Acupuncture microneedle combined with exosome injection

Abstract

The invention belongs to the technical field of medical apparatus and instruments, and particularly relates to an acupuncture microneedle combined with exosome injection, which comprises an acupuncture needle body and an exosome injection device, and the exosome injection device is movably mounted on the acupuncture needle body; the acupuncture needle body comprises a needle body and a needle handle integrally connected with the needle body, the needle body and the needle handle are respectively provided with an axially communicated cavity, an airtight diaphragm is arranged in the cavity at the joint of the needle body and the needle handle, and the exosome injection device is inserted into the cavity of the needle handle. The independent disposable exosome injection device is prepared and combined with the improved acupuncture needle, after air entraining operation of meridians and collaterals in the acupuncture process is completed, exosomes are injected into acupuncture points, and a good clinical effect is achieved.

Description

Technical Field

[0001] The present invention belongs to the technical field of medical devices, and specifically relates to a microneedle for acupuncture combined with exosome injection. Background Art

[0002] Acupuncture, as one of the traditional Chinese medicine therapies, regulates the balance of Qi and blood, and Yin and Yang by stimulating the acupuncture points corresponding to specific meridians on the body, thereby achieving the purpose of treating and preventing diseases. Acupuncture mainly targets acupuncture points on the limbs. The Jing, Rong, Shu, Jing, He, and Yuan points of each meridian are located between the knee joint and the toes and between the elbow joint and the fingertips. During the treatment process, no medication is involved, avoiding the side effects of drugs and damage to organs. At the same time, compared with surgical treatment, the cost of treatment is low. Therefore, acupuncture treatment is safe and does not cause serious side effects. Acupuncture treatment has now received attention in most countries.

[0003] Exosomes are ubiquitous nano-membrane vesicles containing a variety of proteins, messenger RNAs (mRNAs), and microRNAs. They play a crucial role in intercellular material transport and information transmission through direct interaction with signaling molecules on the cell membrane surface, intracellular regulation of membrane fusion contents, and the release of bioactive components. Their role in the cellular microenvironment is increasingly recognized, and they hold broad development prospects. They are considered an essential component of many biological functions and may serve as an alternative to cell therapy.

[0004] CN119174857 A discloses a microneedle for exosome injection. The disclosed technical solution is a microneedle for exosome injection, comprising a syringe, a plunger, and a needle body. The syringe contains exosomes secreted or engineered from neural stem cells or immune cells. One end of the plunger is slidably connected to the syringe, while the other end extends outside the syringe. The needle body is connected to the syringe, and the sharp end of the needle body is inserted into the acupuncture point. The plunger moves toward the needle body to inject the exosomes into the acupuncture point from within or outside the needle body. The method of use is as follows: When a Chinese medicine practitioner or acupuncturist needs to perform acupuncture on a patient, they first insert the hollow needle body into the acupuncture point. After the needle is inserted, the syringe containing the exosomes is screwed into the thread of the needle body. Then, the thumb and ring finger hold the needle body, the middle finger supports the syringe body, and the index finger pushes the plunger to push the exosomes in the syringe body through the needle body and into the acupuncture point. After the exosomes are pushed in, the syringe body is unscrewed, and the needle body remains at the acupuncture point until the treatment time is completed.

[0005] During use, this technical solution starts with the injection of exosomes after the needle is inserted into the acupuncture point, and then the needle is twisted. During the twisting of the needle, exosomes inevitably flow out from the outer edge of the needle. Moreover, acupuncturists or Chinese medicine practitioners are well aware that acupuncture includes two basic techniques: tonification and drainage. For the vast majority of patients, tonification is the most common method. Inserting a hollow needle into an acupuncture point causes the gas in the meridians to leak out, which is contrary to the tonification method and cannot achieve the technical effect envisioned in this technical solution. Summary of the Invention

[0006] The purpose of the present invention is to provide a microneedle for acupuncture combined with exosome injection to solve the problem of medical devices combining acupuncture and exosome injection therapy in the above-mentioned background technology.

