Probiotic fermentation amplification equipment and method

Abstract

The invention relates to but not limited to the technical field of probiotic fermentation equipment, and discloses probiotic fermentation amplification equipment and method.The probiotic fermentation amplification equipment comprises a base, a barrel body arranged on the top of the base, a feeding pipe arranged on the outer side of the barrel body, a hollow top cover arranged on the top of the barrel body and a wave making pump arranged on the inner side of the base; the sampling unit is used for collecting a probiotic fermentation liquor sample in the barrel body. According to the probiotic fermentation amplification equipment, through cooperation of the sampling pipeline and the sampling holes, when sampling is needed, fermentation liquor in the sampling pipeline is sucked into the sampling holes, the driving assembly drives the rotating ring to rotate to switch the sampling holes in butt joint with the sampling pipeline, and the fermentation liquor in the sampling pipeline is sucked into the other sampling hole; the different sampling holes are used for containing different layers of fermentation liquor in the sampling pipeline, so that fermentation liquor samples in each area can be obtained, and an enforcer can judge the fermentation liquor in the barrel body more accurately.

Description

Technical Field

[0001] This invention relates to the technical field of probiotic fermentation equipment, but is not limited to it. In particular, it relates to a probiotic fermentation amplification device and method. Background Technology

[0002] Probiotics are a type of live microorganism that are beneficial to humans. They are mainly found in the human gut and reproductive system. They can help maintain gut health, enhance the function of the immune system, improve intestinal digestion and defecation, and prevent infections.

[0003] Probiotic fermentation and amplification equipment is used to increase the number of probiotics and is widely used in probiotic production, food, and pharmaceutical fields. This type of equipment typically employs sampling fermentation technology, controlling fermentation conditions such as temperature, pH, and nutrient concentration to increase the number of probiotics. Continuous sampling and testing are required during the fermentation process to further adjust the fermentation conditions.

[0004] Patent publication number CN213739439U discloses a probiotic fermentation tank, relating to the field of fermentation tank technology, specifically a probiotic fermentation tank. The tank includes a base, with an insulation layer abutting the top of the base. The tank body abuts the inside of the insulation layer, and a fixing plate is fixedly installed on the top of the tank body via a support rod. This probiotic fermentation tank, through the design of a connecting pipe and an observation chamber, achieves the effect of observing the internal fermentation process without the need for external connecting pipes. The combination of a sampling tube and an observation window allows for observation of the internal fermentation process through the glass observation window and connecting pipe. The sampling tube facilitates the extraction of small amounts of fermented material from the tank for sampling and testing, resulting in a simple structure, convenient operation, and high practicality.

[0005] The aforementioned patent has the following defects:

[0006] During the fermentation process, due to factors such as the sedimentation and stratification of nutrients and uneven temperature distribution in the fermentation tube, the fermentation state of probiotics in different areas of the fermentation tank is different. Therefore, collecting sample information from only one area in the fermentation tube cannot accurately reflect the true situation in the fermentation tube and may lead the implementer to make incorrect judgments.

[0007] Mixing equipment is often installed inside fermentation tubes, but the range of influence of the mixing equipment is limited, and it is impossible to make targeted adjustments based on the conditions inside the fermentation tubes. Summary of the Invention

[0008] In view of the problem that when the inlet of a spiral centrifugal pump is blocked, the spiral impeller continues to rotate, and the lubrication and cooling inside the pump cannot be carried out normally, causing the impeller to run dry. This may lead to overheating and damage inside the pump, affecting the normal use of the pump. Therefore, a probiotic fermentation and amplification device and method are proposed.

[0009] One aspect of this application provides a probiotic fermentation and amplification device, the purpose of which is to prevent the spiral impeller from running dry when the inlet is blocked and water cannot enter the pump body, thereby avoiding overheating and damage inside the pump.

[0010] The technical solution of the present invention is as follows: a probiotic fermentation and amplification device, comprising a base, a barrel body disposed on the top of the base, a feeding pipe disposed on the outside of the barrel body, a hollow top cover disposed on the top of the barrel body, a wave-making pump disposed on the inside of the base, a cover plate disposed on the top of the hollow top cover, a suction device disposed on the top of the cover plate, a heating rod and an ultraviolet lamp disposed on the bottom of the hollow top cover, and further comprising a sampling unit for collecting probiotic fermentation liquid samples inside the barrel body;

[0011] The sampling unit includes a mounting ring disposed between the barrel body and the hollow top cover, a mounting port disposed at the top of the mounting ring, a sampling pipe disposed inside the mounting port, a hollow boss disposed at the bottom inner side of the hollow top cover, a rotating ring disposed inside the hollow top cover, a sampling hole disposed on the surface of the rotating ring, a suction channel and a drainage channel disposed between the inner ring wall of the rotating ring and the sampling hole, a docking hole disposed on the outer side of the hollow boss, a suction pipe disposed on the outer side of the suction device, and an insertion hole disposed at the bottom of the rotating gear.

[0012] The rotating ring is rotatably connected to the outside of the hollow boss. The sampling holes are arranged in a ring array with the central axis of the rotating ring as the reference. The number of sampling holes is an integer multiple of the number of sampling pipes. The drain channel extends into the hollow boss. A one-way flap is set inside the drain channel. The docking hole is set to correspond to the sampling pipe. The docking hole and the suction channel are docked. The bottom end of the suction tube penetrates downward into the hollow boss. A sealing gasket is set at the bottom of the rotating ring. The top end of the sampling pipe extends into the insertion hole.

[0013] The sampling unit further includes a driving component and a blocking component. The driving component is used to provide driving force to the rotating ring and to selectively block the suction channel.

[0014] The above sampling scheme, through the cooperation of sampling pipes and sampling holes, requires the suction device to generate suction force when sampling is needed. The suction force is transmitted to the sampling hole, and the fermentation liquid in the sampling pipe is sucked into the sampling hole. The drive component drives the rotating ring to rotate and switch the sampling hole connected to the sampling pipe. The fermentation liquid in the sampling pipe is sucked into another sampling hole. Different sampling holes hold different layers of fermentation liquid in the sampling pipe. In this way, fermentation liquid samples from various areas can be obtained. The operator can make a more accurate judgment on the fermentation liquid in the tank, and can also quickly identify abnormal fermentation areas. The fermentation situation in abnormal areas can be improved by adjusting the wave pump and heating rod.

[0015] Furthermore, the drive assembly includes a power groove disposed at the top of the rotating ring, a power gear disposed at the bottom of the cover plate, and a drive motor disposed at the top of the cover plate;

[0016] The inner wall of the power groove is provided with teeth, the power gear is located inside the power groove, the outer teeth of the power gear are only half a circle, the power gear meshes with the teeth on the inner wall of the power groove, and the power gear is connected to the power output shaft of the drive motor.

[0017] The above sampling scheme was adopted. By using a power gear with only half a turn of the tooth, the rotating ring rotates when the teeth of the power gear mesh with the power groove, and stops when the teeth separate, allowing the sampling hole sufficient time to draw up the fermentation liquid.

