Intermittent inoculation track control device capable of controlling quantitative change of liquid strains

Abstract

The invention discloses an intermittent inoculation track control device for controllable quantitative change of liquid strains, and relates to the technical field of track control of the liquid strains in fungus bags, a fungus conveying pipe assembly is connected with two ends of a fungus supply pipe and two ends of a liquid storage pipe respectively, and a fungus outlet pipe assembly is connected with two ends of an inoculator, a bag surface inoculation structure and two ends of the liquid storage pipe respectively; the pressure of the liquid storage pipe can be adjusted according to a spacing set value, when the number of the bacterium injection structures is larger than one, left-right interchange type work is achieved, liquid strains are conveyed into the inoculator and the bag surface inoculation structure through the bacterium outlet pipe assembly, and the bag surface inoculation structure comprises a plurality of control units arranged side by side; the control unit can be independently opened and closed according to inoculation parameter setting and inoculation track form setting and is used for controlling the forms of inoculation tracks of the inner layer and the outer layer of the base material bag surface in the fungus bag. According to the method, the inoculation amount and the inoculation track of the liquid strain can be controlled, the situation that the inoculation amount is too large is avoided, and the comprehensive input cost of a fungus bag is effectively reduced while the original inoculation effect is not affected.

Description

Technical Field

[0001] This invention relates to the field of inoculation trajectory control technology for liquid spawn bulk technology of edible fungi spawn bags (the substrate is sterilized before bagging, and inoculation is carried out simultaneously during bagging), and in particular to a discontinuous inoculation trajectory control device for controllable quantitative change of liquid spawn. Background Technology

[0002] There are currently two methods for inoculating traditional edible mushroom spawn bags with liquid spawn: 1. Single-sided inoculation: Traditional edible mushroom bags use a production process of first packing the substrate, then sterilizing, cooling, and finally inoculating.

[0003] Disadvantages: The inoculation point is singular, and can only be inoculated on the surface of the substrate inside the bag or in the pores of the substrate. There are few contact points for the spawn, and the mycelium is in a unidirectional growth trajectory, which leads to a long inoculation period, inconsistent mycelial age inside the substrate, and a high contamination rate. The inoculation period of the spawn bag is generally 25-40 days for most varieties, while for some rare varieties such as red-topped bamboo fungus, the inoculation period can be as long as 90-120 days for the mycelium to fully colonize the bag.

[0004] 2. Bulk Inoculation: The existing bulk briquette manufacturing process adopts a production process in which the base material is first sterilized in a fully enclosed sterilization chamber, then cooled in a closed cooling chamber, and finally packaged and inoculated simultaneously.

[0005] Advantages: The large contact surface of the spawn and the bidirectional growth of the mycelium can greatly shorten the cultivation cycle of the spawn bags. There is almost no contamination. For general varieties, the mycelium can be fully cultivated in just 12 days using the bulk material sterilization process.

[0006] The two inoculation methods mentioned above share a common feature: the liquid inoculation process of a single spawn bag is completed in one go. In particular, during the inoculation of spawn bags made from existing bulk materials, the output liquid spawn leaves complete and unbroken inoculation tracks in the center and outer surface of the substrate inside the spawn bag. After the inoculation program is set, the inoculation amount of each spawn bag is basically consistent and fixed. The existing inoculation effect lacks the ability to control the inoculation program for liquid spawn, the deformation of the inoculation track, and equivalent variables. Summary of the Invention

[0007] The purpose of this invention is to provide a controllable, intermittent inoculation trajectory control device for liquid microbial culture, in order to solve the problems existing in the prior art, so that the inoculation amount and inoculation trajectory pattern of the liquid microbial culture can be controlled, avoiding excessive inoculation amount, and effectively reducing the overall input cost of the microbial culture bag without affecting the original inoculation effect.

[0008] To achieve the above objectives, the present invention provides the following solution: This invention provides a controllable, intermittent inoculation trajectory control device for liquid microbial culture, comprising an inoculation structure, a bag surface inoculation structure, and at least one injection structure. The inoculation structure has an inlet for introducing liquid microbial culture, and its outlet is connected to each injection structure. Each injection structure includes a supply tube, a storage tube, a delivery tube assembly, and an outlet tube assembly. The supply tube is used to introduce liquid microbial culture. The delivery tube assembly is connected to both ends of the supply tube and the storage tube. The outlet tube assembly is connected to both ends of the inoculator, the bag surface inoculation structure, and the storage tube. The pressure of the storage tube is adjustable according to a set interval value. When there is more than one injection structure, the injection structures operate in a left-right interchangeable manner, delivering liquid microbial culture to the inoculator and the bag surface inoculation structure via the outlet tube assembly. The bag surface inoculation structure includes multiple parallel control units, which can be individually opened and closed according to inoculation parameters and inoculation trajectory settings to control the inoculation trajectory shape of the inner and outer layers of the substrate bag surface inside the liquid microbial culture.

