Strong reducing substance reducing agent for flooded rice field and application of strong reducing substance reducing agent

Abstract

The invention relates to a strong reducing substance reducing agent for a flooded rice field and application of the strong reducing substance reducing agent. According to the method, peracetic acid is adopted as a reducing agent, so that strong reducing substances accumulated in the flooded rice field are effectively reduced, and the influence of the strong reducing substances on soil and rice growth is reduced. Peracetic acid is applied to waterflooding potted plants added with commercial organic fertilizers and straw organic fertilizers and field tests, and results show that compared with blank control (without adding the peracetic acid) and negative control (with adding calcium peroxide), the method has the advantages that the oxidation-reduction potential of soil is remarkably improved and can be improved to 205-288mV, and the soil quality is improved. The total amount of reducing substances is reduced by 26.79% at most, the content of active reducing substances is reduced by 73.84% at most, and the growth and yield of rice are promoted. The invention provides a new technology for improving the oxidation-reduction potential and reducing the reducing substances in the flooded rice field after the organic fertilizer is applied so as to promote the yield increase of the rice.

Description

Technical Field

[0001] The invention relates to a flooded rice field reducing substance reducing agent generated by applying organic fertilizer and application thereof, belonging to the field of agricultural production. Background Art

[0002] Organic fertilizer plays a crucial role in agricultural production and is the primary source of fertilizer for green, organic foods. When applied to the soil, organic fertilizer improves soil structure, enhances water and fertilizer retention, promotes crop root growth and nutrient absorption, and, being rich in organic matter, effectively enhances soil microbial and enzyme activity, improving soil biofertility. Chemical fertilizers are highly nutritious and effective, but excessive and prolonged application can lead to soil compaction and acidification. Combining organic fertilizers with chemical fertilizers can improve fertilizer utilization, reduce the use and cost of chemical fertilizers and pesticides, and reduce environmental pollution and greenhouse gas emissions.

[0003] When rice fields are exposed to long-term flooding, returning straw to the fields or applying organic fertilizer produces large amounts of reducing substances. Excessive reducing substances can increase soil toxicity, affect soil microbial activity, and alter soil physical and chemical properties, such as reducing the redox potential. This, in turn, affects the soil's ecological environment, leading to root rot and impairing water and nutrient absorption, thus inhibiting normal rice plant growth.

[0004] To address the accumulation of strong reducing substances caused by excessive organic matter input into flooded rice paddies, treatment methods primarily include engineering drainage technology, balanced fertilization, and the addition of amendments. Water conservancy projects and tillage practices are currently common agricultural measures for reducing reducing substances in rice paddies. They can increase soil permeability, elevate redox potential, and reduce reducing substances, but this method requires a significant amount of human resources, is time-consuming and labor-intensive, and cannot be widely adopted. Applying soil amendments such as calcium peroxide or lime to rice paddies can improve soil redox status in a short period of time, but can also cause soil compaction after drainage. Both methods improve soil physical and chemical properties and significantly reduce strong reducing substances, but they are short-lived and can impact the soil microbial environment.

[0005] Peracetic acid is an organic peroxide, a colorless liquid with an acetic-like odor. Its molecular formula is CH3COOOH, and its molecular structure contains two moieties: an acetyl group and a peroxy group. This makes it both acidic and highly oxidizing. Peracetic acid has a high redox potential and serves various functions, including disinfection, bleaching, catalysis, and oxidation. It has been widely used as an oxidant to degrade organic pollutants, but its application in reducing strongly reducing substances in flooded rice paddies has not been reported. Summary of the Invention

[0006] The purpose of the present invention is to use peracetic acid as a strong reducing substance reducing agent to reduce the strong reducing substances in flooded rice fields after the application of organic fertilizers, reduce the negative impact of the accumulation of reducing substances on soil and rice, promote rice growth, and increase rice yield.

