A microbial fermentation culture tank for planting culture medium

Abstract

This invention relates to the field of microbial culture technology, and more specifically, to a microbial fermentation culture tank for planting culture media. The tank includes a tank body, a motor fixedly mounted on the top of the tank cover, a stirring shaft fixedly connected to the bottom of the motor drive shaft, and a stirring paddle fixedly mounted on the outer side of the middle section of the stirring shaft. The invention is characterized by: a defoaming component positioned between the outer top of the stirring shaft and the inner wall of the tank; a scraper ring component positioned at the top of the defoaming component; and a homogenizing component positioned between the bottom of the defoaming component and the bottom of the stirring shaft. This invention uses a rotating stirring shaft to drive the defoaming paddle and collision blocks to rotate, causing the collision plates to collide with the collision blocks. This, in turn, causes the scraper ring to rotate and slide, scraping away the fermentation liquid on the inner wall. Simultaneously, the bottom stirring teeth can perform horizontal rotational stirring and linear reciprocating stirring perpendicular to the stirring shaft, resulting in a more uniform mixing density of the fermentation liquid at the bottom.

Description

Technical Field

[0001] This invention relates to the field of microbial culture technology, specifically to a microbial fermentation culture tank for planting culture medium. Background Technology

[0002] Microbial fermentation culture tanks (also known as bioreactors) are core equipment used for large-scale cultivation of microorganisms, cells or enzymes. Their basic working principle involves key aspects such as aseptic environment control, nutrient supply, environmental parameter adjustment and metabolite management.

[0003] The culture cycle of microorganisms in a fermenter can be roughly divided into a lag phase, a logarithmic growth phase, and a stationary phase. During the logarithmic growth phase, the metabolic activity of microorganisms is extremely vigorous, consuming large amounts of carbon sources (such as sugars) and nitrogen sources in the culture medium, and producing a large number of metabolites (such as proteins, polysaccharides, CO2, etc.). These metabolites (especially proteins) have surface activity, which reduces the surface tension of the culture medium and promotes foam formation. At the same time, the respiration of microorganisms releases CO2, which forms bubbles in the culture medium, further forming foam.

[0004] Excessive foam can cause fermentation broth to overflow from the top of the tank, resulting in product loss. Furthermore, a foam layer covering the liquid surface hinders oxygen transfer and reduces dissolved oxygen efficiency. Therefore, it is necessary to install a defoaming component inside the fermentation tank to quickly eliminate foam. Existing defoaming components are mostly of two types: one uses a rotating defoaming paddle, and the other uses ultrasound. Both methods have certain drawbacks.

[0005] When foam is generated, the expansion of the foam causes some of the fermentation liquid to adhere to the inner wall of the tank. During defoaming, the bursting of the foam also causes some of the fermentation liquid to splash onto the tank wall, resulting in the phenomenon of fermentation liquid sticking to the wall. Existing defoaming paddles and ultrasonic defoamers cannot reach the fermentation liquid sticking to the tank wall, and the height of the main fermentation liquid at the bottom of the tank is limited, making it impossible to entrain the fermentation liquid sticking to the wall for mixing, which will cause a certain amount of production waste.

[0006] Existing fermenters cannot further improve the mixing efficiency of the dense substances at the bottom of the fermentation liquid during defoaming. During the logarithmic growth phase when the inoculum produces foam, the inoculum produces a large amount of metabolites, which make the fermentation liquid thicker overall. When the metabolites settle to the bottom of the tank, they affect the overall density of the fermentation liquid. The unevenness of the fermentation liquid leads to a decrease in the efficiency of the inoculum in absorbing nutrients. Summary of the Invention

[0007] The purpose of this invention is to provide a microbial fermentation culture tank for planting culture medium that can physically defoam through collision, while simultaneously scraping off nutrients from the tank wall and making the density distribution of the fermentation liquid more uniform.

[0008] To achieve the above objectives, the present invention is implemented through the following technical solution: a microbial fermentation culture tank for planting culture medium, comprising a tank body, a motor fixedly installed on the top of the tank cover, a stirring shaft fixedly connected to the bottom of the motor drive shaft, and a stirring paddle fixedly installed on the outer side of the middle part of the stirring shaft. A defoaming component is provided between the outer side of the top of the stirring shaft and the inner wall of the tank body, a scraping ring component is provided at the bottom of the defoaming component, and a homogenizing component is provided between the bottom of the defoaming component and the bottom of the stirring shaft.

