Simulation system for accurately controlling synchronous collection and culture process of sediment-water-gas samples

Abstract

The invention relates to a simulation system for accurately controlling the synchronous collection and culture process of sediment-water-gas samples, in particular to a device for indoor culture and sample collection of sediment, which mainly comprises a culture module, a sampling module and a control module. The defect that sediments, water bodies and gas samples in the sediment culture process are difficult to synchronously collect for a long time in the prior art is overcome, the consistency and the stability of the water-sediment proportion in a culture system can be ensured, and errors caused by experiments are reduced. The method is of great significance in improving the experiment efficiency and reducing experiment errors and the workload of experimenters.

Description

Technical Field

[0001] This invention relates to the field of laboratory sediment culture and sampling technology, specifically to a simulation system for the precise control of the synchronous collection and culture process of sediment-water-gas samples. Background Technology

[0002] Sediment culture experiments are a key technique for studying biogeochemical cycling processes in sediments of aquatic ecosystems such as rivers, lakes, and oceans. They can effectively reveal important scientific questions such as the dynamic changes of sediment microbial communities, the mechanisms of biogenic element transformation, and the patterns of greenhouse gas release. This has significant scientific value for a deeper understanding of carbon and nitrogen cycling processes and for assessing the impacts of climate change. To achieve these research objectives, it is necessary to establish an experimental setup capable of maintaining the stability of the sediment-water system over the long term and enabling simultaneous sampling of multiple media (sediments, water, and gases).

[0003] Existing technologies mainly employ the following two cultivation methods: first, directly culturing raw sediment columns collected in the field; second, transferring sediment samples to containers such as beakers, glass tanks, or headspace bottles for cultivation.

[0004] When multi-media sampling is required, the existing method requires temporary sealing of the culture container and then sampling by puncture with a syringe. However, this operation method has the following inherent defects: (1) it cannot guarantee the time synchronization of sampling of multiple parallel culture systems; (2) it is difficult to maintain the stability of the water-sediment interface during long-term continuous sampling; (3) the sampling process is prone to introducing external interference factors.

[0005] In summary, the existing technology has the following main shortcomings:

[0006] (1) There is a lack of integrated devices that can simultaneously collect sediment phase, water phase and gas phase samples, that is, the system integration is insufficient;

[0007] (2) Parallel control defects make it impossible to achieve time-series synchronous sampling of multiple independent culture systems;

[0008] (3) In large-volume culture systems, continuous sampling can lead to an imbalance in the water-sediment ratio, making it impossible to maintain a constant interface height.

[0009] Therefore, there is an urgent need for a simulation system that can achieve simultaneous collection and precise control of the three-phase media of sediment-water-gas to address the shortcomings of existing technologies. Summary of the Invention

[0010] The purpose of this invention is to address the problems existing in the prior art by providing a simulation system for the simultaneous acquisition and precise control of sediment-water-gas sample cultivation process, which enables the simultaneous acquisition and precise control of sediment-water-gas three-phase media.

[0011] To address the problems existing in the prior art, the present invention adopts the following technical solution:

[0012] A simulation system for the simultaneous collection and precise control of sediment-water-gas sample cultivation process includes a cultivation module, a sampling module, and a control module;

[0013] The culture module includes a culture chamber for storing sediments and water, and a culture environment is formed inside the culture chamber.

[0014] The sampling module includes a sampling body, which is provided with a collection mechanism and multiple through holes for injecting gas or sampling. The collection mechanism includes blocking components corresponding to the multiple through holes. The control module includes a first drive mechanism connected to the blocking components to switch the opening and closing of the through holes through the blocking components.

[0015] The sampling body is set in the culture environment, and the first driving mechanism controls the shielding component to switch the opening and closing of the through hole for synchronous sampling.

[0016] As an improvement to the technical solution of the simulation system for the precise control of the synchronous collection and cultivation process of sediment-water-gas samples of the present invention, the sampling body is provided with a drainage hole, a water collection hole and a gas collection hole on its side wall. The drainage hole and the water collection hole are located in the lower part of the side wall, and the gas collection hole is located in the middle and upper part of the side wall.

[0017] The sampling body has elongated through holes on its opposite side walls, and two shielding components are correspondingly arranged at the elongated through holes; the bottom of the sampling body has a fully open through hole, and the top has a first through hole for inserting a connecting pipe.

[0018] One end of the connecting pipe passes through the first through hole and is connected to the first sealing component, while the other end is connected to the solenoid valve.

