High-flux full-automatic nucleic acid extraction and purification device

Abstract

The invention discloses a high-flux full-automatic nucleic acid extraction and purification device which comprises a shell, a frame assembly, a translation assembly and a mechanical arm assembly, the frame assembly is arranged in the shell, at least one group of stations are arranged at the bottom of the frame assembly, and a plurality of placing grooves for placing kits are formed in each group of stations; the translation assembly is arranged at the top of the frame assembly; the mechanical arm assembly is fixedly connected to the translation assembly, and the mechanical arm assembly can be driven by the translation assembly to reciprocate on the frame assembly; a magnetic bar clamping end and a magnetic bar sleeve clamping end are formed on the mechanical arm assembly, the magnetic bar clamping end, the magnetic bar sleeve clamping end and any group of stations are correspondingly arranged, and the magnetic bar clamping end or the magnetic bar sleeve clamping end can lift and move on the mechanical arm assembly, so that the corresponding magnetic bar is sleeved with the magnetic bar sleeve.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of nucleic acid extraction device, and particularly relates to a high-throughput full-automatic nucleic acid extraction and purification device. BACKGROUND

[0002] The nucleic acid extraction and purification device is a laboratory instrument for realizing sample nucleic acid extraction, is widely applied to the fields of clinical diagnosis, genomics, epidemic disease monitoring and forensic science, can purify nucleic acid from various biological samples such as whole blood, virus, tissue, plant, bacteria and cultured cells, and provides a high-efficiency, automatic, high-quality nucleic acid purification solution for a laboratory, and serves downstream genetic analysis and molecular diagnosis.

[0003] However, the existing nucleic acid extraction and purification device mainly installs the magnetic rod sleeve manually, which affects experimental efficiency, safety and result reliability, and specifically includes the following aspects: 1. Manual installation needs to be operated one by one manually, especially when a large number of samples are processed, the process is significantly slowed down, the experimental efficiency is low, and the automatic equipment needs to be paused for manual intervention, thereby reducing the overall throughput; 2. When manually installing, gloves or tools may touch the inner wall of the magnetic rod sleeve or the magnetic rod, thereby introducing exogenous pollutants and causing sample contamination; 3. Manual installation may cause the magnetic rod sleeve and the magnetic rod to be attached with different tightness, thereby affecting the magnetic bead adsorption efficiency; and 4. Manual installation may miss installing the magnetic rod sleeve or installing a non-matching model, thereby causing experimental failure or equipment damage. SUMMARY

[0004] In view of the above problems in the prior art, the present application provides a high-throughput full-automatic nucleic acid extraction and purification device to solve the technical problems of low experimental efficiency, poor safety and experimental data reliability of the existing nucleic acid extraction equipment by manually installing the magnetic rod sleeve.

[0005] To achieve the above technical purposes, the present application adopts the following technical solutions: A high-throughput full-automatic nucleic acid extraction and purification device, comprising: A high-throughput full-automatic nucleic acid extraction and purification device, characterized by comprising: a housing; a frame assembly arranged in the housing, a bottom of the frame assembly being arranged with at least one group of working positions, each group of working positions being formed with a plurality of placement grooves for placing reagent boxes; a translation assembly arranged at a top of the frame assembly; A mechanical arm assembly is fixedly connected to the translation assembly, and can reciprocate on the frame assembly under the driving of the translation assembly; the mechanical arm assembly is formed with a magnetic rod clamping end and a magnetic rod sleeve clamping end, which are correspondingly arranged with any group of workstations, and the magnetic rod clamping end or the magnetic rod sleeve clamping end can move up and down on the mechanical arm assembly to enable the magnetic rod sleeve to be sleeved outside the corresponding magnetic rod.

[0006] In some embodiments, the mechanical arm assembly comprises a mounting rack, a magnetic rod clamping unit, a magnetic rod sleeve clamping unit, a first driving unit and a second driving unit, the mounting rack is fixed at the top of the translation assembly, the magnetic rod clamping unit and the magnetic rod sleeve clamping unit are fixed in sequence along the height direction of the mounting rack, the magnetic rod clamping unit constitutes the magnetic rod clamping end, the magnetic rod sleeve clamping unit constitutes the magnetic rod sleeve clamping end, the first driving unit and the second driving unit are arranged on the mounting rack, the first driving unit is connected with the magnetic rod clamping unit, and the second driving unit is connected with the magnetic rod sleeve clamping unit.

