Stem cell culture device

Abstract

The invention provides a stem cell culture device, and belongs to the technical field of stem cell culture, the stem cell culture device comprises a culture box and further comprises an opening and closing structure, the opening and closing structure is arranged at the top of the culture box and is used for sealing the culture box; the lifting plate is arranged in the incubator in a sliding manner. According to the stem cell culture device disclosed by the invention, by arranging the rotary shaking structure and the heating plate, the culture tube filled with stem cells can rotate while revolving, and the culture tube filled with the stem cells can be shaken in the rotation process, so that the stem cells in the culture tube are in uniform contact with nutrient substances and oxygen; therefore, the growth and reproduction of the stem cells are promoted, the culture efficiency is improved, a plurality of culture tubes can be uniformly heated, the phenomenon of non-uniform heating of the stem cells is avoided, the culture effect of the stem cells is improved, and the problems of low efficiency and non-uniform heating of the stem cells caused by static culture in the prior art are solved.

Description

Technical Field

[0001] The present invention relates to the technical field of stem cell culture, and in particular to a stem cell culture device. Background Art

[0002] Stem cells are multipotent cells with the ability to self-replicate. Under certain conditions, they can differentiate into a variety of functional cells. Depending on their developmental stage, they are categorized as embryonic stem cells and adult stem cells. Stem cells are immature, underdifferentiated cells with the potential to regenerate various tissues, organs, and the human body. They are known in the medical community as "universal cells." Therefore, the cultivation of stem cells is extremely important.

[0003] Currently, there are the following problems in culturing stem cells:

[0004] (1) Existing technologies usually culture stem cells statically, which prevents the stem cells from evenly contacting nutrients and oxygen, resulting in slow growth and reproduction of stem cells and low stem cell culture efficiency. At the same time, the heating source during culture cannot be evenly covered, and static culture causes uneven heating of the stem cells, reducing the culture effect;

[0005] (2) In the prior art, when culturing stem cells, the lid of the incubator usually needs to be opened and closed manually, which has a low degree of automation and is inconvenient for placing the culture tubes.

[0006] Therefore, we made improvements to this and proposed a stem cell culture device. Summary of the Invention

[0007] The purpose of the present invention is to address the problems of currently existing static stem cell culture, which leads to low culture efficiency, inconvenience in uniform heating of stem cells, inconvenience in automatic lid opening and inconvenience in placing culture tubes.

[0008] In order to achieve the above-mentioned object of the invention, the present invention provides the following technical solutions:

[0009] A stem cell culture device is provided to improve the above problems.

[0010] The present invention is specifically as follows:

[0011] Including incubator, also includes,

[0012] An opening and closing structure, which is provided at the top of the incubator and is used to seal the incubator;

[0013] A lifting plate, the lifting plate being slidably disposed inside the incubator;

[0014] A lifting structure, the lifting structure is provided on the lifting plate and is used to adjust the position of the lifting plate;

[0015] The rotating and shaking structure includes a bracket detachably connected to the lifting plate by bolts, the upper end surface of the bracket is fixed with a first bearing, the inner side wall of the inner ring of the first bearing is fixedly connected with a rotating tube, the bottom end of the rotating tube passes through the bracket and is fixedly connected to the driven gear, the upper end surface of the bracket is fixedly connected to the power motor, the driving end of the power motor is fixedly connected to the driving gear meshing with the driven gear, the top end of the rotating tube is fixedly connected to the connecting frame, the top end of the connecting frame is fixed with a mounting ring, a vertical shaft is provided inside the rotating tube, and the bottom end of the vertical shaft is aligned with the bracket The bottom is fixedly connected, and the top of the vertical shaft is fixedly connected to the gear ring through a support rod. The upper end surface of the mounting ring is evenly fixedly connected to a group of second bearings, and the inner side wall of the inner ring of the second bearing is fixedly connected to a connecting tube. The bottom end of the connecting tube passes through the mounting ring and is fixedly connected to a follower gear meshing with the gear ring. The connecting tube is fixedly connected to a vertical plate, and the top of the vertical plate is rotatably connected to a reciprocating shaft, and the reciprocating shaft is fixedly connected to a vertical rod, and the vertical rod is fixedly connected to a carrier frame, a culture tube is provided in the carrier frame, and a shaking component for shaking the culture tube is provided on the vertical plate.

