Spore screening and collecting device after solid fermentation of biopesticide

Abstract

The invention relates to the technical field of biological processing, and particularly discloses a screening and collecting device for spores after solid fermentation of biopesticide, comprising: a first box body, the top of which is provided with a sealing top cover and is internally provided with a multi-stage sieve plate; the gas temperature control purging assembly is communicated with the first box body and is used for introducing heating protective gas into the first box body, directly destroying the adhesive force between spores and a substrate (such as bran / rice husk) by directionally purging solid fermentation materials, weakening a water strengthening effect by utilizing a drying effect and eliminating spore residues; the second box body communicates with the bottom of the first box body and is internally provided with a cloth bag and the side wall is connected with an exhaust assembly; the vibration assembly is mounted at the bottom of the second box body; the control host is electrically connected with the gas temperature control purging assembly, the exhaust assembly and the vibration assembly; the second box body is arranged at the bottom of the second box body; the control host optimizes the whole spore separation process by integrally regulating and controlling the gas flow rate, the negative pressure intensity and the vibration parameters, and the yield, the production economy and the equipment reliability are remarkably improved.

Description

Technical Field

[0001] The present application relates to the field of bioprocessing technology, and in particular to a spore screening and collection device after solid fermentation of biological pesticides. Background Art

[0002] Biopesticides are formulations that use living organisms (such as fungi, bacteria, insect viruses, genetically modified organisms, and natural enemies) or their metabolites to kill or inhibit agricultural pests. These pesticides are safe, environmentally friendly, and pollution-free, making them a crucial tool for promoting green and high-quality agricultural development. They align with modern agricultural trends and are increasingly favored by farmers.

[0003] When using bacterial or fungal spores to prepare biological pesticides, deep liquid fermentation or solid fermentation is usually used for production because the spores have tiny particle size and fine powder appearance.

[0004] Submerged liquid fermentation is mainly used for bacterial biopesticides (such as Bacillus). This process has simple equipment, mature technology, and controllable process, but it has the disadvantages of high fermentation costs and large wastewater production.

[0005] Solid-state fermentation is applicable to the production of fungal biopesticides (such as Paecilomyces lilacinus, Beauveria bassiana, Metarhizium anisopliae, and Trichoderma). Its core process involves the growth of microorganisms on a solid substrate. During fermentation, the substrate's moisture gradually evaporates, and the fungi transition from the mycelial growth phase to the reproductive phase, producing large quantities of spores. This process offers significant advantages: the solid substrate can be recycled or converted into fertilizer, achieving zero waste emissions. Furthermore, it requires minimal equipment investment, low fermentation costs, and is easily scalable.

[0006] After solid fermentation, the mycelium is tightly attached to the substrate and difficult to fall off, while the spores are easily separated from the mycelium. Currently, screening is the main method used to separate and collect spores from solid fermentation products. However, existing vibration screening equipment has key technical defects:

[0007] The spores in the fermentation product are tightly bound to the substrate (e.g., spores adhere to the surface of bran / rice husks). Residual moisture in the material further strengthens the binding force, making it difficult for existing equipment to effectively separate them. This results in a large amount of spores remaining in the substrate and a low spore yield (resulting in product loss and increased costs).

[0008] Fungal spores are fine and light, making them difficult to screen efficiently by gravity. During the screening process, a large number of spores float upward, which not only prolongs the screening time but also further reduces the yield. Summary of the Invention

[0009] To achieve the purpose of this application—to resolve the industry bottleneck of high spore residual rate and large drift loss—this application provides a spore screening and collection device after solid fermentation of biopesticides, comprising:

[0010] The first box body has a sealed top cover on the top and a multi-stage sieve plate arranged inside;

[0011] a gas temperature-controlled purge assembly, connected to the first box, for introducing heated gas into the box to purge the solid fermentation material;

[0012] The second box body is connected to the bottom of the first box body, has a cloth bag inside, and its side wall is connected to the exhaust assembly;

[0013] a vibration assembly, mounted on the bottom of the second box;

[0014] The control host is electrically connected to the gas temperature control purge component, the exhaust component and the vibration component respectively.

