High-efficiency propagation method for single-bud seedling raising of original seedling main stem of healthy sugarcane seedling

Abstract

The invention discloses an efficient propagation method for single-bud seedling culture of original seedlings of healthy sugarcane seedlings, and relates to the technical field of propagation of sugarcane seedlings. According to the propagation expanding, after 6-7 nodes are cultivated on sugarcane detoxification tissue culture seedlings, each node is cut off to obtain 6-7 single-bud stem nodes, the single-bud stem nodes are pretreated with a coating agent, seedling emergence cultivation is conducted on the pretreated single-bud stem nodes, and seedlings capable of being transplanted in a field can be obtained when 3-4 leaves grow on each seedling. By optimizing the propagation method, original seedlings can be propagated to 6-7 times, the propagation period is short, the obtained plantlets are high in germination rate, high in tillering, high in disease resistance and high in yield after being transplanted to a field, efficient propagation of the original seedlings is achieved, the purposes of reducing cost and increasing efficiency are achieved, and the method is suitable for application and popularization.

Description

Technical Field

[0001] The invention relates to the technical field of sugarcane seedling propagation, and in particular to a method for efficiently propagating healthy sugarcane seedlings by raising a single bud from the main stem of the original seedling. Background Art

[0002] Healthy sugarcane seedlings are virus-free tissue-cultured sugarcane seedlings, derived from disease-free sugarcane plants cultured from young heart leaves or stem apex meristems. These seedlings boast rapid growth, strong tillering ability, high stem formation rate, high yield, and minimal seed requirements. They accelerate the rapid propagation of improved sugarcane varieties, eliminating the degradation of superior varieties and the resulting decline in quality and yield caused by farmers' private seed reserves. This addresses the issue of large sugarcane seed consumption due to high seed requirements. However, sugarcane seedlings are currently expensive, costing 1-1.5 yuan per plant. This high planting cost necessitates efficient propagation to reduce costs and increase efficiency. Regarding the propagation of healthy sugarcane seedlings, the applicant has conducted relevant research (Chen Rongfa, Qiu Lihang, Zhou Huiwen, et al. Rapid propagation technology of the main stem of the original sugarcane virus-free healthy seedlings [J]. Guangxi Botany, 2021, 41(4): 614-621.), that is, after the healthy sugarcane seedlings have tillered, the main stem of the original seedling is cut off and repeated cuttings and transplanting are performed twice to obtain healthy seedlings. This technology reduces the cost of seed propagation to a certain extent and speeds up the propagation speed, but the applicant believes that the propagation speed needs to be improved. Based on this, the applicant tried to use the single bud segment of the main stem of the original seedling for propagation to achieve rapid and efficient propagation. However, due to the small biomass of the single bud segment of the main stem of the original seedling, there are problems such as low survival rate, weak seedlings obtained by propagation, and poor transplanting effect. After many experiments and improvements, the applicant obtained this solution, obtained strong seedlings with a high survival rate, and achieved efficient propagation of the original seedlings. Summary of the Invention

[0003] In view of the above shortcomings, the present invention provides a method for efficiently propagating healthy sugarcane seedlings from a single bud on the main stem, which solves the problems of low efficiency in propagating healthy sugarcane seedlings, low survival rate, and weak seedlings. The specific technical solution is as follows:

[0004] A method for efficiently propagating single-bud seedlings from the main stem of healthy sugarcane seedlings is disclosed. The propagation comprises: culturing 6-7 nodes of virus-free sugarcane tissue culture seedlings, cutting each node to obtain 6-7 single-bud stem nodes, pre-treating the single-bud stem nodes with a coating agent, and culturing the pre-treated single-bud stem nodes for seedling emergence. When the seedlings grow 3-4 leaves, seedlings suitable for field transplanting are obtained.

[0005] Preferably, the virus-free sugarcane tissue culture seedlings are virus-free tissue culture seedlings of sugarcane varieties with strong tillering ability, high yield and high sugar content.