[0007] To achieve the above objectives, this application is implemented through the following technical solutions:

[0008] An acupuncture microneedle combined with exosome injection comprises an acupuncture needle body and an exosome injection device, wherein the exosome injection device is movably mounted on the acupuncture needle body;

[0009] The acupuncture needle body includes a needle body and a needle handle integrally connected to the needle body. The needle body and the needle handle are both provided with an axially connected cavity, and an airtight diaphragm is provided in the cavity at the connection between the needle body and the needle handle. The exosome injection device is inserted into the cavity of the needle handle.

[0010] Furthermore, the diameter of the inner cavity of the needle body is smaller than the diameter of the inner cavity of the needle handle.

[0011] Furthermore, the diameter of the inner cavity of the needle handle is the same as the outer diameter of the exosome injection device.

[0012] Furthermore, multiple layers of wire wrapping are provided on the outer surface of the needle handle.

[0013] Furthermore, a detachable sealing plug or sealing membrane is provided at the rear end of the needle handle.

[0014] Furthermore, the exosome injection device includes a barrel, a needle is provided at the front end of the barrel, the needle is connected to the barrel, a push rod is provided in the barrel, the exosome accommodating cavity formed by the front end of the push rod and the barrel is filled with exosomes, the rear end of the push rod is connected to the compression spring, and the rear end of the compression spring is connected to the button.

[0015] Furthermore, the stroke of the button in the cylinder is smaller than the axial length of the exosome containing cavity.

[0016] Furthermore, a protective cover is provided at the front end of the cylinder body, and the protective cover is sleeved on the front end of the cylinder body. A needle accommodating cavity is provided in the protective cover, and the needle is accommodated in the needle accommodating cavity.

[0017] Furthermore, the exosomes are 3D exosomes, and the 3D exosomes are prepared by culturing 2D cells in a 3D culture system.

[0018] Furthermore, the 3D exosomes are stem cells, immune cells, and engineered exosomes.

[0019] The beneficial effects of the present invention are:

[0020] The present invention prepares an independent disposable exosome injection device, combines it with an improved acupuncture needle, and injects exosomes into acupuncture points after the qi-inducing operation of the meridians is completed during acupuncture, which has a good clinical effect. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 Schematic diagram of the microneedle structure for acupuncture combined with exosome injection of the present invention.

[0022] Figure 2 It is a structural schematic diagram of the acupuncture needle body of the present invention.

[0023] Figure 3 Schematic diagram of the structure of the exosome injection device of the present invention.

[0024] Description of reference numerals:

[0025] 1. Acupuncture needle body; 2. Exosome injection device; 11. Needle body; 12. Needle handle; 13. Airtight diaphragm; 14. Sealing plug or sealing membrane; 15. Wrapping wire; 21. Cylinder body; 22. Needle tip; 23. Push rod; 24. Compression spring; 25. Button; 26. Exosome holding chamber. DETAILED DESCRIPTION

[0026] The technical solution of the present invention is described in detail below with reference to the accompanying drawings. The following embodiments are merely exemplary and can only be used to explain and illustrate the technical solution of the present invention, and cannot be interpreted as limiting the technical solution of the present invention.

[0027] like Figures 1 to 3 As shown, the present application provides an acupuncture microneedle combined with exosome injection, which can achieve functions similar to acupuncture and at the same time can inject exosomes during the acupuncture process or at the corresponding acupuncture points. The microneedle of the present application includes an acupuncture needle body and an exosome injection device. The exosome injection device is movably mounted on the acupuncture needle body. Specifically, when no exosome injection is performed or when not in use, the acupuncture needle body and the exosome injection device are placed separately. Regarding the packaging method, the present application has no special requirements, as long as it can meet the preservation of exosomes, such as low-temperature storage or vacuum storage. During use, the exosome injection device 2 is connected to the acupuncture needle body 1 according to the timing of exosome injection.

[0028] The acupuncture needle body 1 of the present application includes a needle body 11 and a needle handle 12 integrally connected to the needle body. The appearance of the acupuncture needle body here is substantially consistent with that of currently used acupuncture needles. The specifications of the acupuncture needle body are directly referenced to those of currently used acupuncture needles. In the present application, both the needle body and the needle handle are provided with an axially connected cavity, that is, the acupuncture needle body of the present application has a cavity. Providing an axial cavity in an acupuncture needle is already possible in the prior art. For example, similar needles currently used for injecting insulin, growth hormone, etc. all have an axial cavity structure.