[0019] As an improvement to the technical solution of the simulation system for precise control of the synchronous collection and cultivation process of sediment-water-gas samples of the present invention, the first driving mechanism includes pulley groups respectively arranged above two elongated through holes and a first power source assembly, with each pulley group arranged above one of the elongated through holes;

[0020] The first power source assembly includes a first motor, and the main shaft of the first motor is connected to the transmission rod through a transmission gear;

[0021] Each pulley assembly includes a pulley bracket on which a pulley is mounted. A cable is wound around the pulley. The pulley is connected to a transmission gear via a transmission rod. One end of the cable is connected to the shielding component, and the other end of the cable is connected to the transmission rod.

[0022] As an improvement to the technical solution of the simulation system for the synchronous collection and precise control of sediment-water-gas sample cultivation process of the present invention, the cultivation module includes a movable plate erected in the cultivation chamber. The movable plate is connected to the first driving mechanism, which drives the movable plate to move along the length direction of the cultivation chamber. After each sampling, the movement of the movable plate maintains the height ratio of water to sediment in the cultivation chamber.

[0023] As an improvement to the technical solution of the simulation system for the synchronous collection and precise control of sediment-water-gas sample cultivation process of the present invention, the cultivation module includes a water circulation system connected to the cultivation chamber.

[0024] As an improvement to the technical solution of the simulation system for precise control of the synchronous collection and culture process of sediment-water-gas samples of the present invention, the control module includes a support frame and a control system.

[0025] The support bracket includes a bottom bracket, on which a lifting bracket and a moving bracket are provided for controlling and adjusting the height and horizontal position of the sampling module. The moving bracket is mounted on the lifting bracket, and the sampling module is mounted on the moving bracket.

[0026] As an improvement to the technical solution of the simulation system for precise control of the synchronous collection and culture process of sediment-water-gas samples of the present invention, the bottom support is provided with multiple syringes for collecting water and gas samples respectively, and the lower part of the bottom support is provided with multiple slots for placing and storing centrifuge tubes for water samples, turbid water and gas samples, hoses for connecting syringes, centrifuge tubes, gas collection bags and sampling modules, three-way valves for controlling the opening and closing of the passage, motors and lead screws for controlling the operation of syringes.

[0027] The beneficial effects of this invention are:

[0028] 1. The simulation system for simultaneous collection and precise control of sediment-water-gas sample cultivation process of the present invention can realize the simultaneous and rapid sampling of sediment, water and gas samples, while maintaining a stable water-sediment height ratio in the cultivation tank and avoiding the impact of water level changes on gas collection.

[0029] 2. The rapid sampling process of this invention significantly saves sampling time and reduces errors in experimental results caused by the operation.

[0030] 3. The present invention adopts a modular combination design. The sampling module and control module of the present invention are small in size, so multiple modules can be used in parallel. Multiple sampling modules and control modules can be controlled by one control box to work simultaneously and sample one or more culture systems at the same time.

[0031] 4. This invention supports manual sampling using only the culture module and the acquisition module, meeting different experimental needs and greatly improving flexibility. Attached Figure Description

[0032] Figure 1 This is a schematic diagram of the overall structure of the present invention;

[0033] Figure 2 This is a schematic diagram of the cultivation module in this invention;

[0034] Figure 3 This is a schematic diagram of the sampling module in this invention, wherein... Figure 3 (a) is a structural diagram of the sampling subject from one angle. Figure 3 (b) is a structural diagram of the sampling subject from another angle. Figure 3 (c) is a schematic diagram of the internal structure of the sampling subject. Figure 3 (d) is a schematic diagram of the pulley system;

[0035] Figure 4 This is a schematic diagram of the control module in this invention;

[0036] Figure 5 This is a schematic diagram showing the detailed structure of the control module bracket in this invention;

[0037] Explanation of reference numerals in the attached figures:

[0038] A-Cultivation Module: A01-Cultivation Cylinder; A02-Moving Plate; A03-Sealing Strip; A04-Plastic Tube; A05-Circulation Pump; A06-Moving Rod; A07-Rotator; A08-Sealing Nut; A09-Base;

[0039] B-Sampling Module: B01-Acrylic Body; B02-Drainage Hole; B03-Water Sampling Hole; B04-Air Sampling Hole; B05-Roller Shutter; B06-Cable; B07-Limiting Post; B08-Balloon; B09-Connecting Pipe; B10-Solenoid Valve; B11-Pulley; B12-Pulley Bracket; B13-Transmission Rod; B14-Transmission Gear;

[0040] C-Control Module: C01-Bottom Support; C02-Lifting Support; C03-Lifting Rail; C04-Moving Support; C05-Moving Rail; C06-Lead Screw; C07-Lead Screw Rail; C08-Motor; C09-Instrument; C10-Centrifuge Tube; C11-Hose; C12-Solenoid Valve; C13-Gas Sampling Bag; C14-Control Box. C01-1-Centrifuge Tube Placement Slot; C01-2-Instrument Bottom Fixing Plate; C01-3-Instrument Top Fixing Plate; C01-4-Instrument Moving Motor Fixing Plate; C01-5-Lifting Support Fixing Plate. Detailed Implementation

[0041] To make the objectives, technical solutions, and beneficial effects of this invention clearer, the technical solutions in the embodiments of this invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of this invention, and not all embodiments.

