Wide-volume automatic liquid treatment platform device compatible with multiple samples and multiple reagent bottles and treatment process

Abstract

The invention provides a wide-volume automatic liquid treatment platform device compatible with multiple samples and multiple reagent bottles and a treatment flow, and relates to the technical field of automatic pipetting. The platform comprises a sample area, an array injection pump, a three-coordinate gantry, a reagent area and a control and display system, wherein the sample area is used for placing sample bottles; the array injection pump comprises an injection pump, a sample pipeline, a two-way medium valve, a multi-way connector, a sample suction pipeline and a sample injection pipeline, one end of the sample pipeline is inserted into the sample bottle, the other end of the sample pipeline is connected with the multi-way connector, and the two-way medium valve is arranged on the sample pipeline and used for opening and closing the sample pipeline. One end of the sample suction pipeline and one end of the sample injection pipeline are respectively connected with a sample suction joint and a sample injection joint on the injection pump, the other end of the sample suction pipeline is connected with a multi-way joint, and the other end of the sample injection pipeline is fixed on a suspended pipetting needle; the three-coordinate gantry comprises a pipetting needle head and a three-coordinate electric cylinder gantry, and the pipetting needle head is mounted on the three-coordinate electric cylinder gantry; the reagent area comprises a reagent bottle, and the reagent bottle receives the liquid sample transferred from the injection pump.

Description

TECHNICAL FIELD

[0001] The present application relates to the field of automatic pipetting technology, in particular, especially to a multi-sample and multi-reagent bottle compatible wide-volume automatic liquid processing platform device and processing flow, which is applied to an analytical laboratory and can realize liquid transfer or preparation functions. BACKGROUND

[0002] In a chemical analysis laboratory, manual operation has been difficult to meet the requirements of high efficiency, accuracy and safety in the analysis and detection process in the face of complex analysis objects and a large number of repetitive samples. Therefore, it has become an inevitable direction of technological development to realize the automation of part or the whole process of analysis operation.

[0003] In liquid sample analysis and detection, pipetting operation is one of the basic experimental steps, and its core function is to realize high-precision delivery and transfer of liquid samples. According to the degree of automation, pipetting devices are mainly divided into three categories: manual, semi-automatic and fully automatic: (1) Manual device: microsyringe is suitable for microliter to milliliter level small volume and high precision pipetting. Pipette is mainly used for large volume pipetting (such as common 10-50mL). (2) Semi-automatic device: handheld pipetting gun is usually used for rapid pipetting of liquid below 10mL. (3) Fully automatic device: array pipetting platform (CN202110461534.4, CN202211254793.0, CN200420093039.4) or liquid preparation workstation (CN202311653677.0, CN200820107586.1) and the like are suitable for batch preparation of liquid samples of various volumes. At present, most pipetting devices (especially semi-automatic and fully automatic multi-channel equipment) adopt air cushion type pipetting plunger pump technology, and are often matched with multi-well plate reagent kits to meet the liquid processing needs of multiple reagent bottles. However, this technology has an inherent defect: there is an air cushion between the liquid and the sealing component of the pump. When pipetting volatile liquid samples, this air cushion may cause vapor pressure changes, thereby affecting pipetting accuracy and causing data deviation. In addition, to avoid cross contamination, the suction head needs to be frequently replaced.

[0004] In liquid sample analysis and detection, multiple indicators or multiple items of the sample often need to be detected. This process involves multiple transfers of liquid samples of different volumes. The existing pipetting method faces significant challenges: using a pipette, different capacity pipettes need to be frequently replaced and manually calibrated, which is tedious. Using a handheld pipetting gun device, disposable suction heads of different ranges need to be repeatedly replaced to meet the accuracy requirements; when pipetting large volumes, multiple repeated operations are often required, resulting in error accumulation and reduced accuracy. Using a liquid preparation workstation, most existing commercial devices are based on a multi-channel pipetting gun array, and the pipetting process is similar to that of a single-channel handheld pipetting gun array, which can realize standardized liquid transfer, but it is difficult to effectively adapt to the complex pipetting needs of different volume ranges and multiple types of reagent bottles.