[0007] In some embodiments, the mechanical arm assembly further comprises a guide rail unit, the guide rail unit is arranged on both sides of the mounting rack, and the two ends of the magnetic rod clamping unit and the magnetic rod sleeve clamping unit are respectively connected to the guide rail unit in a sliding manner.

[0008] In some embodiments, the magnetic rod clamping unit comprises a magnetic rod fixing plate, a magnetic rod pressing plate, a magnetic rod reinforcing plate and a magnetic rod sliding block, two magnetic rod sliding blocks are respectively fixed on both sides of the magnetic rod fixing plate and connected to the guide rail unit in a matched manner, the magnetic rod reinforcing plate is arranged on the top of the magnetic rod fixing plate, a plurality of magnetic rod pressing plates are detachably connected to the bottom of the magnetic rod fixing plate according to the corresponding workstations, and a plurality of magnetic rods are adhered to the magnetic rod pressing plates.

[0009] In some embodiments, the magnetic rod sleeve clamping unit comprises a magnetic rod sleeve fixing plate, a magnetic rod sleeve pressing plate, a magnetic rod sleeve reinforcing plate, a magnetic rod sleeve supporting plate and a magnetic rod sleeve sliding block, two magnetic rod sleeve sliding blocks are respectively fixed on both sides of the magnetic rod sleeve fixing plate and connected to the guide rail unit in a matched manner, the magnetic rod sleeve reinforcing plate and the magnetic rod sleeve supporting plate are respectively arranged on both sides of the top of the magnetic rod sleeve fixing plate, a plurality of magnetic rod sleeve pressing plates are detachably connected to the bottom of the magnetic rod sleeve fixing plate according to the corresponding workstations, and a plurality of magnetic rod sleeves are arranged on the magnetic rod sleeve pressing plates.

[0010] In some embodiments, the robotic arm assembly further includes a pick-and-place unit, which includes a push rod motor, a hanging plate, a cam, a tension spring, and a hook. The push rod motor is fixed to the magnetic rod sleeve support plate, the hanging plate is connected to the push rod motor, the cam is disposed on the side of the hanging plate away from the push rod motor, the hook is connected to the cam, and the tension spring is disposed between the two cams.

[0011] In some embodiments, a heating component is also included, which is disposed at one of the workstations and located at the bottom of the reagent kit for heating the reagent kit.

[0012] In some embodiments, the heating assembly includes a mounting base plate, a heating base plate, a heat insulation frame, and a heating block. The mounting base plate is disposed in the placement slot, the heating base plate and the heat insulation frame are sequentially disposed on the mounting base plate, and the heating block is embedded in the heat insulation frame and disposed at the bottom of the reagent kit for heating the reagent kit.

[0013] In some embodiments, the heating assembly further includes a temperature sensor and a temperature control switch, the temperature sensor and the temperature control switch being mounted on the heating base plate, and the temperature sensor being signal-connected to the temperature control switch.

[0014] In some embodiments, a limiting component is further included, the limiting component including a plurality of limiting blocks, the plurality of limiting blocks being respectively disposed at the four corners of the placement slot.

[0015] Compared with the prior art, the beneficial effects of the present invention mainly include: This invention provides a high-throughput fully automated nucleic acid extraction and purification device. By incorporating a robotic arm assembly and a translational assembly, the robotic arm assembly can move along the X-axis under the drive of the translational assembly to accommodate reagent kits at different workstations. Furthermore, the robotic arm assembly designed in this invention includes a magnetic rod clamping end and a magnetic rod sleeve clamping end, respectively for mounting magnetic rods and magnetic rod sleeves. The magnetic rod clamping end or magnetic rod sleeve clamping end can move vertically and vertically on the robotic arm assembly. This allows for automatic placement of the magnetic rod sleeve on the outside of the magnetic rod, achieving automated installation, improving experimental efficiency, avoiding manual intervention, preventing sample contamination, and improving the accuracy of experimental results. Attached Figure Description

