Codonopsis pilosula stock solution and preparation method thereof

Abstract

The invention discloses a pilose asiabell root stock solution and a preparation method thereof. The invention belongs to the technical field of traditional Chinese medicine extraction and processing. The pilose asiabell root beverage comprises pilose asiabell root blocks, purified water, a compound enzyme preparation, sodium ascorbate and honey. By optimizing the raw material ratio and adopting a low-temperature dynamic extraction process and a compound enzyme preparation assisted extraction technology, the extraction rate of active ingredients is remarkably increased, and heat-sensitive ingredients are reserved. The preparation method comprises the steps of enzymolysis, enzyme deactivation, antioxidant treatment, filtration, low-temperature vacuum concentration and the like. The method can efficiently extract the active ingredients of the pilose asiabell root, avoids high-temperature damage and auxiliary material addition, meets the requirements of no additive and pure nature, improves the product stability, is suitable for industrial production, and solves the problems of low retention rate of the active ingredients, poor stability and difficulty in large-scale production in the traditional process.

Description

Technical Field

[0001] The invention belongs to the technical field of traditional Chinese medicine extraction and processing, and specifically relates to a Codonopsis pilosula stock solution and a preparation method thereof. Background Art

[0002] Codonopsis pilosula (Dongshen) is a perennial herbaceous plant of the genus Codonopsis in the Campanulaceae family. It is a high-quality variety of Codonopsis pilosula, primarily produced in Wen County, Gansu Province. Rich in active ingredients such as polysaccharides, saponins, amino acids, and trace elements, it boasts medicinal and health benefits such as tonifying Qi, strengthening the spleen and lungs, and enhancing immunity. It holds high application value in both Traditional Chinese Medicine and health food applications. In recent years, with the growing demand for natural health products, the development of products related to Codonopsis pilosula (Dongshen) has garnered significant attention.

[0003] Currently, the main processing forms of Codonopsis pilosula include dried herbs, slices, powder, and oral liquid. Dried herbs and slices are often used in traditional decoctions, which can lead to insufficient dissolution of active ingredients, low absorption efficiency, and inconvenience. While powdered Codonopsis pilosula can be taken directly, it is prone to clumping, and some heat-sensitive active ingredients may be lost due to high temperatures or oxidation during the grinding process. To improve taste and shelf life, oral liquid Codonopsis pilosula products often contain additives such as sucrose, preservatives, and flavorings. This not only affects the natural flavor and efficacy of the herb, but also fails to meet modern consumers' demand for "additive-free," "purely natural" health products.

[0004] In addition, the existing preparation process of Codonopsis pilosula liquid products mostly adopts a single water boiling extraction or ethanol extraction method. Although water boiling extraction is simple to operate, it can easily lead to the destruction of heat-sensitive active ingredients (such as some saponins and amino acids), and the impurity content in the extract is relatively high, affecting the purity of the product; although ethanol extraction can increase the extraction rate of some fat-soluble components, the subsequent ethanol removal process is complicated and there may be safety hazards such as residual organic solvents. At the same time, the existing process is not precise enough in controlling key parameters such as extraction temperature, time, and material-liquid ratio, resulting in large fluctuations in the content of active ingredients in different batches of products, poor stability, and difficulty in achieving industrial-scale production.

[0005] Therefore, developing a Codonopsis pilosula concentrate and a preparation method thereof that can maximize the retention of the active ingredients of Codonopsis pilosula, has no additional additives, is highly stable and is suitable for industrial production has become an urgent problem to be solved. Summary of the Invention

