Compound composition containing rotenone and metarhizium anisopliae and application thereof

Abstract

The invention relates to a compound composition containing metarhizium anisopliae and rotenone and application of the compound composition, and belongs to the technical field of biopesticides. The effective components of the compound composition are rotenone and metarhizium anisopliae MaGX19S02, and the compounding mass ratio of the metarhizium anisopliae MaGX19S02 to the rotenone is (0.1-9): (0.1-1). According to the invention, by compounding the active components of metarhizium anisopliae and rotenone, the defects that a single component rotenone is high in price, and metarhizium anisopliae is poor in quick-acting property, unstable in pesticide effect and the like are effectively overcome; the compound composition containing the metarhizium anisopliae and the rotenone is an environment-friendly pesticide composition, and the active ingredients of the metarhizium anisopliae and the rotenone are in a reasonable compounding proportion range and have a synergistic effect on prevention and control of tetranychus cinnabarinus.

Description

Technical Field

[0001] This invention relates to a compound composition containing rotenone and Metarhizium anisopliae and its application, belonging to the field of biopesticide technology. Background Technology

[0002] Insecticides play a crucial role in pest control. With increasing environmental and health awareness, low-toxicity, natural insecticides are gradually replacing chemical insecticides, and plant-derived and microbial insecticides are gaining popularity in the market. Rotenone is an important plant-derived active substance, mainly extracted from the roots and stems of plants in the genera *Rotenone*, *Buddleja*, and *Eriocaulon*, and is currently widely used in pest control. Rotenone possesses broad-spectrum insecticidal activity, such as effectively inhibiting insect respiration, and exhibiting strong antifeedant, contact, and stomach poison effects. Furthermore, rotenone degrades rapidly in air and light, resulting in low residue risk, and is therefore considered an environmentally friendly biological insecticide.

[0003] Metarhizium anisopliae, a type of beetle fungus, is an important entomopathogenic fungus with broad-spectrum insecticidal activity, capable of parasitizing over 1000 insect species. Taxonomically, it belongs to the kingdom Fungi, phylum Ascomycota, class Sordariomycetes, order Hypocreales, family Clavicipitaceae, and genus Metarhizium. Its conidia are often processed into wettable powders, water-dispersible granules, oil suspensions, and dispersible oil suspensions, which can be used to control various agricultural and forestry pests, such as fall armyworm, thrips, locusts, whiteflies, and aphids. Metarhizium anisopliae insecticides have advantages such as being natural, non-toxic, lacking resistance, easy to produce, relatively inexpensive, and environmentally friendly. However, its relatively poor speed of action and unstable efficacy limit its large-scale application.

[0004] If the advantages of both rotenone and Metarhizium anisopliae can be combined to compensate for their respective shortcomings, a compound agent with rapid, long-lasting insecticidal effects and a relatively low price can be developed, which will undoubtedly have a broad market prospect and generate significant economic benefits. However, research on the mixture of rotenone and entomopathogenic fungi is rarely reported. This invention aims to develop a compound insecticide of rotenone and Metarhizium anisopliae to promote the development of biopesticides and contribute to improving environmental quality and food safety. Summary of the Invention

[0005] In order to overcome the shortcomings of the prior art, the present invention aims to provide a compound composition containing Metarhizium anisopliae and rotenone and its application.

[0006] To achieve the above objectives, the technical solution adopted by the present invention is as follows: a compound composition containing Metarhizium anisopliae and rotenone, wherein the effective components of the compound composition are rotenone and Metarhizium anisopliae MaGX19S02, and the mass ratio of Metarhizium anisopliae MaGX19S02 to rotenone is 0.1-9:0.1-1.

[0007] As a preferred embodiment of the compound composition described in this invention, the mass ratio of Metarhizium anisopliae MaGX19S02 to rotenone is 9:0.1, 2.3:0.1, 1:0.1, 4:1, and 1:1.

[0008] As a preferred embodiment of the compound composition described in this invention, the mass ratio of Metarhizium anisopliae MaGX19S02 to rotenone is 4:1.