[0016] Figure 1 This is a schematic diagram of the overall structure of the extraction and purification device described in this invention; Figure 2 yes Figure 1 A schematic diagram of the structure without the outer shell; Figure 3 This is a schematic diagram of the structure of the robotic arm assembly described in this invention; Figure 4This is a schematic diagram of the structure of the magnetic rod clamping unit described in this invention; Figure 5 This is a schematic diagram of the magnetic rod sleeve clamping unit described in this invention; Figure 6 This is another structural schematic diagram of the magnetic rod sleeve clamping unit described in this invention; Figure 7 This is a schematic diagram of the structure of the heating assembly described in this invention; Figure 8 This is a schematic diagram of the structure of the reagent kit described in this invention placed on the heating assembly; Figure 9 This is a schematic diagram of the filter assembly of the present invention installed on the housing.

[0017] Explanation of reference numerals in the attached figures: 100. Outer shell; 110. Main body; 120. Flip-down door; 130. Touch screen; 200, Frame assembly; 210, Base plate; 220, First side plate; 230, Top plate; 240, Second side plate; 300. Translation assembly; 310. Linear guide rail; 320. Drive motor; 330. Synchronous pulley; 340. Synchronous belt; 350. Idler pulley. 400. Robotic arm assembly; 410. Mounting bracket; 420. Magnetic rod clamping unit; 421. Magnetic rod fixing plate; 422. Magnetic rod pressure plate; 423. Magnetic rod reinforcing plate; 424. Magnetic rod slider; 425. Magnetic rod; 430. Magnetic rod sleeve clamping unit; 431. Magnetic rod sleeve fixing plate; 432. Magnetic rod sleeve pressure plate; 433. Magnetic rod sleeve reinforcing plate; 434. Magnetic rod sleeve support plate; 435. Magnetic rod sleeve slider; 436. Magnetic rod sleeve; 440. First drive unit; 450. Second drive unit; 460. Guide rail unit; 470. Pick-and-place unit; 471. Push rod motor; 472. Hanging plate; 473. Cam; 474. Tension spring; 475. Hook; 500. Heating component; 510. Mounting base plate; 520. Heating base plate; 530. Heat insulation frame; 540. Heating block; 550. Silicone pad; 560. Pressure plate. 600. Reagent kit; 700, Limiting components; 800. Filter assembly; 810. Axial flow fan; 820. Filter membrane; 830. Filter cover. Detailed Implementation

[0018] To make the objectives, technical solutions, and advantages of this invention clearer, the invention will be further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative and not intended to limit the invention.

[0019] Please see Figure 1 and Figure 2 As shown, this invention provides a high-throughput fully automated nucleic acid extraction and purification device, including a shell 100, a frame assembly 200, a translation assembly 300, and a robotic arm assembly 400. The frame assembly 200 is disposed within the shell 100, and at least one set of workstations (workstations 1-6 are provided at the bottom of the frame assembly 200) are arranged, with each set of workstations forming multiple placement slots for placing reagent kits 600. The translation assembly 300 is disposed on top of the frame assembly 200, and the robotic arm assembly 400 is fixedly connected to the translation assembly. 300, then the robotic arm assembly 400 can reciprocate on the frame assembly 200 under the drive of the translation assembly 300, so as to gradually move from station 1 to station 6 or from station 6 to station 1; the robotic arm assembly 400 has a magnetic rod clamping end and a magnetic rod sleeve clamping end, the magnetic rod clamping end and the magnetic rod sleeve clamping end are correspondingly arranged with any set of stations, the magnetic rod clamping end or the magnetic rod sleeve clamping end can be lifted and lowered on the robotic arm assembly 400 so that the magnetic rod sleeve is sleeved on the outside of the corresponding magnetic rod.

[0020] The high-throughput fully automated nucleic acid extraction and purification device provided by this invention enables the robotic arm assembly 400 to move along the X-axis under the drive of the translation component 300, accommodating reagent kits at different workstations. Simultaneously, the robotic arm assembly 400 designed in this invention includes a magnetic rod clamping end and a magnetic rod sleeve clamping end for mounting magnetic rods and magnetic rod sleeves, respectively. The magnetic rod clamping end or magnetic rod sleeve clamping end can move vertically and vertically on the robotic arm assembly 400, thus automatically mounting the magnetic rod sleeve onto the outside of the magnetic rod, achieving automated installation of the magnetic rod sleeve, improving experimental efficiency, avoiding manual intervention, preventing sample contamination, and improving the accuracy of experimental results.