[0029] In the present application, the diameter of the inner cavity of the needle body is different from the diameter of the inner cavity of the needle handle, and the diameter of the inner cavity of the needle body is smaller than the diameter of the inner cavity of the needle handle. An airtight diaphragm is provided in the cavity at the connection between the needle body and the needle handle. The provision of the airtight diaphragm is for the use of acupuncture. It is well known in the field of acupuncture that during the acupuncture process, different acupoints are used for tonification and drainage according to the patient's condition, and during the tonification process, it is necessary to prevent the needle hole from leaking air. Therefore, the present technical solution provides an airtight diaphragm 13 in the cavity. On the other hand, if the airtight diaphragm 13 is not provided in the cavity of the acupuncture needle body, the internal air in the meridians will leak out during the acupuncture process, and the acupuncture effect will not be achieved.

[0030] On the other hand, in this technical solution, exosome injection is not performed in the early stage of acupuncture to avoid the exosomes overflowing from the needle hole during the acupuncture process, especially during the needle twisting or lifting process, thereby reducing the effect.

[0031] In the present application, a multi-layer wire wrapping 15 is provided on the outer surface of the needle handle 12. The specific wire wrapping method or style is the existing technology, and a direct reference can be made to the wire wrapping at the needle handle of the current acupuncture needle. The technical solution of the present application does not involve any improvement to the wire wrapping on the outer surface of the needle handle.

[0032] In the present application, a detachable sealing plug or sealing membrane 14 is provided at the rear end of the needle handle. The provision of the sealing plug or sealing membrane 14 is also to avoid the occurrence of acupuncture theory problems such as air deflation during the early acupuncture process. When the acupuncture needle needs to be connected to the exosome injection device, just pull out the sealing plug and tear off the sealing membrane.

[0033] In the present application, the exosome injection device 2 includes a barrel 21, and a needle 22 is provided at the front end of the barrel. In the present application, the needle 22 and the barrel 21 are fixedly connected rather than movably connected, that is, the needle cannot be separated from the barrel without destruction to avoid leakage of exosomes. A push rod 23 is provided in the barrel, and the front end of the push rod 23 and the inner cavity of the barrel form an exosome accommodating chamber 26. The exosomes are contained in the exosome accommodating chamber, and the rear end of the push rod is connected to the compression spring 24, and the rear end of the compression spring 24 is connected to the button 25.

[0034] In this application, the outer diameter of the barrel (i.e., the exosome injection device) is the same as the diameter of the needle shaft lumen, allowing the barrel to be inserted into the lumen of the needle shaft. In other embodiments of this application, including those in which the barrel cannot be inserted into the lumen of the needle shaft (due to its smaller diameter and relatively greater manufacturing difficulty), the needle of the exosome injection device is inserted into the lumen of the needle shaft and directly penetrates the airtight septum. The specific structure is designed based on the capabilities of the manufacturer.

[0035] The exosome injection device of the present application is used when the acupuncture needle is inserted and the corresponding needle twisting and other actions are completed, and in the middle and late stages of needle retention. Specifically, for example, if the needle needs to be retained for 20 minutes, the exosome injection is started after the needle is retained for 10 minutes, and the same applies to other times. If the needle does not need to be retained, the exosome injection can be performed after the needle twisting and other actions are completed.

[0036] In the present application, the stroke of the button in the cylinder is less than the axial length of the exosome containing cavity. That is to say, the technical solution of the present application is not to inject exosomes by continuously pressing the button, but to release the compression spring after pressing the button. Under the action of the restoring force, the compression spring compresses the push rod to move, thereby pressing the exosomes into the patient's acupuncture points through the needle tip and the acupuncture needle body. Moreover, this compression process is not under the action of human power, and the restoring force of the compression spring remains basically uniform or the rate of change of the restoring force is uniform, thereby preventing the exosomes from overflowing from the acupuncture port and allowing them to completely penetrate into the human body from the acupuncture point.

[0037] In the technical solution of the present application, a protective cover is provided at the front end of the barrel, and the protective cover is sleeved on the front end of the barrel. A needle accommodating cavity is provided inside the protective cover, and the needle is accommodated in the needle accommodating cavity. When not in use, the needle will not be exposed and can prevent exosomes from leaking from the needle, that is, the front end of the needle accommodating cavity can seal the front end of the needle.

