Sample introduction and reaction system and method for chip sequencing

Abstract

The invention belongs to the technical field of chip diagnosis, and discloses a sample introduction and reaction system for chip sequencing, the sample introduction and reaction system comprises a supply device, a flow channel switching device, a dismounting device, an extraction device and a reaction tank; the supply device is provided with a sealed detachable opening structure, and the opening detaching device can move relative to the supply device until the detachable opening structure is opened; the supply device is arranged around the flow channel switching device, the extraction device is connected with the bottom end of the supply device and the reaction tank through the flow channel switching device, and the flow channel switching device can enable the supply device to be communicated with the extraction device or enable the extraction device to be communicated with the reaction tank by switching the state of the flow channel switching device. According to the sample introduction and reaction system for chip sequencing, the reagent conveying time can be shortened, the phenomenon of dead volume of a reagent in the conveying process is reduced, the problem of reagent waste caused by bubbles generated by a pipeline in the conveying process is effectively avoided, and the reagent sample introduction efficiency is improved.

Description

Technical Field

[0001] This invention belongs to the field of chip diagnostic technology, specifically relating to a sample introduction and reaction system and method for chip sequencing. Background Technology

[0002] Sequencing instruments require fluid systems to store, extract, and transfer reagents. Typical sequencers directly extract reagents using negative pressure, placing a syringe pump behind the chip in the flow path. The pump provides negative pressure, forcing the target reagent in the reagent container into the chip for reaction. However, this process easily generates air bubbles in the tubing, leading to reagent waste. Furthermore, because there is still a distance between the reagent and the flow cell, dead volume of reagent may exist during long-distance transport. Summary of the Invention

[0003] The purpose of this invention is to provide a sample introduction and reaction system for chip sequencing, which enables the target reagents from the supply device to be smoothly delivered to the reaction cell for reaction, effectively reducing the dead volume of reagents and the generation of bubbles during the delivery process.

[0004] The following technical solutions are used to achieve the above objectives.

[0005] The first aspect of the present invention provides a sample introduction and reaction system for chip sequencing, the sample introduction and reaction system comprising a supply device, a flow channel switching device, a disassembly device, an extraction device and a reaction cell;

[0006] The supply device has a sealed detachable structure, and the detachable device is movable relative to the supply device to open the detachable structure;

[0007] The supply device is arranged around the flow channel switching device, and the extraction device is connected to the bottom of the supply device and the reaction tank through the flow channel switching device. The flow channel switching device can switch its own state to connect the supply device with the extraction device or to connect the extraction device with the reaction tank.

[0008] In some embodiments, the reaction tank includes a carrier and a seal; the carrier has a carrier groove for chip insertion, the seal is elastically connected to the carrier and disposed at the inner end of the carrier groove, the seal can tightly press the inserted chip under its own elastic force and can overcome the elastic force to release the chip under external force; the seal also has a first sealing ring for communicating with the liquid inlet of the chip, the first sealing ring is connected to the flow channel switching device.

[0009] In some embodiments, the sealing element includes a connector, a sealing plate, and an elastic element, with the first sealing ring disposed on the sealing plate; the top of the carrier has a clearance hole, the connector is movably inserted through the clearance hole, and a receiving groove is formed at the bottom of the inner end of the carrier groove; the sealing plate is connected to the carrier through the elastic element, and the sealing plate is connected to the connector; the sealing plate can be stored in the receiving groove when the elastic element is in a compressed state, and extend out of the receiving groove in an expanded state to tightly abut against the inserted chip.

[0010] In some embodiments, a heating device is provided at the bottom of the support groove, the heating device including a heating plate and a heating film, the heating film being disposed below the heating plate; and / or,

[0011] The sample introduction and reaction system also includes a waste liquid collection device; the sealing element is provided with a second sealing ring for communicating with the liquid outlet of the chip, and the second sealing ring is connected to the waste liquid collection device.

[0012] In some embodiments, the supply device includes a reagent container, and the detachable structure is a sealing plug disposed on the top of the reagent container;

[0013] The disassembly device includes a puncture plate and a puncture needle disposed on the puncture plate. The puncture plate is movably suspended above the reagent container. The puncture plate can move relative to the reagent container and drive the puncture needle to puncture and open the sealing plug.

[0014] In some embodiments, the injection and reaction system further includes a housing and a puncture auxiliary component; the supply device and the puncture plate are both disposed within the housing; the puncture auxiliary component includes an auxiliary plate body and a connecting rod connected to the auxiliary plate body, the auxiliary plate body being located outside the housing, and the connecting rod being movably inserted through the housing and connected to the puncture plate.

