A gel controllable separation and purification device and method

Abstract

The application discloses a gel controllable separation and purification device and method, which comprises a feed liquid tank unit, a gel special separation and purification protection cylinder, a gel controllable separation and purification device controller, a cooling and slow flow unit and a membrane separation unit. The protection cylinder can make various shapes of gel materials always suspended in the area of the central axis to do circular motion, thereby ensuring the stability of the rheological characteristics and functional activity of the gel. In view of the fact that the gel separation and purification device special for gel materials has not been sold on the market, and different from the uncontrollable or random method that the gel preparation reaction is determined to be completed after the gel formation is observed by naked eyes and the gel is simply washed or soaked in water for multiple times, the device and method can make up the short board that the gel material preparation reaction cannot be effectively controlled in the past, realize the technical controllability and process repeatability which have not been achieved in the past, and solve the technical problems of the stability and uniformity of the gel material.

Description

Technical Field

[0001] This invention relates to the field of separation and purification equipment technology, and more specifically, to an equipment specifically suitable for desalting and removing impurities from macromolecular gel materials and for effective quality control of gel preparation reactions. Background Technology

[0002] A gel is a colloidal dispersion system consisting of a three-dimensional network solid framework and a liquid medium filling its voids. The gel framework, or gelling agent, forms a continuous three-dimensional network structure, providing solid-state strength and elasticity; the gel liquid, or dispersion medium, is encapsulated within the framework and cannot flow freely, thus giving the gel its wet and soft properties. Due to its unique structure, a gel appears as a non-flowing, soft solid, but its internal components are mostly liquid.

[0003] In daily life, foods such as jelly, pudding, yogurt, gummies, tofu, ice cream, and meat jelly, daily necessities such as toothpaste, shampoo, shower gel, styling gel, face masks, and diapers, as well as medical coupling agents used in ultrasound examinations, ointments, stationery glue, and silica gel in desiccants all fall under the category of gels.

[0004] In science and industry, gel chromatography using gels as packing materials can be used to separate molecules of different sizes; agarose gels or polyacrylamide gels are used to separate DNA, RNA, or proteins; in tissue engineering, hydrogels serve as scaffolds for cell growth and repair; and in drug delivery, gels act as drug carriers, enabling controlled release. Furthermore, some gels can respond to external stimuli (such as pH, temperature, and light), changing their volume and possessing great potential as sensors and smart materials.

[0005] One of the most widely researched and applied types of gels is the hydrogel, which uses water as the dispersion medium. Hydrogels are hydrophilic three-dimensional network structures that swell rapidly in water and retain a large volume of water without dissolving. Their unique physical properties provide novel solutions for modern pharmacy and medicine, ranging from drug delivery systems and tissue engineering to wound dressings and biosensors. An upgraded version of hydrogels is the emulsion gel, a soft solid formed by uniformly dispersing emulsified droplets within a gel matrix. It combines the advantages of both emulsions and gel networks, exhibiting structural stability and functional diversity. The shape of gel materials is not fixed; their diversity depends primarily on their preparation methods and application requirements. Examples include common bulk gels and microspheres / nanospheres.

[0006] To date, domestic and international literature and patents concerning gel material preparation generally rely on visual observation of gel formation (clumps, spheres, etc.), followed by simple rinsing with water or repeated soaking with different water to subjectively determine the completion of the gel preparation reaction. It is widely known that gelation is a complex chemical reaction, involving not only the complete construction of the gel's three-dimensional network structure but also the effective encapsulation of active substances, drugs, and even cells. How can one subjectively determine the completion of the gel preparation reaction using a combination of visual observation and random impurity removal? This method is both inefficient—consuming a significant amount of time—and uncontrollable—lacking a means of detection to determine whether the gelation reaction is complete. Gel materials prepared in this way obviously lack homogeneity, and there is no batch-to-batch stability. It is well known that chemical preparation processes, in addition to the synthesis and polymerization of compounds / polymers, also include crucial separation and purification. However, current domestic and international patent literature has not published / disclosed effective quality control techniques for gel preparation reactions, meaning that most current gel preparation reaction processes are likely incomplete.

[0007] Meanwhile, due to the weak mechanical strength of gels and the fact that their rheological properties are easily affected by temperature changes, there is currently no device available on the market that can be used for large-scale separation and purification of gels.

[0008] Furthermore, if some gel materials need to be stably preserved in cell culture medium or under specific pH / salt conditions, large amounts of cell culture medium or pH / salt buffer solution must be used for washing or soaking, which will result in a huge waste of cell culture medium or buffer solution.

[0009] Therefore, there is an urgent need to develop a reliable device specifically designed for substances like gels, which have relatively weak inherent mechanical strength, to quickly remove impurities and desalts and achieve effective quality control of the gel preparation reaction. Summary of the Invention

[0010] The purpose of this invention is to provide a controllable gel separation and purification apparatus and method to solve at least one of the problems mentioned in the background art.

[0011] To achieve the above objectives, the present invention provides the following technical solution:

[0012] A gel controllable separation and purification device includes a feed tank unit, a gel-specific separation and purification protective cylinder (67), a gel controllable separation and purification device controller (8), a cooling and slow flow unit, and a membrane separation unit;

[0013] The liquid tank unit includes a liquid tank (16) and a liquid tank temperature-controlled cooling circulation pump (3) that provides cooling for it. The liquid tank temperature-controlled cooling circulation pump (3) is connected to the liquid tank temperature-controlled condensate inlet (29) and liquid tank temperature-controlled condensate return outlet (23) set on the liquid tank (16) through the liquid tank temperature-controlled cooling circulation pump condensate output pipeline (14) and the liquid tank temperature-controlled cooling circulation pump condensate return pipeline (13) to form a cooling circulation loop. It also includes a conductivity detector, which is used to detect the conductivity of the permeate flowing out of the permeate outlet and the liquid flowing into the liquid tank inlet.

[0014] The gel-specific separation and purification protective tube (67) is fixedly installed inside the liquid tank (16) by the protective tube support rod (65) and the protective tube fixing column foot (70);

[0015] The membrane separation unit is connected to the outlet at the bottom of the feed tank (16) through the membrane separation system pipeline (44). The membrane separation system pipeline (44) is provided with a feed pump (61), a pressure pump (62), a filter barrel (59) and a membrane tube (57) in sequence along the material flow direction.

[0016] The cooling and slowing flow unit is connected between the output end of the membrane separation unit and the inlet end of the feed tank (16); the cooling and slowing flow unit includes a cooling and slowing flow device (41) and a cooling and cooling circulation pump (48) that provides cooling for it. The cooling and cooling circulation pump (48) is connected to the cooling and slowing flow device (41) through a cooling and cooling circulation pump condensate output pipeline (56) and a cooling and cooling circulation pump condensate return pipeline (50) to form a cooling circulation loop; the inlet of the cooling and slowing flow device (41) is connected to the return end of the membrane separation unit through a pipeline, and its outlet is connected to the cooling and slowing flow liquid inlet (24) on the feed tank (16) through a pipeline.