[0009] The defoaming assembly includes a defoaming paddle fixedly installed on the outer side of the top of the stirring shaft, a collision block fixedly connected to one end of the defoaming paddle, a rebound structure located on the inner wall of the tank, a slide rod located inside the rebound structure, and a collision plate fixedly connected to the top of the slide rod. A convex ball is fixedly connected to the top of the collision plate.

[0010] The scraper ring assembly includes a fixed ring that is fixedly connected to the outer side of the top of the slide bar and a scraper ring that is rotatably connected to the inner side of the outer ring of the fixed ring.

[0011] The homogenizing component includes a retaining ring fixedly connected to the bottom of the slide rod, a telescopic tube structure located on the outside of the bottom of the stirring shaft, and T-shaped stirring teeth located on the outside of the telescopic tube structure. The inner ring of the retaining ring is narrower at the top and wider at the bottom, and one end of the telescopic tube structure touches the inner ring of the retaining ring.

[0012] Furthermore, both the bottom end of the defoaming paddle and the bottom end of the collision block are fixedly connected with spike-shaped breaking teeth. The breaking teeth extend from the end where the defoaming paddle is connected to the stirring shaft towards the collision block, with the teeth decreasing in size and the spacing between the teeth increasing.

[0013] Furthermore, the collision plate and the collision block are slidably connected. The top of the collision plate is provided with a toothed groove, which meshes with the breaking teeth fixedly connected to the bottom of the collision block. The collision block and the breaking teeth at the bottom together form a lower semi-circular protrusion. When the collision block collides with the convex ball, the collision block presses down on the convex ball.

[0014] Furthermore, the rebound structure includes a support tube fixedly connected to the inner wall of the tank, a compression ring slidably connected to the inner side of the support tube, and a spring fixedly connected to the bottom end of the compression ring and the bottom end of the inner side of the support tube. The compression ring is fixedly connected to the outer side of the slide rod.

[0015] Furthermore, shaft seals are provided at the sliding connection points between the pipe ends of the support tube and the slide rod, and the slide rod slides inside the support tube, keeping the inside of the support tube sealed.

[0016] Furthermore, a spiral-shaped limiting strip is fixedly connected to the inner wall of the tank, and a limiting groove is provided on the outer side of the scraper ring to slide and connect with the limiting strip.

[0017] Furthermore, the telescopic tube structure includes a connecting rod fixedly installed on the outside of the bottom of the stirring shaft and a telescopic tube slidably connected to the outside of the connecting rod. The outside of the telescopic tube is fixedly connected to the stirring teeth. A pressing block is fixedly connected to the end of the connecting rod away from the stirring shaft. A spring is fixedly connected between the pressing block and the end of the telescopic tube on the inside away from the stirring shaft.

[0018] Furthermore, the inner wall of the telescopic tube is provided with a groove, and a protrusion is fixedly connected to the outer side of the extrusion block, with the protrusion slidingly connected to the groove.

[0019] Furthermore, a ball bearing is fitted inside the telescopic tube near the retaining ring, and the ball bearing is in rolling connection with the inner ring of the retaining ring.

[0020] Furthermore, a shaft seal is provided at the sliding connection between the telescopic tube opening and the connecting rod, allowing the telescopic tube to slide on the outside of the connecting rod while maintaining a seal on the inside of the telescopic tube.

[0021] This invention provides a microbial fermentation culture tank for planting culture media, which has the following beneficial effects:

[0022] (1) Based on the original defoaming paddle, the present invention sets up a defoaming component with dual effects. It can not only defoam by rotating the defoaming paddle, but also quickly impact and break up the foam that is waved to the inner edge of the tank by the defoaming paddle, thereby improving the breaking efficiency. At the same time, a scraping ring component is set on the outside of the defoaming component. When defoaming by impacting up and down, the fermentation liquid hanging on the inner wall of the tank can be scraped back into the main fermentation liquid, reducing production waste. Moreover, the scraping ring in the scraping ring component can rotate when scraping up and down, which can fling away the nutrients adhering to the scraping ring, thus having a certain self-cleaning function.

[0023] (2) The present invention provides a homogenizing component at the bottom of the defoaming component. The T-shaped stirring teeth of the homogenizing component can not only rotate horizontally under the drive of the stirring shaft, but also reciprocate linearly along the axis direction perpendicular to the stirring shaft under the drive of the defoaming component, so that the overall density of the fermentation liquid is uniform and the mixing efficiency of the high-density fermentation liquid is improved. Attached Figure Description

[0024] To more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings in the following description are merely exemplary, and those skilled in the art can derive other embodiments based on the provided drawings without creative effort.