[0005] In the multi-index analysis or solution preparation for samples, the traditional method needs to transfer samples for multiple times, and there are problems of repeated and tedious operation, difficult to guarantee liquid volume precision and repeatability, poor reagent bottle compatibility, large consumption of pipetting consumables, high detection cost and high experience requirement of operators.

[0006] To solve the above challenges, we invented a multi-sample, multi-reagent bottle compatible wide volume automatic liquid handling platform device and processing flow. SUMMARY

[0007] According to the above, in the multi-index analysis or solution preparation for samples, the traditional method needs to transfer samples for multiple times, and there are problems of repeated and tedious operation, difficult to guarantee liquid volume precision and repeatability, poor reagent bottle compatibility, large consumption of pipetting consumables, high detection cost and high experience requirement of operators, and a multi-sample, multi-reagent bottle compatible wide volume automatic liquid handling platform device and processing flow are provided. The present application solves the problems of multiple transfers of liquid samples and compatibility of multiple types of reagent bottles in the operation of multi-index analysis or solution preparation of samples by coordinating the actions of array syringe pump, two-way medium valve and three-coordinate electric cylinder gantry, and realizes fully automatic and efficient pipetting operation.

[0008] The technical means adopted by the present application are as follows: A multi-sample, multi-reagent bottle compatible wide volume automatic pipetting platform device, comprising a sample area, an array syringe pump, a three-coordinate gantry, a reagent area, a control and display system: The sample area is used for placing sample bottles; The array syringe pump comprises a syringe pump, a sample pipeline, a two-way medium valve, a multi-way connector, a sample suction pipeline and a sample injection pipeline, one end of the sample pipeline is inserted into a sample bottle, the other end is connected to the multi-way connector, the two-way medium valve is arranged on the sample pipeline for opening and closing the sample pipeline, one end of the sample suction pipeline and the sample injection pipeline is respectively connected to the sample suction connector and the sample injection connector on the syringe pump, the other end of the sample suction pipeline is connected to the multi-way connector, and the other end of the sample injection pipeline is fixed to a suspended pipetting needle; The three-coordinate gantry comprises a pipetting needle and a three-coordinate electric cylinder gantry, and the pipetting needle is installed on the three-coordinate electric cylinder gantry; The reagent area comprises reagent bottles, and the reagent bottles receive liquid samples transferred from the syringe pump; The control and display system comprises a control display, and provides a man-machine interface and automatic control.

[0009] Further, the sample area, the reagent area and the array syringe pump are all placed in the working area below the three-coordinate gantry, and the control and display system is placed on the side of the three-coordinate gantry.

[0010] Further, the reagent area can be placed only under the three-coordinate gantry, and the sample area, array injection pump and control and display system are placed around the periphery of the three-coordinate gantry.

[0011] Further, the three-coordinate electric cylinder gantry is an H-shaped mechanical structure, and the pipetting needle is installed on the middle cross beam of the H-shaped structure, and the three-coordinate gantry can realize three-dimensional movement of the pipetting needle in x, y and z axes.

[0012] The application also provides a processing flow of a multi-sample and multi-reagent bottle compatible wide-volume automatic pipetting platform device, including a single-sample dispensing flow, a multi-sample and multi-reagent bottle dispensing flow and a solution preparation flow.

[0013] Further, the single-sample dispensing flow includes the following steps: (1) Preparation: placing a single sample at a designated position of the sample area, placing a target reagent bottle at a corresponding position of the reagent area, and recording the position information; (2) Parameter setting: setting the specification and required dispensing volume of each position reagent bottle through the control and display system; (3) Pipeline cleaning: moving the pipetting needle to a dedicated emptying position by the three-coordinate gantry, keeping the two-way medium valve corresponding to the sample bottle in a normally open state, driving the injection pump to suck and empty at least one cycle of sample to clean the pipeline and the injection pump cavity; (4) Execution of dispensing: moving the pipetting needle above the target reagent bottle by the three-coordinate gantry, lowering the Z-axis to a preset height according to the specification of the reagent bottle, and automatically selecting and combining the optimal injection pump range according to the set dispensing volume by the control and display system to efficiently complete the liquid transfer under the premise of ensuring accuracy; (5) Sequential execution: if there are two or more dispensing reagent bottles, repeat step (4) until all reagent bottles are processed.

