by detecting the third optical signal, and whether the reagent is abnormal can be determined by the obtained reagent state, such as the opening time being too long, expiration, or deterioration, etc.; when no reagent is placed, the state of the reaction cup, such as whether there are scratches on the reaction cup, whether the cleanliness of the reaction cup meets the standard, or whether there is residual liquid in the reaction cup, can be determined through the third optical signal or the absorbance or transmittance obtained by the third optical signal; it can be understood that the liquid in the reaction cup can also be a reaction solution prepared from reagents and samples, and whether the reaction solution is abnormal can be determined through the third optical signal or the absorbance or transmittance obtained by the third optical signal.

[0066] Since the reaction cuvette moves relative to the light source assembly; the third optical signal is a signal sequence formed by the relationship between the light intensity and the acquisition time. The third optical signal is collected when the reaction cuvette passes by the light source during each rotation cycle of the reaction disk of the sample analyzer. Then, it is possible to determine whether an abnormality occurs based on the signal curve formed by the light intensity and the acquisition time in the third optical signal. For example, when an abnormal change occurs in the signal curve, it can be determined that there is an abnormality in the reaction cuvette or the liquid in the reaction cuvette; alternatively, the absorbance or transmittance can also be obtained from the third optical signal, and the absorbance or transmittance is compared with the absorbance or transmittance corresponding to the liquid in the reaction cuvette. Whether an abnormality occurs is determined based on the comparison result. The absorbance or transmittance corresponding to the liquid in the reaction cuvette is related to the type of the liquid in the reaction cuvette.

[0067] During the process of monitoring the reaction cuvette or the liquid in the reaction cuvette, it is possible to determine whether an abnormality occurs by comparing the third optical signals corresponding to different wavelengths within the same rotation cycle of the reaction disk, or by comparing the third optical signals of the same wavelength in adjacent rotation cycles.

[0068] Figure 11 The schematic flowchart corresponding to an embodiment of the control method of the sample analyzer is shown. The control method of the sample analyzer includes:

[0069] Step S10, controlling the light source assembly of the sample analyzer to irradiate the reaction cuvette with a first optical signal, where the first optical signal is an optical signal of a mixed wavelength;

[0070] Step S20, parallelly collecting at least two wavelengths of third optical signals formed by the first optical signal emerging from the reaction cuvette. The third optical signal is obtained by splitting the second optical signal after the first optical signal passes through the reaction cuvette;

[0071] Step S30, monitoring the state of the liquid or the reaction cuvette in the reaction cuvette according to at least two third optical signals; or obtaining the detection result of the sample added to the reaction cuvette according to at least two of the third optical signals.

[0072] Optionally, the reaction cuvette moves relative to the light source assembly; the third optical signal is a signal sequence formed by the relationship between the light intensity and the acquisition time. The third optical signal is collected when the reaction cuvette passes by the light source during each rotation cycle of the reaction disk of the sample analyzer, as Figure 7 and Figure 8As shown, optoelectronic data of the entire process of multiple wavelengths are collected in parallel. When the reaction cup passes through the light source assembly once, instead of segmenting the time when the reaction cup passes through the light source assembly this time according to the number of wavelengths, and collecting the signals of a specific wavelength for each period of time, the signals of multiple wavelengths are collected in parallel at the same time, and the detection positions of multiple wavelengths are the same. The advantage of this solution is that by simultaneously obtaining the signals measured at the same position at multiple wavelengths in the same period of time, subsequent corrections can be made or the detection results can be obtained based on the signals measured at the same position.

[0073] In this embodiment, when detecting a sample, reagents can be first added to the reaction cup, and then the sample can be added to the reaction cup. The sample and the reagents are mixed to form a reaction solution. When there is only reagent in the reaction cup, the state of the reagent in the reaction cup, such as the pH value of the reagent, can be obtained through the third optical signal or the absorbance or transmittance obtained by detecting the third optical signal. Whether there is an abnormality in the reagent can be determined based on the obtained reagent state, such as the lid being opened for too long, etc.; when no reagent is placed, the state of the reaction cup can be determined through the third optical signal, the absorbance or transmittance obtained by the third optical signal, such as whether there are scratches on the reaction cup, whether the cleanliness of the reaction cup meets the standard, or whether there is residual liquid in the reaction cup; it can be understood that the liquid in the reaction cup can also be a reaction solution prepared from reagents and samples, and whether the reaction solution is abnormal can be determined through the third optical signal or the absorbance or transmittance obtained by the third optical signal.