[0017] The controller (8) of the gel controllable separation and purification device is electrically connected to the temperature control cooling circulation pump (3) of the feed tank, the monitoring and control elements on the feed tank (16), the cooling circulation pump (48), the control valve in the cooling slow flow unit, the feed pump (61) in the membrane separation unit, the pressurization pump (62) and the reflux control valve through multiple electrical signal transmission lines, and is used to control the operating parameters of the entire device.

[0018] Furthermore, the gel-specific separation and purification protective tube (67) includes a cylindrical protective tube wall (68), a solid protective tube bottom (69) with a closed bottom, a protective tube support rod (65) located on the upper edge of the protective tube wall (68), and a protective tube fixing column foot (70) located on the bottom; the protective tube wall (68) is densely covered with small holes that are obliquely cut upward from the outside of the tube to the inside of the tube; the upper edge of the protective tube wall (68) is also inlaid with a protective tube angular velocity monitoring probe (66) with a built-in WIFI signal transmitting device.

[0019] Furthermore, the controller (8) of the gel controllable separation and purification device has a built-in WIFI signal receiver for receiving the signal emitted by the protective cylinder angular velocity monitoring probe (66); the surface of the controller (8) of the gel controllable separation and purification device is provided with a device controller panel (7), which is a touch screen for parameter setting and device control.

[0020] Furthermore, the top of the liquid tank (16) is provided with an openable liquid tank cover (20) and a liquid tank inlet (12), and the liquid tank inlet (12) is provided with a liquid tank inlet control valve (19); the side wall of the liquid tank (16) is provided with a water level gauge (27) and a liquid tank temperature monitoring probe (28); the bottom of the liquid tank (16) is provided with a liquid tank horizontal base (30), the liquid tank horizontal base (30) is provided with a drain hole, and a discharge port is provided below it, the discharge port is provided with a liquid tank discharge port control valve (33) and a liquid tank discharge port control valve (32); the cooling slow-flow liquid inlet (24) is provided on the side wall of the liquid tank (16), and its insertion direction is the tangent direction of the horizontal cross-sectional circle.

[0021] Furthermore, the cooling and slowing flow unit also includes a cooling and slowing flow liquid three-way valve (37) and a cooling and slowing flow temperature monitoring probe (38) installed in the pipeline; the cooling and slowing flow device (41) is provided with a layered and tortuous cooling and slowing flow coil (40) inside, and the liquid to be cooled flows through the cooling and slowing flow coil (40).

[0022] Furthermore, the membrane tube (57) of the membrane separation unit is provided with a permeate outlet (58) of the membrane separation system, and the end pipeline is provided with a reflux control valve (60) of the membrane separation system.

[0023] Furthermore, the controller (8) of the gel controllable separation and purification device controls the temperature inside the feed tank (16) via the feed tank temperature control electrical signal transmission line (5) and the feed tank temperature monitoring electrical signal transmission line (18); controls the temperature of the reflux liquid via the cooling circulating pump temperature control electrical signal transmission line (11) and the cooling slow-flow liquid temperature monitoring electrical signal transmission line (45); and controls the liquid level inside the feed tank (16) via the feed tank liquid inlet control valve electrical signal transmission line (17) and the water level sensor electrical signal transmission line (36). The flow rate of the liquid entering the feed tank (16) is controlled by the three-way valve of the cooling and slow-flow liquid control line (46) and the angular velocity signal received is used to control the angular velocity in the gel-specific separation and purification protective tube (67); the pressure in the membrane tube (57) is controlled by the reflux control valve of the membrane separation system control line (54); the start and stop of the feed pump (61) and the pressurization pump (62) are controlled by the feed pump control line (63) and the pressurization pump control line (64) respectively.

[0024] Furthermore, the membrane tube (57) in the membrane separation unit can be adapted to spiral wound membranes, flat sheet membranes, or electrodialysis membrane separation systems. The present invention also provides a method for gel separation and purification using the aforementioned controllable gel separation and purification device, characterized by comprising the following steps:

[0025] S1. Preparation and initialization steps: Fix the gel-specific separation and purification protective tube (67) inside the liquid tank (16), close the liquid tank discharge port control valve (32) and the liquid tank outlet control valve (33); inject liquid into the liquid tank (16) through the liquid inlet (12) until the liquid level monitored by the water level gauge (27) reaches the preset height of the gel controllable separation and purification device controller (8);

[0026] S2. Temperature control steps: Start the temperature control cooling circulation pump (3) and the cooling circulation pump (48) of the liquid tank, so that the temperature of the liquid in the liquid tank (16) is reduced to and maintained at the preset low temperature by the circulating condensate of the cooling jacket (25), while the cooling buffer (41) is in standby cooling state.

[0027] S3. Membrane separation system start-up steps: Open the feed tank outlet control valve (33), start the feed pump (61) and pressurization pump (62) in sequence, so that the feed liquid flows through the membrane separation system pipeline (44) and filter tank (59) into the membrane tube (57); adjust the membrane separation system reflux control valve (60) to stabilize the internal pressure of the membrane tube (57) at the preset value;

[0028] S4. Circulation and Suspension Control Steps: The concentrated reflux liquid separated by the membrane tube (57) flows into the cooling and slowing flow device (41) and is rapidly cooled. Then, it enters the feed tank (16) tangentially through the cooling and slowing flow liquid inlet (24). The controller (8) of the gel controllable separation and purification device receives the real-time angular velocity signal from the protective cylinder angular velocity monitoring probe (66) and adjusts the liquid flow rate entering the feed tank (16) by regulating the cooling and slowing flow liquid three-way valve (37), so that the liquid in the gel-specific separation and purification protective cylinder (67) generates circumferential motion and reaches the preset angular velocity. Then, the gel is added to ensure that the added gel material can be suspended in the area near the central axis of the protective cylinder.

[0029] S5. Endpoint Judgment and Collection Steps: Real-time detection of the conductivity of the liquid flowing out of the permeate outlet (58) of the membrane separation system. When the conductivity value reaches the preset endpoint, the gel separation and purification is determined to be complete, and the purified gel product is collected from the gel-specific separation and purification protective tube (67).

[0030] Furthermore, in the circulation and suspension control step, the controller (8) of the gel controllable separation and purification device regulates the angular velocity according to the following principle: if the real-time angular velocity is lower than the preset value, the liquid flow rate into the feed tank (16) is increased; if the real-time angular velocity is higher than the preset value, the liquid flow rate into the feed tank (16) is reduced, and part of the liquid is diverted through the cooling slow flow liquid three-way valve (37) and returned to the membrane separation system pipeline (44) through the cooling slow flow liquid return pipeline (43).

[0031] This invention also provides an application of the aforementioned controllable separation and purification device for preparing gel products; wherein, the application includes using the device to perform large-scale desalting and impurity removal of gel materials and to achieve effective quality control of the gel preparation reaction, and the gel product is selected from any of the following fields:

[0032] Biomedical materials: gel-based drug delivery systems for controlled drug release, hydrogels for tissue engineering scaffolds, medical wound dressings, or biosensor interface materials;

[0033] Biochemical separation media: gel packing materials for gel chromatography, agarose gels or polyacrylamide gels for separating DNA, RNA or proteins;

[0034] Gel core ingredients in food industry products (jelly, pudding, yogurt, gummy or meat jelly) and daily chemical products (toothpaste, shampoo, shower gel, styling gel or face mask).