[0038] In the present application, the exosomes are 3D exosomes, which are prepared from 2D cells in a 3D culture system. The 3D exosomes in the present application are stem cells, immune cells or engineered exosomes. For details of the 3D exosomes in the present application, please refer to the applicant's previous application documents. The preparation of 3D exosomes is not described here.

[0039] The working principle of this application is:

[0040] The acupuncture needles and exosome injection device are packaged separately. During use, the acupuncturist follows the principles of Traditional Chinese Medicine (TCM) and acupuncture points based on the patient's condition. The acupuncture techniques used vary depending on the acupuncturist's school of thought, including needle twisting and pulling movements for tonification and drainage. During the acupuncture process, some acupoints require a period of retention, while others do not. These procedures are performed during the exosome injection. Here we take the acupuncture points that need to be retained as an example to illustrate. Only when acupuncture and the retention of needles are started, the injection of exosomes is usually started halfway through the preparation for the retention of needles. The specific method is to tear off the outer packaging of the exosome injection device, and then tear off the sealing plug or sealing membrane at the rear end of the acupuncture needle body, and then pull off the protective cover to expose the needle tip, and then insert the needle tip from the rear end of the acupuncture needle body into the needle handle cavity, and continue to push forward until at least the needle tip passes through the airtight diaphragm, and then press the button. At this time, the compression spring is released. At this time, the compression spring is restored to its natural state from the original compressed state. At this time, the restoring force of the compression spring presses the push rod forward, and at the same time pushes the 3D exosomes into the human acupuncture point.

[0041] The above are only preferred embodiments of the present invention. It should be pointed out that for ordinary technicians in this technical field, several improvements and modifications can be made without departing from the principles of the present invention. These improvements and modifications should also be regarded as the scope of protection of the present invention.

Claims

1. A microneedle for acupuncture combined with exosome injection, characterized in that: It includes an acupuncture needle body and an exosome injection device, wherein the exosome injection device is movably mounted on the acupuncture needle body; The acupuncture needle body includes a needle body and a needle handle integrally connected to the needle body. The needle body and the needle handle are both provided with an axially connected cavity, and an airtight diaphragm is provided in the cavity at the connection between the needle body and the needle handle. The exosome injection device is inserted into the cavity of the needle handle.

2. The microneedle for acupuncture combined with exosome injection according to claim 1, characterized in that The diameter of the inner cavity of the needle body is smaller than the diameter of the inner cavity of the needle handle.

3. The microneedle for acupuncture combined with exosome injection according to claim 2, characterized in that: The diameter of the inner lumen of the needle handle is the same as the outer diameter of the exosome injection device.

4. The microneedle for acupuncture combined with exosome injection according to claim 1, characterized in that Multiple layers of wire wrapping are arranged on the outer surface of the needle handle.

5. The microneedle for acupuncture combined with exosome injection according to claim 1, characterized in that A detachable sealing plug or sealing diaphragm is provided at the rear end of the needle handle.

6. The microneedle for acupuncture combined with exosome injection according to claim 1, characterized in that The exosome injection device includes a barrel, a needle is provided at the front end of the barrel, the needle is connected to the barrel, a push rod is provided in the barrel, the exosome accommodating cavity formed by the front end of the push rod and the barrel is filled with exosomes, the rear end of the push rod is connected to the compression spring, and the rear end of the compression spring is connected to the button.

7. The microneedle for acupuncture combined with exosome injection according to claim 6, characterized in that: The stroke of the button in the cylinder is smaller than the axial length of the exosome containing cavity.

8. The microneedle for acupuncture combined with exosome injection according to claim 6, characterized in that: A protective cover is provided at the front end of the cylinder body, and the protective cover is sleeved on the front end of the cylinder body. A needle accommodating cavity is provided in the protective cover, and the needle is accommodated in the needle accommodating cavity.

9. The microneedle for acupuncture combined with exosome injection according to claim 6, characterized in that: The exosomes are 3D exosomes, which are prepared by using 2D cells in a 3D culture system.

10. The microneedle for acupuncture combined with exosome injection according to claim 9, characterized in that: The 3D exosomes are stem cells, immune cells, and engineered exosomes.

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