[0006] Preferably, the coating agent comprises the following raw materials in parts by weight: 0.4-0.9 parts of cytokinin, 0.1-0.5 parts of quercetin, 0.2-0.6 parts of indolebutyric acid, 0.5-1.2 parts of prochloraz, 1.0-1.5 parts of carbendazim, 0.5-0.8 parts of baicalein, 10-15 parts of glucose, 3-8 parts of potassium dihydrogen phosphate, 1-7 parts of ammonium chloride, 2-8 parts of mica powder, 25-33 parts of carboxymethyl cellulose and 800-1000 parts of water.

[0007] Preferably, the coating agent is prepared by slowly adding carboxymethyl cellulose to water and stirring until completely dissolved, then adding mica powder and stirring thoroughly at 30-40°C to obtain a mixed solution A, and then adding the remaining materials to the mixed solution A in sequence and mixing and stirring evenly to obtain the coating agent.

[0008] Preferably, the pretreatment is: placing the single bud stem segment into the coating agent for a first soaking for 5-10 minutes, taking it out, drying it, then soaking it for a second time for 20-30 minutes, drying it, and germinating it at 30-35°C for 2-3 days.

[0009] Preferably, the seedling emergence cultivation is to place the pretreated single bud stem node into a seedling tray containing a matrix for cultivation, specifically: first place a layer of the matrix in the seedling tray, then place the pretreated single bud stem node, and finally cover it with a layer of the matrix with a thickness of 0.2-0.5 cm, water it thoroughly, and apply plant extract when the young shoots break through the soil; the plant spacing is 5-10 cm, the row spacing is 10-15 cm, the matrix is kept moist during the cultivation process, and the cultivation temperature is 25-30°C.

[0010] Preferably, the matrix comprises the following raw materials in parts by weight: 40-50 parts of modified coconut husk, 20-30 parts of livestock and poultry manure, 18-25 parts of crop straw, 15-20 parts of peat, 6-15 parts of perlite, 10-15 parts of loess, 1-3 parts of Pseudomonas putida, 0.5-1.5 parts of phosphate-solubilizing bacteria, 2-5 parts of arbuscular mycorrhizal fungi and 0.1-0.5 parts of Trichoderma harzianum.

[0011] Preferably, the modified coconut chaff is prepared by crushing the coconut chaff and then soaking it in water for 1-2 hours, then adding 30-60 IU / g of hemicellulase, enzymolyzing it at room temperature for 8-12 hours, and drying it to a moisture content of 60-70% to obtain the modified coconut chaff.

[0012] Preferably, the preparation method of the substrate is as follows: taking the raw materials in parts by weight, the modified coconut husk, livestock and poultry manure, crop straw, peat, perlite, loess, Pseudomonas putida, phosphate-solubilizing bacteria, arbuscular mycorrhizal fungi and Trichoderma harzianum are mixed, adjusting the moisture content of the mixture to 60-75%, and fermenting at 25-35° C. for 20-30 days to obtain the substrate.

[0013] Preferably, the preparation method of the plant extract is: 10-30 parts of Murraya osmanthus, 1-8 parts of cloves, 2-5 parts of orris roots and 15-20 parts of garlic are mixed and crushed, the mixture is added into water which is 50-60 times by weight of the mixture, and soaked for 10-15 hours, filtered to obtain an extract, and rhamnolipid in an amount of 1-3% by weight of the extract is added to the extract, and the mixture is mixed and stirred evenly to obtain the plant extract.

[0014] Compared with the prior art, the present invention has the following beneficial effects:

[0015] 1. The present invention can multiply one original seedling to obtain 6-7 robust seedlings by optimizing the propagation method, and the propagation cycle is short. The obtained seedlings have a high germination rate when transplanted into the field, many tillers, strong disease resistance, and high yield, thus realizing efficient propagation of original seedlings and achieving the purpose of reducing costs and increasing efficiency, and is suitable for promotion and application.