[0016] As a preferred technical solution of the present invention, the shaking assembly includes a fixed shaft arranged in a connecting tube, the bottom end of the fixed shaft is fixedly connected to a fixed seat, the fixed seat is fixedly connected to the mounting ring by a hexagon socket bolt, the top end of the fixed shaft is fixedly connected to a first bevel gear, the vertical plate is rotatably connected to a transmission shaft, the inner end of the transmission shaft is fixedly connected to a second bevel gear meshing with the first bevel gear, the outer end of the transmission shaft is fixedly connected to a rotating plate, the top end of the rotating plate is slidably connected to a sliding head, and the sliding head is slidably connected to the vertical rod.

[0017] As a preferred technical solution of the present invention, the opening and closing structure includes a box cover arranged on the upper side of the incubator, an assembly seat is fixedly connected to the rear side wall of the incubator, a connecting shaft is rotatably connected in the assembly seat, a connecting rod is fixedly connected to the connecting shaft, the inner end of the connecting rod is fixedly connected to the box cover, and the outer end of the connecting rod is rotatably connected in the inner end to a movable head, a telescopic cylinder is provided on the rear side of the incubator, the driving end of the telescopic cylinder is fixedly connected to the movable head, the bottom end of the telescopic cylinder is movably connected to a movable seat, and the movable seat is fixedly connected to the incubator.

[0018] As a preferred technical solution of the present invention, a circular installation opening is provided on the box cover, and a circular transparent plate is sealed and fixedly connected to the circular installation opening, and the straight line of the circular transparent plate is slightly smaller than the width of the lifting plate.

[0019] As a preferred technical solution of the present invention, the lifting structure includes an assembly frame arranged on the lower side of the lifting plate, the bottom end of the assembly frame is fixedly connected to the inner bottom wall of the incubator, the assembly frame is symmetrically and rotatably connected to two threaded rods, the two threaded rods are respectively threadedly connected to the lifting plate, the top ends of the two threaded rods are rotatably connected to a limit block, the limit block is fixedly connected to the incubator, the bottom ends of the two threaded rods pass through the assembly frame and are fixedly connected to a driven bevel gear, the assembly frame is rotatably connected with a connecting rod, the connecting rod is fixedly connected to two driving bevel gears that are respectively meshed with the driven bevel gear, the connecting rod is fixedly connected to a worm gear, the assembly frame is fixedly connected to an assembly frame, the assembly frame is fixedly connected to a servo motor, and the driving end of the servo motor passes through the assembly frame and is fixedly connected to a worm that is meshed with the worm gear.

[0020] As a preferred technical solution of the present invention, a heating plate is fixedly connected to the left inner wall of the incubator, and a group of ultraviolet germicidal lamps are evenly fixedly connected to the right inner wall of the incubator.

[0021] As a preferred technical solution of the present invention, a temperature sensor is fixedly connected to the rear inner wall of the incubator, and a controller is connected to the front wall of the incubator, and the controller is electrically connected to the temperature sensor.

[0022] As a preferred technical solution of the present invention, a rectangular opening is provided on the front side wall of the incubator, and a rectangular transparent plate is sealed and fixedly connected in the rectangular opening.

[0023] As a preferred technical solution of the present invention, handles are fixedly connected to the left and right side walls of the incubator, and rubber sleeves are fixedly provided on the handles.

[0024] As a preferred technical solution of the present invention, a non-slip pad is fixedly connected to the bottom of the incubator, and the bottom surface of the non-slip pad is provided with non-slip grooves.