[0025] Figure 1 This invention relates to a three-dimensional microbial fermentation culture tank for planting culture medium. Figure 1 .

[0026] Figure 2 This invention relates to a three-dimensional microbial fermentation culture tank for planting culture medium. Figure 2 .

[0027] Figure 3 This is a cross-sectional view of a microbial fermentation culture tank for planting culture medium according to the present invention.

[0028] Figure 4 This is a perspective view of the collision plate and defoaming paddle of the present invention.

[0029] Figure 5 This is the present invention. Figure 3 A magnified view of region A in the middle.

[0030] Figure 6 This is the present invention. Figure 3 A magnified view of region B in the middle.

[0031] Figure 7 This is the present invention. Figure 3 A magnified view of region C in the middle.

[0032] The diagram shows: 1. Tank body; 11. Cooling jacket; 12. Water inlet pipe; 13. Drain pipe; 14. Air inlet pipe; 15. Steam pipe; 16. Material discharge pipe; 2. Tank lid; 21. Exhaust pipe; 22. Culture medium filling tube; 23. Inoculum filling tube; 24. Observation window; 25. Temperature detector; 26. Dissolved oxygen detector; 27. pH detector; 3. Motor; 4. Stirring shaft; 5. Stirring paddle; 6. Defoaming assembly; 61. Defoaming paddle; 62. Collision block. 63. Slide bar; 64. Collision plate; 65. Convex ball; 7. Rebound structure; 71. Support tube; 72. Extrusion ring; 73. Spring one; 8. Scraper ring assembly; 81. Fixing ring; 82. Scraper ring; 83. Limiting strip; 84. Limiting groove; 9. Homogenizing assembly; 91. Retaining ring; 92. Stirring teeth; 10. Telescopic tube structure; 101. Connecting rod; 102. Telescopic tube; 103. Groove; 104. Extrusion block; 105. Spring two; 106. Ball bearing. Detailed Implementation

[0033] To make the above-mentioned objects, features, and advantages of the present invention more apparent and understandable, specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings. Many specific details are set forth in the following description to provide a thorough understanding of the present invention. However, the present invention can be implemented in many other ways different from those described below, and those skilled in the art can make similar modifications without departing from the spirit of the present invention. Therefore, the present invention is not limited to the specific embodiments disclosed below.

[0034] Please see Figure 1 , Figure 2 and Figure 3As shown, a microbial fermentation culture tank for planting culture medium includes a tank body 1, a tank cover 2 bolted to the top of the tank body 1, a motor 3 bolted to the top of the tank cover 2, a stirring shaft 4 fixedly connected to the bottom of the drive shaft of the motor 3, and a stirring paddle 5 bolted to the outer side of the middle of the stirring shaft 4. A sealing ring is provided at the connection between the tank body 1 and the tank cover 2 to maintain the internal sealing of the tank body 1, and a shaft seal is provided at the rotatable connection between the stirring shaft 4 and the tank cover 2 to maintain rotational sealing.

[0035] It should be noted that an exhaust pipe 21 is fixedly connected to one side of the top of the can lid 2, a culture medium filling tube 22 is provided on one side of the exhaust pipe 21, a microbial inoculum filling tube 23 is provided on one side of the culture medium filling tube 22, a temperature detector 25, a dissolved oxygen detector 26 and a pH detector 27 are installed on one side of the top of the can lid 2, and an observation window 24 is provided on the top of the other side of the can lid 2 opposite to the microbial inoculum filling tube 23.

[0036] The inner side of the tank body 1 is a fermentation chamber. The outer side of the tank body 1 is wrapped with a cooling jacket 11. A water inlet pipe 12 is provided at the bottom of one side of the cooling jacket 11, and a drain pipe 13 is provided at the top of the other side of the cooling jacket 11. An air inlet pipe 14 and a steam pipe 15 are respectively provided on both sides of the bottom end of the tank body 1. A discharge pipe 16 is fixedly connected to the center of the bottom end of the tank body 1.

[0037] As one embodiment of the present invention, before fermentation, the inside of the tank 1 needs to be disinfected. Steam at 121°C is filled into the tank 1 through the steam pipe 15 for high-temperature disinfection for minutes, and then discharged through the exhaust pipe 21. After disinfection, rapid cooling is performed by adding cooling water into the cooling jacket 11 through the water inlet pipe 12. After absorbing heat, the water is discharged through the drain pipe 13. The cooling jacket 11 can also maintain the inside of the tank 1 at a certain temperature.

