A capsule with a function of relieving physical fatigue and a preparation method and application thereof

Abstract

The present application relates to the technical field of biological medicine, in particular to a capsule with the function of relieving physical fatigue, a preparation method and application thereof, the composition contains coenzyme Q10 and mangosteen shell extract as core active ingredients in a specific mass ratio, and preferably, sea buckthorn seed extract is added. The present application further provides two optimized extraction processes of the mangosteen shell extract and the sea buckthorn seed extract. In addition, the composition is further mixed with oily bases, suspending agents, emulsifying agents and other excipients to form a soft capsule dosage form. Through the synergistic effect of various active ingredients, the product can effectively improve the body's endurance, accelerate the removal of metabolic products and enhance the antioxidant capacity, thereby significantly relieving physical fatigue. The product has the characteristics of naturalness, safety, high efficiency, high bioavailability and the like, and can be used for preparing food, health food or medicine for relieving physical fatigue.

Description

Technical Field

[0001] This invention relates to the field of biomedical technology, and in particular to a capsule with the function of relieving physical fatigue, its preparation method and application. Background Technology

[0002] Physical fatigue is a common physiological state, usually caused by excessive physical or mental activity, manifested as energy depletion, accumulation of metabolic waste products, increased oxidative stress, and decreased bodily function. In modern society, the fast pace of life, work pressure, and unhealthy lifestyle habits have led to an increasing number of people experiencing physical fatigue. Long-term fatigue may further lead to decreased immunity, endocrine disorders, and chronic diseases, seriously affecting quality of life and work efficiency.

[0003] Methods to alleviate physical fatigue mainly include rest, nutritional supplementation, and drug intervention. Common anti-fatigue products on the market include energy drinks, vitamin and mineral supplements, and traditional Chinese medicine preparations. However, many products have limitations: first, their ingredients are singular, such as relying solely on caffeine or vitamins, resulting in short-lived effects and a high risk of dependence; second, synthetic drugs may be accompanied by side effects, such as nervous system stimulation or burden on the liver and kidneys; and third, traditional Chinese medicine has a slow onset of action, poor taste, and low user compliance. Therefore, developing a natural, safe, effective, and scientifically formulated anti-fatigue composition has become a research hotspot.

[0004] Mitochondria, as the cell's energy factories, are closely related to fatigue. Coenzyme Q10, a fat-soluble quinone compound, is widely distributed in the inner mitochondrial membrane, participating in electron transport and ATP synthesis, and is a key coenzyme in cellular energy metabolism. Simultaneously, coenzyme Q10 possesses strong antioxidant capabilities, scavenging free radicals and reducing oxidative damage. Studies have shown that exogenous coenzyme Q10 supplementation can significantly improve physical endurance and anti-fatigue ability. However, coenzyme Q10 has low bioavailability when used alone, and its anti-fatigue mechanism is relatively simple, limiting its application effectiveness. Summary of the Invention

[0005] To address the aforementioned technical problems, the present invention aims to provide a capsule with the function of relieving physical fatigue, its preparation method, and its application. The composition contains coenzyme Q10 and mangosteen peel extract in a specific mass ratio as core active ingredients, and sea buckthorn seed extract may preferably be added. This composition can effectively improve physical endurance, accelerate the clearance of metabolic products, and enhance antioxidant capacity, thereby significantly relieving physical fatigue. It has the characteristics of being natural, safe, efficient, and having high bioavailability, and can be used to prepare foods, health foods, or drugs that relieve physical fatigue.

[0006] To achieve the above-mentioned technical effects, the present invention adopts the following technical solution:

[0007] In a first aspect, the present invention aims to provide a composition having the function of relieving physical fatigue, comprising, by weight parts: 5-15 parts of coenzyme Q10 and 10-40 parts of mangosteen fruit shell extract.

[0008] As a preferred technical solution, the mangosteen fruit shell extract is prepared according to the following method:

[0009] A1: Crushing: Crush the dried mangosteen shells and pass them through a 30-80 mesh sieve;

[0010] A2: Extraction: Weigh the mangosteen fruit shell powder and add 70% ethanol solution at a mass-volume ratio of 1:8 - 1:30. Use an ultrasonic-microwave synergistic extractor at a power of 200-300W and a temperature of 50-60℃ for 20-30 minutes.