[0009] Preferably, the infusion tube assembly includes a first infusion tube and a second infusion tube. The first infusion tube is provided with a first valve, and the second infusion tube is provided with a second valve. One end of the first infusion tube and one end of the second infusion tube are both used to connect to the infusion tube. The other end of the first infusion tube is connected to one end of the storage tube, and the other end of the second infusion tube is connected to the other end of the storage tube.

[0010] Preferably, the inoculum outlet assembly includes a first inoculum outlet tube, a second inoculum outlet tube, and a third inoculum outlet tube; or, the inoculum outlet assembly includes a first inoculum outlet tube, a second inoculum outlet tube, a third inoculum outlet tube, and at least one spare inoculum outlet tube. The first inoculum outlet tube is equipped with a third valve, the second inoculum outlet tube is equipped with a fourth valve, the third inoculum outlet tube is equipped with a fifth valve, and the spare inoculum outlet tube is equipped with a sixth valve. Both the fifth and sixth valves are manual / automatic ball valves. One end of the first inoculum outlet tube is connected to one end of the storage tube, one end of the second inoculum outlet tube is connected to the other end of the storage tube, the other ends of both the first and second inoculum outlet tubes are connected to one end of the third inoculum outlet tube, and the other ends of both the first and second inoculum outlet tubes are connected to one end of the spare inoculum outlet tube. The other end of the third inoculum outlet tube and the other ends of each spare inoculum outlet tube are connected to different bag surface inoculation structures.

[0011] Preferably, it further includes at least one driving structure for driving the movement of the actuating element inside the liquid storage tube. The driving structure operates in an intermittent and / or alternating manner. The driving structure includes a servo motor, a transmission screw, and a liquid control board. The output shaft of the servo motor is connected to the transmission screw. The transmission screw is arranged parallel to the liquid storage tube. The liquid control board is sleeved on the outer periphery of the transmission screw and threadedly connected to the transmission screw. The liquid control board is magnetically coupled to the actuating element.

[0012] Preferably, the inoculation structure is further provided with a support, the support including a first fixing plate, a second fixing plate and a plurality of fixing rods, the first fixing plate and the second fixing plate being arranged opposite to each other, the fixing rods being able to pass through the first fixing plate, the liquid control plate and the second fixing plate and being fixedly connected to the first fixing plate and the second fixing plate respectively, the liquid storage tube being fixedly connected to the first fixing plate and the second fixing plate respectively, the liquid control plate being located between the first fixing plate and the second fixing plate, the liquid control plate being slidably connected to the fixing rods and the liquid storage tube respectively, and the transmission screw being rotatably connected to the first fixing plate and the second fixing plate respectively.

[0013] Preferably, the inoculator includes a bacterial delivery structure and an inoculation structure. The inlet of the bacterial delivery structure is connected to the outlet of the bacterial outlet tube assembly. The bacterial delivery structure is used to deliver liquid inoculum to the inoculation structure, and the inoculation structure is used to inoculate the substrate inside and outside the center of the substrate bag.

[0014] Preferably, the inoculator further includes a material cylinder, which is a hollow cylinder with openings at both ends. The material cylinder is located around the bacterial transport structure and the inoculation structure, and there are gaps between the material cylinder and the bacterial transport structure and the inoculation structure, respectively.

[0015] Preferably, multiple control units are arranged around the outer periphery of the material cylinder. Each control unit includes a trajectory control valve and a bag surface inoculum outlet pipe. The bag surface inoculum outlet pipe is installed on the outer wall of the material cylinder. The first end of the bag surface inoculum outlet pipe is connected to the sterilization port of the inoculum outlet pipe assembly. The trajectory control valve is located near the first end of the bag surface inoculum outlet pipe and is used to control whether the inoculum outlet pipe assembly delivers liquid inoculum into the bag surface inoculum outlet pipe. The second end of the bag surface inoculum outlet pipe extends to the outlet of the material cylinder and is used to inoculate the inner and outer layers of the substrate material on the bag surface of the inoculum bag. The trajectory control valve is a trajectory control solenoid valve, a trajectory control semi-automatic valve, or a trajectory control manual valve.

[0016] Preferably, the control unit further includes a shut-off valve, which is used to limit the pressure value at various points of the intermittent inoculation trajectory control device for controllable changes in the amount of liquid bacteria.

[0017] Preferably, the inoculation structure includes an inoculation tube and a storage tank, one end of the inoculation tube is connected to the storage tank, and the bottom of the storage tank is connected to the inoculation supply tube.

