Automatic reagent sample adding device and use method thereof

Abstract

The invention provides an automatic reagent sample adding device and a using method thereof.The automatic reagent sample adding device comprises a sample adding assembly and a cleaning assembly, the sample adding assembly comprises a three-axis portal frame sliding table, a sample adding needle and a sample pipe frame, and the three-axis portal frame sliding table drives the sample adding needle and the sample pipe frame to conduct linear movement sample adding in the sample adding program executing process; the sample adding assembly further comprises a bin frame and a bin cover, and the lower end of the bin cover is detachably connected with a reagent bin used for storing a reagent needing to be added with a sample; the cleaning assembly comprises a cleaning tank for storing cleaning liquid, a shaking mechanism for driving the reagent bin to shake and an air inlet communicated with the bin cover, the cleaning liquid in the cleaning tank is discharged into the reagent bin for shaking cleaning operation in the process of executing the cleaning procedure, and drying gas is introduced through the air inlet for drying after cleaning is completed. The problem of cross contamination caused by the fact that reagents remain in the bin wall, the pipeline and the sample adding needle after sample adding is effectively solved.

Description

TECHNICAL FIELD

[0001] The present application relates to the technical field of biological experiments, and in particular to a reagent automatic dispensing device and a use method thereof. BACKGROUND

[0002] The reagent automatic dispensing device generally comprises a reagent bin, a dispensing needle, a driving mechanism and a control system, and the core function thereof is to transfer the reagent stored in the reagent bin to a test tube or a reaction container through the dispensing needle.

[0003] However, the conventional reagent bin adopts a fixed structure, and after the reagent dispensing is completed, residues are easily left in the bin wall, the pipeline and the dispensing needle, which leads to cross contamination in subsequent use. Although some equipment is equipped with a cleaning function, only the end of the dispensing needle can be flushed, and the inside of the reagent bin and the pipeline cannot be cleaned comprehensively. SUMMARY

[0004] The present application solves the technical problems of the prior art, and provides a reagent automatic dispensing device and a use method thereof.

[0005] To solve the technical problems of the conventional reagent bin adopting a fixed structure, residues being easily left in the bin wall, the pipeline and the dispensing needle after the reagent dispensing is completed, leading to cross contamination in subsequent use, and although some equipment is equipped with a cleaning function, only the end of the dispensing needle can be flushed, and the inside of the reagent bin and the pipeline cannot be cleaned comprehensively, the technical scheme adopted by the present application is as follows: The reagent automatic dispensing device and the use method thereof comprise a dispensing assembly and a cleaning assembly, the dispensing assembly comprises a three-axis gantry sliding table, a dispensing needle and a sample tube rack, and the three-axis gantry sliding table drives the dispensing needle and the sample tube rack to move linearly for dispensing during execution of a dispensing program. The dispensing assembly further comprises a bin rack and a bin cover, and the bin cover is detachably connected with a reagent bin used for storing the reagent to be dispensed. The cleaning assembly comprises a cleaning tank used for storing a cleaning liquid, a shaking mechanism used for shaking the reagent bin, and an air inlet communicated with the bin cover, and the cleaning liquid in the cleaning tank is discharged into the reagent bin for shaking and cleaning during execution of a cleaning program, and dry gas is introduced through the air inlet for drying after the cleaning is completed.

[0006] Preferably, the three-axis gantry sliding table comprises a Y-axis electric sliding table used for longitudinal movement, an X-axis electric sliding table used for transverse movement, and a Z-axis electric sliding table arranged at the front end of the X-axis electric sliding table and used for vertical movement, the dispensing needle is clamped at the front end of the Z-axis electric sliding table, and the sample tube rack is fixed at the upper end of the Y-axis electric sliding table.

[0007] Preferably, the reagent bin is in a bottle shape, a first liquid pump is mounted on the upper end of the bin cover, a liquid suction pipe is fixedly connected to the liquid inlet of the lower end of the first liquid pump, the liquid suction pipe extends downward to the inner bottom of the reagent bin, and the liquid outlet of the first liquid pump is connected to the upper end of the sample adding needle through an electromagnetic valve and a connecting hose.