[0014] Further, the multi-sample and multi-reagent bottle dispensing flow includes the following steps: (1) Preparation: placing multiple samples at different positions of the sample area and multiple reagent bottles at corresponding positions of the reagent area; (2) Parameter setting: setting three key parameters for each dispensing task through the control and display system: target reagent bottle position and its specification, dispensing volume, and corresponding sample source position; (3) Sequential execution: the system executes according to the set sequence: a. Open the two-way medium valve corresponding to the first sample; b. Perform pipeline cleaning of the sample; c. Perform dispensing operation of the sample to all target reagent bottles; d. Close the two-way medium valve of the current sample source and open the two-way medium valve of the next sample source; e. Repeat steps b-d until all samples are processed.

[0015] Further, the solution preparation process comprises the following steps: (1) Preparation: Place multiple samples at different positions in the sample area, and place multiple reagent bottles at corresponding positions in the reagent area; (2) Parameter setting: Set three key parameters for each dispensing task through the control and display system: target reagent bottle position and its specifications, dispensing volume, and corresponding sample source position; (3) Sequential execution: The system executes according to the set sequence: a. Open the two-way medium valve corresponding to the first sample; b. Perform pipeline cleaning for the sample; c. Perform dispensing operation of the sample to all target reagent bottles; d. Close the two-way medium valve of the current sample source and open the two-way medium valve of the next sample source; e. Repeat steps b-d until all samples are transferred into the same target reagent bottle, achieving mixing and preparation of the solution.

[0016] Compared with the prior art, the present application has the following advantages: 1. Wide volume and high precision pipetting: Array syringe pumps are used to realize liquid transfer, and by combining syringe pumps of different ranges, a wide range of volumes (such as 0.1 mL-100 mL and above) can be covered for high-precision liquid handling, especially suitable for large-volume and high-precision application scenarios. The syringe pump directly sucks and discharges liquid based on the piston displacement principle, with no intermediate air cushion throughout the process, completely avoiding the influence of vapor pressure, and ensuring the accuracy of volatile and non-volatile liquid sample transfer.

[0017] 2. Flexible processing of multiple samples / multiple reagent bottles: The three-coordinate gantry and two-way valve are cooperatively controlled, combined with the arrayed layout of the sample area and the reagent area, to realize efficient and automated access and liquid transfer of multiple sample sources and multiple reagent bottles in a wide range.

[0018] 3. Simplified structure and anti-pollution design: The pipeline is used to transfer liquid, simplifying the mechanical structure. The pipette needle is designed in a suspended manner, effectively avoiding cross contamination caused by liquid dripping.

[0019] 4. Compatibility with multiple specifications of reagent bottles: Through the three-coordinate gantry with adjustable Z-axis height and the modular / replaceable bottle holder design, most different specifications and types of sample bottles and reagent bottles in the analysis laboratory can be easily adapted.

[0020] The platform can efficiently perform single sample dispensing, multi-sample multi-reagent bottle dispensing and complex solution preparation and other liquid processing tasks, covering a wide range of volumes. It has a simple structure, convenient operation, high processing efficiency, excellent precision, and no consumables, and has a broad popularization and application prospect. BRIEF DESCRIPTION OF DRAWINGS

[0021] In order to more clearly illustrate the technical solutions in the embodiments of the present application or the prior art, the drawings needed to be used in the embodiments or the prior art description will be briefly introduced. Obviously, the drawings in the following description are some embodiments of the present application, and those skilled in the art can obtain other drawings according to these drawings without creative labor.