[0018] The present invention achieves the following technical effects compared to the prior art: The present invention provides a controllable, intermittent inoculation trajectory control device for liquid inoculum. The inoculum outlet tube assembly is connected to both ends of the inoculator, the bag surface inoculation structure, and the storage tube. Liquid inoculum is delivered to the inoculator and the bag surface inoculation structure via the outlet tube assembly. The bag surface inoculation structure includes multiple parallel control units. These control units can be individually opened and closed according to inoculation parameters and inoculation trajectory settings to control the inoculation trajectory pattern of the inner and outer layers of the substrate bag inside the substrate bag. The pressure in the storage tube is intermittent and adjustable according to a set interval value. Therefore, inoculation within the substrate bag can be achieved without a drive structure; that is, liquid inoculum is injected into the storage tube, and then pressure is applied to the storage tube. When intermittent injection of liquid inoculum into the substrate bag is required, only the opening and closing frequency of each control unit needs to be controlled. During continuous inoculation into the substrate bag, the device maintains... The pressure balance within the liquid storage tube is sufficient to simplify the operation. Simultaneously, by coordinating the intermittent operation of multiple control units (i.e., control units are active or inactive), or the alternating left and right injection structures (i.e., two sets of injection structures operating alternately), or the direct-spray intermittent injection structure (i.e., one set of injection structures with six rows of pipes operating directly and intermittently), the continuous, uninterrupted liquid inoculation trajectory within the mushroom bag of existing bulk inoculation devices is directly altered. This creates a novel, controllable, intermittent, and unique inoculation trajectory of liquid spawn within the edible mushroom bag. While ensuring the existing mycelial germination and colonization effects, this effectively avoids excessive inoculation, reduces input costs, and significantly increases the number of robust mycelial colonies within the mushroom bag (more germination points and more weak mycelia). Attached Figure Description

[0019] To more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the drawings used in the embodiments will be briefly introduced below. Obviously, the drawings described below are only some embodiments of the present invention. For those skilled in the art, other drawings can be obtained based on these drawings without creative effort.

[0020] Figure 1 This is a schematic diagram of the intermittent inoculation trajectory control device for controllable quantitative change of liquid bacterial strain in Example 1 at one angle (the bag surface inoculation structure is omitted). Figure 2This is a schematic diagram of the intermittent inoculation trajectory control device for controllable quantitative change of liquid bacterial strain in Example 1 from another angle (the bag surface inoculation structure is omitted). Figure 3 for Figure 1 Top view; Figure 4 for Figure 1 The right view; Figure 5 This is a schematic diagram of the bag surface inoculation structure in Example 1; Figure 6 for Figure 5 The main view; Figure 7 for Figure 6 AA section view; Figure 8 for Figure 5 Side view; Figure 9 To establish a continuous inoculation trajectory for shiitake mushroom spawn using existing bulk spawn production technology. Figure 1 ; Figure 10 To generate a parallel intermittent trajectory diagram for shiitake mushroom logs using the intermittent inoculation trajectory control device with controllable quantitative change of liquid inoculum in Example 1. Figure 11 To establish a continuous inoculation trajectory for black fungus spawn bags using existing bulk spawn production technology. Figure 1 ; Figure 12 To generate a parallel discontinuous trajectory diagram for the black fungus spawn bags using the intermittent inoculation trajectory control device with controllable quantitative change of liquid spawn in Example 1. Figure 13 To establish a continuous inoculation trajectory for shiitake mushroom spawn using existing bulk spawn production technology. Figure 2 ; Figure 14 To create a triangular intermittent trajectory diagram for shiitake mushroom logs using the intermittent inoculation trajectory control device with controllable quantitative change of liquid inoculum as described in Example 1. Figure 15 To establish a continuous inoculation trajectory for black fungus spawn bags using existing bulk spawn production technology. Figure 2 ; Figure 16 The discontinuous inoculation trajectory control device with controllable quantitative change of liquid strain in Example 1 is used to generate a triangular discontinuous trajectory diagram for the black fungus spawn bag. In the diagram: 1-Controllable intermittent inoculation trajectory control device for liquid inoculum, 2-Inoculation structure, 3-Bag surface inoculation structure, 4-Inoculum supply tube, 5-Storage tube, 6-First inoculum delivery tube, 7-Second inoculum delivery tube, 8-First valve, 9-Second valve, 10-First outlet tube, 11-Second outlet tube, 12-Third outlet tube, 13-Spare outlet tube, 14-Third valve, 15-Fourth valve, 16-Fifth valve, 17-Sixth valve, 18-First pneumatic valve body. 9-First valve body actuator, 20-Second pneumatic valve body, 21-Second valve body actuator, 22-Servo motor, 23-Transmission screw, 24-Liquid control board, 25-First fixed plate, 26-Second fixed plate, 27-Fixing rod, 28-Inoculum conveying shaft, 29-Material cylinder, 30-Spiral blade, 31-Control unit, 32-Bag surface inlet tube, 33-Trajectory control valve, 34-Flange, 35-Inoculum conveying channel, 36-Storage tank, 37-Inlet tube, 38-Main valve. Detailed Implementation

[0021] The technical solutions of the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are only some embodiments of the present invention, and not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those skilled in the art without creative effort are within the scope of protection of the present invention.

[0022] The purpose of this invention is to provide a controllable, intermittent inoculation trajectory control device for liquid microbial culture, in order to solve the problems existing in the prior art, so that the inoculation amount and inoculation trajectory of the liquid microbial culture can be controlled, avoiding excessive inoculation amount, and effectively reducing the overall input cost of the microbial culture bags without affecting the original inoculation effect.

[0023] To make the above-mentioned objects, features and advantages of the present invention more apparent and understandable, the present invention will be further described in detail below with reference to the accompanying drawings and specific embodiments.