[0015] Furthermore, an ultrasonic generator probe is provided in the first box body, the probe is located in the upper chamber of the first-stage sieve plate, and is electrically connected to the control host.

[0016] Furthermore, the sieve hole diameters of the multi-stage sieve plates decrease step by step from top to bottom.

[0017] Furthermore, the gas temperature-controlled purge assembly includes:

[0018] A nitrogen generator, the gas outlet of which is connected to the gas heating chamber;

[0019] A gas heating chamber, wherein an electric heating element is provided inside the gas heating chamber, wherein the gas outlet end of the gas heating chamber is connected to the gas inlet end of the first electronic control valve and the gas inlet end of the second electronic control valve in two ways through the gas inlet pipe;

[0020] The air outlet ends of the first electronic control valve and the second electronic control valve are connected to the interior of the first box body 1 through the air inlet pipe;

[0021] A controller, the nitrogen generator and the gas heating chamber are both electrically connected to the controller;

[0022] The controller, the first electronic control valve and the second electronic control valve are electrically connected to the control host respectively.

[0023] Furthermore, a plurality of gas nozzles are connected to the side wall of the first box, including:

[0024] a plurality of first gas nozzles provided in the top area, wherein the first gas nozzles are connected to the first electronic control valve via an air inlet pipe;

[0025] A plurality of second gas nozzles are arranged in the bottom area, and the second gas nozzles are connected to the second electronic control valve through an air inlet pipe.

[0026] Furthermore, the jetting direction of the first gas nozzle is downward, forming an angle of 10°-30° with the horizontal downward direction;

[0027] The jetting direction of the second gas nozzle is upward, forming an angle of 45°-60° with the horizontal upward direction.

[0028] Furthermore, the vibration component includes:

[0029] a vibration base plate, fixed to the bottom of the second box;

[0030] A bottom support, fixed to the bottom of the vibration base plate;

[0031] The vibration bracket comprises two sets of brackets symmetrically arranged in the width direction of the base;

[0032] The spring is vertically connected between the two sets of vibration brackets;

[0033] The vibration motor is fixed in the middle of the vibration bracket and is electrically connected to the control host.

[0034] Furthermore, the exhaust assembly includes:

[0035] a vacuum pump connected to the exhaust hole of the second box through an exhaust pipe;

[0036] The vacuum pump is electrically connected to the control host.

[0037] Furthermore, an exhaust hole with a valve is provided at the bottom of the second box.

[0038] Beneficial effects of the above technical solution:

[0039] The present invention introduces heated protective gas into the first housing through a gas temperature-controlled purge assembly to directionally purge the solid fermentation material, thereby directly destroying the adhesion between the spores and the matrix (such as bran / rice husk), and weakening the moisture strengthening effect through drying, thereby solving the problem of spore residue and improving the yield;

[0040] At the same time, the exhaust component establishes a negative pressure environment in the second box, coordinated with the micro-oscillation of the vibration component, and the coordinated material vibration effect of the ultrasonic action, forcing the fine and light spores to efficiently penetrate the multi-stage sieve plates and fall into the bags under the dual effects of gravity and negative pressure adsorption, completely suppressing upward drift, shortening the screening time, and reducing losses; the control host integrates and regulates the gas flow rate, negative pressure intensity and vibration parameters, optimizes the entire spore separation process, and significantly improves production economy and reliability. BRIEF DESCRIPTION OF THE DRAWINGS

[0041] In order to more clearly illustrate the technical solutions in the embodiments of the present application, the following briefly introduces the drawings required for use in the description of the embodiments. Obviously, the drawings described below are only some embodiments of the present application. For those skilled in the art, other drawings can be obtained based on these drawings without creative work.