[0007] The present invention can be achieved through the following technical solutions:

[0008] The peracetic acid described in this invention is a commonly used disinfectant on the market and comes in two types: Liquid A and Liquid B. Liquid product specifications are: GB / T19001-2008 / ISO 9001:2008. As a further preferred embodiment of the present invention, the peracetic acid is prepared by mixing Liquid A and Liquid B in a 1:1 ratio, allowing the mixture to stand for 24 hours, and then diluting the mixture 100-1000 times with tap water.

[0009] The strong reducing substance reducing agent of the present invention is used in cultivating rice growth by adding different organic fertilizers to flooded rice fields.

[0010] Before transplanting rice seedlings, mix chemical and organic fertilizers evenly with the soil. Twenty days after transplanting, evenly sprinkle the diluted strong reducing agent into the soil. In potted plant trials, apply the agent twice at a rate of 1 ml (before dilution) per 1000 g of soil, 13 days apart. In outdoor and field trials, apply 2.3-2.5 L (before dilution) per mu of flooded rice field twice, 7 and 20 days after transplanting. Apply equal amounts twice.

[0011] The peracetic acid strong reducing substance reducing agent of the present invention is used to reduce reducing substances and increase rice yield in flooded rice fields where organic fertilizer is applied.

[0012] The application effects shown in the present invention are as follows:

[0013] The present invention has discovered a strong reducing substance reducing agent that can reduce the content of soil reducing substances, increase the soil redox potential, promote rice growth and increase yield after adding different organic fertilizers. In pot experiments. Compared with conventional chemical fertilizers, the application of organic fertilizers significantly increases the total amount of soil reducing substances and the content of active reducing substances, and significantly reduces the soil redox potential. Growth index measurements (plant height, fresh weight, dry weight) significantly decrease. After adding peracetic acid, the soil redox potential increases, the total amount of reducing substances and the content of active reducing substances significantly decrease, and growth indicators are significantly reflected. Field experiments show that the addition of peracetic acid effectively increases the soil redox potential and effectively promotes rice yield.

[0014] Compared with the prior art, the abatement agent used in the present invention has the following advantages:

[0015] The present invention discovers for the first time that peracetic acid can be used as a reducing agent to effectively reduce the total amount of strong reducing substances and the content of active reducing substances in flooded rice fields after the application of organic fertilizers, thereby increasing the soil redox potential, promoting rice growth, and increasing rice yield. Compared with a blank control (no peracetic acid added) and a negative control (calcium peroxide), peracetic acid significantly reduced the accumulation of reducing substances in the soil, increased the soil Eh to a range suitable for rice growth, and effectively promoted rice growth, demonstrating a superior effect on rice yield. BRIEF DESCRIPTION OF THE DRAWINGS

[0016] Figure 1 .Peracetic acid products and outdoor experimental design display.

[0017] Figure 2 .Effects of peracetic acid on rice growth in flooded pots with different organic fertilizers.

[0018] Figure 3 Effects of peracetic acid on soil reductive state in flooded pots with different organic fertilizers.

[0019] Figure 4 Effects of peracetic acid application on rice biomass in an outdoor flooding experiment with different organic fertilizers.

[0020] Figure 5 Effects of peracetic acid application on soil reducing state in an outdoor flooding experiment with different organic fertilizers.

[0021] Figure 6 Effects of peracetic acid application on rice yield in a field flooding experiment with the addition of different organic fertilizers.

[0022] Figure 7 .Effect of applying peracetic acid on soil reducing substances in a field flooding experiment with the addition of different organic fertilizers. Specific implementation plan

[0023] The following is a detailed description of the technical solution of the present invention in conjunction with preferred embodiments. The following embodiments are only used to illustrate and explain the present invention and do not constitute a limitation to the technical solution of the present invention.

