Nucleic acid molecule hybridization instrument

Abstract

The embodiment of the invention provides a nucleic acid molecule hybridization instrument, and relates to the technical field of nucleic acid molecule hybridization instruments. The nucleic acid molecule hybridization instrument comprises a shell, the top of the shell is fixedly connected with a transparent cover, the top of the transparent cover is in threaded connection with a transparent cover, and an anti-skid tooth opening is formed in the periphery of an opening of the transparent cover; a sample storage assembly and a sampling assembly which are used for storing and taking samples are arranged in an inner cavity of the transparent cover, the sample storage assembly comprises a double-head motor embedded in the shell, and the sampling assembly comprises a sampling opening communicated with the front side of the transparent cover; a rotating assembly and an isolation assembly which are used for sample reaction are arranged at the bottom of an inner cavity of the shell, the rotating assembly comprises a lower electric telescopic rod fixed to an output shaft below the double-end motor through a coupler, and the isolation assembly comprises reaction bins formed in the two sides of the shell in an array mode. Multiple groups of nucleic acid samples are flexibly and effectively accessed, and an automatic isolation function is also realized.

Description

Technical Field

[0001] This invention belongs to the field of nucleic acid molecular hybridization instrument technology, and particularly relates to a nucleic acid molecular hybridization instrument. Background Technology

[0002] Nucleic acid hybridization analyzers are key scientific instruments used for nucleic acid molecular hybridization analysis. They employ flow-guided hybridization technology to improve efficiency and simplify operation. Their core technologies include a high-speed thermal circulation system, an automated mechanical lifting platform, and a pressure balancing system, enabling real-time monitoring of temperature changes.

[0003] In the prior art (the patent application with publication number CN207933435U and patent name "A Nucleic Acid Molecular Hybridization Instrument"), the protective outer shell has a good magnetic insulation effect, which effectively avoids damage to the internal sensitive components caused by the external environment, increases the safety of the equipment and extends the service life of the equipment.

[0004] During the use of nucleic acid molecular hybridization instruments, the machine cover is usually opened manually first, and the nucleic acid sample to be tested is placed in the reaction chamber and the tested nucleic acid sample is taken out. It is not possible to flexibly and effectively store and retrieve multiple sets of nucleic acid samples. Moreover, most nucleic acid molecular hybridization instruments only have a single reaction chamber, which leads to cross-infection of multiple sets of nucleic acid samples in the chamber. They do not have an automatic isolation function, which contaminates the nucleic acid samples and results in low detection accuracy. Summary of the Invention

[0005] This application aims to at least address the technical problems existing in the prior art, such as the inability to flexibly and effectively access multiple sets of nucleic acid samples and the lack of automatic isolation functions. To this end, this application proposes a nucleic acid molecular hybridization instrument.

[0006] To achieve the above objectives, the specific technical solution of the present invention is as follows: A nucleic acid molecular hybridization instrument includes a housing, a transparent cover fixedly connected to the top of the housing, and a transparent cap threadedly connected to the top of the transparent cover, wherein the transparent cap has anti-slip teeth on its circumference. The inner cavity of the transparent cover is respectively provided with a sample storage component and a sampling component for sample storage and retrieval. The sample storage component includes a dual-head motor embedded in the outer shell, and the sampling component includes a sampling port connected to the front side of the transparent cover. The bottom of the inner cavity of the outer shell is respectively provided with a rotating component and an isolation component for sample reaction. The rotating component includes a lower electric telescopic rod fixed to the output shaft below the dual-head motor by a coupling, and the isolation component includes reaction chambers arrayed on both sides of the outer shell.

[0007] Preferably, the sample storage assembly further includes an upper electric telescopic rod fixed to the output shaft above the dual-head motor via a coupling, and the piston rod of the upper electric telescopic rod is fixedly connected to an upper locking head. The top of the housing is rotatably connected to an upper locking seat that is used in conjunction with the upper locking head, and the top of the upper locking seat is fixedly connected to a placement tray that rotatably cooperates with the transparent cover. The top circumference of the placement tray is provided with a placement groove for placing samples.

[0008] Preferably, the sampling assembly further includes a channel opening on the inner side of the placement tray near the placement slot, and an electric push rod is embedded on one side of the transparent cover. The piston rod of the electric push rod is fixedly connected to a push head that interlocks with the channel opening and the sampling port, and a displacement sensor is embedded on the push head. An identification camera for use with the placement slot is embedded on the side of the transparent cover near the push head.