[0042] Figure 1 This is a structural diagram of a spore screening and collection device after solid fermentation of a biopesticide provided in one embodiment of the present application.

[0043] Among them, 1. First box body; 2. Sealing top cover; 3. Fixing screws; 4. Exhaust assembly; 401. Vacuum pump; 402. Exhaust pipe; 501. Nitrogen generator; 502. Gas heating chamber; 503. First electronic control valve; 504. Controller; 505. First gas nozzle; 506. Second gas nozzle; 507. Electric heating element; 508. Second electronic control valve; 601. Vibrating bottom plate; 602. Bottom support; 603. Vibrating bracket; 604. Spring; 605. Vibrating motor; 7. Control host; 8. Valve; 9. Second box body; 10. Sieve plate; 11. Cloth bag; 12. Ultrasonic generator probe. DETAILED DESCRIPTION

[0044] The technical solutions in the application embodiments will be described clearly and completely below in conjunction with the drawings in the application embodiments. Obviously, the described embodiments are only part of the application embodiments, not all of the embodiments.

[0045] Examples of the embodiments are shown in the accompanying drawings, wherein the same or similar symbols throughout represent the same or similar elements or elements having the same or similar functions. The embodiments described below with reference to the accompanying drawings are exemplary and are intended to be used to explain the application and are not to be construed as limiting the application.

[0046] In a specific embodiment of the present application, a biopesticide solid fermentation spore screening and collection device is provided, comprising: a first box body 1, the top of which is provided with a sealed top cover 2 and is sealed by fixing screws 3, and a multi-stage sieve plate 10 is provided inside; a gas temperature-controlled purge assembly is connected to the first box body 1 and continuously passes heated gas into the box, and the adhesion between the spores and the solid matrix is ​​destroyed by directional airflow purge; a second box body 9 is connected to the bottom of the first box body 1, and the inside is provided with a detachable cloth bag 11 and the side wall is connected to the exhaust assembly 4. With the help of the negative pressure environment established by the exhaust assembly 4 and the mechanical oscillation of the vibration assembly, the detached spores can efficiently penetrate the sieve plate 10 and be adsorbed on the cloth bag 11, thereby completely eliminating the spore escape loss; the vibration assembly is installed at the bottom of the second box body 9 to provide oscillation assistance; a control host 7 is electrically connected to the gas temperature-controlled purge assembly, the exhaust assembly 4 and the vibration assembly respectively; by uniformly controlling the gas flow rate, negative pressure intensity and vibration parameters, the screening requirements of different materials are accurately matched, and the process stability and repeatability are significantly improved. The device overcomes the dual problems of spore residue and dispersion in traditional screening through the integrated design of multi-stage screening, protective gas fluidization, negative pressure collection and intelligent control.

[0047] In the embodiments of this application, the core design of using nitrogen as the gas medium is based on the physiological characteristics of living microbial spores: when microbial pesticide spores are heated in an oxygen-containing air environment, they trigger intense respiratory metabolism, resulting in damage to the cell membrane structure and loss of activity. The nitrogen environment significantly inhibits these aerobic respiratory reactions by efficiently replacing oxygen within the device, fundamentally avoiding heat-induced inactivation of living spores. At the same time, the directional dry airflow formed by the heated nitrogen synergistically acts on the spore-matrix separation process during the purge process, maintaining the integrity of the spore biological activity while improving separation efficiency through a physical stripping mechanism.

[0048] In a specific embodiment of the present application, an openable and closable sealed door structure is integrated on the side wall of the second box body 9. The door body is quickly opened and closed through a hinge connection and a snap locking mechanism, forming an operating channel that directly leads to the collection bag 11, thereby solving the problem of removing materials from a closed container while maintaining the airtightness of the system.