[0022] Figure 1 It is a schematic diagram of the structure of a multi-sample, multi-reagent bottle compatible wide volume automatic liquid processing platform device.

[0023] Figure 2 It is a 3D structure schematic diagram of a multi-sample, multi-reagent bottle compatible wide volume automatic liquid processing platform device.

[0024] Figure 3 It is a structure schematic diagram of a sample area and a reagent area.

[0025] In the figure: 1, sample bottle; 2, two-way medium valve; 3, sample pipeline; 4, multi-way connector; 5, sample suction connector; 6, sample injection connector; 7, injection pump; 8, sample suction pipeline; 9, sample injection pipeline; 10, pipette needle; 11, three-coordinate electric cylinder gantry; 12, reagent bottle; 13, bottle holder; 14, bottle holder tray; 15, control display. DETAILED DESCRIPTION

[0026] It should be noted that the embodiments in the present application and the features in the embodiments can be combined with each other without conflict. The present application will be described in detail below with reference to the drawings and in combination with the embodiments.

[0027] In order to make the purpose, technical solutions and advantages of the embodiments of the present application clearer, the technical solutions in the embodiments of the present application will be described clearly and completely below in combination with the drawings in the embodiments of the present application. Obviously, the described embodiments are only some of the embodiments of the present application, not all. The description of the at least one exemplary embodiment is actually only illustrative, not as any limitation on the present application and its application or use. Based on the embodiments in the present application, all other embodiments obtained by those skilled in the art without creative labor are within the scope of protection of the present application.

[0028] It should be noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to limit the scope of exemplary embodiments according to the invention. As used herein, the singular form is intended to include the plural form as well, unless the context clearly indicates otherwise. Furthermore, it should be understood that when the terms "comprising" and / or "including" are used in this specification, they indicate the presence of features, steps, operations, devices, components, and / or combinations thereof.

[0029] Unless otherwise specifically stated, the relative arrangement, numerical expressions, and values ​​of the components and steps described in these embodiments do not limit the scope of the invention. It should also be understood that, for ease of description, the dimensions of the various parts shown in the drawings are not drawn to actual scale. Techniques, methods, and devices known to those skilled in the art may not be discussed in detail, but where appropriate, such techniques, methods, and devices should be considered part of the specification. In all examples shown and discussed herein, any specific values ​​should be interpreted as merely exemplary and not as limitations. Therefore, other examples of exemplary embodiments may have different values. It should be noted that similar reference numerals and letters in the following figures denote similar items; therefore, once an item is defined in one figure, it need not be further discussed in subsequent figures.

[0030] like Figures 1-3 As shown, the present invention provides a wide-volume automated pipetting platform device compatible with multiple samples and reagent bottles, including a sample area 21, an array injection pump 22, a three-coordinate gantry 23, a reagent area 25, and a control and display system 24.

[0031] The sample area 21 is an area for placing the sample bottle 1, and contains the sample bottle 1. The array injection pump 22 includes the injection pump 7, the sample pipeline 3, the two-way medium valve 2, the multi-way joint 4, the sample suction pipeline 8, and the sample injection pipeline 9. The injection pump 7 is a multi-range injection pump, which is used to realize the transfer of the liquid sample. One end of the sample pipeline 3 is inserted into the sample bottle 1, and the other end is connected to the multi-way joint 4. The two-way medium valve 2 is arranged on the sample pipeline 3, and is used to open and close the sample pipeline 3. One end of the sample suction pipeline 8 and the sample injection pipeline 9 is connected to the sample suction joint 5 and the sample injection joint 6 of the injection pump 7, respectively. The other end of the sample suction pipeline 8 is connected to the multi-way joint 4, and the other end of the sample injection pipeline 9 is fixed to the suspended pipette needle 10. The three-coordinate gantry 23 includes the pipette needle 10 and the three-coordinate electric cylinder gantry 11. The three-coordinate electric cylinder gantry 11 is an H-shaped mechanical structure, and the pipette needle 10 is installed on the middle crossbeam of the H-shaped structure. The three-coordinate gantry 23 can realize the three-dimensional movement of the pipette needle 10 in the x, y, and z axes. The reagent area 25 is an area for placing the reagent bottle 12, and includes the reagent bottle 12, the bottle holder 13, and the bottle holder tray 14. The bottle holder 13 is arranged in an array on the bottle holder tray 14, and the reagent bottle 12 is placed in the bottle holder 13. The reagent bottle 12 receives the liquid sample transferred from the injection pump 7. The control and display system 24 includes the control display 15, and provides a human-computer interaction interface and automatic control.