[0008] Preferably, the cleaning tank is fixedly connected to a second liquid pump at the bottom, a liquid distribution pipe is fixedly connected to the water outlet of the outer end of the second liquid pump, the upper end of the bin cover is fixedly connected to a liquid inlet pipe, and the liquid distribution pipe is connected to the liquid inlet pipe through an electromagnetic valve and a connecting hose.

[0009] Preferably, the shaking mechanism comprises a motor fixedly arranged on the inner side of the upper end of the three-axis gantry sliding table, a transmission gear is fixedly arranged on the motor transmission shaft, a half transmission gear is connected to the upper end of the transmission gear, a rotating shaft column is fixedly arranged on the upper end of the half transmission gear, the rotating shaft column is rotatably connected to the rear side of the upper end of the three-axis gantry sliding table through a rotating shaft, and the bin rack is fixedly arranged on the outer end of the rotating shaft column.

[0010] Preferably, a U-shaped extension rack is fixedly arranged on the upper end of the bin rack, a first arc-shaped block for supporting the reagent bin is fixedly arranged on the inner side of the extension rack, a sliding rod is movably connected to the outer end of the extension rack, and a second arc-shaped block for extruding and supporting the reagent bin is fixedly arranged on the inner side of the sliding rod.

[0011] Preferably, a connecting plate is fixedly arranged on the outer end of the sliding rod, a spring is fixedly arranged on the inner side of the connecting plate, the spring is fixedly arranged on the outer side of the extension rack away from the connecting plate, and the spring is kept in a contracted state.

[0012] Preferably, the air inlet is connected to an external dry gas source through an electromagnetic valve and an air pipe, a waste liquid tank is vertically fixedly arranged on the lower end of the three-axis gantry sliding table, the lower end of the waste liquid tank is in a conical shape, a waste liquid outlet is fixedly arranged on the lower end of the waste liquid tank, and a switch valve is arranged on the outer end of the waste liquid outlet. A reagent automatic sample adding device and a method for using the same, the method comprising the following steps: S1: first, a reagent tube to be added is vertically arranged on the upper end of the sample tube rack, then the three-axis gantry sliding table is controlled to be opened, the sample adding needle is moved to the upper side of the reagent tube together with the sample tube rack, the first liquid pump is controlled to be opened, the reagent in the reagent bin is pumped out through the liquid suction pipe and is discharged into the sample adding needle, after the reagent is discharged into the sample adding needle, the sample adding needle is controlled to be opened, and the inside of the reagent tube is continuously added; S2: after the reagent is added, the second liquid pump is controlled to be opened, the cleaning liquid in the cleaning tank is discharged into the inside of the reagent bin through the liquid distribution pipe, the inside of the reagent bin is cleaned and washed, the cleaning liquid in the reagent bin is discharged into the inside of the sample adding needle through the liquid suction pipe and the first liquid pump, and the cleaning liquid after washing is discharged into the inside of the waste liquid tank through the sample adding needle; S3: During the cleaning process, the motor is controlled to be turned on to drive the transmission gear to move back and forth, the transmission gear is engaged to drive the half transmission gear plate to move back and forth, and then the rotating shaft column and the rack are driven to move back and forth, and the back and forth movement of the rack will synchronously drive the reagent bin to shake and clean; S4: After the flushing and cleaning are completed, the external drying gas source is controlled to be turned on, the drying gas is discharged into the reagent bin through the air inlet, and the drying gas will flow to the sample adding needle in sequence and then be discharged outward, so that the reagent bin, the reagent conveying pipeline and the sample adding needle are continuously dried.