[0015] In some embodiments, three connecting rods are provided, and the three connecting rods are arranged in a triangular pattern on the auxiliary plate; the piercing plate has three insertion slots that cooperate with the connecting rods.

[0016] In some embodiments, multiple reagent containers are provided, each reagent container is dispersed and arranged around the flow channel switching device; multiple puncture needles are provided on the puncture plate, and each reagent container is arranged in a one-to-one correspondence with a puncture needle.

[0017] In some embodiments, the extraction device includes a syringe, a connecting block, and a driving device; the syringe is vertically arranged and communicates with the flow channel switching device, the syringe has a push rod for compressing or releasing internal space, the driving device is arranged side by side on one side of the syringe, and the output end of the driving device is connected to the push rod of the syringe through the connecting block.

[0018] A second aspect of the present invention provides a sample introduction and reaction method for chip sequencing, using the sample introduction and reaction system for chip sequencing as described above, comprising the following steps:

[0019] The control device moves relative to the supply device to open the detachable structure of the supply device, so that the supply device can be connected to the external air pressure.

[0020] The flow channel switching device switches its own state so that the supply device is connected to the extraction device through the flow channel switching device, and the extraction device extracts the fluid in the supply device and stores it temporarily.

[0021] Subsequently, the flow channel switching device switches its own state, so that the extraction device is connected to the reaction tank through the flow channel switching device, and the fluid temporarily stored in the extraction device flows into the reaction tank through the flow channel switching device to react.

[0022] The technical solution provided by this invention has the following advantages and effects:

[0023] This sample introduction and reaction system for chip sequencing improves structural integration by arranging the supply device around the flow channel switching device and shortening the distance between the supply device and the flow channel switching device. This reduces reagent delivery time and minimizes the formation of dead volume in the reagents during delivery. Furthermore, the connection between the bottom of the supply device and the flow channel switching device allows the reagents to fully enter the reaction chamber when the detachable structure of the supply device is open, effectively preventing reagent waste caused by incomplete aspiration. Through the coordinated action of the flow channel switching device and the extraction device, the reagents are delivered from the supply device to the reaction chamber via a process of first aspiration under negative pressure and then push under positive pressure. This effectively avoids the problem of reagent waste caused by air bubbles in the tubing during delivery and improves reagent introduction efficiency. Attached Figure Description

[0024] Figure 1 This is a schematic diagram of the overall structure of the sample introduction and reaction system for chip sequencing according to an embodiment of the present invention;

[0025] Figure 2 yes Figure 1 A schematic diagram of the local structure of the sample introduction and reaction system used for chip sequencing;

[0026] Figure 3 yes Figure 2 A top view of a local structure of the sample introduction and reaction system used for chip sequencing;

[0027] Figure 4 This is a partial structural schematic diagram of the reaction tank according to an embodiment of the present invention;

[0028] Figure 5 yes Figure 4 A schematic diagram of the reaction tank from another angle;

[0029] Figure 6 yes Figure 4 A schematic diagram of the structure of the reaction cell in the uninserted state of the chip;

[0030] Figure 7 This is a schematic diagram of the base structure according to an embodiment of the present invention;

[0031] Figure 8 This is a structural diagram of the cooperation between the auxiliary plate and the shell in an embodiment of the present invention;

[0032] Figure 9 This is a schematic diagram of the extraction device according to an embodiment of the present invention.

[0033] Explanation of reference numerals in the attached figures:

[0034] 100. Sample introduction and reaction systems for chip sequencing;

[0035] 1. Supply device; 11. Reagent container; 2. Flow channel switching device; 3. Disassembly device; 31. Puncture plate; 32. Puncture needle; 33. Auxiliary plate; 34. Connecting rod; 4. Extraction device; 41. Syringe; 42. Connecting block; 43. Driving device; 5. Reaction tank; 51. Supporting component; 511. Supporting groove; 52. Heating device; 53. Sealing component; 531. Second sealing ring; 532. First sealing ring; 533. Connecting component; 534. Sealing plate; 535. Elastic component; 7. Shell; 71. Base; 72. Cover;

[0036] 200. Chip. Detailed Implementation

[0037] To facilitate understanding of the present invention, specific embodiments of the present invention will be described in more detail below with reference to the accompanying drawings.

