Bursaphelenchus xylophilus separating device

Abstract

The invention discloses a pine wood nematode separation device which comprises a sample container, a stirrer, a heating pipe, a filter, a separation tank and a collection box, the stirrer is arranged in the sample container; the heating pipe is arranged at the bottom of the sample container, and the top of the heating pipe extends into a stirring shaft of the stirrer; the surface of the stirring shaft is provided with a heating hole for liquid to enter to be in contact with the heating pipe; a water inlet of the filter is communicated with a water outlet of the sample container; a liquid inlet of the separation tank is communicated with a liquid outlet of the filter; and the collecting box is positioned below the liquid outlet of the separating tank. The separation device can improve the separation efficiency.

Description

Technical Field

[0001] This invention relates to the field of pine wood nematode separation technology, and more specifically to a pine wood nematode separation device. Background Technology

[0002] The pine wilt nematode (Bursaphelenchus xylophilus) is an extremely dangerous invasive alien pest that can cause pine wilt disease, which can devastate pine trees, causing them to wither and die in a short period of time, and seriously threatening the pine forest ecosystem and the timber industry.

[0003] Nematodes are arguably the most deadly disease affecting pine trees. Prevention of pine nematode infestation is essential to avoid its damage. Because nematodes are extremely small microorganisms, invisible to the naked eye, specialized instruments are typically required for observation and detection. Furthermore, since nematodes reside inside the pine wood, detection requires first splitting the pine into strips and soaking them in water to separate the nematodes from the wood. Currently, pine nematode separation is achieved by soaking the pine wood in water, which is convenient but time-consuming and not very effective.

[0004] Therefore, developing a pine wood nematode separation device that can improve separation efficiency is a problem that urgently needs to be solved by those skilled in the art. Summary of the Invention

[0005] In view of this, the present invention provides a pine wood nematode separation device that can improve separation efficiency.

[0006] To achieve the above objectives, the present invention adopts the following technical solution:

[0007] A device for separating pine wood nematodes, comprising:

[0008] Sample container,

[0009] A stirrer, which is placed inside the sample container;

[0010] A heating tube is placed at the bottom of the sample container and its top extends into the stirring shaft of the stirrer; the surface of the stirring shaft is provided with heating holes for liquid to enter and contact the heating tube;

[0011] A filter, wherein the inlet of the filter is connected to the outlet of the sample container;

[0012] A separation tank, wherein the inlet of the separation tank is connected to the outlet of the filter;

[0013] A collection box is located below the liquid outlet of the separation tank.

[0014] The beneficial effects of adopting the above technical solution are that the separation speed of pine wood nematodes can be accelerated by using a stirrer and a heating tube, which can effectively improve the separation efficiency. At the same time, the pine wood nematodes can be effectively separated and collected by using a filter, separation tank and collection box, which facilitates subsequent detection and research.

[0015] Preferably, the surface of the stirring shaft is provided with stirring blades, which are arc-shaped; a stirring motor is provided at the top of the stirring shaft, the stirring motor is located outside the sample container and connected to the top of the sample container. The arc-shaped stirring blades can more gently stir the sample, reduce mechanical damage to pine wood nematodes, and improve the separation quality.

[0016] Preferably, the sample container has a top cover, and a lifting assembly is provided on the outside of the sample container. The lifting end of the lifting assembly is connected to the top cover and drives the top cover to move up and down. The design of the top cover and the lifting assembly on the top of the sample container allows the top cover to move up and down easily. This not only facilitates the placement of loose material samples, but also ensures a tight seal between the top cover and the sample container during the separation process, preventing sample liquid leakage and ensuring the stability and safety of the separation process.

[0017] Preferably, the lifting assembly includes: a screw, a lifting gear, a driving gear, a driven gear, and a lifting motor; the bottom of the screw is connected to the outer wall of the sample container, and the top of the screw passes through the top cover and is positioned on top of the top cover; the lifting motor is located on top of the top cover, and the output end of the lifting motor is connected to the driving gear, which is connected to the lifting gear through the driven gear. This structure enables the top cover to be tightly engaged or disengaged from the sample container, improving operational convenience and the reliability of the separation device.

[0018] Preferably, a support plate is provided on the outside of the sample container, and the bottom plate of the screw is connected to the support plate.