[0021] In addition, each workstation of the present invention is equipped with four placement slots, and each placement slot can hold a 96-well plate reagent kit, that is, it can process 96 samples. Therefore, each workstation can hold four reagent kits, which means it can process 384 samples at a time, and can cope with the detection of large-scale samples.

[0022] The specific structure of the nucleic acid extraction and purification device provided by the present invention will be described in detail below.

[0023] In one embodiment, such as Figure 1 As shown, the outer casing 100 includes a main body 110 and a flip-down door 120. The main body 110 has a rectangular structure, and the frame assembly 200 is disposed in the internal space of the main body 110. The flip-down door 120 is disposed on one side of the main body 110. One end of the flip-down door 120 is hinged to the main body 110, and the other end can rotate around the hinge end to open or close the internal space of the main body 110.

[0024] In one embodiment, a touch screen 130 is also provided on the main body 110.

[0025] In one embodiment, such as Figure 2 As shown, the frame assembly 200 is a gantry frame structure composed of a base plate 210, a first side plate 220, a top plate 230 and a second side plate 240 tightened together with screws. Workstations 1-6 are arranged on the base plate 210, and the translation assembly 300 is set on the top plate 230.

[0026] In one embodiment, a drag chain is also provided on the top plate 230 to connect the power lines and signal lines of the touch screen 122, various drive motors, ultraviolet lamps, fans and lighting lamps to the main control board.

[0027] In one embodiment, the translation component 300 comprises a linear guide rail 310, a drive motor 320, a synchronous pulley 330, a synchronous belt 340, an idler pulley 350, and a connecting block (not shown in the figure). The two linear guide rails 310 are arranged on both sides of the top plate 230. The drive motor 320 is mounted on the top plate 230. The synchronous pulley 330 is connected to the drive motor 320. The idler pulley 350 is mounted on the side of the top plate 230 opposite to the synchronous pulley 330. The synchronous belt 340 is simultaneously fitted onto the synchronous pulley 330 and the idler pulley 350. The two ends of the connecting block are respectively connected to the synchronous belt 340 and the robotic arm component 400, so as to drive the robotic arm component 400 to move on the two linear guide rails 310, thereby driving the magnetic rod clamping end and the magnetic rod sleeve clamping end to move from station 1 to station 6.

[0028] In one embodiment, such as Figure 3As shown, the robotic arm assembly 400 includes a mounting frame 410, a magnetic rod clamping unit 420, a magnetic rod sleeve clamping unit 430, a first drive unit 440, and a second drive unit 450. The mounting frame 410 is a frame structure with an open bottom, formed by a top surface and two side panels. The top of the mounting frame 410 is fixed to the translation assembly 300, specifically to the first connecting block. The top panel of the mounting frame 410 forms a groove that mates with the first linear guide rail 310, allowing the robotic arm assembly 400 to move on the frame assembly 200 through the groove and the first linear guide rail 310. The magnetic rod clamping unit 420 and the magnetic rod sleeve clamping unit 430... The magnetic rod clamping unit 420 is fixed sequentially on the mounting frame 410 along the height direction. The magnetic rod clamping unit 430 forms the magnetic rod clamping end. The first driving unit 440 and the second driving unit 450 are respectively disposed on the top of the mounting frame 410. The first driving unit 440 is connected to the magnetic rod clamping unit 420 to drive the magnetic rod clamping unit 420 to move up and down on the mounting frame 410. The second driving unit 450 is connected to the magnetic rod clamping unit 430 to drive the magnetic rod clamping unit 430 to move up and down on the mounting frame 410.

[0029] In one embodiment, the robotic arm assembly 400 further includes a guide rail unit 460, which is respectively disposed on both sides of the mounting bracket 410. The two ends of the magnetic rod clamping unit 420 and the magnetic rod sleeve clamping unit 430 are slidably connected to the guide rail unit 460.