[0016] 2. The present invention uses sugarcane virus-free tissue culture seedlings containing 6-7 nodes for propagation. Compared with the propagation of mature sugarcane virus-free tissue culture seedlings, this saves the propagation cycle. However, due to the small biomass of the single bud segments of sugarcane virus-free tissue culture seedlings containing 6-7 nodes, there are problems such as low survival rate, weak seedlings obtained through propagation, and poor transplanting and planting effects. The present application solves the above problems by using an optimized coating agent and culture matrix, and optimizing the management method, thereby improving the survival rate and obtaining strong seedlings.

[0017] 3. The cytokinin, quercetin and ammonium chloride in the coating agent of the present invention act together to promote tillering of single bud seed stems, indolebutyric acid can promote rooting of single bud seed stems, glucose, potassium dihydrogen phosphate and ammonium chloride act together to provide sufficient nutrients for single bud seed stems, prochloraz, carbendazim and baicalein act together to inhibit the occurrence of pests and diseases, carboxymethyl cellulose can be used as a film-forming agent to coat other raw materials on the seed stem, and mica powder can improve the stability of the film-forming agent, making it difficult to fall off and causing other agents to lose effectiveness. Therefore, in the coating agent, each raw material interacts with each other, improves the seed stem germination rate, survival rate and tillering rate, and achieves strong roots, strong buds and strong seedlings.

[0018] 4. The matrix of the present invention is obtained by composting and fermenting modified coconut husk, livestock and poultry manure, crop straw, peat, perlite, loess and microbial agents. It has excellent water retention, air permeability and heat insulation properties, and can provide sufficient nutrients for seedlings. Among them, Pseudomonas putida, phosphate-solubilizing bacteria, arbuscular mycorrhizal fungi and Trichoderma harzianum can secrete a large amount of extracellular polysaccharides during the mixed fermentation process. These polysaccharides can serve as adhesives to bond the nutrients in livestock and poultry manure with modified coconut husk, peat and perlite to form a stable aggregate structure, thereby improving the nutrient adsorption capacity of modified coconut husk, peat and perlite, preventing the nutrient from osmotic loss during watering, and enabling the seedlings to obtain sufficient nutrients. At the same time, after mixed fermentation, the modified coconut husk has stronger heat preservation and water retention properties, and the air permeability of peat and perlite is very beneficial to the growth and development of the seedlings. In addition, after mixed fermentation, the microbial agents can also secrete hormones to stimulate the root growth of the seedlings and promote tillering. Therefore, the matrix of the present invention can make the seedlings grow quickly, has the effects of strengthening roots, buds and seedlings, and shortens the cultivation time.

[0019] 5. The present invention applies plant extracts when the young shoots break through the soil. The plant extracts and the agents in the coating agent complement each other to resist the invasion of pests and diseases on the young shoots, reduce the use of chemical agents, and improve the survival rate of the seedlings. BRIEF DESCRIPTION OF THE DRAWINGS

[0020] In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the following briefly introduces the drawings required for describing the embodiments.

[0021] Figure 1 These are photos of the original seedlings and the newly cut single bud seed stems;

[0022] Figure 2 The diagrams for seed stem treatment are shown, from left to right, in order: Example 1, Comparative Example 2, Comparative Example 3, Comparative Example 4, Comparative Example 5, and Comparative Example 1;

[0023] Figure 3 This is a picture of Example 1 when the stem has grown 2-3 leaves;

[0024] Figure 4 This is a picture of the comparative example 1 when the stem has grown 2-3 leaves;

[0025] Figure 5 This is a comparison of the growth of the seedlings of Example 1 and Comparative Example 6 two months after transplanting. DETAILED DESCRIPTION

[0026] The specific embodiments of the present invention are described in detail below, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments.

[0027] Example 1

[0028] A method for efficiently propagating single buds from the main stem of healthy sugarcane seedlings comprises: selecting virus-free tissue culture seedlings of a sugarcane variety with strong tillering ability, high yield, and high sugar content, cultivating six nodes, cutting each node to obtain six single-bud stem nodes (1-1.5 cm long), placing the single-bud stem nodes in a coating agent, soaking them once for 5 minutes, removing them, drying them, then soaking them a second time for 20 minutes, drying them, and germinating them at 30°C for 3 days to obtain pretreated single-bud stem nodes. , place the pretreated single bud stem nodes in a seedling tray containing a substrate for cultivation. Specifically: first place a layer of substrate in the seedling tray, then place the pretreated single bud stem nodes, and finally cover with a layer of substrate with a thickness of 0.2 cm, water it thoroughly, and apply plant extract when the young shoots break through the soil; the plant spacing is 5 cm and the row spacing is 10 cm. Keep the substrate moist during the cultivation process, the cultivation temperature is 25°C, and when the seedlings grow 3-4 leaves, you can obtain seedlings that can be used for field transplanting.