[0006] The purpose of the present invention is to provide a Codonopsis pilosula stock solution and a preparation method thereof. The present invention introduces a composite enzyme preparation and utilizes the synergistic effect of cellulase and pectinase to effectively degrade the cell wall structure of Codonopsis pilosula, promote the dissolution of active ingredients such as polysaccharides, saponins and amino acids, and significantly improve the extraction efficiency; at the same time, a low-temperature dynamic extraction process is adopted, and the temperature of the entire extraction process is controlled below 70°C, avoiding the destruction of heat-sensitive active ingredients (such as some saponins and amino acids) by high temperature, and retaining the natural efficacy of Codonopsis pilosula to the maximum extent; the present invention improves the stability of Codonopsis pilosula stock solution from the chemical and physical levels respectively by adding sodium ascorbate and honey. Sodium ascorbate inhibits the oxidation and deterioration of active ingredients, while honey forms a stable dispersion system through its natural sugar components, extending the shelf life of the product; the preparation method of the present invention has a simple process, clear and controllable parameters, and each step is suitable for large-scale mechanized operation, which can realize the industrial-scale production of Codonopsis pilosula stock solution and solve the problem of large batch differences in traditional processes.

[0007] In order to achieve the above-mentioned purpose, the technical solution adopted by the present invention is as follows: a Codonopsis pilosula stock solution and a preparation method thereof, wherein the Codonopsis pilosula stock solution comprises the following components in parts by weight: 50-200 parts of Codonopsis pilosula blocks, 200-600 parts of purified water, 0.2-4 parts of a complex enzyme preparation, 0.1-1 parts of sodium ascorbate, and 4-20 parts of honey.

[0008] Preferably, the Codonopsis pilosula stock solution comprises the following components in parts by weight: 100-150 parts of Codonopsis pilosula blocks, 300-500 parts of purified water, 0.5-2 parts of complex enzyme preparation, 0.1-0.5 parts of sodium ascorbate, and 5-10 parts of honey.

[0009] Preferably, the complex enzyme preparation is composed of a mixture of cellulase and pectinase in a mass ratio of 1:1-2, wherein the cellulase is used to degrade the cellulose structure in the cell wall of Codonopsis pilosula, and the pectinase is used to decompose the pectin substance in the cell wall, thereby promoting the dissolution of active ingredients such as polysaccharides, saponins and amino acids in Codonopsis pilosula.

[0010] Preferably, the sodium ascorbate is used as an antioxidant to inhibit the deterioration of the active ingredients of Codonopsis pilosula due to oxidation during the extraction process, while maintaining the stability of the extract.

[0011] Preferably, the honey is used to adjust the taste of the Codonopsis pilosula stock solution, and at the same time, the natural sugar components in the honey can form a stable dispersion system with the Codonopsis pilosula extract, further improving the physical stability of the stock solution.

[0012] The present invention also provides a method for preparing a Codonopsis pilosula stock solution, the method comprising the following steps:

[0013] (1) Mixing the Codonopsis pilosula block with purified water in proportion, adding a composite enzyme preparation, and performing enzymatic hydrolysis for 2-4 hours at a temperature of 40-50° C. and a stirring speed of 100-200 rpm to obtain an enzymatic hydrolyzate;

[0014] (2) heating the enzymatic hydrolysate to 60-70°C, maintaining the temperature for 10-20 minutes to inactivate the enzyme preparation, and then cooling the solution to room temperature;

[0015] (3) adding sodium ascorbate to the cooled enzymatic hydrolyzate, stirring evenly, and then standing for 1-2 hours to obtain a stable solution;

[0016] (4) filtering the stabilized solution through a microporous filter membrane to remove insoluble impurities and obtain a clarified solution;

[0017] (5) Add honey to the clarified liquid, stir evenly, and then perform low-temperature vacuum concentration, controlling the concentration temperature to 50-60° C. and the vacuum degree to -0.08 MPa, until the solid content of the concentrate reaches 20-30%, thereby obtaining the Codonopsis pilosula stock solution.

[0018] Preferably, the temperature of the enzymatic hydrolysis treatment in step (1) is 45° C. and the stirring speed is 150 rpm.

[0019] Preferably, the pore size of the microporous filter membrane in step (4) is 0.45 μm.