[0024] Example 1 like Figures 1-8As shown, this embodiment provides a discontinuous inoculation trajectory control device 1 for controllable quantitative change of liquid microorganisms, including an inoculation structure 2, a bag surface inoculation structure 3, and at least one injection structure. When there is more than one injection structure, multiple injection structures are arranged in parallel. The inlet of the inoculation structure 2 is used for the introduction of liquid microorganisms, and the outlet of the inoculation structure 2 can be connected to each injection structure respectively. The injection structure includes a supply pipe 4, a storage pipe 5, a delivery pipe assembly, and an outlet pipe assembly. The supply pipe 4 is used for the introduction of liquid microorganisms, and a main valve 38 is provided on the supply pipe 4. The delivery pipe assembly is connected to the supply pipe 4 and the main valve 38 respectively. The two ends of the storage tube 5 are connected, and the inoculation tube assembly is connected to the inoculator, the bag surface inoculation structure 3, and the two ends of the storage tube 5, respectively. The pressure of the storage tube 5 is intermittent and can be adjusted according to the interval setting value. Thus, inoculation in the culture bag can be achieved without setting a drive structure. That is, liquid inoculum is injected into the storage tube 5, and then the storage tube 5 is pressurized. When intermittent injection of liquid inoculum into the culture bag is required, it is only necessary to control the opening and closing frequency of each control unit 31. When continuously inoculating into the culture bag, it is only necessary to maintain the pressure balance in the storage tube 5, making the operation simpler. When there is only one inoculation structure, the six rows of tubes in the inoculation structure can operate in a direct-injection intermittent manner. When there is more than one inoculation structure, the inoculation structures can be operated by alternating left and right sides. This ensures that the pressure value in the liquid storage tube 5 remains relatively stable during continuous high-speed inoculation. Liquid inoculum is delivered to the inoculator and the bag surface inoculation structure 3 through the inoculation tube assembly. The bag surface inoculation structure 3 includes multiple control units 31 arranged in parallel. These multiple control units 31 can be individually opened and closed according to the inoculation parameters (such as inoculation pressure, inoculation volume, and inoculation time) and the inoculation trajectory pattern, and are used for control. The morphology of the inoculation trajectory on the inner and outer layers of the substrate bag inside the spawn bag is directly altered by the intermittent operation of multiple control units 31. This changes the continuous and uninterrupted liquid inoculation trajectory within the spawn bag, a novel and controllable quantitative change and inoculation trajectory variation of liquid spawn within the edible mushroom spawn bag. This effectively avoids excessive inoculation while ensuring the existing mycelial germination and colonization effect, reducing input costs, and significantly increasing the number of robust mycelial colonies within the spawn bag (more germination points and more weak mycelia).

[0025] The inoculation tube assembly includes a first inoculation tube 6 and a second inoculation tube 7. A first valve 8 is provided on the first inoculation tube 6, and a second valve 9 is provided on the second inoculation tube 7. One end of both the first and second inoculation tubes is connected to the inoculation tube 4. The other end of the first inoculation tube 6 is connected to one end of the storage tube 5, and the other end of the second inoculation tube 7 is connected to the other end of the storage tube 5. In this embodiment, while the liquid bacteria in the cavity on one side of the actuating element in the storage tube 5 are discharged into the inoculator for inoculation, the liquid bacteria in the storage chamber 36 enter the cavity on the other side of the actuating element in the storage tube 5, thereby replenishing the liquid bacteria.

[0026] The colony discharge tube assembly includes a first colony discharge tube 10, a second colony discharge tube 11, and a third colony discharge tube 12, suitable for a single intermittent operation mode. Alternatively, the colony discharge tube assembly includes a first colony discharge tube 10, a second colony discharge tube 11, a third colony discharge tube 12, and at least one spare colony discharge tube 13. A third valve 14 is provided on the first colony discharge tube 10, a fourth valve 15 is provided on the second colony discharge tube 11, a fifth valve 16 is provided on the third colony discharge tube 12, and a sixth valve 17 is provided on the spare colony discharge tube 13. The fifth valve 16 and the sixth valve 17... All valves are manual / automatic integrated ball valves. One end of the first inoculum outlet tube 10 is connected to one end of the liquid storage tube 5, one end of the second inoculum outlet tube 11 is connected to the other end of the liquid storage tube 5, and the other ends of both the first and second inoculum outlet tubes 10 and 11 are connected to one end of the third inoculum outlet tube 12. The other ends of both the first and second inoculum outlet tubes 10 and 11 are connected to one end of the spare inoculum outlet tube 13. The other ends of the third inoculum outlet tube 12 and each spare inoculum outlet tube 13 are connected to different bag surface inoculation structures 3. In this embodiment, when a certain inoculum inoculation structure malfunctions, the spare inoculum outlet tube 13 can connect the normal inoculum inoculation structure to the inoculator, ensuring the inoculation efficiency of the inoculator and preventing the inoculation work of the inoculator and the inoculum bag surface from being affected by the malfunction of one inoculum inoculation structure.