[0024] Example 1: Effects of peracetic acid on rice growth and soil reducing substances in a flooded pot experiment with straw and organic fertilizer

[0025] Potted soil was collected from Jianchun Village, Zhiqian Town, Jintan City, Jiangsu Province (N31°39′, E119°28′), a long-term rice-wheat rotation experiment in which straw was returned to the field during the rice season. Soil was collected during the post-wheat harvest period in June 2023 during field irrigation and land preparation. The soil was brought back to the laboratory and dispensed into 12 cm (diameter) × 20 cm (height) pots, with 1000 g per pot. This method involves 7 treatments (T1 (chemical fertilizer control group), T2 (50% chemical fertilizer + straw organic fertilizer), T3 (50% chemical fertilizer + commercial organic fertilizer), T4 (50% chemical fertilizer + straw organic fertilizer + peracetic acid), T5 (50% chemical fertilizer + commercial organic fertilizer + peracetic acid), T6 (50% chemical fertilizer + straw organic fertilizer + calcium peroxide) and T7 (50% chemical fertilizer + commercial organic fertilizer + calcium peroxide). The specific fertilization scheme is shown in Table 1. Organic fertilizer was applied to the soil together with nitrogen fertilizer, phosphorus fertilizer and potassium fertilizer, mixed with water. Mix evenly. The artificially cultivated rice seedlings were transplanted into pots, with 3 plants per pot, the flooding water layer was maintained at 2 cm, and they were placed in an artificial climate chamber for cultivation (temperature 28°C, 12h light, 12h dark). Wheat straw was taken from the wheat stems and leaves (carbon-nitrogen ratio 17:1) after the wheat season harvest in the long-term positioning experiment of rice-wheat rotation in Jianchun Village, Zhiqian Town, Jintan City, Jiangsu Province (N31°39′, E119°28′). Urea was added to adjust the C / N ratio to 25:1, and then the straw organic fertilizer (N 2%, P2O51.6%, K2O 1.1%, OM≥30%) was formed after fermentation and decomposition for 3 months. The commercial organic fertilizer was produced by Nantong Huinong Bio-Organic Fertilizer Co., Ltd., with organic matter ≥30%, and total nutrients (N+P2O5+K2O) ≥4%. The peracetic acid used in the present invention is a commonly used disinfectant on the market, which is divided into liquid A and liquid B, as shown in the following figure. Figure 1 Product Specifications: GB / T19001-2008 / ISO 9001:2008. Peracetic acid solutions A and B were mixed in a 1:1 volume ratio, allowed to stand for 24 hours, then diluted 1000-fold with tap water. Seven days after transplanting, 500 ml of the diluted peracetic acid solution was added to the rice pots, with each pot containing 500 ml. Twenty days after transplanting, 500 ml of the diluted peracetic acid solution was added in the same manner. Calcium peroxide is a commonly used water purifier, with a dosage of 7.3 g per pot. Forty days after transplanting, soil redox potential (Eh) and dissolved oxygen content were measured using a YHBJ-262 soil ORP meter and a JPB-607A dissolved oxygen meter, respectively, manufactured by Shanghai Leici Instrument Co., Ltd. Soil samples were collected from 0 to 15 cm to determine soil physical and chemical properties and soil reducing substances. The total amount of soil reducing substances and the content of active reducing substances were determined using the aluminum sulfate extraction-potassium dichromate oxidation method and the 0.1 mol / L aluminum sulfate extraction-potassium manganate titration method, respectively. Rice plant height, dry weight, and fresh weight were recorded at 40 days of age, and the data were analyzed for variance using IBM SPSS 22.0.