[0035] As a further aspect of the present invention: the membrane separation unit is a membrane separation system suitable for spiral wound membranes (e.g., Figure 1As shown, it can be modified to a membrane separation system suitable for flat sheet membranes or an electrodialysis membrane separation system, depending on actual needs. Both can achieve the ideal effect of desalting and removing impurities from gel materials and effectively controlling the quality of gel preparation reactions.

[0036] Compared with the prior art, the beneficial effects of the present invention are:

[0037] 1. Based on the fact that previous domestic and foreign patent literature has not published / disclosed technical solutions for effective quality control of gel preparation reactions, and that there is currently no commercially available device for desalting and removing impurities from gel materials, this invention provides a novel, highly efficient and controllable device specifically designed for the large-scale separation and purification of gels with weak inherent mechanical strength. This solves the long-standing problem in domestic and foreign literature and patents regarding the equipment for efficient desalting and removing impurities from gel materials and effective quality control of gel preparation reactions.

[0038] 2. The present invention provides a controllable separation and purification device for gels. The dedicated separation and purification protective tube for gels can perfectly protect the gels, protecting them from the impact of high-speed water flow in the device pipeline, the mechanical squeezing of the feed pump and pressurization pump in the device pipeline, and the shearing collision and hydrophilic adsorption between the gel and the separation medium (such as spiral wound membrane core or flat membrane sheet) in the membrane tube, thereby ensuring the stability of the rheological characteristics and functional activity of the gels.

[0039] 3. The present invention provides a controllable separation and purification device for gels, wherein the opening direction of the small hole of the gel-specific separation and purification protective cylinder is designed to be inclined upward at 45° from the outside to the inside, thereby dividing the water flow impact force in the feed tank of the device into an equal upward force and a centripetal force (e.g., Figure 3 As shown); where the upward force is used to counteract the gravity of the gel, preventing it from sinking to the bottom of the gel separation and purification protective tube; on this basis, the centripetal force keeps the gel suspended in a circular motion near the central axis region of the gel separation and purification protective tube (as shown). Figure 4 As shown in the figure, this avoids contact between the gel and the wall of the gel-specific separation and purification protective cylinder, which is full of small pores, thus protecting the integrity of the gel material.

[0040] 4. The present invention provides a controllable separation and purification device for gels. The movement of the gel within the dedicated gel separation and purification protective cylinder follows a centripetal force F = mω²r (where m is the weight of the object; ω is the angular velocity of the object's circular motion; and r is the radius of the object's motion). Under the condition that the angular velocity (ω) and the radius of motion (r) are the same, the weight of the gel is much greater than the weight of the small molecule impurities and salts that need to be removed. Therefore, the small molecule impurities and salts can be easily "flyed out" from the gel system, thereby achieving the ideal effect of rapid separation and purification (e.g., Figure 4(As shown). Therefore, compared with the inefficient and subjective methods of desalination and impurity removal commonly used in previous literature, such as rinsing with clean water or soaking in different water, the present invention has significant progress.

[0041] 5. The present invention provides a controllable separation and purification device for gels, wherein the gel-specific separation and purification protective tube is made of environmentally friendly and hydrophobic plastic material (PC, food-grade polycarbonate, the material of water barrels), which not only ensures the sturdiness and durability of the specially made gel-specific separation and purification protective tube, but also avoids unnecessary interference and adsorption caused by the gel coming into contact with the inner wall of the protective tube.

[0042] 6. The present invention provides a controllable gel separation and purification device, which features a specially designed complete cooling and slow-flow unit, including a three-way valve for cooling and slow-flow liquid, a cooling and slow-flow temperature monitoring probe, and a cooling and slow-flow device (such as...). Figure 6 As shown), the cooling and slow-flow liquid return pipeline and the cooling circulation pump; the hot liquid returning after purification by the membrane core in the membrane tube passes through the stacked and tortuous cooling and slow-flow coils in the cooling and slow-flow device, which can cause the liquid temperature to drop rapidly. If the liquid temperature reaches the feed liquid temperature set by the main controller, the cooling and slow-flow liquid three-way valve will be automatically closed, allowing the liquid to enter the feed liquid tank; if the liquid temperature is higher than the feed liquid temperature set by the main controller, the cooling and slow-flow liquid three-way valve will be automatically opened, allowing the liquid to return to the membrane separation system pipeline through the cooling and slow-flow liquid return pipeline, and the cooling power of the cooling circulation pump will be increased. Furthermore, the cooling and flow-slowing device of this invention draws inspiration from the successful example of the ancient Chinese Lingqu Canal project, which "transformed straight lines into curves to slow the water flow." Its layered and convoluted coil structure effectively reduces the flow velocity of the turbulent liquid after recirculation through the membrane core. This ensures the gel obtains sufficient angular velocity (ω) to achieve circular motion while suspended in a specially designed gel-specific separation and purification protective cylinder, thus achieving rapid separation of the gel from small molecule impurities and salts. It also avoids excessive centripetal force generated by the gel due to excessive angular velocity (in this invention, centripetal force = upward lifting force, see...). Figure 3 This upward force exceeds the gel's gravity, causing the gel to jump directly out of the gel-specific separation and purification protective cylinder, which is unfavorable. Therefore, if the liquid flow rate is sufficient to bring the liquid in the gel-specific separation and purification protective cylinder to the angular velocity set by the controller of the controllable separation and purification device, the cooling and slow-flowing three-way valve will automatically close, allowing the liquid to enter the feed tank. If the liquid flow rate exceeds the angular velocity set by the controller of the controllable separation and purification device, the cooling and slow-flowing three-way valve will automatically adjust, allowing some of the liquid to return to the membrane separation system pipeline, while the remaining liquid can enter the feed tank, bringing the liquid in the gel-specific separation and purification protective cylinder to the angular velocity set by the controller of the controllable separation and purification device.

[0043] 7. The gel controllable separation and purification device provided by this invention has a specially designed insertion direction of the cooling and slow-flowing liquid inlet (24) into the feed tank, which is tangential to the cross-sectional circle of the cylindrical body at the level of the inlet. This allows the liquid in the feed tank to move in a circular motion, which in turn drives the liquid in the gel-specific separation and purification protective cylinder to move in a circular motion. Therefore, the device of this invention does not require an additional stirring motor and stirring paddle or other magnetic stirring device, which saves equipment costs and avoids the adverse phenomenon of the stirring paddle hitting the weak gel material, causing it to break down. Therefore, this invention has a certain degree of innovation.

[0044] 8. The present invention provides a controllable separation and purification device for gels. One side of the device, the feed tank, is maintained at a consistently low temperature to protect heat-sensitive gel materials from the effects of temperature changes (e.g., changes in gel rheological properties or helical denaturation of macromolecular proteins). The other side, the membrane tube, is maintained at a relatively high temperature to ensure that the separation medium (e.g., a spiral wound membrane core or a flat sheet membrane) effectively separates small molecule impurities and salts that are "swept away" from the gel system. Therefore, the device of the present invention is rationally designed, with each system component performing its specific function and achieving good results, thus greatly expanding the applicability of the device.