[0029] The coating agent comprises the following raw materials in parts by weight: 0.4 parts of cytokinin, 0.1 parts of quercetin, 0.2 parts of indolebutyric acid, 0.5 parts of prochloraz, 1.0 parts of carbendazim, 0.5 parts of baicalein, 10 parts of glucose, 3 parts of potassium dihydrogen phosphate, 1 part of ammonium chloride, 2 parts of mica powder, 25 parts of carboxymethyl cellulose, and 800 parts of water. The preparation method of the coating agent is as follows: first, carboxymethyl cellulose is slowly added to water and stirred until completely dissolved, then mica powder is added and stirred thoroughly at 30° C. to obtain a mixed solution A, and then the remaining materials are added to the mixed solution A in sequence and mixed and stirred evenly to obtain the coating agent.

[0030] The matrix comprises the following raw materials by weight: 40 parts modified coconut husk, 20 parts livestock and poultry manure, 18 parts crop straw, 15 parts peat, 6 parts perlite, 10 parts loess, 1 part Pseudomonas putida, 0.5 parts phosphate-solubilizing bacteria, 2 parts arbuscular mycorrhizal fungi, and 0.1 parts Trichoderma harzianum. The modified coconut husk is prepared by first crushing the coconut husk and soaking it in water for 1 hour, then adding 30 IU / g of hemicellulase, hydrolyzing it at room temperature for 12 hours, and then drying it to a moisture content of 60%. The matrix is prepared by taking the raw materials by weight, mixing the modified coconut husk, livestock and poultry manure, crop straw, peat, perlite, Pseudomonas putida, phosphate-solubilizing bacteria, arbuscular mycorrhizal fungi, and Trichoderma harzianum, adjusting the moisture content of the mixture to 60%, and fermenting it at 25°C for 30 days.

[0031] The preparation method of the plant extract comprises the following steps: mixing and crushing 10 parts of Murraya osmanthus, 1 part of cloves, 2 parts of orris root and 15 parts of garlic, adding the mixture into water with a volume of 50 times the weight of the mixture, soaking for 15 hours, filtering to obtain an extract, adding rhamnolipid in an amount of 1% by weight of the extract into the extract, and mixing and stirring uniformly to obtain the plant extract.

[0032] Example 2

[0033] A method for efficiently propagating single buds from the main stem of healthy sugarcane seedlings comprises: selecting virus-free tissue culture seedlings of a sugarcane variety with strong tillering ability, high yield, and high sugar content, cultivating seven nodes, cutting each node to obtain seven single-bud stem nodes (1-1.5 cm long), placing the single-bud stem nodes in a coating agent, soaking them for 10 minutes, removing them, drying them, then soaking them for a second time for 30 minutes, drying them, and germinating them at 35°C for 2 days to obtain pretreated single-bud stem nodes. , place the pretreated single bud stem nodes in a seedling tray containing a substrate for cultivation. Specifically: first place a layer of substrate in the seedling tray, then place the pretreated single bud stem nodes, and finally cover with a layer of substrate with a thickness of 0.3 cm. Water it thoroughly, and apply plant extract when the young shoots break through the soil; the plant spacing is 10 cm and the row spacing is 15 cm. Keep the substrate moist during the cultivation process, the cultivation temperature is 30°C, and when the seedlings grow 3-4 leaves, you can obtain seedlings that can be used for field transplanting.