[0030] In one embodiment, such as Figure 4 As shown, the magnetic rod clamping unit 420 includes a magnetic rod fixing plate 421, a magnetic rod pressure plate 422, a magnetic rod reinforcing plate 423, and a magnetic rod slider 424. Two magnetic rod sliders 424 are respectively fixed on both sides of the magnetic rod fixing plate 421 and are connected to the guide rail unit 460. The magnetic rod reinforcing plate 423 is disposed on the top of the magnetic rod fixing plate 421. Multiple magnetic rod pressure plates 422 are detachably connected to the bottom of the magnetic rod fixing plate 421 according to the corresponding work positions. Multiple magnetic rods 425 are bonded to the magnetic rod pressure plate 422.

[0031] In one embodiment, such as Figure 5 and Figure 6As shown, the magnetic rod sleeve clamping unit 430 includes a magnetic rod sleeve fixing plate 431, a magnetic rod sleeve pressure plate 432, a magnetic rod sleeve reinforcing plate 433, a magnetic rod sleeve support plate 434, and a magnetic rod sleeve slider 435. Two magnetic rod sleeve sliders 435 are respectively fixed on both sides of the magnetic rod sleeve fixing plate 431 and are connected to the guide rail unit 460. The magnetic rod sleeve reinforcing plate 433 and the magnetic rod sleeve support plate 434 are respectively disposed on the top sides of the magnetic rod sleeve fixing plate 431. Multiple magnetic rod sleeve pressure plates 432 are detachably connected to the bottom of the magnetic rod sleeve fixing plate 431 according to the corresponding work positions. Multiple magnetic rod sleeves 436 are disposed on the magnetic rod sleeve pressure plate 432.

[0032] In one embodiment, the first drive unit 440 is used to drive the magnetic rod clamping unit 420 to move up and down on the mounting frame 410. The first drive unit 440 adopts the form of a linear motor and a threaded rod. The linear motor is installed on the top of the mounting frame 410, and the threaded rod is connected to the linear motor and is connected to the threaded hole of the magnetic rod fixing plate 421 of the magnetic rod clamping unit 420. The second drive unit 450 is used to drive the magnetic rod sleeve clamping unit 430 to move up and down on the mounting frame 410. The second drive unit 450 adopts the form of a drive motor and a synchronous belt. The drive motor is installed on the top of the mounting frame 410, and the idler wheel is installed on the magnetic rod sleeve fixing plate 431 of the magnetic rod sleeve clamping unit 430. The synchronous belt is simultaneously sleeved on the idler wheel and the synchronous wheel. The working principle of the second drive unit 450 is the same as that of the translation component 300. At the two ends of the magnetic rod fixing plate 421 and the magnetic rod sleeve fixing plate 431, there are also grooves that cooperate with the guide rail of the guide rail unit 460.

[0033] In one embodiment, the robotic arm assembly 400 further includes a pick-and-place unit 470, which includes a push rod motor 471, a hanging plate 472, a cam 473, a tension spring 474, and a hook 475. The push rod motor 471 is fixed to the magnetic rod sleeve support plate 434, the hanging plate 472 is connected to the push rod motor 471, the cam 473 is disposed on the side of the hanging plate 472 away from the push rod motor 471, the hook 475 is connected to the cam 473, and the tension spring 474 is disposed between the two cams 473.

[0034] In the above technical solution, the magnetic rod sleeve fixing plate 431 can hold a total of 4 magnetic rod sleeves 436 of reagent kits. The pick-and-place unit 470 is driven by the push rod motor 471 to rotate the cam 473, which in turn drives the hook 475 to rotate. Under the action of the tension spring 474, the hook of the hook 475 can clamp or release the protrusion of the magnetic rod 425, clamping the magnetic rod sleeve on the magnetic rod or releasing it.

[0035] In one embodiment, the pick-and-place unit 470 further includes a micro switch that is triggered when the magnetic rod sleeve falls off and an alarm is issued through the control software.

[0036] In one embodiment, the nucleic acid extraction and purification device provided by the present invention further includes a heating component 500, which is disposed on one of the workstations and located at the bottom of the reagent kit 600, for heating the reagent kit 600.