[0026] The results are shown in Tables 2 and 3. Compared with T1 (chemical fertilizer control group), no significant differences were found in the various growth indicators of plants treated with T2 (50% chemical fertilizer + straw organic fertilizer) and T3 (50% chemical fertilizer + commercial organic fertilizer), indicating that the excessive amount of organic fertilizer replacing chemical fertilizer failed to promote rice growth. However, compared with the blank control group (T2 and T3 without added substances) and the negative control group (T6 and T7 with added calcium peroxide), the T4 (50% chemical fertilizer + straw organic fertilizer + peracetic acid) and T5 (50% chemical fertilizer + commercial organic fertilizer + peracetic acid) treatments significantly increased the dry weight, fresh weight and plant height of rice. Among them, the dry weight of the T4 and T5 treatments with added peracetic acid increased by 23.00% and 40.00% respectively compared with the T2 and T3 treatments, and the fresh weight increased by 49.46% and 43.41% respectively, indicating that the addition of peracetic acid promoted the growth of rice plants under the organic fertilizer application mode. Compared with the T2 and T3 treatments, no significant differences were found in plant height, dry weight and fresh weight of the T6 and T7 treatments of the negative control group (calcium peroxide), indicating that excessive addition of calcium oxide failed to promote rice growth. In Example 1, the highest soil redox potential (Eh) of 349 mV was observed in T1 (chemical fertilizer control group). In contrast, after adding a large amount of organic fertilizer, treatments T2 (50% chemical fertilizer + straw organic fertilizer) and T3 (50% chemical fertilizer + commercial organic fertilizer) showed extremely low Eh, both below 150 mV, indicating that the reducing property was extremely strong and not suitable for rice growth. The addition of peracetic acid significantly increased the soil Eh, both greater than 200 mV, which was suitable for rice growth. Compared with the T1 treatment, the T2 and T3 treatments produced a large amount of reducing substances. The total reducing substance content increased by 31.67% and 30.07% respectively compared with T1, and the active reducing substance content increased by 106.00% and 90.99% respectively. In contrast, treatments T5 and T6 significantly reduced the total amount and active reducing substances of the soil. Compared to treatments T2 and T3 without peracetic acid, the total amount of reducing substances decreased by 14.74% and 8.92%, respectively, and the active reducing substances decreased by 40.40% and 29.25%, respectively. While the negative control group showed significant decreases in the total amount and active reducing substances compared to the blank control (without added substances), no significant difference in Eh was observed. The results indicate that replacing half of the chemical fertilizer with organic fertilizer leads to the accumulation of reducing substances and fails to significantly promote rice growth. However, the addition of peracetic acid effectively alleviates the decrease in soil redox potential, improves soil quality, reduces reducing substances, and significantly promotes rice growth. Therefore, in this comparison, peracetic acid as the positive treatment outperformed T2 and T3 (blank controls) and T6 and T7 (negative controls), indicating that it is more effective in promoting rice growth.

[0027] Table 1 Fertilizer dosage and peracetic acid dosage for potted plant experiments (1000 g soil per pot, diluted 1000 times)

[0028]

[0029]

[0030] Table 2 Effects of peracetic acid on rice growth in flooded pots with different organic fertilizers

[0031] deal with Plant height (cm) Fresh weight (g) Dry weight (g) T1 48.59±1.76bc 5.18±1.39b 1.02±0.28c T2 45.64±0.93cd 4.63±0.75b 1.00±0.16c T3 44.60±1.13cd 4.86±1.02b 1.05±0.21bc T4 53.84±1.98ab 6.92±1.24a 1.23±0.11b T5 54.56±2.30a 6.97±1.18a 1.47±0.20b T6 41.75±14.74d 5.20±0.63b 1.01±0.15c T7 48.81±3.46abc 5.64±0.98b 1.09±0.20bc

[0032] Table 3 Effects of peracetic acid on soil reductive state (redox potential, total amount of reducing substances, active reducing substances) in flooded pots with different organic fertilizers