[0018] Furthermore, the drive assembly also includes a drive groove disposed at the bottom of the inner side of the hollow top cover, and a drive ring disposed at the bottom of the rotating ring;

[0019] The sampling conduit includes a fixed tube and a rotating tube, both of which are semi-circular pipes. Both the fixed tube and the rotating tube are inserted into the installation port. The rotating tube is rotatably connected to the inner wall of the fixed tube. A rotating gear is provided on the outer side of the rotating tube. The drive groove is connected to the insertion hole. A drive ring is rotatably connected inside the drive ring. The inner ring surface of the drive ring is provided with teeth, and the length of the teeth is half the circumference of the rotating gear. The number of teeth on the inner ring surface of the drive ring is the same as the number of sampling pipes. The rotating gear meshes with the teeth on the inner ring surface of the drive ring.

[0020] The above sampling scheme involves setting up a sampling pipeline. When the rotating ring rotates, the driving ring drives the rotating tube to rotate, combining the fixed tube and the rotating tube into a complete pipeline. During aspiration, the fermentation broth in the pipeline gradually enters the sampling holes from top to bottom. After sampling is completed, the rotating ring rotates, driving the rotating tube to rotate into the fixed tube, thus fully opening the sampling pipeline. The fermentation broth in the sampling pipeline interacts with the fermentation broth in various areas of the barrel, allowing for more comprehensive sample acquisition. Simultaneously, the open sampling pipeline restricts pressure, causing fermentation broth above the surface of the fermentation broth in the barrel to flow back into the barrel. As the rotating ring rotates, the fermentation broth in each sampling hole is emptied, ensuring the accuracy of continuous sampling.

[0021] Furthermore, the blocking assembly includes a slot disposed on the top of the rotating ring, a sleeve disposed inside the slot, a drain channel disposed between the slot and the sampling hole, a mating interface disposed on the outside of the sleeve, a blocking ball disposed inside the sleeve, and a top rod disposed on the top of the sleeve.

[0022] The slot and the suction channel intersect, the sleeve cuts off the suction channel, the discharge channel extends into the inside of the sleeve, the interface is connected to the suction channel, and the material density of the sealing ball is less than the density of the fermentation liquid.

[0023] By setting up the above sampling scheme and installing a blocking component, as the suction continues, the amount of fermentation liquid in the sampling hole increases. After the fermentation liquid enters the suction channel, it flows into the sleeve, and the sealing ball floats up, blocking the interface and cutting off the suction channel. This prevents the fermentation liquid from entering the hollow boss and ensures the stability of continuous sampling.

[0024] Furthermore, the sampling unit also includes a sampling component for extracting fermentation broth samples;

[0025] The sampling assembly includes a sampling port disposed on the top of the cover plate, a connecting tube disposed inside the sampling port, a return spring disposed between the outer wall of the connecting tube and the inner wall of the sampling port, a plug disposed on the top of the connecting tube, a fixing bracket disposed on the top of the cover plate, a tube rack disposed on the top of the fixing bracket, and a sampling tube disposed on the top of the tube rack.

[0026] The sampling port penetrates the cover plate and its distribution corresponds to the sampling hole. The sampling tube penetrates the pipe rack and its distribution corresponds to the sampling port. The connection between the fixed frame and the pipe rack has a sampling telescopic structure.

[0027] The above sampling method involves setting up a sampling component. After all sampling holes are filled with fermentation broth samples, the tube holder is pressed, and the sampling tube moves downward to contact the plug. The plug is inserted into the push rod, and the connecting tube moves downward into the sampling hole. The sampling tube then draws out the fermentation broth from the sampling hole, completing the sampling process.

[0028] Furthermore, the sampling assembly also includes a sliding groove disposed on the top of the cover plate, a baffle plate disposed inside the sliding groove, a buckle disposed on the baffle plate and the surface of the tube rack, and a hook disposed between the two buckles.

[0029] The sliding groove covers the upper opening of the sampling port, and the diameter of the push rod is smaller than the diameter of the sleeve of the interface.

[0030] When the tube rack is pressed down, the shield is pushed open by the hook, exposing the connecting tube and plug inside the sampling port. When the tube rack is reset upward, the hook pulls the shield back to close the sampling port, reducing the entry of dust and bacteria into the sampling port and preventing contamination of the fermentation broth sample.

[0031] Furthermore, the fixed tube and cover plate are also equipped with a finished product collection unit for acquiring the fermentation broth after amplification;

[0032] The finished product collection unit includes a control component located on the top of the cover plate, used to control the opening and closing of the suction channel;

[0033] The control component includes a push rod disposed on the top of the cover plate, a push plate disposed on the top of the push rod, a push threaded rod disposed on the top of the push plate, and a pressure plate disposed at the bottom of the push rod.

[0034] The push rod is slidably inserted above the cover plate, and the lower end of the push rod extends to the bottom of the cover plate. The pressure plate is located above the top rod, the push thread rod passes through the push plate, the push plates are rotatably connected to each other, and the push plate is threadedly connected to the cover plate.

[0035] The above sampling scheme, through the setting of control components, allows for the rotation of the push threaded rod when a fermentation broth sample is needed. The push plate, via the push rod, presses down on the pressure plate, which in turn presses the push rod into the sleeve, preventing the sealing ball from floating upwards. This ensures that the suction channel remains unobstructed, allowing fermentation broth to be collected from multiple sampling pipes and then gathered within the hollow boss, resulting in a more consistent quality of the fermentation broth extracted each time.

[0036] Furthermore, the finished product collection unit also includes a suction component disposed on the outside of the fixed tube for collecting fermentation broth at different depths;

[0037] The suction assembly includes a suction port disposed on the outside of the fixed tube, a movable sliding groove disposed on the surface of the fixed tube, a baffle disposed on the surface of the suction port, a slot disposed on the side of the baffle near the suction port, a rotating shaft disposed inside the suction port, a pull rod disposed on the outside of the rotating shaft, and a lever disposed on the end of the pull rod away from the baffle.

[0038] The movable chute is distributed on both sides of the suction port. The cover plate is slidably connected to the movable chute. The material density of the cover plate is less than that of the fermentation liquid. A torsion spring is set between the pull rod and the rotating shaft, and a torsion spring is set between the pull rod and the lever plate. The force required for the torsion spring to stretch is less than that required for the torsion spring to stretch. The lever plate can only deflect in one direction.

[0039] The above sampling scheme, through the setting of a separate suction component, involves rotating the tube clockwise during sampling. The rotating tube contacts the deflector plate, causing the deflector plate to deflect. The shield plate remains unaffected, always keeping the suction port closed. When using the fermentation broth sample, rotating the tube counterclockwise causes the rotating tube to contact the deflector plate. The deflector plate drives the pull rod to deflect, causing the pull rod to move out of the slot. At this time, the shield plate located in the fermentation broth floats up, opening the suction port. This allows the sampling pipe to suction fermentation broth from different areas within the container, resulting in a more uniform quality of the suctioned fermentation broth. As the fermentation broth continues to decrease during suction, the shield plate above the fermentation broth loses buoyancy and slides down to seal the suction port, preventing air from entering the sampling pipe during suction. This makes the suction process more stable and efficient.