[0049] In a specific embodiment of the present application, the multi-stage sieve plate 10 arranged inside the first box 1 breaks through the bottleneck of traditional technology through a dual design: first, a gradient pore size distribution system that decreases step by step from top to bottom is constructed (the example in the figure is a three-layer structure), forming a hierarchical filtration mechanism - the upper layer intercepts solid matrix residues, the middle layer blocks mycelium fragments, and the bottom layer accurately captures target spores; secondly, a dynamic expandable architecture is adopted, so that the number of sieve plates 10 can be flexibly increased according to the physical properties of different fungal spores (such as expanding the level for ultra-fine spores of Beauveria bassiana), realizing stepless adaptation from basic separation to high-precision interception.

[0050] In a specific embodiment of the present application, an ultrasonic generator probe 12 is built into the upper chamber of the first-stage sieve plate 10 of the first housing 1. This probe is directly connected to the control host 7 via a wire, enabling intelligent control of ultrasonic high-frequency vibration. Its operating principle is to directly break down the physical adhesion between spores and the solid matrix through the high-frequency cavitation effect, and in conjunction with the nitrogen purge of the gas temperature-controlled purge assembly, significantly accelerate the spore detachment process. This design has significant beneficial effects: ultrasonic pre-loosening significantly shortens the screening time to within 15 minutes and effectively eliminates spore residue under the microscope; closed-type non-contact conduction reduces energy consumption and avoids cross-contamination; and integration with the control host 7 enables parameter adaptive matching, improving the device's universality for a variety of fungal spores, such as Paecilomyces lilacinus and Beauveria bassiana.

[0051] In a specific embodiment of the present application, a gas temperature-controlled purge assembly includes: a nitrogen generator 501 with its outlet connected to a gas heating chamber 502, which houses an integrated electric heating element 507 (electric heating wire) and a temperature sensor; a dual-path pipeline at the outlet of the heating chamber, connected to the inlet of a first electronically controlled valve 503 and a second electronically controlled valve 508, respectively; the outlets of both valves communicate with the interior of the first housing 1 via independent pressure-resistant pipelines; a controller 504 electrically connected to the nitrogen generator 501, the gas heating chamber 502, and the dual-control valves, respectively, and establishes a bidirectional communication link with a control host 7. The temperature sensor monitors the airflow temperature in real time and provides feedback to the control host 7, which adjusts the power of the electric heating element 507 via the controller 504 to maintain a constant temperature of 40°C. This design synergistically innovates the spore separation process: directional purge of dry nitrogen simultaneously disrupts spore-matrix adhesion and moisture-enhancing effects; the dual-control valves intelligently switch purge timing to optimize airflow distribution; and the fully enclosed inert environment blocks oxygen contact, eliminating both spore activity loss and environmental pollution risks.

[0052] In a specific embodiment of the present application, the side wall of the first box body 1 adopts a partitioned gas nozzle architecture: a plurality of first gas nozzles 505 are arranged in the top area, connected to the first electronic control valve 503 through an independent pipe, and the jet direction is downward and at a precise angle of 10°-30° with the horizontal downward, forming a directional impact flow on the surface spore adhesion surface; a plurality of second gas nozzles 506 are arranged in the bottom area, connected to the second electronic control valve 508 through an independent pipe, and the jet direction is upward and at an optimized angle of 45°-60° with the horizontal upward, driving the bottom substrate to dynamically roll.

[0053] This design uses the spatial coordination of downward-angle stripping (10°-30°) and upward-angle fluidization (45°-60°) to construct a dual action chain of "breaking surface adhesion to removing bottom-layer dead corners": the downward airflow directly impacts the spore-matrix binding interface, breaking up biological adhesion; the upward airflow promotes the movement of material layers, eliminating screening blind spots; and the independent pipeline air supply avoids airflow interference and maximizes nitrogen utilization.