[0011] A3: Separation: Centrifuge the extract at 4000-4500 r / min for 15 minutes, take the supernatant and filter it, and collect the filtrate;

[0012] A4: Concentration and drying: The filtrate is concentrated under reduced pressure at 50℃-60℃ to recover ethanol, and further concentrated to an extract. The extract is then spray-dried, with the inlet air temperature controlled at 170-185℃ and the outlet air temperature at 75-85℃. After spray drying, the mangosteen shell extract is obtained.

[0013] As a preferred technical solution, the mangosteen fruit shell extract is prepared according to the following method:

[0014] B1: Crushing: Crush the dried mangosteen shells and pass them through a 30-80 mesh sieve;

[0015] B2: Extraction: Weigh the mangosteen fruit shell powder, place it in a round-bottom flask, add 70% ethanol solution at a mass-volume ratio of 1:8 - 1:25, install a hot reflux device, and heat and reflux at 75-85℃ for 1-2 hours.

[0016] B3: Repeated extraction: Filter out the first extract, add the same volume of 70% ethanol solution to the residue, and repeat the extraction 1-2 times under the above conditions, separating the extract after each extraction;

[0017] B4: Concentration and Drying: Combine all extracts, concentrate and recover ethanol under reduced pressure at 50℃-60℃, and concentrate to extract. Spray dry the extract, controlling the inlet air temperature at 170-185℃ and the outlet air temperature at 75-85℃. After spray drying, the mangosteen peel extract is obtained.

[0018] Secondly, based on the technology provided in the first aspect, the present invention further provides a composition having the function of relieving physical fatigue, which, by weight, further includes 15-35 parts of sea buckthorn seed extract.

[0019] As a preferred technical solution, the sea buckthorn seed extract is prepared according to the following method:

[0020] C1: Pretreatment: After crushing the sea buckthorn seeds, pass them through a 40-80 mesh sieve, defatted and dried to obtain pretreated sea buckthorn seed powder;

[0021] C2: Extraction: Add the pretreated sea buckthorn seed powder to a 60%-80% ethanol solution at a material-to-liquid ratio of 1:10 - 1:30, and extract for 20-40 minutes at an ultrasonic power of 300-500W and a temperature of 40-60℃.

[0022] C3: Post-processing: The obtained extract is subjected to solid-liquid separation, concentration and drying to obtain the sea buckthorn seed extract.

[0023] Thirdly, the present invention further provides the use of the composition provided in the first or second aspect above in the preparation of food, health food or medicine for relieving physical fatigue.

[0024] Fourthly, the present invention provides a soft capsule comprising the composition provided in the first or second aspect above, and pharmaceutically or food-grade excipients.

[0025] As a preferred technical solution, the soft capsule includes a capsule shell and contents, the contents being made from the following raw materials in parts by weight:

[0026] The composition provided in the first or second aspect above comprises 50 to 80 parts, an oily matrix of 20 to 50 parts, a suspending agent of 1 to 3 parts, and an emulsifier of 0.5 to 2 parts.

[0027] As a preferred technical solution, the oily matrix is ​​one or more of medium-chain triglycerides, soybean oil, and corn oil; the suspending agent is beeswax or hydrogenated vegetable oil; and the emulsifier is soybean lecithin.

[0028] Fifthly, the present invention also provides a method for preparing the above-mentioned soft capsules, comprising the following steps:

[0029] S1: Preparation of contents: Heat the oily matrix, add a suspending agent to dissolve it, cool it and add an emulsifier, then add the composition described in any one of the first or second aspects above, and after homogenization and degassing, obtain a contents suspension;

[0030] S2: Preparation of capsule shell solution: Mix gelatin, plasticizer, opacifier and water, heat to dissolve, degas to obtain the glue solution;

[0031] S3: Squash compression and drying: The contents suspension and gel are compressed into shape using a soft capsule squashing machine, and then shaped, washed and dried to obtain the soft capsules.

[0032] As a preferred technical solution, the aforementioned S1 includes the following steps:

[0033] (a) Preparation of oil phase: Weigh the oily matrix according to the formula amount and heat it to 65~75℃. Slowly add the suspending agent according to the formula amount while stirring at 300~500 rpm, continue stirring and keep warm for 15~30 minutes until the suspending agent is completely dissolved and a clear and transparent oil phase is formed.