[0032] The sample area 21, the reagent area 25, and the array injection pump 22 are arranged in a working area directly below the three-coordinate gantry 23, and the control and display system 24 is arranged on the side of the three-coordinate gantry 23, as shown in Figure 2 .

[0033] Further, in the technical solution, the reagent area 25 can be arranged in the working area directly below the three-coordinate gantry 23, and the sample area 21, the array injection pump 22, and the control and display system 24 can be arranged around the periphery of the three-coordinate gantry 23. The specific positions can be determined according to actual requirements.

[0034] Further, in the technical solution, the sample area 21 and the reagent area 25 can be integrated on one bottle holder tray 14, or can be arranged on two bottle holder trays 14, respectively, as shown in Figure 3 .

[0035] Further, in the technical solution, the sample area 21 and the reagent area 25 are marked with corresponding positions for placing the sample bottle 1 and the reagent bottle 12. The sample bottle 1 and the reagent bottle 12 can be placed in the corresponding positions each time the sample bottle 1 and the reagent bottle 12 are replaced. The number of the sample area 21 and the reagent area 25 can be adjusted according to requirements.

[0036] Further, in the technical solution, the sample bottle 1 can be a single sample bottle 1, or can be an array of multiple types of sample bottles 1, which is convenient for solution processing. The sample bottle 1 can be placed on the bottle holder 13, which is convenient for replacement.

[0037] Further, in the above technical solution, the sample pipeline 3 can be inserted into each sample bottle 1, and the automatic switching between the sample bottles 1 can be realized by opening and closing the two-way medium valve 2 on the sample pipeline 3; or the two-way medium valve 2 can not be provided, and the sample pipeline 3 can be manually switched to realize the switching between the sample bottles 1.

[0038] Further, in the above technical solution, the injection pump 7 can be one injection pump 7 or an array of multiple injection pumps 7. When multiple injection pumps 7 are provided, different specifications of injection pumps 7 can be reasonably arranged according to the range of the transferred liquid, for example, when the range of the transferred liquid is 0.1-100 mL, three specifications of injection pumps 7 of 1 mL, 10 mL and 25 mL can be arranged. The injection pump 7 can be directly installed on the cantilever of the gantry to reduce the length of the pipeline. Further, in the above technical solution, the three-coordinate gantry 23 can be replaced by other mechanical devices such as a mechanical arm to realize the three-coordinate movement.

[0039] Further, in the above technical solution, the reagent bottle 12 can be a single type of reagent bottle 12, or different sizes and types of reagent bottles 12, and the compatibility between the reagent bottles 12 and the bottle holder tray 14 can be realized by replacing the bottle holder 13. Figure 3 Commonly used: conical flask, volumetric flask, test tube, beaker, evaporating dish, test tube, wide-mouth bottle, sample tube or customized container.

[0040] A processing flow of a multi-sample and multi-reagent bottle compatible wide-volume automatic pipetting platform device: 1. Single sample dispensing flow: (1) Preparation: place a single sample in the sample area 21 at a designated position (such as position 1), place a target reagent bottle 12 in the reagent area 25 at a corresponding position, and record the position information.

[0041] (2) Parameter setting: set the specifications and required dispensing volume of each position reagent bottle 12 through the control and display system 24.

[0042] (3) Pipeline cleaning: the three-coordinate gantry 23 moves the pipetting needle 10 to a special emptying position (not shown). The two-way medium valve 2 corresponding to the sample bottle 1 keeps open state, and the injection pump 7 is driven to suck and empty at least one cycle of sample to clean the pipeline and the cavity of the injection pump 7.