[0009] Preferably, the rotating assembly further includes a lower locking head fixed on the lower electric telescopic rod piston rod, and a guide rail groove is provided annularly at the bottom of the inner cavity of the outer shell. Guide rails are slidably connected around the inner cavity of the guide rail groove, and a turntable is fixedly connected to the top of the guide rails. A lower locking groove for use with the lower locking head is provided at the center of the top of the turntable.

[0010] Preferably, the isolation assembly further includes a horizontal sliding groove at the bottom of the reaction chamber, and a rotating head is fixedly connected to both sides of the top of the turntable. A support arm is hinged to the rotating head, and a connecting frame that slides with the horizontal sliding groove is hinged to the other end of the support arm. An isolation frame that is used in conjunction with the reaction chamber is fixedly connected to the top array of the connecting frame.

[0011] Preferably, a pressure sensor is embedded in the placement slot, and a warning light bead electrically connected to the pressure sensor is embedded in the circumference of the placement plate on the side near the placement slot.

[0012] Preferably, elastic heads for sample positioning are fixed in an array on both sides of the placement slot, and a marking groove with fluorescent agent is opened on the outer circumference of the placement tray near the placement slot.

[0013] Preferably, the outer shell has a circumferential slide rail groove on the side near the transparent cover, and slide rail blocks with damping sleeves are slidably connected to the inner cavity of the slide rail groove and fixed to the placement plate.

[0014] Preferably, a heater and a cooler are respectively embedded on both sides of the transparent cover, and a temperature and humidity sensor is embedded on the rear side of the transparent cover.

[0015] Preferably, the sampling port is provided with a sealing head, and a temporary groove for temporary placement of the sample is provided on the side of the outer shell near the sampling port, and a buffer pad is provided in the temporary groove.

[0016] The nucleic acid molecular hybridization instrument of the present invention has the following advantages: 1. This nucleic acid molecular hybridization instrument first controls the upper electric telescopic rod to adjust the locking stroke between the upper locking head and the upper locking seat to the correct position. Then, it controls a dual-head motor to drive the placement tray to rotate linearly through the locked upper locking head and the upper locking seat. The testing personnel can open the transparent cover and place several sets of nucleic acid samples into several placement slots on the slowly rotating placement tray. The elastic head elastically limits the nucleic acid samples placed in the placement slots and applies gravity pressure to several pressure sensors, and controls the warning light beads to light up, indicating that nucleic acid samples have been placed in the placement slot. At the same time, the heater or cooler on the transparent cover creates a suitable temperature space inside the transparent cover, and the temperature and humidity sensors monitor the temperature and humidity in the space in real time to ensure that the nucleic acid samples in the placement slots are preserved in the best environment and extend the preservation period of the nucleic acid samples. Secondly, the placement tray is controlled to slowly rotate the nucleic acid sample in the current placement slot to the sampling port. Then, the electric push rod is opened and the pusher head passes through the channel on the inside of the current placement slot, quickly pushing the nucleic acid sample forward until the required nucleic acid sample falls into the temporary slot through the sampling port. During this period, the pusher head's pushing stroke is monitored in real time by a displacement sensor to ensure that the pusher head accurately pushes the nucleic acid sample in the placement slot. The recognition camera identifies and tracks the nucleic acid sample in the rotated placement slot and the nucleic acid sample during the pushing process in real time, ensuring that the nucleic acid sample is accurately pushed into the temporary slot. It can be stored and retrieved on the spot, which is flexible and efficient.

[0017] 2. In this nucleic acid molecular hybridization instrument, the electric telescopic rod is first adjusted to the correct engagement stroke between the lower locking head and the lower locking groove. Then, with the guide rail groove and guide rail strip providing rotational support for the turntable, a dual-head motor drives the turntable to rotate linearly through the engaged lower locking head and lower locking groove. The linearly rotating turntable, via the support arms on the two rotating heads, drives two sets of connecting frames to synchronously extend or retract within the transverse sliding groove. These two sets of connecting frames drive two rows of isolation frames to synchronously extend or retract within the reaction chamber, isolating the nucleic acid samples in the isolation frames within the two rows of reaction chambers to prevent cross-contamination and improve the purity of the nucleic acid samples. Simultaneously, it facilitates the periodic disinfection and cleaning of several sets of isolation frames within the reaction chamber to prevent bacterial contamination of the nucleic acid samples.