[0074] The reaction cup moves relative to the light source assembly; the third optical signal is a signal sequence formed by the relationship between the light intensity and the acquisition time. When the reaction cup passes through the light source during each rotation period of the reaction disk of the sample analyzer, the third optical signal is collected. Then, whether there is an abnormality can be determined through the signal curve formed by the light intensity and the acquisition time in the third optical signal. For example, when there is an abnormal change in the signal curve, it can be determined that there is an abnormality in the reaction cup or the liquid in the reaction cup; alternatively, the absorbance or transmittance can also be obtained from the third optical signal, and the absorbance or transmittance is compared with the absorbance or transmittance corresponding to the liquid in the reaction cup, and whether there is an abnormality is determined based on the comparison result. The absorbance or transmittance corresponding to the liquid in the reaction cup is related to the type of the liquid in the reaction cup. Optionally, the state of the reaction cup or the liquid in the reaction cup can be determined by comparing the third optical signals of different wavelengths within the same rotation period of the reaction cup; alternatively, whether there is an abnormality can also be determined by comparing the third optical signals of the same wavelength in adjacent rotation periods of the reaction cup.

[0075] Optionally, in one embodiment, a certain wavelength is more sensitive to the detection of certain types of liquids. For example, certain wavelengths have a more obvious detection effect on lipid particles. Then, a third optical signal of a certain wavelength can be selected for status monitoring. Optionally, in one embodiment, the step of monitoring the status of the liquid or the reaction cup in the reaction cup according to at least two third optical signals in step S30 includes:

[0076] Obtain the third optical signal corresponding to the target wavelength among the third optical signals of at least two wavelengths within the same rotation period of the reaction disk;

[0077] Determine the status of the liquid or the reaction cup in the reaction cup according to the third optical signal corresponding to the target wavelength.

[0078] Optionally, the step of obtaining the third optical signal corresponding to the target wavelength among the third optical signals of at least two wavelengths within the same rotation period of the reaction disk includes:

[0079] Obtain the target detection item corresponding to the liquid in the reaction cup irradiated by the first optical signal;

[0080] Determine the third optical signal corresponding to the target wavelength among the third optical signals of at least two wavelengths within the same rotation period of the reaction disk according to the target detection item.

[0081] By selecting different target wavelengths for different detection items, the monitoring results of the reaction cup or the liquid in the reaction cup are made more accurate.

[0082] Optionally, in one embodiment, the step of obtaining the detection result of the sample added to the reaction cup according to at least two of the second optical signals includes:

[0083] Obtain the optical measurement result of the liquid in the reaction cup by obtaining at least two of the third optical signals detected in each rotation period;

[0084] Generate an optical measurement result curve of the sample according to the optical measurement results of each rotation period;

[0085] Obtain the detection result of the sample according to the optical measurement result curve.

[0086] As Figure 2 shown, the optical measurement result curve can be a corresponding relationship curve between absorbance and light collection points. In each period, the absorbance corresponding to each period can be obtained by processing the third optical signal. Each period corresponds to a light collection point. That is, obtaining the optical measurement result of the liquid in the reaction cup by obtaining at least two of the third optical signals detected in each rotation period includes:

[0087] Obtain the target detection item corresponding to the liquid in the reaction cup irradiated by the first optical signal;

[0088] Obtain the target wavelength corresponding to the target detection item in each of the rotation periods;

[0089] Obtain the optical measurement result of the rotation period according to the third optical signal corresponding to the target wavelength.

[0090] In another embodiment, the step of obtaining the optical measurement result of the liquid in the reaction cup by obtaining at least two of the third optical signals detected in each period includes:

[0091] Obtain the optical measurement results corresponding to each of the third optical signals within the same rotation period;

[0092] Obtain the optical measurement result within the rotation period according to the optical measurement results corresponding to each of the third optical signals within the same rotation period.