[0025] Compared with the prior art, the present invention has the following beneficial effects:

[0026] In the solution of the present invention:

[0027] 1. The rotating and shaking structure and heating plate enable the culture tubes containing stem cells to rotate while revolving. The rotation also causes the culture tubes containing stem cells to shake, allowing the stem cells in the culture tubes to be evenly exposed to nutrients and oxygen, thereby promoting the growth and reproduction of stem cells and improving culture efficiency. The system also allows multiple culture tubes to be evenly heated, avoiding uneven heating of stem cells and improving the culture effect of stem cells. This solves the problems of low efficiency and uneven heating of stem cells caused by static culture in the prior art.

[0028] 2. Through the incubator, opening and closing structure and lifting structure, the lid of the incubator can be opened and closed automatically, without the need for operators to manually open and close the lid. The operation is simple and the degree of automation is high. The height of the rotating and shaking structure can also be automatically adjusted to facilitate the placement and removal of culture tubes, solving the problem of cumbersome manual opening of the lid and inconvenience in placing culture tubes in the prior art. BRIEF DESCRIPTION OF THE DRAWINGS

[0029] Figure 1 A schematic diagram of the expanded structure provided by the present invention;

[0030] Figure 2 A schematic diagram of the internal structure of the incubator provided by the present invention;

[0031] Figure 3 A schematic diagram of the lifting structure provided by the present invention;

[0032] Figure 4 A schematic diagram of the bottom structure of the rotating and shaking structure provided by the present invention;

[0033] Figure 5 A schematic diagram of the upper structure of the rotating and shaking structure provided by the present invention;

[0034] Figure 6 The present invention provides Figure 4 A schematic diagram of the front structure of FIG.

[0035] Figure 7 A schematic diagram of a portion of the structure of the rotating and shaking structure provided by the present invention;

[0036] Figure 8 A schematic diagram of the structure of the shaking assembly provided by the present invention;

[0037] Figure 9 A schematic diagram of the opening and closing structure provided by the present invention;

[0038] Figure 10 A schematic diagram of the closed structure provided by the present invention;

[0039] Figure 11 The present invention provides Figure 1 Schematic diagram of the top view structure.

[0040] Indicated in the figure:

[0041] 1. Incubator; 2. Opening and closing structure; 201. Box cover; 202. Assembly seat; 203. Connecting shaft; 204. Connecting rod; 205. Movable head; 206. Telescopic cylinder; 207. Movable seat; 208. Circular transparent plate; 3. Lifting plate; 4. Lifting structure; 401. Assembly frame; 402. Threaded rod; 403. Limit block; 404. Driven bevel gear; 405. Connecting rod; 406. Driving bevel gear; 407. Worm gear; 408. Assembly frame; 409. Servo motor; 4010. Worm; 5. Rotating and shaking structure; 501. Bracket; 502. First bearing; 503. Rotating tube; 504. Driven gear; 505. Power motor; 506. Driving gear; 50 7. Connecting frame; 508. Mounting ring; 509. Vertical axis; 5010. Support rod; 5011. Ring gear; 5012. Second bearing; 5013. Connecting pipe; 5014. Follower gear; 5015. Vertical plate; 5016. Reciprocating shaft; 5017. Vertical rod; 5018. Carrying frame; 5019. Culture tube; 5020. Shaking assembly; 5021. Fixed axis; 5022. Fixed seat; 5023. First bevel gear; 5024. Transmission shaft; 5025. Second bevel gear; 5026. Rotating plate; 5027. Sliding head; 6. Heating plate; 7. Ultraviolet germicidal lamp; 8. Temperature sensor; 9. Controller; 10. Rectangular transparent plate; 11. Handle; 12. Anti-slip mat. DETAILED DESCRIPTION

[0042] To make the purpose, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them.