[0009] Another object of the present invention is to provide a dispersible oil suspension, wherein the effective components of the dispersible oil suspension include Metarhizium anisopliae MaGX19S02 and rotenone as described in any one of claims 1-3; the dispersible oil suspension further includes adjuvants, including emulsifiers, non-aqueous dispersants, aqueous dispersants, thickeners, ultraviolet protectants and oil bases.

[0010] As the dispersible oil suspension agent of the present invention, it comprises the following components by mass percentage: 0.1-9% Metarhizium anisopliae, 0.1-1% rotenone, 14-16% emulsifier, 2-3% non-aqueous dispersant, 2-3% aqueous dispersant, 4-5% thickener, 0.5-1% UV protectant, and the balance being oil base.

[0011] As the dispersible oil suspension agent of the present invention, the dispersible oil suspension agent comprises the following components by mass percentage: 4% Metarhizium anisopliae, 1% rotenone, 14% emulsifier, 3% non-aqueous dispersant, 3% aqueous dispersant, 4% thickener, 0.5% UV protectant, and 70.5% oil base.

[0012] As a dispersible oil suspension agent according to the present invention, the emulsifier includes TENSIOFIX 96DB10, Atlox-4914, or Atlas G-5002L; the non-aqueous dispersant includes Atlox-4914; the aqueous dispersant includes Atlas G-5002L or dispersant 5040; the thickener includes TENSIOFIX-869, Atlox Rheostrux-100, or A200; the UV protectant includes TiO2, ascorbic acid, ZnO, fluorescent whitening agent, or sodium carboxymethyl starch; and the oil base includes soybean oil.

[0013] Preferably, the dispersible oil suspension comprises, by weight percentage, the following components: 1% rotenone, 4% Metarhizium anisopliae, 14% TENSIOFIX 96DB10, 3% Atlox-4914, 3% Atlas G-5002L, 4% AtloxRheostrux-100, 0.5% ZnO, and 70.5% soybean oil.

[0014] Furthermore, this invention provides the application of the aforementioned compound composition or the aforementioned dispersible oil suspension in the prevention and / or control of agricultural pests. The agricultural pest is the carmine spider mite.

[0015] Compared with the prior art, the beneficial effects of the present invention are as follows: (1) The present invention provides a compound composition containing Metarhizium anisopliae and rotenone, which is an environmentally friendly pesticide composition. Its active ingredients Metarhizium anisopliae and rotenone have a synergistic effect on the control of Tetranychus cinnabarinus within a reasonable compound ratio range. (2) By combining the active ingredient Metarhizium anisopliae with rotenone, the drawbacks of the single ingredient rotenone, such as its high price, poor fast-acting properties, and unstable efficacy of Metarhizium anisopliae, are effectively solved. The present invention provides a compound composition containing Metarhizium anisopliae and rotenone that can be produced on a large scale in industrial production. Attached Figure Description

[0016] Figure 1 The effect of oil base on the spore germination rate of Metarhizium anisopliae.

[0017] Figure 2 Figure 1 shows the results of an indoor potted plant experiment on the effects of a 5% Metarhizium anisopliae·rotenone dispersible oil suspension diluted 100 times on the carmine spider mite; among which, Figure 2 The AB in the sample represents the state of the carmine spider mite after spraying with a 5% Metarhizium anisopliae·rotenone dispersible oil suspension diluted 100 times for 3 days; the mites were basically dead. Figure 2 CE in the figure represents the state of the worms after 5 days of moisturizing treatment; Figure 2 F in the figure represents the morphology of the worms inoculated into PDA plate cultures.

[0018] Figure 3 The study investigated the oviposition inhibition of different dosage formulations on Tetranychus cinnabarinus. Detailed Implementation

[0019] To better illustrate the purpose, technical solution, and advantages of this invention, the invention will be further described below with reference to specific drawings and embodiments. Unless otherwise specified, the experimental methods used in the embodiments are conventional methods, and the materials and reagents used are commercially available unless otherwise specified.

[0020] Example 1: Verification of the effects of rotenone, emulsifier, non-aqueous dispersant, aqueous dispersant, thickener, UV protectant, and oil base on bacterial activity.