[0034] (b) Cooling and emulsification: Cool the oil phase to below 40°C. While stirring continuously, slowly add the formulated amount of emulsifier and stir for 10 to 20 minutes until it is completely dispersed and uniform to obtain the primary emulsion.

[0035] (c) Solid phase addition and dispersion: Under continuous stirring, the composition provided in the first aspect or the second aspect is slowly and in batches added to the above primary emulsion. After the addition is completed, the stirring speed is increased to 800~1200 rpm and stirred for 20~30 minutes to fully wet the powder and initially disperse it to obtain a coarse suspension.

[0036] (d) Homogenization: Transfer the coarse suspension into a high-shear homogenizer and homogenize at 3000~5000 rpm for 10~20 minutes until a uniform, fine suspension without obvious particles is formed.

[0037] (e) Degassing: Transfer the homogenized suspension into a vacuum degassing tank and degas it for 15 to 30 minutes under a vacuum of -0.08 to -0.10 MPa until no obvious bubbles are precipitated in the suspension, thus obtaining the contents suspension.

[0038] Compared with the prior art, the beneficial effects of the present invention are as follows:

[0039] This invention achieves a significant synergistic effect through the scientific formulation of coenzyme Q10, mangosteen peel extract, and sea buckthorn seed extract. Its anti-fatigue and antioxidant effects are far superior to those of any single component used alone, effectively prolonging exercise endurance, reducing blood lactate and serum urea nitrogen levels, and increasing liver glycogen reserves. Simultaneously, the optimized extraction processes, such as ultrasonic-microwave synergistic extraction, enable more efficient extraction of active ingredients from mangosteen peel, thereby enhancing the final efficacy of the product. Furthermore, the designed soft capsule dosage form cleverly combines fat-soluble active ingredients with an oily matrix, significantly improving absorption efficiency and bioavailability, effectively masking unpleasant odors, and enhancing the user experience. In addition, the product's main components are derived from natural extracts and nutrients, avoiding the potential side effects of chemically synthesized drugs, and offering significant advantages in terms of high safety and good user compliance. Detailed Implementation

[0040] The following embodiments are only used to illustrate the technical solutions of the present invention more clearly, and are therefore only examples and should not be used to limit the scope of protection of the present invention.

[0041] Those skilled in the art will understand that the present invention can be practiced even without certain specific details. In some other embodiments, methods, means, apparatus, and steps well known to those skilled in the art have not been described in detail in order to highlight the spirit of the invention. Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by those skilled in the art. Unless otherwise specified, all units used in this specification are International Standard Units (SI), and all numerical values ​​and ranges appearing in this invention should be understood to include systematic errors unavoidable in industrial production.

[0042] Unless otherwise specified, the experimental methods used in the following examples are all conventional methods; the materials, reagents, or instruments used, unless otherwise specified by the manufacturer, are all commercially available; and the conditions not specified in the examples are all performed under conventional conditions or conditions recommended by the manufacturer. Furthermore, this invention does not limit the source of the raw materials used; unless otherwise specified, the raw materials used in this invention are all commercially available products commonly found in this technical field. Unless otherwise specified, the "ratio" referred to in the following examples refers to a ratio of parts by mass.

[0043] Example 1

[0044] This embodiment provides a composition A1 with the function of relieving physical fatigue. By weight, the raw materials are: 10 parts coenzyme Q10 and 40 parts mangosteen peel extract. The preparation method of the mangosteen peel extract is as follows:

[0045] A1: Grinding: Take the dried mangosteen shells, grind them with a universal grinder, pass them through a 50-mesh standard sieve, and collect the powder that passes through the sieve;

[0046] A2: Extraction: Weigh 100g of the above mangosteen fruit shell powder, add 1500mL of 70% ethanol (70% is volume fraction) solution at a material-liquid ratio of 1:15 (mass-volume ratio, g:mL), transfer to an ultrasonic-microwave co-extraction instrument, set the power to 250W and the extraction temperature to 55℃, and extract continuously for 25 minutes.