[0045] 9. The present invention provides a gel controllable separation and purification device, which is specially designed with two cooling circulation pumps—a feed tank temperature-controlled cooling circulation pump and a cooling circulation pump; the feed tank temperature-controlled cooling circulation pump is used to ensure that the large volume of liquid in the feed tank is always kept at a low temperature, while the cooling circulation pump is used to quickly cool the hot liquid after it is returned through the membrane core. The two cooling circulation pumps are used for different cooling objects and have different cooling powers. The use of the two pumps separately can enable the device of the present invention to achieve the best effect.

[0046] 10. The present invention provides a controllable separation and purification device for gels. Since most gels are hydrophilic gels, they need to be washed and stored with deionized water. Therefore, when the permeate flowing out of the permeate outlet of the membrane separation system is measured to have an extremely low conductivity (e.g., when the gel material is used in the field of biomedical materials or biochemical separation media, the conductivity is ≤0.0001S / m; when the gel material is used in the field of food industry, the conductivity is ≤0.001S / m; when the gel material is used in the field of daily chemical products, the conductivity is ≤0.01S / m), it can be determined that the preparation reaction of the gel material in the gel-specific separation and purification protective tube has been completed. If certain gel materials require specific cell culture media or pH conditions for stable preservation, these media or pH buffers will have a certain conductivity. When the conductivity of the permeate flowing from the permeate outlet of the membrane separation system is measured to be close to that of the liquid flowing into the feed tank (e.g., conductivity similarity ≥ 99.99% for gel materials used in biomedical materials or biochemical separation media; ≥ 99.00% for gel materials used in the food industry; ≥ 95.00% for gel materials used in daily chemical products), it can be determined that the preparation reaction of the gel material in the dedicated gel separation and purification protective cartridge has been completed. Given the uncontrollable or random methods of water washing or soaking for desalination and impurity removal commonly used in previous literature, the apparatus and method of this invention can achieve technical controllability, process repeatability, and the integrity of the preparation reaction that are unprecedented in previous literature. The "conductivity similarity" mentioned in this invention refers to the percentage of the conductivity of the liquid flowing into the feed tank inlet to the conductivity of the permeate flowing out of the membrane separation system outlet.

[0047] 11. The present invention provides a controllable separation and purification device for gels, specifically addressing situations where certain gel materials require specific cell culture media or pH / salt conditions for stable preservation. If large amounts of cell culture media or pH / salt buffer solutions are still used for washing or soaking, it results in a significant waste of expensive cell culture media or buffer solutions. The membrane separation unit of the present invention can rapidly remove small molecule impurities and salts, and also allows most of the cell culture media or pH / salt buffer solution to be returned to the feed tank, avoiding waste. Therefore, the present invention represents a significant advancement.

[0048] 12. This invention provides a controllable gel separation and purification device. The cooling and slow-flow unit located in the middle integrates the gel purification equipment (feed tank unit + gel-specific separation and purification protective cylinder) and the desalting and impurity removal equipment (membrane separation unit) into a complete production unit. The entire device has a novel and compact structure, effectively reducing the footprint. Furthermore, this invention is simple and convenient to operate; only one device controller is needed to control the entire gel purification and desalting / impurity removal process. Only one technician is required to operate and control the device, minimizing labor costs. Attached Figure Description

[0049] The present invention will be further described below with reference to the accompanying drawings and embodiments.

[0050] Figure 1 This is a schematic diagram of the structure of a gel-controlled separation and purification device according to the present invention.

[0051] Figure 2 A schematic diagram of the structure of a protective cartridge for gel separation and purification.

[0052] Figure 3 This is a schematic diagram showing water flowing through the small holes in the protective cylinder wall of a gel-specific separation and purification system.

[0053] Figure 4 This is a schematic diagram of the gel undergoing circular motion within a dedicated gel separation and purification protective tube.

[0054] Figure 5 This is a schematic diagram of the device's controller panel.

[0055] Figure 6 This is a schematic diagram of a cooling flow retarder.

[0056] In the diagram: 1. Display screen for the temperature-controlled cooling circulation pump of the feed tank; 2. Switch for the temperature-controlled cooling circulation pump of the feed tank; 3. Temperature-controlled cooling circulation pump of the feed tank; 4. Heat dissipation baffle for the temperature-controlled cooling circulation pump of the feed tank; 5. Electrical signal transmission line for temperature control of the feed tank; 6. Brake pulley for the temperature-controlled cooling circulation pump of the feed tank; 7. Device controller panel; 8. Controller for the gel controllable separation and purification device; 9. Operating indicator light for the device controller; 10. Switch for the device controller; 11. Electrical signal transmission line for temperature control of the cooling circulation pump; 12. Liquid inlet of the feed tank; 13. Condensate return pipeline of the temperature-controlled cooling circulation pump of the feed tank; 14. Condensate output pipeline of the temperature-controlled cooling circulation pump of the feed tank; 15. Brake pulley for the device controller; 16. 17. Liquid tank; 18. Liquid tank inlet control valve electrical signal transmission line; 19. Liquid tank temperature monitoring electrical signal transmission line; 20. Liquid tank inlet control valve; 21. Openable liquid tank cover; 22. Glass window; 23. Water level gauge upper inlet; 24. Liquid tank temperature control condensate return port; 25. Cooling slow-flow liquid inlet; 26. Cooling jacket; 27. Water level gauge upper and lower inlet valves; 28. Water level gauge; 29. ​​Liquid tank temperature monitoring probe; 30. Liquid tank temperature control condensate inlet; 31. Liquid tank horizontal base; 32. Water level gauge lower inlet; 33. Liquid tank discharge port control valve; 34. Liquid tank outlet control valve; 35. Liquid tank brake pulley; 36. Liquid tank column base; 37. Water level sensor electrical signal transmission line; 38. Cooling slow-flow liquid three-way valve; 39. Cooling slow-flow temperature monitoring probe; 40. Cooling slow-flow outlet; 41. Cooling slow-flow coil; 42. Cooling slow-flow device; 43. Cooling circulating pump condensate inlet; 44. Cooling slow-flow liquid return pipeline; 45. Membrane separation system pipeline; 46. Cooling slow-flow liquid temperature monitoring electrical signal transmission line; 47. Cooling slow-flow liquid three-way valve control electrical signal transmission line; 48. Cooling circulating pump display screen; 49. Cooling circulating pump; 50. Cooling circulating pump condensate return pipeline; 51. Cooling circulating pump brake pulley; 52. 53. Cooling circulation pump condensate return port; 54. Cooling buffer inlet; 55. Membrane separation system reflux control valve electrical signal transmission line; 56. Cooling circulation pump switch; 57. Cooling circulation pump condensate output pipeline; 58. Membrane tubing; 59. Membrane separation system permeate outlet; 60. Filter cartridge; 61. Membrane separation system reflux control valve; 62. Feed pump; 63. Pressurization pump; 64. Feed pump control electrical signal transmission line; 65. Pressurization pump control electrical signal transmission line; 66. Protective cylinder support rod; 67. Protective cylinder angular velocity monitoring probe; 68. Gel-specific separation and purification protective cylinder; 69. Protective cylinder wall; 70. Protective cylinder solid bottom; 71. Protective cylinder fixing column foot. Detailed Implementation

[0057] The following embodiments will describe the present invention in detail with reference to the accompanying drawings. In practical applications, the shape, thickness, or height of each component may be enlarged or reduced. The embodiments listed in this invention are only for illustrating the invention and are not intended to limit the scope of the invention. Any obvious modifications or changes made to the invention do not depart from the spirit and scope of the invention.