[0033] deal with Active reducing substances (cmol / L) Total amount of reducing substances (cmol / L) Redox potential (mV) T1 3.33±0.85d 19.99±2.81c 349.00±4.32a T2 6.86±0.48a 26.32±1.97ab 63.33±13.22d T3 6.36±0.97a 26.00±2.90ab 132.25±44.14c T4 4.02±0.78cd 22.44±2.21bc 288.00±46.25a T5 4.50±0.77bc 23.68±4.95bc 205.00±110.94b T6 5.15±0.82b 30.46±5.04a 55.50±31.40d T7 4.68±0.75bc 23.43±3.88bc 87.50±20.92cd

[0034] Example 2: Effects of Peracetic Acid on Rice Growth and Soil Reducing Substances in Outdoor Experiments

[0035] The outdoor experiment was conducted in Jiangning District, Nanjing, Jiangsu Province (N31°39′, E119°28′). This experiment involved seven treatments (T1 (chemical fertilizer control group), T2 (50% chemical fertilizer + straw organic fertilizer), T3 (50% chemical fertilizer + commercial organic fertilizer), T4 (50% chemical fertilizer + straw organic fertilizer + peracetic acid), T5 (50% chemical fertilizer + commercial organic fertilizer + peracetic acid), T6 (50% chemical fertilizer + straw organic fertilizer + calcium peroxide), and T7 (50% chemical fertilizer + commercial organic fertilizer + calcium peroxide). The specific fertilization scheme is shown in Table 4. Each sample was an embedded culture barrel with a diameter of 50 cm and an area of ​​0.789 m 2 , each pot of soil weighs 14kg. Figure 1 , each process 3 repetitions.Organic fertilizer is applied in the soil together with nitrogenous fertilizer, phosphate fertilizer and potash fertilizer, and mixed.Artificially cultivated rice seedlings are transplanted in basin, 4 strains per basin, and the flooded water layer keeps 5-10cm. The fertilizer application rate of each fertilization treatment is shown in Table 4, and wherein peracetic acid, chemical fertilizer, straw organic fertilizer and commercial organic fertilizer specification are with embodiment 1.It is summer rice (Nanjing 5055) that the rice quality that test plot adopts, and phosphate fertilizer, potash fertilizer and organic fertilizer are applied as base manure once, and nitrogenous fertilizer is applied in the form of base manure and topdressing, and base manure, tillering fertilizer, spike fertilizer are applied by 4:3:3. Peracetic acid solution A and solution B were mixed in a 1:1 volume ratio and allowed to stand for 24 hours. The mixture was then diluted 1000-fold and evenly spread on flooded soil 15 and 40 days after rice transplanting. The peracetic acid application rate was 0.014 L (before dilution) per pot, calculated as 1 ml of peracetic acid (before dilution) applied to 1000 g of soil. Soil redox potential, total amount of reducing substances, and active reducing substance content were determined as in Example 1. Calcium peroxide specifications were the same as in Example 1, with a dosage of 10 kg per mu. Tiller number, plant height, and fresh weight were recorded at rice maturity. Data were analyzed for variance using IBM SPSS 22.0.