[0013] Compared with the prior art, the beneficial effects of the present application are: After the reagent sample adding is completed, the second liquid pump is controlled to be turned on to cooperate with the liquid distribution pipe to discharge the cleaning liquid in the cleaning tank into the reagent bin for flushing and cleaning, and at the same time, the first liquid pump cooperates with the liquid suction pipe to discharge the cleaning liquid in the reagent bin into the sample adding needle, and then the flushing cleaning liquid is discharged into the waste liquid tank through the sample adding needle, so that the cleaning liquid continuously flushes and cleans the reagent bin, the reagent conveying pipeline and the sample adding needle, and effectively avoids the problem of cross contamination caused by the residue of the reagent in the bin wall, the pipeline and the sample adding needle after the sample adding; After the flushing and cleaning are completed, the external drying gas source is controlled to be turned on, the drying gas is discharged into the reagent bin through the air inlet, and the drying gas will flow to the sample adding needle in sequence and then be discharged outward, so that the reagent bin, the reagent conveying pipeline and the sample adding needle are continuously dried. BRIEF DESCRIPTION OF DRAWINGS

[0014] The disclosure of the present application will be described with reference to the accompanying drawings. It should be understood that the drawings are only for illustrative purposes, and are not intended to limit the scope of protection of the present application. In the drawings, the same reference numerals are used to refer to the same parts. Among them: Figure 1 is a schematic diagram of the overall structure of the present application Figure 1 ; Figure 2 is a schematic diagram of the overall structure of the present application Figure 2 ; Figure 3 is a schematic diagram of the overall structure of the present application Figure 4 is a schematic diagram of the overall structure of the present application Figure 5 is a schematic diagram of the overall structure of the present application

[0015] 101, three-axis gantry sliding table; 1011, Y-axis electric sliding table; 1012, X-axis electric sliding table; 1013, Z-axis electric sliding table; 102, sample adding needle; 103, sample tube rack; 104, rack cover; 105, rack cover; 106, reagent rack; 107, first liquid pump; 108, liquid suction pipe; 109, extension rack; 110, first arc-shaped block; 111, sliding rod; 112, second arc-shaped block; 113, connecting plate; 114, spring; 201, cleaning tank; 2011, liquid inlet; 202, shaking mechanism; 2021, motor; 2022, transmission gear; 2023, half transmission gear disc; 2024, rotating shaft column; 203, second liquid pump; 204, liquid distribution pipe; 205, liquid inlet pipe; 206, waste liquid tank; 207, waste liquid inlet; 208, gas inlet. DETAILED DESCRIPTION

[0016] It is easy to understand that, according to the technical solution of the present application, those skilled in the art can propose various structural modes and implementation modes that can be replaced with each other without changing the essential spirit of the present application. Therefore, the following detailed description and the accompanying drawings are only exemplary descriptions of the technical solution of the present application, and should not be regarded as the whole or as a limitation or restriction on the technical solution of the present application.

[0017] The specific embodiments of the present application will be described below in conjunction with the accompanying drawings.

[0018] Please refer to Figure 1 - Figure 3 The present embodiment proposes an automatic reagent sample adding device and its use method, which integrates a sample adding assembly and a cleaning assembly.

[0019] In terms of the sample adding assembly, its main structure is constructed on the basis of a three-axis gantry sliding table 101. A sample adding needle 102 is arranged on the sliding table part at the upper end of the three-axis gantry sliding table 101 by clamping, and a sample tube rack 103 is fixedly installed on the sliding table part at the lower end of the three-axis gantry sliding table 101. In particular, the sample adding needle 102 is equipped with a liquid level sensor (not explicitly shown in the figure) for real-time monitoring of the internal liquid level. By accurately controlling the three-axis gantry sliding table 101, the sample adding needle 102 and the sample tube rack 103 can be driven to move linearly, achieving accurate sample adding.

[0020] Further, the structure of the three-axis gantry sliding table 101 comprises a longitudinally moving Y-axis electric sliding table 1011 at the lower end, a transversely moving X-axis electric sliding table 1012 at the upper end, and a vertically moving Z-axis electric sliding table 1013 arranged at the front end of the X-axis electric sliding table 1012. The sample adding needle 102 is vertically clamped at the front end of the Z-axis electric sliding table 1013 by buckling, and the sample tube rack 103 is horizontally fixed at the upper end of the Y-axis electric sliding table 1011 by screws. Such design enables the X-axis electric sliding table 1012 and the Z-axis electric sliding table 1013 to work cooperatively to drive the sample adding needle 102 to move linearly vertically or transversely; at the same time, the Y-axis electric sliding table 1011 is responsible for driving the sample tube rack 103 to move linearly longitudinally.