[0021] 1. Activity of rotenone against entomopathogenic fungi

[0022] Spore suspensions of three strains, MaGX19S02, BbHW18, and BbGX6502, which exhibit high activity against Tetranychus cinnabarin, were spread on PDA plates containing rotenone. After 18 h, the spores were observed under a microscope, and the spore germination rate of each strain was calculated, as shown in Table 1.

[0023] Table 1. Effects of rotenone on spore germination rate of entomopathogenic fungi.

[0024] The data in Table 1 show the effects of rotenone at three concentrations (50 / 150 / 500 mg / L) on the spore germination of GX19S02, BbHW18, and BbGX6502. Compared with the control (CK), rotenone at all three concentrations had no significant effect on the mycelial growth and spore germination rate of Metarhizium anisopliae MaGX19S02 strain.

[0025] 2. Activity of different surfactants against Metarhizium anisopliae MaGX19S02

[0026] The safety screening results for single-dose agents are shown in Table 2:

[0027] Table 2. Spore germination rate of Metarhizium anisopliae MaGX19S02 in different surfactants.

[0028] Experimental Results: After 3 days of treatment with pure Metarhizium anisopliae spore powder, surfactant TD-4416 significantly reduced the spore germination rate compared to the control group. The spore germination rates of other surfactants showed no significant difference compared to the control group. After 20 days of treatment, the spore germination rates of most surfactants were significantly reduced compared to the control group, indicating that these surfactants inhibited the germination of Metarhizium anisopliae spores. However, the spore germination rates of Atlox-4914, dispersant 5040, Atlas G-5002L, Zephrym-PD2206, TENSIOFIX 96DB10, Atlas G-1096, Atlox-4916, and Atlas G-1086 showed no significant difference compared to the control group, indicating that these surfactants had good safety for Metarhizium anisopliae spores. Based on this, seven surfactants that showed a spore germination rate exceeding 55% after 20 days of treatment were selected for further experiments. These seven surfactants were Atlox-4914, dispersant 5040, Atlas G-5002L, Zephrym-PD2206, TENSIOFIX 96DB10, Atlox-4916, and Atlas G-1086.

[0029] 3. Safety of different thickeners against Metarhizium anisopliae MaGX19S02

[0030] Table 3. Spore germination rate of Metarhizium anisopliae MaGX19S02 in different thickeners.

[0031] Experimental results: Table 3 shows that after 10 days of storage at room temperature, the three thickeners TENSIOFIX-869, Atlox Rheostrux 100, and Evonik A200 showed the best compatibility with Metarhizium anisopliae spores and did not affect spore germination. There were no significant differences between the treatment groups.

[0032] 4. Safety of Metarhizium anisopliae MaGX19S02 against UV protectants under different conditions

[0033] Table 4. Germination rate of Metarhizium anisopliae MaGX19S02 under different conditions in UV protectant

[0034] The results, as shown in Table 4, indicate that the five UV protectants—TiO2, ascorbic acid, ZnO, fluorescent whitening agent, and sodium carboxymethyl starch—showed good compatibility with *Metarhizium anisopliae*. At 10 days, none of the different UV protectants significantly affected the spore germination rate of *Metarhizium anisopliae*. Under UV irradiation for 2 h and 6 h, the addition of different UV protectants did not significantly affect the spore germination rate of *Metarhizium anisopliae*, and the spore germination rate remained high even under short-term UV irradiation. ZnO exhibited a high germination rate under both room temperature storage and UV irradiation, and was therefore selected for subsequent experiments.

[0035] 5. Compatibility of Metarhizium anisopliae with oil-based materials

[0036] The results are as follows Figure 1 As shown, the spore germination rate of *Metarhizium anisopliae* pure spore powder stored in methyl oleate was extremely low, significantly lower than that of the control group and other oil-based treatments at both 3 and 5 days. *Metarhizium anisopliae* pure spore powder showed good preservation effects in vegetable oils, with the highest spore germination rate observed in soybean oil at 15 days. Considering the combined effects of rotenone and *Metarhizium anisopliae* pure spore powder in soybean oil, soybean oil was selected as the oil base.