[0037] In one embodiment, such as Figure 7 As shown, the heating assembly 500 includes a mounting base plate 510, a heating base plate 520, a heat insulation frame 530, and a heating block 540. The mounting base plate 510 is disposed in the placement groove. The heating base plate 520 and the heat insulation frame 530 are sequentially disposed on the mounting base plate 510. The heating block 540 is embedded in the heat insulation frame 530 and disposed at the bottom of the reagent kit 600 for heating the reagent kit 600. The heating block 540 can be replaced with different heating blocks according to different types of reagent kit 600.

[0038] In one embodiment, the heating assembly 500 further includes a temperature sensor and a temperature control switch, which are mounted on the heating base plate 520, and the temperature sensor is signal-connected to the temperature control switch.

[0039] In one embodiment, a silicone pad 550 and a pressure plate 560 are also provided between the mounting base plate 510 and the heating base plate 520.

[0040] In one embodiment, such as Figure 8 As shown, the nucleic acid extraction and purification device provided by the present invention also includes a limiting component 700. The limiting component 700 includes multiple limiting blocks, which are respectively disposed at the four corners of the placement groove for positioning and installing the reagent kit 600. The limiting blocks are designed with hole mounting ball screws on both sides. The ball screws have spring-loaded steel balls. When the reagent kit 600 is placed, the steel balls on the ball screws are locked at the bottom of the reagent kit 600, which serves to hold the main reagent kit 600 in place and prevent the reagent kit 600 from being lifted up by the up-and-down vibration of the magnetic rod sleeve during the nucleic acid extraction process. At the same time, a large C-angle is designed on the reagent kit 600, which can serve as a guide when the reagent kit 600 is placed.

[0041] In one embodiment, such as Figure 9As shown, the nucleic acid extraction and purification device provided by the present invention further includes a high-efficiency filter assembly 800. A mounting hole is formed on the top of the outer shell 100, and the filter assembly 800 is installed in the mounting hole. Further, the filter assembly 800 includes an axial flow fan 810, a filter membrane 820, and a filter cover 830. The axial flow fan 810 is disposed on the inner wall of the outer shell 100 at the mounting hole. The filter membrane 820 and the filter cover 830 are sequentially arranged in the mounting hole from the direction closest to the axial flow fan 810 to the direction away from the axial flow fan 810, so as to draw air from inside the nucleic acid extraction and purification device, and discharge the contaminated gas inside the device after passing through the filter membrane 820. The filter cover 830 is detachably connected to the mounting hole, facilitating periodic replacement of the filter membrane 820.

[0042] In summary, the high-throughput fully automated nucleic acid extraction and purification device provided by this invention has the following beneficial effects: 1. This invention can process 96 / 192 / 288 / 384 samples, offering high flexibility and enabling it to handle the detection of large-scale samples; 2. This invention realizes the automatic installation process of the magnetic rod sleeve, which improves experimental efficiency, reduces manual intervention, and ensures the accuracy of experimental data; 3. The present invention allows for the replacement of different heating structures depending on the type of reagent kit, which helps to improve the heating rate and temperature stability; 4. The reagent kit station of the present invention has elasticity and guiding function, and can be used with an external robotic arm to realize automatic loading and unloading.

[0043] The specific embodiments of the present invention described above do not constitute a limitation on the scope of protection of the present invention. Any other corresponding changes and modifications made in accordance with the technical concept of the present invention should be included within the scope of protection of the claims of the present invention.

Claims

1. A high-throughput fully automated nucleic acid extraction and purification device, characterized in that, include: shell; A frame assembly is disposed within the housing, and at least one set of workstations is arranged at the bottom of the frame assembly, each set of workstations forming multiple placement slots for placing reagent kits; A translation component is disposed on top of the frame component; A robotic arm assembly is fixedly connected to the translation assembly. The robotic arm assembly can reciprocate on the frame assembly under the drive of the translation assembly. The robotic arm assembly has a magnetic rod clamping end and a magnetic rod sleeve clamping end. The magnetic rod clamping end, the magnetic rod sleeve clamping end and any set of workstations are correspondingly arranged. The magnetic rod clamping end or the magnetic rod sleeve clamping end can be lifted and lowered on the robotic arm assembly so that the magnetic rod sleeve is fitted on the outside of the corresponding magnetic rod.