[0036] The results are shown in Tables 5-6 and Figure 4As shown, the observation results of the outdoor experiment are consistent with those of the greenhouse pot experiment. Compared with T1 (chemical fertilizer control group), no significant differences were found in the growth indicators such as the number of effective panicles, plant height and fresh weight of the plants treated with T2 (50% chemical fertilizer + straw organic fertilizer) and T3 (50% chemical fertilizer + commercial organic fertilizer), indicating that organic fertilizer failed to promote the growth of rice instead of chemical fertilizer. However, treatments T4 (50% chemical fertilizer + straw organic fertilizer + peracetic acid) and T5 (50% chemical fertilizer + commercial organic fertilizer + peracetic acid) significantly increased the fresh weight of rice, plant height and the number of effective panicles compared with the blank control group (T2 and T3) and the negative control group (T6 and T7 with calcium peroxide). Among them, the fresh weight of T4 and T5 treated with peracetic acid increased by 50.19% and 157.02% respectively compared with the T2 and T3 treatments, indicating that the addition of peracetic acid promoted the growth of rice plants under the mode of applying organic fertilizer. The results of soil redox state are consistent with those in Example 1. Among all treatment groups, T1 (chemical fertilizer control group) had the highest soil redox potential (Eh), reaching 244.75mV. In contrast, T2 (50% chemical fertilizer + straw organic fertilizer) and T3 (50% chemical fertilizer + commercial organic fertilizer) showed significantly lower Eh, at -56.75mV and 22.25mV, respectively, indicating their strong reducing characteristics, which are not conducive to rice growth. Further analysis found that the soil Eh of T4 (50% chemical fertilizer + straw organic fertilizer + peracetic acid) and T5 (50% chemical fertilizer + commercial organic fertilizer + peracetic acid), which were added with peracetic acid, exceeded 200mV, indicating that they were suitable for rice growth. In addition, compared with T1, the content of reducing substances in the T2 and T3 treatment groups increased significantly, with total reducing substances increasing by 170.78% and 183.79%, respectively, and active reducing substances increasing by 106.38% and 265.96%, respectively. Treatments T5 and T6 significantly reduced the active reducing substances and the total amount of reducing substances. Compared with T2 and T3, which did not add peracetic acid, the total reducing substances decreased by 23.78% and 6.92%, and the active reducing substances decreased by 32.99% and 73.84%, respectively. Although the negative control groups (including T6 and T7) showed a significant decrease in the total amount of reducing substances and the active reducing substances compared with the blank controls (T2 and T3), the redox potential was lower. The results showed that although organic fertilizers replaced 50% of chemical fertilizers, they did not effectively promote rice growth. However, when peracetic acid combined with organic fertilizers replaced half of the chemical fertilizers, the redox potential of the soil was significantly increased, soil quality was improved, and reducing substances were reduced, thereby effectively promoting rice growth. Therefore, peracetic acid as a positive treatment showed a good promoting effect in all groups, better than T2, T3 (blank control group) and T6, T7 (negative control group). Table 4 Fertilizer application rate and peracetic acid dosage in outdoor experiments (each sample area is 0.789m 2， , each pot of soil weighs 14kg, diluted 1000 times)

[0037]

[0038] Table 5 Effects of peracetic acid on rice biomass in outdoor flooding experiments with different organic fertilizers

[0039] deal with Number of effective ears (per plant) Plant height (cm / plant) Fresh weight (g / plant) T1 25.50±5.96a 65.02±3.31a 115.91±31.74ab T2 25.00±7.38a 59.65±4.49a 94.03±32.82b T3 18.83±8.54a 58.52±8.06a 72.06±47.95b T4 25.33±3.88a 72.35±6.84a 141.23±44.62ab T5 30.17±17.24a 74.30±8.63a 185.21±120.55a T6 29.50±9.18a 63.12±5.79a 137.44±38.35ab T7 26.00±6.63a 65.02±29.06a 106.90±32.54ab

[0040] Table 6 Effects of peracetic acid on soil reducing state in outdoor flooding experiments with different organic fertilizers

[0041] deal with Active reducing substances (cmol / L) Total amount of reducing substances (cmol / L) Redox potential (mV) T1 0.47±0.17d 4.38±0.02c 244.75±16.09a T2 0.97±0.12b 11.86±0.21a -56.75±146.49c T3 1.72±0.37a 12.43±0.23a 22.25±68.84bc T4 0.65±0.12cd 9.04±2.22b 210.25±56.78a T5 0.45±0.11d 11.57±2.05a 219.50±23.13a T6 1.72±0.37a 8.29±0.15b 89.00±23.71b T7 0.79±0.09bc 8.62±0.31b 74.25±17.56b

[0042] Example 3: Effect of Peracetic Acid on Rice Yield and Soil Reducing Substances in Field Trials