[0018] 3. In this nucleic acid hybridization instrument, the circuit breaker is first energized to power the electromagnet, forcing the electromagnet on the synchronous pulley to electromagnetically fix the short lead screw on the fixed magnet. Then, the dual-head motor is controlled to drive the short lead screw in the positioning state through the main synchronous pulley and the driven synchronous pulley to rotate. The short lead screw drives the short lead screw drum downward. With the sliding limit cooperation of the vertical sliding groove and the limiting head, the short lead screw drum drives the extrusion seat to squeeze the pure water pre-filled in the pure water box downward. This forces the pure water in the pure water box to be quantitatively processed by the quantitative sensors on the two three-way valves, and then supplied into the nucleic acid hybridization instrument's own pipeline through the two water inlets. This allows for the periodic and quantitative replenishment of pure water to the nucleic acid hybridization instrument, reducing the frequency of manual replenishment and saving time and effort. During this process, the liquid level sensor monitors the amount of pure water in the pure water box in real time to ensure timely replenishment. Attached Figure Description

[0019] To more clearly illustrate the technical solutions of the embodiments of the present invention, the accompanying drawings used in the embodiments will be briefly introduced below. It should be understood that the following drawings only show some embodiments of the present invention and should not be regarded as a limitation of the scope. For those skilled in the art, other related drawings can be obtained from these drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of the structure of a nucleic acid molecular hybridization instrument according to the present invention; Figure 2 This is a diagram showing the open state of the isolation rack of a nucleic acid molecular hybridization instrument according to the present invention; Figure 3 This is an internal view of the structure of a nucleic acid molecular hybridization instrument according to the present invention; Figure 4 This is an exploded bottom view of the structure of the transparent cover, transparent cap, sample storage component, and sample collection component of the present invention; Figure 5 This is a partial top view of the structure of the sample storage component and the sampling component of the present invention; Figure 6 This is a top cross-sectional view of the placement disk structure of the present invention; Figure 7 This is an exploded bottom view of the transparent cover structure of the present invention; Figure 8 This is an initial state diagram of the rotating component and the isolation component structure of the present invention; Figure 9 This is a diagram showing the open state of the rotating component and the isolation component structure of the present invention; Figure 10 This is a partial exploded view of the rotating component and the isolation component structure of the present invention; Figure 11 This is a top cross-sectional view of the turntable structure of the present invention; Figure 12This is a side sectional view of the dual-head motor, transmission assembly, and water replenishment assembly of the present invention. Figure 13 This is an exploded bottom view of the dual-head motor and transmission assembly structure of the present invention; Figure 14 This is a partial exploded cross-sectional view of the transmission component and water replenishment component of the present invention. Figure 15 This is a top cross-sectional view of the outer shell structure of the present invention.

[0021] Explanation of markings in the diagram: 1. Outer shell; 2. Transparent cover; 3. Transparent cap; 41. Dual-head motor; 42. Upper electric telescopic rod; 43. Upper locking stop; 44. Upper locking stop seat; 45. Placement tray; 46. Placement slot; 51. Channel opening; 52. Electric push rod; 53. Sample pusher head; 54. Displacement sensor; 55. Recognition camera; 56. Sampling port; 61. Lower electric telescopic rod; 62. Lower locking stop; 63. Turntable; 64. Lower locking stop slot; 65. Guide rail slot; 66. Guide rail strip; 71. Reaction chamber; 72. Horizontal sliding groove; 73. Rotary head; 74. Support arm; 75. Connecting frame; 76. 81. Isolation frame; 82. Main synchronous pulley; 83. Slave synchronous pulley; 84. Circuit breaker; 85. Electromagnet; 86. Fixed magnet; 87. Short lead screw; 88. Short lead screw drum; 91. Vertical slide groove; 92. Limit head; 93. Pure water box; 94. Squeezing seat; 95. Three-way valve; 96. Quantitative sensor; 97. Water inlet; 98. Liquid level sensor; 10. Pressure sensor; 11. Warning light bead; 12. Spring head; 13. Slide rail groove; 14. Slide rail block; 15. Damping sleeve; 16. Heater; 17. Refrigerator; 18. Temperature and humidity sensor; 19. Sealing head; 20. Temporary tank. Detailed Implementation