[0040] Furthermore, the present invention also provides a method for probiotic fermentation amplification, comprising the following steps:

[0041] Step 1: Preparation: First, clean the entire set of equipment with tap water to keep it clean. Move the equipment to a room equipped with UV lamps. Then, install the UV lamps, heating rods, and wave generators onto the base and hollow top cover. Adjust the heating rods to a constant temperature of 35℃.

[0042] Step 2: Prepare the solution: Add brown sugar and water to the container in the correct proportions;

[0043] Step 3; Sterilization: Turn on the wavemaker pump, UV lamp and heater, UV lamp disinfection for 4 hours, water temperature maintained at 35℃;

[0044] Step 4: Inoculation and Cultivation: After sterilization, turn off the UV lamp inside the container, add probiotics according to the ratio, cover with the hollow top lid, and ferment at a constant temperature for 48 hours. Maintain the temperature at approximately 35℃.

[0045] Step 5: Regularly check the equipment operation, maintain a constant temperature, ensure the wavemaker pump is running normally, and incubate for 48 hours;

[0046] Step 6: After successful amplification, turn off the wavemaker pump and heating rod, and take out the fermentation liquid in batches according to the product usage recommendations. The fermentation liquid must be used up within 7 days. When taking out the liquid multiple times, keep the hollow top cap sealed.

[0047] Step 7: After the fermentation liquid is used up, the base, barrel, heating rod, wave pump, ultraviolet lamp, etc. should be thoroughly rinsed with tap water. There should be no foreign matter or impurities left, and no odor, so that it can be used next time.

[0048] By sampling the above scheme and using the new fermentation process, the quality of probiotics is better and the amplification efficiency is higher.

[0049] Furthermore, in step four, the wave-generating pump is kept running continuously during the fermentation process, and the ultraviolet lamps in the room are kept on all the time, but are turned off when personnel are working.

[0050] The beneficial effects of this invention are:

[0051] 1. By coordinating the sampling pipes and sampling holes, when sampling is required, the suction device generates suction force, which is transmitted to the sampling hole. The fermentation liquid in the sampling pipe is sucked into the sampling hole. The drive component drives the rotating ring to rotate and switch the sampling hole connected to the sampling pipe. The fermentation liquid in the sampling pipe is sucked into another sampling hole. Different sampling holes hold different layers of fermentation liquid in the sampling pipe. In this way, fermentation liquid samples from various areas can be obtained. The operator can make a more accurate judgment on the fermentation liquid in the tank. At the same time, abnormal fermentation areas can be quickly identified, and the fermentation situation in abnormal areas can be improved by adjusting the wave pump and heating rod.

[0052] 2. By setting up a sampling pipeline, when the rotating ring rotates, the driving ring drives the rotating tube to rotate, so that the fixed tube and the rotating tube are combined into a complete pipeline. During aspiration, the fermentation liquid in the pipeline enters the sampling hole little by little from top to bottom. After sampling is completed, the rotating ring rotates, and the driving ring drives the rotating tube to rotate. The rotating tube rotates into the fixed tube, so that the sampling pipeline is fully open. The fermentation liquid in the sampling pipeline interacts with the fermentation liquid in various areas of the barrel, so that the sample obtained by the sampling pipeline is more comprehensive. At the same time, after the sampling pipeline is open, its pressure is limited, and the fermentation liquid above the fermentation liquid level in the barrel flows back into the barrel. As the rotating ring rotates, the fermentation liquid in each sampling hole is emptied, ensuring the accuracy of continuous sampling.

[0053] 3. By setting up a blocking component, as the suction continues, the amount of fermentation liquid in the sampling hole increases. After the fermentation liquid enters the suction channel, it flows into the sleeve, and the sealing ball floats up, blocking the interface and cutting off the suction channel. This prevents the fermentation liquid from entering the hollow boss and ensures the stability of continuous sampling.

[0054] 4. By setting up a sampling assembly, when sampling, the rotating tube rotates clockwise, contacting the deflector plate. The deflector plate deflects, while the shield plate remains unaffected, always keeping the suction port closed. When using fermentation broth samples, the rotating tube rotates counterclockwise, contacting the deflector plate. The deflector plate drives the pull rod to deflect, and the pull rod moves out of the slot. At this time, the shield plate located in the fermentation broth floats up, and the suction port opens, allowing the sampling pipe to draw fermentation broth from different areas within the container. This results in a more uniform quality of the drawn fermentation broth. As the fermentation broth continues to decrease during sampling, the shield plate above the fermentation broth loses buoyancy and slides down to seal the suction port, preventing air from entering the sampling pipe during sampling. This makes the sampling process more stable and efficient. Attached Figure Description

[0055] Figure 1 This is a perspective view of Embodiment 1 of the present invention;

[0056] Figure 2 This is an anatomical diagram of the barrel body and hollow top cover of the present invention;

[0057] Figure 3 This is an anatomical diagram of the mounting ring and hollow top cover of the present invention;

[0058] Figure 4 This is a schematic diagram of the bottom of the hollow top cover of the present invention;

[0059] Figure 5 This is an anatomical diagram of the sampling pipe and mounting ring of the present invention;

[0060] Figure 6 This is a schematic diagram of the sampling pipe suction state of the present invention;

[0061] Figure 7 This is a schematic diagram of the sampling pipe in the open state of the present invention;

[0062] Figure 8 This is a schematic diagram of the top of the hollow top cover of the present invention;

[0063] Figure 9 This is an anatomical diagram of the hollow top cover and cover plate of the present invention;

[0064] Figure 10 This is an anatomical diagram of the hollow top cover and rotating ring of the present invention;

[0065] Figure 11 This is a partial sectional view of the hollow top cover of the present invention;

[0066] Figure 12 This is a schematic diagram of the interior of the hollow top cover of the present invention;

[0067] Figure 13 This is a schematic diagram of the bottom of the rotating ring of the present invention;

[0068] Figure 14 This is a schematic diagram of the hollow boss of the present invention;

[0069] Figure 15 This is a schematic diagram of the rotating ring of the present invention;

[0070] Figure 16 This is a schematic diagram of the inside of the sleeve of the present invention;

[0071] Figure 17 This is a schematic diagram of the top of the cover plate of the present invention;

[0072] Figure 18 This is a diagram showing the connection between the sampling component and the control component of the present invention.

[0073] Figure 19 This is a schematic diagram of the bottom of the cover plate of the present invention;

[0074] Figure 20 This is a schematic diagram of the inside of the sampling port of the present invention;

[0075] Figure 21 This is a schematic diagram of the sampling component of the present invention;

[0076] Figure 22 This is a schematic diagram of the outer surface of the fixing tube of the present invention;

[0077] Figure 23 This is a schematic diagram of the interior of the fixed tube and the rotating tube of the present invention;

[0078] Figure 24 This is a schematic diagram of the suction component of the present invention.