[0058] Example 1

[0059] Please see Figure 1 and Figure 2 A controllable separation and purification device for gel includes a feed tank unit, a gel-specific separation and purification protective cylinder 67, a controllable separation and purification device controller 8, a cooling and slow-flow unit, and a membrane separation unit.

[0060] The gel-specific separation and purification protective cylinder 67 is located inside the feed tank unit; the cooling and slow-flow unit and the membrane separation unit are located outside the feed tank unit and are connected to the feed tank unit through pipelines; the gel controllable separation and purification device controller 8 is used to control the operation of each unit.

[0061] The gel-specific separation and purification protective tube 67 includes a protective tube support rod 65, a protective tube angular velocity monitoring probe 66, a protective tube wall 68, a solid bottom of the protective tube 69, and a protective tube fixing column foot 70 (e.g., Figure 2 (As shown). The protective cylinder wall 68 is cylindrical. Four protective cylinder support rods 65 are located at the upper end of the protective cylinder wall 68, spaced 90 degrees apart, and arranged radially in the horizontal direction. The protective cylinder angular velocity monitoring probe 66 is also located at the upper end of the protective cylinder wall 68. A solid bottom 69 is located at the bottom of the protective cylinder wall 68 to close the lower opening of the cylinder. Protective cylinder fixing feet 70 are located at the edge of the bottom end of the protective cylinder wall 68 to support it.

[0062] The gel separation and purification protective cartridge 67 is made of environmentally friendly and hydrophobic plastic material (PC, food-grade polycarbonate, the material used in water jugs). The cartridge wall 68 has a thickness capable of withstanding the water pressure inside the cartridge and is densely covered with small pores. The pores are angled upwards at 45° from the outside to the inside of the cartridge, so that the impact force of the water flow entering the cartridge is evenly distributed into the centripetal force and upward force of the gel (e.g., ...). Figure 3(As shown). The protective cylinder angular velocity monitoring probe 66 has a built-in WIFI signal transmitter, its outer layer is wrapped with waterproof material, and it is embedded in the upper edge of the protective cylinder wall 68 which is covered with small holes. During the use of the device, the entire gel-specific separation and purification protective cylinder 67 must be placed inside the liquid tank 16 and fixed inside the liquid tank 16 by the protective cylinder support rod 65 and the protective cylinder fixing column 70. The diameter of the gel-specific separation and purification protective cylinder 67 plus the length of the two oppositely arranged protective cylinder support rods 65 must be equal to the diameter of the inner wall of the liquid tank 16. The height of the gel-specific separation and purification protective cylinder 67 is slightly lower than the height of the liquid tank horizontal base 30 to the opening of the liquid tank 16.

[0063] See Figure 1 The liquid tank unit comprises a liquid tank temperature-controlled cooling circulation pump 3, a liquid tank 16, a liquid tank temperature-controlled cooling circulation pump condensate return pipeline 13, and a liquid tank temperature-controlled cooling circulation pump condensate output pipeline 14. The liquid tank temperature-controlled cooling circulation pump 3 includes a liquid tank temperature-controlled cooling circulation pump display screen 1, a liquid tank temperature-controlled cooling circulation pump switch 2, a liquid tank temperature-controlled cooling circulation pump heat dissipation baffle 4, and a liquid tank temperature-controlled cooling circulation pump brake pulley 6. The liquid tank 16 has an openable cover 20 on top, which has a liquid inlet 12, a liquid inlet control valve 19, and a glass window 21. The side of the liquid tank 16 has a water level gauge inlet 22, a temperature-controlled condensate return port 23, a cooling slow-flow liquid inlet 24, a cooling jacket 25, a water level gauge upper and lower inlet valves 26, a water level gauge 27, a liquid tank temperature monitoring probe 28, and a liquid tank temperature-controlled condensate inlet 29. The bottom of the liquid tank 16 has a horizontal base 30 and a water level gauge lower inlet 31. The bottom of the horizontal base 30 has a discharge port, a liquid tank discharge port control valve 33, a liquid tank discharge port control valve 32, and a discharge port connection to a discharge pipe. The bottom of the liquid tank 16 has a downward-facing liquid tank support 35, and a liquid tank brake pulley 34 at the lower end of the support 35. The temperature-controlled cooling circulation pump 3 of the liquid tank is connected to the liquid tank 16 through the condensate return pipeline 13 and the condensate output pipeline 14 to form a cooling liquid circulation system. The water level gauge 27, equipped with a water level sensor, monitors the water level in the liquid tank 16. The horizontal base 30 of the liquid tank is covered with drain holes for draining the liquid from the liquid tank 16. The cooling and slow-flowing liquid inlet 24 is specifically designed to be inserted into the liquid tank 16 at the tangent to the cross-sectional circle of the cylinder at the inlet level.

[0064] The discharge pipe at the bottom of the feed tank 16 is divided into two paths. One path enters the membrane separation system through the membrane separation system pipeline 44, and the other path connects to the cooling and slow-flowing liquid three-way valve 37 through the cooling and slow-flowing liquid return pipeline 43. The second path of the cooling and slow-flowing liquid three-way valve 37 is connected to the feed tank, and the other path is connected to the cooling and slow-flowing liquid outlet 39 of the cooling and slow-flowing liquid 41.

[0065] See Figure 1 The controllable gel separation and purification device controller 8 includes a device controller panel 7, a device controller operation indicator light 9, a device controller switch 10, and a device controller brake pulley 15. The controllable gel separation and purification device controller 8 has a built-in WIFI signal receiver for receiving the angular velocity WIFI signal emitted by the protective cylinder angular velocity monitoring probe 66 in real time.

[0066] See Figure 1 and Figure 6 The cooling and slowing flow unit consists of a three-way valve 37 for cooling and slowing flow liquid, a temperature monitoring probe 38 for cooling and slowing flow liquid, a cooling and slowing flow device 41, a cooling and slowing flow liquid return pipeline 43, and a cooling and cooling circulation pump 48. The cooling and slowing flow device 41 includes a cooling and slowing flow device outlet 39 (connected to the upper middle part of the liquid tank 16), a cooling and slowing flow coil 40 (composed of multiple S-shaped sections connected end-to-end), a cooling and cooling circulation pump condensate inlet 42 (connected to the cooling and cooling circulation pump 48), a cooling and cooling circulation pump condensate return port 52 (connected to the cooling and slowing flow coil 40), and a cooling and slowing flow device inlet 53 (connected to the membrane separation unit).

[0067] The cooling circulation pump 48 includes a cooling circulation pump display screen 47, a cooling circulation pump heat dissipation baffle 49, a cooling circulation pump switch 55, and a cooling circulation pump brake pulley 51. The cooling circulation pump 48 is connected to the cooling buffer 41 through the cooling circulation pump condensate return pipe 50 and the cooling circulation pump condensate output pipe 56 to form an external circulation; thereby exchanging heat with the fluid (membrane separation system - cooling buffer 41 - feed tank) flowing through the cooling buffer 41.