[0038] The culture medium and inoculum are added into the tank 1 through the culture medium filling tube 22 and the inoculum filling tube 23, respectively. The culture begins after detection by the temperature detector 25, dissolved oxygen detector 26 and pH detector 27.

[0039] During the fermentation process, motor 3 can be started to drive the stirring shaft 4 to rotate, so that the stirring paddle 5 can stir the fermentation liquid and the inoculum and culture medium can be fully mixed.

[0040] Please see Figure 3 , Figure 4 , Figure 5 and Figure 6As shown, a defoaming assembly 6 is provided between the outer top of the stirring shaft 4 and the inner wall of the tank 1. The defoaming assembly 6 includes a defoaming paddle 61 fixedly installed on the outer top of the stirring shaft 4 by a collar and bolts, a collision block 62 fixedly connected to one end of the defoaming paddle 61, a rebound structure 7 provided on the inner wall of the tank 1, a slide rod 63 provided on the inner side of the rebound structure 7, and a collision plate 64 fixedly connected to the top of the slide rod 63. A convex ball 65 is fixedly connected to the top of the collision plate 64. Spiked breaking teeth are fixedly connected to the bottom of the defoaming paddle 61 and the bottom of the collision block 62. The breaking teeth extend from the end where the defoaming paddle 61 is connected to the stirring shaft 4 towards the collision block 62. The teeth are from large to small and the spacing between the teeth is from sparse to dense. The collision plate 64 is slidably connected to the collision block 62. The top of the collision plate 64 is provided with a toothed groove, which meshes with the breaking teeth fixedly connected to the bottom of the collision block 62. The collision block 62 and the breaking teeth at the bottom form a lower semi-circular protrusion. When the collision block 62 collides with the convex ball 65, the collision block 62 presses down on the convex ball 65.

[0041] The rebound structure 7 includes a support tube 71 fixedly connected to the inner wall of the tank 1, a compression ring 72 slidably connected to the inner side of the support tube 71, and a spring 73 fixedly connected to the bottom end of the compression ring 72 and the bottom end of the inner side of the support tube 71. The compression ring 72 is fixedly connected to the outer side of the slide rod 63. The two ends of the support tube 71 are provided with shaft seals at the sliding connection points with the slide rod 63. The slide rod 63 slides inside the support tube 71, and the inner side of the support tube 71 remains sealed.

[0042] As one embodiment of the present invention, when the stirring shaft 4 rotates, the stirring shaft 4 can drive the defoaming paddle 61, which is fixedly installed on its outer side by a collar and bolts, to rotate. The teeth of the breaking teeth at the bottom of the defoaming paddle 61 are from large to small and the tooth spacing is from sparse to dense. When rotating, the foam can be pushed between the collision plate 64 and the collision block 62 by centrifugal force. At the same time, the defoaming paddle 61 can also break some of the generated foam through the breaking teeth at its bottom.

[0043] When the collision block 62 strikes the downward-pressing convex ball 65, the collision plate 64 drives the slide rod 63 to move downward, causing the extrusion ring 72, which is fixedly welded to the outside of the slide rod 63, to press down the spring 73. When the collision block 62 moves away from the convex ball 65, the spring 73 quickly rebounds, pushing the extrusion ring 72 upward, causing the slide rod 63 to push the extrusion plate 64 upward. The extrusion plate 64 and the collision block 62 collide with each other, squeezing and breaking the foam trapped between them.

[0044] Please see Figure 3 , Figure 4 , Figure 5 and Figure 6As shown, the defoaming component 6 has a scraper ring assembly 8 at its bottom. The scraper ring assembly 8 includes a fixed ring 81 fixedly connected to the outer side of the top of the slide rod 63 and a scraper ring 82 rotatably connected to the inner side of the outer ring of the fixed ring 81. A spiral limiting strip 83 is fixedly connected to the inner wall of the tank body 1, and a limiting groove 84 is provided on the outer side of the scraper ring 82, which is slidably connected to the limiting strip 83.

[0045] In one embodiment of the present invention, when the slide bar 63 moves up and down, it drives the fixed ring 81 to move up and down. The fixed ring 81 drives the scraper ring 82 to move up and down, scraping the fermentation liquid with broken foam adhering to the inner wall of the tank 1 into the main fermentation liquid. During the up and down movement of the scraper ring 82, the spiral limiting strip 83 restricts the up and down movement of the scraper ring 82 through the limiting groove 84, so that the scraper ring 82 rotates 30° under the restriction of the limiting strip 83. As the scraper ring 82 moves up and down, the scraper ring 82 itself also rotates back and forth, throwing the fermentation liquid adhering to the scraper ring 82 into the main fermentation liquid.