[0037] Example 2: Virulence determination of rotenone and Metarhizium anisopliae strain MaGX19S02 against Tetranychus carmine

[0038] 1. Materials

[0039] The tested strain, Metarhizium anisopliae MaGX19S02, was isolated, purified, and preserved in our laboratory.

[0040] Test insect source: Tetranychus cinnabarinus ( Tetranychus cinnabarinus The seedlings were donated by Professor Huang Jiguang's research group from the Department of Pesticides, College of Plant Protection, South China Agricultural University, and were later used to feed the seedlings.

[0041] Test reagent: 98% rotenone, purchased from Baoji Fangcheng Development Co., Ltd.

[0042] 2. Test methods

[0043] This experiment used the Cotoxicity Factor (CF) to evaluate the combined effect of the two, as shown in the formula (CF≤-20: antagonistic effect; CF≥20: synergistic effect; -20≤CF≤20: additive effect).

[0044]

[0045] 3. Experimental Results and Analysis

[0046] Table 5. Activity of rotenone against Tetranychus carmine.

[0047] As shown in Table 5, the carmine spider mite becomes more sensitive to rotenone as the concentration of rotenone treatment increases. The corrected mortality rate of carmine spider mites under 72h and 500mg / L rotenone treatment is as high as 94.83%, indicating that rotenone has high activity against carmine spider mites.

[0048] Table 6. Activity of Metarhizium anisopliae MaGX19S02 against Tetranychus carmineus.

[0049] Table 6 shows the experimental results: the activity of Metarhizium anisopliae MaGX19S02 against Tetranychus carmine increased with increasing spore concentration, and the mortality rate also increased accordingly. At 1×10⁻⁶ spore concentrations... 8 The *Tetranychus cinnabarinus* strain showed the highest activity against *Metarhizium anisopliae* strain MaGX19S02 under treatment, with a corrected mortality rate of 93.75% after 7 days of treatment, indicating high activity. However, the corrected mortality rate was only 53.14% after 3 days of treatment and 60.16% after 5 days, indicating that the single *Metarhizium anisopliae* strain MaGX19S02 had poor rapid efficacy.

[0050] Table 7. Determination of the toxicity of rotenone and Metarhizium anisopliae strain MaGX19S02 against Tetranychus carmine.

[0051] Note: The data in the table are the toxicity test results of Rotenone spray treatment 3 days after treatment and Metarhizium anisopliae strain MaGX19S02 suspension spray treatment against Tetranychus cinnabarinus 7 days after treatment.

[0052] In summary, the concentration of the spore suspension of strain MaGX19S02 was set at 1×10⁻⁶. 8 5×10 7 2×10 7 1×10 7 8×10 6 The spore / mL concentration of rotenone was set at 500, 250, 125, 62.5, and 31.25 mg / L. The activity of rotenone against *Tetranychus carinata* under different treatment conditions is shown in Tables 5-6. The toxicity against *Tetranychus carinata* under different treatment conditions is shown in Table 7. Three days after treatment, the median lethal concentration (LD50) of rotenone against *Tetranychus carinata* was 188.02 mg / L. Seven days after treatment, the LD50 of strain MaGX19S02 against *Tetranychus carinata* was 2.73 × 10⁻⁶ mg / L. 7 Spores / mL.

[0053] Example 3: Virulence determination of rotenone and Metarhizium anisopliae strain MaGX19S02 against Tetranychus carmine var. cinnabarin at different ratios

[0054] The results obtained after treating *Tetranychus cinnabarinus* with the compound agent for 1, 3, and 5 days are shown in Table 8. When the mass ratio (w / w) of *Metarhizium anisopliae* MaGX19S02 to rotenone was 9:0.1, 2.3:0.1, 1:0.1, 4:1, and 1:1, the CF value was >20 at 3 days, and the activity against *Tetranychus cinnabarinus* met the synergistic requirements of the compound agent.

[0055] Table 8. Virulence determination of different compound ratios of rotenone and Metarhizium anisopliae strain MaGX19S02 against Tetranychus carmine.