[0038] Unless otherwise specified or defined, the terms "first," "second," etc., used in this document are for distinguishing names only and do not represent a specific number or order.

[0039] Unless otherwise stated or defined, the term “and / or” as used herein includes any and all combinations of one or more of the associated listed items.

[0040] It should be noted that in this article, "fixed to" or "connected to" can mean directly fixed to or connected to a component, or indirectly fixed to or connected to a component.

[0041] This invention provides a sample introduction and reaction system 100 for chip sequencing, such as... Figures 1 to 9 As shown, it includes a supply device 1, a flow channel switching device 2, a disassembly device 3, an extraction device 4, and a reaction tank 5.

[0042] The supply device 1 has a sealed, detachable structure, and the detachable device 3 is movable relative to the supply device 1 to open the detachable structure. The supply device 1 is used to store reagents required for sequencing of the chip 200, such as reaction solutions. The supply device 1 is normally sealed to ensure that the reagents will not be drawn out when the chip 200 sequencing operation is not being performed. When the chip 200 sequencing sample injection operation is required, the detachable device 3 can be opened to allow the reagents to be drawn out and flow normally, thereby effectively controlling the outflow of reagents.

[0043] The supply device 1 is arranged around the flow channel switching device 2, and the extraction device 4 is connected to the bottom of the supply device 1 and the reaction tank 5 through the flow channel switching device 2. The flow channel switching device 2 can switch its own state to connect the supply device 1 with the extraction device 4, or connect the extraction device 4 with the reaction tank 5. The supply device 1, flow channel switching device 2, and extraction device 4 all have pipe joints. The pipe joint of the supply device 1 is connected to the pipe joint of the flow channel switching device 2 through a pipeline, and the flow channel switching device 2 and extraction device 4 are also connected through pipe joints and pipelines. Specifically, when the supply device 1 is connected to the extraction device 4 through the flow channel switching device 2, the extraction device 4 can extract the fluid in the supply device 1 and temporarily store it. After extraction is completed, the extraction device 4 switches to be connected to the reaction tank 5 through the flow channel switching device 2, and the fluid temporarily stored in the extraction device 4 can flow into the reaction tank 5 through the flow channel switching device 2 to react. The supply device 1 is arranged around the flow channel switching device 2, which improves the integration of the structure and shortens the distance between the supply device 1 and the flow channel switching device 2. The bottom end of the supply device 1 is connected to the flow channel switching device 2, so that when the detachable structure of the supply device 1 is in the open state, the reagent can be completely entered into the reaction tank 5 for reaction. Furthermore, through the cooperation of the flow channel switching device 2 and the extraction device 4, the reagent can be transported from the supply device 1 to the reaction tank 5 by first negative pressure suction and then positive pressure push.

[0044] Therefore, based on the sample introduction and reaction system 100 for chip sequencing described above, the sample introduction and reaction method includes the following steps:

[0045] The control device 3 moves relative to the supply device 1 to open the detachable structure of the supply device 1, so that the supply device 1 is connected to the external air pressure.

[0046] The flow channel switching device 2 switches its own state so that the supply device 1 is connected to the extraction device 4 through the flow channel switching device 2, and the extraction device 4 extracts the fluid in the supply device 1 and temporarily stores it.

[0047] Subsequently, the flow channel switching device 2 switches its own state, so that the extraction device 4 is connected to the reaction tank 5 through the flow channel switching device 2, and the fluid temporarily stored in the extraction device 4 flows into the reaction tank 5 through the flow channel switching device 2 to react.

[0048] In summary, the sample introduction and reaction system 100 for chip sequencing, with the supply device 1 arranged around the flow channel switching device 2, improves the structural integration and shortens the distance between the supply device 1 and the flow channel switching device 2. This reduces reagent delivery time and minimizes the formation of dead volume in the reagents during delivery. Furthermore, the connection between the bottom of the supply device 1 and the flow channel switching device 2 allows the reagents to fully enter the reaction chamber 5 when the detachable structure of the supply device 1 is open, effectively preventing reagent waste caused by incomplete aspiration. Moreover, the cooperation between the flow channel switching device 2 and the extraction device 4 enables the reagents to be delivered from the supply device 1 to the reaction chamber 5 via negative pressure aspiration followed by positive pressure push, effectively avoiding the problem of reagent waste caused by air bubbles in the tubing during delivery and improving reagent introduction efficiency.