[0054] In a specific embodiment of the present application, the vibration assembly includes a vibration base plate 601, which is rigidly connected to the bottom of the second box body 9, and a bottom support 602 is fixed to the bottom of the vibration base plate 601 to form a bearing base; two groups of symmetrical vibration brackets 603 are arranged horizontally along the width direction of the bottom support 602, and springs 604 are vertically connected to the bracket groups to form a buffer interface; the vibration motor 605 is fixed in the center of the vibration bracket 603 and is directly connected to the control host 7 through a wire to receive parameter control. When in use, the control host 7 sends a command to activate the rotor system of the vibration motor 605, generating centrifugal force to drive the symmetrically distributed vibration brackets 603 to oscillate horizontally, eliminating eccentric torque; the spring 604 is vertically connected to the vibration bracket 603 groups to form a buffer interface, filtering high-frequency harmonics and converting them into micro-oscillations strictly perpendicular to the screen plate 10; the vertical vibration is transmitted to the second box body 9 through the rigidly connected vibration base plate 601, driving the material layer to loosen. This design eliminates eccentric torque through symmetrical bracket layout to ensure stable operation of the equipment under high-frequency oscillation; the central configuration of the motor combined with intelligent adjustment enables the vibration frequency to form a temporal and spatial coordination with gas purge and negative pressure collection, significantly improving the spore penetration efficiency.

[0055] In a specific embodiment of the present application, the exhaust assembly 4 includes a vacuum pump 401, which is directly connected to the exhaust hole on the side wall of the second box 9 via an exhaust pipe 402 and is electrically connected to the control host 7 to achieve intelligent regulation of the negative pressure intensity. At the same time, an exhaust hole with a valve 8 is provided at the bottom of the second box 9 for pressure balance and safe exhaust. This design uses the vacuum pump 401 to generate a negative pressure environment, efficiently sucking spores through the sieve plate 10 and adsorbing them to the bag 11, completely eliminating spore loss due to drift. The exhaust hole controlled by the valve 8 dynamically adjusts the pressure within the system to avoid equipment loss caused by overpressure. The integrated management of the control host 7 ensures that the negative pressure parameters are precisely matched with the vibration and airflow operations, significantly improving the spore yield and ensuring biosafety.

[0056] When in use, the general operating process is as follows:

[0057] Stage 1: Pretreatment and Spore Decontamination: After the biopesticide solid fermentation material is added to the first chamber 1, the chamber is sealed with the top cover 2 and screws 3. The ultrasonic generator probe 12 is turned on, and the gas temperature-controlled purge assembly is activated through the control host 7. Nitrogen is generated by the nitrogen generator 501 and maintained at a constant temperature of 40°C in the gas heating chamber 502. The first and second electronic control valves 503 and 508 adjust the flow rate to 0.5-50 L / min, and a cyclic purge sequence is executed. First, the top first electronic control valve 503 is opened to open the first gas nozzle 505, while the second electronic control valve 508 remains closed. Then, the bottom second electronic control valve 508 is opened to open the second gas nozzle 506, while the first electronic control valve 503 remains closed. This stage completely separates the spores from the solid matrix through the synergistic effect of ultrasonic cavitation and directional hot nitrogen fluidization.

[0058] Phase 2: Negative Pressure and Vibration Collection: Turn off the nitrogen purge and keep the ultrasonic probe active. Activate vacuum pump 401 of exhaust assembly 4 to create a negative pressure environment within second chamber 9. Simultaneously activate the vibration assembly to drive spores through the multi-stage sieve plate 10. The spores efficiently fall into the bag 11 under the combined effects of negative pressure and mechanical vibration, completing the bulk collection.

[0059] Stage 3: Residual Removal and System Reset: To eliminate residual spores, restart nitrogen generator 501, setting the gas temperature to 40°C and the flow rate to 0.1-1 L / min. Only the first top gas nozzle is activated for directional purge. The ultrasonic, vacuum pump 401, and vibration components are maintained in operation, allowing residual spores to penetrate sieve plate 10 and enter bag 11. Finally, close all components, open the vent at the bottom of second chamber 9 to relieve pressure, and remove bag 11 to obtain pure spores.