[0068] See Figure 1 The membrane separation unit includes, in sequence, a feed pump 61, a pressurizing pump 62, a filter cartridge 59, and a membrane tube 57. The feed pump 61 is connected to the discharge pipe at the bottom of the feed tank 16 through the membrane separation system pipeline 44; the front end of the membrane tube 57 is also provided with a membrane separation system permeate outlet 58; the rear end of the membrane tube 57 is provided with a membrane separation system reflux control valve 60.

[0069] The filter cartridge 59 has a built-in filter screen, and the membrane tube 57 has a built-in membrane core. The permeate molecular weight of the membrane core can be selected according to the molecular weight of the monomer raw material of the gel material.

[0070] See Figure 1The controller 8 of the gel controllable separation and purification device controls the start and stop of the cooling circulation pump 3 of the feed tank via the feed tank temperature control electrical signal transmission line 5, and, in conjunction with the feed tank temperature monitoring electrical signal transmission line 18, controls the temperature in the feed tank to always maintain a suitable low temperature condition for gel stability. The controller 8 controls the start and stop of the cooling circulation pump 48 via the cooling circulation pump temperature control electrical signal transmission line 11, and, in conjunction with the cooling slow-flow liquid temperature monitoring electrical signal transmission line 45, rapidly cools the hot liquid returning after purification by the inner spiral membrane core, bringing its temperature to the feed temperature set by the controller 8. The controller 8 controls the start and stop of the feed tank liquid inlet control valve 19 via the feed tank liquid inlet control valve electrical signal transmission line 17, and, in conjunction with the water level sensor electrical signal transmission line 36, controls the liquid level in the feed tank to always reach the liquid level set by the controller 8. The controller 8 of the gel controllable separation and purification device adjusts the flow rate of the cooling and slow-flowing liquid entering the feed tank 16 by controlling the electrical signal transmission line 46 and the real-time angular velocity WIFI signal emitted by the protective cylinder angular velocity monitoring probe 66 through the cooling and slow-flowing liquid three-way valve. This ensures that the gel in the gel-specific separation and purification protective cylinder 67 remains suspended at the central axis of the protective cylinder and performs circular motion (e.g., Figure 4 (As shown). The controller 8 of the gel controllable separation and purification device controls the liquid in the membrane tube 57 to reach the membrane pressure set by the controller 8 through the electrical signal transmission line 54 of the membrane separation system reflux liquid control valve. The controller 8 of the gel controllable separation and purification device controls the start and stop of the feed pump 61 and the pressurization pump 62 through the feed pump control electrical signal transmission line 63 and the pressurization pump control electrical signal transmission line 64, respectively.

[0071] See Figure 5 The device controller panel 7 is a touch screen, including a "Feed Temperature" column, a "Liquid Level" column, a "Membrane Pressure" column, a "Angular Velocity" column, a "Run" menu, a "Stop" menu, a "OK" menu, a "Modify" menu, a "Start Feed Pump" menu, a "Stop Feed Pump" menu, a "Start Pressurization Pump" menu, a "Stop Pressurization Pump" menu, a "Start Feed Tank Temperature Control Cooling Circulation Pump" menu, a "Stop Feed Tank Temperature Control Cooling Circulation Pump" menu, a "Start Cooling Circulation Pump" menu, a "Stop Cooling Circulation Pump" menu, and a button menu (including 0, 1, 2, 3, 4, 5, 6, 7, 8, 9, ←, ↑, ↓, →, etc.).

[0072] See Figure 1The membrane separation unit is a membrane separation system suitable for spiral wound membranes. It can be modified to a membrane separation system suitable for flat sheet membranes or an electrodialysis membrane separation system according to actual needs. Both can achieve the ideal effect of efficient desalination and impurity removal of gel materials.

[0073] The device of the present invention is used as follows:

[0074] Before gel separation and purification, the gel-specific separation and purification protective tube 67 should be placed inside the feed tank 16 and fixed inside the feed tank 16 by the protective tube support rod 65 and the protective tube fixing column 70. Open the upper and lower water inlet valves 26 of the water level gauge, and then close the feed tank discharge port control valve 32 and the feed tank outlet control valve 33 at the bottom of the feed tank 16. Then manually open the feed tank liquid inlet control valve 19 to allow deionized water (if some gel materials need to be stably preserved under special cell culture medium or pH conditions, a special cell culture medium or pH buffer solution is used as the feed liquid) to slowly flow from the feed tank liquid inlet 12 into the feed tank 16 and the water level gauge 27.

[0075] Turn on the switch 2 of the temperature-controlled cooling circulation pump 3 and the switch 55 of the cooling circulation pump 48. Then turn on the device controller switch 10 of the controllable separation and purification device controller 8, click the button menu on the touch screen of the device controller panel 7, and enter the specific data of "liquid temperature" and "liquid level" in sequence. Click the "OK" menu to save the production parameters of the controllable separation and purification device controller 8. Then click the "Run" menu. At this time, the controllable separation and purification device controller 8 controls the liquid inlet control valve 19 of the liquid tank through the electrical signal transmission line 17 of the liquid tank liquid inlet control valve. When the water level gauge 27 with water level sensor detects that the liquid in the liquid tank has reached the liquid level set by the controllable separation and purification device controller 8, it automatically closes the control valve 19 of the liquid tank liquid inlet.

[0076] Clicking the "Start the temperature-controlled cooling circulation pump of the liquid tank" menu causes the condensate in the temperature-controlled cooling circulation pump 3 to enter the cooling jacket 25 of the liquid tank 16 from the temperature-controlled condensate inlet 29 via the condensate output pipeline 14, and then return to the temperature-controlled cooling circulation pump 3 from the temperature-controlled condensate return port 23 via the condensate return pipeline 13. At the same time, clicking the "Start the cooling circulation pump" menu causes the condensate in the cooling circulation pump 48 to enter the cooling flow coil 40 of the cooling flow buffer 41 from the condensate inlet 42 via the cooling flow output pipeline 56, and then return to the cooling circulation pump 48 from the condensate return port 52 via the condensate return pipeline 50.

[0077] When the temperature monitoring probe 28 of the feed tank detects that the liquid temperature in the feed tank has reached the feed temperature set by the controller 8 of the controllable separation and purification device for gel, the feed tank outlet control valve 33 is opened, the button menu on the touch screen of the device controller panel 7 is clicked, the specific data of "membrane pressure" is entered in sequence, and the "OK" menu is clicked to make the controller 8 of the controllable separation and purification device for gel save the production parameters; the "Start Feed Pump" menu and the "Start Pressurization Pump" menu are clicked in sequence to make the feed liquid enter the membrane separation system pipeline 44, feed pump 61, filter tank 59, pressurization pump 62 and membrane tube 57 with built-in spiral membrane; the "Run" menu is clicked again. At this time, the controller 8 of the controllable separation and purification device for gel controls the membrane separation system reflux control valve 60 through the electrical signal transmission line 54 of the membrane separation system reflux control valve, and automatically adjusts the liquid in the membrane tube 57 to reach the membrane pressure set by the controller 8 of the controllable separation and purification device for gel.