[0034] The coating agent comprises the following raw materials in parts by weight: 0.9 parts of cytokinin, 0.5 parts of quercetin, 0.6 parts of indolebutyric acid, 1.2 parts of prochloraz, 1.5 parts of carbendazim, 0.8 parts of baicalein, 15 parts of glucose, 8 parts of potassium dihydrogen phosphate, 7 parts of ammonium chloride, 8 parts of mica powder, 33 parts of carboxymethyl cellulose, and 1000 parts of water. The preparation method of the coating agent is as follows: first, carboxymethyl cellulose is slowly added to water and stirred until completely dissolved, then mica powder is added and stirred thoroughly at 40° C. to obtain a mixed solution A, and then the remaining materials are added to the mixed solution A in sequence and mixed and stirred evenly to obtain the coating agent.

[0035] The matrix comprises the following raw materials by weight: 50 parts modified coconut husk, 30 parts livestock and poultry manure, 25 parts crop straw, 20 parts peat, 15 parts perlite, 15 parts loess, 3 parts Pseudomonas putida, 1.5 parts phosphate-solubilizing bacteria, 5 parts arbuscular mycorrhizal fungi, and 0.5 parts Trichoderma harzianum. The modified coconut husk is prepared by first crushing the coconut husk and soaking it in water for 2 hours. Then, 60 IU / g of hemicellulase is added, and after enzymatic hydrolysis at room temperature for 8 hours, the enzyme is added and dried to a moisture content of 70%. The matrix is prepared by mixing the raw materials by weight, including the modified coconut husk, livestock and poultry manure, crop straw, peat, perlite, Pseudomonas putida, phosphate-solubilizing bacteria, arbuscular mycorrhizal fungi, and Trichoderma harzianum, adjusting the moisture content of the mixture to 75%, and fermenting it at 35°C for 20 days.

[0036] The preparation method of the plant extract comprises the following steps: mixing and crushing 30 parts of Murraya osmanthus, 8 parts of cloves, 5 parts of orris roots and 20 parts of garlic, adding the mixture into water with a volume of 60 times the weight of the mixture, soaking for 10 hours, filtering to obtain an extract, adding rhamnolipid in an amount of 3% by weight of the extract into the extract, and mixing and stirring uniformly to obtain the plant extract.

[0037] Example 3

[0038] A method for efficiently propagating single buds from the main stem of healthy sugarcane seedlings comprises: selecting virus-free tissue culture seedlings of a sugarcane variety with strong tillering ability, high yield, and high sugar content, cultivating seven nodes, cutting each node to obtain seven single-bud stem nodes (1-1.5 cm long), placing the single-bud stem nodes in a coating agent, soaking them once for 8 minutes, removing them, drying them, then soaking them a second time for 25 minutes, drying them, and germinating them at 32°C for 3 days to obtain pretreated single-bud stem nodes. , place the pretreated single bud stem nodes in a seedling tray containing a substrate for cultivation. Specifically: first place a layer of substrate in the seedling tray, then place the pretreated single bud stem nodes, and finally cover with a layer of substrate with a thickness of 0.5 cm, water it thoroughly, and apply plant extract when the young shoots break through the soil; the plant spacing is 8 cm and the row spacing is 12 cm. Keep the substrate moist during the cultivation process, the cultivation temperature is 28°C, and when the seedlings grow 3-4 leaves, you can obtain seedlings that can be used for field transplanting.

[0039] The coating agent comprises the following raw materials in parts by weight: 0.6 parts of cytokinin, 0.3 parts of quercetin, 0.5 parts of indolebutyric acid, 0.9 parts of prochloraz, 1.3 parts of carbendazim, 0.6 parts of baicalein, 13 parts of glucose, 5 parts of potassium dihydrogen phosphate, 2 parts of ammonium chloride, 6 parts of mica powder, 30 parts of carboxymethyl cellulose, and 900 parts of water. The preparation method of the coating agent is as follows: first, carboxymethyl cellulose is slowly added to water and stirred until completely dissolved, then mica powder is added and stirred thoroughly at 35° C. to obtain a mixed solution A, and then the remaining materials are added to the mixed solution A in sequence and mixed and stirred evenly to obtain the coating agent.