[0060] In an extended embodiment, this scheme is applicable to the screening and collection of Paecilomyces lilacinus spores, and the process is as follows:

[0061] Phase 1: Ultrasound-airflow synergistic peeling

[0062] Add 10 kg of crushed solid fermentation product of Paecilomyces lilacinus to the first chamber 1 and seal the chamber with the sealed top cover 2 and fixing screws 3. Turn on the ultrasonic generator probe 12 and activate the gas temperature-controlled purge assembly through the control host 7: turn on the nitrogen generator 501 and set the temperature of the gas heating chamber 502 to 40°C. Adjust the nitrogen flow rate to 10.0 L / min with the first electronic control valve 503 and the second electronic control valve 508. Execute the cyclic purge procedure:

[0063] 1. Top purge: Open the first electronic control valve 503 and operate the top first gas nozzle 505 for 15 seconds (the second electronic control valve 508 is closed at this time).

[0064] 2. Bottom purge: close the first electronic control valve 503, open the second electronic control valve 508, and operate the second gas nozzle 506 at the bottom for 15 seconds.

[0065] Repeat the above steps (step 1 and step 2) for 15 minutes. Repeated purging with dry nitrogen and ultrasonication will dry the matrix and completely separate the spores from the solid fermentation matrix.

[0066] Phase 2: Negative pressure vibration efficient collection

[0067] Turn off the nitrogen purge and keep the ultrasonic generator probe 12 on. Start the vacuum pump 401 to create a negative pressure environment, and simultaneously start the vibration motor 605. Under the dual action of vacuum adsorption and vertical vibration, the Paecilomyces lilacinus spores penetrate the multi-stage sieve plate 10 and efficiently fall into the collection bag 11. To remove any remaining spores, restart the nitrogen generator 501 to maintain a constant temperature of 40°C. The first electronic control valve 503 adjusts the flow rate to 1 L / min, and only the first gas nozzle 505 is turned on for directional purge. Keep the ultrasonic, vacuum pump, and vibration components running until the spores are completely collected.

[0068] Phase 3: Result Verification and Output

[0069] After the device was turned off, the collection bag 11 was removed to obtain 520 g of light purple fine spore powder. Microscopic observation showed that no spores remained in the solid fermentation product, and the spores of Paecilomyces purpurogenum were light purple fine powder.

[0070] In an extended embodiment, this scheme is applicable to the screening and collection of Trichoderma harzianum spores, and the process is as follows:

[0071] Phase 1: Material loading and ultrasonic-gas synergistic pretreatment

[0072] 10 kg of crushed solid fermentation product of Trichoderma harzianum was added to the first box 1, covered with a sealed top cover 2, and the box was sealed with fixing screws 3. Subsequently, the ultrasonic generator probe 12 was turned on, and the gas temperature control purge component was started through the control host 7: the nitrogen generator 501 was started, the temperature of the gas heating chamber 502 was set to 40°C, and the nitrogen flow rate was adjusted to 15.0 L / min through the first electronic control valve 503 and the second electronic control valve 508. The cyclic purge program was executed:

[0073] 1. Start the top circulation: open the first electronic control valve 503 and run the top first gas nozzle 505 for 60 seconds, while keeping the second electronic control valve 508 closed.

[0074] 2. Start the bottom circulation: close the first electronic control valve 503, open the second electronic control valve 508, and run the second gas nozzle 506 at the bottom for 60 seconds.

[0075] Repeat the above steps 1 and 2 for 15 minutes. During this process, the dry nitrogen purge and ultrasonic high-frequency vibration work together to completely separate the Trichoderma harzianum spores from the solid matrix and gradually dry the matrix.