[0021] Please continue to see Figure 3 - Figure 5 The sample adding assembly further comprises a rack 104 and a plurality of rack covers 105 embedded through the upper end of the rack 104, which are arranged in sequence at the upper end of the rack 104. The lower end of each rack cover 105 is detachably connected with a reagent tank 106 by threads, and the reagent tank 106 is in the form of a bottle structure for quick disassembly and replacement. The upper end of the rack cover 105 is provided with a first liquid pump 107, and the lower end thereof is fixedly connected with a suction pipe 108 at the liquid inlet, which extends downward to the inner bottom of the reagent tank 106. The liquid outlet of the first liquid pump 107 is connected in communication with the upper end of the sample adding needle 102 through an electromagnetic valve and a connecting hose (not explicitly shown in the figure), so as to ensure that the first liquid pump 107 can cooperate with the suction pipe 108 to suck out the reagent in the reagent tank 106 and discharge it into the sample adding needle 102.

[0022] The cleaning assembly comprises a cleaning tank 201 supported and fixed on the upper end of the rear side of the three-axis gantry sliding table 101 by bolts, and a shaking mechanism 202 for shaking the reagent tank 106. The upper end of the cleaning tank 201 is provided with a liquid inlet 2011 for injecting cleaning liquid or pure water. The bottom of the cleaning tank 201 is fixedly connected with a second liquid pump 203, and the outer end of the water outlet of the second liquid pump 203 is fixedly connected with a distribution pipe 204. The upper end of the rack cover 105 is fixedly connected with a liquid inlet pipe 205, and the distribution pipe 204 is connected in communication with the liquid inlet pipe 205 through an electromagnetic valve and a connecting hose (not explicitly shown in the figure), so as to ensure that the second liquid pump 203 can cooperate with the distribution pipe 204 to discharge the cleaning liquid or pure water in the cleaning tank 201 along the rack cover 105 into the reagent tank 106.

[0023] The shaking mechanism 202 includes a motor 2021 fixed on the inner side of the upper end of the three-axis gantry sliding table 101 by screws, and the rear end transmission shaft of the motor 2021 is connected to the rear side of the upper end of the three-axis gantry sliding table 101 through a rotating shaft. The transmission shaft of the motor 2021 is partially fixedly installed with a transmission gear 2022, and the upper end of the transmission gear 2022 is meshingly connected with a half transmission gear disc 2023. The upper end of the half transmission gear disc 2023 is fixedly installed with a rotating shaft column 2024, and the rotating shaft column 2024 is rotatably connected to the rear side of the upper end of the three-axis gantry sliding table 101 through a rotating shaft. The rack 104 is welded and fixed to the outer end of the rotating shaft column 2024, and the rack 104 is driven to swing by the reciprocating movement of the rotating shaft column 2024, thereby synchronously driving the reagent rack 106 to shake and clean.

[0024] Please continue to read Figure 4 - Figure 5 The upper end of the rack 104 is fixedly installed with a U-shaped extension frame 109, and the inner side of the extension frame 109 is adhesively fixed with a first arc-shaped block 110 for supporting the reagent rack 106. The outer end of the extension frame 109 is rotatably connected with a slide rod 111, and the inner side of the slide rod 111 is adhesively fixed with a second arc-shaped block 112 for extruding and supporting the reagent rack 106. The outer end of the slide rod 111 is welded or integrally formed with a connecting plate 113, and the inner side of the connecting plate 113 is fixedly installed with a spring 114. The spring 114 is fixedly installed on the outer side of the extension frame 109 away from the connecting plate 113, and remains in a contracted state. By the elastic force of the spring 114, the connecting plate 113 keeps pushing the slide rod 111 inward, so that the second arc-shaped block 112 continuously extrudes and supports the reagent rack 106, ensuring that the reagent rack 106 remains stable at the lower end of the cover 105.

[0025] In addition, the present embodiment also includes an air inlet 208 fixedly connected to the upper end of the cover 105, which is connected in communication with an external dry gas source through an electromagnetic valve and an air pipe (not explicitly shown in the figure), for circulating and drying the reagent rack 106, the reagent delivery pipeline and the sample adding needle 102.