[0019] Preferably, a filter screen is provided at the outlet of the sample container. The filter screen at the outlet of the sample container can perform preliminary filtration of loose material or large particulate impurities inside the sample container, preventing large particulate impurities from clogging the delivery pipeline. This helps protect subsequent filters and other components, extends the service life of the equipment, and ensures the smooth operation of the separation process.

[0020] Preferably, a pressure chamber is provided in the pipeline between the sample container and the filter. The pressure chamber is connected to an air compressor for providing pressure, and a pressure gauge and a pressure relief valve are provided in the pressure chamber. The presence of the pressure gauge and pressure relief valve allows for real-time monitoring of the pressure in the pressure chamber and timely pressure relief when the pressure exceeds a set value, ensuring the safe operation of the equipment and further improving separation efficiency and safety.

[0021] Preferably, the filter contains a microfiltration layer and a fine filtration layer. The microfiltration layer removes larger impurity particles, while the fine filtration layer further removes smaller impurities, ensuring that pine wood nematodes can pass through smoothly, while improving the purity and quality of the separation.

[0022] Preferably, a turntable is provided at the bottom of the separation tank, and a rotary motor is connected to the bottom of the turntable; a limit ring is rotatably connected to the side wall of the separation tank. This design helps to achieve efficient separation of pine wood nematodes from the liquid, improving the separation effect and efficiency.

[0023] Preferably, the top of the collection box is open, and an ultrafiltration membrane is disposed inside the collection box. The ultrafiltration membrane has a frame along its edge, and an overlapping rod is connected to the frame, which overlaps the top of the collection box. The ultrafiltration membrane can effectively trap pine wood nematodes while allowing liquid to pass through smoothly. The design of the ultrafiltration membrane allows pine wood nematodes to be collected concentratedly on the membrane, facilitating subsequent detection or collection operations and improving the convenience and accuracy of separation.

[0024] As can be seen from the above technical solution, compared with the prior art, the present invention discloses a pine wood nematode separation device, the beneficial effects of which are:

[0025] (1) In this invention, efficient separation of pine wood nematodes is achieved through components such as sample container, stirrer, heating tube, filter, separation tank and collection box;

[0026] (2) This device not only improves the separation efficiency, but also reduces the damage to pine wilt nematodes through various optimized designs, such as arc-shaped stirring blades, lifting components, pressure chambers, microfiltration layers and fine filtration layers, rotating disks and ultrafiltration membranes, thereby improving the purity and quality of the separation and ensuring the stability and safety of the separation process. It can provide higher quality samples for the detection and research of pine wilt nematodes and help promote the prevention and control of pine wilt disease. Attached Figure Description

[0027] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be briefly introduced below. Obviously, the drawings described below are only embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on the provided drawings without creative effort.

[0028] Figure 1 This is a schematic diagram of the separation device provided by the present invention;

[0029] Figure 2 A side view of the separation device provided by the present invention;

[0030] Figure 3Provided by the present invention Figure 2 Sectional view at point AA.

[0031] In the figure,

[0032] 1-Sample container;

[0033] 11-Top cover; 12-Support plate; 13-Filter screen;

[0034] 2-Agitator;

[0035] 21-Stirring shaft; 22-Heating hole; 23-Stirring blades; 24-Stirring motor;

[0036] 3-Heating element;

[0037] 4-Filter;

[0038] 41-Microfiltration layer; 42-Fine filtration layer;

[0039] 5-Separation tank;

[0040] 51-Turntable; 52-Rotary motor; 53-Limit ring;

[0041] 6-Collection box;

[0042] 61-Ultrafiltration membrane; 62-Overlapping rod;

[0043] 7-Lifting assembly;

[0044] 71-Screw; 72-Lifting gear; 73-Driving gear; 74-Driven gear; 75-Lifting motor;

[0045] 8-Pressure chamber;

[0046] 81-Pressure gauge; 82-Pressure relief valve;

[0047] 9-Air compressor. Detailed Implementation

[0048] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0049] This invention discloses a pine wood nematode separation device, comprising:

[0050] Sample container 1,

[0051] Stirrer 2 is placed inside sample container 1;

[0052] Heating tube 3 is placed at the bottom of sample container 1 and its top extends into the stirring shaft 21 of stirrer 2; the surface of stirring shaft 21 is provided with heating holes 22 for liquid to enter and contact with heating tube 3.