[0056] Note: Spray assay, 20 female adult mites per treatment. The data in the table are the mortality rate and synergistic effect of different ratios of Metarhizium anisopliae MaGX19S02 and rotenone on Tetranychus carmine after 1 day, 3 days and 5 days. The mortality rate is corrected to the mean of 3 replicates ± standard error.

[0057] Experimental results: The results obtained after treating Tetranychus cinnabarinus with the compound agent for 1 day, 3 days and 5 days are shown in Table 8. When the ratio of MaGX19S02 to rotenone is 4:1, the CF value is >20 at 3 days, and the activity against Tetranychus cinnabarinus meets the synergistic requirements of the compound. Therefore, 4:1 (w / w) is determined to be the optimal compound ratio.

[0058] Example 4: Activity of a compound composition containing rotenone and Metarhizium anisopliae against Tetranychus carmine.

[0059] 1. Virulence determination against female adult mites

[0060] First, the test agent 5% Metarhizium anisopliae·rotenone dispersible oil suspension (1% rotenone, 4% Metarhizium anisopliae, 14% TENSIOFIX 96DB10, 3% Atlox-4914, 3% Atlas G-5002L, 4% Atlox Rheostrux-100, 0.5% ZnO, 70.5% soybean oil) was diluted 50 times, 100 times, 200 times, 400 times, 500 times, and 800 times respectively, using 0.1% Tween 80 solution to conduct indoor toxicity tests on Tetranychus carmine. Spray treatment was performed for 1 day, 2 days, and 3 days. After d, the mortality rate of *Tetranychus cinnabarinus* was corrected, with 20 female adult mites in each treatment. Two agents were used as positive controls: a 2000-fold dilution of 8 billion spores / mL *Metarhizium anisopliae* MaGX19S02 dispersible oil suspension and a 500-fold dilution of 6% rotenone microemulsion. An equal volume of water was used as a blank control. The number of dead mites was recorded, the corrected mortality rate was calculated, and the dead mites were transferred to petri dishes lined with filter paper. The mites' physical characteristics were used to determine whether the death was caused by fungal infection. The experimental results are shown in Tables 9 and 10. Figure 2 As shown.

[0061] Table 9. Mortality rates of different pesticides against Tetranychus carmine.

[0062] Note: Data in the table are mean ± standard deviation. Different letters after the data in the same column indicate the mean ± standard deviation. P The difference was statistically significant at levels <0.05.

[0063] The experimental results showed that when the 5% Metarhizium anisopliae·rotenone OD preparation was diluted 200 times or less, it had high insecticidal activity against Tetranychus carmineus. Compared with the recommended dose of 6% rotenone microemulsion and Metarhizium anisopliae preparation MaGX19S02 dispersible oil suspension alone, the effect was stronger (Table 9).

[0064] Table 10. Toxicity of 5% Metarhizium anisopliae·Rotenone Dispersible Oil Suspension against Tetranychus carmine

[0065] Table 10 shows the toxicity regression analysis of 5% Metarhizium anisopliae·rotenone dispersible oil suspension at 48 hours and 72 hours. 50 The values ​​ranged from 105.82 to 115.52 mg / L.

[0066] Depend on Figure 2 It can be seen that, Figure 2 After spraying with AB, 5% Metarhizium anisopliae·rotenone dispersible oil suspension diluted 100 times for 3 days, the condition of Tetranychus carmine was basically dead; Figure 2 CE, the state of the stunted insects after 5 days of moisturizing treatment; Figure 2 F, Morphology of *Metarhizium anisopliae* inoculated onto PDA plates. The results showed that after treatment with 5% *Metarhizium anisopliae*·rotenone dispersible oil suspension, the *Tetranychus cinnabarinus* mites shrank, and mycelia and spores grew on their surface. After inoculating the mites onto PDA medium and culturing for 3 days, *Metarhizium anisopliae* spores germinated into mycelia, and after 7 days of culture, a large number of spores were produced.