[0020] The beneficial effects achieved by the above structure are as follows: (1) The present invention introduces a composite enzyme preparation and utilizes the synergistic effect of cellulase and pectinase to effectively degrade the cell wall structure of Codonopsis pilosula, promote the dissolution of active ingredients such as polysaccharides, saponins and amino acids, and significantly improve the extraction efficiency; (2) The present invention adopts a low-temperature dynamic extraction process, and the temperature of the entire extraction process is controlled below 70°C, thereby avoiding the damage of heat-sensitive active ingredients (such as some saponins and amino acids) caused by high temperature, and retaining the natural efficacy of Codonopsis pilosula to the greatest extent; (3) The present invention improves the stability of Codonopsis pilosula stock solution from the chemical and physical levels by adding sodium ascorbate and honey. Sodium ascorbate inhibits the oxidation and deterioration of the active ingredients, while honey forms a stable dispersion system through its natural sugar components, thereby extending the shelf life of the product; (4) The preparation method of the present invention has a simple process, clear and controllable parameters, and each step is suitable for large-scale mechanized operation, which can realize the industrial-scale production of Codonopsis pilosula stock solution and solve the problem of large batch differences in traditional processes. BRIEF DESCRIPTION OF THE DRAWINGS

[0021] Figure 1 Effects of a Codonopsis pilosula stock solution and its preparation method on the total bacterial count.

[0022] The accompanying drawings are used to provide further understanding of the present invention and constitute a part of the specification. They are used to explain the present invention together with the embodiments of the present invention and do not constitute a limitation of the present invention. DETAILED DESCRIPTION

[0023] The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, rather than all the embodiments; based on the embodiments of the present invention, all other embodiments obtained by ordinary technicians in this field without making creative work are within the scope of protection of the present invention.

[0024] Unless otherwise defined, all technical and scientific terms used herein have the same meanings as those familiar to those skilled in the art. Furthermore, any methods and materials similar or equivalent to those described herein can be applied to the present invention. The preferred embodiments and materials described herein are for illustrative purposes only and are not intended to limit the scope of this application.

[0025] The experimental methods in the following examples, unless otherwise specified, are conventional methods; the test materials and test strains used in the following examples, unless otherwise specified, are purchased from commercial channels.

[0026] Example 1

[0027] A kind of Codonopsis pilosula stock solution and preparation method thereof

[0028] The Codonopsis pilosula stock solution comprises the following components in parts by weight: 100 parts of Codonopsis pilosula blocks, 500 parts of purified water, 0.5 parts of complex enzyme preparation, 0.5 parts of sodium ascorbate, and 5 parts of honey.

[0029] The complex enzyme preparation is composed of a mixture of cellulase and pectinase in a mass ratio of 1:1-2, wherein the cellulase is used to degrade the cellulose structure in the cell wall of Codonopsis pilosula, and the pectinase is used to decompose the pectin substance in the cell wall, thereby promoting the dissolution of active ingredients such as polysaccharides, saponins and amino acids inside Codonopsis pilosula.

[0030] The sodium ascorbate acts as an antioxidant, which can inhibit the deterioration of the active ingredients of Codonopsis pilosula caused by oxidation during the extraction process, while maintaining the stability of the extract.

[0031] The honey is used to adjust the taste of the Codonopsis pilosula raw liquid. At the same time, the natural sugar components in the honey can form a stable dispersion system with the Codonopsis pilosula extract, further improving the physical stability of the raw liquid.

[0032] The present invention also provides a method for preparing a Codonopsis pilosula stock solution, the method comprising the following steps:

[0033] (1) Mixing the Codonopsis pilosula block with purified water in proportion, adding a composite enzyme preparation, and performing enzymatic hydrolysis for 2-4 hours at a temperature of 40-50° C. and a stirring speed of 100-200 rpm to obtain an enzymatic hydrolyzate;

[0034] (2) heating the enzymatic hydrolysate to 60-70°C, maintaining the temperature for 10-20 minutes to inactivate the enzyme preparation, and then cooling the solution to room temperature;

[0035] (3) adding sodium ascorbate to the cooled enzymatic hydrolyzate, stirring evenly, and then standing for 1-2 hours to obtain a stable solution;

[0036] (4) filtering the stabilized solution through a microporous filter membrane to remove insoluble impurities and obtain a clarified solution;

[0037] (5) Add honey to the clarified liquid, stir evenly, and then perform low-temperature vacuum concentration, controlling the concentration temperature to 50-60° C. and the vacuum degree to -0.08 MPa, until the solid content of the concentrate reaches 20-30%, thereby obtaining the Codonopsis pilosula stock solution.