[0079] In the picture:

[0080] 1. Base; 2. Barrel body; 3. Feeding pipe; 4. Hollow top cover; 5. Wavemaker pump; 6. Suction device; 7. Heating rod; 8. Ultraviolet lamp; 9. Cover plate; 10. Mounting ring; 11. Mounting port; 12. Sampling pipe; 13. Fixing pipe; 14. Rotating pipe; 15. Rotating gear; 16. Hollow boss; 17. Rotating ring; 18. Sampling hole; 19. Suction channel; 20. Drainage channel; 21. Connecting hole; 22. Suction pipe; 23. Insertion hole;

[0081] 24. Drive assembly; 25. Blocking assembly; 26. Sampling assembly; 27. Power slot; 28. Power gear;

[0082] 29. Drive motor; 30. Drive slot; 31. Drive ring; 32. Slot; 33. Sleeve; 34. Drainage channel; 35. Connecting interface; 36. Sealing ball; 37. Push rod; 38. Sampling port; 39. Connecting tube; 40. Return spring; 41. Plug; 42. Fixing bracket; 43. Tube rack; 44. Sampling tube; 45. Sliding groove; 46. Baffle plate; 47. Buckle ring; 48. Hook; 49. Control component; 50. Suction assembly; 51. Push rod; 52. Push plate; 53. Push threaded rod; 54. Pressure plate; 55. Suction port; 56. Moving slide; 57. Baffle plate; 58. Slot; 59. Rotating shaft; 60. Pull rod; 61. Paddle plate. Detailed Implementation

[0083] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings.

[0084] Example 1, referring to Figure 1-16The first embodiment of the present invention provides a probiotic fermentation and amplification device, including a base 1, a barrel 2 disposed on the top of the base 1, a feeding pipe 3 disposed on the outside of the barrel 2, a hollow top cover 4 disposed on the top of the barrel 2, a wave-making pump 5 disposed on the inside of the base 1, a cover plate 9 disposed on the top of the hollow top cover 4, a suction device 6 disposed on the top of the cover plate 9, a heating rod 7 and an ultraviolet lamp 8 disposed on the bottom of the hollow top cover 4, and also includes a sampling unit for collecting probiotic fermentation liquid samples in the barrel 2;

[0085] Specifically, the base 1 and the barrel 2 are connected by bolts, the bottom of the base 1 is equipped with a drain pipe, and the power and wave direction of the wave pump 5 can be adjusted.

[0086] The sampling unit includes a mounting ring 10 disposed between the barrel body 2 and the hollow top cover 4, a mounting port 11 disposed at the top of the mounting ring 10, a sampling pipe 12 disposed inside the mounting port 11, a hollow boss 16 disposed at the bottom inner side of the hollow top cover 4, a rotating ring 17 disposed inside the hollow top cover 4, a sampling hole 18 disposed on the surface of the rotating ring 17, a suction channel 19 and a drainage channel 20 disposed between the inner ring wall of the rotating ring 17 and the sampling hole 18, a docking hole 21 disposed on the outer side of the hollow boss 16, a suction pipe 22 disposed on the outer side of the suction device 6, and an insertion hole 23 disposed at the bottom of the rotating gear 15.

[0087] Specifically, the mounting ring 10 is fixedly mounted on the top of 2 by bolts, the hollow top cover 4 is fixedly mounted on the top of the mounting ring 10 by bolts, the rotating ring 17 is rotatably connected to the outside of the hollow boss 16, the sampling holes 18 are distributed in a ring array with the central axis of the rotating ring 17 as the reference, the number of sampling holes 18 is an integer multiple of the number of sampling pipes 12, there are four sampling pipes 12, and four sets of sampling holes 18 are also set accordingly, the volume of one set of sampling holes 18 is the same as the volume of sampling pipes 12, the drain channel 20 extends into the interior of the hollow boss 16, the drain channel 20 is provided with a one-way flap, the docking hole 21 is provided with the sampling pipe 12, the docking hole 21 is docked with the suction channel 19, the bottom end of the suction tube 22 penetrates downward into the interior of the hollow boss 16, the bottom of the rotating ring 17 is provided with a sealing gasket, the top end of the sampling pipe 12 extends into the insertion hole 23, the sampling pipe 12 is slidably inserted into the mounting port 11, and the sampling pipes 12 are distributed in a ring array around the central axis of the mounting ring 10;

[0088] The sampling unit also includes a driving component 24 and a blocking component 25. The driving component 24 is used to provide driving force for the rotating ring 17 and to selectively block the suction channel 19.

[0089] The above sampling scheme, through the cooperation of sampling pipe 12 and sampling hole 18, when sampling is required, the suction device 6 generates suction force, which is transmitted to the sampling hole 18. The fermentation liquid in the sampling pipe 12 is sucked into the sampling hole 18. The drive component 24 drives the rotating ring 17 to rotate and switch the sampling hole 18 connected to the sampling pipe 12. The fermentation liquid in the sampling pipe 12 is sucked into another sampling hole 18. Different sampling holes 18 hold different layers of fermentation liquid in the sampling pipe 12. In this way, fermentation liquid samples from various areas can be obtained. The operator can make a more accurate judgment on the fermentation liquid in the tank 2. At the same time, abnormal fermentation areas can be quickly identified, and the fermentation situation in abnormal areas can be improved by adjusting the wave pump 5 and the heating rod 7.

[0090] Reference Figure 9-10 The drive assembly 24 includes a power groove 27 disposed on the top of the rotating ring 17, a power gear 28 disposed on the bottom of the cover plate 9, and a drive motor 29 disposed on the top of the cover plate 9.

[0091] Specifically, the inner wall of the power groove 27 is provided with teeth, the power gear 28 is located inside the power groove 27, the outer teeth of the power gear 28 are only half a circle, the power gear 28 meshes with the teeth on the inner wall of the power groove 27, and the power gear 28 is connected to the power output shaft of the drive motor 29.

[0092] By using the above-mentioned scheme, a power gear 28 with only half a turn of teeth is used. When the teeth of the power gear 28 mesh with the power groove 27, the rotating ring 17 rotates. When the teeth separate, the rotating ring 17 stops, allowing the sampling hole 18 sufficient time to draw the fermentation liquid.

[0093] Reference Figure 10-13 The drive assembly 24 also includes a drive groove 30 disposed at the bottom of the inner side of the hollow top cover 4, and a drive ring 31 disposed at the bottom of the rotating ring 17.

[0094] Specifically, the sampling pipe 12 includes a fixed pipe 13 and a rotating pipe 14. Both the fixed pipe 13 and the rotating pipe 14 are semi-circular pipes. Both the fixed pipe 13 and the rotating pipe 14 are inserted into the mounting port 11. The rotating pipe 14 is rotatably connected to the inner wall of the fixed pipe 13. A rotating gear 15 is provided on the outer side of the rotating pipe 14. The drive groove 30 is connected to the insertion hole 23. The drive ring 31 is rotatably connected inside the drive ring 31. The inner ring surface of the drive ring 31 is provided with teeth, and the length of the teeth is half the circumference of the rotating gear 15. The number of teeth on the inner ring surface of the drive ring 31 is the same as the number of sampling pipes 12. The rotating gear 15 meshes with the teeth on the inner ring surface of the drive ring 31.