[0043] like Figures 1-11 As shown, this embodiment provides a stem cell culture device, including an incubator 1, and further including:

[0044] An opening and closing structure 2 is provided on the top of the incubator 1 and is used to seal the incubator 1;

[0045] A lifting plate 3 is slidably arranged inside the incubator 1;

[0046] A lifting structure 4 is provided on the lifting plate 3 and is used to adjust the position of the lifting plate 3;

[0047] The rotating and shaking structure 5 includes a bracket 501 on the lifting plate 3 which is detachably connected by bolts, a first bearing 502 being fixed on the upper end surface of the bracket 501, a rotating tube 503 being fixedly connected to the inner side wall of the inner ring of the first bearing 502, the bottom end of the rotating tube 503 passes through the bracket 501 and is fixedly connected to the driven gear 504, the upper end surface of the bracket 501 is fixedly connected to the power motor 505, the driving end of the power motor 505 is fixedly connected to the driving gear 506 which is meshed with the driven gear 504, the top of the rotating tube 503 is fixedly connected to the connecting frame 507, the top of the connecting frame 507 is fixed with the mounting ring 508, a vertical shaft 509 is provided inside the rotating tube 503, the bottom end of the vertical shaft 509 is fixedly connected to the bottom of the bracket 501, the top of the vertical shaft 509 is fixedly connected to the bottom of the bracket 501 The end is fixedly connected to the gear ring 5011 through the support rod 5010, and a group of second bearings 5012 are evenly fixedly connected to the upper end surface of the mounting ring 508. The inner side wall of the inner ring of the second bearing 5012 is fixedly connected to a connecting tube 5013. The bottom end of the connecting tube 5013 passes through the mounting ring 508 and is fixedly connected to a follower gear 5014 meshing with the gear ring 5011. The connecting tube 5013 is fixedly connected to a vertical plate 5015, and the top of the vertical plate 5015 is rotatably connected to a reciprocating shaft 5016. The reciprocating shaft 5016 is fixedly connected to a vertical rod 5017, and the vertical rod 5017 is fixedly connected to a carrier frame 5018. A culture tube 5019 is provided in the carrier frame 5018, and a shaking component 5020 for shaking the culture tube 5019 is provided on the vertical plate 5015.

[0048] The driving gear 506 is driven to rotate by the power motor 505. The rotation of the driving gear 506 drives the driven gear 504 and the rotating tube 503 to rotate. The rotation of the rotating tube 503 rotates the connecting frame 507 and the mounting ring 508. The rotation of the mounting ring 508 causes the stem cells to revolve. At the same time, the rotation of the mounting ring 508 causes the connecting tube 5013 and the follower gear 5014 to rotate simultaneously. The follower gear 5014 rotates around the ring gear 5011, thereby rotating the connecting tube 5013 and the vertical plate 5015. The rotation of the vertical plate 5015 causes the carrier frame 5018 to rotate. This allows the stem cells to rotate while revolving, so that the stem cells can be heated evenly, thereby improving the culture effect of the stem cells.

[0049] like Figure 3 、 Figure 4 、 Figure 5 、 Figure 6 、 Figure 7 and Figure 8As shown, as a preferred embodiment, on the basis of the above-mentioned method, further, the shaking assembly 5020 includes a fixed shaft 5021 arranged in the connecting tube 5013, the bottom end of the fixed shaft 5021 is fixedly connected to a fixed seat 5022, and the fixed seat 5022 is fixedly connected to the mounting ring 508 by an inner hexagonal bolt, and the top of the fixed shaft 5021 is fixedly connected to the first bevel gear 5023, and the vertical plate 5015 is rotatably connected to the transmission shaft 5024, the inner end of the transmission shaft 5024 is fixedly connected to the second bevel gear 5025 meshing with the first bevel gear 5023, and the outer end of the transmission shaft 5024 is fixedly connected to the rotating plate 5026, and the top of the rotating plate 5026 is slidably connected to the sliding head 5027, and the sliding head 5027 is connected to the vertical plate 5015. The rod 5017 is slidably connected; since the rotation of the connecting tube 5013 will drive the vertical plate 5015 to rotate, the rotation of the vertical plate 5015 drives the transmission shaft 5024 and the second bevel gear 5025 to rotate around the first bevel gear 5023, and at the same time, the first bevel gear 5023 on the fixed shaft 5021 is meshed and connected with the second bevel gear 5025 on the transmission shaft 5024, so that the transmission shaft 5024 rotates, and the rotation of the transmission shaft 5024 drives the rotary plate 5026 to rotate, and the sliding head 5027 on the rotary plate 5026 slides on the vertical rod 5017, thereby driving the reciprocating shaft 5016 and the carrier 5018 to shake back and forth, which is convenient for dynamic culture of stem cells and helps to evenly distribute and grow stem cells in the culture tube 5019.