[0043] (4) Execute dispensing: the three-coordinate gantry 23 controls the pipetting needle 10 to move above the target reagent bottle 12 (X, Y coordinate positioning). According to the specifications of the reagent bottle 12, the gantry Z axis is lowered to a preset height. The system automatically selects and combines the optimal injection pump 7 range (such as 25 mL and 10 mL injection pumps for 31 mL) according to the set dispensing volume, and efficiently completes the liquid transfer under the premise of ensuring accuracy.

[0044] (5) Sequential execution: if there are two or more reagent bottles 12, repeat step (4) until all reagent bottles 12 are processed.

[0045] 2. Multi-sample multi-reagent bottle dispensing process: (1) Preparation: place multiple samples at different positions in the sample area 21, and place multiple reagent bottles 12 at corresponding positions in the reagent area 25.

[0046] (2) Parameter setting: through the control and display system 24, set three key parameters for each dispensing task: target reagent bottle 12 position and its specifications, dispensing volume, and corresponding sample source position.

[0047] (3) Sequential execution: the control and display system executes according to the set sequence: a. Open the two-way medium valve 2 corresponding to the first sample source.

[0048] b. Perform line cleaning for this sample, same as single-sample dispensing step (3).

[0049] c. Perform dispensing of this sample to all its target reagent bottles 12, same as single-sample dispensing step (4).

[0050] d. Close the two-way medium valve 2 of the current sample source and open the two-way medium valve 2 of the next sample source.

[0051] e. Repeat steps b-d until all samples are processed.

[0052] 3. Solution preparation process: the operation process is basically the same as the multi-sample multi-reagent bottle dispensing process. The core difference is that samples (or reagents) of different sources will be moved into the same target reagent bottle 12, achieving mixing and preparation of solutions.

[0053] Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present application, and are not limited thereto; although the present application has been described in detail with reference to the foregoing embodiments, those skilled in the art should understand that they can still modify the technical solutions described in the foregoing embodiments, or make equivalent substitutions for part or all of the technical features; and these modifications or substitutions do not cause the essence of the corresponding technical solution to deviate from the scope of the technical solutions of the embodiments of the present application.

Claims

1. A multi-sample, multi-reagent bottle compatible wide volume automated pipetting platform apparatus, characterized in that, The sample area, array injection pump, three-coordinate gantry, reagent area, control and display system are included. The sample area is used for placing sample bottles. The array injection pump includes a syringe pump, a sample pipeline, a two-way medium valve, a multi-way joint, a sample suction pipeline and a sample injection pipeline, one end of the sample pipeline is inserted into a sample bottle, the other end is connected to the multi-way joint, the two-way medium valve is arranged on the sample pipeline to open and close the sample pipeline, one end of the sample suction pipeline and the sample injection pipeline is connected to the sample suction joint and the sample injection joint of the syringe pump respectively, the other end of the sample suction pipeline is connected to the multi-way joint, and the other end of the sample injection pipeline is fixed to a suspended pipette tip. The three-coordinate gantry includes a pipette tip and a three-coordinate cylinder gantry, and the pipette tip is installed on the three-coordinate cylinder gantry. The reagent area includes reagent bottles, and the reagent bottles receive liquid samples transferred from the syringe pump. The control and display system includes a control display, and provides a human-computer interaction interface and automatic control.

2. The multi-sample, multi-reagent bottle compatible wide volume automated pipette platform apparatus of claim 1, wherein, The sample area, reagent area and array injection pump are arranged in a working area below the three-coordinate gantry, and the control and display system is arranged on the side of the three-coordinate gantry.

3. The multi-sample, multi-reagent bottle compatible wide volume automated pipette platform apparatus of claim 2, wherein, The reagent area can be arranged only in the working area directly below the three-coordinate gantry, and the sample area, array injection pump and control and display system are arranged around the periphery of the three-coordinate gantry.