[0095] The above sampling scheme involves setting up a sampling pipe 12. When the rotating ring 17 rotates, it drives the rotating tube 14 to rotate via the driving ring 31, so that the fixed tube 13 and the rotating tube 14 are combined into a complete pipe. During aspiration, the fermentation liquid in the pipe enters the sampling hole 18 little by little from top to bottom. After sampling is completed, the rotating ring 17 rotates, and the driving ring 31 drives the rotating tube 14 to rotate. The rotating tube 14 rotates into the fixed tube 13, so that the sampling pipe 12 is fully open. The fermentation liquid in the sampling pipe 12 interacts with the fermentation liquid in various areas of the barrel 2, so that the sample obtained by the sampling pipe 12 is more comprehensive. At the same time, after the sampling pipe 12 is opened, its pressure is limited, and the fermentation liquid above the fermentation liquid level in the barrel 2 flows back into the barrel 2. As the rotating ring 17 rotates, the fermentation liquid in each sampling hole 18 is emptied, ensuring the accuracy of continuous sampling.

[0096] Reference Figure 15-16 The blocking assembly 25 includes a slot 32 disposed on the top of the rotating ring 17, a sleeve 33 disposed inside the slot 32, a drain channel 34 disposed between the slot 32 and the sampling hole 18, a mating interface 35 disposed on the outside of the sleeve 33, a blocking ball 36 disposed inside the sleeve 33, and a push rod 37 disposed on the top of the sleeve 33.

[0097] Specifically, slot 32 intersects with suction channel 19, sleeve 33 cuts off suction channel 19, discharge channel 34 penetrates into sleeve 33, interface 35 is connected to suction channel 19, and the material density of sealing ball 36 is less than the density of fermentation liquid.

[0098] By setting up the above sampling scheme and blocking component 25, as the suction continues, the amount of fermentation liquid in the sampling hole 18 increases. After the fermentation liquid enters the suction channel 19, it flows into the sleeve 33. The sealing ball 36 floats up and blocks the interface 35, thus blocking the suction channel 19 and preventing the fermentation liquid from entering the hollow boss 16, ensuring the stability of continuous sampling.

[0099] During use, when sampling, the suction device 6 and the drive motor 29 are turned on simultaneously. The suction device 6 creates a negative pressure environment inside the hollow boss 16 through the suction tube 22. Since the sampling hole 18 and the hollow boss 16 are connected through the suction channel 19 and the docking hole 21, the negative pressure inside the hollow boss 16 acts on the sampling hole 18. The drive motor 29 drives the rotating ring 17 to rotate clockwise through the power gear 28. The drive ring 31 follows the rotation of the rotating ring 17. The drive ring 31 drives the rotating tube 14 to rotate through the rotating gear 15 (at this time, when the rotating tube 14 rotates and contacts the deflector 61, the deflector 61 deflects to one side to avoid the rotating tube 14 and is not affected by the rotating tube 14), so that the fixed tube 13 and the rotating tube 14 form a complete pipeline. Figure 6As shown, the teeth of the drive ring 31 then separate from the rotating gear 15, the rotating tube 14 stops rotating, and when the rotating ring 17 rotates to connect the sampling hole 18 with the insertion hole 23, the fermentation liquid in the sampling pipe 12 flows from bottom to top under the influence of negative pressure and enters the sampling hole 18 little by little to obtain a sample of a region.

[0100] The fermentation liquid in the sampling hole 18 increases continuously until it flows into the suction channel 19. When the fermentation liquid passes through the sleeve 33 in the suction channel 19, it flows into the sleeve 33. The sealing ball 36 in the sleeve 33 is lifted by the buoyancy of the fermentation liquid. When the sealing ball 36 floats to the interface 35, it will block the interface 35. The suction channel 19 is blocked by the sleeve 33, preventing the fermentation liquid from flowing into the hollow boss 16. The rotating ring 17 continues to rotate, so that another sampling hole 18 is connected to the insertion hole 23, and a sample of another area is obtained. In this way, the rotating ring 17 continues to rotate, and different sampling holes 18 are connected to the insertion hole 23 one by one to obtain samples of multiple areas.

[0101] Example 2, refer to Figure 17-21 This is the second embodiment of the present invention, which differs from the first embodiment in that the sampling unit further includes a sampling component 26 for taking out the fermentation broth sample;

[0102] The sampling assembly 26 includes a sampling port 38 disposed on the top of the cover plate 9, a connecting tube 39 disposed inside the sampling port 38, a return spring 40 disposed between the outer wall of the connecting tube 39 and the inner wall of the sampling port 38, a plug 41 disposed on the top of the connecting tube 39, a fixing bracket 42 disposed on the top of the cover plate 9, a tube rack 43 disposed on the top of the fixing bracket 42, and a sampling tube 44 disposed on the top of the tube rack 43.

[0103] Specifically, the sampling port 38 penetrates the cover plate 9 and its distribution position corresponds to the sampling hole 18. The sampling tube 44 penetrates the tube frame 43 and its distribution position corresponds to the sampling port 38. The connection part between the fixing frame 42 and the tube frame 43 is a sampling telescopic structure. The telescopic structure is equipped with an elastic component. The lower end of the sampling tube 44 is equipped with a rubber diaphragm. The sampling tube 44 needs to be vacuumed when in use. The sampling tube 44 is snapped onto the tube frame 43. The surface of the plug 41 is provided with a through hole that communicates with the connecting tube 39.

[0104] The above sampling scheme is implemented by setting up the sampling component 26. After all the sampling holes 18 are filled with fermentation broth samples, the tube rack 43 is pressed, and the sampling tube 44 moves downward to contact the plug 41. The plug 41 is inserted into the push rod 51, and the connecting tube 39 moves downward into the sampling hole 18. The sampling tube 44 draws out the fermentation broth in the sampling hole 18, thus completing the sampling.

[0105] The sampling assembly 26 also includes a sliding groove 45 disposed on the top of the cover plate 9, a baffle plate 46 disposed inside the sliding groove 45, a buckle 47 disposed on the surface of the baffle plate 46 and the tube rack 43, and a hook 48 disposed between the two buckles 47.

[0106] Specifically, the sliding groove 45 covers the upper opening of the sampling port 38, and the diameter of the push rod 37 is smaller than the diameter of the sleeve 33 of the interface 35.

[0107] When the tube rack 43 is pressed down, the shield 46 is pushed open by the hook 48, exposing the connecting tube 39 and plug 41 inside the sampling port 38. When the tube rack 43 is reset upward, the hook 48 pulls the shield 46 back, sealing the sampling port 38, reducing dust and bacteria from entering the sampling port 38, and preventing the fermentation broth sample from being contaminated.

