Blood protein peptide hair-growing spray and preparation method thereof
By preparing sprays containing blood protein peptides and plant extracts, the problems of safety and low transdermal absorption of existing hair health products are solved, and the comprehensive improvement of efficient hair growth and hair follicle nutrition is achieved.
Patent Information
- Application Number
- CN202510575542.X
- Authority / Receiving Office
- CN · China
- Patent Type
- Applications(China)
- Current Assignee / Owner
- Filing Date
- 2025-05-06
- Publication Date
- 2025-08-01
AI Technical Summary
The existing hair health products have low safety ingredients, low transdermal absorption, dosage form defects and single functions, making it difficult to quickly improve hair loss problems.
The blood protein peptide and a variety of natural plant extracts are prepared in the form of a spray using transdermal penetration agent and solubilizer, which improves the transdermal absorption rate, improves the hair follicle environment and provides nutrition.
It achieves efficient transdermal absorption, high safety and easy to use hair growth effect, significantly improves hair follicle microcirculation and hair toughness, and comprehensively solves hair health problems.
Smart Images
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Figure BDA0005388437890000061
Abstract
Description
Technical Field
[0001] The present application relates to the field of hair care, and specifically to a blood protein peptide hair growth spray and a preparation method thereof. Background Art
[0002] Currently, in the field of hair care, traditional plant extract hair growth tinctures, such as Polygonum multiflorum and Platycladus orientalis extracts, although relatively safe, have large molecular weight active ingredients and low transdermal absorption rates, which makes the preparation take effect slowly and difficult to meet the needs of patients to quickly improve hair loss problems. In terms of dosage form, liquid tinctures or ointments have obvious defects. Their texture is sticky and easy to stick to the hair after spraying, affecting beauty and bringing inconvenience to users. In addition, the existing products have relatively single functions, mostly only focusing on anti-hair loss, lacking a comprehensive solution for improving follicle microcirculation, nutritional supply, and hair toughness improvement, and unable to fundamentally solve hair health problems.
[0003] With the progress of bioengineering technology, the development of new low-cost, high-performance, safe and reliable hair health products has become an urgent need. As a potential hair growth promoter, blood protein peptide has the advantages of wide source and easy large-scale production, which provides a new idea for overcoming traditional technical bottlenecks and can solve problems such as the uncertain safety and high price of existing hair growth promoting products during long-term use.
[0004] Therefore, it is of great significance to develop a hair growth spray with high transdermal absorption rate, safe and natural ingredients, and comprehensive hair growth promoting functions. Summary of the Invention
[0005] The object of the present invention is to provide a blood protein peptide hair growth spray and a preparation method thereof to solve problems such as low ingredient safety, low transdermal absorption rate of plant extracts, dosage form defects, and single function existing in existing hair growth products, and to achieve the effects of improving the follicle environment, providing follicle nutrition, and promoting hair growth.
[0006] In the first aspect, the present application provides a blood protein peptide hair growth spray, adopting the following technical solution: A blood protein peptide spray is composed of raw materials in the following mass percentages:
[0007] Optionally, the blood protein peptide is selected from one or more of MLL peptide segment and WDL peptide segment; the amino acid sequence of the MLL peptide segment is methionine-leucine-leucine; the amino acid sequence of the WDL peptide segment is tryptophan-aspartic acid-leucine.
[0008] Optionally, the preparation method of the plant extract includes: Step 1: Wash, dry Polygonum multiflorum Thunb., Codonopsis pilosula (Franch.) Nannf., Sophora flavescens Aiton, Astragalus membranaceus (Fisch.) Bunge, Zanthoxylum bungeanum Maxim., Artemisia argyi Levl. et Vant., Liquidambar formosana Hance, Tribulus terrestris L., Angelica sinensis (Oliv.) Diels, Dictamnus dasycarpus Turcz. respectively, and then pulverize them with an air flow pulverizer to obtain fine powder. Step 2: Add the obtained fine powder to a 70 - 95% ethanol solution, soak it, and then reflux and extract, and collect the extract. Step 3: Obtain the plant extract after rotary evaporation.
[0009] Optionally, the blood protein peptide is composed of MLL peptide segment and WDL peptide segment.