[0078] Through the glass viewing window 21 of the switchable cover 20, it can be observed that the liquid flows into the liquid tank 16 from the cooling and slow-flowing liquid inlet 24, causing the liquid in the liquid tank 16 and the gel-specific separation and purification protective cylinder 67 to move in a circular motion. At this time, click the button menu on the touch screen of the device controller panel 7, input the specific data of "angular velocity" in sequence, click the "OK" menu, and the gel controllable separation and purification device controller 8 saves the production parameters; then click the "Run" menu. At this time, the gel controllable separation and purification device controller 8 controls the cooling and slow-flowing liquid three-way valve 37 through the control signal transmission line 46 of the cooling and slow-flowing liquid three-way valve, and automatically adjusts the liquid in the gel-specific separation and purification protective cylinder 67 to achieve the angular velocity set by the gel controllable separation and purification device controller 8.

[0079] Open the switchable cover 20 of the feed tank, and slowly pour the gel material obtained from the gel preparation reaction into the central axis of the gel-specific separation and purification protective cylinder 67. Observe the gel's circular motion in the area near the central axis of the gel-specific separation and purification protective cylinder 67. If the gel remains suspended, close the switchable cover 20 of the feed tank. If the gel fails to suspend in the area near the central axis of the gel-specific separation and purification protective cylinder 67, click the button menu on the touch screen of the device controller panel 7, re-enter the specific data of "angular velocity", click the "OK" menu, and the controller 8 of the controllable separation and purification device for gels will save the production parameters. Then click the "Run" menu to increase the angular velocity to suspend the gel.

[0080] When the permeate flowing from the permeate outlet 58 of the membrane separation system is tested by a conductivity meter, if its conductivity is found to be extremely low (close to 0 S / m, as most gels are hydrophilic gels and are washed and stored with deionized water) or close to the conductivity of the liquid flowing into the feed tank inlet (it cannot be ruled out that some gel materials require special cell culture media or pH conditions to be stably stored, and these cell culture media or pH buffer solutions will have a certain conductivity), it can be determined that the preset endpoint has been reached, and the preparation reaction of the gel material in the gel-specific separation and purification protective cylinder has been completed. At this time, the gel is kept suspended in a circular motion near the central axis area of ​​the gel-specific separation and purification protective cylinder 67, and the gel is gently scooped out and stored using a flexible polytetrafluoroethylene screen.

[0081] It should be noted that the requirements for gel purification differ across different fields. Since most gels are hydrophilic gels, they need to be washed and stored with deionized water. Therefore, when the conductivity of the permeate flowing out of the permeate outlet of the membrane separation system is measured to be extremely low (e.g., conductivity ≤ 0.0001 S / m for gel materials used in biomedical materials or biochemical separation media; conductivity ≤ 0.001 S / m for gel materials used in the food industry; conductivity ≤ 0.01 S / m for gel materials used in daily chemical products), it can be determined that the preparation reaction of the gel material in the gel-specific separation and purification protective cartridge has been completed. If certain gel materials require specific cell culture media or pH conditions for stable preservation, these media or pH buffers will have a certain conductivity. When the conductivity of the permeate flowing from the permeate outlet of the membrane separation system is measured to be close to that of the liquid flowing into the feed tank (e.g., conductivity similarity ≥ 99.99% for gel materials used in biomedical materials or biochemical separation media; ≥ 99.00% for gel materials used in the food industry; ≥ 95.00% for gel materials used in daily chemical products), it can be determined that the preparation reaction of the gel material in the dedicated gel separation and purification protective cartridge is complete. In other words, the aforementioned preset endpoint can be a specific conductivity value or a conductivity similarity, depending on the needs of different fields.

[0082] Click the "Stop" menu on the touchscreen of the device controller panel 7, or click the "Stop Feed Pump," "Stop Pressurization Pump," "Stop Feed Tank Temperature Control Cooling Circulation Pump," and "Stop Cooling Circulation Pump" menus in sequence on the touchscreen of the device controller panel 7. The entire gel controllable separation and purification device will stop operating. Manually open the feed tank discharge port control valve 32 at the bottom of the feed tank 16 to discharge the feed from the feed tank 16 and the membrane separation unit.

[0083] It will be apparent to those skilled in the art that the present invention is not limited to the details of the exemplary embodiments described above, and that the invention can be implemented in other specific forms without departing from its spirit or essential characteristics. Therefore, the embodiments should be considered in all respects as exemplary and non-limiting, and the scope of the invention is defined by the appended claims rather than the foregoing description. Thus, all variations falling within the meaning and scope of equivalents of the claims are intended to be included within the present invention. No reference numerals in the claims should be construed as limiting the scope of the claims.

[0084] Furthermore, it should be understood that although this specification describes embodiments, not every embodiment contains only one independent technical solution. This narrative style is merely for clarity. Those skilled in the art should consider the specification as a whole, and the technical solutions in each embodiment can also be appropriately combined to form other embodiments that can be understood by those skilled in the art.

Claims

1. A controllable separation and purification device for gels, comprising a feed tank unit, a gel-specific separation and purification protective cylinder (67), a controllable separation and purification device controller (8), a cooling and slow-flow unit, and a membrane separation unit; characterized in that, The liquid tank unit includes a liquid tank (16) and a liquid tank temperature-controlled cooling circulation pump (3) that provides cooling for it. The liquid tank temperature-controlled cooling circulation pump (3) is connected to the liquid tank temperature-controlled condensate inlet (29) and the liquid tank temperature-controlled condensate return outlet (23) set on the liquid tank (16) through the liquid tank temperature-controlled cooling circulation pump condensate output pipeline (14) and the liquid tank temperature-controlled cooling circulation pump condensate return pipeline (13) to form a cooling circulation loop. The gel-specific separation and purification protective tube (67) is fixedly installed inside the liquid tank (16) by the protective tube support rod (65) and the protective tube fixing column foot (70); The membrane separation unit is connected to the outlet at the bottom of the feed tank (16) via a membrane separation system pipeline (44). The membrane separation system pipeline (44) is provided with a feed pump (61), a pressurizing pump (62), a filter barrel (59), and a membrane tube (57) in sequence along the material flow direction. The membrane tube (57) of the membrane separation unit is provided with a permeate outlet (58) of the membrane separation system, and the end pipeline is provided with a reflux control valve (60) of the membrane separation system. It also includes a conductivity meter, which is used to detect the conductivity of the permeate flowing out of the permeate outlet (58) of the membrane separation system and the liquid flowing into the feed tank inlet. The cooling and slowing flow unit is connected between the output end of the membrane separation unit and the inlet end of the feed tank (16); the cooling and slowing flow unit includes a cooling and slowing flow device (41) and a cooling and cooling circulation pump (48) that provides cooling for it. The cooling and cooling circulation pump (48) is connected to the cooling and slowing flow device (41) through a cooling and cooling circulation pump condensate output pipeline (56) and a cooling and cooling circulation pump condensate return pipeline (50) to form a cooling circulation loop; the inlet of the cooling and slowing flow device (41) is connected to the return end of the membrane separation unit through a pipeline, and its outlet is connected to the cooling and slowing flow liquid inlet (24) on the feed tank (16) through a pipeline. The controller (8) of the gel controllable separation and purification device is used to control the operation of each unit; The gel-specific separation and purification protective tube (67) includes a cylindrical protective tube wall (68), a solid protective tube bottom (69) with a closed bottom, a protective tube support rod (65) located on the upper edge of the protective tube wall (68), and a protective tube fixing column (70) located on the bottom; the protective tube wall (68) is densely covered with small holes that are obliquely cut upward from the outside of the tube to the inside; the upper edge of the protective tube wall (68) is also inlaid with a protective tube angular velocity monitoring probe (66) with a built-in WIFI signal transmitting device. The cooling and slow-flowing liquid inlet (24) is located on the side wall of the liquid tank (16). The direction in which the cooling and slow-flowing liquid inlet (24) is inserted into the liquid tank (16) is set at the tangent direction of the cross-sectional circle of the protective cylinder wall (68) at the level where the cooling and slow-flowing liquid inlet (24) is located.