[0027] In this embodiment, at least one driving structure is also included. The driving structure is used to drive the movement of the actuating element inside the liquid storage tube 5. The driving structure operates in an intermittent and / or alternating manner. By changing the inoculation propulsion state of the driving structure from the original one-spray-to-the-end working stroke to a controllable intermittent propulsion inoculation working state, and in conjunction with the intermittent working state of multiple control units 31, the trajectory of the continuous and uninterrupted liquid inoculation in the inoculation bag, which is a hallmark of existing bulk inoculation devices, can be directly changed. The driving structure includes a servo motor 22, a transmission screw 23, and a liquid control plate 24. The output shaft of the servo motor 22 is connected to the transmission screw 23. The transmission screw 23 is arranged parallel to the liquid storage tube 5. The liquid control plate 24 is sleeved on the outer periphery of the transmission screw 23 and threadedly connected to the transmission screw 23. The liquid control plate 24 is magnetically coupled to the actuating element. As the liquid control plate 24 moves outside the liquid storage tube 5, the actuating element moves inside the liquid storage tube 5, thereby acting on the liquid bacteria inside the liquid storage tube 5. Meanwhile, due to the magnetic coupling between the liquid control plate 24 and the actuating elements in each liquid storage tube 5, multiple actuating elements can be linked together, enabling each injection structure to work simultaneously.

[0028] In this embodiment, when the trajectory of the liquid bacteria needs to be arranged in a triangular equidistant pattern, two driving structures can be used to drive two sets of inoculation structures to output the liquid bacteria, and the two driving structures can work alternately. At this time, the trajectory of the liquid bacteria can be arranged in a triangular equidistant pattern.

[0029] In this embodiment, when there is only one inoculation structure, a single inoculation structure can be used to output liquid bacteria in a single intermittent manner. At this time, it is no longer necessary for two driving structures to work alternately, thereby forming a discrete point / segment distribution of discontinuous inoculation trajectory.

[0030] In this embodiment, a first pneumatic valve body 18 is provided at one end of the liquid storage pipe 5, and a first valve body actuator 19 is provided at the first pneumatic valve body 18. A second pneumatic valve body 20 is provided at the other end of the liquid storage pipe 5, and a second valve body actuator 21 is provided at the second pneumatic valve body 20. Both the first valve body actuator 19 and the second valve body actuator 21 are solenoid valves.

[0031] The inoculation structure also includes a support frame, comprising a first fixing plate 25, a second fixing plate 26, and several fixing rods 27. The first fixing plate 25 and the second fixing plate 26 are arranged opposite to each other. The fixing rods 27 can pass through the first fixing plate 25, the liquid control plate 24, and the second fixing plate 26, and are fixedly connected to the first fixing plate 25 and the second fixing plate 26 respectively. The liquid storage tube 5 is fixedly connected to the first fixing plate 25 and the second fixing plate 26 respectively. The liquid control plate 24 is located between the first fixing plate 25 and the second fixing plate 26, and is slidably connected to the fixing rods 27 and the liquid storage tube 5 respectively. The transmission screw 23 is rotatably connected to the first fixing plate 25 and the second fixing plate 26 respectively. The fixing rods 27 and the liquid storage tube 5 can simultaneously serve as guides when the liquid control plate 24 slides. The transmission screw 23 is rotatably connected to the first fixing plate 25 and the second fixing plate 26 respectively via bearings.

[0032] The inoculator includes a spore delivery structure and an inoculation structure. The inlet of the spore delivery structure is connected to the outlet of the spore outlet tube assembly. The spore delivery structure is used to transport liquid inoculum to the inoculation structure, and the inoculation structure is used to inoculate the substrate inside and outside the center of the substrate bag. In this embodiment, the spore delivery structure and the inoculation structure are consistent with the prior art. A spiral blade 30 is provided on the outside of the spore delivery shaft 28 of the spore delivery structure, which can be used to transport the substrate during the inoculation process. A spore delivery channel 35 is provided inside the spore delivery shaft 28. In addition, in this embodiment, the inoculation structure is a multi-dimensional inoculation structure. Those skilled in the art can also make adaptive adjustments to its structure according to actual needs.

[0033] The inoculator also includes a material cylinder 29, which is a hollow cylinder with openings at both ends. The material cylinder 29 is located around the inoculation and feeding structures, with gaps between it and both structures. A flange 34 is provided at one end of the material cylinder 29, allowing it to be fixed to the outlet of the feed hopper. Spiral blades 30 on the feeding shaft 28 are located within the feed hopper and the material cylinder 29. As the feeding shaft 28 rotates, the sterilized and cooled substrate in the hopper is conveyed to the material cylinder 29 by the spiral blades 30, and continuously compressed forward into the plastic bag through the opening at the front end of the material cylinder 29. Simultaneously, multiple control units 31 located on the outer periphery of the material cylinder 29 can discharge materials according to the required trajectory program.