[0093] The optical measurement results of each of the third optical signals can be used to obtain the optical measurement result within the rotation period by means of linear fitting, averaging, or weighted averaging, which can be set as needed and will not be elaborated here.

[0094] In one embodiment, before the step of monitoring the state of the liquid or the reaction cup in the reaction cup according to at least two third optical signals; or, before the step of obtaining the detection result of the sample added to the reaction cup according to at least two of the third optical signals, further includes:

[0095] Obtain the first target signal and the abnormal second target signal in the third optical signals of the same rotation period;

[0096] Calibrate the second target signal with the first target signal.

[0097] It can be understood that for certain types of samples or certain types of detection items, the detection results of the optical signals of certain wavelengths are more accurate, and then the more accurate first target signal can be used to calibrate the abnormal second target signal, and the abnormal second target signal can be a signal with an abnormal peak or an abnormal trough.

[0098] Figure 5 Show a schematic flow corresponding to an embodiment of the control method of the sample analyzer, and the control method of the sample analyzer includes:

[0099] Control the light source assembly of the sample analyzer to irradiate the reaction cup with a first optical signal, and the first optical signal is an optical signal with a mixed wavelength;

[0100] Collect in parallel at least two wavelengths of third optical signals, and the third optical signals are obtained by splitting the second optical signal after the first optical signal exits from the reaction cup;

[0101] The third optical signal of the second wavelength is corrected by using the third optical signal of the first wavelength among at least two third optical signals.

[0102] Optionally, before the step of correcting the third optical signal of the second wavelength by using the third optical signal of the first wavelength among at least two third optical signals, it further includes:

[0103] Obtaining the detection item corresponding to the liquid in the reaction cup irradiated by the first optical signal;

[0104] Obtaining the third optical signal of the first wavelength corresponding to the detection item.

[0105] Optionally, the second optical signal is a signal sequence formed by the correspondence between the optical intensity and time, and the reaction cup moves relative to the light source assembly so that the detection positions corresponding to different times in the signal sequence are different.

[0106] Optionally, before the step of correcting the third optical signal of the second wavelength by using the third optical signal of the first wavelength among at least two third optical signals, it further includes:

[0107] Obtaining the abnormal third optical signal of the second wavelength within the same rotation period.

[0108] It can be understood that the abnormal third optical signal of the second wavelength can be a signal with an abnormal peak or an abnormal trough, and the third optical signal of the first wavelength can be a signal that is relatively accurate for the current detection item, and the first wavelength can be preset.

[0109] Those of ordinary skill in the art can understand that all or some of the steps in the methods disclosed above, and the functional modules / units in systems and devices, can be implemented as software, firmware, hardware, and their appropriate combinations. In a hardware embodiment, the division between the functional modules / units mentioned above does not necessarily correspond to the division of physical components; for example, one physical component can have multiple functions, or one function or step can be executed by several physical components in cooperation. Some or all physical components can be implemented as software executed by a processor, such as a central processing unit, a digital signal processor, or a microprocessor, or can be implemented as hardware, or can be implemented as an integrated circuit, such as an application-specific integrated circuit. Such software can be distributed on a computer-readable medium, which can include a computer storage medium (or non-transitory medium) and a communication medium (or transitory medium). As is well known to those of ordinary skill in the art, the term computer storage medium includes volatile and non-volatile, removable and non-removable media implemented in any method or technology for storing information, such as computer-readable instructions, data structures, program modules, or other data. Computer storage media includes, but is not limited to, RAM, ROM, EEPROM, flash memory, or other memory technologies, CD-ROM, digital versatile disk (DVD), or other optical disk storage, magnetic cassette, tape, magnetic disk storage, or other magnetic storage devices, or any other medium that can be used to store the desired information and can be accessed by a computer. In addition, as is well known to those of ordinary skill in the art, a communication medium typically contains computer-readable instructions, data structures, program modules, or other data in a modulated data signal such as a carrier wave or other transmission mechanism, and can include any information delivery medium.