[0050] like Figure 1 、 Figure 9 and Figure 10 As shown, as a preferred embodiment, on the basis of the above-mentioned manner, the opening and closing structure 2 further includes a box cover 201 arranged on the upper side of the incubator 1, a mounting base 202 is fixedly connected to the rear side wall of the incubator 1, a connecting shaft 203 is rotatably connected in the mounting base 202, a connecting rod 204 is fixedly connected to the connecting shaft 203, the inner end of the connecting rod 204 is fixedly connected to the box cover 201, and the outer end of the connecting rod 204 is rotatably connected to the movable head 205, and a telescopic cylinder 206 is provided on the rear side of the incubator 1. The driving end of 206 is fixedly connected to the movable head 205, and the bottom end of the telescopic cylinder 206 is movably connected to the movable seat 207, and the movable seat 207 is fixedly connected to the incubator 1; when the telescopic cylinder 206 is started, its driving end pushes the movable head 205 to move. Since the movable head 205 is rotatably connected to the outer end of the connecting rod 204, the inner end of the connecting rod 204 drives the box cover 201 to rotate around the connecting shaft 203, thereby realizing the opening or closing of the incubator 1, and the box cover 201 is automatically opened and closed, which is convenient to operate and has a high degree of automation.

[0051] like Figure 9 and Figure 10As shown, as a preferred embodiment, on the basis of the above method, a circular mounting opening is further provided on the box cover 201, and a circular transparent plate 208 is sealed and fixedly connected to the circular mounting opening, and the straight drop of the circular transparent plate 208 is slightly smaller than the width of the lifting plate 3; the setting of the circular transparent plate 208 allows the situation inside the incubator 1 to be observed without opening the box cover 201, thereby making it convenient to check the culture status of the stem cells.

[0052] like Figure 1 、 Figure 2 and Figure 3 As shown, as a preferred embodiment, on the basis of the above-mentioned manner, further, the lifting structure 4 includes an assembly frame 401 arranged on the lower side of the lifting plate 3, the bottom end of the assembly frame 401 is fixedly connected to the inner bottom wall of the incubator 1, and two threaded rods 402 are symmetrically and rotatably connected to the assembly frame 401, the two threaded rods 402 are respectively threadedly connected to the lifting plate 3, the top ends of the two threaded rods 402 are rotatably connected to the limit block 403, the limit block 403 is fixedly connected to the incubator 1, the bottom ends of the two threaded rods 402 pass through the assembly frame 401 and are fixedly connected to the driven bevel gear 404, the assembly frame 401 is rotatably connected with a connecting rod 405, and the connecting rod 405 is fixedly connected to two active bevel gears 406 that are respectively meshed with the driven bevel gear 404, and the connecting rod 405 is symmetrically and rotatably connected to the two threaded rods 402. A worm gear 407 is fixedly connected to the assembly frame 401, an assembly frame 408 is fixedly connected to the assembly frame 408, and a servo motor 409 is fixedly connected to the assembly frame 408. The driving end of the servo motor 409 passes through the assembly frame 408 and is fixedly connected to a worm 4010 meshing with the worm gear 407; after the servo motor 409 is started, its driving end drives the worm 4010 to rotate, and the meshing connection between the worm 4010 and the worm gear 407 causes the connecting rod 405 to rotate, thereby driving the two active bevel gears 406 to rotate, and the meshing connection between the active bevel gear 406 and the driven bevel gear 404 causes the two threaded rods 402 to rotate synchronously. Due to the rotation of the threaded rod 402, the lifting plate 3 is driven to move up and down, thereby adjusting its position, making it convenient to place and remove stem cells and avoiding the problem of inconvenient operation in a small space.