[0034] Multiple control units 31 are arranged around the outer periphery of the material cylinder 29. Each control unit 31 includes a trajectory control valve 33 and a bag surface inoculum outlet pipe 32. The bag surface inoculum outlet pipe 32 is installed on the outer wall of the material cylinder 29. The first end of the bag surface inoculum outlet pipe 32 is connected to the sterilization port of the inoculum outlet pipe assembly. The trajectory control valve 33 is located near the first end of the bag surface inoculum outlet pipe 32 and is used to control whether the inoculum outlet pipe assembly delivers liquid inoculum into the bag surface inoculum outlet pipe 32. The second end of the bag surface inoculum outlet pipe 32 extends to the outlet of the material cylinder 29 and is used to inoculate the inner and outer layers of the substrate on the surface of the inoculum bag. By controlling the intermittent opening of each trajectory control valve 33, controllable quantitative intermittent inoculation is achieved. In this embodiment, the trajectory control valve 33 is a trajectory control solenoid valve, a trajectory control semi-automatic valve, or a trajectory control manual valve. The inoculation structure 2 includes an inoculation tube 37 and a storage tank 36. One end of the inoculation tube 37 is connected to the storage tank 36, and the bottom of the storage tank 36 is connected to the supply tube 4. When the main valve 38 is opened, the liquid bacteria in the storage tank 36 can fall into the supply tube 4 under the action of gravity.

[0035] In this embodiment, the control unit 31 also includes a shut-off valve, which is used to limit the pressure value at various points of the intermittent inoculation trajectory control device with controllable changes in the amount of liquid bacteria, thereby protecting the entire device when no drive structure is provided. In this embodiment, the shut-off valve is preferably a high-pressure pneumatic shut-off valve, and the trajectory control valve 33 is preferably a high-frequency solenoid valve.

[0036] This embodiment, while maintaining the basic structure of the existing bulk material bagging machine inoculation device, adds a novel program control scheme to the bagging machine's spawn dispensing cylinder electrically controlled propulsion spawn injection system. This changes the servo motor 22's spawn injection propulsion state from a single, continuous spray to a controllable, intermittent propulsion spawn injection state. This directly alters the continuous, uninterrupted liquid inoculation trajectory within the spawn bag, as characteristic of existing bulk material inoculation devices, creating a novel, controllable, intermittent, and unique inoculation trajectory of liquid spawn within the edible mushroom spawn bag. Furthermore, it solves the following problems: (1) It solves the problem of excessive inoculation amount in the existing bulk material spawn making process. For example, the inoculation amount of conventional shiitake mushroom spawn (empty bag size 60mm×18mm, full bag size 45mm×12mm) in the existing bulk material process is an average of 220ml / spawn. However, the latest solution reduces the inoculation amount by at least 50% and the amount of inoculated liquid will be as low as 110ml / spawn due to the use of intermittent inoculation control program. (2) Since the solution in this embodiment can save at least 50% of the liquid inoculum volume in the spawn bag compared to the existing bulk material spawn making process, the cost of producing liquid spawn in the latest inoculation control solution is reduced by at least 50%; (3) The inoculation amount and inoculation trajectory can be controlled at will. In this embodiment, the solution can be changed by altering the propulsion program and working state of the servo motor 22 and setting the travel distance of the bacterial liquid tank according to process requirements, thereby determining the spacing and amount of liquid bacteria in the intermittent inoculation lines in the bacterial bag. The longer the spacing between the inoculation lines, the less the inoculation amount. The inoculation amount is the least when the same inoculation lines are in a triangular intersection trajectory. (4) The amount of liquid inoculation is reduced without reducing the efficiency. The existing bulk material tumbler process requires too much liquid inoculation (the traditional liquid single-sided inoculation amount for large mushroom tumblers is generally around 60ml). The existing bulk material process has an average inoculation amount of 220ml / tumbler, while the liquid inoculation amount of the bulk material tumbler process in this embodiment is only 110ml / tumbler on average, which is as close as possible to the inoculation amount of the traditional single-sided inoculation. However, it still maintains the special technological advantage of the bulk material process of fast mycelial growth. It can ensure that the germination speed before the reduction of the amount of mycelium remains unchanged even when the inoculation amount is reduced by 50%. (5) Since the liquid inoculation control scheme in this embodiment can reduce the inoculation amount by at least 50%, the volume of the terminal culture tank used for liquid culture is reduced by at least 50%, and the cost of the terminal culture inoculation tank is reduced by at least 60%, thereby directly saving the significant investment cost of the terminal culture tank. (6) This embodiment can achieve multiple inoculation trajectories, including but not limited to parallel discontinuous, triangular equidistant arrangement and various asymmetric liquid bacterial inoculation trajectories; (7) This embodiment can substantially improve the germination quality of the mushroom bag, making the surface environment of the mushroom bag more suitable for the growth conditions of edible fungi. One of the characteristics of bulk inoculation is that while inoculating the liquid spawn inside, the spawn is also inoculated into various dimensions of the substrate of the mushroom bag. Since the liquid spawn is a liquid culture medium containing sugar with a comprehensive ratio of chemical and grain, the large amount of liquid spawn inoculated into the surface of the culture medium inside the mushroom bag will directly lead to an increase in monosaccharides on the surface of the substrate, making the surface environment of the substrate culture more acidic, creating the best infection environment for various miscellaneous fungi. At the same time, the liquid spawn has the ability to germinate mycelial balls and mycelial fragments in the primary mycelial state after colonization and germination. The initial mycelium needs 24 hours to adapt after germination before it gradually becomes robust. Therefore, the more liquid inoculation points on the substrate surface, the more weak mycelial groups there are, and the worse the mycelial quality. The bag surface is also the initial point for contamination by miscellaneous bacteria. At the same time, the amount of inoculum introduced by the liquid solution is reduced by several times, which directly reduces the amount of liquid inoculum injected into the outer layer of the substrate inside the bag. This creates more blank space for the mycelium to grow and rejuvenate. While reducing the increase of monosaccharides in the substrate on the bag surface, it effectively increases the robustness of the mycelium and the quality of the microenvironment on the bag surface, making the overall process more perfect and more in line with the growth law of fungi and the process management requirements of mycelial bag cultivation and fruiting period.