[0047] A3: Separation: After extraction, transfer the extract to a high-speed centrifuge, set the speed to 4200 r / min, centrifuge for 15 minutes, take the supernatant and filter it through a 0.45μm microporous membrane, and collect the clear filtrate;

[0048] A4: Concentration and Drying: Transfer the clarified filtrate to a rotary evaporator, set the temperature to 55℃ and the vacuum degree to -0.09MPa, concentrate and recover ethanol under reduced pressure until the concentrate is a thick paste (relative density 1.250, measured at 55℃); send the paste into a spray dryer, set the inlet air temperature to 180℃, the outlet air temperature to 80℃, and the feed rate to 15mL / min, collect the powder after spray drying to obtain mangosteen shell extract.

[0049] Example 2

[0050] This embodiment provides a composition A2 with the function of relieving physical fatigue. By weight, the raw materials are: 10 parts coenzyme Q10 and 40 parts mangosteen peel extract. The preparation method of the mangosteen peel extract is as follows:

[0051] B1: Grinding: Take dried mangosteen shells, grind them with a universal grinder, pass them through a 60-mesh standard sieve, and collect the powder that passes through the sieve;

[0052] B2: Extraction: Weigh 100g of the above mangosteen fruit shell powder and place it in a 2000mL round-bottom flask. Add 1200mL of 70% ethanol solution at a material-to-liquid ratio of 1:12 (mass-volume ratio, g:mL). Install a spherical condenser to form a hot reflux device, set the oil bath temperature to 80℃, and heat and reflux for 1.5 hours.

[0053] B3: Repeated extraction: After the first extraction, filter and collect the extract while it is still hot; add 1200 mL of 70% ethanol solution to the residue and repeat the extraction once under the above conditions of 80℃ and hot reflux, and filter and collect the second extract.

[0054] B4: Concentration and Drying: Combine the two extracts and transfer them to a rotary evaporator. Set the temperature to 55℃ and the vacuum degree to -0.09MPa. Concentrate under reduced pressure to recover ethanol to a thick paste (relative density 1.252, measured at 55℃). Send the paste to a spray dryer. Set the inlet air temperature to 175℃, the outlet air temperature to 80℃, and the feed rate to 12mL / min. After spray drying, collect the powder to obtain the mangosteen shell extract.

[0055] Example 3

[0056] This embodiment provides a composition A3 with the function of relieving physical fatigue. By weight, the raw materials are: 10 parts coenzyme Q10, 20 parts mangosteen fruit shell extract, and 20 parts sea buckthorn seed extract. The mangosteen fruit shell extract is prepared using the method described in Example 1.

[0057] The preparation method of sea buckthorn seed extract is as follows:

[0058] C1: Pretreatment: Take dried sea buckthorn seeds, crush them with a universal pulverizer, and pass them through a 60-mesh standard sieve; weigh 100g of the sieved powder, add 500mL of n-hexane at a material-to-liquid ratio of 1:5 (mass-volume ratio, g:mL), soak at room temperature for 2 hours to degrease, filter to remove n-hexane, and place the filter residue in a 60℃ vacuum drying oven (vacuum degree -0.08MPa) to dry for 2 hours to obtain pretreated sea buckthorn seed powder;

[0059] C2: Extraction: Weigh 50g of pretreated sea buckthorn seed powder, add 1000mL of 70% ethanol solution at a material-to-liquid ratio of 1:20 (mass-volume ratio, g:mL), transfer to an ultrasonic extractor, set the ultrasonic power to 400W and the extraction temperature to 50℃, and extract continuously for 30 minutes.

[0060] C3: Post-processing: After extraction, the extract was transferred to a high-speed centrifuge and centrifuged at 3500 r / min for 10 minutes. The supernatant was then filtered through a 0.45 μm microporous membrane. The clarified filtrate was transferred to a rotary evaporator and concentrated under reduced pressure at 50℃ and -0.09 MPa to a thick paste (relative density 1.21, measured at 55℃). The paste was then dried in a vacuum drying oven at 60℃ (vacuum degree -0.08 MPa) for 4 hours. After pulverizing, the paste was passed through an 80-mesh sieve to obtain the sea buckthorn seed extract.

[0061] Example 4

[0062] This embodiment provides a soft capsule product based on composition A1 described in Example 1 and its preparation method.