[0046] Please see Figure 3 and Figure 7 As shown, a homogenizing component 9 is provided between the bottom end of the defoaming component 6 and the bottom of the stirring shaft 4. The homogenizing component 9 includes a retaining ring 91 fixedly connected to the bottom end of the slide rod 63, a telescopic tube structure 10 located on the outer side of the bottom of the stirring shaft 4, and T-shaped stirring teeth 92 located on the outer side of the telescopic tube structure 10. The inner ring of the retaining ring 91 is narrow at the top and wide at the bottom, and one end of the telescopic tube structure 10 touches the inner ring of the retaining ring 91.

[0047] The telescopic tube structure 10 includes a connecting rod 101 fixedly installed on the outer side of the bottom of the stirring shaft 4 by a collar and bolts, and a telescopic tube 102 slidably connected to the outer side of the connecting rod 101. The outer side of the telescopic tube 102 is fixedly connected to the stirring teeth 92. A pressing block 104 is fixedly connected to the end of the connecting rod 101 away from the stirring shaft 4. A spring 105 is fixedly connected between the pressing block 104 and the inner side of the telescopic tube 102 away from the stirring shaft 4.

[0048] The inner wall of the telescopic tube 102 is provided with a groove 103. A protrusion is fixedly connected to the outer side of the extrusion block 104. The protrusion is slidably connected to the groove 103. A ball bearing 106 is sleeved on the inner side of the end of the telescopic tube 102 near the retaining ring 91. The ball bearing 106 is slidably connected to the inner ring of the retaining ring 91. A shaft seal is provided at the sliding connection between the tube opening of the telescopic tube 102 and the connecting rod 101. The telescopic tube 102 slides on the outside of the connecting rod 101, and the inner side of the telescopic tube 102 remains sealed.

[0049] In one embodiment of the present invention, when the stirring shaft 4 rotates, the stirring shaft 4 can drive the connecting rod 101, which is fixedly installed on its outer side by a collar and bolts, to rotate horizontally, thereby driving the T-shaped stirring teeth 92 on the outer side of the telescopic tube 102 to rotate and stir the sediment at the bottom of the fermentation liquid.

[0050] When the slide bar 63 moves up and down, it causes the retaining ring 91 at the bottom to move up and down as well. Since the cross-section of the retaining ring 91 is narrower at the top and wider at the bottom, when the retaining ring 91 moves down, the distance between the retaining ring 91 and the stirring shaft 4 increases. The second spring 105 pushes the telescopic tube 102 closer to the retaining ring 91, so that the T-shaped stirring teeth 92 can move in a direction perpendicular to the stirring shaft 4. When the retaining ring 91 moves up, the distance between the retaining ring 91 and the stirring shaft 4 decreases. The retaining ring 91 pushes the telescopic tube 102 to contract through the ball bearing 106, compressing the second spring 105, so that the T-shaped stirring teeth 92 can move in the opposite direction perpendicular to the stirring shaft 4. This process repeats, so that the T-shaped stirring teeth 92 generate multi-directional stirring, reducing the density at the bottom of the fermentation liquid and making the overall density of the fermentation liquid more uniform.

[0051] In embodiments of the present invention, unless otherwise explicitly specified and limited, "above" or "below" the second feature can mean that the first feature is in direct contact with the second feature, or that the first feature is in indirect contact with the second feature through an intermediate medium. Furthermore, "above," "on top of," and "over" the second feature can mean that the first feature is directly above or diagonally above the second feature, or simply that the first feature is at a higher horizontal level than the second feature. "Below," "below," and "under" the second feature can mean that the first feature is directly below or diagonally below the second feature, or simply that the first feature is at a lower horizontal level than the second feature.

[0052] In the description of this invention, it should also be noted that, unless otherwise explicitly specified and limited, the terms "set," "connected," "installed," and "linked" should be interpreted broadly. For example, they can refer to a fixed connection, a detachable connection, an integral connection, or a sliding connection; they can refer to a mechanical connection or an electrical connection; they can refer to a direct connection or an indirect connection through an intermediate medium; and they can refer to the internal communication between two components. Those skilled in the art can understand the specific meaning of the above terms in this invention according to the specific circumstances.