[0053] like Figure 1 、 Figure 2 and Figure 11 As shown, as a preferred embodiment, on the basis of the above method, a heating plate 6 is further fixedly connected to the left inner wall of the incubator 1, and a group of ultraviolet sterilization lamps 7 are evenly fixedly connected to the right inner wall of the incubator 1; the heating plate 6 provides a suitable growth temperature for stem cells, and the ultraviolet sterilization lamps 7 can regularly sterilize the inside of the incubator 1 to ensure the sterility of the culture environment.

[0054] like Figure 1As shown, as a preferred embodiment, on the basis of the above-mentioned method, further, a temperature sensor 8 is fixedly connected to the inner wall of the rear side of the incubator 1, and a controller 9 is connected to the front wall of the incubator 1, and the controller 9 is electrically connected to the temperature sensor 8; the temperature sensor 8 monitors the temperature in the incubator 1 in real time and transmits the data to the controller 9, and the controller 9 controls the heating plate 6 according to a preset temperature range to keep the temperature in the incubator 1 stable, thereby ensuring that the stem cells grow under optimal temperature conditions.

[0055] like Figure 1 and Figure 2 As shown, as a preferred embodiment, on the basis of the above-mentioned method, further, a rectangular opening is provided on the front side wall of the incubator 1, and a rectangular transparent plate 10 is sealed and fixedly connected in the rectangular opening; the provision of the rectangular transparent plate 10 allows the internal situation of the incubator 1 to be observed from the front without opening the incubator 1, thereby facilitating the observation of the culture status of the stem cells.

[0056] like Figure 1 and Figure 11 As shown, as a preferred embodiment, on the basis of the above-mentioned method, further, handles 11 are fixedly connected to the left and right side walls of the incubator 1, and rubber sleeves are fixedly covered on the handles 11; the setting of the handles 11 facilitates the transportation and movement of the incubator 1, and the rubber sleeves provide a comfortable grip and anti-slip effect, ensuring safety during operation.

[0057] like Figure 1 and Figure 9 As shown, as a preferred embodiment, on the basis of the above method, further, a non-slip pad 12 is fixedly connected to the bottom of the incubator 1, and the bottom surface of the non-slip pad 12 is provided with non-slip grooves; the provision of the non-slip pad 12 increases the stability of the incubator 1 when it is placed, prevents it from accidentally sliding, and ensures the safety and stability of the stem cell culture process.

[0058] Specifically, when the present stem cell culture device is in use: first, the driving end of the telescopic cylinder 206 pushes the movable head 205 to move, and the movement of the movable head 205 drives the connecting rod 204 to move, and the inner end of the connecting rod 204 drives the box cover 201 to rotate around the connecting shaft 203, thereby realizing the opening of the box cover 201. Then, the driving end of the servo motor 409 drives the worm 4010 to rotate, and the meshing connection between the worm 4010 and the worm wheel 407 causes the connecting rod 405 to rotate, thereby driving the two active bevel gears 406 to rotate, and the meshing connection between the active bevel gear 406 and the driven bevel gear 404 causes the two threaded rods 402 to rotate synchronously. The rotation of the threaded rod 402 drives the lifting plate 3 to rise, and then the culture tube 5019 containing stem cells is placed in the carrier 5018, and the servo motor 409 is driven to reverse to reset the lifting plate 3, and then the telescopic cylinder 206 is driven to close the box cover 201, and the ultraviolet sterilization lamp 7 is turned on to irradiate and sterilize the inside of the incubator 1. The temperature sensor 8 monitors the temperature in the incubator 1 in real time and transmits the data to the controller 9. The controller 9 controls the heating plate 6 according to the preset temperature range to maintain the temperature in the incubator 1 stable. At the same time, the power motor 505 is started, and the power motor 505 drives the driving gear 506 to rotate. The rotation of the driving gear 506 drives the driven gear 504 and the rotating tube 503 to rotate. The rotation of the rotating tube 503 rotates the connecting frame 507 and the mounting ring 508. The rotation of the mounting ring 508 causes the connecting tube 5013 and the driven gear 5014 to rotate simultaneously. The driven gear 5014 rotates around the gear ring 5011, thereby causing the connecting tube 5013 and the vertical plate 5015 to rotate. The rotation of the vertical plate 5015 causes the carrier frame 5018 to rotate. The rotation of the connecting tube 5013 drives the vertical plate 5015 to rotate. The rotation of the vertical plate 5015 drives the transmission shaft 5024 and the second bevel gear 5025 around It rotates around the first bevel gear 5023, and at the same time, the first bevel gear 5023 on the fixed shaft 5021 is meshed with the second bevel gear 5025 on the transmission shaft 5024, so that the transmission shaft 5024 rotates. The rotation of the transmission shaft 5024 drives the rotary plate 5026 to rotate, and the sliding head 5027 on the rotary plate 5026 slides on the vertical rod 5017, thereby driving the reciprocating shaft 5016 and the carrier frame 5018 to shake back and forth, so that the culture tube 5019 can rotate while it is revolving, and can also shake the stem cells while rotating, thereby promoting the growth and reproduction of stem cells and improving the culture efficiency.