[0010] Optionally, the transdermal penetration enhancer is one or more of azone, oleic acid, propylene glycol, and terpene substances.
[0011] Optionally, the solubilizer is one or more of sodium dodecyl sulfate, Tween 80, and polyethylene glycol 400.
[0012] Optionally, the solvent is physiological saline.
[0013] In a second aspect, the present application provides a preparation method of a blood protein peptide hair growth spray, including the following steps: S1: Dissolve the blood protein peptide and liver protein hydrolysate in a solvent, and stir evenly to make a peptide mixture. S2: Continuously add the transdermal penetration enhancer, solubilizer, and plant extract under stirring, and mix evenly to obtain a stock solution. S3: Filter the stock solution, fill it into a spray bottle, and perform sealed packaging to finally obtain the blood protein peptide hair growth spray.
[0014] In a third aspect, the present application provides an application of the blood protein peptide hair growth spray provided in the first aspect in the preparation of a health product for promoting hair growth.
[0015] In summary, the present application includes at least one of the following beneficial technical effects: 1. Safe and natural ingredients: The blood protein peptide hair growth spray of the present application adopts a variety of natural plant ingredients, blood protein peptide, and liver protein hydrolysate, avoiding the use of chemical drugs, reducing side effects such as scalp allergies, swelling, and hormone dependence, with high safety and being suitable for long-term use. 2. Good transdermal absorption: By using the more easily absorbed blood protein peptide and a reasonable formula design, the molecular weight of the blood protein peptide as the active ingredient is small, improving the transdermal absorption rate, being able to play a role faster, improving the hair follicle environment, and promoting hair growth. 3. Obvious dosage form advantages: The spray dosage form is convenient to use, has a refreshing texture, does not stick to the hair, and does not affect beauty.
[0016] 4. Comprehensive and integrated functions: The product not only has an anti - hair - loss effect, but also can improve the microcirculation of hair follicles, provide rich nutrition for hair follicles, enhance the toughness of hair, solve hair health problems from multiple aspects, and the effect is remarkable. Detailed implementation manners
[0017] Definition In the present invention, unless otherwise specified, scientific and technical terms used herein have the meanings commonly understood by those skilled in the art. To better understand the present invention, the definitions and explanations of relevant terms are provided below.
[0018] In this article, the term "liver protein hydrolysate" refers to the product obtained by hydrolyzing liver proteins. It is usually obtained by crushing, enzymatic hydrolysis or acid hydrolysis of the livers of healthy animals, so that the large - molecular proteins in the liver are degraded into smaller peptide segments and amino acids. These hydrolysis products contain a variety of essential and non - essential amino acids, small peptides, and some other bioactive substances such as nucleotides and trace elements.
[0019] In this article, the term "blood protein peptide" refers to a new active peptide segment screened from blood proteins, abbreviated as new active peptide, which has at least one of the following active characteristics: (a) activating inflammatory cells; (b) promoting the migration, differentiation and / or proliferation of vascular endothelial cells; and (c) promoting hair growth.
[0020] In some embodiments, the blood protein peptide is selected from one or more of the MLL peptide segment and the WDL peptide segment; the amino acid sequence of the MLL peptide segment is methionine - leucine - leucine; the amino acid sequence of the WDL peptide segment is tryptophan - aspartic acid - leucine.
[0021] The present invention is further described in detail below with reference to embodiments. Unless otherwise specified, the raw materials are all from conventional commercial sources.
[0022] Preparation Example 1 Preparation of blood protein peptide Blood protein peptides were screened from bovine blood protein solution after enzymatic hydrolysis, namely the WDL (TrpAspLeu) peptide segment and the MLL (MetLeuLeu) peptide segment. The WDL peptide segment and the MLL peptide segment were respectively synthesized by solid - phase synthesis method using an automatic peptide synthesizer (synthesized by Nanjing Yuanpei Biotechnology Co., Ltd.), purified by high - performance liquid chromatography (HPLC), and the synthesized peptides were identified by methods such as mass spectrometry (MS) and amino acid sequence analysis to confirm their sequences and purities. Finally, blood protein peptides with a purity greater than 95% were obtained.