[0110] It should be understood that the term "and / or" used in the specification and appended claims of this application refers to any combination and all possible combinations of one or more of the associated listed items, and includes these combinations. It should be noted that in this document, the term "comprises", "comprising", or any other variation thereof is intended to cover a non-exclusive inclusion, such that a process, method, article, or system that comprises a series of elements includes not only those elements but also other elements not expressly listed, or elements that are inherent to such process, method, article, or system. Without further limitation, an element defined by the phrase "comprising an..." does not exclude the existence of additional identical elements in the process, method, article, or system that comprises the element.

[0111] The serial numbers of the embodiments of the present application above are only for description and do not represent the superiority or inferiority of the embodiments. The above is only a specific embodiment of the present application, but the protection scope of the present application is not limited thereto. Any person skilled in the art within the technical scope disclosed by the present application can easily think of various equivalent modifications or substitutions, and these modifications or substitutions should all be covered within the protection scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.

Claims

1. A control method for a sample analyzer, characterized in that, Comprising: Controlling a light source assembly of a sample analyzer to irradiate a reaction cup with a first optical signal, the first optical signal being an optical signal with a mixed wavelength; Parallelly collecting at least two wavelengths of a third optical signal formed by the first optical signal emerging from the reaction cup, the third optical signal being obtained by splitting a second optical signal after the first optical signal emerges from the reaction cup; Monitoring the state of the liquid in the reaction cup or the reaction cup according to at least two third optical signals; Alternatively, obtaining a detection result of a sample added to the reaction cup according to at least two of the third optical signals.

2. The control method of the sample analyzer according to claim 1, characterized in that, The reaction cup moves relative to the light source assembly; the third optical signal is a signal sequence formed by the relationship between the optical intensity and the acquisition time, and the third optical signal is collected when the reaction cup passes through the light source within each rotation period of a reaction disk of the sample analyzer.

3. The control method of the sample analyzer according to claim 1, wherein The step of monitoring the state of the liquid in the reaction cup or the reaction cup according to at least two third optical signals includes: Obtaining a third optical signal corresponding to a target wavelength among at least two wavelengths of the third optical signal within the same rotation period of the reaction disk; Determining the state of the liquid in the reaction cup or the reaction cup according to the third optical signal corresponding to the target wavelength.

4. The control method of the sample analyzer according to claim 3, wherein, The step of obtaining a third optical signal corresponding to a target wavelength among at least two wavelengths of the third optical signal within the same rotation period of the reaction disk includes: Obtaining a target detection item corresponding to the liquid in the reaction cup irradiated by the first optical signal; Determining a third optical signal corresponding to the target wavelength among at least two wavelengths of the third optical signal within the same rotation period of the reaction disk according to the target detection item.

5. The control method of the sample analyzer according to claim 1, characterized in that, The step of monitoring the state of the liquid in the reaction cup or the reaction cup according to at least two third optical signals includes: Comparing at least two of the third optical signals within the same rotation period of the reaction cup; Determining the state of the liquid in the reaction cup or the reaction cup according to the comparison result.

6. The control method of the sample analyzer according to claim 1, wherein, The step of obtaining a detection result of a sample added to the reaction cup according to at least two of the third optical signals includes: Obtaining an optical measurement result of the liquid in the reaction cup by obtaining at least two of the third optical signals detected in each rotation period; Generating an optical measurement result curve of the sample according to the optical measurement results of each rotation period; Obtaining a detection result of the sample according to the optical measurement result curve.

7. The control method of the sample analyzer according to claim 6, characterized in that, The step of obtaining an optical measurement result of the liquid in the reaction cup by obtaining at least two of the third optical signals detected in each rotation period includes: Obtaining a target detection item corresponding to the liquid in the reaction cup irradiated by the first optical signal; Obtaining a target wavelength corresponding to the target detection item in each rotation period; Obtaining an optical measurement result of the rotation period according to the third optical signal corresponding to the target wavelength.

8. The control method of the sample analyzer according to claim 7, characterized in that, The step of obtaining an optical measurement result of the liquid in the reaction cup by obtaining at least two of the third optical signals detected in each period includes: Obtaining an optical measurement result corresponding to each of the third optical signals within the same rotation period; Obtaining an optical measurement result of the rotation period according to the optical measurement results corresponding to each of the third optical signals within the same rotation period.