[0063] 1. Raw materials and formula

[0064] (1) Contents formulation:

[0065] Composition A1 (10 parts coenzyme Q10 + 40 parts mangosteen peel extract): 50.0 g;

[0066] Oily matrix (soybean oil): 240.0 g;

[0067] Suspension agent (beeswax): 9.0 g;

[0068] Emulsifier (soybean lecithin): 1.0 g;

[0069] Total weight of contents: 300.0 g;

[0070] (2) Capsule shell liquid formulation:

[0071] Gelatin: 100.0 g;

[0072] Plasticizer (glycerin): 50.0 g;

[0073] Light-blocking agent (titanium dioxide): 2.0 g;

[0074] Purified water: 100.0 g;

[0075] 2. The preparation method of the soft capsules provided in this embodiment is as follows:

[0076] S1: Prepare a suspension of the contents

[0077] (a) Oil phase preparation: Weigh soybean oil according to the formula amount and heat it to 70°C. While stirring at 400 rpm, slowly add the beeswax according to the formula amount, continue stirring and keep warm for 20 minutes until the beeswax is completely dissolved and a clear, transparent oil phase is formed.

[0078] (b) Cooling and emulsification: Cool the above oil phase to 35°C. While stirring continuously, slowly add the prescribed amount of soybean lecithin and stir for 15 minutes until it is completely dispersed and uniform to obtain the primary emulsion.

[0079] (c) Solid phase addition and dispersion: Under continuous stirring, 50.0 g of composition A1 was slowly and in batches added to the above primary emulsion. After the addition was complete, the stirring speed was increased to 1000 rpm and stirred for 25 minutes to fully wet and initially disperse the powder, resulting in a coarse suspension.

[0080] (d) Homogenization: Transfer the coarse suspension into a high-shear homogenizer and homogenize at 4000 rpm for 15 minutes until a uniform, fine suspension without obvious particles is formed.

[0081] (e) Degassing: The homogenized suspension is transferred to a vacuum degassing tank and degassed for 20 minutes under a vacuum of -0.09 MPa until no obvious bubbles are precipitated in the suspension, thus obtaining the contents suspension.

[0082] S2: Preparation of capsule shell solution

[0083] Weigh out the gelatin, glycerin, titanium dioxide and purified water according to the formula, mix them and heat to 75°C to dissolve, and let stand at this temperature to remove bubbles, so as to obtain a uniform and transparent gel solution.

[0084] S3: Shot pressing and drying

[0085] Using a soft capsule pelletizing machine, the prepared content suspension and gel are pelletized, with each capsule containing 500mg of content. The pelleted soft capsules are first set at 20-25℃ for 2 hours, then washed with an ethanol solution, and finally rolled and dried at 25℃ and 30% humidity for 24 hours to obtain the soft capsule product.

[0086] Example 5

[0087] This embodiment provides a soft capsule product based on composition A3 described in Example 3, and its preparation method.

[0088] 1. Raw materials and formula

[0089] (1) Contents formulation:

[0090] Composition A3 (10 parts Coenzyme Q10 + 20 parts Mangosteen Fruit Shell Extract + 20 parts Sea Buckthorn Seed Extract): 50.0g;

[0091] Oily matrix (medium-chain triglycerides, MCT oil): 245.0 g;

[0092] Suspension agent (hydrogenated palm oil): 4.5 g;

[0093] Emulsifier (polyglycerol ricinoleate): 0.5 g;

[0094] Total weight of contents: 300.0 g

[0095] (2) Capsule shell liquid formulation:

[0096] Gelatin: 100.0 g;

[0097] Plasticizer (sorbitol solution): 40.0 g;

[0098] Light-blocking agent (titanium dioxide): 1.5 g;

[0099] Purified water: 110.0 g

[0100] 3. The preparation method of the soft capsules provided in this embodiment is as follows:

[0101] S1: Prepare a suspension of the contents

[0102] (a) Oil phase preparation: Weigh out the MCT oil according to the formula amount and heat it to 68°C. Slowly add the hydrogenated palm oil according to the formula amount while stirring at 350 rpm, continue stirring and keep warm for 25 minutes until the suspending agent is completely dissolved and a clear, transparent oil phase is formed.

[0103] (b) Cooling and emulsification: Cool the oil phase to 38°C. While stirring continuously, slowly add the prescribed amount of polyglycerol ricinoleate and stir for 18 minutes until it is completely and evenly dispersed to obtain the primary emulsion.

[0104] (c) Solid phase addition and dispersion: Under continuous stirring, 50.0 g of composition A3 was slowly and in batches added to the above primary emulsion. After the addition was complete, the stirring speed was increased to 900 rpm and stirred for 30 minutes to fully wet and initially disperse the powder, resulting in a coarse suspension.