[0037] Application Example 1 like Figures 9-10 As shown, this application embodiment uses the intermittent inoculation trajectory control device 1 with controllable quantitative change of liquid bacterial strain in embodiment 1 to perform liquid bacterial strain inoculation, and the inoculation trajectory is parallel intermittent.

[0038] Application Example 2 like Figures 11-12 As shown, this application embodiment uses the intermittent inoculation trajectory control device 1 with controllable quantitative change of liquid spawn from Embodiment 1 to inoculate black fungus spawn bags with liquid spawn, and the inoculation trajectory is parallel intermittent.

[0039] Application Example 3 like Figures 13-14 As shown, this application embodiment uses the intermittent inoculation trajectory control device 1 with controllable quantitative change of liquid spawn from Embodiment 1 to inoculate shiitake mushroom logs with liquid spawn. The inoculation trajectory is a triangular intermittent pattern.

[0040] Application Example 4 like Figures 15-16 As shown, this application embodiment uses the intermittent inoculation trajectory control device 1 with controllable quantitative change of liquid spawn from Embodiment 1 to inoculate black fungus spawn bags with liquid spawn. The inoculation trajectory is a triangular intermittent pattern.

[0041] Specific examples have been used to illustrate the principles and implementation methods of this invention. The descriptions of the above embodiments are only for the purpose of helping to understand the method and core ideas of this invention. Furthermore, those skilled in the art will recognize that, based on the ideas of this invention, there will be changes in the specific implementation methods and application scope. Therefore, the content of this specification should not be construed as a limitation of this invention.

Claims

1. A device for controlling the intermittent inoculation trajectory of liquid bacterial strains with controllable quantitative changes, characterized in that: The device includes an inoculation structure (2), a bag surface inoculation structure (3), and at least one injection structure. The inlet of the inoculation structure (2) is used for the introduction of liquid inoculum, and the outlet of the inoculation structure (2) is connected to each of the injection structures. The injection structure includes a supply tube (4), a storage tube (5), a delivery tube assembly, and an outlet tube assembly. The supply tube (4) is used for the introduction of liquid inoculum. The delivery tube assembly is connected to both ends of the supply tube (4) and the storage tube (5). The outlet tube assembly is connected to the inoculator and the bag surface inoculation structure (3). The liquid storage tube (5) is connected to both ends. The pressure of the liquid storage tube (5) can be adjusted according to the spacing setting value. When there is more than one inoculation structure, the inoculation structure is operated in a left-right interchangeable manner. Liquid bacteria are delivered to the inoculator and the bag surface inoculation structure (3) through the inoculation tube assembly. The bag surface inoculation structure (3) includes multiple control units (31) arranged in parallel. The multiple control units (31) can be set to open and close individually according to the inoculation parameter setting and the inoculation trajectory shape, and are used to control the shape of the inoculation trajectory of the inner and outer layers of the substrate bag inside the mushroom bag.

2. The intermittent inoculation trajectory control device for controllable quantitative change of liquid bacterial strains according to claim 1, characterized in that: The infusion tube assembly includes a first infusion tube (6) and a second infusion tube (7). A first valve (8) is provided on the first infusion tube (6), and a second valve (9) is provided on the second infusion tube (7). One end of the first infusion tube (6) and one end of the second infusion tube (7) are both used to connect to the infusion tube (4). The other end of the first infusion tube (6) is connected to one end of the storage tube (5), and the other end of the second infusion tube (7) is connected to the other end of the storage tube (5).