4. The multi-sample, multi-reagent bottle compatible wide volume automated pipette platform apparatus of claim 1, wherein, The three-coordinate cylinder gantry is an H-shaped mechanical structure, the pipette tip is installed on the middle beam of the H-shaped structure, and the three-coordinate gantry can realize three-dimensional movement of the pipette tip in x, y and z axes.

5. The multi-sample, multi-reagent bottle compatible wide volume automated pipette platform apparatus of claim 1, wherein, The reagent area further includes bottle holders and a bottle holder tray, the bottle holders are arranged on the bottle holder tray, and the reagent bottles are arranged in the bottle holders.

6. A process flow for a multi-sample, multi-reagent bottle compatible wide volume automated pipetting platform apparatus based on the multi-sample, multi-reagent bottle compatible wide volume automated pipetting platform apparatus of any one of claims 1-5, wherein, The single-sample liquid separation process, multi-sample and multi-reagent bottle liquid separation process and solution preparation process are included.

7. The process flow of a multi-sample, multi-reagent bottle compatible wide volume automated pipette platform apparatus of claim 6, wherein, The single-sample liquid separation process includes the following steps: (1) Preparation: place a single sample in a designated position of the sample area, place a target reagent bottle in a corresponding position of the reagent area, and record the position information; (2) Parameter setting: set the specifications and required separation volume of each position reagent bottle through the control and display system; (3) Pipeline cleaning: the three-coordinate gantry moves the pipette tip to a special emptying position, the two-way medium valve corresponding to the sample bottle keeps open state, and the syringe pump is driven to suck and empty at least one cycle of sample to clean the pipeline and the syringe pump cavity; (4) Execute separation: the three-coordinate gantry controls the pipette tip to move above the target reagent bottle, the gantry Z axis is lowered to a preset height according to the specifications of the reagent bottle, and the control and display system automatically selects and combines the optimal syringe pump range according to the set separation volume, to efficiently complete liquid transfer under the premise of ensuring accuracy; (5) Sequential execution: if there are two or more separation reagent bottles, repeat step (4) until all reagent bottles are processed.

8. The process flow of a multi-sample, multi-reagent bottle compatible wide volume automated pipette platform apparatus of claim 6, wherein, The multi-sample and multi-reagent bottle liquid separation process includes the following steps: (1) Preparation: place multiple samples in different positions of the sample area, and place multiple reagent bottles in corresponding positions of the reagent area; (2) Parameter setting: set three key parameters for each separation task through the control and display system: target reagent bottle position and specifications, separation volume, and corresponding sample source position; (3) Sequential execution: the system executes according to the set sequence: a. open the two-way medium valve corresponding to the first sample; b. execute step (4) of the single-sample liquid separation process; c. close the two-way medium valve corresponding to the first sample; d. open the two-way medium valve corresponding to the second sample; e. execute step (4) of the single-sample liquid separation process; f. close the two-way medium valve corresponding to the second sample; g. repeat the above steps until all samples are processed. b. Perform line cleaning for the sample; c. Perform dispensing of the sample to all its target reagent vials; d. Close the two-way media valve of the current sample source and open the two-way media valve of the next sample source; e. Repeat steps b-d until all samples are processed.

9. The process flow of a multi-sample, multi-reagent bottle compatible wide volume automated pipette platform apparatus of claim 6, wherein, Wherein the solution preparation procedure comprises the following steps: (1) Preparation: Place multiple samples at different positions in the sample area and multiple reagent vials at corresponding positions in the reagent area; (2) Parameter setting: Through the control and display system, set three key parameters for each dispensing task: target reagent vial position and its specification, dispensing volume, and corresponding sample source position; (3) Sequential execution: The system executes according to the set sequence: a. Open the two-way media valve corresponding to the first sample; b. Perform line cleaning for the sample; c. Perform dispensing of the sample to all its target reagent vials; d. Close the two-way media valve of the current sample source and open the two-way media valve of the next sample source; e. Repeat steps b-d until all samples are transferred into the same target reagent vial, achieving mixing and preparation of the solution.

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