[0059] All technical features in this embodiment can be freely combined according to actual needs.

[0060] The above embodiments are preferred implementation schemes of the present invention. In addition, the present invention can also be implemented in other ways. Any obvious replacement without departing from the concept of the present technical solution is within the scope of protection of the present invention.

Claims

1. A stem cell culture device, comprising an incubator (1), characterized in that: Also includes, an opening and closing structure (2), the opening and closing structure (2) being arranged on the top of the incubator (1) and being used to seal the incubator (1); A lifting plate (3), the lifting plate (3) being slidably arranged inside the incubator (1); A lifting structure (4), the lifting structure (4) being arranged on the lifting plate (3) and being used for adjusting the position of the lifting plate (3); The rotating and shaking structure (5) comprises a bracket (501) detachably connected to the lifting plate (3) by bolts, a first bearing (502) being fixed to the upper end surface of the bracket (501), a rotating tube (503) being fixedly connected to the inner side wall of the inner ring of the first bearing (502), the bottom end of the rotating tube (503) passing through the bracket (501) and being fixedly connected to the driven gear (504), and the upper end surface of the bracket (501) being fixedly connected to the moving gear (504). The power motor (505) is fixedly connected to the driving end of the power motor (505) with a driving gear (506) meshed with the driven gear (504). The top of the rotating tube (503) is fixedly connected to the connecting frame (507). The top of the connecting frame (507) is fixedly provided with a mounting ring (508). The interior of the rotating tube (503) is provided with a vertical shaft (509). The bottom end of the vertical shaft (509) is fixedly connected to the bottom of the bracket (501). The vertical shaft (509) is fixedly connected to the bottom of the bracket (501). 9) is fixedly connected to a gear ring (5011) through a support rod (5010), and a group of second bearings (5012) are evenly fixedly connected to the upper end surface of the mounting ring (508), and a connecting pipe (5013) is fixedly connected to the inner side wall of the inner ring of the second bearing (5012). The bottom end of the connecting pipe (5013) passes through the mounting ring (508) and is fixedly connected to a follower gear (5014) meshing with the gear ring (5011). The connecting pipe (501 3) is fixedly connected to a vertical plate (5015), the top end of the vertical plate (5015) is rotatably connected to a reciprocating shaft (5016), the reciprocating shaft (5016) is fixedly connected to a vertical rod (5017), the vertical rod (5017) is fixedly connected to a supporting frame (5018), a culture tube (5019) is provided in the supporting frame (5018), and a shaking component (5020) for shaking the culture tube (5019) is provided on the vertical plate (5015).