[0053] Filter 4, the inlet of filter 4 is connected to the outlet of sample container 1;

[0054] Separator 5, the inlet of separator 5 is connected to the outlet of filter 4;

[0055] Collection box 6 is located below the liquid outlet of separation tank 5. Stirring and heating can accelerate the separation of pine wood nematodes from pine wood, thereby improving the separation efficiency.

[0056] To further optimize the above technical solution, stirring blades 23 are provided on the surface of the stirring shaft 21, and the stirring blades 23 are arc-shaped; a stirring motor 24 is provided on the top of the stirring shaft 21, and the stirring motor 24 is placed outside the sample container 1 and connected to the top of the sample container 1. The arc-shaped surface of the stirring blades 23 faces the direction of rotation, and the arc shape of the stirring blades 23 can reduce the damage to pine wood nematodes during the rotation of the stirring blades.

[0057] To further optimize the above technical solution, a top cover 11 is attached to the top of the sample container 1, and a lifting assembly 7 is provided on the outside of the sample container 1. The lifting end of the lifting assembly 7 is connected to the top cover 11 and drives the top cover 11 to move up and down. The lifting assembly 7 can drive the top cover 11 to move up and down, which not only makes it convenient to open the top cover 11 to put in the loose material, but also ensures that the top cover 11 and the sample container 1 remain in a sealed and fastened state during the separation process.

[0058] To further optimize the above technical solution, the lifting assembly 7 includes: a screw 71, a lifting gear 72, a driving gear 73, a driven gear 74, and a lifting motor 75. The bottom of the screw 71 is connected to the outer wall of the sample container 1, and the top passes through the top cover 11 and is placed on top of the top cover 11. The lifting motor 75 is located on top of the top cover 11, and the output end of the lifting motor 75 is connected to the driving gear 73. The driving gear 73 is connected to the lifting gear 71 through the driven gear 74. The lifting gear 72, driving gear 73, and driven gear 74 are all located on top of the top cover 11 and are rotatably connected to the top cover 11. The lifting gear 72 rotates under the drive of the driving gear 73 and the driven gear 74. The lifting gear 72 is threadedly connected to the screw 71, so that while the lifting gear 72 rotates, it can drive the top cover 11 to move up and down along the axis of the screw 71, thereby realizing the engagement or disengagement of the top cover 11 and the sample container 1.

[0059] To further optimize the above technical solution, a support plate 12 is provided on the outside of the sample container 1, and the bottom plate of the screw 71 is connected to the support plate 12.

[0060] To further optimize the above technical solution, a filter screen 13 is installed at the outlet of the sample container 1. The filter screen 13 can perform preliminary filtration of loose material or large particulate impurities in the sample container 1, preventing large particulate impurities from clogging the delivery pipeline.

[0061] To further optimize the above technical solution, a pressure chamber 8 is provided on the pipeline between the sample container 1 and the filter 4. An air compressor 9 is connected to the pressure chamber 8 to provide pressure. A pressure gauge 81 and a pressure relief valve 82 are installed in the pressure chamber 8. The air compressor 9 pressurizes the pressure chamber 8. After the sample liquid passes through the pressure chamber 8, the pressure of the sample liquid increases. Appropriate pressurization can improve the filtration speed. When the pressure in the pressure chamber 8 exceeds a set value, the pressure is released through the pressure relief valve 82. A valve is provided between the air compressor 9 and the pressure chamber 8.

[0062] To further optimize the above technical solution, the filter 4 is provided with a microfiltration layer 41 and a fine filtration layer 42. The microfiltration layer 41 has a pore size of 100-200 micrometers and can be made of polyester fiber or nylon fiber; the fine filtration layer 42 has a pore size of 50-100 micrometers and can be made of polytetrafluoroethylene membrane or polyvinylidene fluoride membrane. The diameter of pine wood nematode is about 15-20 micrometers, and the pore size of the microfiltration layer 41 and the fine filtration layer 42 is sufficient for the pine wood nematode to pass through without getting stuck.