[0105] (d) Homogenization: Transfer the coarse suspension into a high-shear homogenizer and homogenize at 4500 rpm for 12 minutes until a uniform, fine suspension without obvious particles is formed.

[0106] (e) Degassing: The homogenized suspension is transferred to a vacuum degassing tank and degassed for 25 minutes under a vacuum of -0.085 MPa until no obvious bubbles are precipitated in the suspension, thus obtaining the contents suspension.

[0107] S2: Preparation of capsule shell solution

[0108] Weigh out the gelatin, sorbitol solution, titanium dioxide and purified water according to the formula, mix them and heat to 70°C to dissolve, and let stand at this temperature to remove bubbles, so as to obtain a uniform and transparent gel solution.

[0109] S3: Shot pressing and drying

[0110] Using a soft capsule pelletizing machine, the prepared content suspension and gel were pelletized, with each capsule containing 500mg of content. The pelleted soft capsules were first set at 22°C for 1.5 hours, then washed with an ethanol solution, and finally rolled and dried at 28°C and 25% humidity for 28 hours to obtain the soft capsule product.

[0111] Test case

[0112] The purpose of this experiment was to verify the effect of the compositions provided in Examples 1-3 on alleviating physical fatigue through animal experiments, to explore the synergistic effect among the components, and the influence of different extraction processes on the effect. In the statistical analysis phase of the experimental results, the experimental data were expressed as mean ± standard deviation. One-way ANOVA was performed using statistical software, and the LSD method was used for inter-group comparisons. P < 0.05 was used as the criterion for statistical significance.

[0113] 1. Experimental Materials

[0114] Laboratory animals: SPF-grade ICR male mice, weighing 18-22g, grouped as follows:

[0115] Coenzyme Q10 monotherapy group (dosage was 100 mg / kg);

[0116] Mangosteen fruit shell extract (single dose: 100 mg / kg);

[0117] Sea buckthorn seed extract (single dose: 100 mg / kg);

[0118] Example 1 Composition A1 (Composition consists of: 10 parts coenzyme Q10 + 40 parts mangosteen fruit shell extract, dosage is 100 mg / kg)

[0119] Example 1 Composition A1-1 (The composition consists of: 5 parts coenzyme Q10 + 45 parts mangosteen peel extract, the dosage is 100 mg / kg, and the extraction method of mangosteen peel extract is the same as in Example 1).

[0120] Example 1 Composition A1-2 (The composition consists of: 20 parts of coenzyme Q10 + 30 parts of mangosteen peel extract, the dosage is 100 mg / kg, and the extraction method of mangosteen peel extract is the same as in Example 1).

[0121] Example 2 Composition A2 (Composition consists of: 10 parts coenzyme Q10 + 40 parts mangosteen fruit shell extract, dosage is 100 mg / kg)

[0122] Example 3 Composition A3 (Composition consists of: 10 parts coenzyme Q10 + 20 parts mangosteen fruit shell extract + 20 parts sea buckthorn seed extract, dosage is 100 mg / kg).

[0123] Blank control group (equal volume of physiological saline).

[0124] 2. Experimental Methods and Results

[0125] Ninety SPF-grade ICR male mice were randomly divided into nine groups of ten each. All mice in each group were administered the drug by gavage for 30 consecutive days. The administration volume was uniformly 0.2 mL / 10 g body weight, and the drug concentration was controlled to ensure the accuracy and consistency of the dosage.

[0126] The weighted swimming experiment was conducted 30 minutes after the last drug administration. Mice were placed in a swimming tank measuring 50cm×50cm×40cm, with a water depth of 30cm and a water temperature controlled at 25±1℃. A lead weight equivalent to 5% of the mouse's body weight was simultaneously loaded onto the mouse's tail. The time from entry into the water until exhaustion was recorded. Exhaustion was defined as the mouse's head being submerged for 10 seconds without surfacing. This was defined as the swimming time. The experimental results are shown in Table 1.