[0067] 2. Effect on inhibiting ovulation

[0068] from Figure 3 It can be seen that the 5% Metarhizium anisopliae·rotenone OD preparation has good oviposition inhibitory activity against adult female Tetranychus carmine. Under normal circumstances, due to the extremely strong reproductive capacity of Tetranychus carmine, the number of eggs laid increases almost linearly with the number of days.

[0069] The 5% Metarhizium anisopliae·rotenone OD preparation diluted 100 times and 200 times showed comparable and very high inhibitory activity against oviposition of Tetranychus carmine. After treatment with the solution, the number of eggs laid did not increase much in 1-3 days, and the oviposition inhibition rate was close to 100%, indicating that the solution at these two concentrations can effectively inhibit oviposition of Tetranychus carmine.

[0070] After treating *Tetranychus carmine* with a 400-fold dilution of 5% *Metarhizium anisopliae*·rotenone OD formulation, the number of eggs laid by female adult mites was higher than that of the 100-fold and 200-fold dilutions, but still much lower than that of the control group. The oviposition inhibition rate was low on day 1 after treatment, but increased on day 2, exceeding 70%, indicating that the 400-fold dilution of the OD formulation still has a certain inhibitory effect on oviposition of *Tetranychus carmine*.

[0071] The 5% Metarhizium anisopliae·rotenone OD preparation diluted 800 times showed the worst oviposition inhibition activity against Tetranychus carmineus. After treatment with the drug, the number of ovipositions laid by female adult mites was higher and the oviposition inhibition rate was the lowest. However, the oviposition inhibition rate increased with time during the 3-day experiment, reaching 65.18% on the 3rd day.

[0072] Example 5: Field experiment on Tetranychus carmine against a compound acaricide containing rotenone and Metarhizium anisopliae.

[0073] The experiment was conducted in the experimental field of the farm on campus of South China Agricultural University, using 90-day-old white kidney beans as the test crop. The experiment period was from June 5th to June 15th, 2025. The experimental treatments were 100x, 200x, 400x, and 800x dilutions of 5% Metarhizium anisopliae·rotenone dispersible oil suspension (1% rotenone, 4% Metarhizium anisopliae, 14% TENSIOFIX 96DB10, 3% Atlox-4914, 3% Atlas G-5002L, 4% AtloxRheostrux-100, 0.5% ZnO, and 70.5% soybean oil). Two agents, 8 billion spores / mL Metarhizium anisopliae MaGX19S02 dispersible oil suspension at 2000x dilution and 6% rotenone microemulsion at 500x dilution, were used as positive controls. An equal volume of water and an untreated group were used as blank controls. The plot area was 81 m², with each treatment replicated four times (each leaf counted as one replicate). The test agent was sprayed evenly using an electric sprayer (HM-16A, Zhongshan Hemei Electric Appliance Co., Ltd.), with a water volume of 40 liters per acre. Live mite counts were collected at 1, 5, 9, and 11 days after application to calculate the control effect.

[0074] Table 11 Control efficacy of 5% Metarhizium anisopliae·rotenone OD formulation against Tetranychus carmine.

[0075] The data in the table are mean ± standard error. Different letters after the data in the same column indicate the mean ± standard error. P The difference was statistically significant at a level <0.05 (DMRT method).

[0076] The results are shown in Table 11. The 5% Metarhizium anisopliae·rotenone OD formulation, when diluted 100 times, showed high control efficacy against Tetranychus carmine, with good rapid action. The control efficacy reached 77.46% one day after treatment, peaking at 83.90% on the fifth day. Furthermore, the efficacy was relatively long-lasting; although it began to decline after the fifth day, it remained at 74.87% until the eleventh day after treatment. This group exhibited the longest duration of efficacy and the most stable results. Compared with the positive control, the control efficacy of 6% rotenone microemulsion diluted 2000 times was 54.06% 1 day after application, rising to 69.83% after 5 days, and 59.02% after 11 days; the control efficacy of 8 billion spores / mL MaGX19S02 dispersible oil suspension diluted 500 times was 15.57% 1 day after application, rising to 27.80% after 5 days, and 26.07% after 11 days. It is evident that the 5% Metarhizium anisopliae·rotenone OD formulation has a higher control efficacy against Tetranychus carmine, and its rapid and sustained effects are significantly stronger than those of using 6% rotenone microemulsion alone or Metarhizium anisopliae formulation MaGX19S02 dispersible oil suspension alone.