[0022] The present invention will now be described in detail with reference to the accompanying drawings and specific embodiments: like Figures 1-15 As shown, a nucleic acid molecular hybridization instrument of the present invention includes a shell 1, a transparent cover 2 fixedly connected to the top of the shell 1, and a transparent cap 3 threadedly connected to the top of the transparent cover 2. The transparent cap 3 has anti-slip teeth around its circumference to facilitate opening and closing. A heater 16 and a cooler 17 are respectively embedded on both sides of the transparent cap 3, and a temperature and humidity sensor 18 is embedded on the rear side of the transparent cap 3. The temperature and humidity sensor 18 monitors the temperature and humidity inside the transparent cover 2 sealed by the transparent cap 3 in real time, and controls the heater 16 and the cooler 17 accordingly to heat and cool several groups of nucleic acid samples, ensuring that several groups of nucleic acid samples are in the optimal temperature environment during the storage period, and extending the storage period of nucleic acid samples. The inner cavity of the transparent cover 2 is respectively provided with a sample storage component and a sampling component for sample storage and retrieval. The sample storage component includes a dual-head motor 41 embedded in the outer shell 1, and the sampling component includes a sampling port 56 connected to the front side of the transparent cover 2. A sealing head 19 is plugged on the sampling port 56 to prevent dust from entering the sampling port 56. A temporary slot 20 is opened on the side of the outer shell 1 near the sampling port 56 for temporary placement of sample discharge. The nucleic acid sample pushed out of the sampling port 56 is temporarily stored. A buffer pad is provided in the temporary slot 20 to facilitate the buffering of nucleic acid samples that fall into the temporary slot 20. This allows for flexible and effective storage and retrieval of multiple sets of nucleic acid samples. The bottom of the inner cavity of the outer shell 1 is respectively provided with a rotating component and an isolation component for sample reaction. The rotating component includes a lower electric telescopic rod 61 fixed to the output shaft of the double-headed motor 41 via a coupling, and the isolation component includes reaction chambers 71 arrayed on both sides of the outer shell 1. It has an automatic isolation function to prevent cross-infection of several sets of nucleic acid samples and improve the detection accuracy of nucleic acid samples.

[0023] like Figures 4-11 As shown, the sample storage assembly also includes an upper electric telescopic rod 42 fixed to the output shaft above the double-head motor 41 via a coupling, and the piston rod of the upper electric telescopic rod 42 is fixedly connected to an upper locking head 43. The top of the outer shell 1 is rotatably connected to an upper locking seat 44 that is used in conjunction with the upper locking head 43. The upper electric telescopic rod 42 is controlled to adjust the engagement stroke between the upper locking head 43 and the upper locking seat 44 to the correct position. The top of the upper locking seat 44 is fixedly connected to a placement tray 45 that rotates with the transparent cover 2. The double-head motor 41 is controlled to drive the placement tray 45 to rotate linearly through the locked upper locking head 43 and the upper locking seat 44. The top circumference of the placement tray 45 is provided with a placement slot 46 for placing samples. The testing personnel can open the transparent cover 3 and place several sets of nucleic acid samples into several sets of placement slots 46 on the slowly rotating placement tray 45 in sequence to complete the differentiation and storage of several sets of nucleic acid samples. The sampling assembly also includes a channel opening 51 on the inner side of the placement tray 45 near the placement slot 46, and an electric push rod 52 is embedded on one side of the transparent cover 3. The piston rod of the electric push rod 52 is fixedly connected to a push head 53 that interlocks with the channel opening 51 and the sampling port 56. A displacement sensor 54 is embedded on the push head 53. An identification camera 55 for use with the placement slot 46 is embedded on the side of the transparent cover 3 near the push head 53. After controlling the placement tray 45 to slowly rotate the nucleic acid sample in the current placement slot 46 to the sampling port 56, the electric push rod 52 is then controlled to open and pass through the channel opening 51 on the inner side of the current placement slot 46 through the push head 53, and the nucleic acid sample is quickly pushed forward until the required nucleic acid sample falls into the temporary slot 20 through the sampling port 56. During this period, the displacement sensor 54 monitors the pushing stroke of the sample pusher 53 in real time to ensure that the sample pusher 53 accurately pushes the nucleic acid sample in the placement slot 46. The recognition camera 55 identifies and tracks the nucleic acid sample in the placement slot 46 after it has been rotated into place and the nucleic acid sample during the pushing process in real time, ensuring that the nucleic acid sample is accurately pushed into the temporary slot 20. It can be stored and retrieved on the spot, which is flexible and efficient. A pressure sensor 10 is embedded in the placement slot 46, and an alarm light bead 11 electrically connected to the pressure sensor 10 is embedded in the circumference of the placement tray 45 near the placement slot 46. The alarm light bead 11 is activated to detect the pressure of the nucleic acid sample placed in the placement slot 46, indicating that a nucleic acid sample has been placed in the slot 46. Conversely, when the nucleic acid sample is removed, the alarm light bead 11 is deactivated, indicating that the nucleic acid sample has been removed from the placement slot 46. Elastic heads for sample positioning are fixed in an array on both sides of the placement slot 46. 12. The nucleic acid samples placed in the placement slot 46 are elastically limited, and the outer circumference of the placement tray 45 near the placement slot 46 is provided with a fluorescent marking groove to mark the nucleic acid samples in each placement slot 46. The outer shell 1 is provided with a slide rail groove 13 on the side near the transparent cover 2, and the inner cavity of the slide rail groove 13 is slidably connected with a slide rail block 14 with a damping sleeve 15 and fixed to the placement tray 45, which plays a role in damping rotation support for the placement tray 45, improving the rotational stability of the placement tray 45 and preventing it from tilting and jamming.