[0063] To further optimize the above technical solution, a turntable 51 is provided at the bottom of the separation tank 5, and a rotary motor 52 is connected to the bottom of the turntable 51; a limit ring 53 is rotatably connected to the side wall of the separation tank 5. The turntable 51 drives the separation tank 5 to rotate. Since the density of pine wood nematodes is greater than that of liquid, the pine wood nematodes and liquid will separate into layers under the action of centrifugal force, with the pine wood nematodes in the lower layer and the liquid in the upper layer.

[0064] To further optimize the above technical solution, the top of the collection box 6 is open, and an ultrafiltration membrane 61 is installed inside the collection box 6. A frame is provided around the edge of the ultrafiltration membrane 61, and an overlapping rod 62 is connected to the frame, overlapping the top of the collection box 6. The pore size of the ultrafiltration membrane 61 is generally 0.001-0.02 micrometers, and the material can be polyvinylidene fluoride membrane, polyethersulfone membrane, or polyacrylonitrile membrane. This pore size range can effectively trap pine wood nematodes while allowing liquid to pass through smoothly. The pine wood nematodes are trapped in the ultrafiltration membrane 61. The ultrafiltration membrane 61 can be removed from the collection box 6 for detection or collection of the pine wood nematodes. The overlapping rod facilitates the removal of the ultrafiltration membrane 61 from the collection box 6 for pine wood nematode detection.

[0065] The various embodiments in this specification are described in a progressive manner, with each embodiment focusing on the differences from other embodiments. The same or similar parts between the various embodiments can be referred to each other.

[0066] The above description of the disclosed embodiments enables those skilled in the art to make or use the invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the general principles defined herein may be implemented in other embodiments without departing from the spirit or scope of the invention. Therefore, the invention is not to be limited to the embodiments shown herein, but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims

1. A device for separating pine wood nematodes, characterized in that, include: Sample container, A stirrer, which is placed inside the sample container; A heating tube is placed at the bottom of the sample container and its top extends into the stirring shaft of the stirrer; the surface of the stirring shaft is provided with heating holes for liquid to enter and contact the heating tube; A filter, wherein the inlet of the filter is connected to the outlet of the sample container; A separation tank, wherein the inlet of the separation tank is connected to the outlet of the filter; A collection box is located below the liquid outlet of the separation tank.

2. The pine wood nematode separation device according to claim 1, characterized in that, The surface of the stirring shaft is provided with stirring blades, which are arc-shaped; a stirring motor is provided at the top of the stirring shaft, which is located outside the sample container and connected to the top of the sample container.

3. The pine wood nematode separation device according to claim 1, characterized in that, The sample container is fitted with a top cover, and a lifting assembly is provided on the outside of the sample container. The lifting end of the lifting assembly is connected to the top cover and drives the top cover to move up and down.

4. The pine wood nematode separation device according to claim 3, characterized in that, The lifting assembly includes: a screw, a lifting gear, a driving gear, a driven gear, and a lifting motor; the bottom of the screw is connected to the outer wall of the sample container, and the top passes through the top cover and is placed on top of the top cover; the lifting motor is located on top of the top cover, and the output end of the lifting motor is connected to the driving gear, and the driving gear is connected to the lifting gear through the driven gear.

5. The pine wood nematode separation device according to claim 4, characterized in that, The sample container is provided with a support plate on its exterior, and the bottom plate of the screw is connected to the support plate.

6. The pine wood nematode separation device according to claim 5, characterized in that, A filter screen is installed at the outlet of the sample container.

7. The pine wood nematode separation device according to claim 1, characterized in that, A pressure chamber is provided on the pipeline between the sample container and the filter. The pressure chamber is connected to an air compressor for providing pressure. A pressure gauge and a pressure relief valve are provided at the pressure chamber.

8. The pine wood nematode separation device according to claim 7, characterized in that, The filter contains a microfiltration layer and a fine filtration layer.

9. The pine wood nematode separation device according to claim 1, characterized in that, The bottom of the separation tank is equipped with a turntable, and a rotary motor is connected to the bottom of the turntable; the side wall of the separation tank is rotatably connected with a limit ring.

10. The pine wood nematode separation device according to claim 1, characterized in that, The top of the collection box is open, and an ultrafiltration membrane is installed inside the collection box. The edge of the ultrafiltration membrane is provided with a frame, and the frame is connected to an overlapping rod, which overlaps the top of the collection box.

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