2. The high-throughput fully automated nucleic acid extraction and purification device according to claim 1, characterized in that, The robotic arm assembly includes a mounting frame, a magnetic rod clamping unit, a magnetic rod sleeve clamping unit, a first drive unit, and a second drive unit. The top of the mounting frame is fixed to the translation assembly. The magnetic rod clamping unit and the magnetic rod sleeve clamping unit are sequentially fixed to the mounting frame along the height direction of the mounting frame. The magnetic rod clamping unit constitutes the magnetic rod clamping end, and the magnetic rod sleeve clamping unit constitutes the magnetic rod sleeve clamping end. The first drive unit and the second drive unit are respectively disposed on the mounting frame. The first drive unit is connected to the magnetic rod clamping unit, and the second drive unit is connected to the magnetic rod sleeve clamping unit.

3. The high-throughput fully automated nucleic acid extraction and purification device according to claim 2, characterized in that, The robotic arm assembly also includes guide rail units, which are respectively disposed on both sides of the mounting frame. The two ends of the magnetic rod clamping unit and the magnetic rod sleeve clamping unit are slidably connected to the guide rail units.

4. The high-throughput fully automated nucleic acid extraction and purification device according to claim 3, characterized in that, The magnetic rod clamping unit includes a magnetic rod fixing plate, a magnetic rod pressure plate, a magnetic rod reinforcing plate, and a magnetic rod slider. Two magnetic rod sliders are respectively fixed on both sides of the magnetic rod fixing plate and are connected to the guide rail unit. The magnetic rod reinforcing plate is disposed on the top of the magnetic rod fixing plate. Multiple magnetic rod pressure plates are detachably connected to the bottom of the magnetic rod fixing plate according to the corresponding work positions. Multiple magnetic rods are bonded to the magnetic rod pressure plates.

5. The high-throughput fully automated nucleic acid extraction and purification device according to claim 3, characterized in that, The magnetic rod sleeve clamping unit includes a magnetic rod sleeve fixing plate, a magnetic rod sleeve pressure plate, a magnetic rod sleeve reinforcing plate, a magnetic rod sleeve support plate, and a magnetic rod sleeve slider. Two magnetic rod sleeve sliders are respectively fixed on both sides of the magnetic rod sleeve fixing plate and are connected to the guide rail unit. The magnetic rod sleeve reinforcing plate and the magnetic rod sleeve support plate are respectively disposed on the top sides of the magnetic rod sleeve fixing plate. Multiple magnetic rod sleeve pressure plates are detachably connected to the bottom of the magnetic rod sleeve fixing plate according to the corresponding work positions. Multiple magnetic rod sleeves are disposed on the magnetic rod sleeve pressure plates.

6. The high-throughput fully automated nucleic acid extraction and purification device according to claim 5, characterized in that, The robotic arm assembly also includes a pick-and-place unit, which includes a push rod motor, a hanging plate, a cam, a tension spring, and a hook. The push rod motor is fixed to the magnetic rod sleeve support plate, the hanging plate is connected to the push rod motor, the cam is located on the side of the hanging plate away from the push rod motor, the hook is connected to the cam, and the tension spring is located between the two cams.

7. The high-throughput fully automated nucleic acid extraction and purification device according to claim 1, characterized in that, It also includes a heating component, which is disposed on one of the workstations and located at the bottom of the reagent kit, for heating the reagent kit.

8. The high-throughput fully automated nucleic acid extraction and purification device according to claim 7, characterized in that, The heating assembly includes a mounting base plate, a heating base plate, a heat insulation frame, and a heating block. The mounting base plate is disposed in the placement slot, the heating base plate and the heat insulation frame are sequentially disposed on the mounting base plate, and the heating block is embedded in the heat insulation frame and disposed at the bottom of the reagent kit for heating the reagent kit.

9. The high-throughput fully automated nucleic acid extraction and purification device according to claim 8, characterized in that, The heating assembly also includes a temperature sensor and a temperature control switch, which are mounted on the heating base plate, and the temperature sensor is signal-connected to the temperature control switch.

10. The high-throughput fully automated nucleic acid extraction and purification device according to claim 1, characterized in that, It also includes a limiting component, which includes multiple limiting blocks, each of which is disposed at one of the four corners of the placement slot.

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