[0127] Table 1 Comparison of swimming time under load among different groups of mice (n=10)

[0128]

[0129] The above experimental results show that, compared with the blank control group, although each single-component group showed a certain extension, the effect was limited, and there was no statistically significant difference in the sea buckthorn seed single-component group. The key finding is that the swimming time (38.2 min) of composition A1 (ratio 10:40) in Example 1 was significantly better than all single-component groups and the control groups with different ratios (A1-1, A1-2), which preliminarily proves that coenzyme Q10 and mangosteen peel extract have the best synergistic effect under specific ratios. Furthermore, the effect of composition A3 (with added sea buckthorn seed extract) in Example 3 (41.5 min) was the most outstanding, significantly better than Example 1, suggesting the synergistic potential of the three-component combination. Meanwhile, the effect of Example 2 (hot reflux method) (33.5 min) was significantly inferior to Example 1 (ultrasound-microwave method), indicating that the extraction process has a decisive influence on the final efficacy.

[0130] Biochemical indicators were measured immediately after the swimming experiment. Blood samples were collected by enucleation, and serum was separated. The following methods were used to measure each indicator: blood lactate (BLA) was measured using the lactate oxidase method; serum urea nitrogen (SUN) using the urease method; liver glycogen using the anthrone method; superoxide dismutase (SOD) using the xanthine oxidase method; and malondialdehyde (MDA) using the thiobarbituric acid method. The experimental results are shown in Table 2.

[0131] Table 2 Comparison of blood biochemical indicators in mice of different groups (n=10)

[0132]

[0133] Data on blood lactate, serum urea nitrogen, and liver glycogen revealed the physiological mechanism of anti-fatigue effects from a metabolic perspective.

[0134] The experimental results showed that composition A1 in Example 1 was significantly more effective than the single-component groups in reducing post-exercise blood lactate (5.2 mmol / L) and serum urea nitrogen (7.1 mmol / L), and increasing energy reserves (liver glycogen) (12.5 mg / g). This confirms that the composition not only delays the onset of fatigue (by reducing the accumulation of metabolic products) but also enhances the body's energy supply. The three indicators of composition A3 in Example 3 were further optimized, demonstrating the positive contribution of the addition of sea buckthorn seed extract to metabolic regulation. Similarly, the effect of Example 2 was between that of the single-component group and Example 1, further confirming the advantages of the ultrasonic-microwave extraction process in obtaining highly active ingredients.

[0135] Table 3 Comparison of antioxidant indices among different groups of mice (n=10)

[0136]

[0137] SOD and MDA data illustrate the antioxidant mechanism of the composition.

[0138] The above experimental results show that composition A1 in Example 1 can most effectively enhance the activity of the body's own antioxidant enzyme SOD (168.5 U / mg prot) and significantly reduce the oxidative damage product MDA (5.1 nmol / mg prot), with better effects than the single composition and Example 2. This indicates that its anti-fatigue effect is closely related to alleviating oxidative stress. Composition A3 in Example 3 showed peak antioxidant performance, suggesting that the antioxidants rich in sea buckthorn seed extract, together with the original combination, produced a strong antioxidant synergistic effect, jointly scavenging more free radicals and protecting cell structure.

[0139] The above-mentioned weighted swimming experiment and the determination of multiple biochemical and antioxidant indicators comprehensively verified the excellent anti-fatigue effect of the composition of the present invention. The experimental results show that:

[0140] The scientific combination of Coenzyme Q10 (which improves mitochondrial function) and mangosteen peel extract (anti-inflammatory and antioxidant) (optimal ratio of 10:40) produces a "1+1>2" effect in terms of extending endurance, optimizing metabolism and anti-oxidation, and its overall performance is significantly better than any single component.

[0141] Secondly, the extraction process of mangosteen shell extract is crucial. Compared with the traditional hot reflux method, the ultrasonic-microwave synergistic extraction method can extract the active ingredients in mangosteen shell more efficiently, thereby directly improving the efficacy of the final product.

[0142] Finally, sea buckthorn seed extract was introduced into the core combination. Its flavonoids and other substances further enhanced the overall antioxidant and energy metabolism regulation capabilities, forming a more powerful triple synergistic network, resulting in Example 3 showing optimal performance across almost all indicators. In summary, this invention, through scientific component selection, optimized extraction processes, and reasonable dosage form design, successfully developed a fatigue-relieving solution with a clear mechanism and significant efficacy.

[0143] The above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the present invention, and all such modifications and substitutions should be covered within the scope of the claims of the present invention. Technical aspects, shapes, and structures not described in detail in this invention are all well-known technologies.