[0024] The rotating assembly also includes a lower locking head 62 fixed on the piston rod of the lower electric telescopic rod 61, and a guide rail groove 65 is provided in the bottom of the inner cavity of the outer shell 1. Guide rails 66 are slidably connected around the inner cavity of the guide rail groove 65, and a turntable 63 is fixedly connected to the top of the guide rails 66. A lower locking groove 64 is provided at the center of the top of the turntable 63 to be used in conjunction with the lower locking head 62. After the lower electric telescopic rod 61 is adjusted to the correct engagement stroke between the lower locking head 62 and the lower locking groove 64, the turntable 63 is rotated and supported by the guide rail groove 65 and the guide rails 66. Then, the double-headed motor 41 is controlled to drive the turntable 63 to rotate linearly through the engaged lower locking head 62 and the lower locking groove 64. The isolation assembly also includes a horizontal sliding groove 72 at the bottom of the reaction chamber 71, and two rotating heads 73 are fixedly connected to the top of the turntable 63 on both sides. A support arm 74 is hinged to the rotating head 73, and a connecting frame 75 that slides in cooperation with the horizontal sliding groove 72 is hinged to the other end of the support arm 74. The linearly rotating turntable 63 drives the two sets of connecting frames 75 to move outward or inward synchronously within the horizontal sliding groove 72 through the support arms 74 on the two rotating heads 73. The top array of the connecting frames 75 is fixedly connected to the isolation frames 76 used in conjunction with the reaction chamber 71. The two sets of connecting frames 75 drive the two rows of isolation frames 76 to move outward or inward synchronously within the reaction chamber 71, which plays a role in isolating the nucleic acid samples in the isolation frames 76 in the two rows of reaction chambers 71 to prevent cross-contamination of nucleic acid samples, improve the purity of nucleic acid samples, and facilitate the regular disinfection and cleaning of several sets of isolation frames 76 in the reaction chamber 71 to prevent bacteria from growing and contaminating the nucleic acid samples.