[0043] The field experiment was conducted at Honghui Family Farm in Yazhou Town, Hai'an City, Jiangsu Province, involving 7 treatments (T1 (chemical fertilizer control group), T2 (50% chemical fertilizer + straw organic fertilizer), T3 (50% chemical fertilizer + commercial organic fertilizer), T4 (50% chemical fertilizer + straw organic fertilizer + peracetic acid), T5 (50% chemical fertilizer + commercial organic fertilizer + peracetic acid), T6 (50% chemical fertilizer + straw organic fertilizer + calcium peroxide) and T7 (50% chemical fertilizer + commercial organic fertilizer + calcium peroxide). The specific fertilization scheme is shown in Table 7 below. Each treatment is 0.23 mu, and the specifications of peracetic acid, chemical fertilizer, straw organic fertilizer and commercial organic fertilizer are the same as those in Example 1. The test area uses rice with a quality of For summer rice (Nanjing 5055), phosphate fertilizer, potash fertilizer, and organic fertilizer were applied as a single basal fertilizer, while nitrogen fertilizer was applied as a basal fertilizer and topdressing, with basal fertilizer, tillering fertilizer, and panicle fertilizer applied in a ratio of 4:3:3. Peracetic acid solutions A and B were mixed in a 1:1 ratio and allowed to stand for 24 hours. After dilution, the mixture was evenly spread on flooded soil 15 and 40 days after rice transplanting. The peracetic acid dosage after mixing was 2.5 L per mu (before dilution). Calcium peroxide specifications were the same as in Example 1, with a dosage of 10 kg per mu. Soil redox potential, total reducing substance content, and active reducing substance content were determined as in Example 1. Yield per mu was recorded at rice harvest. Data were analyzed for variance using IBM SPSS 22.0.

[0044] The results are shown in Tables 8 and 9. Compared with T1 (chemical fertilizer control group), there was no significant difference in the yield and growth indicators of T2 (50% chemical fertilizer + straw organic fertilizer) and T3 (50% chemical fertilizer + commercial organic fertilizer), which shows that excessive organic fertilizer replacing chemical fertilizer failed to increase production. However, T4 (50% chemical fertilizer + straw organic fertilizer + peracetic acid) and T5 (50% chemical fertilizer + commercial organic fertilizer + peracetic acid) were significantly better than T2 and T3 in terms of yield, effective panicle number and plant height. Specifically, the yields of T4 and T5 were 397.22kg / mu and 379.75kg / mu, respectively, which were increased by about 11.44% and 6.46% compared to 356.44kg / mu and 356.70kg / mu of T2 and T3, respectively. In addition, T4 and T5 also showed significant growth in effective panicle number and plant height, verifying the effectiveness of peracetic acid combined with organic fertilizer replacing half of the chemical fertilizer in increasing rice yield. Compared with T2 and T3, the negative control groups (T6 and T7) were not significantly better than the control group in these growth indicators, indicating that the addition of calcium oxide did not play a role in promoting rice growth. In soil testing, T1 (chemical fertilizer control group) showed the highest soil redox potential (Eh), which was 312.75mV. In contrast, treatments T2 (50% chemical fertilizer + straw organic fertilizer) and T3 (50% chemical fertilizer + commercial organic fertilizer) showed lower Eh due to the addition of large amounts of organic fertilizer, which was reduced to 50mV and even in the negative range, which is not conducive to rice growth. Treatments T4 (50% chemical fertilizer + straw organic fertilizer + peracetic acid) and T5 (50% chemical fertilizer + commercial organic fertilizer + peracetic acid) significantly increased soil Eh, both exceeding 200mV, which is within the range suitable for rice growth. Compared with T1, treatments T2 and T3 produced significantly more reducing substances, with total reducing substance content increasing by 43.59% and 106.41%, respectively, compared to T1; and active reducing substance content increased by 84.31% and 92.16%, respectively. In contrast, treatments T5 and T6 significantly reduced the total amount and activity of reducing substances, decreasing by 26.79% and 4.08%, respectively, compared to T2 and T3, and active reducing substances by 34.57% and 19.90%, respectively. Although the negative controls (T6 and T7) showed a significant decrease in active reducing substances compared to the blank control, Eh values ​​did not reach a range suitable for rice growth. These results suggest that replacing half of the chemical fertilizer with organic fertilizer leads to the accumulation of reducing substances and fails to significantly promote rice growth. However, the addition of peracetic acid effectively improved the soil redox potential, reduced the accumulation of reducing substances, and significantly promoted rice growth.