[0038] The temperature of the enzymatic hydrolysis treatment in step (1) is 45° C. and the stirring speed is 150 rpm.

[0039] Wherein, the pore size of the microporous filter membrane in step (4) is 0.45 μm.

[0040] Example 2

[0041] A kind of Codonopsis pilosula stock solution and preparation method thereof

[0042] The Codonopsis pilosula stock solution comprises the following components in parts by weight: 150 parts of Codonopsis pilosula blocks, 300 parts of purified water, 2 parts of complex enzyme preparation, 0.1 part of sodium ascorbate, and 10 parts of honey.

[0043] The complex enzyme preparation is composed of a mixture of cellulase and pectinase in a mass ratio of 1:1-2, wherein the cellulase is used to degrade the cellulose structure in the cell wall of Codonopsis pilosula, and the pectinase is used to decompose the pectin substance in the cell wall, thereby promoting the dissolution of active ingredients such as polysaccharides, saponins and amino acids inside Codonopsis pilosula.

[0044] The sodium ascorbate acts as an antioxidant, which can inhibit the deterioration of the active ingredients of Codonopsis pilosula caused by oxidation during the extraction process, while maintaining the stability of the extract.

[0045] The honey is used to adjust the taste of the Codonopsis pilosula raw liquid. At the same time, the natural sugar components in the honey can form a stable dispersion system with the Codonopsis pilosula extract, further improving the physical stability of the raw liquid.

[0046] The present invention also provides a method for preparing a Codonopsis pilosula stock solution, and the preparation method is carried out with reference to Example 1.

[0047] Example 3

[0048] A kind of Codonopsis pilosula stock solution and preparation method thereof

[0049] The Codonopsis pilosula stock solution comprises the following components in parts by weight: 125 parts of Codonopsis pilosula blocks, 400 parts of purified water, 1.2 parts of complex enzyme preparation, 0.3 parts of sodium ascorbate, and 8 parts of honey.

[0050] The complex enzyme preparation is composed of a mixture of cellulase and pectinase in a mass ratio of 1:1-2, wherein the cellulase is used to degrade the cellulose structure in the cell wall of Codonopsis pilosula, and the pectinase is used to decompose the pectin substance in the cell wall, thereby promoting the dissolution of active ingredients such as polysaccharides, saponins and amino acids inside Codonopsis pilosula.

[0051] The sodium ascorbate acts as an antioxidant, which can inhibit the deterioration of the active ingredients of Codonopsis pilosula caused by oxidation during the extraction process, while maintaining the stability of the extract.

[0052] The honey is used to adjust the taste of the Codonopsis pilosula raw liquid. At the same time, the natural sugar components in the honey can form a stable dispersion system with the Codonopsis pilosula extract, further improving the physical stability of the raw liquid.

[0053] The present invention also provides a method for preparing a Codonopsis pilosula stock solution, and the preparation method is carried out with reference to Example 1.

[0054] Test Example 1

[0055] A Codonopsis pilosula stock solution and its preparation method and its effect on stability

[0056] The Codonopsis pilosula stock solutions prepared in Examples 1-3 of the present invention were used as test samples and designated as Example 1, Example 2, and Example 3. A control group used a commercially available Codonopsis pilosula stock solution, with three replicates per group. Each group was placed in a dark environment at room temperature (25°C ± 5°C). After 12 months, samples were collected and the total microbial count was determined using the GB 4789.2 standard.