[0026] Please continue to read Figure 1 - Figure 3 The lower end of the three-axis gantry sliding table 101 is also vertically installed with a waste liquid tank 206 through screws, and the lower end of the waste liquid tank 206 is conical in shape, which is used to collect the cleaning waste liquid discharged from the sample adding needle 102. The lower end of the waste liquid tank 206 is fixedly connected with a waste liquid outlet 207, and the outer end of the waste liquid outlet 207 is installed with an on-off valve, which is convenient for centralized discharge of the cleaning waste liquid collected in the waste liquid tank 206.

[0027] The device is used in actual use, first, the required reagent tube is vertically placed on the upper end of the sample tube rack 103. Then, by controlling the opening of the three-axis gantry sliding table 101, the sample needle 102 is moved to the upper side of the reagent tube. At this time, the first liquid pump 107 is controlled to open, and the reagent in the reagent bin 106 is pumped out and discharged into the sample needle 102. After the reagent is discharged into the sample needle 102, the sample needle 102 is controlled to open, and the inside of the reagent tube can be continuously sampled.

[0028] After the reagent sampling is completed, the second liquid pump 203 is controlled to open, and the cleaning liquid or pure water in the cleaning tank 201 is discharged into the reagent bin 106 for cleaning. At the same time, the first liquid pump 107 cooperates with the liquid pump 108 to discharge the cleaning liquid or pure water in the reagent bin 106 into the sample needle 102, and then the cleaning liquid or pure water is discharged into the waste tank 206 through the sample needle 102. During this process, the cleaning liquid or pure water continuously cleans the reagent bin 106, the reagent conveying pipeline and the sample needle 102, effectively avoiding the problem of cross contamination caused by residual reagent in the bin wall, pipeline and sample needle after sampling.

[0029] During the cleaning process, the motor 2021 can also be controlled to open, driving the transmission gear 2022 to reciprocate. The transmission gear 2022 drives the half transmission gear disc 2023 to reciprocate, and then drives the rotating shaft column 2024 and the bin rack 104 to reciprocate. The reciprocating motion of the bin rack 104 will synchronously drive the reagent bin 106 to shake and clean, so that the cleaning liquid or pure water in the reagent bin 106 can clean the inner wall more thoroughly.

[0030] After the cleaning and cleaning are completed, the external dry gas source is controlled to open, and the dry gas is discharged into the reagent bin 106 through the gas inlet 208. The dry gas will flow to the sample needle 102 and then be discharged outward, continuously flowing through the reagent bin 106, the reagent conveying pipeline and the sample needle 102. This step ensures that the reagent bin 106, the reagent conveying pipeline and the sample needle 102 are continuously cleaned and dried, avoiding the residual situation during the cleaning process.

[0031] The technical scope of the present application is not limited to the above description, and those skilled in the art can make various modifications and modifications to the above embodiments without departing from the technical idea of the present application, and these modifications and modifications should be within the protection scope of the present application.

Claims

1. A reagent automatic sample adding device characterized by comprising: The sample adding assembly and the cleaning assembly, the sample adding assembly comprises a three-axis gantry sliding table, a sample adding needle and a sample tube rack, the three-axis gantry sliding table drives the sample adding needle and the sample tube rack to move linearly for sample adding during the execution of a sample adding program; The sample adding assembly further comprises a rack and a cover, the lower end of the cover is detachably connected with a reagent rack for storing the reagent required for sample adding; The cleaning assembly comprises a cleaning tank for storing cleaning liquid, a shaking mechanism for shaking the reagent rack and an air inlet communicated with the cover, the cleaning liquid in the cleaning tank is discharged into the reagent rack for shaking and cleaning during the execution of a cleaning program, and dry gas is introduced through the air inlet for drying after the cleaning is completed.

2. The reagent automatic sample adding device according to claim 1, characterized in that, The three-axis gantry sliding table comprises a Y-axis electric sliding table moving longitudinally, an X-axis electric sliding table moving transversely and a Z-axis electric sliding table vertically moving at the front end of the X-axis electric sliding table, the sample adding needle is clamped at the front end of the Z-axis electric sliding table, and the sample tube rack is fixed at the upper end of the Y-axis electric sliding table.