[0076] Phase 2: Negative pressure vibration main collection

[0077] Turn off the nitrogen purge and keep the ultrasonic probe on. Start vacuum pump 401 of exhaust assembly 4 to create a negative pressure environment within second housing 9. Simultaneously activate the vibration assembly to drive the spores through the multi-stage sieve plate 10. The spores efficiently fall into the bag 11 through the synergistic effect of negative pressure adsorption and mechanical vibration.

[0078] Phase 3: Residue Optimization and Result Verification

[0079] To remove any remaining spores, the nitrogen generator 501 was restarted. The gas heating chamber 502 was maintained at 40°C, and the first electronically controlled valve 503 was adjusted to a flow rate of 5 L / min. Only the top first electronically controlled valve 503 was opened to allow the first gas nozzle 505 to perform a directional purge. The ultrasonic probe, vacuum pump 401, and vibration assembly were kept running to allow any remaining spores to penetrate the sieve plate 10 and enter the bag 11. After closing all components, the bag 11 was removed, yielding 1050 g of Trichoderma harzianum spores. Microscopic observation of the solid fermentation product revealed no remaining spores, and the spores appeared as a dark green dry powder.

[0080] In an extended embodiment, this solution is applicable to the screening and collection of Beauveria bassiana spores, and the process is as follows:

[0081] Phase 1: Ultrasound-airflow synergistic peeling

[0082] 10 kg of crushed Beauveria bassiana solid fermentation product was added to the first housing 1, which was then sealed with a sealed top cover 2 and fixing screws 3. Subsequently, the ultrasonic generator probe 12 was turned on, and the gas temperature-controlled purge assembly was activated via the control host 7. The nitrogen generator 501 was activated, the temperature of the gas heating chamber 502 was set to 40°C, and the nitrogen flow rate was adjusted to 7.0 L / min via the first and second electronic control valves 503 and 508.

[0083] To perform a cyclic purge procedure:

[0084] 1. Top purge: Open the first electronic control valve 503 and run the first gas nozzle 505 on the top for 30 seconds (the second electronic control valve (508) is closed at this time).

[0085] 2. Bottom purge: close the first electronic control valve 503, open the second electronic control valve 508, and operate the second gas nozzle 506 at the bottom for 30 seconds.

[0086] Repeat the above steps (step 1 and step 2) for 15 minutes. During this process, the dry nitrogen purge and ultrasonic high-frequency vibration work together to break the spore adhesion and completely separate the Beauveria bassiana spores from the matrix.

[0087] Phase 2: Negative pressure vibration efficient collection

[0088] Turn off the nitrogen purge and keep ultrasonic generator probe 12 on. Start vacuum pump 401 to create a negative pressure environment in second chamber 9, and simultaneously activate the vibration assembly. Under the dual effects of negative pressure adsorption and mechanical vibration, spores penetrate the multi-stage sieve plate 10 and efficiently fall into the bag 11.

[0089] Phase 3: Directed Optimization and Result Verification

[0090] Restart nitrogen generator 501, maintaining a constant temperature of 40°C. Adjust the flow rate to 0.7 L / min with first electronic control valve 503, and activate only first gas nozzle 505 for directional purge. Maintain ultrasound, vacuum pump 401, and vibration operation to remove any remaining spores into bag 11. After shutting down the equipment and removing bag 11, 760 g of off-white spore powder was obtained. Microscopic examination revealed no spores remaining on the solid matrix.

[0091] Throughout this specification, references to terms such as "one embodiment," "some embodiments," "example," "specific example," "a specific embodiment," or "some examples" mean that the specific features, structures, materials, or characteristics described in conjunction with that embodiment or example are included in at least one embodiment or example of the application. In this specification, schematic representations of terms do not necessarily refer to the same embodiment or example. Furthermore, the specific features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

[0092] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, rather than to limit them. Although the present application has been described in detail with reference to the aforementioned embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the aforementioned embodiments, or make equivalent replacements for some of the technical features therein. However, these modifications or replacements do not cause the essence of the corresponding technical solutions to deviate from the spirit and scope of the technical solutions of the embodiments of the present application.