[0023] Preparation Example 2 Preparation of plant extract Step 1: Raw material pretreatment and micropowder preparation Wash 200 g of fleece-flower root, 50 g of codonopsis pilosula, 50 g of lightyellow sophora root, 50 g of astragalus membranaceus, 80 g of Chinese prickly ash, 50 g of mugwort leaf, 50 g of Chinese honey locust, 50 g of puncturevine caltrop fruit, 50 g of Chinese angelica, and 50 g of dictamnus dasycarpus respectively, dry them, and then pulverize them using an air-flow pulverizer (set the pulverizing pressure at 8 bar, the feeding pressure at 10 bar, and the feeding speed at 65 g / min) to obtain fine powder; Step 2: Extraction Add the obtained fine powder to 6 times the amount of 85% ethanol solution and mix. After soaking for 3 h, reflux and extract 3 times at 55°C, and collect the extracts separately; Step 3: Concentration Combine the above-obtained extracts, cool them to room temperature, and obtain a plant extract after rotary evaporation.
[0024] Preparation Example 3 Preparation of liver protein hydrolysate Take 50 kg of fresh bovine liver, crush and homogenize it using a colloid mill, and pour the homogenate into a stirring tank; add 3 times the weight of sterile water based on the weight of the added bovine liver, add 0.5% of papain based on the weight of the bovine liver, and enzymatically hydrolyze at 50°C for 4 hours. After the reaction, heat the material liquid to 80°C and inactivate the enzyme for 30 minutes; wait for the temperature of the material liquid to drop to 30°C, concentrate it to 30% of the original volume, and then ultrafilter it using a 30 KD hollow fiber membrane to retain macromolecular proteins and microorganisms, and finally obtain liver protein hydrolysate.
[0025] Example 1 A blood protein peptide hair growth spray Raw material composition of a blood protein peptide hair growth spray: 1% of the plant extract prepared in Preparation Example 2, 3% of blood protein peptide MLL peptide segment, 3% of blood protein peptide WDL peptide segment, 2% of the liver protein hydrolysate prepared in Preparation Example 3, 2% of transdermal penetration enhancer azone, 5% of solubilizer polyethylene glycol 400, and the balance is physiological saline.
[0026] Preparation method of the above spray: S1: Dissolve the blood protein peptide and liver protein hydrolysate in physiological saline and stir evenly to prepare a peptide mixture; S2: Continue to add azone, polyethylene glycol 400, and the plant extract prepared in Preparation Example 2 under stirring, and mix evenly to obtain a stock solution; S3: Fill the stock solution filtered through a 0.22 μm filter membrane into a spray bottle, and perform sealed packaging to finally obtain a blood protein peptide hair growth spray.
[0027] Example 2 A blood protein peptide hair growth spray Raw material composition of a blood protein peptide hair growth spray: 0.5% of the plant extract prepared in Preparation Example 2, 1% of blood protein peptide MLL peptide segment, 1% of blood protein peptide WDL peptide segment, 1% of the liver protein hydrolysate prepared in Preparation Example 3, 2% of transdermal penetration enhancer azone, 5% of solubilizer polyethylene glycol 400, and the balance is physiological saline.
[0028] The preparation method of the above spray is the same as that of Example 1.
[0029] Example 3 A Hemoprotein Peptide Hair Growth Spray The raw material composition of a hemoprotein peptide hair growth spray: 3% of hemoprotein peptide MLL peptide segment, 3% of hemoprotein peptide WDL peptide segment, 2% of the liver protein hydrolyzate prepared in Preparation Example 3, 2% of the transdermal penetration enhancer azone, 5% of the solubilizer polyethylene glycol 400, and the balance is physiological saline.
[0030] The preparation method of the above spray: S1: Dissolve the hemoprotein peptide and the liver protein hydrolyzate in physiological saline and stir evenly to prepare a peptide mixture; S2: Continuously add azone and polyethylene glycol 400 under stirring and mix evenly to obtain a stock solution; S3: Fill the stock solution filtered through a 0.22 μm filter membrane into a spray bottle, carry out sealed packaging, and finally obtain the hemoprotein peptide hair growth spray.