[0025] like Figures 12-14 As shown, during the use of the nucleic acid molecular hybridization instrument, it is necessary to replenish pure water periodically. This operation is basically done manually, as it does not have an automatic quantitative pure water replenishment function, which is quite cumbersome. The rear side of the inner cavity of the outer shell 1 is equipped with a transmission component and a water replenishment component. The transmission component includes a main synchronous pulley 81 fixed on the upper electric telescopic rod 42. The outer side of the main synchronous pulley 81 is connected to a driven synchronous pulley 82 via a synchronous belt. A circuit breaker 83 is fixedly connected to the rear side of the outer shell 1. An electromagnetic device electrically connected to the circuit breaker 83 is embedded in the driven synchronous pulley 82. The electromagnet 84 is equipped with a fixed magnet 85 at its bottom. The circuit breaker 83 energizes the electromagnet 84, forcing the electromagnet 84 on the synchronous pulley 82 to electromagnetically fix the short lead screw 86 on the fixed magnet 85. The bottom of the fixed magnet 85 is fixedly connected to the short lead screw 86, and the short lead screw 86 is threadedly connected to the short lead screw 87. The dual-head motor 41 drives the short lead screw 86 in the positioning state to rotate through the main synchronous pulley 81 and the slave synchronous pulley 82. The short lead screw 86 drives the short lead screw 87 to move downward. The water replenishment component includes a vertical sliding groove 91 located on the outer casing 1 near the short wire tube 87. A limiting head 92, fixed to the edge of the short wire tube 87, is slidably connected within the vertical sliding groove 91. A squeezing seat 94 is located at the bottom of the short wire tube 87, and a pure water box 93, fitted into the outer casing 1, is slidably connected to the outside of the squeezing seat 94. A water inlet with a plug is connected to the back of the pure water box 93. With the vertical sliding groove 91 and the limiting head 92 providing sliding limiting for the short wire tube 87, the short wire tube 87 drives the squeezing seat 94 to squeeze the pre-filled pure water in the pure water box 93 downwards. Both sides of the pure water box 93 are connected to a water inlet with a plug. The quantitative sensor 96 has a three-way valve 95, and the outer end of the three-way valve 95 is connected to a water inlet 97 that is connected to the pipeline of the nucleic acid molecular hybridization instrument. A liquid level sensor 98 is embedded on one side of the bottom of the pure water box 93. This forces the pure water in the pure water box 93 to be quantitatively processed by the quantitative sensors 96 on the two three-way valves 95, and then supplied into the pipeline of the nucleic acid molecular hybridization instrument through the two water inlets 97. This provides the nucleic acid molecular hybridization instrument with pure water regularly and quantitatively, reducing the frequency of manual filling and saving time and effort. During this process, the liquid level sensor 98 monitors the amount of pure water in the pure water box 93 in real time so as to replenish the pure water in time.