[0108] During use, after sampling is completed, the sampling tube 44 is inserted into the tube rack 43, and then the tube rack 43 is pressed. The fixing bracket 42 is compressed and moves downward. When the tube rack 43 moves downward, the buckle 47 on the tube rack 43 approaches the buckle 47 on the baffle plate 46. The tube rack 43 pushes the baffle plate 46 away from the sampling port 38 through the hook 48. The sampling tube 44 first contacts the plug 41. The plug 41 and the connecting tube 39 are pushed downward, and the connecting tube 39 enters the sampling hole 1. After the sampling tube 44 comes into contact with the fermentation broth and the connecting tube 39 can no longer move, the sampling tube 44 continues to move downwards. The plug 41 is inserted into the rubber diaphragm below the sampling tube 44. The fermentation broth in the sampling hole 18 is sucked into the sampling tube 44 through the connecting tube 39 and the plug 41. The tube rack 43 is released, and the telescopic structure pushes the tube rack 43 back to its original position. The tube rack 43 moves upwards, and the baffle plate 46 is pulled by the hook 48 to close the sampling port 38. The sampling tube 44 is then removed from the tube rack 43 and tested separately.

[0109] The remaining structure is the same as that in Example 1.

[0110] Example 3, referring to Figure 17-24 This is the third embodiment of the present invention. The difference between this embodiment and the second embodiment is that a finished product collection unit is also provided on the fixed tube 13 and the cover plate 9 for acquiring the fermentation broth after amplification.

[0111] The finished product collection unit includes a control component 49 located on the top of the cover plate 9, which is used to control the opening and closing of the suction channel 19;

[0112] The control assembly 49 includes a push rod 51 disposed on the top of the cover plate 9, a push plate 52 disposed on the top of the push rod 51, a push thread rod 53 disposed on the top of the push plate 52, and a pressure plate 54 disposed at the bottom of the push rod 51.

[0113] The push rod 51 is slidably inserted above the cover plate 9, and the lower end of the push rod 51 extends to the bottom of the cover plate 9. The pressure plate 54 is located above the top rod 37. The push thread rod 53 passes through the push plate 52. The push plate 52 is rotatably connected to the push plate 52, and the push plate 52 is threadedly connected to the cover plate 9.

[0114] Using the above sampling method, by setting up the control component 49, when a fermentation broth sample is needed, the push threaded rod 53 is rotated, and the push plate 52 presses down on the pressure plate 54 through the push insertion rod 51. The pressure plate 54 presses the top rod 37 into the sleeve 33, and the top rod 37 prevents the sealing ball 36 from floating up. This ensures that the suction channel 19 is always unobstructed, and multiple sampling pipes 12 collect fermentation broth, which is then collected in the hollow boss 16, making the quality of each fermentation broth sample taken more uniform.

[0115] The finished product collection unit also includes a separation and suction component 50 located on the outside of the fixed tube 13, which is used to collect fermentation broth at different depths;

[0116] The suction assembly 50 includes a suction port 55 disposed on the outside of the fixed tube 13, a movable slide groove 56 disposed on the surface of the fixed tube 13, a baffle 57 disposed on the surface of the suction port 55, a slot 58 disposed on the side of the baffle 57 near the suction port 55, a rotating shaft 59 disposed inside the suction port 55, a pull rod 60 disposed on the outside of the rotating shaft 59, and a lever 61 disposed on the end of the pull rod 60 away from the baffle 57.

[0117] The movable chute 56 is distributed on both sides of the suction port 55. The cover plate 57 is slidably connected to the movable chute 56. The material density of the cover plate 57 is less than that of the fermentation liquid. A torsion spring 1 is set between the pull rod 60 and the rotating shaft 59. A torsion spring 2 is set between the pull rod 60 and the lever 61. The force required for the torsion spring 2 to stretch is less than that required for the torsion spring 1 to stretch. The lever 61 can only deflect in one direction. The lever 61 extends to the inside of the fixed tube 13.

[0118] The above sampling scheme involves setting up a sampling assembly 50. During sampling, the rotating tube 14 rotates clockwise, contacting the deflector 61. The deflector 61 deflects, while the shield 57 remains unaffected, always keeping the suction port 55 closed. When using the fermentation broth sample, the rotating tube 14 rotates counterclockwise, contacting the deflector 61. The deflector 61 drives the pull rod 60 to deflect, causing the pull rod 60 to shift out of the slot 58. At this time, the shield 57, located in the fermentation broth, floats up, and the suction port 55 opens, allowing the sampling pipe 12 to draw fermentation broth from different areas within the barrel 2. This results in a more balanced quality of the drawn fermentation broth. As the fermentation broth continues to decrease, the shield 57 above the fermentation broth loses buoyancy and slides down to seal the suction port 55, preventing air from entering the sampling pipe 12 during suction. This makes the suction process more stable and efficient.

[0119] During use, when fermentation amplification occurs and fermentation broth needs to be extracted, the suction device 6 and drive motor 29 are activated simultaneously. The suction device 6 creates a negative pressure environment within the hollow boss 16 through the suction tube 22. The drive motor 29 drives the rotating ring 17 to rotate counterclockwise through the power gear 28. The drive ring 31 follows the rotating ring 17 and drives the rotating tube 14 to rotate counterclockwise through the rotating gear 15. The rotating tube 14 drives the pull rod 60 to deflect, causing the pull rod 60 to disengage from the slot 58 and lose its restraint. At this time, the baffle 57 located in the fermentation broth floats up, opening the suction port 55, allowing the sampling pipe 12 to extract fermentation broth from different locations. As the fermentation broth decreases, the baffle 57 above the fermentation broth loses its buoyancy support and slides down to block the suction port 55. The suction continues. When the sampling pipe 12 can no longer extract fermentation broth, the drain pipe is opened to discharge the fermentation broth residue.

[0120] The remaining structure is the same as that in Example 2.

[0121] Example 4, refer to Figure 1-24 The fourth embodiment of the present invention provides: a method for probiotic fermentation and amplification, comprising the following steps:

[0122] Step 1: Preparation: First, clean the entire set of equipment with tap water to keep it clean. Move the equipment to a room equipped with ultraviolet lamps. Then, install the ultraviolet lamp 8, heating rod 7 and wave pump 5 onto the base 1 and hollow top cover 4. Adjust the heating rod 7 to a constant temperature of 35℃.

[0123] Step 2: Prepare the solution: Add brown sugar and water to container 2 in a ratio of 1:10;

[0124] Step 3; Sterilization: Turn on wave pump 5, UV lamp 8 and heating rod 7. Use UV lamp 8 to disinfect for 4 hours. Keep the water temperature at 35℃. The tank wall should feel warm to the touch.

[0125] Step 4: Inoculation and Cultivation: After sterilization, turn off the UV lamp 8 inside the tank 2, add probiotics according to the ratio, cover with the hollow top lid 4 to keep it sealed, and ferment at a constant temperature for 48 hours. Maintain the temperature at around 35℃, keep the wave generator pump 5 running continuously during fermentation, keep the UV lamps in the room on all the time, and turn them off when personnel are working;

[0126] Step 5: Regularly check the equipment operation, maintain a constant temperature, ensure the wave generator pump 5 is running normally, and incubate for 48 hours (if gas is produced, make a small hole in the lid and insert a vent tube, and fill it with sterile cotton; during fermentation, place it in a cool place).

[0127] Step 6: After successful amplification, turn off wave pump 5 and heating rod 7, and take out the fermentation liquid in batches according to the product usage recommendation. The fermentation liquid must be used up within 7 days. When taking out the liquid multiple times, keep the hollow top cover 4 sealed.