[0053] The embodiments described herein are preferred embodiments of the present invention and are not intended to limit the scope of protection of the present invention. Therefore, all equivalent changes made based on the structure, shape, and principle of the present invention should be covered within the scope of protection of the present invention.

Claims

1. A microbial fermentation culture tank for planting culture medium, comprising a tank body, a motor fixedly installed on the top of the tank cover, a stirring shaft fixedly connected to the bottom end of the motor drive shaft, and a stirring paddle fixedly installed on the outer side of the middle part of the stirring shaft, characterized in that: A defoaming component is provided between the top outer side of the stirring shaft and the inner wall of the tank. A scraper ring component is provided at the bottom of the defoaming component. A homogenizing component is provided between the bottom of the defoaming component and the bottom of the stirring shaft. The defoaming assembly includes a defoaming paddle fixedly installed on the outer side of the top of the stirring shaft, a collision block fixedly connected to one end of the defoaming paddle, a rebound structure provided on the inner wall of the tank, a slide rod provided on the inner side of the rebound structure, and a collision plate fixedly connected to the top of the slide rod. A convex ball is fixedly connected to the top of the collision plate. The scraper ring assembly includes a fixed ring that is fixedly connected to the outer side of the top of the slide bar and a scraper ring that is rotatably connected to the inner side of the outer ring of the fixed ring; The homogenizing component includes a retaining ring fixedly connected to the bottom of the slide rod, a telescopic tube structure located on the outside of the bottom of the stirring shaft, and T-shaped stirring teeth located on the outside of the telescopic tube structure. The inner ring of the retaining ring is narrower at the top and wider at the bottom, and one end of the telescopic tube structure touches the inner ring of the retaining ring. Both the bottom of the defoaming paddle and the bottom of the collision block are fixedly connected with spike-shaped breaking teeth. The breaking teeth extend from the end where the defoaming paddle is connected to the stirring shaft towards the collision block. The teeth are from large to small and the spacing between the teeth is from sparse to dense. The collision plate and the collision block are slidably connected. The top of the collision plate has a toothed groove, which meshes with the breaking teeth fixedly connected to the bottom of the collision block. The collision block and the breaking teeth at the bottom form a lower semi-circular protrusion. When the collision block collides with the convex ball, the collision block presses down on the convex ball.

2. The microbial fermentation culture tank for planting culture medium according to claim 1, characterized in that: The spring-loaded structure includes a support tube fixedly connected to the inner wall of the tank, a compression ring slidably connected to the inner side of the support tube, and a spring fixedly connected to the bottom end of the compression ring and the bottom end of the inner side of the support tube. The compression ring is fixedly connected to the outer side of the slide rod.

3. The microbial fermentation culture tank for planting culture medium according to claim 2, characterized in that: Shaft seals are provided at the sliding connection points between the pipe ends of the support tube and the slide rod. The slide rod slides inside the support tube, and the inside of the support tube remains sealed.

4. The microbial fermentation culture tank for planting culture medium according to claim 1, characterized in that: A spiral-shaped limiting strip is fixedly connected to the inner wall of the tank, and a limiting groove is opened on the outer side of the scraper ring to slide and connect with the limiting strip.

5. The microbial fermentation culture tank for planting culture medium according to claim 1, characterized in that: The telescopic tube structure includes a connecting rod fixedly installed on the outside of the bottom of the stirring shaft and a telescopic tube slidably connected to the outside of the connecting rod. The outside of the telescopic tube is fixedly connected to the stirring teeth. A pressing block is fixedly connected to the end of the connecting rod away from the stirring shaft. A spring is fixedly connected between the pressing block and the end of the telescopic tube away from the stirring shaft.

6. The microbial fermentation culture tank for planting culture medium according to claim 5, characterized in that: The inner wall of the telescopic tube has a groove, and a protrusion is fixedly connected to the outside of the extrusion block. The protrusion is slidably connected to the groove.

7. A microbial fermentation culture tank for planting culture medium according to claim 5, characterized in that: A ball bearing is fitted inside the telescopic tube near the retaining ring, and the ball bearing is in rolling contact with the inner ring of the retaining ring.

8. The microbial fermentation culture tank for planting culture medium according to claim 5, characterized in that: The telescopic tube opening is equipped with a shaft seal at the sliding connection between the connecting rod and the tube opening. The telescopic tube slides on the outside of the connecting rod while the inside of the telescopic tube remains sealed.

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