Claims

1. A spore screening and collection device after solid fermentation of biological pesticides, characterized in that: include: The first box (1) is provided with a sealing top cover (2) on the top and a multi-stage sieve plate (10) is arranged inside the first box; a gas temperature-controlled purge component, connected to the first box (1), for introducing heated gas into the box to purge the solid fermentation material; A second box (9) is connected to the bottom of the first box (1), is provided with a cloth bag (11) inside, and its side wall is connected to the exhaust assembly (4); A vibration component is installed at the bottom of the second box (9); The control host (7) is electrically connected to the gas temperature control purge component, the exhaust component (4) and the vibration component respectively.

2. The spore screening and collection device after solid fermentation of biopesticide according to claim 1, characterized in that: An ultrasonic generator probe (12) is provided in the first box (1), the probe being located in the upper chamber of the first-stage sieve plate (10) and electrically connected to the control host (7).

3. The spore screening and collection device after solid fermentation of biopesticide according to claim 1, characterized in that: The sieve hole diameters of the multi-stage sieve plates (10) decrease step by step from top to bottom.

4. The spore screening and collection device after solid fermentation of biopesticide according to claim 1, characterized in that: The gas temperature control purge assembly includes: A nitrogen generator (501), the gas outlet of which is connected to the gas heating chamber (502); A gas heating chamber (502) is provided with an electric heating element (507) therein, and a gas outlet of the gas heating chamber (502) is connected to the gas inlet of a first electronic control valve (503) and the gas inlet of a second electronic control valve (508) in two ways through an air inlet pipe; The gas outlet ends of the first electronic control valve (503) and the second electronic control valve (508) are connected to the interior of the first box (1) through the gas inlet pipe; A controller (504), wherein the nitrogen generator (501) and the gas heating chamber (502) are both electrically connected to the controller (504); The controller (504), the first electronic control valve (503) and the second electronic control valve (508) are all electrically connected to the control host (7).

5. The spore screening and collection device after solid fermentation of biopesticide according to claim 4, characterized in that: A plurality of gas nozzles are connected to the side wall of the first box (1), including: a plurality of first gas nozzles (505) provided in the top region, wherein the first gas nozzles (505) are connected to the first electronic control valve (503) via an air inlet pipe; A plurality of second gas nozzles (506) are arranged in the bottom area, and the second gas nozzles (506) are connected to the second electronic control valve (508) through an air inlet pipe.

6. The spore screening and collection device after solid fermentation of biopesticide according to claim 5, characterized in that: The jetting direction of the first gas nozzle (505) is downward, forming an angle of 10°-30° with the horizontal downward direction; The jetting direction of the second gas nozzle (506) is upward, forming an angle of 45°-60° with the horizontal upward direction.

7. The spore screening and collection device after solid fermentation of a biopesticide according to any one of claims 1 to 6, characterized in that: The vibration assembly comprises: A vibration base plate (601) is fixed to the bottom of the second box (9); A bottom support (602) is fixed to the bottom of the vibration base plate (601); The vibration bracket (603) comprises two groups of brackets symmetrically arranged in the width direction of the base (602); A spring (604) is vertically connected between the two sets of vibration brackets (603); The vibration motor (605) is fixed to the middle of the vibration bracket (603) and is electrically connected to the control host (7).

8. The spore screening and collection device after solid fermentation of a biopesticide according to any one of claims 1 to 6, characterized in that: The exhaust assembly (4) comprises: A vacuum pump (401) is connected to the air extraction hole of the second box (9) via an air extraction pipe (402); The vacuum pump (401) is electrically connected to the control host (7).

9. The spore screening and collection device after solid fermentation of a biopesticide according to any one of claims 1 to 6, characterized in that: The bottom of the second box (9) is provided with an exhaust hole with a valve (8).

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