[0026] The working principle of a nucleic acid molecular hybridization instrument is as follows: When storing several sets of nucleic acid samples, after opening the transparent cover 3 on the transparent cover 2, first control the upper electric telescopic rod 42 to open and drive the upper locking head 43 to move upward and lock into the upper locking seat 44. With the damping sleeve 15 on the slide rail groove 13 and slide rail block 14 damping the rotation of the placement plate 45, then control the double-head motor 41 to open and drive the placement plate 45 on the upper locking seat 44 to rotate linearly through the locked upper locking head 43. The linear placement plate 45 drives the placement slot 46 to rotate slowly, and several sets of nucleic acid samples are placed into the slowly rotating placement slots 46 in sequence. The spring head 12 is then used to place the samples into the placement slots 46. Nucleic acid samples in slot 6 are elastically limited, and pressure sensors 10 in each slot 46 are pressed down by gravity. At this time, the corresponding warning light 11 lights up, indicating that a nucleic acid sample has been placed in the slot 46. Alternatively, several sets of nucleic acid samples can be placed directly in several sets of slots 46 in the static state of the placement tray 45. During this period, the temperature and humidity sensor 18 on the transparent cover 3 in the sealed state monitors the temperature and humidity of the environment in the several sets of slots 46 in real time. The heater 16 or the cooler 17 can be controlled to heat or cool the environment in which the several sets of nucleic acid samples are located, so that the several sets of nucleic acid samples are in the best preservation environment. When multiple sets of nucleic acid samples need to be sampled, the placement tray 45 is first controlled to drive multiple placement slots 46 to rotate linearly and slowly. When the nucleic acid sample in the placement slot 46 to be sampled rotates to the recognition camera 55, the recognition camera 55 quickly takes a picture of the nucleic acid sample to be sampled and identifies it. Then, the dual-head motor 41 is controlled to stop the placement tray 45. Immediately afterward, the electric push rod 52 is controlled to open and drive the sample pusher 53 to pass through the channel opening 51 in the placement slot 46 and push it forward. The nucleic acid sample in the placement slot 46 is then pushed forward and passes through... The sampling port 56 of the sealing head 19 is removed in advance and falls onto the buffer pad in the temporary slot 20. During this period, the displacement sensor 54 monitors the pushing stroke of the sample pusher 53 in real time, and the identification camera 55 tracks the nucleic acid sample image during the pushing process in real time to ensure that the nucleic acid sample in the current placement slot 46 is sampled. First, the dual-head motor 41 is paused, and the electric push rod 52 is closed to drive the sample pusher 53 to return to the reset position. Then, the upper electric telescopic rod 42 is closed and the upper locking stop head 43 is moved down to disengage from the upper locking stop seat 44 to the initial position. Next, the electric telescopic rod 61 is first opened, causing the lower locking head 62 to move down and engage with the lower locking groove 64 of the turntable 63. With the guide rail groove 65 and guide rail 66 providing rotational limit for the turntable 63, the dual-head motor 41 is then reopened, and the engaged lower locking head 62 drives the turntable 63 to rotate linearly. The turntable 63, through two rotating heads 73, drives two support arms 74 to extend linearly outwards synchronously. The two support arms 74 drive two sets of connecting frames 75 to slide synchronously outwards within the transverse sliding groove 72, thus... The two rows of isolation racks 76 detach from the reaction chamber 71 to the outside of the outer shell 1 until the two rows of isolation racks 76 extend to the outermost side. Then, the nucleic acid samples to be tested are placed into the two rows of isolation racks 76 in sequence. Conversely, after controlling the two rows of isolation racks 76 to return to the reaction chamber 71, the dual-head motor 41 is first controlled to pause, and then the lower electric telescopic rod 61 is controlled to close and drive the lower locking head 62 to move up and disengage from the lower locking groove 64 of the turntable 63 to the initial position. Thus, each nucleic acid sample to be tested is isolated to form an independent reaction and testing space. Then, the circuit breaker 83 is first energized to the electromagnet 84. The electromagnet 84 on the synchronous pulley 82, via the fixed magnet 85, electromagnetically fixes the short lead screw 86. Then, the double-headed motor 41 is restarted, and the synchronous belt on the main synchronous pulley 81 drives the driven synchronous pulley 82 to rotate. The driven synchronous pulley 82, via the electromagnet 84 and the fixed magnet 85, drives the positioned short lead screw 86 to rotate. With the vertical sliding groove 91 and the limiting head 92 providing sliding limit cooperation for the short lead drum 87, the short lead screw 86 drives the short lead drum 87 to move downwards. The filament tube 87 drives the extrusion seat 94 to slide down inside the pure water box 93, applying downward pressure to the pure water that has been added to the pure water box 93 in advance. This forces the pure water in the pure water box 93 to be quantitatively monitored and processed by the quantitative sensors 96 on the two three-way valves 95. Then, the quantitative pure water is supplied into the pipeline of the nucleic acid molecular hybridization instrument through the two water inlets 97. During this process, the pure water level in the pure water box 93 is monitored in real time by the liquid level sensor 98 so that pure water can be added to the pure water box 93 in a timely manner. Thus, the quantitative filling of pure water into the nucleic acid molecular hybridization instrument is completed.

[0027] It should be noted that the specific models and specifications of the dual-head motor 41, upper and lower electric telescopic rods, electric push rod 52, identification camera 55, circuit breaker 83, electromagnet 84, heater 16, cooler 17, and various valves and sensors need to be selected and determined according to the actual specifications of the device. The specific selection and calculation method adopts the existing technology in this field, so it will not be elaborated in detail.

[0028] The power supply circuits for the dual-head motor 41, the upper and lower electric telescopic rods, the electric push rod 52, the identification camera 55, the circuit breaker 83, the electromagnet 84, the heater 16, the cooler 17, and various valves and sensors are clear to those skilled in the art and will not be described in detail here.

[0029] It is understood that the present invention has been described through some embodiments, and those skilled in the art will recognize that various changes or equivalent substitutions can be made to these features and embodiments without departing from the spirit and scope of the invention. Furthermore, under the teachings of the present invention, these features and embodiments can be modified to adapt to specific situations and materials without departing from the spirit and scope of the invention. Therefore, the present invention is not limited to the specific embodiments disclosed herein, and all embodiments falling within the scope of the claims of this application are within the protection scope of the present invention.