[0128] Step 7: After using the fermentation liquid, thoroughly rinse the base 1, tank 2, heating rod 7, wavemaker pump 5, UV lamp 8, etc., with tap water. Ensure there are no foreign objects, impurities, or odors remaining for future use. After cleaning, irradiate with UV lamp 8 for 10-30 minutes.

[0129] By sampling the above scheme and using the new fermentation process, the quality of probiotics is better and the amplification efficiency is higher.

[0130] The usage methods of the equipment involved in Examples 1-3 are as follows:

[0131] During sampling, the suction device 6 and the drive motor 29 are turned on simultaneously. The suction device 6 creates a negative pressure environment inside the hollow boss 16 through the suction tube 22. Since the sampling hole 18 and the hollow boss 16 are connected through the suction channel 19 and the docking hole 21, the negative pressure inside the hollow boss 16 acts on the sampling hole 18. The drive motor 29 drives the rotating ring 17 to rotate clockwise through the power gear 28. The drive ring 31 follows the rotation of the rotating ring 17. The drive ring 31 drives the rotating tube 14 to rotate through the rotating gear 15 (at this time, when the rotating tube 14 rotates and contacts the deflector 61, the deflector 61 deflects to one side to avoid the rotating tube 14 and is not affected by the rotating tube 14), so that the fixed tube 13 and the rotating tube 14 form a complete pipeline. Figure 6 As shown, the teeth of the drive ring 31 then separate from the rotating gear 15, the rotating tube 14 stops rotating, and when the rotating ring 17 rotates to connect the sampling hole 18 with the insertion hole 23, the fermentation liquid in the sampling pipe 12 flows from bottom to top under the influence of negative pressure and enters the sampling hole 18 little by little to obtain a sample of a region.

[0132] The fermentation liquid in the sampling hole 18 increases continuously until it flows into the suction channel 19. When the fermentation liquid passes through the sleeve 33 in the suction channel 19, it flows into the sleeve 33. The sealing ball 36 in the sleeve 33 is lifted by the buoyancy of the fermentation liquid. When the sealing ball 36 floats to the interface 35, it will block the interface 35. The suction channel 19 is blocked by the sleeve 33, preventing the fermentation liquid from flowing into the hollow boss 16. The rotating ring 17 continues to rotate, so that another sampling hole 18 is connected to the insertion hole 23, and a sample of another area is obtained. In this way, the rotating ring 17 rotates continuously, and different sampling holes 18 are connected to the insertion hole 23 one by one to obtain samples of multiple areas.

[0133] After sampling, the sampling tube 44 is inserted into the tube rack 43. Then, the tube rack 43 is pressed, and the fixing frame 42 is compressed and moved downward. When the tube rack 43 moves downward, the buckle 47 on the tube rack 43 approaches the buckle 47 on the baffle plate 46. The tube rack 43 pushes the baffle plate 46 away from the sampling port 38 through the hook 48. The sampling tube 44 first contacts the plug 41. The plug 41 and the connecting tube 39 are pushed downward. The connecting tube 39 enters the sampling hole 18 and contacts the fermentation liquid. After the connecting tube 39 can no longer move, the sampling tube 44 continues to move downward. The plug 41 is inserted into the rubber diaphragm below the sampling tube 44. The fermentation liquid in the sampling hole 18 is sucked into the sampling tube 44 through the connecting tube 39 and the plug 41. The tube rack 43 is released, and the telescopic structure pushes the tube rack 43 back to its original position. The tube rack 43 moves upward and pulls the baffle plate 46 through the hook 48 to close the sampling port 38. The sampling tube 44 is removed from the tube rack 43 and tested separately.

[0134] After sampling is completed, the rotating ring 17 continues to rotate, and the driving ring 31 drives the rotating tube 14 to rotate again through the teeth, causing the rotating tube 14 to rotate into the fixed tube 13, as shown. Figure 7 As shown, the sampling pipe 12 is open on the side, and the fermentation liquid in the barrel 2 can flow into the sampling pipe 12 from the side. At the same time, after the sampling pipe 12 is opened, the pressure restriction is lost, and the fermentation liquid that is higher than the surface of the fermentation liquid in the barrel 2 flows back into the barrel 2. As the rotating ring 17 rotates, the fermentation liquid in each sampling hole 18 is drained.

[0135] When fermentation amplification occurs and fermentation broth needs to be extracted, the suction device 6 and drive motor 29 are activated simultaneously. The suction device 6 creates a negative pressure environment within the hollow boss 16 through the suction tube 22. The drive motor 29 drives the rotating ring 17 to rotate counterclockwise through the power gear 28. The drive ring 31 follows the rotating ring 17 and drives the rotating tube 14 to rotate counterclockwise through the rotating gear 15. The rotating tube 14 drives the pull rod 60 to deflect, causing the pull rod 60 to disengage from the slot 58 and lose its restraint. At this time, the baffle 57 located in the fermentation broth floats up, opening the suction port 55, allowing the sampling pipe 12 to extract fermentation broth from different locations. As the fermentation broth decreases, the baffle 57 above the fermentation broth loses its buoyancy support and slides down to block the suction port 55. The suction continues. When the sampling pipe 12 can no longer extract fermentation broth, the drain pipe is opened to discharge the fermentation broth residue.

[0136] It should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention and are not intended to limit it. Although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art should understand that modifications or equivalent substitutions can be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention, and all such modifications or substitutions should be covered within the scope of the claims of the present invention.

Claims

1. A probiotic fermentation and amplification device, comprising a base (1), a barrel (2) disposed on the top of the base (1), a feeding pipe (3) disposed on the outside of the barrel (2), a hollow top cover (4) disposed on the top of the barrel (2), a wave-generating pump (5) disposed on the inside of the base (1), a cover plate (9) disposed on the top of the hollow top cover (4), a suction device (6) disposed on the top of the cover plate (9), a heating rod (7) disposed on the bottom of the hollow top cover (4), and an ultraviolet lamp (8), characterized in that: It also includes a sampling unit for collecting fermentation liquid samples inside the barrel (2); The sampling unit includes a mounting ring (10) disposed between the barrel body (2) and the hollow top cover (4), a mounting port (11) disposed at the top of the mounting ring (10), a sampling pipe (12) disposed inside the mounting port (11), a hollow boss (16) disposed at the bottom of the inner side of the hollow top cover (4), a rotating ring (17) disposed inside the hollow top cover (4), a sampling hole (18) disposed on the surface of the rotating ring (17), a suction channel (19) and a drainage channel (20) disposed between the inner ring wall of the rotating ring (17) and the sampling hole (18), a docking hole (21) disposed on the outer side of the hollow boss (16), a suction pipe (22) disposed on the outer side of the suction device (6), and an insertion hole (23) disposed at the bottom of the rotating gear (15). The sampling holes (18) are arranged in a ring array with the central axis of the rotating ring (17) as the reference. The drainage channel (20) extends into the hollow boss (16). The docking hole (21) and the suction channel (19) are docked. The bottom end of the suction tube (22) extends downward into the hollow boss (16). The top end of the sampling pipe (12) extends into the insertion hole (23). The sampling unit further includes a driving component (24) and a blocking component (25). The driving component (24) is used to provide driving force to the rotating ring (17) and to selectively block the suction channel (19).