[0049] In some embodiments, such as Figures 4 to 6As shown, the reaction tank 5 includes a support member 51 and a sealing member 53. The support member 51 is provided with a support groove 511 for inserting the chip 200. The sealing member 53 is elastically connected to the support member 51 and is disposed at the inner end of the support groove 511. The sealing member 53 can tightly press the inserted chip 200 under its own elastic force and can overcome the elastic force to release the chip 200 under the action of external force. The sealing member 53 is also provided with a first sealing ring 532 for communicating with the liquid inlet hole of the chip 200. The first sealing ring 532 is connected to the flow channel switching device 2. It should be noted that the outer end of the support groove 511 is the insertion interface of the chip 200, and the inner end is the end away from the insertion interface of the chip 200. By setting the sealing element 53 at the inner end of the carrier groove 511, when the chip 200 needs to be inserted for sequencing, the sealing element 53 moves away from the carrier groove 511 under the action of external force, making it easier for the chip 200 to be inserted into the carrier groove 511. After the chip 200 is inserted, the sealing element 53 is released, and the sealing element 53 can press the chip 200 under its own elastic force, so that the first sealing ring 532 on the sealing element 53 is in sealed contact with and connected to the liquid inlet hole of the chip 200, so that the reagent can flow smoothly into the chip 200 for reaction after passing through the flow channel switching device 2 and the first sealing ring 532 in sequence. It has the characteristics of simple structure and smooth flow.

[0050] In some embodiments, such as Figures 4 to 6As shown, the sealing element 53 includes a connector 533, a sealing plate 534, and an elastic element 535. The first sealing ring 532 is disposed on the sealing plate 534. The top of the support member 51 has a clearance hole, and the connector 533 is movably inserted through the clearance hole. The bottom of the inner end of the support groove 511 has a receiving groove. The sealing plate 534 is connected to the support member 51 through the elastic element 535, and the sealing plate 534 is connected to the connector 533. The sealing plate 534 can be stored in the receiving groove when the elastic element 535 is in a compressed state, and extend out of the receiving groove in an expanded state to tightly abut against the inserted chip 200. The elastic element 535 can be a spring or other components with elastic properties, and two or more can be provided; no particular limitation is made here. By setting the connector 533 to extend beyond the support member 51, it is convenient to apply external force. Applying external force to the connector 533 will cause the sealing plate 534 to move downwards against the elastic force of the elastic member 535 and be housed in the receiving groove. At this time, the chip 200 can be inserted, and the sealing plate 534 will not obstruct the insertion of the chip 200. After the chip 200 is inserted, the external force is removed, and the sealing plate 534 springs upwards under the elastic force of the elastic member 535 to tightly press the chip 200 against the top of the support groove 511. At this time, the first sealing ring 532 on the sealing plate 534 is in sealed contact with and connected to the liquid inlet of the chip 200. In some embodiments, such as... Figure 5 As shown, a heating device 52 is provided at the bottom of the support groove 511. The heating device 52 includes a heating plate and a heating film, with the heating film disposed below the heating plate. The heating film generates heat to heat the heating plate, which then transfers heat to the chip 200 to heat it to a preset temperature.

[0051] In some embodiments, such as Figure 4 As shown, the sample introduction and reaction system also includes a waste liquid collection device disposed on the housing 7; the sealing member 53 is provided with a second sealing ring 531 for communicating with the liquid outlet of the chip 200, and the second sealing ring 531 is connected to the waste liquid collection device. It should be noted that the conventional chip 200 has a flow channel and a liquid inlet and a liquid outlet communicating with the flow channel. Specifically, by providing the second sealing ring 531 to connect the liquid outlet of the chip 200 and the waste liquid collection device, the second sealing ring 531 is connected to the waste liquid collection device through a pipeline, allowing the waste liquid from the reaction on the flow channel of the chip 200 to be discharged into the waste liquid collection device for collection, thereby integrating sample introduction, reaction, and waste liquid discharge into a single operation in the sample introduction and reaction system. It should be noted that the sealing plate 534 is a downwardly recessed stepped structure, and both the first sealing ring 532 and the second sealing ring 531 are disposed in the recess of the sealing plate 534 so that the chip 200 abuts against the step of the sealing plate 534 when inserted into place.