[0042] like Figures 1 to 4 As shown, a simulation system for the simultaneous acquisition and precise control of sediment-water-gas sample cultivation process includes a cultivation module, a sampling module, and a control module.

[0043] The cultivation module includes a cultivation tank for storing sediments and water, forming a cultivation environment within the cultivation tank;

[0044] The sampling module includes a sampling body, which is provided with a collection mechanism and multiple through holes for injecting gas or sampling. The collection mechanism includes shielding components corresponding to the multiple through holes. The control module includes a first drive mechanism connected to the shielding components to switch the opening and closing of the through holes through the shielding components.

[0045] The sampling body is set up in the culture environment, and the first drive mechanism controls the shielding component to switch the opening and closing of the through hole for synchronous sampling.

[0046] Firstly, in this invention, the culture chamber of the culture module provides a stable culture environment for sediments and water. The sampling module's sampling body has multiple through holes and corresponding shielding components. Together with the first drive mechanism in the control module connected to the shielding components, it can realize the synchronous collection of sediment, water, and gas samples. This avoids the sample differences caused by time differences and environmental disturbances when collecting different media samples separately in the traditional way, and ensures the consistency of the three media samples in time and space. This provides more accurate and more correlated data for studying the material exchange, chemical reactions, or ecological processes between them.

[0047] Second, the sampling module is directly set in the culture environment, which can perform sampling dynamically and in real time during the culture process without transferring the sample to other devices, thus reducing the interference of the external environment on the culture system. The first drive mechanism automatically controls the shielding component to cut off the sample collection through the shielding component.

[0048] Third, the multiple through holes can be adapted to different needs such as gas injection, sediment sampling, and water sampling, which improves sampling efficiency and is especially suitable for long-term, dynamic culture experiments.

[0049] In some embodiments of the present invention, a drainage hole, a water collection hole, and a gas collection hole are provided on the side wall of the sampling body. The drainage hole and the water collection hole are located in the lower part of the side wall, and the gas collection hole is located in the middle and upper part of the side wall.

[0050] The sampling body has elongated through holes on its opposite side walls, and two shielding components are set at the elongated through holes. The bottom of the sampling body has a fully open through hole, and the top has a first through hole for inserting a connecting pipe.

[0051] One end of the connecting pipe passes through the first through hole and is connected to the first sealing component, while the other end is connected to the solenoid valve.

[0052] Furthermore, the first drive mechanism includes pulley sets respectively disposed above two elongated through holes and a first power source assembly, with each pulley set disposed above one elongated through hole;

[0053] The first power source assembly includes a first motor, and the main shaft of the first motor is connected to the transmission rod through a transmission gear.

[0054] Each pulley block includes a pulley bracket, on which a pulley is mounted. A cable is wound around the pulley, and the pulley is connected to a transmission gear via a transmission rod. One end of the cable is connected to a shielding component, and the other end of the cable is connected to the transmission rod.

[0055] As a specific embodiment of this method, the sampling body is a square transparent acrylic body with a round hole at the top and a completely open bottom. Three holes are located on the right side of the sampling body: a drainage hole, a water sampling hole, and a gas sampling hole, from bottom to top. Elongated holes are located at the bottom of the front and rear sides, and a circular first through hole is located at the top for a connecting pipe to pass through. One end of the connecting pipe passes through the first through hole and connects to a first sealing component, while the other end connects to a solenoid valve. Preferably, the first sealing component is a balloon, which allows for adjustment of the gas volume by inflating and deflating the balloon, thereby achieving the effect of adjusting the sealing of the upper part of the sampling body.

[0056] In this embodiment, the blocking component is a roller blind made of stainless steel strips and fine steel wires. The roller blind is connected to a first power source assembly via a cable, allowing the first power source assembly to switch the open and closed states of the elongated opening. Furthermore, limit posts are provided above each elongated opening to limit the maximum extent to which the blocking component is open.