[0057] Result analysis: Figure 1 As shown, the stability of Examples 1-3 groups was excellent under normal temperature conditions, and the microorganisms met the standards, among which the performance of Example 3 group was the best; the stability of the control group was poor, and the total number of microbial colonies exceeded the standard.

[0058] While embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that various changes, modifications, substitutions, and variations may be made to these embodiments without departing from the principles and spirit of the invention, and that the scope of the invention is defined by the appended claims and their equivalents.

[0059] The present invention and its embodiments are described above. This description is not restrictive. The drawings show only one embodiment of the present invention, and the actual structure is not limited thereto. In short, if a person skilled in the art is inspired by this and, without departing from the purpose of the present invention, designs structures and embodiments similar to this technical solution without inventiveness, they shall fall within the scope of protection of the present invention.

Claims

1. A Codonopsis pilosula stock solution, characterized by: The Codonopsis pilosula stock solution comprises the following components in parts by weight: 50-200 parts of Codonopsis pilosula blocks, 200-600 parts of purified water, 0.2-4 parts of complex enzyme preparation, 0.1-1 parts of sodium ascorbate, and 4-20 parts of honey.

2. The Codonopsis pilosula stock solution according to claim 1, characterized in that: The Codonopsis pilosula stock solution comprises the following components in parts by weight: 100-150 parts of Codonopsis pilosula blocks, 300-500 parts of purified water, 0.5-2 parts of complex enzyme preparation, 0.1-0.5 parts of sodium ascorbate, and 5-10 parts of honey.

3. The Codonopsis pilosula stock solution according to claim 2, characterized in that: The complex enzyme preparation is prepared by mixing cellulase and pectinase in a mass ratio of 1:1-2, wherein the cellulase is used to degrade the cellulose structure in the cell wall of Codonopsis pilosula, and the pectinase is used to decompose the pectin substance in the cell wall, thereby promoting the dissolution of active ingredients such as polysaccharides, saponins and amino acids inside Codonopsis pilosula.

4. The Codonopsis pilosula stock solution according to claim 3, characterized in that: The sodium ascorbate acts as an antioxidant to inhibit the deterioration of the active ingredients of Codonopsis pilosula due to oxidation during the extraction process, while maintaining the stability of the extract.

5. The Codonopsis pilosula stock solution according to claim 4, characterized in that: The honey is used to adjust the taste of the Codonopsis pilosula raw liquid. At the same time, the natural sugar components in the honey can form a stable dispersion system with the Codonopsis pilosula extract, further improving the physical stability of the raw liquid.

6. A method for preparing the Codonopsis pilosula stock solution according to claim 5, characterized in that: The preparation method comprises the following steps: (1) Mixing the Codonopsis pilosula block with purified water in proportion, adding a composite enzyme preparation, and performing enzymatic hydrolysis for 2-4 hours at a temperature of 40-50° C. and a stirring speed of 100-200 rpm to obtain an enzymatic hydrolyzate; (2) heating the enzymatic hydrolysate to 60-70°C, maintaining the temperature for 10-20 minutes to inactivate the enzyme preparation, and then cooling the solution to room temperature; (3) adding sodium ascorbate to the cooled enzymatic hydrolyzate, stirring evenly, and then standing for 1-2 hours to obtain a stable solution; (4) filtering the stabilized solution through a microporous filter membrane to remove insoluble impurities and obtain a clarified solution; (5) Add honey to the clarified liquid, stir evenly, and then perform low-temperature vacuum concentration, controlling the concentration temperature to 50-60° C. and the vacuum degree to -0.08 MPa, until the solid content of the concentrate reaches 20-30%, thereby obtaining the Codonopsis pilosula stock solution.

7. The method for preparing a Codonopsis pilosula stock solution according to claim 6, characterized in that: The temperature of the enzymatic hydrolysis treatment in step (1) is 45° C. and the stirring speed is 150 rpm.

8. The method for preparing a Codonopsis pilosula stock solution according to claim 7, characterized in that: The pore size of the microporous filter membrane in step (4) is 0.45 μm.

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