[0052] In some embodiments, such as Figure 2 As shown, the supply device 1 includes a reagent container 11, and the detachable structure is a sealing plug disposed on the top of the reagent container 11. The detachable device 3 includes a puncture plate 31 and a puncture needle 32 disposed on the puncture plate 31. The puncture plate 31 is movably suspended above the reagent container 11. The puncture plate 31 can move relative to the reagent container 11 and drive the puncture needle 32 to puncture and open the sealing plug. The sealing plug is a rubber stopper, which can effectively seal the top opening of the reagent container 11 under normal conditions. The puncture needle 32 can quickly puncture the rubber stopper. Therefore, by applying external force to the puncture plate 31, causing the puncture plate 31 to move downwards, the puncture needle 32 can puncture the rubber stopper, allowing the reagent container 11 to be vented and the reagent to flow normally, so that it can flow to the reaction tank 5 under the suction of the extraction device 4.

[0053] In some embodiments, such as Figure 8 As shown, the sample injection and reaction system also includes a housing 7 and a puncture auxiliary component; the supply device 1 and the puncture plate 31 are both disposed within the housing 7; the puncture auxiliary component includes an auxiliary plate 33 and a connecting rod 34 connected to the auxiliary plate 33. The auxiliary plate 33 is located outside the housing 7, and the connecting rod 34 is movably inserted through the housing 7 and connected to the auxiliary plate 33. The supply device 1 is disposed within the housing 7, which provides protection. External force applied to the auxiliary plate 33 outside the housing 7 can move the puncture plate 31 within the housing 7 to perform the puncture operation.

[0054] Specifically in this embodiment, such as Figure 8 As shown, three connecting rods 34 are provided, arranged in a triangular pattern on the auxiliary plate 33; the puncture plate 31 has three insertion slots that mate with the connecting rods 34. The triangular structure formed by the three connecting rods 34 ensures that when external force is applied to the auxiliary plate 33, the puncture plate 31 experiences uniform force, thus allowing it to move downwards evenly for the puncture operation.

[0055] In some embodiments, such as Figure 2As shown, multiple reagent containers 11 are provided, each dispersed and arranged around the flow channel switching device 2; multiple puncture needles 32 are provided on the puncture plate 31, and each reagent container 11 corresponds to one puncture needle 32. Multiple reagent containers 11 can simultaneously hold multiple reaction reagents, and each puncture needle 32 is synchronously driven by the puncture plate 31 to perform puncture, which can further improve sample injection efficiency. The puncture plate 31 is U-shaped, and the flow channel switching device 2 falls into the U-shaped hollow area of ​​the puncture plate 31 to avoid interference with the puncture operation of the puncture plate 31.

[0056] In some embodiments, such as Figure 2 As shown, the flow channel switching device 2 is a multi-channel switching valve, which can quickly switch between multiple channels to connect different reagent containers 11 to the extraction device 4, or to connect the extraction device 4 to the reaction tank 5.

[0057] In some embodiments, such as Figure 9 As shown, the extraction device 4 includes a syringe 41, a connecting block 42, and a driving device 43. The syringe 41 is vertically arranged and communicates with the flow channel switching device 2. The syringe 41 has a push rod for compressing or releasing the internal space. The driving device 43 is arranged side by side on one side of the syringe 41, and the output end of the driving device 43 is connected to the push rod of the syringe 41 through the connecting block 42. Specifically, the syringe 41 is a conventional syringe 41. The injection port of the syringe 41 is communicated with the flow channel switching device 2. Pushing the push rod of the syringe 41 compresses or releases the internal space to achieve the purpose of ejecting or drawing out the reagent. The driving device 43 drives the push rod to move up and down inside the syringe 41 to draw out or eject the reagent. Specifically, in this embodiment, the syringe 41 is disposed on the housing 7, and the driving device 43 is disposed outside the housing 7. The driving device 43 is connected to the push rod of the syringe 41 through the connecting block 42 to avoid vibration of the driving device 43 during operation, which would affect the liquid injection operation. The connecting block 42 has a Z-shaped structure.

[0058] In some embodiments, such as Figure 2 As shown, the extraction device 4 and the reaction tank 5 are located on the same side of the flow channel switching device 2, and the height of the reaction tank 5 is lower than the height of the flow channel switching device 2. This facilitates the smooth flow of the reaction reagent extracted by the extraction device 4 into the reaction tank 5 via the flow channel switching device 2.

[0059] In some embodiments, such as Figure 1 As shown, the housing 7 includes a base 71 and a cover 72. The supply device 1, the flow channel switching device 2, the disassembly device 3, the extraction device 4, and the reaction tank 5 are all adapted to be disposed on the base 71, and the cover 72 is snapped onto the base 71.

[0060] The above embodiments are not an exhaustive list based on the present invention, and there may be many other embodiments not listed. Any substitutions and improvements made without departing from the concept of the present invention are within the protection scope of the present invention.