[0031] Example 4 A Hemoprotein Peptide Hair Growth Spray The raw material composition of a hemoprotein peptide hair growth spray: 1% of the plant extract prepared in Preparation Example 2, 3% of hemoprotein peptide MLL peptide segment, 3% of hemoprotein peptide WDL peptide segment, 2% of the transdermal penetration enhancer azone, 5% of the solubilizer polyethylene glycol 400, and the balance is physiological saline.
[0032] The preparation method of the above spray: S1: Dissolve the hemoprotein peptide in physiological saline and stir evenly; S2: Continuously add azone, polyethylene glycol 400 and the plant extract prepared in Preparation Example 2 under stirring and mix evenly to obtain a stock solution; S3: Fill the stock solution filtered through a 0.22 μm filter membrane into a spray bottle, carry out sealed packaging, and finally obtain the hemoprotein peptide hair growth spray.
[0033] Comparative Example 1 The raw material composition of a spray: 1% of the plant extract prepared in Preparation Example 2, 2% of the transdermal penetration enhancer azone, 5% of the solubilizer polyethylene glycol 400, and the balance is physiological saline.
[0034] The preparation method of the above spray: S1: Stir and mix azone, polyethylene glycol 400 and the plant extract prepared in Preparation Example 2 evenly to obtain a stock solution; S2: Fill the stock solution filtered through a 0.22 μm filter membrane into a spray bottle, carry out sealed packaging, and finally obtain the spray.
[0035] Comparative Example 2 Raw material composition of a spray: 1% of the plant extract of Preparation Example 2, 2% of the liver protein hydrolyzate of Preparation Example 3, 2% of the transdermal penetration enhancer azone, 5% of the solubilizer polyethylene glycol 400, and the balance is physiological saline.
[0036] Preparation method of the above spray: S1: Dissolve the liver protein hydrolyzate in physiological saline and stir evenly; S2: Continuously add azone, polyethylene glycol 400 and the plant extract of Preparation Example 2 under stirring, and mix evenly to obtain the stock solution; S3: Fill the stock solution filtered through a 0.22 μm filter membrane into a spray bottle, and perform sealed packaging to finally obtain the spray.
[0037] In-vivo efficacy detection of the blood protein peptide spray of Example 5 5.1 Safety test Select 50 healthy experimental mice and randomly divide them into 5 groups with 10 mice in each group. Spray the blood protein peptide sprays prepared in Examples 1-4 and the commercially available minoxidil tincture hair growth spray on the back skin of the mice respectively, once a day for 14 consecutive days.
[0038] Observe the changes in the skin of the mice. No adverse reactions such as skin allergy and redness were found in the 4 groups of mice in Examples 1-4, which proves that the blood protein peptide spray of the present invention has good safety, while 30% of the mice showed skin allergy and redness with the commercially available minoxidil tincture hair growth spray.
[0039] 5.2 Efficacy test Select 120 volunteers with hair loss problems and randomly divide them into 6 groups with 20 volunteers in each group. Use the blood protein peptide sprays prepared in Examples 1, 2, 3, 4 and the sprays of Comparative Example 1 and Comparative Example 2 respectively, twice a day for 3 months. Observe the hair loss situation, hair density and hair quality changes of the volunteers. The results are shown in Table 1.
[0040] Table 1 According to the results in Table 1: Examples 1-4 all contain blood protein peptides (MLL / WDL), and their hair loss reduction rates (52.4%-68.5%) and hair density increases (18.7%-29.3%) are significantly higher than those of Comparative Example 1 (22.3%, 6.2%) and Comparative Example 2 (35.6%, 10.5%) without blood protein peptides, indicating that blood protein peptides play a key promoting role in follicle activity and hair growth. The reason is that, on the one hand, the transdermal absorption rate of the active ingredients of the spray containing blood protein peptides is higher than that of the spray containing only plant extracts; on the other hand, the blood protein peptides (MLL / WDL) of the present application activate inflammatory cells such as mast cells, promote the expression of angiogenesis factors, enable the migration, differentiation, and / or proliferation of vascular endothelial cells, improve the local blood supply around the hair follicles, and significantly promote hair growth.
[0041] Example 1 (containing 10% plant extract) is significantly superior to Example 3 (without plant extract, 58.9%, 24.6%) in terms of hair loss reduction rate (68.5%) and hair density (29.3%), indicating that plant components such as Polygonum multiflorum Thunb. and Astragalus membranaceus Bunge may enhance the hair follicle microenvironment through antioxidant and nutritional supply.