Claims

1. A nucleic acid molecular hybridization instrument, comprising a shell (1), characterized in that: The top of the outer shell (1) is fixedly connected to a transparent cover (2), and the top of the transparent cover (2) is threadedly connected to a transparent cap (3). The transparent cap (3) has anti-slip teeth on its circumference. The inner cavity of the transparent cover (2) is respectively provided with a sample storage component and a sampling component for sample storage and retrieval. The sample storage component includes a double-headed motor (41) embedded in the outer shell (1), and the sampling component includes a sampling port (56) connected to the front side of the transparent cover (2). The bottom of the inner cavity of the outer shell (1) is respectively provided with a rotating component and an isolation component for sample reaction. The rotating component includes a lower electric telescopic rod (61) fixed to the output shaft of the double-headed motor (41) by a coupling, and the isolation component includes reaction chambers (71) arrayed on both sides of the outer shell (1).

2. The nucleic acid molecular hybridization instrument according to claim 1, characterized in that: The sample storage assembly also includes an upper electric telescopic rod (42) fixed to the output shaft above the double-head motor (41) via a coupling, and the piston rod of the upper electric telescopic rod (42) is fixedly connected to an upper locking head (43). The top of the outer shell (1) is rotatably connected to an upper locking seat (44) that is used in conjunction with the upper locking head (43), and the top of the upper locking seat (44) is fixedly connected to a placement tray (45) that rotatably cooperates with the transparent cover (2). The top circumference of the placement tray (45) is provided with a placement groove (46) for placing samples.

3. The nucleic acid molecular hybridization instrument according to claim 2, characterized in that: The sampling assembly also includes a channel opening (51) on the inner side of the placement tray (45) near the placement slot (46), and an electric push rod (52) is embedded on one side of the transparent cover (3). The piston rod of the electric push rod (52) is fixedly connected to a push head (53) that interlocks with the channel opening (51) and the sampling port (56). A displacement sensor (54) is embedded on the push head (53). An identification camera (55) for use with the placement slot (46) is embedded on the side of the transparent cover (3) near the push head (53).

4. The nucleic acid molecular hybridization instrument according to claim 3, characterized in that: The rotating assembly also includes a lower locking head (62) fixed on the piston rod of the lower electric telescopic rod (61), and a guide rail groove (65) is provided in the bottom of the inner cavity of the outer shell (1). Guide rails (66) are slidably connected around the inner cavity of the guide rail groove (65), and a turntable (63) is fixedly connected to the top of the guide rails (66). A lower locking groove (64) is provided at the center of the top of the turntable (63) to be used in conjunction with the lower locking head (62).

5. A nucleic acid molecular hybridization instrument according to claim 4, characterized in that: The isolation assembly also includes a horizontal sliding groove (72) at the bottom of the reaction chamber (71), and a rotating head (73) is fixedly connected to both sides of the top of the turntable (63). A support arm (74) is hinged to the rotating head (73), and a connecting frame (75) that slides with the horizontal sliding groove (72) is hinged to the other end of the support arm (74). An isolation frame (76) that is used in conjunction with the reaction chamber (71) is fixedly connected to the top array of the connecting frame (75).

6. A nucleic acid molecular hybridization instrument according to claim 5, characterized in that: The placement slot (46) is equipped with a pressure sensor (10), and the placement plate (45) is equipped with a warning light bead (11) that is electrically connected to the pressure sensor (10) on one side of the circumference near the placement slot (46).

7. A nucleic acid molecular hybridization instrument according to claim 6, characterized in that: Both sides of the placement slot (46) are fixed with elastic heads (12) for sample positioning, and the placement plate (45) has a fluorescent marker groove on the outer circumference near the placement slot (46).

8. A nucleic acid molecular hybridization instrument according to claim 7, characterized in that: The outer shell (1) has a circumferential slide rail groove (13) on the side near the transparent cover (2), and slide rail blocks (14) with damping sleeves (15) are slidably connected around the inner cavity of the slide rail groove (13) and fixed to the placement plate (45).

9. A nucleic acid molecular hybridization instrument according to claim 8, characterized in that: A heater (16) and a cooler (17) are respectively embedded on both sides of the transparent cover (3), and a temperature and humidity sensor (18) is embedded on the rear side of the transparent cover (3).

10. A nucleic acid molecular hybridization instrument according to claim 9, characterized in that: The sampling port (56) is plugged with a sealing head (19), and the outer shell (1) is provided with a temporary groove (20) for temporary placement of sample discharge on the side near the sampling port (56), and a buffer pad is provided inside the temporary groove (20).

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