[0077] A 200-fold dilution of 5% Metarhizium anisopliae·rotenone OD formulation showed low control efficacy 1 day after application, rising to 45.39% after 5 days, indicating that this dilution still had some control effect on Tetranychus carmine. However, the control efficacy began to decline after 9 days, reaching only 32.61% by day 11, showing a significant decrease in control effectiveness. Diluted formulations at 400 and 800 times showed poor control efficacy. The 400-fold dilution showed a control efficacy of 40% after 5 days, but this dropped to below 20% after 9 days. Even the 800-fold dilution did not exceed 40% control efficacy, indicating that this dosage was insufficient to effectively control Tetranychus carmine.

[0078] In summary, the experimental results show that the present invention provides a compound acaricide containing rotenone and Metarhizium anisopliae. The active ingredients rotenone and Metarhizium anisopliae have a synergistic effect on the control of Tetranychus cinnabarinus when the compound ratio is within a reasonable range.

Claims

1. A compound composition containing Metarhizium anisopliae and rotenone, characterized in that, The effective components of the compound composition are rotenone and Metarhizium anisopliae MaGX19S02, and the mass ratio of Metarhizium anisopliae MaGX19S02 to rotenone is 0.1-9:0.1-1.

2. The compound composition according to claim 1, characterized in that, The mass ratios of the compound of Metarhizium anisopliae MaGX19S02 and rotenone were 9:0.1, 2.3:0.1, 1:0.1, 4:1, and 1:

1.

3. The compound composition according to claim 2, characterized in that, The mass ratio of Metarhizium anisopliae MaGX19S02 to rotenone is 4:

1.

4. A dispersible oil suspension, characterized in that, The effective components of the dispersible oil suspension include Metarhizium anisopliae MaGX19S02 and rotenone as described in any one of claims 1-3; the dispersible oil suspension also includes additives, including emulsifiers, non-aqueous dispersants, aqueous dispersants, thickeners, UV protectants and oil bases.

5. The dispersible oil suspension agent as described in claim 4, characterized in that, The dispersible oil suspension comprises the following components by weight percentage: 0.1-9% Metarhizium anisopliae, 0.1-1% rotenone, 4-16% emulsifier, 2-3% non-aqueous dispersant, 2-3% aqueous dispersant, 4-5% thickener, 0.5-1% UV protectant, and the balance being oil base.

6. The dispersible oil suspension agent as described in claim 5, characterized in that, The dispersible oil suspension comprises the following components by weight percentage: 4% Metarhizium anisopliae, 1% rotenone, 14% emulsifier, 3% non-aqueous dispersant, 3% aqueous dispersant, 4% thickener, 0.5% UV protectant, and 70.5% oil base.

7. The dispersible oil suspension agent as described in claim 6, characterized in that, The emulsifier includes TENSIOFIX 96DB10, Atlox-4914, or Atlas G-5002L; the non-aqueous dispersant includes Atlox-4914; the aqueous dispersant includes Atlas G-5002L or dispersant 5040; the thickener includes TENSIOFIX-869, Atlox Rheostrux-100, or A200; the UV protectant includes TiO2, ascorbic acid, ZnO, fluorescent whitening agent, or sodium carboxymethyl starch; and the oil base includes soybean oil.

8. The dispersible oil suspension agent as described in claim 6, characterized in that, The dispersible oil suspension comprises the following components by mass percentage: 1% rotenone, 4% Metarhizium anisopliae, 14% TENSIOFIX 96DB10, 3% Atlox-4914, 3% Atlas G-5002L, 4% Atlox Rheostrux-100, 0.5% ZnO, and 70.5% soybean oil.

9. The use of the compound composition according to any one of claims 1-3 or the dispersible oil suspension according to any one of claims 4-8 in the prevention and control of agricultural pests.

10. The application as described in claim 9, characterized in that, The agricultural pest in question is the carmine spider mite.

Images