[0057] In detail, sampling module B, such as Figure 3 As shown, the sampling body consists of a square transparent acrylic body B01 with a circular first through hole at the top and a fully open bottom. Three holes are located on the right side of the sampling body: a drainage hole B02, a water sampling hole B03, and an air sampling hole B04, from bottom to top. Long, narrow holes are located on the bottom of the front and rear sides, and limit posts B07 are fixed to both the front and rear sides. A roller shutter B05, woven from stainless steel strips and thin steel wire, and two sets of cables B06 connecting the roller shutter B05 are also included.

[0058] The top opening of the acrylic body B01 is fitted with a connecting pipe B09, which connects to a balloon B08 at the bottom and a solenoid valve B10 at the top. Additionally, the top of the acrylic body B01 is equipped with two sets of four pulley brackets B12, one on each side, each bracket holding a pulley B11. Two sets of cables B06 wrap around their corresponding pulley sets once each and connect to the roller shutter B05. The roller shutter passes through an elongated hole at the bottom of the acrylic body B01, and the two sets of pulleys are connected by a transmission rod with an extended end and a drive gear.

[0059] In use, open the solenoid valve B10 on top of the acrylic body B01, rotate the transmission gear B14, and move the roller shutter B05 via the cable so that the roller shutter B05 does not obstruct the fully open hole at the bottom of the acrylic body B01. Then, under the action of the control module C, lower the entire sampling module until the drain hole B02 is close to the water surface but not submerged. At this time, adjust the water inlet valve to fill the acrylic body B01 with inert gas through the drain hole B02 and exhaust the air through the gas sampling port B04. After a period of time, end the exhaust step, adjust the water inlet valve to the appropriate position, and once the preparation is complete, insert the sampling module B into the culture module A, and then close the solenoid valve B10 on top of the acrylic body B01.

[0060] After a period of cultivation, the solenoid valve B10 on top of the acrylic body B01 is opened, and the syringe in control module C is moved to collect gas samples. After the gas sample collection is completed, the solenoid valve B10 on top of the acrylic body B01 is closed, and then the transmission gear B14 is rotated, which pulls the roller shutter B05 again through the cable B06 to cut the sediment and seal the sediment and water in the acrylic body B01.

[0061] Then, under the control of module C, the entire sampling system is lifted to the designated height. Using the syringe in module C, water samples are collected and water containing sediment and turbidity is removed. Finally, the sediment sample storage container is placed at the bottom of the acrylic body B01, the top solenoid valve B10 is opened, and the transmission gear is rotated to move the traction cable B06, releasing the sediment sample into the container.

[0062] In some embodiments of the present invention, the culture module includes a movable plate erected in the culture chamber, the movable plate being connected to a first driving mechanism, the first driving mechanism driving the movable plate to move along the length direction of the culture chamber; after each sampling, the movement of the movable plate is used to maintain the height ratio of water to sediment in the culture chamber.

[0063] Furthermore, the cultivation module includes a water circulation system connected to the cultivation chamber.

[0064] As a specific embodiment of this implementation, the control module C is as follows: Figure 4 As shown, it includes the bottom bracket C01 (see details). Figure 5 The bottom bracket C01 extends to the left at its lowest point to increase the stability of the entire module.

[0065] From bottom to top, the following components are installed: centrifuge tube placement slot C01-1, syringe bottom fixing plate C01-2, syringe top fixing plate C01-3, syringe moving motor fixing plate C01-4, and lifting bracket fixing plate C01-5.

[0066] Furthermore, the support frame has a hollow structure, allowing the lifting slide rail C03 to move within it. The lower part of the lifting support frame C02 is composed of a metal plate and the lifting slide rail C03, while the upper part is composed of a horizontally hollow square metal plate and four vertically extending lead screw slide rails C07. The movable support frame is composed of a movable slide rail C05 and an L-shaped metal plate, with a sampling motor fixing hole and a sampling module B fixing hole C15 located at the short end of the L-shape on the right side.

[0067] In use, the up-and-down movement of the lifting bracket C02 is adjusted by controlling the motor meshing with the lifting slide rail C03, thereby driving the sampling module B to move up and down; the left-and-right movement of the moving bracket is adjusted by controlling the motor meshing with the moving slide rail C05, thereby driving the sampling module B to move left and right.

[0068] By controlling the motor at the short end of the L-shaped moving bracket, the cable B06 of sampling module B is adjusted, driving the roller shutter B05 to move and close the opening at the lower end of the acrylic body B01. By controlling the four motors meshing with the lead screw, the operation of the syringe is adjusted to complete the venting work in the preparation stage and the subsequent collection of gas, water, and sediment samples. By controlling the four solenoid valves connected to the syringe and hose, their opening and closing are adjusted to coordinate with the movement of the lead screw to complete the sampling work.