[0045] Table 3 Field experiment fertilizer amount and peracetic acid dosage (calculated per mu) (diluted 10 times)

[0046]

[0047] Table 8 Effects of peracetic acid on rice yield in field flooding experiments with different organic fertilizers

[0048]

[0049]

[0050] Table 9 Effects of peracetic acid on soil reducing substances (redox potential, total amount of reducing substances, active reducing substances) in field flooding experiments with different organic fertilizers

[0051] deal with Active reducing substances (cmol / L) Total amount of reducing substances (cmol / L) Redox potential (mV) T1 1.02±0.08d 5.46±0.85c 312.75±24.55d T2 1.88±0.28b 7.84±2.69bc -15.25±70.57b T3 1.96±0.26ab 11.27±2.20a 31.50±68.29ab T4 1.23±0.10d 5.74±1.28c 209.50±58.45d T5 1.57±0.31c 10.81±2.05a 282.25±34.31c T6 2.04±0.31ab 9.64±3.30ab 23.25±35.61ab T7 2.16±0.32a 8.78±3.01ab 14.50±30.69a

Claims

1. Application of peracetic acid as a strong reducing substance reducer in flooded rice fields with organic fertilizer.

2. The use according to claim 1, characterized in that The reducing agent can significantly increase the redox potential of the soil, reduce the content of reducing substances, and thus promote the growth and yield of rice.

3. Application of peracetic acid in reducing strong reducing substances in flooded rice fields after application of organic fertilizers.

4. The use according to claim 3, characterized in that Peracetic acid can significantly increase the soil redox potential of rice fields flooded with organic fertilizers, increase the soil dissolved oxygen content, and thus promote rice growth and yield.

5. The use according to claim 3, characterized in that The peracetic acid concentration is 0.15g / L-1.5g / L.

6. A method for reducing strong reducing substances in rice fields caused by returning straw to the fields and / or applying organic fertilizer, characterized in that: A peracetic acid solution with a concentration of 0.15 g / L to 1.5 g / L was applied to the flooded rice fields where organic fertilizer was applied.

7. The method according to claim 6, characterized in that The 0.15 g / L peracetic acid solution is prepared by the following method: commercially available peracetic acid solution A and solution B are mixed in a volume ratio of 1:1, allowed to stand for 24 hours, and diluted 100-1000 times with tap water.

8. The method according to claim 7, characterized in that The application amount of the peracetic acid is calculated based on the volume of the peracetic acid solution before dilution, and 0.8-1.2 ml of the peracetic acid solution before dilution is applied to every 1000 g of soil. The application is added twice, and the application time is 7 days and 20 days after transplanting the rice.

9. The method according to claim 7, characterized in that The application amount of the peracetic acid is calculated based on the volume of the peracetic acid solution before dilution, and 1 ml of the peracetic acid solution before dilution is applied to every 1000 g of soil. The application is added twice, and the application time is 7 days and 20 days after transplanting the rice.

10. The method according to claim 7, characterized in that The application amount of the reducing agent is calculated based on the volume of the peracetic acid solution before dilution, and 2.3 to 2.5 L of the peracetic acid solution before dilution is applied to each mu of flooded rice field. The application is added twice, and the application time is 7 days and 20 days after rice transplanting.

11. The method according to any one of claims 6 to 9, characterized in that By combining peracetic acid solution with the application of straw organic fertilizer or commercial organic fertilizer.

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