[0042] Comparing Example 1 (containing liver protein hydrolysate) with Example 4 (without liver protein hydrolysate), the former has a higher increase in hair density (29.3% vs. 26.8%), suggesting that liver protein hydrolysate may further optimize the effect by regulating local metabolism.
[0043] The effect of Example 2 (low-concentration blood protein peptides + 5% plant extract) is weaker than that of Example 1 (high-concentration blood protein peptides + 10% plant extract), indicating that the dose of the active ingredient is positively correlated with the effect, and the formula concentration needs to be balanced to achieve the best cost performance.
[0044] Blood protein peptides (MLL / WDL) are the core efficacy components of this spray. They play a role by improving the local blood supply around the hair follicles; plant extracts provide antioxidant support, and liver protein hydrolysates assist in metabolic regulation. The three work together to form a multi-target hair growth mechanism. Due to the synergistic effect of high-concentration active ingredients and a complete formula, Example 1 exhibits the optimal comprehensive effect, and its safety is significantly superior to commercially available minoxidil products (no allergic reactions), showing potential for clinical promotion.
[0045] Those skilled in the art will further recognize that the present invention can be implemented in other specific forms without departing from its spirit or central characteristics. Since the foregoing description of the present disclosure only discloses its exemplary embodiments, it should be understood that other variations are considered to be within the scope of the present invention. Therefore, the present invention is not limited to the specific embodiments described in detail herein. Instead, the scope and content of the present invention should be indicated by reference to the appended claims.
Claims
1. A blood protein peptide hair growth spray, characterized in that, It is composed of raw materials with the following mass percentages: Plant extract: 0.5% - 1%; Blood protein peptide: 2% - 6%; Liver protein hydrolysate: 1% - 2%; Transdermal penetration enhancer: 1% - 5%; Solubilizer: 2% - 8%; Solvent: make up to 100%.
2. The hair growth spray containing blood protein peptides according to claim 1, characterized in that, The blood protein peptide is selected from one or more of MLL peptide segment and WDL peptide segment; the amino acid sequence of the MLL peptide segment is methionine - leucine - leucine; the amino acid sequence of the WDL peptide segment is tryptophan - aspartic acid - leucine.
3. The hair growth spray containing blood protein peptides according to claim 1, characterized in that, The preparation method of the plant extract includes: Step 1: Wash, dry Polygonum multiflorum, Codonopsis pilosula, Sophora flavescens, Astragalus membranaceus, Zanthoxylum bungeanum, Artemisia argyi, Liquidambar formosana, Tribulus terrestris, Angelica sinensis, Dictamnus dasycarpus respectively, and then use an air flow pulverizer to pulverize them to obtain fine powder; Step 2: Add the obtained fine powder to a 70 - 95% ethanol solution, soak and then reflux extract, and collect the extract; Step 3: Obtain the plant extract after rotary evaporation.
4. The hair growth spray containing blood protein peptides according to claim 1, characterized in that, The blood protein peptide is composed of MLL peptide segment and WDL peptide segment.
5. The hair growth spray containing blood protein peptides according to claim 1, wherein, The transdermal penetration enhancer is one or more of azone, oleic acid, propylene glycol, terpene substances.
6. The hair growth spray containing blood protein peptides according to claim 1, wherein The solubilizer is one or more of sodium dodecyl sulfate, Tween 80 and polyethylene glycol 400.
7. The hair growth spray containing blood protein peptides according to claim 1, characterized in that, The solvent is physiological saline.
8. A preparation method of the blood protein peptide hair growth spray according to any one of claims 1-7, characterized in that, It includes the following steps: S1: Dissolve the blood protein peptide and liver protein hydrolysate in the solvent, and stir evenly to make a peptide mixture; S2: Continuously add the transdermal penetration enhancer, solubilizer and plant extract under stirring, and mix evenly to obtain a stock solution; S3: Filter the stock solution, fill it into a spray bottle, and carry out sealed packaging to finally obtain the blood protein peptide hair growth spray.
9. Use of the blood protein peptide hair growth spray according to any one of claims 1 - 7 in the preparation of a health product for promoting hair growth.