3. The intermittent inoculation trajectory control device for controllable quantitative change of liquid bacterial strains according to claim 1, characterized in that: The colony discharge tube assembly includes a first colony discharge tube (10), a second colony discharge tube (11), and a third colony discharge tube (12), or the colony discharge tube assembly includes a first colony discharge tube, a second colony discharge tube, a third colony discharge tube, and at least one spare colony discharge tube. A third valve (14) is provided on the first colony discharge tube (10), a fourth valve (15) is provided on the second colony discharge tube (11), a fifth valve (16) is provided on the third colony discharge tube (12), and a sixth valve (17) is provided on the spare colony discharge tube (13). Both the fifth valve (16) and the sixth valve (17) are manual / automatic ball valves. One end of the first inoculum outlet tube (10) is used to connect to one end of the storage tube (5), one end of the second inoculum outlet tube (11) is connected to the other end of the storage tube (5), the other ends of the first inoculum outlet tube (10) and the second inoculum outlet tube (11) are both used to connect to one end of the third inoculum outlet tube (12), the other ends of the first inoculum outlet tube (10) and the second inoculum outlet tube (11) are both used to connect to one end of the spare inoculum outlet tube (13), and the other end of the third inoculum outlet tube (12) and the other end of each of the spare inoculum outlet tubes (13) are both used to connect to different bag surface inoculation structures (3).

4. The intermittent inoculation trajectory control device for controllable quantitative change of liquid bacterial strains according to claim 1, characterized in that: It also includes at least one drive structure, which is used to drive the movement of the actuating element in the liquid storage tube (5), and the drive structure is in an intermittent working state and / or an alternating working state. The drive structure includes a servo motor (22), a transmission screw (23) and a liquid control plate (24). The output shaft of the servo motor (22) is connected to the transmission screw (23). The transmission screw (23) is arranged parallel to the liquid storage tube (5). The liquid control plate (24) is sleeved on the outer periphery of the transmission screw (23) and threadedly connected to the transmission screw (23). The liquid control plate (24) is magnetically coupled to the actuating element.

5. The intermittent inoculation trajectory control device for controllable quantitative change of liquid bacterial strains according to claim 4, characterized in that: The inoculation structure is also provided with a support, which includes a first fixing plate (25), a second fixing plate (26) and a plurality of fixing rods (27). The first fixing plate (25) and the second fixing plate (26) are arranged opposite to each other. The fixing rods (27) can pass through the first fixing plate (25), the liquid control plate (24) and the second fixing plate (26) and are fixedly connected to the first fixing plate (25) and the second fixing plate (26) respectively. The liquid storage tube (5) is fixedly connected to the first fixing plate (25) and the second fixing plate (26) respectively. The liquid control plate (24) is located between the first fixing plate (25) and the second fixing plate (26). The liquid control plate (24) is slidably connected to the fixing rods (27) and the liquid storage tube (5) respectively. The transmission screw (23) is rotatably connected to the first fixing plate (25) and the second fixing plate (26) respectively.

6. The intermittent inoculation trajectory control device for controllable quantitative change of liquid bacterial strains according to claim 1, characterized in that: The inoculator includes a bacterial delivery structure and an inoculation structure. The inlet of the bacterial delivery structure is connected to the outlet of the bacterial outlet tube assembly. The bacterial delivery structure is used to deliver liquid inoculum to the inoculation structure. The inoculation structure is used to inoculate the substrate inside and outside the substrate of the inoculum bag. The inoculation structure is a multi-dimensional inoculation structure.

7. The intermittent inoculation trajectory control device for controllable quantitative change of liquid bacterial strains according to claim 6, characterized in that: The inoculator also includes a material cylinder (29), which is a hollow cylinder with openings at both ends. The material cylinder (29) is located outside the inoculation structure and the inoculation structure, and there are gaps between the material cylinder (29) and the inoculation structure and the inoculation structure respectively.

8. The intermittent inoculation trajectory control device for controllable quantitative change of liquid bacterial strains according to claim 7, characterized in that: Multiple control units (31) are arranged around the outer periphery of the material cylinder (29). Each control unit (31) includes a trajectory control valve (33) and a bag surface inoculum outlet tube (32). The bag surface inoculum outlet tube (32) is installed on the outer wall of the material cylinder (29). The first end of the bag surface inoculum outlet tube (32) is connected to the sterilization port of the inoculum outlet tube assembly. The trajectory control valve (33) is located near the first end of the bag surface inoculum outlet tube (32) and is used to control whether the inoculum outlet tube assembly delivers liquid inoculum into the bag surface inoculum outlet tube (32). The second end of the bag surface inoculum outlet tube (32) extends to the outlet of the material cylinder (29) and is used to inoculate the inner and outer layers of the bag surface substrate of the inoculum bag. The trajectory control valve (33) is a trajectory control solenoid valve, a trajectory control semi-automatic valve, or a trajectory control manual valve.

9. The intermittent inoculation trajectory control device for controllable quantitative change of liquid bacterial strains according to claim 8, characterized in that: The control unit (31) also includes a shut-off valve, which is used to limit the pressure value at various points of the intermittent inoculation trajectory control device for controllable changes in the amount of liquid bacteria.

10. The intermittent inoculation trajectory control device for controllable quantitative change of liquid bacterial strains according to claim 1, characterized in that: The inoculation structure (2) includes an inoculation tube (37) and a storage tank (36). One end of the inoculation tube (37) is connected to the storage tank (36), and the bottom of the storage tank (36) is connected to the inoculation tube (4).

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