[0040] The matrix comprises the following raw materials by weight: 45 parts modified coconut husk, 25 parts livestock and poultry manure, 22 parts crop straw, 17 parts peat, 12 parts perlite, 12 parts loess, 2 parts Pseudomonas putida, 0.8 parts phosphate-solubilizing bacteria, 3 parts arbuscular mycorrhizal fungi, and 0.2 parts Trichoderma harzianum. The modified coconut husk is prepared by first crushing the coconut husk and soaking it in water for 2 hours. Then, 50 IU / g of hemicellulase is added, and after enzymatic hydrolysis at room temperature for 10 hours, the husk is dried to a moisture content of 65%. The matrix is prepared by mixing the raw materials by weight, including the modified coconut husk, livestock and poultry manure, crop straw, peat, perlite, Pseudomonas putida, phosphate-solubilizing bacteria, arbuscular mycorrhizal fungi, and Trichoderma harzianum, adjusting the moisture content of the mixture to 70%, and fermenting it at 30°C for 25 days.

[0041] The preparation method of the plant extract comprises the following steps: mixing and crushing 20 parts of Murraya osmanthus, 4 parts of cloves, 3 parts of orris root and 17 parts of garlic, adding the mixture into water with a volume of 55 times the weight of the mixture, soaking for 12 hours, filtering to obtain an extract, adding rhamnolipid in an amount of 2% by weight of the extract into the extract, and mixing and stirring uniformly to obtain the plant extract.

[0042] Comparative Example 1: The seed stems were treated with water instead of the coating agent, and the other steps were the same as those in Example 1.

[0043] Comparative Example 2: The coating agent does not contain quercetin and ammonium chloride, and the other steps are the same as those in Example 1.

[0044] Comparative Example 3: The coating agent does not contain glucose and potassium dihydrogen phosphate, and the other steps are the same as those in Example 1.

[0045] Comparative Example 4: The coating agent does not contain carbendazim and baicalein, and the other steps are the same as those in Example 1.

[0046] Comparative Example 5: The coating agent does not contain mica powder, and the other steps are the same as those in Example 1.

[0047] Guitang No. 44 was used as a propagation variety, and the propagation results of Examples 1 to 3 and Comparative Examples 1 to 5 were statistically analyzed, as shown in Table 1. Here, survival rate = (number of survivors / number of seedings) × 100%; the growth period is the time from the time a pretreated single bud node is placed in a seedling tray containing a substrate for cultivation until 3-4 leaves grow on the small surface.

[0048] Table 1 Results of each group

[0049]

[0050]

[0051] Table 1 shows that the propagation method of the present invention can achieve a survival rate of over 95%, a short growth period of less than 20 days, and strong seedlings. Furthermore, each raw material in the coating agent of the present invention has a significant impact on the survival rate, growth period, and growth of the seedlings. Figure 3 (Picture of Example 1 when the stem has grown 2-3 leaves) and Figure 4 (Comparative Example 1: Picture of a stem with 2-3 leaves growing out) By comparison, it can be seen that the seedlings obtained by the propagation method of the present invention are more robust.

[0052] The seedlings obtained by propagation in each group of Examples 1 to 3 and Comparative Examples 1 to 5 were transplanted, and 100 plants were selected for transplanting in each group. The management methods of each group were consistent. The tillering rate and the incidence rate were counted 2 months after transplanting, and the final yield was counted. The results are shown in Table 2, where the incidence rate is the incidence of smut observed by randomly selecting 50 plants in each group, and the incidence rate (%) = (number of diseased seedlings / total number of surveys) × 100%.

[0053] Table 2 Conditions of each group after transplanting

[0054] Group Tillering rate (%) Incidence (%) Yield (t / mu) Example 1 290 2 7.9 Example 2 298 0 8.1 Example 3 298 0 8.2 Comparative Example 1 261 16 7.0 Comparative Example 2 278 12 7.5 Comparative Example 3 281 12 7.7 Comparative Example 4 274 10 7.4 Comparative Example 5 275 10 7.5

[0055] Table 2 shows that the tillering rate and yield of the seedlings obtained by the propagation of the present invention after transplantation are both high, and the disease incidence is low. In addition, each raw material in the coating agent of the present invention has a significant impact on the transplanting tillering rate, disease incidence and yield.