2. The gel-controlled separation and purification device according to claim 1, characterized in that, The controller (8) of the gel controllable separation and purification device has a built-in WIFI signal receiver for receiving the signal emitted by the protective cylinder angular velocity monitoring probe (66); the surface of the controller (8) of the gel controllable separation and purification device is provided with a device controller panel (7), which is a touch screen for parameter setting and device control.

3. The gel-controlled separation and purification device according to claim 2, characterized in that, The liquid tank (16) is provided with an openable liquid tank cover (20) and a liquid tank inlet (12) at the top. The liquid tank inlet (12) is provided with a liquid tank inlet control valve (19). The side wall of the liquid tank (16) is provided with a water level gauge (27) and a liquid tank temperature monitoring probe (28). The bottom of the liquid tank (16) is provided with a liquid tank horizontal base (30). The liquid tank horizontal base (30) is provided with a drain hole and a discharge port below it. The discharge port is provided with a liquid tank discharge port control valve (33) and a liquid tank discharge outlet control valve (32).

4. The gel-controlled separation and purification device according to claim 3, characterized in that, The cooling and slowing flow unit also includes a cooling and slowing flow liquid three-way valve (37) and a cooling and slowing flow temperature monitoring probe (38) installed in the pipeline; the cooling and slowing flow device (41) is provided with a layered and tortuous cooling and slowing flow coil (40) inside, and the liquid to be cooled flows through the cooling and slowing flow coil (40).

5. The gel-controlled separation and purification device according to claim 4, characterized in that, The controller (8) of the gel controllable separation and purification device controls the temperature inside the feed tank (16) via the feed tank temperature control electrical signal transmission line (5) and the feed tank temperature monitoring electrical signal transmission line (18); controls the temperature of the reflux liquid via the cooling circulation pump temperature control electrical signal transmission line (11) and the cooling slow-flow liquid temperature monitoring electrical signal transmission line (45); controls the liquid level inside the feed tank (16) via the feed tank liquid inlet control valve electrical signal transmission line (17) and the water level sensor electrical signal transmission line (36); and controls the liquid level inside the feed tank (16) via the cooling circulation pump temperature control electrical signal transmission line (11) and the cooling slow-flow liquid temperature monitoring electrical signal transmission line (45). The flow rate of the liquid entering the feed tank (16) is controlled by the three-way valve of the cooling slow-flow liquid control electrical signal transmission line (46) and the angular velocity signal received is used to control the angular velocity in the gel-specific separation and purification protective tube (67); the pressure in the membrane tube (57) is controlled by the electrical signal transmission line (54) of the membrane separation system reflux liquid control valve; the start and stop of the feed pump (61) and the pressurization pump (62) are controlled by the feed pump control electrical signal transmission line (63) and the pressurization pump control electrical signal transmission line (64) respectively.

6. A method for gel separation and purification using the gel controllable separation and purification apparatus as described in claim 5, characterized in that, Includes the following steps: S1. Preparation and initialization steps: Fix the gel-specific separation and purification protective tube (67) inside the liquid tank (16), close the liquid tank discharge port control valve (32) and the liquid tank outlet control valve (33); inject liquid into the liquid tank (16) through the liquid inlet (12) until the liquid level monitored by the water level gauge (27) reaches the preset height of the gel controllable separation and purification device controller (8); S2. Temperature control steps: Start the temperature control cooling circulation pump (3) and the cooling circulation pump (48) of the liquid tank, so that the temperature of the liquid in the liquid tank (16) is reduced to and maintained at the preset low temperature by the circulating condensate of the cooling jacket (25), while the cooling buffer (41) is in standby cooling state. S3. Membrane separation system start-up steps: Open the feed tank outlet control valve (33), start the feed pump (61) and pressurization pump (62) in sequence, so that the feed liquid flows through the membrane separation system pipeline (44) and filter tank (59) into the membrane tube (57); adjust the membrane separation system reflux control valve (60) to stabilize the internal pressure of the membrane tube (57) at the preset value; S4. Circulation and Suspension Control Steps: The concentrated reflux liquid separated by the membrane tube (57) flows into the cooling and slowing flow device (41) and is rapidly cooled. Then, it enters the feed tank (16) tangentially through the cooling and slowing flow liquid inlet (24). The controller (8) of the gel controllable separation and purification device receives the real-time angular velocity signal from the protective cylinder angular velocity monitoring probe (66) and adjusts the liquid flow rate entering the feed tank (16) by regulating the cooling and slowing flow liquid three-way valve (37), so that the liquid in the gel-specific separation and purification protective cylinder (67) generates circumferential motion and reaches the preset angular velocity. Then, the gel is added to ensure that the added gel material can be suspended in the area near the central axis of the protective cylinder. S5. Endpoint Judgment and Collection Steps: Real-time detection of the conductivity of the liquid flowing out of the permeate outlet (58) of the membrane separation system. When the conductivity value reaches the preset endpoint, the gel separation and purification is determined to be complete, and the purified gel product is collected from the gel-specific separation and purification protective tube (67).

7. The method according to claim 6, characterized in that: In the circulation and suspension control step, the controller (8) of the gel controllable separation and purification device regulates the angular velocity according to the following principle: if the real-time angular velocity is lower than the preset value, the liquid flow rate into the feed tank (16) is increased; if the real-time angular velocity is higher than the preset value, the liquid flow rate into the feed tank (16) is reduced, and part of the liquid is diverted through the cooling slow flow liquid three-way valve (37) and returned to the membrane separation system pipeline (44) through the cooling slow flow liquid return pipeline (43).

8. The application of a gel-controlled separation and purification device as described in any one of claims 1-4 in the preparation of gel products, characterized in that, The applications include using the device for large-scale desalting and impurity removal of gel materials, as well as effective quality control of gel preparation reactions, wherein the gel products are selected from any of the following fields: Biomedical materials: hydrogels, medical wound dressings, or biosensor interface materials used for controlled drug release gel delivery systems or tissue engineering scaffolds; Biochemical separation media: gel packing materials for gel chromatography or agarose gel or polyacrylamide gel for separating DNA, RNA or protein; Gel core ingredients in food industry products including jelly, pudding, yogurt, gummies, or meat jelly, and daily chemical products including toothpaste, shampoo, shower gel, styling gel, or face mask.

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