Claims

1. A composition having the function of relieving physical fatigue, characterized in that, Based on parts by weight, it comprises the following raw materials: 10 parts coenzyme Q10 and 40 parts mangosteen peel extract, wherein the mangosteen peel extract is prepared according to the following method: A1: Crushing: Crush the dried mangosteen shells and pass them through a 30-80 mesh sieve; A2: Extraction: Weigh the mangosteen fruit shell powder and add 70% ethanol solution at a mass-volume ratio of 1:8-1:

30. Use an ultrasonic-microwave synergistic extractor at a power of 200-300W and a temperature of 50-60℃ for 20-30 minutes. A3: Separation: Centrifuge the extract at 4000-4500 r / min for 15 minutes, take the supernatant and filter it, and collect the filtrate; A4: Concentration and drying: The filtrate is concentrated under reduced pressure at 50℃-60℃ to recover ethanol, and further concentrated to an extract. The extract is then spray-dried, with the inlet air temperature controlled at 170-185℃ and the outlet air temperature at 75-85℃. After spray drying, the mangosteen shell extract is obtained.

2. A composition having the function of relieving physical fatigue, characterized in that, Based on parts by weight, it comprises the following raw materials: 10 parts coenzyme Q10, 20 parts mangosteen fruit shell extract, and 20 parts sea buckthorn seed extract, wherein the mangosteen fruit shell extract is prepared according to the following method: A1: Crushing: Crush the dried mangosteen shells and pass them through a 30-80 mesh sieve; A2: Extraction: Weigh the mangosteen fruit shell powder and add 70% ethanol solution at a mass-volume ratio of 1:8-1:

30. Use an ultrasonic-microwave synergistic extractor at a power of 200-300W and a temperature of 50-60℃ for 20-30 minutes. A3: Separation: Centrifuge the extract at 4000-4500 r / min for 15 minutes, take the supernatant and filter it, and collect the filtrate; A4: Concentration and drying: The filtrate is concentrated under reduced pressure at 50℃-60℃ to recover ethanol, and further concentrated to extract. The extract is then spray-dried, with the inlet air temperature controlled at 170-185℃ and the outlet air temperature at 75-85℃. After spray drying, the mangosteen shell extract is obtained. The sea buckthorn seed extract was prepared according to the following method: C1: Pretreatment: After crushing the sea buckthorn seeds, pass them through a 40-80 mesh sieve, defatted and dried to obtain pretreated sea buckthorn seed powder; C2: Extraction: Add the pretreated sea buckthorn seed powder to a 60%-80% ethanol solution at a mass-volume ratio of 1:10-1:30 and extract for 20-40 minutes at an ultrasonic power of 300-500W and a temperature of 40-60℃. C3: Post-processing: The obtained extract is subjected to solid-liquid separation, concentration and drying to obtain the sea buckthorn seed extract.

3. The use of the composition according to any one of claims 1-2 in the preparation of food or medicine for relieving physical fatigue.

4. A soft capsule, characterized in that, It comprises the composition according to any one of claims 1-2 and pharmaceutically or food-grade excipients.

5. The soft capsule according to claim 4, characterized in that, It comprises a capsule shell and contents, the contents being made from the following raw materials in parts by weight: The composition according to any one of claims 1-2 comprises 50-80 parts, 20-50 parts, 1-3 parts, 0.5-2 parts, and 0.5-2 parts, respectively.

6. The soft capsule according to claim 5, characterized in that, The oily matrix is ​​one or more of medium-chain triglycerides, soybean oil, and corn oil; the suspending agent is beeswax or hydrogenated vegetable oil; and the emulsifier is soybean lecithin.

7. A method for preparing the soft capsules as described in any one of claims 4-6, characterized in that, Includes the following steps: S1: Preparation of contents: Heat the oily matrix, add a suspending agent to dissolve it, cool it and add an emulsifier, then add the composition according to any one of claims 1-2, and after homogenization and degassing, obtain a contents suspension; S2: Preparation of capsule shell solution: Mix gelatin, plasticizer, opacifier and water, heat to dissolve, degas to obtain the glue solution; S3: Squash compression and drying: The contents suspension and gel are compressed into shape using a soft capsule squashing machine, and then shaped, washed and dried to obtain the soft capsules.

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