[0056] Comparative Example 6: A conventional substrate (components: coconut husk, peat, perlite, and decomposed compost) was used instead of the substrate in Example 1 for cultivation. Other steps were the same as in Example 1.

[0057] Comparative Example 7: The substrate was directly mixed with modified coconut husk, livestock and poultry manure, crop straw, peat, perlite and microbial agent without fermentation treatment, that is, not fermented at 25° C. for 30 days before use. Other steps were the same as in Example 1.

[0058] Comparative Example 8: When the young shoots break through the soil, water is used instead of the plant extract for watering. Other steps are the same as those in Example 1.

[0059] Guitang No. 44 was used as a propagation variety, and the propagation results of Example 1 and Comparative Examples 6 to 8 were statistically analyzed, as shown in Table 3. The statistical methods of various indicators were the same as above.

[0060] Table 3 Results of each group

[0061]

[0062]

[0063] Table 3 shows that the matrix of the present invention can increase the survival rate and shorten the cultivation period, and has the effects of strengthening roots, buds and seedlings. At the same time, the plant extract of the present invention can also improve the survival rate.

[0064] The seedlings obtained by propagation in each group of Example 1 and Comparative Examples 6 to 8 were transplanted using the same transplanting method as above. The results are shown in Table 4:

[0065] Table 4 Conditions of each group after transplanting

[0066] Group Tillering rate (%) Incidence (%) Yield (t / mu) Example 1 290 2 7.9 Comparative Example 6 277 10 7.5 Comparative Example 7 279 6 7.6 Comparative Example 8 288 6 7.8

[0067] Table 4 shows that the matrix composition and preparation method have a significant impact on transplant tillering rate, disease incidence and yield. The matrix of the present invention can significantly increase tillering rate and yield and reduce disease incidence. Figure 5 It shows that the seedlings of Example 1 grew well and tillered quickly 2 months after transplanting (the treatment group in the figure), while the seedlings of Comparative Example 6 had fewer tillers 2 months after transplanting (the control group in the figure).

[0068] In summary, the present invention can propagate 6-7 strong seedlings from one original seedling by optimizing the propagation method, and the propagation cycle is short. The obtained seedlings have a high germination rate, many tillers, strong disease resistance, and high yield when transplanted into the field, thereby realizing efficient propagation of original seedlings and achieving the purpose of reducing costs and increasing efficiency, and is suitable for promotion and application.

[0069] The foregoing descriptions of specific exemplary embodiments of the present invention are for purposes of illustration and description. These descriptions are not intended to limit the invention to the precise forms disclosed, and it is apparent that many variations and modifications are possible in light of the foregoing teachings. The exemplary embodiments have been selected and described for the purpose of explaining the specific principles of the invention and their practical application, thereby enabling those skilled in the art to realize and utilize a variety of exemplary embodiments of the invention and various options and modifications. The scope of the invention is intended to be defined by the claims and their equivalents.

Claims

1. A method for efficiently propagating healthy sugarcane seedlings from a single bud on the main stem, characterized in that: The propagation method comprises the following steps: after the sugarcane virus-free tissue culture seedlings have 6-7 nodes, each node is cut to obtain 6-7 single-bud stem nodes, the single-bud stem nodes are pretreated with a coating agent, and the pretreated single-bud stem nodes are cultured for seedling emergence, and seedlings that can be used for field transplanting are obtained when the seedlings grow 3-4 leaves.

2. The method for efficiently propagating healthy sugarcane seedlings from a single bud on the main stem according to claim 1, characterized in that: The virus-free sugarcane tissue culture seedlings are selected from sugarcane varieties with strong tillering ability, high yield and high sugar content.