3. The reagent automatic sample adding device according to claim 2, characterized in that, The reagent rack has a bottle-like structure, a first liquid pump is mounted at the upper end of the cover, a suction tube is fixedly connected at the liquid inlet at the lower end of the first liquid pump, the suction tube extends downward to the inner bottom of the reagent rack, and the liquid outlet of the first liquid pump is connected in communication with the upper end of the sample adding needle through an electromagnetic valve and a connecting hose.

4. The reagent automatic sample adding device according to claim 3, characterized in that, The lower end of the second liquid pump is fixedly connected with a distribution pipe, the upper end of the cover is fixedly connected with a liquid inlet pipe, and the distribution pipe is connected in communication with the liquid inlet pipe through an electromagnetic valve and a connecting hose.

5. The reagent automatic sample adding device according to claim 4, characterized in that, The shaking mechanism comprises a motor fixed at the inner side of the upper end of the three-axis gantry sliding table, a transmission gear is fixedly mounted on the transmission shaft of the motor, a half transmission gear is engagedly connected at the upper end of the transmission gear, a rotating shaft column is fixedly installed at the upper end of the half transmission gear, the rotating shaft column is rotatably connected to the rear side of the upper end of the three-axis gantry sliding table through a rotating shaft, and the rack is fixed at the outer end of the rotating shaft column.

6. The reagent automatic sample adding device according to claim 5, characterized in that, A U-shaped extension frame is fixedly installed at the upper end of the rack, a first arc-shaped block for supporting the reagent rack is fixed at the inner side of the extension frame, a slide rod is movably connected through the extension frame at the outer end of the extension frame, and a second arc-shaped block for extruding and supporting the reagent rack is fixed at the inner side of the slide rod.

7. The reagent automatic sample adding device according to claim 6, characterized in that, A connecting plate is fixed at the outer end of the slide rod, a spring is fixedly installed at the inner side of the connecting plate, the spring is fixedly installed at the outer side of the extension frame away from the connecting plate, and the spring is kept in a contracted state.

8. The reagent automatic sample adding device according to claim 1, characterized in that, The air inlet is connected in communication with an external dry gas source through an electromagnetic valve and an air pipe, a waste liquid tank is vertically fixed at the lower end of the three-axis gantry sliding table, the lower end of the waste liquid tank is conical, a waste liquid outlet is fixedly connected at the lower end of the waste liquid tank, and a switch valve is mounted at the outer end of the waste liquid outlet.

9. The method of using the reagent automatic spotting device according to any one of claims 1-8, wherein, The use steps are as follows: S1: first, the reagent tube required for sample adding is vertically placed at the upper end of the sample tube rack, then the three-axis gantry sliding table is controlled to be turned on to drive the sample adding needle to move to the upper side of the reagent tube together with the sample tube rack, at this time, the first liquid pump is controlled to be turned on to cooperate with the suction tube to discharge the reagent in the reagent rack and discharge it into the sample adding needle, after the reagent is discharged into the sample adding needle, the sample adding needle is controlled to be turned on to continuously add the reagent in the reagent tube; S2: After the reagent is added, the second liquid pump is controlled to be turned on, and the cleaning liquid or pure water in the cleaning tank is discharged into the reagent bin for cleaning through the liquid distribution pipe. At the same time, the first liquid pump is controlled to be turned on, and the cleaning liquid or pure water in the reagent bin is discharged into the sample needle, and then the cleaning liquid or pure water is discharged into the waste liquid tank through the sample needle; S3: During the cleaning process, the motor is controlled to be turned on to drive the transmission gear to reciprocate, the transmission gear is engaged to drive the half transmission gear to reciprocate, and then the rotating shaft column and the bin frame are driven to reciprocate, and the reciprocation of the bin frame will synchronously drive the reagent bin to shake and clean; S4: After the cleaning and cleaning are completed, the external dry gas source is controlled to be turned on, and the dry gas is discharged into the reagent bin through the gas inlet. The dry gas will flow to the sample needle in turn and be discharged outward, and the reagent bin, the reagent conveying pipeline and the sample needle are continuously dried.

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