2. The probiotic fermentation and amplification equipment according to claim 1, characterized in that: The drive assembly (24) includes a power groove (27) disposed on the top of the rotating ring (17), a power gear (28) disposed on the bottom of the cover plate (9), and a drive motor (29) disposed on the top of the cover plate (9). The inner wall of the power groove (27) is provided with teeth, the power gear (28) is located in the power groove (27), the outer teeth of the power gear (28) are only half a circle, and the power gear (28) meshes with the teeth of the inner wall of the power groove (27).

3. The probiotic fermentation and amplification equipment according to claim 2, characterized in that: The drive assembly (24) also includes a drive groove (30) disposed at the bottom of the inner side of the hollow top cover (4) and a drive ring (31) disposed at the bottom of the rotating ring (17); The sampling pipe (12) includes a fixed pipe (13) and a rotating pipe (14). Both the fixed pipe (13) and the rotating pipe (14) are semi-circular pipes. Both the fixed pipe (13) and the rotating pipe (14) are inserted into the mounting port (11). The rotating pipe (14) is rotatably connected to the inner wall of the fixed pipe (13). A rotating gear (15) is provided on the outer side of the rotating pipe (14). The drive groove (30) is connected to the insertion hole (23). The drive ring (31) is rotatably connected to the drive ring (31). The inner ring surface of the drive ring (31) is provided with teeth. The rotating gear (15) meshes with the teeth on the inner ring surface of the drive ring (31).

4. The probiotic fermentation and amplification equipment according to claim 3, characterized in that: The blocking assembly (25) includes a slot (32) disposed on the top of the rotating ring (17), a sleeve (33) disposed inside the slot (32), a drain channel (34) disposed between the slot (32) and the sampling hole (18), a mating interface (35) disposed on the outside of the sleeve (33), a blocking ball (36) disposed inside the sleeve (33), and a top rod (37) disposed on the top of the sleeve (33). The slot (32) intersects with the suction channel (19), the sleeve (33) cuts off the suction channel (19), the discharge channel (34) penetrates into the sleeve (33), the interface (35) is connected to the suction channel (19), and the material density of the sealing ball (36) is less than the density of the fermentation liquid.

5. The probiotic fermentation and amplification equipment according to claim 4, characterized in that: The sampling unit further includes a sampling component (26) for extracting fermentation broth samples; The sampling assembly (26) includes a sampling port (38) disposed on the top of the cover plate (9), a connecting tube (39) disposed inside the sampling port (38), a return spring (40) disposed between the outer wall of the connecting tube (39) and the inner wall of the sampling port (38), a plug (41) disposed on the top of the connecting tube (39), a fixing bracket (42) disposed on the top of the cover plate (9), a tube rack (43) disposed on the top of the fixing bracket (42), and a sampling tube (44) disposed on the top of the tube rack (43). The sampling port (38) passes through the cover plate (9) and its distribution position corresponds to the sampling hole (18). The sampling tube (44) passes through the tube rack (43) and its distribution position corresponds to the sampling port (38).

6. The probiotic fermentation and amplification equipment according to claim 5, characterized in that: The sampling assembly (26) also includes a sliding groove (45) disposed on the top of the cover plate (9), a baffle plate (46) disposed inside the sliding groove (45), a buckle (47) disposed on the surface of the baffle plate (46) and the tube rack (43), and a hook (48) disposed between the two buckles (47). The sliding groove (45) covers the upper opening of the sampling port (38), and the diameter of the push rod (37) is smaller than the diameter of the sleeve (33) of the interface (35).

7. The probiotic fermentation and amplification equipment according to claim 6, characterized in that: The fixed tube (13) and the cover plate (9) are also equipped with a finished product collection unit for obtaining the fermentation broth after amplification; The finished product collection unit includes a control component (49) disposed on the top of the cover plate (9) for controlling the opening and closing of the suction channel (19); The control component (49) includes a push rod (51) disposed on the top of the cover plate (9), a push plate (52) disposed on the top of the push rod (51), a push thread rod (53) disposed on the top of the push plate (52), and a pressure plate (54) disposed on the bottom of the push rod (51). The push rod (51) is slidably inserted above the cover plate (9), the lower end of the push rod (51) extends to the bottom of the cover plate (9), the pressure plate (54) is located above the top rod (37), the push thread rod (53) passes through the push plate (52), the push plate (52) is rotatably connected to the push plate (52), and the push plate (52) is threadedly connected to the cover plate (9).

8. The probiotic fermentation and amplification equipment according to claim 7, characterized in that: The finished product collection unit also includes a separation and absorption component (50) disposed on the outside of the fixed tube (13) for collecting fermentation broth at different depths; The suction assembly (50) includes a suction port (55) disposed on the outside of the fixed tube (13), a movable slide groove (56) disposed on the surface of the fixed tube (13), a baffle (57) disposed on the surface of the suction port (55), a slot (58) disposed on the side of the baffle (57) near the suction port (55), a rotating shaft (59) disposed inside the suction port (55), a pull rod (60) disposed on the outside of the rotating shaft (59), and a lever (61) disposed on the end of the pull rod (60) away from the baffle (57). The movable chute (56) is distributed on both sides of the suction port (55). The baffle (57) is slidably connected to the movable chute (56). The material density of the baffle (57) is less than that of the fermentation liquid. A torsion spring is provided between the pull rod (60) and the rotating shaft (59), and a torsion spring is provided between the pull rod (60) and the lever (61).

9. A method for probiotic fermentation and amplification, comprising sampling the probiotic fermentation and amplification equipment as described in claim 8, characterized in that, Includes the following steps: Step 1: Preparation: First, clean the entire set of equipment with tap water to keep the equipment clean. Move the equipment to a room equipped with ultraviolet lamps. Then install the ultraviolet lamp (8), heating rod (7) and wave pump (5) onto the base (1) and hollow top cover (4). Adjust the heating rod (7) to a constant temperature of 35℃. Step 2: Prepare the solution: Add brown sugar and water to the barrel (2) in the correct proportion; Step 3; Sterilization: Turn on the wave pump (5), ultraviolet lamp (8) and heating rod (7), disinfect with ultraviolet lamp (8) for 4 hours, and keep the water temperature at 35℃; Step 4: Inoculation and cultivation: After sterilization, turn off the ultraviolet lamp (8) inside the barrel (2), add probiotics in proportion, cover with the hollow top cover (4), and ferment at a constant temperature for 48 hours; Step 5: Regularly check the equipment operation, maintain a constant temperature, ensure the wave generator (5) is running normally, and incubate for 48 hours; Step 6: After successful amplification, turn off the wave generator (5) and heating rod (7), and remove them for use in batches; Step 7: After the fermentation liquid is used up, the base (1), barrel (2), heating rod (7), wave pump (5), ultraviolet lamp (8), etc. should be rinsed with tap water.

10. The probiotic fermentation and amplification method according to claim 9, characterized in that: In step four, the wave generator pump (5) is kept running continuously during the fermentation process.

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