3. The method for efficiently propagating healthy sugarcane seedlings from a single bud on the main stem according to claim 1, characterized in that: The coating agent comprises the following raw materials in parts by weight: 0.4-0.9 parts of cytokinin, 0.1-0.5 parts of quercetin, 0.2-0.6 parts of indolebutyric acid, 0.5-1.2 parts of prochloraz, 1.0-1.5 parts of carbendazim, 0.5-0.8 parts of baicalein, 10-15 parts of glucose, 3-8 parts of potassium dihydrogen phosphate, 1-7 parts of ammonium chloride, 2-8 parts of mica powder, 25-33 parts of carboxymethyl cellulose and 800-1000 parts of water.

4. The method for efficiently propagating healthy sugarcane seedlings from a single bud on the main stem according to claim 3, characterized in that: The coating agent is prepared by slowly adding carboxymethyl cellulose into water and stirring until completely dissolved, then adding mica powder and stirring thoroughly at 30-40° C. to obtain a mixed solution A, and then adding the remaining materials to the mixed solution A in sequence and stirring uniformly to obtain the coating agent.

5. The method for efficiently propagating healthy sugarcane seedlings from a single bud on the main stem according to claim 1, characterized in that: The pretreatment comprises: placing the single bud stem segment into the coating agent for a first soaking for 5-10 minutes, taking it out, drying it, then soaking it for a second time for 20-30 minutes, drying it, and accelerating germination at 30-35° C. for 2-3 days.

6. The method for efficiently propagating healthy sugarcane seedlings from a single bud on the main stem according to claim 1, characterized in that: The seedling emergence cultivation is to place the pretreated single bud stem node into a seedling tray containing a matrix for cultivation, specifically: firstly place a layer of the matrix into the seedling tray, then place the pretreated single bud stem node, and finally cover with a layer of the matrix with a thickness of 0.2-0.5 cm, water it thoroughly, and apply plant extract when the young shoots break through the soil; the plant spacing is 5-10 cm and the row spacing is 10-15 cm, the matrix is kept moist during the cultivation process, and the cultivation temperature is 25-30°C.

7. The method for efficiently propagating healthy sugarcane seedlings from a single bud on the main stem according to claim 6, characterized in that: The matrix comprises the following raw materials in parts by weight: 40-50 parts of modified coconut husk, 20-30 parts of livestock and poultry manure, 18-25 parts of crop straw, 15-20 parts of peat, 6-15 parts of perlite, 10-15 parts of loess, 1-3 parts of Pseudomonas putida, 0.5-1.5 parts of phosphate-solubilizing bacteria, 2-5 parts of arbuscular mycorrhizal fungi and 0.1-0.5 parts of Trichoderma harzianum.

8. The method for efficiently propagating healthy sugarcane seedlings from a single bud on the main stem according to claim 7, characterized in that: The preparation method of the modified coconut chaff comprises the following steps: firstly crushing the coconut chaff, soaking it in water for swelling for 1-2 hours, then adding 30-60 IU / g of hemicellulase, performing enzymolysis at room temperature for 8-12 hours, and drying it to a moisture content of 60-70% to obtain the modified coconut chaff.

9. The method for efficiently propagating healthy sugarcane seedlings from a single bud on the main stem according to claim 7, characterized in that: The preparation method of the matrix comprises the following steps: taking raw materials in parts by weight, mixing the modified coconut husk, livestock and poultry manure, crop straw, peat, perlite, loess, Pseudomonas putida, phosphate-solubilizing bacteria, arbuscular mycorrhizal fungi and Trichoderma harzianum, adjusting the water content of the mixture to 60-75%, and fermenting the mixture at 25-35° C. for 20-30 days to obtain the matrix.

10. The method for efficiently propagating healthy sugarcane seedlings from a single bud on the main stem according to claim 6, characterized in that: The preparation method of the plant extract comprises the following steps: mixing and crushing 10-30 parts of Murraya osmanthus, 1-8 parts of cloves, 2-5 parts of orris roots, and 15-20 parts of garlic; adding the mixture into water that is 50-60 times the weight of the mixture, soaking for 10-15 hours, filtering to obtain an extract; and adding rhamnolipid in an amount of 1-3% by weight of the extract into the extract, mixing and stirring uniformly to obtain the plant extract.

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