[0069] This invention mainly includes a cultivation module, a sampling module, and a control module. The cultivation module consists of a cultivation tank body and a movable plate structure to adjust the cultivation volume. The sampling module comprises a square acrylic body with elongated openings on the front and back sides of its bottom, and openings on the sides for water, gas, and water collection. A pressure balancing system, including connecting pipes, a balloon, and a solenoid valve, is connected to the top. A roller shutter structure passes through the elongated openings of the square acrylic body to achieve sealed sample collection. The control module mainly consists of a bottom support, a lifting support, a moving support, a syringe, a hose, and centrifuge tubes, and is equipped with a motor for operations such as screw movement and a control box for controlling the motor. This device, through the coordinated operation of these three modules, overcomes the shortcomings of existing technologies that struggle to simultaneously and continuously collect sediment, water, and gas samples during sediment cultivation. It ensures the consistency and stability of the water-sediment ratio in the cultivation system, reducing experimental errors. This invention is of great significance for improving experimental efficiency, reducing experimental errors, and minimizing the workload of researchers.

[0070] Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

Claims

1. A simulation system for the simultaneous collection and precise control of sediment-water-gas sample cultivation processes, characterized in that, It includes a culture module, a sampling module, and a control module; The culture module includes a culture chamber for storing sediments and water, and a culture environment is formed inside the culture chamber. The sampling module includes a sampling body, which is provided with a collection mechanism and multiple through holes for injecting gas or sampling. The collection mechanism includes blocking components corresponding to the multiple through holes. The control module includes a first drive mechanism connected to the blocking components to switch the opening and closing of the through holes through the blocking components. The sampling body is set in the culture environment, and the first driving mechanism controls the shielding component to switch the opening and closing of the through hole for synchronous sampling.

2. The simulation system for simultaneous collection and precise control of sediment-water-gas sample cultivation process according to claim 1, characterized in that, The sampling body has a drainage hole, a water collection hole and a gas collection hole on its side wall. The drainage hole and the water collection hole are located in the lower part of the side wall, and the gas collection hole is located in the upper middle part of the side wall. The sampling body has elongated through holes on its opposite side walls, and two shielding components are correspondingly arranged at the elongated through holes; the bottom of the sampling body has a fully open through hole, and the top has a first through hole for inserting a connecting pipe. One end of the connecting pipe passes through the first through hole and is connected to the first sealing component, while the other end is connected to the solenoid valve.

3. The simulation system for simultaneous collection and precise control of sediment-water-gas sample cultivation process according to claim 2, characterized in that, The first driving mechanism includes pulley groups respectively disposed above two elongated through holes and a first power source assembly, with each pulley group disposed above one of the elongated through holes; The first power source assembly includes a first motor, and the main shaft of the first motor is connected to the transmission rod through a transmission gear; Each pulley assembly includes a pulley bracket on which a pulley is mounted. A cable is wound around the pulley. The pulley is connected to a transmission gear via a transmission rod. One end of the cable is connected to the shielding component, and the other end of the cable is connected to the transmission rod.

4. The simulation system for simultaneous collection and precise control of sediment-water-gas sample cultivation process according to claim 1, characterized in that, The culture module includes a movable plate erected in the culture chamber. The movable plate is connected to the first driving mechanism, which drives the movable plate to move along the length of the culture chamber. After each sampling, the movement of the movable plate maintains the height ratio of water to sediment in the culture chamber.

5. The simulation system for precise control of simultaneous sediment-water-gas sample collection and culture process according to claim 1 or 4, characterized in that, The culture module includes a water circulation system connected to the culture chamber.

6. The simulation system for simultaneous collection and precise control of sediment-water-gas sample cultivation process according to claim 1, characterized in that, The control module includes a support frame and a control system; The support bracket includes a bottom bracket, on which a lifting bracket and a moving bracket are provided for controlling and adjusting the height and horizontal position of the sampling module. The moving bracket is mounted on the lifting bracket, and the sampling module is mounted on the moving bracket.

7. The simulation system for simultaneous collection and precise control of sediment-water-gas sample cultivation process according to claim 1, characterized in that, The bottom support is equipped with multiple syringes for collecting water and gas samples, and the lower part of the bottom support is equipped with multiple slots for storing centrifuge tubes for water samples, turbid water and gas samples, hoses for connecting syringes, centrifuge tubes, gas collection bags and sampling modules, three-way valves for controlling the opening and closing of the passage, motors and lead screws for controlling the operation of syringes.

Images