2. A stem cell culture device according to claim 1, characterized in that: The shaking assembly (5020) comprises a fixed shaft (5021) arranged in the connecting tube (5013), the bottom end of the fixed shaft (5021) is fixedly connected to a fixed seat (5022), the fixed seat (5022) is fixedly connected to the mounting ring (508) via a hexagon socket bolt, the top end of the fixed shaft (5021) is fixedly connected to a first bevel gear (5023), the vertical plate (5015) is rotatably connected to a transmission shaft (5024), the inner end of the transmission shaft (5024) is fixedly connected to a second bevel gear (5025) meshing with the first bevel gear (5023), the outer end of the transmission shaft (5024) is fixedly connected to a rotating plate (5026), the top end of the rotating plate (5026) is slidably connected to a sliding head (5027), and the sliding head (5027) is slidably connected to the vertical rod (5017).

3. The stem cell culture device according to claim 1, characterized in that: The opening and closing structure (2) comprises a box cover (201) arranged on the upper side of the incubator (1); an assembly seat (202) is fixedly connected to the rear side wall of the incubator (1); a connecting shaft (203) is rotatably connected inside the assembly seat (202); a connecting rod (204) is fixedly connected to the connecting shaft (203); an inner end of the connecting rod (204) is fixedly connected to the box cover (201); an outer end of the connecting rod (204) is rotatably connected to a movable head (205); a telescopic cylinder (206) is provided on the rear side of the incubator (1); a driving end of the telescopic cylinder (206) is fixedly connected to the movable head (205); a bottom end of the telescopic cylinder (206) is movably connected to a movable seat (207); and the movable seat (207) is fixedly connected to the incubator (1).

4. The stem cell culture device according to claim 3, characterized in that: A circular mounting opening is provided on the box cover (201), and a circular transparent plate (208) is sealed and fixedly connected in the circular mounting opening. The straight length of the circular transparent plate (208) is slightly smaller than the width of the lifting plate (3).

5. The stem cell culture device according to claim 1, characterized in that: The lifting structure (4) comprises an assembly frame (401) arranged on the lower side of the lifting plate (3), the bottom end of the assembly frame (401) is fixedly connected to the inner bottom wall of the incubator (1), and two threaded rods (402) are symmetrically and rotatably connected to the assembly frame (401), the two threaded rods (402) are respectively threadedly connected to the lifting plate (3), the top ends of the two threaded rods (402) are rotatably connected to the limit block (403), the limit block (403) is fixedly connected to the incubator (1), and the bottom ends of the two threaded rods (402) pass through the assembly frame (401) and are fixedly connected to the driven bevel gear (404), a connecting rod (405) is rotatably connected in the assembly frame (401), two driving bevel gears (406) are fixedly connected to the connecting rod (405) and are respectively meshed with the driven bevel gear (404), a worm gear (407) is fixedly connected to the connecting rod (405), an assembly frame (401) is fixedly connected to the assembly frame (408), a servo motor (409) is fixedly connected to the assembly frame (408), a driving end of the servo motor (409) passes through the assembly frame (408) and is fixedly connected to a worm (4010) meshed with the worm gear (407).

6. The stem cell culture device according to claim 1, characterized in that: A heating plate (6) is fixedly connected to the left inner wall of the incubator (1), and a group of ultraviolet sterilization lamps (7) are evenly fixedly connected to the right inner wall of the incubator (1).

7. The stem cell culture device according to claim 1, characterized in that: A temperature sensor (8) is fixedly connected to the rear inner wall of the incubator (1), and a controller (9) is connected to the front wall of the incubator (1). The controller (9) is electrically connected to the temperature sensor (8).

8. The stem cell culture device according to claim 1, characterized in that: A rectangular opening is provided on the front side wall of the incubator (1), and a rectangular transparent plate (10) is sealed and fixedly connected in the rectangular opening.

9. The stem cell culture device according to claim 1, characterized in that: The left and right side walls of the incubator (1) are both fixedly connected with handles (11), and the handles (11) are both fixedly sleeved with rubber sleeves.

10. The stem cell culture device according to claim 1, characterized in that: The bottom of the incubator (1) is fixedly connected to an anti-skid pad (12), and the bottom surface of the anti-skid pad (12) is provided with anti-skid patterns.

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