Claims

1. A sample injection and reaction system for chip sequencing, characterized by, The sample injection and reaction system comprises a supply device, a flow channel switching device, a dismounting device, a suction device and a reaction pool; The supply device has a sealed dismountable structure, and the dismounting device is movable relative to the supply device to open the dismountable structure; The supply device is arranged around the flow channel switching device, and the suction device is connected with the bottom end of the supply device and the reaction pool through the flow channel switching device, which can switch its state to make the supply device communicate with the suction device or make the suction device communicate with the reaction pool.

2. The injection and reaction system of claim 1, wherein, The reaction pool comprises a carrier and a sealing member; the carrier is provided with a carrier groove for chip insertion, and the sealing member is elastically connected to the carrier and arranged at the inner end of the carrier groove; the sealing member can tightly press the inserted chip under the action of its elastic force and can loosen the chip under the action of external force; the sealing member is further provided with a first sealing ring for communicating with the liquid inlet hole of the chip, and the first sealing ring communicates with the flow channel switching device.

3. The sample introduction and reaction system of claim 2, wherein, The sealing member comprises a connecting member, a sealing plate and an elastic member, and the first sealing ring is arranged on the sealing plate; the top of the carrier is provided with an emptying hole, the connecting member is movably arranged in the emptying hole, and the inner end of the carrier groove is provided with a receiving groove; the sealing plate is connected to the carrier through the elastic member, and the sealing plate is connected to the connecting member; the sealing plate can be received in the receiving groove when the elastic member is in a compressed state, and the sealing plate can be extended out of the receiving groove to tightly abut against the inserted chip when the elastic member is in a relaxed state.

4. The sample introduction and reaction system of claim 2, wherein, The bottom of the carrier groove is provided with a heating device, and the heating device comprises a heating plate and a heating film, and the heating film is arranged below the heating plate; and / or, The sample injection and reaction system further comprises a waste liquid collecting device; the sealing member is provided with a second sealing ring for communicating with the liquid outlet hole of the chip, and the second sealing ring communicates with the waste liquid collecting device.

5. The injection and reaction system of any one of claims 1-4, wherein, The supply device comprises a reagent container, and the dismountable structure is a sealing plug arranged at the top of the reagent container; The dismounting device comprises a puncture plate and a puncture needle arranged on the puncture plate; the puncture plate is movably arranged above the reagent container, and the puncture plate can move relative to the reagent container and drive the puncture needle to puncture to open the sealing plug.

6. The sample introduction and reaction system of claim 5, wherein, The sample injection and reaction system further comprises a shell and a puncture auxiliary member; the supply device and the puncture plate are arranged in the shell; the puncture auxiliary member comprises an auxiliary plate body and a connecting rod connected with the auxiliary plate body; the auxiliary plate body is located outside the shell, and the connecting rod is movably arranged in the shell and connected with the puncture plate.

7. The sample introduction and reaction system of claim 6, wherein, The connecting rod is provided with three, and the three connecting rods are triangularly arranged on the auxiliary plate body; the puncture plate is provided with three insertion grooves matched with the connecting rods.

8. The sample introduction and reaction system of claim 5, wherein, The reagent containers are provided in plurality, each of which is dispersed and arranged around the flow channel switching device; the puncture plate is provided with a plurality of puncture needles, and the reagent containers are arranged one by one with the puncture needles.

9. The injection and reaction system of any one of claims 1-4, wherein, The extraction device comprises a syringe, a connecting block and a driving device; the syringe is vertically arranged and communicated with the flow channel switching device, the syringe has a push rod for compressing or releasing the internal space, the driving device is arranged side by side on one side of the syringe, and the output end of the driving device is connected with the push rod of the syringe through the connecting block.

10. A method for sample injection and reaction for chip sequencing, characterized by, Using the sample injection and reaction system for chip sequencing according to any one of claims 1-9, comprising the following steps: The detachable opening device is moved relative to the supply device to open the detachable opening structure of the supply device, so that the supply device is communicated with the external air pressure; The flow channel switching device switches its state, so that the supply device is communicated with the extraction device through the flow channel switching device, the extraction device extracts the fluid in the supply device and temporarily stores it; Then the flow channel switching device switches its state, so that the extraction device is communicated with the reaction pool through the flow channel switching device, and the fluid temporarily stored in the extraction device flows into the reaction pool through the flow channel switching device for reaction.

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