9. The control method of the sample analyzer according to claim 1, characterized in that, Performing status monitoring on the liquid in the reaction cup or the reaction cup based on at least two second optical signals; Alternatively, before the step of obtaining the detection result of the sample added to the reaction cup according to at least two of the second optical signals, it further includes: Obtaining a first target signal and an abnormal second target signal in the third optical signal of the same rotation period; Calibrating the second target signal using the first target signal.

10. A control method for a sample analyzer, characterized in that, Including: Controlling the light source assembly of the sample analyzer to irradiate the reaction cup with a first optical signal, the first optical signal being an optical signal with a mixed wavelength; Parallelly collecting third optical signals of at least two wavelengths, the third optical signals being obtained by splitting the second optical signal after the first optical signal exits the reaction cup; Correcting the third optical signal of the second wavelength using the third optical signal of the first wavelength among at least two third optical signals.

11. The control method of the sample analyzer according to claim 10, characterized in that, Before the step of correcting the third optical signal of the second wavelength using the third optical signal of the first wavelength among at least two third optical signals, it further includes: Obtaining the detection item corresponding to the liquid in the reaction cup irradiated by the first optical signal; Obtaining the third optical signal of the first wavelength corresponding to the detection item.

12. The control method of the sample analyzer according to claim 10, characterized in that, The second optical signal is a signal sequence formed by the correspondence between the light intensity and time, and the reaction cup moves relative to the light source assembly so that the detection positions corresponding to different times in the signal sequence are different.

13. The control method of the sample analyzer according to claim 10, characterized in that, Before the step of correcting the third optical signal of the second wavelength using the third optical signal of the first wavelength among at least two third optical signals, it further includes: Obtaining the abnormal third optical signal of the second wavelength within the same rotation period.

14. A sample analyzer, characterized in that, Including a sample dispensing device, a reagent dispensing device, a reaction device, a detection device, and a controller; The sample dispensing device is used to aspirate a sample from the sample container scheduled to the sampling position and transport the aspirated sample to the reaction cup; The reagent dispensing device is used to dispense the reagent into the reaction cup; The reaction device is used to incubate the reaction solution in the reaction cup, the reaction solution being prepared from the reagent and the sample; The detection device, the detection device includes a light source assembly and an optical signal acquisition assembly, the light source assembly is used to emit a first optical signal to the reaction cup, the optical signal acquisition device includes a splitting device and at least two optical signal acquisition channels, the splitting device splits the second optical signal after the first optical signal exits the reaction cup into third optical signals of at least two wavelengths, and at least two of the optical signal acquisition assemblies parallelly collect the third optical signals, and the wavelengths of the third optical signals collected by different optical signal acquisition assemblies are different; The controller is used to process the third optical signal to obtain a processing result.

15. The sample analyzer according to claim 14, wherein, The optical signal acquisition assembly includes at least a photoelectric sensor and a signal processor, and the signal processor is used to process the electrical signal output by the photoelectric sensor to according to the processed electrical signal.

16. The sample analyzer according to claim 14, characterized in that, The reaction device includes a reaction disk, a driving device, and at least two reaction positions for placing the reaction cups. The driving device drives the reaction disk to rotate so that the reaction cups move relative to the light source assembly. It further includes a reaction driving assembly, which is used to drive the relative movement between the reaction cups and the light source assembly. The third optical signal is a signal sequence formed by the relationship between the light intensity and the acquisition time. The third optical signal is collected when the reaction cups pass by the light source during each rotation cycle of the reaction disk of the sample analyzer.

17. The sample analyzer according to claim 14, characterized in that, The controller is further configured to: Monitor the state of the liquid in the reaction cup or the reaction cup based on at least two third optical signals; Alternatively, obtain the detection result of the sample added to the reaction cup based on at least two of the third optical signals.

18. The sample analyzer according to claim 14, characterized in that, The controller is further configured to: Correct the third optical signal of the second wavelength by using the third optical signal of the first wavelength among at least two third optical signals.

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